IMPROVED DIAGNOSISFOR ORAL MUCOSAL TUMORSIN THE DENTIST’S OFFICE

Based on currently available literature, clinical examination remains the major method when handling cases of suspected malignancy. However, this method does not allow diagnosing cancer, due to which a large group of patients with possible oral mucosa cancer are referred to an oncologist. The search and use of affordable non-invasive methods for early diagnosis of oral mucosa tumors is an urgent issue facing the health system. The study involved analyzing 134 records of outpatients examined at the Samara Regional Oncological Clinic who were referred by dentists within 2014-2019 from the local polyclinic in Samara due to detection of tumors in oral mucosa and who underwent a biopsy. The patients were divided into two groups according to the examination methods. The inclusion criteria were: detection of various superficial oral mucosa neoplasms; referral from the dentist. The exclusion criteria were as follows: patients with submucosal oral cavity neoplasms referred to the oncologist by other medical specialists or self-referred patients. The control group included 63 patients who, after a conventional examination (including interview, examination, palpation), underwent an incisional biopsy followed by morphological examination at the oncologist’s office. In the major group, in 71 patients at their respective initial dental appointments a special examination algorithm was applied. This algorithm entailed an assessment of the identified risk factors. Indications for biopsy were identified using the histological verification index (HVI). Apart from the conventional examination methods (interview, examination, palpation), autofluorescence stomatoscopy was used, this being done for the purpose of differential diagnostics of inflammation, precancerous and malignant issues, depending on the glow type. In the main group, the initial stages of oral mucosa cancer were detected in 17 patients after biopsy; in the control group – in 4 patients (p=0.004). The developed algorithm used for scoring the patient’s clinical examination data combined with autofluorescence stomatoscopy allowed diagnosing accurately (90% of reliability) precancerous and cancerous diseases, as well as to use invasive research methods (biopsy) strictly following the indications. Aim of study: to improve diagnosis of oral mucosa neoplasms through improvement of the examination algorithm.

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The research of the state of prosthetic bed tissues in patients with pemphigus vulgaris.

Archive of Clinical Medicine ◽

10.21802/acm.2017.1.11 ◽

2017 ◽

Vol 23 (1) ◽

Author(s):

Bohdan Lyubomyrovych Henyk ◽

Mykola Mykhaylovych Rozko

Keyword(s):

Survey Data ◽

Oral Mucosa ◽

Clinical Examination ◽

Comparative Evaluation ◽

Objective Assessment ◽

Pemphigus Vulgaris ◽

The State ◽

Control Group

The clinical examination of condition of tissues prosthetic bed was conducted in 20 patients with pemphigus vulgaris. The results are compared with survey data of 20 persons of control group without somatic pathology. It was conducted the analyzes of subjective and objective assessment of tissues prosthetic bed, the results of clinical examination and frequency of various pathologies of the oral mucosa membrane in the studied groups, conducted the comparative evaluation of dental indicators.

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Identification of the site for biopsy in oral mucosa cancer diagnostics

Science and Innovations in Medicine ◽

10.35693/2500-1388-2020-5-3-181-185 ◽

2020 ◽

Vol 5 (3) ◽

pp. 181-185

Author(s):

Andrei E. Orlov ◽

Aleksei G. Gabrielyan ◽

Oleg I. Kaganov ◽

Mikhail A. Postnikov ◽

Dmitrii A. Trunin ◽

...

Keyword(s):

Oral Mucosa ◽

Green Light ◽

Light Filter ◽

Cancer Diagnostics ◽

Main Group ◽

Control Group ◽

Incisional Biopsy ◽

Biopsy Procedure ◽

Significant Difference ◽

Histological Verification

Objective to refine the method of incisional biopsy in the diagnosis of oral mucosa cancer using the auto-fluorescent stomatoscopy. Materials and method.The study was conducted on the base of the Samara Regional Clinical Oncology Center. The inclusion criterion for patients was the diagnose of the oral mucosa cancer of various localization. Patients were divided into 2 groups. The main group included patients (n=43), who were being diagnosed for cancer with the help of optimized incisional biopsy of the oral mucosa formations, using the "AFS-400" autofluorescence complex and glasses with a green light filter for identification. The patients of the control group (n=46) received the standard biopsy procedure under direct vision. Results.The first incisional biopsies revealed cancer in 25 (54%) patients of the control group and in 36 (84%) patients of the main group. A histological verification of the diagnosis was necessary in 7 (16%) patients of the main group and required the second biopsy. In the control group, for the same purpose, 17 (37%) patients underwent the second biopsy and 4 (9%) patients required the third biopsy procedure. Exophytic-papillary forms of cancer were the most complex for histological verification. The primary biopsy of these cases was effective in 16 (37%) patients in the main group and in 8 (17%) patients in the control group (p = 0.036). In patients with initial stages of cancer (I-II), with the first incision biopsy, the histological verification of cancer was achieved in 16 (37%) cases in the main group and in 8 (17%) cases in the control group (p = 0.036). Conclusion.The use of the "AFS-400" autofluorescent complex and glasses with a green light filter for incisional biopsy of oral mucosal formations allows histological verification of cancer with the first biopsy in 84% of cases, including in stages I - II in 16 (37%) cases and in exophytic papillary forms in 16 (37%) cases. The significant difference was registered for the similar indicators of the control group (p = 0.036).

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THE LEVEL OF CYTOKINES IN THE SALIVA OF PATIENTS WITH BREAST CANCER

Problems in oncology ◽

10.37469/0507-3758-2019-65-6-825-831 ◽

2019 ◽

Vol 65 (6) ◽

pp. 825-831

Author(s):

Lyudmila Belskaya ◽

Viktor Kosenok

Keyword(s):

Breast Cancer ◽

Control Group ◽

Early Stages ◽

Promising Tool ◽

Urgent Task ◽

Advanced Stages ◽

New Biomarkers ◽

Histological Verification ◽

Control Study ◽

Biochemical Examination

Currently, the urgent task is to search for new biomarkers as a promising tool for early detection and monitoring of breast cancer. The aim of the study was to study the level of cytokines in the saliva of patients with breast cancer. In the case-control study volunteers participated, which were divided into 3 groups: the main (breast cancer, n = 43), the comparison group (fibroadenoma, n = 32) and the control group (conditionally healthy, n = 39). All participants were questioned; biochemical examination of saliva, histological verification of the diagnosis was carried out. Intergroup differences are estimated by a nonparametric criterion. It is shown that in the context of breast cancer, the level of cytokines (IL-2, IL-4, IL-6, IL-10 and IL-18) is increasing, except for IL-8, the content of which decreases compared to the control group. When the disease progresses by the nature of the dynamics, the parameters are divided into two groups: IL-2, IL-4, IL-18 and IL-6, IL-8, IL-10. For the first group of cytokines, there was a decrease in content during the transition from the early stages to the more common ones. For the second group, when passing from stages T1-2N0M0 to T1-2NjM0, the level of cytokines remains practically constant. In the future, the level of cytokines is observed for stage T3_4N0_2M0, and for IL-2, IL-4 and IL-10, the level of cytokines reaches values corresponding to early stages, whereas for IL-6, IL-8 and IL-18 in the same direction, a significant increase in indicators was noted. Additionally, the IL-6/IL-8 ratio was calculated depending on the tumor size, as well as the presence / absence of metastasis. It is shown that this ratio is statistically significantly increased in the advanced stages of the disease. Particularly interesting is the increase in this ratio in saliva at the initial stages of the disease.

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Relationship between Invasive and Non-Invasive Hemodynamic Measures in Experimental Pulmonary Hypertension

Current Respiratory Medicine Reviews ◽

10.2174/1573398x16666200516180118 ◽

2020 ◽

Vol 16 (1) ◽

pp. 47-53

Author(s):

Vicente Benavides-Córdoba ◽

Mauricio Palacios Gómez

Keyword(s):

Heart Rate ◽

Pulmonary Hypertension ◽

Ejection Fraction ◽

Right Ventricle ◽

Correlation Coefficient ◽

Pearson Correlation ◽

Systolic Pressure ◽

Control Group ◽

Pearson Correlation Coefficient ◽

Non Invasive

Introduction: Animal models have been used to understand the pathophysiology of pulmonary hypertension, to describe the mechanisms of action and to evaluate promising active ingredients. The monocrotaline-induced pulmonary hypertension model is the most used animal model. In this model, invasive and non-invasive hemodynamic variables that resemble human measurements have been used. Aim: To define if non-invasive variables can predict hemodynamic measures in the monocrotaline-induced pulmonary hypertension model. Materials and Methods: Twenty 6-week old male Wistar rats weighing between 250-300g from the bioterium of the Universidad del Valle (Cali - Colombia) were used in order to establish that the relationships between invasive and non-invasive variables are sustained in different conditions (healthy, hypertrophy and treated). The animals were organized into three groups, a control group who was given 0.9% saline solution subcutaneously (sc), a group with pulmonary hypertension induced with a single subcutaneous dose of Monocrotaline 30 mg/kg, and a group with pulmonary hypertension with 30 mg/kg of monocrotaline treated with Sildenafil. Right ventricle ejection fraction, heart rate, right ventricle systolic pressure and the extent of hypertrophy were measured. The functional relation between any two variables was evaluated by the Pearson correlation coefficient. Results: It was found that all correlations were statistically significant (p <0.01). The strongest correlation was the inverse one between the RVEF and the Fulton index (r = -0.82). The Fulton index also had a strong correlation with the RVSP (r = 0.79). The Pearson correlation coefficient between the RVEF and the RVSP was -0.81, meaning that the higher the systolic pressure in the right ventricle, the lower the ejection fraction value. Heart rate was significantly correlated to the other three variables studied, although with relatively low correlation. Conclusion: The correlations obtained in this study indicate that the parameters evaluated in the research related to experimental pulmonary hypertension correlate adequately and that the measurements that are currently made are adequate and consistent with each other, that is, they have good predictive capacity.

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Retinal vascular fractal dimensions and their association with macrovascular cardiac disease.

Ophthalmic Research ◽

10.1159/000514442 ◽

2021 ◽

Author(s):

Sebastian Dinesen ◽

Pia Søndergaard Jensen ◽

Maria Bloksgaard ◽

Søren Leer Blindbæk ◽

Jo G.R. De Mey ◽

...

Keyword(s):

Confidence Interval ◽

Cardiac Disease ◽

Artery Bypass ◽

Fractal Dimensions ◽

Macrovascular Disease ◽

Control Group ◽

Vascular Structure ◽

Non Invasive ◽

Automatic Software ◽

Multivariable Regression

Introduction As the only part of the human vasculature, retina is available for direct, non-invasive inspection. Retinal vascular fractal dimension (DF) is a method to measure the structure of the retinal vascular tree, with higher non-integer values between 1 and 2 representing a more complex and dense retinal vasculature. Retinal vascular structure has been associated with a variety of systemic diseases and this study examined the association of DF and macrovascular cardiac disease in a case-control design. Methods Retinal fundus photos were captured with Topcon TRC-50X in 38 persons that had coronary artery bypass grafting (CABG, cases) and 37 cardiovascular healthy controls. The semi-automatic software VAMPIRE was used to measure retinal DF. Results Patients with CABG had lower DF of the retinal main venular vessels compared to the control group (1.15 vs. 1.18, p=0.01). In a multivariable regression model adjusted for gender and age, eyes in the fourth quartile with higher DF were less likely to have CABG compared to patients in the first (OR, 7.20; 95% confidence interval, 1.63 to 31.86; p=0.009) and second quartile (OR, 8.25; 95% confidence interval, 1.70 to 40.01; p=0.009). Conclusions This study demonstrates that lower complexity of main venular vessels associates with higher risk of having CABG. The research supports the hypothesis that the retinal vascular structure can be used to assess non-ocular macrovascular disease.

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Variability of serum IgG sialylation and galactosylation degree in women with advanced endometriosis

Scientific Reports ◽

10.1038/s41598-021-85200-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Katarzyna Sołkiewicz ◽

Hubert Krotkiewski ◽

Marcin Jędryka ◽

Ewa M. Kratz

Keyword(s):

Cluster Analysis ◽

Sialic Acid ◽

Control Group ◽

Clinical Tool ◽

Non Invasive ◽

Healthy Women ◽

Serum Igg ◽

And Cluster Analysis ◽

Invasive Diagnostics ◽

And Control

AbstractEndometriosis is an inflammatory disease which diagnostics is difficult and often invasive, therefore non-invasive diagnostics methods and parameters are needed for endometriosis detection. The aim of our study was to analyse the glycosylation of native serum IgG and IgG isolated from sera of women classified as: with endometriosis, without endometriosis but with some benign ginecological disease, and control group of healthy women, in context of its utility for differentiation of advanced endometriosis from the group of healthy women. IgG sialylation and galactosylation/agalactosylation degree was determined using specific lectins: MAA and SNA detecting sialic acid α2,3- and α2,6-linked, respectively, RCA-I and GSL-II specific to terminal Gal and terminal GlcNAc, respectively. The results of ROC and cluster analysis showed that the serum IgG MAA-reactivity, sialylation and agalactosylation factor may be used as supplementary parameters for endometriosis diagnostics and could be taken into account as a useful clinical tool to elucidate women with high risk of endometriosis development. Additionally, we have shown that the analysis of native serum IgG glycosylation, without the prior time-consuming and expensive isolation of the protein, is sufficient to differentiation endometriosis from a group of healthy women.

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The Effect of a Non-Invasive Positive Pressure Ventilation Simulation Program on General Ward Nurses’ Knowledge and Self-Efficacy

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18062877 ◽

2021 ◽

Vol 18 (6) ◽

pp. 2877

Author(s):

Moon-Sook Kim ◽

Mi-Hee Seo ◽

Jin-Young Jung ◽

Jinhyun Kim

Keyword(s):

Self Efficacy ◽

Qualitative Data ◽

Positive Pressure Ventilation ◽

Intervention Group ◽

General Ward ◽

Simulation Program ◽

Positive Pressure ◽

Control Group ◽

Training Strategy ◽

Non Invasive

The purpose of this study is to develop a simulation-based ventilator training program for general ward nurses and identify its effects. Quantitative data were collected from 29 nurses (intervention group: 15, control group: 14), of which seven were interviewed with focus groups to collect qualitative data. The quantitative results revealed significant differences in ventilator-related knowledge (p = 0.029) and self-efficacy (p = 0.026) between the intervention and control groups. Moreover, three themes were derived from meaningful statements in the qualitative data: understanding psychophysical discomfort of the patient while applying the ventilator; helping in ventilator care; and establishing a future ventilator training strategy. The findings confirmed that the non-invasive positive pressure ventilation (NPPV) simulation program is an effective method for improving the knowledge of ventilator nursing and self-efficacy and will be helpful in developing educational methods and strategies related to ventilator nursing for general ward nurses.

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Characteristics of examination algorithm of patients with chronic trauma of the oral mucosa using tissue autofluorescence spectroscopy

Periodontology ◽

10.33925/1683-3759-2021-26-2-163-169 ◽

2021 ◽

Vol 26 (2) ◽

pp. 163-169

Author(s):

V. A. Gordeeva ◽

I. V. Kulik ◽

E. A. Khromova ◽

A. L. Rubezhov ◽

M. V. Gordeeva

Keyword(s):

Oral Mucosa ◽

Clinical Manifestations ◽

Diagnostic Techniques ◽

Diagnostic Methods ◽

Control Group ◽

Chronic Trauma ◽

Autofluorescence Spectroscopy ◽

Risk Zones ◽

Cancerous Lesions ◽

Group 5

Relevance. The paper demonstrates the need to implement modern diagnostic techniques for diagnosis of precancerous and cancerous lesions at early or preclinical stages. Additional diagnostic methods are necessary, e.g. tissue autofluorescence, which allows revealing insidious pathological risk zones, particularly precancerous and cancerous lesions, to evaluate the condition of the oral tissues in patients with chronic oral mucosa disorders, especially caused by trauma. Purpose – to assess trauma-specific effectiveness of autofluorescence spectroscopy (AFS) in risk group patients with chronic trauma of the oral mucosa to reveal early malignization signs.Materials and methods. 25 subjects were selected for the study and divided into 2 groups: main group – 20 patients with different manifestations of chronic oral mucosa trauma; control group – 5 subjects without visible clinical manifestations and without oral trauma factors. Autofluorescence spectroscopy was performed in both groups using AFS-400 stomatoscope.Results. The received data demonstrated that the change in autofluorescence doesn’t allow drawing final conclusions on the presence or absence of chronic oral trauma malignization signs.Conclusion. AFS-400 stomatoscope may be effective in differentiating between healthy and damaged tissues, but there is no solid evidence that the change in fluorescence shade can help differentiate between various types of damaged tissues. Autofluorescence spectroscopy should be considered as an additional method for examination of patients with chronic oral mucosa trauma to reveal early malignization signs.

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Capnography and Pulse Oximetry Improve Fast Track Extubation in Patients Undergoing Coronary Artery Bypass Graft Surgery: A Randomized Clinical Trial

10.21203/rs.3.rs-496561/v1 ◽

2021 ◽

Author(s):

Seyed Tayeb Moradian ◽

Fatemah Beitollahi ◽

Mohammad Saeid Ghiasi ◽

Amir vahedian-azimi

Keyword(s):

Clinical Trial ◽

Pulse Oximetry ◽

Fast Track ◽

Artery Bypass ◽

Bypass Graft ◽

Intervention Group ◽

Control Group ◽

Artery Bypass Graft ◽

Cabg Surgery ◽

Non Invasive

Abstract Background Use of capnography as a non-invasive method during the weaning process for fast track extubation (FTE) is controversial. We conducted the present study to determine whether pulse oximetry and capnography could be utilized as alternatives to arterial blood gas (ABG) measurements in patients under mechanical ventilation (MV) following coronary artery bypass graft (CABG) surgery. Methods In this randomized clinical trial, 70 patients, who were candidates for CABG surgery, were randomly assigned into two equal groups (n = 35); the intervention group and the control group. In the intervention group, the ventilator management and weaning from MV was done using Etco2 from capnography and SpO2 from pulse oximetry. Meanwhile, in the control group, weaning was done based on ABG analysis. The length of intensive care unit (ICU) stay, time to extubation, number of manual ventilator setting changes, and alarms were compared between the groups. Results The end-tidal carbon dioxide (ETCO2) levels in the intervention group were completely similar to the partial pressure of carbon dioxide (PaCo2) in the control group (39.5 ± 3.1 vs. 39.4 ± 4.32, P > 0.05). The mean extubation times were significantly shorter in the intervention group compared to those in the control patients (212.2 ± 80.6 vs. 342.7 ± 110.7, P < 0.001). Moreover, the number of changes in the manual ventilator setting and the number of alarms were lower in the intervention group (P < 0.05). However, the differences in the length of stay in ICU between the two groups were not significant (P = 0.219). Conclusion According to our results, the use of non-invasive monitors, including capnography and pulse oximetry, is emphasized in order to utilize FTE after CABG surgery. Furthermore, it is a safe and valuable monitor that could be a good alternative for ABG in this population. Nevertheless, further studies with larger sample sizes and on different disease states and populations are required to assess the accuracy of our findings. Trial registration: IRCT, IRCT201701016778N6, Registered 3 March 2017, https://www.irct.ir/trial/7192

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Effectiveness of hydrocolloid nasal dressing pads in preventing nasal ulceration in preterm infants using non-invasive ventilation

MedPharmRes ◽

10.32895/ump.mpr.5.4.6 ◽

2021 ◽

Vol 5 (4) ◽

pp. 46-51

Author(s):

Chau Vu Bao Nguyen ◽

Tinh Thu Nguyen ◽

Tam Thi Thanh Pham ◽

Sen Thi Hong Lam ◽

Le An Pham ◽

...

Keyword(s):

Preterm Infants ◽

Neonatal Intensive Care ◽

Nasal Cannula ◽

The Other ◽

Control Group ◽

Invasive Ventilation ◽

Nasal Bridge ◽

Non Invasive ◽

Hydrocolloid Dressing ◽

Non Invasive Ventilation

Background: The use of non-invasive ventilation (NIV) in preterm infants is becoming increasingly common. The use of cannula in NIV can cause ulceration of the nasal bridge with the current practices using the thin foam patches. This study aims to evaluate the effectiveness of hydrocolloid nasal dressing pads in preventing nasal ulceration comparing to that of the thin foam patches. Methods: A prospective cohort study using hydrocolloid dressing pads (1 November to 30 April 2020) was compared to that of a historical control group using thin foam dressing (1 April to 15 October 2019) to evaluate the effectiveness of hydrocolloid dressing pads. All participants were preterm infants (less than 37 weeks of gestational age) and used nasal cannula NIV at the Department of Neonatal Intensive Care (NICU), Children's Hospital 1. Results: 71 infants used hydrocolloid dressing pads, and 42 used ordinary thin foam nasal dressings. In the hydrocolloid dressings group, two infants (2.8%) had nasal ulcers; among them, one was mild, and the other was moderate. In comparison, ten infants (23.8%) using thin foam dressings developed ulcers, of which seven were mild, two were moderate, and one was severe. Using hydrocolloid nasal dressings significantly reduced nasal ulceration compared to thin foam dressings (OR = 0.09, 95%CI = 0.02 – 0.45). Conclusion: Using hydrocolloid nasal dressings for preterm infants on nasal cannula NIV significantly reduced nasal ulceration compared to ordinary thin foam dressings.

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