scholarly journals The Effect of Antioxidant Vitamin C and E Administration on Spermatozoa Quality of White Rat (Rattus Norvegicus) Strain Wistar Male Against Allethrin Exposure

2018 ◽  
Vol 1 (2) ◽  
pp. 113
Author(s):  
Faisal Yusuf Ashari ◽  
Rina Yudiwati ◽  
R.Haryanto Aswin
Keyword(s):  
Vitamin C ◽  
Rattus Norvegicus ◽  
The Body ◽  
Control Group ◽  
Antioxidant Vitamin ◽  
Control Group Design ◽  
Spermatozoa Motility ◽  
Treatment Groups ◽  
The Mean

Introduction: Allethrin is an active ingredient used in anti-mosquito drugs. Allethrin enters the body through inhalation, allegedly can disrupt the quality of spermatozoa by decreasing its amount, motility, viability and morphology. Many studies suggested that with sufficient intake of vitamin C and E would be able to minimize free radicals caused by allethrin. This study aims to investigate the effect of giving antioxidant vitamin C and E on spermatozoa quality of white rat (Rattus norvegicus) strain Wistar male with allethrin exposure.Methods: This research was a post-test design randomized control group design using Rattus novergicus male strains Wistar exposed to allethrin (LPB IPB veterinary stem cell). A total of 16 white rat strains Wistar male divided into 4 groups, one control group (without any treatment) and 3 treatment groups (given antioxidant vitamin C, vitamin E and both). The exposure of mosquito coils was performed 8 hours per day for 45 days and on the 46th day, spermatozoa was observed. The data were analyzed using ANOVA one way and BNT.Results: The results showed that treatment with vitamins affected the amount, motility and viability of rat spermatozoa exposed to allethrin. The mean amount of control group spermatozoa and treatment was 14,93, the mean spermatozoa motility control and treatment groups were 45,13 and the mean spermatozoa viability of control and treatment groups was 58,88. This is shown by the real difference between the control group and the treatment (p <0,05). Similarly, in the control group found morphological abnormalities.Conclusion: Exposure of mosquito coils 8 hours/day for 45 days decreased the spermatozoa quality. Overall, the admission of antioxidant vitamin C and E are able to maintain the quality of spermatozoa instead of one type of vitamin.

scholarly journals The Effect of the Separation of Spermatozoa X and Y Using Swim up Method on the Quality of Etawah Crossbreed Goats Spermatozoa

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Eskayanti Pasaribu ◽  
Dasrul Dasrul ◽  
Ginta Riady
Keyword(s):  
Semen Quality ◽  
Control Group ◽  
Spermatozoa Motility ◽  
Treatment Groups ◽  
Multiple Test ◽  
Group 3 ◽  
The Mean ◽  
Group 2 ◽  
Method Group

This research aimed to determine the effect of spermatozoa X and Y separation using swim-up method on the quality of etawah crossbreed goat’s spermatozoa. This research used 6 male etawah crossbreed goats aged between 18-24 months, which were collected the semen once a week using electroejaculator. Immediately after semen collection, the quality of semen was examined, and then grouped into three treatment groups. Group 1 was control group (P0) refers to the semen was not separated by swim-up method. Group 2 (P1) was the semen separated by the swim-up method for 5 minutes. Group 3 (P2) was the semen separated by swim-up method for 10 minutes. Each treatment was repeated six times. Parameters for semen quality examined were the percentage of spermatozoa motility, live spermatozoa, and abnormal spermatozoa. Data were analyzed by analysis of variance (ANOVA) one way pattern, followed by Duncan's multiple test. The results showed that the mean ± SD percentage of sperm motility on each treatment groups (P0; P1; P2) was 81.33±3.44, 89.67±3.21, and 90.00±3.10 %, respectively. The percentage of live spermatozoa on each group was 86.50 ± 2.07, 92.33 ± 2.08, and 91.83 ± 1.72%, respectively. The percentage of abnormal spermatozoa on each group was 13.00±2.53, 7.33±2.52, and 7.67±2.16%, respectively. Results of this research showed that the percentages of motility, live spermatozoa, and abnormal spermatozoa after separation by swim-up method were significantly different (P0.05) compared to without separation. It can be concluded that the separation of spermatozoa using swim-up method can improve significantly (P0.05) the percentage of live and motility of spermatozoa and decrease the percentage of abnormal spermatozoa of etawah crossbreed goats.Key words: Etawah crossbreed, spermatozoa quality, swim-up method

scholarly journals Pengaruh Kalsium Klorida (CaCl2) dan Lama Penyimpanan terhadap Kadar Vitamin C Anggur (Vitisvinifera)

2015 ◽  
Vol 1 (2) ◽  
pp. 61
Author(s):  
Anny Thuraidah ◽  
Haitami Haitami ◽  
Akhmad Dairobi
Keyword(s):  
Vitamin C ◽  
Calcium Chloride ◽  
Control Group ◽  
Blood Levels ◽  
Control Group Design ◽  
Treatment Groups ◽  
Duration Of Storage ◽  
Effect Of Treatment ◽  
And Storage

Vitisvinifera is one of the perishable fruit and not durable when stored at room temperature. To maintain the levels of vitamin C wine then used treatment using calcium chloride (CaCl2). This study aims to determine the effect of treatment Calcium chloride (CaCl2) and duration of storage of the vitamin C content of Vitisvinifera This type of research uses experimental methods actual (true experiment) in the form of draft posttest only control group design. Wine and CaCl2 research materials. Data were obtained by performing assays using titration iodometry vitamin C in grapes. Results of statistical analysis to test multiple linear regression R2 values ​​obtained showed no effect of treatment 0.578 Calcium chloride (CaCl2) and storage time for blood levels of vitamin C Vitisvinifera. From Duncan statistical test obtained significant value for all subsets of the 2 treatment groups of more than 0.05, the most optimal levels of vitamin C in maintaining the Vitisvinifera is a CaCl2 concentration of 0.05 M for 4 days of storage. Therefore, this study suggested as alternative preservation for merchants and producers / grape growers in order to maintain the vitamin C content of wine so as to improve the quality and the quality of the fruit.

scholarly journals The Effect of Propanolol on C-Reactive Protein in Patients with Severe Burns at Dr Soetomo Hospital Surabaya

2021 ◽  
Vol 4 (1) ◽  
pp. 27
Author(s):  
Muhammad Aulia U H ◽  
Iswinarno Doso Saputro ◽  
Magda Rosalina Hutagalung
Keyword(s):  
Treatment Group ◽  
Albumin Level ◽  
Mean Value ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Severe Burns ◽  
Hypermetabolic State ◽  
Post Test ◽  
The Mean

Background: The incidence of burns in Indonesia progressively increases with the increase in its population and industries. From January to September 2000, 158 patients were treated in the burn unit of Dr Soetomo Hospital with a mortality rate reaching 5,8%. Burns have a direct effect in causing both local and systemic changes in the body, not occurring in other injuries. In severe burns, a hypermetabolic state can occur, which increases cardiac workload and causes muscle atrophy and other morbidities. The purpose of this study is to examine the effect of propranolol on the hypermetabolic state in severely burned patients by measuring various clinical & laboratory parameters.Methods : This is an experimental study using pre and post test control group design with the objective of assessing the treatment outcome with oral propranolol given in 15 consecutive days for burn patients involving 25%- 60% TBSA. Measurements were taken three times, on day 0, 7 and 14.Results : Obtained 16 samples divided into 2 groups. In the treatment group, there was a significant decrease in CRP levels on days 0, 7 and 14 (p <0.05). The Mid Arm Circumference variable did not obtain a significant decreasing on days 0, 7 and 14. The albumin level studied showed a significant decreasing on day 0 & 7 days with a value of p = 0.045. From the comparison between the two groups, there were significant differences in CRP levels on days 0 & 14, with the mean value of the treatment group -5.12 + 2.88 and the mean value of the control group 2.86 + 7.37, and the value of p = 0.019.Conclusions: This study successfully proved that the administration of propranolol can overcome the effects of hypermetabolism which is characterized by decreasing in CRP levels.

scholarly journals Effects of Red Fruit (Pandanus conoideus Lam) Oil on Malondialdehyde Level and Spermatozoa Quality in Mice (Mus musculus) Exposed to Monosodium Glutamate

2018 ◽  
Vol 54 (2) ◽  
pp. 84 ◽  
Author(s):  
Widayati Agustina ◽  
Widjiati Widjiati ◽  
Alfiah Hayati
Keyword(s):  
Mus Musculus ◽  
Monosodium Glutamate ◽  
Control Group ◽  
Distilled Water ◽  
Control Group Design ◽  
Treatment Groups ◽  
Group Design ◽  
Post Test ◽  
Fruit Oil

This study aimed to determine the effects of red fruit (Pandanus conoideus Lam) oil on MDA levels and spermatozoa quality in mice (Mus musculus) exposed to MSG. The quality includes motility, viability, concentration, and morphology of spermatozoa. This experimental study used randomized post-test only control group design. The subjects of this study were 25 mice (Mus musculus), divided into 5 groups (5 mice per group). K- group received distilled water for 35 days. K+ group received 4 mg/g BW MSG for 21 days. P1, P2, and P3 treatment groups received 4 mg/g BW MSG for 21 days and 0.02; 0.04; 0.08 ml/g BW red fruit oil, respectively, from day 22 to 35. The results showed that mean spermatozoa morphology in K-, K+, P1, P2, P3 groups were as follows: 0.86; 0.56; 0.67; 0.61; and 0.87 (%). The spermatozoa concentrations were sequentially as follows: 21; 10; 15; 32,8,19 (107 cells/ml). The spermatozoa's vitalities were as follows: 0,64; 0,14; 0,24; P2: 0.36; 0.68 (%). MDA levels were respectively: 0.29; 0.60; 0.35; 0.23; and 0.19 (nm). As a conclusion, testicular MDA levels in mice exposed to MSG and given with red fruit oil were lower than those in mice exposed to MSG without receiving red fruit oil. The quality of spermatozoa in mice exposed to MSG and receiving red fruit oil was higher than that of mice exposed to MSG without being given with red fruit oil.

scholarly journals The Difference of Results in Abdomen and Anus Area Measurement With Non-Contact Infrared Thermometer

2021 ◽  
Vol 22 (2) ◽  
pp. 183-188
Author(s):  
Anna Surgean Veterini ◽  
Nancy Margarita Rehatta ◽  
Hamzah Hamzah ◽  
Widijiati Widijiati ◽  
Sarmanu Sarmanu ◽  
...  
Keyword(s):  
Animal Model ◽  
Body Temperature ◽  
Mean Difference ◽  
The Body ◽  
Area Measurement ◽  
Control Group ◽  
Control Groups ◽  
Infrared Thermometer ◽  
Treatment Groups ◽  
The Mean

Research about sepsis in animal model is interesting to get the right therapeutic method for human being. We did the research to study about the value of body temperature in mice’s various body area in order to get the important information if we are going to do the translational research in animal model about sepsis. One clinical signs of the sepsis mice model is changes in body temperature. An easy way to examine body temperature is using infrared non-contact thermometer. This study aimed to compare the body temperature using infrared non-contact thermometer at the abdomen and anal area. We used male mice, weighing 25–30 g, divided into two groups (control and treatment groups). The control group injected with NaCl 0.9% solution, with the amount of NaCl 0.9% volume equal to Lipopolysaccharide (LPS). In the treatment group injected with 2.5 mg/kg BW of LPS intraperitoneally. Body temperature was measured in abdomen (tabd) and ananl (tan) area at 8th and 24th hour after treatment. Body temperature value tabd was higher than tan. Lipopolysaccharide injection increase body temperature but was not significant when compared to the control group (8th and 24th hour). The mean difference between tabd and tan in 8th control groups were 2.12oC respectively. The mean difference between tabd and tan in 24th hour control groups 4.6oC. The mean difference in treatment groups (8th hour) was 4.66oC, while it was 4.77oC in the 24th groups. Giving 2.5 mg/kg BW LPS intraperitoneally did not rise the body temperature significantly as compare to control groups. But, body temperature at anus area using non-contact infrared thermometer after treatment showed lower results as compared to that of at abdomen significantly.

scholarly journals Pengaruh Pemberian Ekstrak Rumput Laut Cokelat (Sargassum duplicatum) terhadap Peningkatan Kadar Hematokrit pada Darah Tikus Putih Jantan Galur Wistar (Rattus norvegicus) Anemia yang di Induksi NaNO2

2020 ◽  
Vol 3 (2) ◽  
pp. 38
Author(s):  
Ayu Mayang A. P. ◽  
Liliawanti Liliawanti ◽  
Wachjudi Kurnia
Keyword(s):  
Rattus Norvegicus ◽  
White Male ◽  
Brown Seaweed ◽  
Negative Control ◽  
Laboratory Method ◽  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference

<p>Anemia is still a high prevalence disease in Indonesia, caused by a lack of iron in the body. The components from brown seaweed such as cobalamin (vitamin B12), pyridoxine (vitamin B6), chlorophyll and iron (Fe) can increase red blood cells in the body. The purpose of this research was to determine the effect of brown seaweed (<em>Sargassum duplicatum</em>) which can increase hematocrit levels in the blood of white male wistar strain rat (<em>Rattus norvegicus</em>) suffered from anemia in induction NaNO<sub>2</sub>.</p><p>            This research uses an experimental laboratory method with a post test only control group design. The samples were 30 male rats divided into 3 treatment groups, the negative control group (K<sub>0</sub>) without treatment, the positive control (K<sub>1</sub>) be treated anemia given NaNO<sub>2</sub> for 18 days without <em>Sargassum duplicatum </em>extract and treatment group (K<sub>2</sub>) treated with anemia given NaNO<sub>2</sub> for 18 days without <em>Sargassum duplicatum </em>extract at a dose of 2,45gr / kgBB. On the 40<sup>th</sup> day, a blood sample was taken, then measured the hematocrit level.</p><p>            The results showed that the mean number of blood hematocrit levels in K<sub>2</sub> was higher than K<sub>1</sub>, it showed a significant difference descriptively. Based on the Kruskal-Wallis test, a value of 0.260 was obtained that p &gt; 0,05 showed that there were no statistically significant differences between groups of experimental animals.</p><p>            From the results of this research, the conclusion is the administration of brown seaweed extract (<em>Sargassum duplicatum</em>) descriptively increased, but not statistically significant.</p><p> </p><p><strong>Keywords :</strong> Anemia, hematocrit, <em>Sargassum duplicatum</em>, NaNO<sub>2</sub></p>

scholarly journals Pengaruh Latihan Renang Teratur dan Latihan Renang Tidak Teratur Terhadap Kadar Trigliserida Plasma pada Rattus norvegicus

2018 ◽  
Vol 2 (1) ◽  
pp. 24-40
Author(s):  
Adi Wijayanto
Keyword(s):  
Rattus Norvegicus ◽  
Exercise Program ◽  
The Body ◽  
Control Group ◽  
Regular Exercise ◽  
Control Group Design ◽  
Group Design ◽  
Significant Difference ◽  
Post Test ◽  
And Control

Triglycerides are source of energy in the body, but triglyceride deposits that exceed the threshold will cause health problems, so triglycerides should be lowered to normal. The reduction of triglycerides can be done through physical activity, therefore this study aims to reveal the effect of regular swimming exercises and irregular exercises against triglyceride levels. This research is an experimental research using The Post Test Only Control Group Design design. Subjects of the study were male of Rattus Norvegicus Wistar, 39 subjects was divided into 3 groups with Matched Subject Design technique, which consisted of 2 groups of treatment that is regular and irregular group, and control group. The exercise program was given a swimming with a 3% weight of body weight and was tied 5 cm from the tip of the tail which was performed 3 times per week consistently for regular exercise and 0 to 5 times per week inconsistently for irregular exercise for 12 weeks, While the control group was not given training. In the last week before the blood was taken, the first three groups were given swimming treatment exercises. Based on the results of the anava test, there was a significant difference in the reduction of triglyceride levels among white mice who were given regular exercise with irregular exercise. These results suggest that regular exercise has a better impact on triglyceride reduction than irregular exercise, due to the higher adaptation process of regular exercise.

scholarly journals Pemberian bisphenol A (BPA) oral dapat menurunkan kadar testosteron pada tikus (Rattus norvegicus) jantan galur Sprague Dawley

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Nuriyah . ◽  
IGM Aman ◽  
Wimpie Pangkahila
Keyword(s):  
Bisphenol A ◽  
Testosterone Level ◽  
Rattus Norvegicus ◽  
Endocrine Disruptor ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Testosterone Levels ◽  
Group Design ◽  
The Mean

Abstract: Bisphenol A (BPA) has the structural and physiological activity of estrogen and acts as an endocrine disruptor. This study was aimed to prove that oral BPA could decrease testosterone levels in male Sprague Dawley rats. This was an experimental study with a randomized pretest-posttest control group design using 14 rats (Rattus norvegicus), male, Sprague Dawley strain, 2-3 months old, as subjects. Rats were divided into two groups with 7 rats each. The control group (P0) was treated with placebo for 14 days and the treatment group (P1) was treated with oral bisphenol A (BPA) single dose of 400mg/kg/day for 14 days (P1). At before and after 14-day treatment, blood was drawn through the canthus medial of orbital sinus to obtain the pretest and posttest data. Testosterone levels were examined by using ELISA. The results showed that before treatment (pretest), the mean testosterone level in P0 group was 8.29±4.68 nmol/L, while in P1 group was 7.39±1.45 nmol/L (P > 0.05). After treatment for 14 days, the mean testosterone level in P0 group was 7.40±1.37 nmol/L, while in P1 group was 3.36±1.26 nmol/L (P < 0.01). The statistical analysis showed that there was no difference in testosterone levels in P0 group (P > 0.05), meanwhile there was a very significant decrease of testosterone levels in P1 group (P < 0.01) after the treatment of BPA 400mg/kg/day for 14 days. Conclusion: Oral bisphenol A (BPA) could decrease testosterone levels in male rats (Rattus norvegicus) of Sprague Dawley strain.Keywords: bisphenol A (BPA), testosteroneAbstrak: Bisphenol A (BPA) memiliki aktifitas hormon estrogen sehingga jika masuk ke dalam tubuh dapat menjadi endocrine disruptor. Penelitian ini bertujuan untuk membuktikan pemberian BPA oral dapat menurunkan kadar testosteron pada tikus (Rattus norvegicus) jantan galur Sprague Dawley. Jenis penelitian ialah eksperimental dengan randomized pretest-postest control group design yang menggunakan 14 ekor tikus (Rattus norvegicus) jantan, galur Sprague Dawley, berumur 2-3 bulan, yang terbagi menjadi 2 (dua) kelompok masing-masing berjumlah 7 ekor tikus. Kelompok kontrol (P0) diberikan plasebo 14 hari dan kelompok perlakuan (P1) diberi BPA dosis tunggal 400mg/kgBB/hari selama 14 hari. Saat sebelum dan sesudah perlakuan selama 14 hari, darah diambil melalui chantus medial orbitalis untuk pemeriksaan pretest dan postest dan kadar testosteron diperiksa dengan metode ELISA. Hasil penelitian menunjukkan bahwa sebelum perlakuan (pretest), rerata kadar testosteron kelompok P0 8,29±4,68 nmol/L, sedangkan pada kelompok P1 7,39±1,45 nmol/L (P > 0,05). Setelah perlakuan selama 14 hari, rerata kadar testosteron kelompok P0 ialah 7,40±1,37 nmol/L dan pada kelompok P1 3,36±1,26 nmol/L (P < 0,01). Analisis efek perlakuan menunjukkan tidak terjadi penurunan kadar hormon testosteron pada kelompok P0 (P > 0,05), sedangkan penurunan bermakna terdapat pada kelompok P1 (P < 0,01). Simpulan: Pemberian bisphenol A (BPA) oral dapat menurunkan kadar testosteron pada tikus (Rattus norvegicus) jantan galur Sprague Dawley.Kata kunci: bisphenol A (BPA), testosteron

scholarly journals Pemberian suplemen Swissoats A3® meningkatkan kadar hormon estrogen pada tikus (Rattus norvegicus) betina menopause galur Wistar

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Trisna A. Widhayanthi ◽  
Wimpie Pangkahila ◽  
Gde Ngurah Indraguna Pinatih
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Indirect Elisa ◽  
Control Group ◽  
Medial Canthus ◽  
Control Group Design ◽  
Group Design ◽  
Estrogen Level ◽  
Female Wistar Rats ◽  
The Mean

Abstract: Aging is a physiological process that occurs in all living creatures. Swissoats A3® is an anti-aging supplement that contains phytoestrogens. This study was aimed to prove that Swiss oats A3® supplementation could increase estrogen levels in menopausal female Wistar rats (Rattus norvegicus). This was an experimental study with a completely randomized pretest-posttest control group design. Subjects were 20 menopausal female rats (Rattus norvegicus), Wistar strain, healthy, aged 15-16 months, weighing 200 g, divided into 2 groups: the control group (P0), treated with distilled water for 21 days and the treatment group (P1), treated with Swissoats A3® of 34.2 mg/rat/day for 21 days. Before and after 21 days of treatment, 1 ml venous blood was drawn through the medial canthus of orbital sinus using microcapillary tube and the estrogen levels were meassured by using indirect ELISA method. The results showed that before treatment, the mean estrogen level in P0 group was 14.96±2.0468 pg/ml and in P1 group was 14.21±2.3905 pg/ml (P > 0.05). Meanwhile, after 21-day treatment, the mean estrogen level in P0 group was 15.07±1.8851 pg/ml, whereas in P1 group was 28.33±3.2052 pg/ml (P < 0.01). The analysis showed that the Swissoats A3® could elevate the estrogen levels significantly (P < 0.01) in group P1 (2.3905±14.21 vs 28.33±3.205). Conclusion: Swissoats A3® supplementation could increase the estrogen levels in menopausal female Wistar rats (Rattus norvegicus).Keywords: Swiss oats A3®, estrogen, female Wistar rats, menopauseAbstrak: Penuaan (aging) merupakan suatu proses fisiologik yang dialami oleh seluruh mahluk hidup. Swissoats A3® merupakan suplemen anti-aging yang mengandung fitoestrogen. Penelitian ini bertujuan untuk membuktikan bahwa pemberian suplemen Swissoats A3® dapat meningkatkan kadar estrogen pada tikus (Rattus norvegicus) betina menopause galur Wistar. Jenis penelitian ialah eksperimental dengan randomized pretest-posttest control group design. Subjek penelitian ialah 20 ekor tikus putih betina (Rattus norvegicus) galur Wistar, sehat, menopause, umur 15-16 bulan, berat badan 200 gr. Tikus dibagi menjadi 2 kelompok yaitu kelompok kontrol (P0) yang diberikan aquadest secara sonde selama 21 hari dan kelompok perlakuan yang diberikan suplemen Swissoats A3® dosis 34,2mg/ekor/hari secara sonde selama 21 hari. Sebelum dan sesudah 21 hari perlakuan, darah vena diambil melalui medial canthus sinus orbitalis sekitar 1 ml dengan menggunakan tabung mikrokapiler dan dilakukan pemeriksaan kadar estrogen menggunakan metode indirect ELISA. Hasil penelitian menunjukkan bahwa sebelum perlakuan, rerata kadar estrogen kelompok P0 14,96±2,0468 pg/ml dan kelompok P1 14,21±2,3905 pg/ml (P > 0,05). Setelah perlakuan selama 21 hari, rerata kadar estrogen kelompok P0 15,07±1,8851 pg/ml dan kelompok P1 28,33±3,2052 pg/ml (P < 0,01). Analisis efek perlakuan menunjukkan terjadinya peningkatan kadar hormon estrogen yang sangat bermakna (P < 0,01) pada kelompok P1 (14,21±2,3905 vs 28,33±3,205). Simpulan: Swissoats A3® dapat meningkatkan kadar estrogen pada tikus (Rattus norvegicus) betina menopause galur wistar.Kata kunci: Swissoats A3®, estrogen, tikus wistar betina, menopause

scholarly journals Pengaruh Susu Kedelai terhadap Ekspresi Reseptor Estrogen-Β Uterus pada Masa Reproduksi Awal Rattus Norvegicus Betina

2019 ◽  
Vol 5 (1) ◽  
pp. 17
Author(s):  
Vide Bahtera Dinastiti ◽  
Nurdiana Nurdiana ◽  
Pande Made Dwijayasa
Keyword(s):  
Rattus Norvegicus ◽  
Endocrine System ◽  
Reproductive Function ◽  
Potential Effect ◽  
Reproductive Period ◽  
The Body ◽  
Control Group ◽  
P Value ◽  
Soy Milk ◽  
Control Group Design

 Receptors estrogen can also stimulated by other, namely by fitoestrogen. Fitoestrogen are the group of non steroid plants that have composition resembling the natural estrogen in the body. One of it is isoflavon, that can be binding with receptor estrogen, which affinity for RE-β is greater than RE-α and can make potential effect because receptors estrogen will blocked by isoflavon and cannot be occupied by estrogen.This compound can harm the endocrine system of the body because of the possible bad effects for reproductive function. Isoflavonoid is mostly found in soy. Soybean can be processed to soybean milk. The purpose of this study is demonstrate that administration of soy milk can affect the  expression of RE-β uterus at the early reproductive period of female Rattus norvegicus. The method used is an experimental design post test control group design. The sample in this study is 24 Rattus norvegicus females. Then divided into four groups: one control group and three treatment groups were given soy milk each dose of 2.5 mg/kg/weight; 5mg/kg/weight; 10mg/kg/weight. The  expression of RE-β uterus were measured by immunohistochemistry. Based on the analysis using ANOVA, was obtained p-value of 0.000, smaller than α = 0:05 (p <0.05). There is a significant influence on the administration of soy milk increase expression of RE-β and by LSD test obtained the results of differences from application of soy milk with different doses. 

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