scholarly journals THE DYNAMICS OF LUNG HISTOPATHOLOGY IN ACUTE BACLOFEN POISONING

2021 ◽  
Vol 11 (4) ◽  
pp. 65-67
Author(s):  
Olga Romanova ◽  
Arkady Golubev ◽  
Alexey Churilov ◽  
Evgeny Barinov ◽  
Tatyana Chochlova
Keyword(s):  
Wistar Rats ◽  
Experimental Studies ◽  
Muscle Relaxants ◽  
Microcirculatory Bed ◽  
Psychotropic Effect ◽  
Lung Histopathology ◽  
Circulatory Disorders ◽  
Exact Moment ◽  
Experimental Group ◽  
Intact Rats

Baclofen (sold under the name Baclosan©, Lioresal©) is a muscle relaxant. This drug is chemically different from other muscle relaxants. Due to its pronounced psychotropic effect the drug is often a subject of abuse especially among young people. The article deals with the dynamics of lung histopathology in acute baclofen poisoning. Experimental studies were performed on 15 Wistar rats. The animals were divided into 3 groups (the controls and two experimental groups). The controls included 5 intact rats. Each experimental group included 5 animals. Both groups were treated with baclofen at a dosage of 85 mg/kg. The duration of the experiment was 3 and 24 hours, respectively. We revealed a complex of pathological changes in the lungs of the rats. There were circulatory disorders in all the elements of the microcirculatory bed, areas of emphysema, atelectasis and dystelectasis, WBC infiltration of intralveolar septa and their thickening due to edema. The changes were even more severe 24 hours after the drug administration. These results along with the results of chemical analysis will be useful in establishing the fact of baclofen intoxication and the exact moment of the intoxication.

scholarly journals CLOZAPINE AND CLOZAPINE-ETHANOL POISONING AS A CAUSE OF HISTOMORPHOLOGICAL CHANGESIN THE CE

2020 ◽  
Vol 10 (4) ◽  
pp. 69-71
Author(s):  
Alexey Churilov ◽  
Arkady Golubev ◽  
Dmitriy Sundukov ◽  
Olga Romanova
Keyword(s):  
Clinical Practice ◽  
Purkinje Cell ◽  
Purkinje Cells ◽  
Experimental Studies ◽  
Therapeutic Index ◽  
Severe Damage ◽  
Narrow Therapeutic Index ◽  
Irreversible Damage ◽  
Psychotropic Effect ◽  
Intact Rats

Clozapine is an atypical neuroleptic with a narrow therapeutic index which is widely used in clinical practice. Due to its psychotropic effect the drug is often misused. Experimental studies were performed on 25 outbreed mail rats. The animals were divided into 5 groups (the controls and four experimental groups). The controls included 5 intact rats. The experimental groups 1 and 2 were treated with clozapine at a dose 150 mg/kg. The experimental groups 3 and 4 received clozapine at the same dose and ethanol (2 ml/kg 70% ethanol). Severe damage to the Purkinje cells of the cerebellum was confirmed by an increase in the number of neurons that register signs of both reversible and irreversible damage. Purkinje cell alteration has increased by 24 hours from the beginning of the experiment.

scholarly journals HISTOMORPHOLOGICAL CHANGES IN THE LUNG AFTER BACLOFEN POISONING AND ITS COMBINATION WITH ETHANOL (EXPERIMENTAL RESEARCH)

2020 ◽  
Author(s):  
Olga L. Romanova
Keyword(s):  
Wistar Rats ◽  
Experimental Studies ◽  
Early Period ◽  
Acute Poisoning ◽  
Morphometric Study ◽  
Pathological Changes ◽  
Histomorphological Changes ◽  
Group 3 ◽  
Group 2 ◽  
Experimental Group

Aim. detection and assessment of histomorphological changes in the lungs in acute poisoning with Baclofen and its combination with ethanol 3 hours after the poisoning. Material and methods. Experimental studies were conducted on 15 Wistar rats divided into 3 groups. The group of controls -1 included 5 intact animals, group 2 was treated to Baclofen at a dosage of 85 mg/kg, group 3 was treated to combination of Baclofen (85 mg / kg) and ethanol (7 ml/kg 40% ethanol) Results. No pathological changes were observed in the lungs of the controls. In both experimental groups capillary and venous plethora, sludge, atelectasis and deselectasis, emphysema, infiltration of intraalveolar septi by WBC, thickening of intraalveolar septi due to edema were detected. Secretion was observed in the bronchioles of the second experimental group of animals. Conclusion. As a result of the study we identified a complex of pathological changes in the lungs of experimental animals in the early period after the administration of Baclofen, which included circulatory disorders in all the elements of the microcirculatory bed (plethora of capilaries, venules), emphysema, atelectases and distelectases, infiltration of intraalveolar septi by WBC and thickening of intraalveolar septi due to edema. In order to quantify the severity of histomorphological changes in the lungs a morphometric study is required.

scholarly journals GAMBARAN HISTOPATOLOGI PARU TIKUS WISTAR SETELAH DIBERI PAPARAN ASAP ROKOK

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Novera Herdiani ◽  
Endah Budi Permana Putri
Keyword(s):  
Oxidative Stress ◽  
Alveolar Macrophage ◽  
Cigarette Smoke ◽  
Wistar Rats ◽  
Positive Control ◽  
Negative Control ◽  
Control Treatment ◽  
Rat Lung ◽  
Dragon Fruit ◽  
Lung Histopathology

Abstract: Cigarette smoke is the main cause of lung obstruction. One of the obstructions in real that iscaused by cigarette smoke is oxidative stress. Oxidative stress trigger inflammation response occur andlung obstruction. Cigarette smoke able to cause lung histopathology changes like lungs obstruction onalveolus wall. Red dragon fruit extract tackle free radical then able to against the oxidative stress. Theobjective of this study to examines the image of rat lung histopathology under exposed cigarette smoke.Twenty four Wistar rats divided four groups: negative control, positive control, red dragon fruit extracttreatment of 7,2 g/200 g WB, and red dragon fruit extract10,8 g/200 g WB. Negative control only givenstandard feed. Positive control given standar feed and exposed 21 cigarette per day. Treatment groupgiven feed during 21 days. In the 22nd day rats be sacrificed, the lung taken out for observation andimage of rat lung histopathology changes by making lung organ histopathology preparationhematoxicillin Eosion (HE) staining and observed under magnification light microscope 400x. The endresult of the study indicate the finding of changes in lung histopathology such as obstruction level overthe lung tissue higher, alveolar macrophage covered alveoli after being exposed cigarette smoke. Groupwhich does not exposed cigarette smoke, lung alveolus macrophage in normal condition, there are noobstruction or alveolus macrophage occurred, its alveolar macrophage does no covering alveoli.Treatment group administered fruit dragon extract dose 10,8 g/200 g WB and dose 7,2 g/200 g WB seemalmost the same with negative control treatment. Conclusion of the research is cigarette smoke exposecan influence the number of alveolar macrophage on wistar rats. Suggestion very required furtherresearch on oxdative stress parameter.

scholarly journals Efficacy of Immobilized Prolonged Ammonium Antiseptics in Treatment of Purulent Wounds

2017 ◽  
Vol 10 (4) ◽  
pp. 269-276
Author(s):  
B S Sukovatykh ◽  
A Y Grigoryan ◽  
A I Bezhin ◽  
T A Pankrusheva ◽  
Yu Yu Blinkov ◽  
...  
Keyword(s):  
Sodium Salt ◽  
Group Treatment ◽  
Benzalkonium Chloride ◽  
Wistar Rats ◽  
Microbial Contamination ◽  
Comparison Group ◽  
Wound Area ◽  
Purulent Wounds ◽  
Male Wistar Rats ◽  
Experimental Group

Relevance. Purulent complications occur in 35-45% of patients with surgical diseases, lethality rate from these conditions achieves 25%. The purpose of the study. To investigate the wound process while treating the purulent wounds with immobilized forms of miramistin and benzalkonium chloride in gel of sodium salt of carboxymetylcellulose. Materials and methods. There were analysed results of experimental study of the wound process on 108 male Wistar rats. Experimental animals were divided into 3 groups, each group included 36 rats. In a comparison group ‘Levomekol’ ointment was used for treatment. In the first experimental group treatment was done by the ointment with the following composition: 0,01% solution of miramistin — 100 g, metronidazol –1,0 g, sodium salt of carboxymetylcellulose — 4,0 g, while in the second experimental group ointment composition included benzalkonium chloride 0,02 g, metronidazol — 1,0 g, sodium salt of carboxymetylcellulose — 4,0 g, purified water — up to 100,0 g. The wound process was evaluated by planimetric, microbiological and histological methods of investigation. Results and its discussion. The reduction of wound area in the first experimental group was higher than in a comparison group on the 3rd day of the experiment by 9,7%, on the 5th day — by 7,6%, on the 10th and 15th days — by 10,5%, while in the second experimental group the change was 37,2%, 27,3%, 13,8% and 6,9% respectively. In the first experimental group microbial contamination of the wound was less than in a comparison group by 5,8х106, on the 5th day — by 3,1х105, on the 10th day — by 6,1х104, while in the second experimental group on the 3rd day there were no differences, on the 5th day — by 6,3х105, on the 10th day — 1,1х104. Histological investigations of the wound biopsies on the 10th day of experiment showed that in a comparison group the bottom of the wound was covered by granulation tissue without signs of epithelization, there was full coverage of the wound with a newly formed epithelium in experimental groups. Conclusions. The use of prolonged quaternary ammonium antiseptics in treatment of purulent wounds is pathogenetically justified and efficient.

Specific Treatments for Improving the Quality of Life in Rheumatologic Affections and Osteoporosis

2011 ◽  
Author(s):  
Petru A. Pop ◽  
Liviu Lazar ◽  
Florin M. Marcu
Keyword(s):  
Conservative Treatment ◽  
Wistar Rats ◽  
Vibration System ◽  
Mineral Density ◽  
Digital Frequency ◽  
Quality Life ◽  
Sf 36 ◽  
Life Scale ◽  
Circulatory Disorders

The analysis and implementation of modern treatments for the patients diagnosed with osteoporosis, degenerative rheumatic affections and peripheral circulatory disorders have become a healthy priority for the society. These treatments have the goal to raise the bone mineral density of the skeleton, reducing joints pain and inflammation while improving and maintaining joints function. This paper presents a conservative treatment of patients from Recovery Clinical Hospital of Felix Spa, diagnosed with degenerative and rheumatoid affections. The treatment is complex and involves balneal-physical-kinetic recovery therapy that must be periodical repeated at every six months to perform. Ankylosing spondylitis was been evaluated by BASFI-BASDAI scores, while the gonarthrosis with Artroflex by SF-36 of quality life scale and Lequesne index. In addition, a vibration treatment combined with balneal-conservative treatment was been proposed to reduce the therapy time and improved the quality live of patients. The vibration system comprises a vibration bed with adjusting control of signal by a digital frequency convertor, and a command panel system for measuring and processing of data. Because the vibration trial is in incipient experiment stage, it was been applied first on Wistar rats, due to increase the calcium mass of bones. The results of all treatments were emphasized the efficiency of balneal cure in rheumatic affections.

scholarly journals Bisphosphonate’s Effect on Tongue Mucosa: An Experimental Electron Microscopy Study

Medicina ◽  
2020 ◽  
Vol 56 (2) ◽  
pp. 51
Author(s):  
Theodora Papamitsou ◽  
Antonios Morsi-Yeroyannis ◽  
Anastasios Papanastasiou ◽  
Nikolaos Bakalopoulos ◽  
Eva-Maria Dietrich ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Intercellular Space ◽  
Wistar Rats ◽  
Middle Layer ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Control Group ◽  
The Past ◽  
Transmission Electron ◽  
Experimental Group

Background and objectives: Bisphosphonates (BPs) are selective inhibitors of osteoclasts, used for the treatment of bone disorders. The objective of this study is to investigate the possible effects of BPs on the tongue’s mucosa. Materials and Methods: Specimens of the tongue of 20 female 12-month old Wistar rats were taken. Ten were used as control group, while in the remaining alendronate (Fosamax, Merck) was administered per os from 13 weeks. Observation of the harvested samples was made by Transmission Electron Microscopy (TEM). Results: In the experimental group, focal alterations were observed to various extent in all specimens. The basement membrane was intact. Furthermore, an increase at the intercellular space was observed, predominantly at the middle layer, and the desmosomes were disorganized. In the lamina propria focal edema was observed. Conclusions: Investigation on the effect of BPs on the tongue’s mucosa through TEM hasn’t been documented in the past. According to our results, BPs seem to cause mild mucosal lesions on the tongue.

scholarly journals Electro-acupuncture reduces the need for additional anesthetics in experimental studies

2010 ◽  
Vol 25 (4) ◽  
pp. 381-384 ◽  
Author(s):  
Agamenon Honório Silva ◽  
Alberico Ximenes do Prado Neto ◽  
Sérgio Botelho Guimarães
Keyword(s):  
Stainless Steel ◽  
Electrical Stimulation ◽  
Wistar Rats ◽  
Experimental Studies ◽  
Needle Insertion ◽  
Additional Treatment ◽  
Human Right ◽  
Beneficial Effects ◽  
Group Control ◽  
Male Wistar Rats

PURPOSE: To evaluate the possible beneficial effects of electro-acupuncture in rats subjected to ketamine/xylazine (KX) intra-peritoneal (i.p.) anesthesia. METHODS: Forty-eight male Wistar rats were distributed in four equal groups. All rats received i.p. injections of ketamine (90 mg/kg) +xylazine (10 mg/kg) anesthesia. Basal values group (control) rats (BV) received no additional treatment. The equivalent of the human right ST36 (Zusanli) and CV-12(Zhongwan) acupoints were chosen for needling and electrical stimulation. AC rats were needled with sterilized disposable stainless steel needles at right ST36 and CV12 acupoints; needles were retained for 30 minutes. EAC10 rats, after needle insertion as described, had electrodes connected to both needles and to an electro stimulator model NKL EL-608; pulsed square waves, 10 Hz, 10 mA, was applied for 30 minutes. EAC100 rats were submitted to EA as described. However, a greater frequency (100 Hz) was used. RESULTS: Thirty-seven rats remained under adequate anesthetic level during the experiment. However, maintenance anesthesia was required by 11 rats. Need for additional anesthesia decreased to 9.1% in EAC100 rats compared to BV (36.3%). CONCLUSION: Both the AC and the EAC10/100 prolong the anesthetic effect of the combination Ketamine-xylazine in rats, allowing longer duration of anesthesia with a lower dose of anesthetic, thereby reducing the occurrence of complications.

Effect of Gmelina arborea on learning and memory in amnesia-induced and non-amnesia groups of albino Wistar rats

2019 ◽  
Vol 30 (5) ◽  
Author(s):  
Ravi Sori ◽  
Basavaraj Poojar ◽  
Nandan Hodlur ◽  
Priya Gandigawad
Keyword(s):  
Learning And Memory ◽  
Wistar Rats ◽  
Experimental Studies ◽  
Intellectual Ability ◽  
Screening Tests ◽  
Control Group ◽  
Significant Activity ◽  
Gmelina Arborea ◽  
Memory Processes ◽  
Anti Oxidant

Abstract Background The brain is the centre of the nervous system in all vertebrates. The central cholinergic pathways play a prominent role in learning and memory processes. Dementia is a mental disorder characterized by the loss of intellectual ability, which invariably involves the impairment of memory. The crude extracts of the Gmelina arborea plant are reported to possess wound-healing, anti-diarrheal, anti-oxidant, anti-diabetic, and anti-ulcer property. The present study was designed to evaluate the effects of G. arborea on learning and memory in albino Wistar rats. Methods A total of 36 healthy rats were selected for the study, which were divided in to six groups. Standard screening tests such as the elevated plus maze (EPM), Morris water maze (MWM), and step-down passive avoidance (SDA) tests were used for testing the learning and memory processes. Results Gmelina arborea at higher doses (1000 mg/kg) showed statistically significant activity in EPM, MWM, and SDA tests for assessing the learning and memory paradigms when compared to the control group in amnesia-induced and non-amnesia groups of rats. Conclusions This is the first ever study to report the effects of G. arborea on learning and memory in both amnesia-induced and non-amnesia groups of rats. Our results show that G. arborea potentiates the processes of learning and memory. The observed pharmacological activities should be further evaluated by detailed experimental studies and revalidated by clinical trials.

scholarly journals Consequences of Dental Occlusion Enhancement by Means of Metal Crowns on the Animal Model

2019 ◽  
Vol 69 (12) ◽  
pp. 3517-3519
Author(s):  
Ana Ispas ◽  
Antarinia Craciun ◽  
Liana Lascu ◽  
Marcela Elisabeta Barbinta Patrascu ◽  
Mariana Constantiniuc
Keyword(s):  
Oxidative Stress ◽  
Animal Model ◽  
Free Radicals ◽  
Wistar Rats ◽  
Vertical Dimension ◽  
Dental Occlusion ◽  
Control Group ◽  
Experimental Group ◽  
Plasma Malondialdehyde

The purpose of this study was to determine whether there is a correlation between induced occlusal trauma and the occurrence of oxidative stress in the hippocampus. Twenty Wistar rats were randomized into three experimental groups and one control group. Animals in the experimental group were cemented modified crowns on molars to induce occlusal trauma in 7, 14 and 30 days. To evaluate the oxidative stress, blood was drawn from the hippocampus at 7, 14 and 30 day intervals. Oxidative stress was evaluated using the following specific tests: determination of plasma malondialdehyde and serum glutathione values. The results of the study demonstrate that malocclusion experienced by raising the vertical dimension in rats resulted in significant reductions in antioxidants and increase level of free radicals.

scholarly journals Technical report: Simple method of animal stroke model of luminal occlusion of middle cerebral artery in Indonesia

2019 ◽  
Vol 10 ◽  
pp. 143
Author(s):  
Feda Anisah Makkiyah ◽  
Wismaji Sadewo
Keyword(s):  
Middle Cerebral Artery ◽  
Cerebral Artery ◽  
Wistar Rats ◽  
Experimental Studies ◽  
Artery Occlusion ◽  
Stroke Model ◽  
Simple Method ◽  
Technical Report ◽  
Male Wistar Rats ◽  
Cerebral Artery Occlusion

Background: Although there are many experimental studies describing the suture method of middle cerebral artery occlusion (MCAO) in rats, this is still a new procedure in Indonesia and the techniques for applying this stroke model in animal research are not well known. There has been a perception in Indonesian scientific community that the technique would be difficult and require advanced equipment. The aim of this study is to demonstrate that it is possible to perform the technique with minimal resources using simple method and basic surgical loupe equipment. Methods: A total of 30 male Wistar rats, aged 6 months, weighing 250 g–400 g Wistar rats, were obtained from the Bandung Biofarma Pasteur. Preliminary trials were performed to gain an understanding of the detailed anatomy of the animals and to master the techniques. An ×8 loupe magnification was used for all surgical steps in this study except taking of surgical operation photos. The procedures applied simple methods, using two loop temporary knots instead of any animal vascular clips. Results: After an extensive training period, two of the 30 rats died within 4 weeks after the procedure. The effects of MCAO were confirmed clinically and by hematoxylin-eosin staining pathology slides. Conclusion: With simple methods, this MCAO procedure could be implemented in developing countries such as Indonesia without the requirement for advanced equipment.

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