FORMULASI DAN UJI EFEKTIVITAS ANTIBAKTERI SEDIAAN GEL EKSTRAK  ETANOL DAUN KERSEN (Muntingia calabura L.) SEBAGAI ANTISEPTIK TANGAN

Kerson Fruit (Muntingia calabura L.) contains bioactive compounds, such as flavonoids, saponins, triterpenes, steroids, and tannins, which are potentially as antibacterial compounds. The aim of this study was to make a formulation of hand antiseptic gel from kerson leaf extracts with three variations in extract concentrations of 5%, 10%, and 15%, and to test the effectiveness of antibacterial preparations against Staphylococcus aureus bacteria. The method of this research is laboratory experimental research. Kerson extracts was obtained by maceration using 96% ethanol. The results showed that kerson leaf extract can be formulated as a hand antiseptic gel preparation that meets organoleptic requirements, like homogeneity, pH, dispersion, consistency, adhesion, cycling test, and antiseptic power testing using a colony counter. On the results of antibacterial effectiveness testing, there is a clear zone that presents the ability to inhibit the growth of gel test bacteria. The average diameter of the hand antiseptic gel preparation of kersen leaf extract at concentrations of 5%, 10%, and 15% respectively were 10.00 mm, 11.66 mm and 12.00 mm so that the ability of inhibition of gel test bacteria in all concentrations was categorized strong. Keywords: Kerson, Muntingia calabura L, Hand Antiseptic Gel, Staphylococcus aureus. ABSTRAK Kersen (Muntigia calabura L.) mengandung senyawa bioaktif yaitu senyawa flavonoid, saponin, triterpen, steroid, dan tannin yang merupakan senyawa yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk membuat formulasi sediaan gel antiseptik tangan dari ekstrak daun kersen dengan tiga variasi konsentrasi ekstrak yakni 5%, 10%, dan 15%, serta menguji efektivitas antibakteri sediaan terhadap bakteri Staphylococcus aureus. Metode penelitian ini merupakan penelitian eksperimental laboratorium. Ekstrak tanaman kersen diperoleh dengan cara maserasi menggunakan pelarut etanol 96%. Hasil penelitian menunjukkan bahwa ekstrak daun kersen dapat diformulasikan sebagai sediaan gel antiseptik tangan yang memenuhi persyaratan organoleptik, homogenitas, pH, daya sebar, konsistensi, daya lekat, cycling test, dan pengujian daya antiseptik dengan menggunakan alat colony counter. Pada hasil pengujian efektivitas antibakteri, terdapat zona bening yang mempresentasikan kemampuan penghambatan pertumbuhan bakteri uji oleh gel. Diameter rata-rata sediaan gel antiseptik tangan ekstrak daun kersen pada konsentrasi 5%, 10%, dan 15% berturut-turut yaitu 10,00 mm , 11,66 mm dan 12,00 mm sehingga kemampuan penghambatan bakteri uji oleh gel disemua konsentrasi dikategorikan kuat. Kata kunci: Kersen, Muntingia calabura L, Gel Antiseptik Tangan, Staphylococcus aureus.

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Effectiveness of Chinese Leaf Extract and Leaf Plants (Jatropha Multifida L.) As Antibacterial to Bacterial Growth Staphylococcus Aureus In Vitro

Window of Health Jurnal Kesehatan ◽

10.33368/woh.v0i0.13 ◽

2018 ◽

pp. 29-33

Author(s):

Dwi Anggita ◽

Yusriani Yusriani ◽

Dian Amelia Abdi ◽

Vivin Desiani

Keyword(s):

Staphylococcus Aureus ◽

Leaf Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Positive Control ◽

Diffusion Method ◽

Leaf Extracts ◽

The Difference ◽

Jatropha Multifida

Jatropha multifida L. contains alkaloids, tannins, flavonoids, saponins and phenolic acids that differ from each part of the plant and the content of these substances is what makes L. multifida Jatropha has a function as an antibacterial so that the leaf extract and the gap of chinese distance ( Jatropha multifida L.) allegedly capable of inhibiting the growth of Staphylococcus aureus bacteria. The aim of this research was to know the effectivity of leaf extract and gum of Jatropha multifida L. to the growth of Staphylococcus aureus bacteria in vitro.Penelitian used true experimental post test design. Using disc diffusion method with concentration 25; 50; 75; and 100% v / v leaf and gum china distance. Positive control was used Clindamycin 5μg drip antibiotic paper disc. The data were analyzed descriptively. The result of the research showed the difference of different inhibition zone between treatments. In chinese leaf extracts showed different mean diameters, at concentrations of 25%, 50%, 75% with inhibit zone formed respectively 0 mm, 9.32 mm, and 17.48 mm and the highest inhibition zone at concentration of 100% with an average diameter of 22.24 mm. In the gap of chinese distance showed a different mean diameter, at concentrations of 25%, 50%, 75% with the inhibit zone formed respectively 16.08 mm, 18.15 mm, and 18.63 mm and the highest inhibition zone at a concentration of 100% with an average diameter of 21.91 mm. Clindamycin positive controls show an average inhibit zone of 23.31 mm. It was concluded that there was an effect of leaf extract and resin of chinese distance plant (Jatropha multifida L.) on growth of Staphylococcus aureus bacteria, and the best concentration was 100% concentration and almost closer to the inhibitory zone of Clindamycin antibiotics as positive control.

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Formulasi Sediaan Sabun Padat Ekstrak Daun Lamun (Thalassia hemprichii)

WARTA FARMASI ◽

10.46356/wfarmasi.v8i2.96 ◽

2019 ◽

Vol 8 (2) ◽

pp. 53-62

Author(s):

Nirwati Rusli ◽

Eny Nurhikma ◽

Elma Puspita Sari

Keyword(s):

Staphylococcus Aureus ◽

Leaf Extract ◽

Room Temperature ◽

Thalassia Hemprichii ◽

Leaf Extracts ◽

Cycling Test ◽

Physical Evaluation ◽

Time Space ◽

Solid Form ◽

Made In

Lamun (Thalassia hemprichii) merupakan tanaman utama dimanfaatkan sebagai bahan kecantikan, obat, dan bidang farmasi. yang memiliki kandungan alkaloid, flavonoid tanin dan fenol. yang dapat menghambat pertumbuhan bakteri Staphylococcus aureus. Aktifitas ini lebih efektif dibuat dalam sediaan sabun. Sabunyang dapat membunuh bakteri dikenal dengan sabun antiseptik. dengan menggunakan ekstrak daun lamun. Tujuan dari penelitian ini yaitu untuk mengetahui apakah ekstrak daun lamun dapat di formulasikan sabun mandi padat dan Untuk mengetahui konsentrasi dari ekstrak daun lamun (Thalassiahemprichi) yang memenuhi syarat evaluasi fisik dan uji stabilitas sediaan sabun padat. Jenis penelitian yang dilakukan adalah eksperimen dengan membuat sediaan sabun padat. Daun lamun di ekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Ekstrak yang diperoleh kemudian dibuat sediaan sabun padat dengan konsentrasi ekstrak daun lamun sebesar 15% (FA), 25% (FB), 35% (FC). Formulasi di uji secara evaluasi fisik pada suhu kamar dan cycling test. Evaluasi sediaan meliputi organoleptik, pH sediaan, homogenitas, cycling test dan waktu ruang. Hasil evaluasi fisik sediaan sabun padat ekstrak daun lamun yang dilakukan selama 4 minggu menunjukkan ketiga formula yang dibuat memenuhi syarat evaluasi fisik sediaan,yaitu formula C karena memiliki bentukya padat. Kata kunci : Ekstrak daun lamun, Sabun Padat, evaluasi fisik dan uji stabiilitas sediaan. ABSTRACT Seagrass (Thalassia hemprichii) is the main plant used as a beauty ingredient, medicine, and pharmaceutical field. which contains alkaloids, tannin flavonoids and phenols. which can inhibit the growth of Staphylococcus aureus bacteria. This activity is more effectively made in soap preparations. Soaps that can kill bacteria are known as antiseptic soaps. by using seagrass leaf extract. The purpose of this study is to find out whether seagrass leaf extracts can be formulated in solid soap and to determine the concentration of seagrass leaf extracts (Thalassia hemprichi) that meet the physical evaluation and stability tests of solid soap preparations. The type of research conducted was an experiment by making solid soap preparations. Seagrass leaves were extracted by maceration method using 96% ethanol solvent. The extract obtained was then made into a solid soap preparation with a concentration of seagrass extract at 15% (FA), 25% (FB), 35% (FC). The formulation was tested by physical evaluation at room temperature and by cycling test. Evaluation of preparations includes organoleptic, dosage pH, homogeneity, cycling tests and time space. The results of physical evaluation of seagrass leaf extract solid soap which was carried out for 4 weeks showed that the three formulas that were made met the physical evaluation requirements of the preparation, namely formula C because it has a solid form. Keywords: Seagrass leaves extract, Solid Soap, physical evaluation and stabiility test preparations.

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FORMULASI DAN UJI EFEKTIVITAS KRIM ANTIBAKTERI EKSTRAK ETANOL DAUN BELUNTAS (Pluchea indica Less.) TERHADAP BAKTERI Propionibacterium acnes

PHARMACON ◽

10.35799/pha.8.2019.29256 ◽

2019 ◽

Vol 8 (1) ◽

pp. 214

Author(s):

Eunike Suru ◽

Paulina V. Y. Yamlean ◽

Widya A Lolo

Keyword(s):

Antibacterial Activity ◽

Leaf Extract ◽

Propionibacterium Acnes ◽

Physical Stability ◽

Average Diameter ◽

Laboratory Research ◽

Clear Zone ◽

Cycling Test ◽

Experimental Laboratory

ABSTRACT Beluntas plants contain flavonoid compounds, which are know to have antibacterial activity. This study aims to make a formulate and test the physical stability of antibacterial cream prepared from Beluntas Leaf extract (Pluchea indica Less.) with three variations in extract concentrations of 5%, 10% and 15%, and test the effectiveness of antibacterial against Propionibacterium acnes bacteria. The method of this research is experimental laboratory research. Beluntas leaves extract (Pluchea indica Less.) was obtained by maceration using 96% of ethanol and formulated into cream preparations with various extract concentrations. The result showed that Beluntas Leaf extract cream fulfilled the requirements of the physical properties of cream preparations, which included organoleptic test, homogeneity, pH, dispersion, adhesion, emulsion type and cycling test. On the result of antibacterial activity testing, the average diameter of the clear zone of prepared cream of Beluntas leaf extract was at concentration of 5%, 10% and 15%, respectively ie 6.16 mm, 7.83 mm, 10.16 mm. Based on the classification of antibacterial category, the inhibiting ability of the test bacteria by 5% and 10% cream was categorized as moderate, and 15% was categorized as strongly which is the most effective cream inhibiting the activity of Propionibacterium acnes. Keywords: Beluntas leaves, antibacterial cream, Propionibacterium acnes ABSTRAK Tanaman Beluntas merupakan salah satu tanaman yang berkhasiat obat. Tanaman Beluntas mengandung senyawa flavonoid yang diketahui memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk membuat formula dan uji stabilitas fisik sediaan krim antibakteri dari ekstrak Daun Beluntas (Pluchea indica Less.) dengan tiga variasi konsentrasi ekstrak yaitu 5%, 10% dan 15%, serta menguji efektivitas antibakteri terhadap bakteri Propionibacterium acnes. Metode penelitian ini merupakan penelitian eksperimental laboratorium. Ekstrak daun Beluntas (Pluchea indica Less.) diperoleh dengan cara maserasi dengan pelarut etanol 96% dan diformulasikan menjadi sediaan krim dengan variasi konsetrasi ekstrak. Hasil penelitian menunjukkan bahwa krim ekstrak Daun Beluntas memenuhi persyaratan sifat fisik sediaan krim yang meliputi uji organoleptik, homogenitas, pH, daya sebar, daya lekat, tipe emulsi dan cycling test .Pada hasil pengujian aktivitas antibakteri, diameter rata-rata zona bening sediaan krim ekstrak daun beluntas pada konsetrasi 5%, 10% dan 15% berturut –turut yaitu 6,16 mm, 7,83 mm, 10,16 mm. Berdasarkan klasifikasi kekuatan daya antibakteri, maka kemampuan penghambatan bakteri uji oleh krim konsentrasi 5% dan 10% dikategorikan sedang, serta 15% dikategorikan kuat yang merupakan krim paling efektif menghambat aktivitas bakteri Propionibacterium acnes. Kata kunci : Daun Beluntas, krim antibakteri, Propionibacterium acnes.

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FORMULASI SEDIAAN KRIM KOMBINASI EKSTRAK DAUN SELEDRI (Apium graveolens L.) DAN DAUN JATI (Tectona grandis Linn.F) SEBAGAI ANTIBAKTERI TERHADAP Staphylococcus aureus

PHARMACON ◽

10.35799/pha.9.2020.29280 ◽

2020 ◽

Vol 9 (2) ◽

pp. 259

Author(s):

Riska Tuloli ◽

Hosea Jaya Edi ◽

Imam Jayanto

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Leaf Extract ◽

Tectona Grandis ◽

Test Method ◽

Apium Graveolens ◽

Homogeneity Test ◽

Cycling Test ◽

Physical Tests ◽

Dispersion Test

ABSTRACT Celery plants (Apium graveolens L.) and teak plants (Tectona grandis Linn.f) are plants that contain flavonoids, tannins, and saponins and has antibacterial activity against Staphylococcus aureus. This study aims to test the antibacterial effectiveness of combination of celery and teak leaf cream and evaluate cream with parameters of physical tests (organoleptic, homogeneity, pH, spreadability, adhesion) and physical stability with cycling test method. This study used an experimental laboratory method by making 5 variations concentration of extracts. Celery and teak leaf extract was extracted by maceration method. Antibacterial test used the wells method. Results of the evaluation of physical tests for organoleptic test (odor: a typical combination of extracts;color: brown;shape: semi-solid), homogeneity test (homogeneous), average of pH test is 6.02, dispersion test is 4.27 cm, adhesion test is 18.42 seconds. Based on the results of this study, concluded that the combination of celery leaf and teak leaf extract can be formulated into a cream. The cream of combination of celery and teak leafs extract are bacteriostatic and has antibacterial activity with an average diameter for formulation I 13.4 mm, formulation II 9.23 mm, formulation III 16.23 mm, formulation IV 11 mm, and formulation V 12.06 mm. Keywords : Celery (Apium graveolens L.), Teak (Tectona grandis Linn.f), Antibacterial, Cream. ABSTRAK Tanaman seledri (Apium graveolens L.) dan tanaman jati (Tectona grandis Linn.f) merupakan tanaman yang mengandung flavonoid, tanin dan saponin dan memiliki aktivitas antibakteri terhadap Staphylococcus aureus. Penelitian ini bertujuan untuk menguji aktivitas antibakteri sediaan krim kombinasi ekstrak daun seledri dan daun jati serta mengevaluasi sediaan krim dengan parameter uji sifat fisik (uji organoleptik, uji homogenitas, uji pH, uji daya sebar, uji daya lekat) dan stabilitas fisik dengan metode cycling test. Penelitian ini menggunakan metode eksperimental laboratorium dengan membuat 5 variasi konsentrasi ekstrak. Ekstrak diperoleh dengan cara maserasi. Uji antibakteri dilakukan dengan metode sumuran. Hasil evaluasi sifat fisik untuk uji organoleptik (bau: khas kombinasi ekstrak;warna:coklat;bentuk:setengah padat), uji homogenitas (susunan homogen), rata-rata uji pH 6,02, daya sebar 4,27 cm, daya lekat 18,42 detik. Berdasarkan hasil penelitian disimpulkan bahwa kombinasi ekstrak daun seledri dan daun jati dapat diformulasikan menjadi sediaan krim. Sediaan krim kombinasi ekstrak daun seledri dan daun jati bersifat bakteriostatik dan memiliki aktivitas antibakteri dengan diameter rata-rata untuk formula I 13,4 mm, formula II 9,23 mm, formula III 16,23 mm, formula IV 11 mm, dan formula V 12,06 mm. Kata Kunci : Seledri (Apium graveolens L.), Jati (Tectona grandis Linn.f), Antibakteri, Krim

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Potential of Malacca leaf (Phyllanthus emblica) against Salmonella sp

E3S Web of Conferences ◽

10.1051/e3sconf/202015101029 ◽

2020 ◽

Vol 151 ◽

pp. 01029

Author(s):

Nuzul Asmilia ◽

Mahdi Abrar ◽

Yudha Fahrimal ◽

Amalia Sutriana ◽

Yobeswi Husna

Keyword(s):

Ethyl Acetate ◽

Leaf Extract ◽

Ethyl Acetate Extract ◽

Ethanol Extract ◽

Average Diameter ◽

Positive Control ◽

Hexane Extract ◽

Clear Zone ◽

Inhibitory Property

Malacca is one of traditional medicine that possesses a potent antimicrobial activity. This study aims to determine the inhibitory activity of Malacca leaf extract on the growth of Salmonella sp in vitro. The bacteria was obtained from Microbiology Laboratory of the Faculty of Veterinary Medicine, Universitas Syiah Kuala. The study was conducted using n-hexane extract, ethyl acetate extract and ethanol of malacca leaves with dilution concentrations of 5%, 25%, and 50%.The inhibitory property of malacca leaf was tested using Kirby-Bauer method. Data were analyzed descriptively. The results of this study indicate that n-hexane extract, ethyl acetate extract and ethanol extract of malacca leaves can inhibit the growth of Salmonella sp. The n-hexane extract of malacca leaves showed a greater inhibition than the ethyl acetate and ethanol extract of malacca leaves. n-hexane extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 1.35 mm (weak), 4.97 mm (moderate), and 12.87 mm (strong), respectively ethyl acetate extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 2.00 mm (weak), 5.72 mm (moderate), and 7.58 mm (moderate), whereas in ethanol extract were 0.47 mm (weak), 2.58 mm (weak), and 4.35 mm (weak), repectively. The clear zone areas in negative and positive control were 0.00 mm 20.00 mm, respectively. Malacca leaf extract possess inhibitory property against the growth of the Salmonella sp.

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EFEKTIVITAS GEL EKSTRAK ETANOL BAWANG PUTIH (Allium sativum L.) TERHADAP BAKTERI Staphylococcus aureus

Jurnal Riset Kefarmasian Indonesia ◽

10.33759/jrki.v2i2.84 ◽

2020 ◽

Vol 2 (2) ◽

pp. 105-112

Author(s):

Irene Puspa Dewi ◽

Ilham Maslan Orde ◽

Verawaty Verawaty

Keyword(s):

Staphylococcus Aureus ◽

Allium Sativum ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Traditional Medicines ◽

Significant Difference ◽

Irritation Test ◽

Gel Preparation ◽

The One

Garlic (Allium sativum L.) is one of the traditional medicines which has an antibacterial efficacy compound namely Allisin which is able to inhibit the growth of Staphylococcus aureus bacteria. This study aims to formulate the garlic bulb extract into a good gel preparation for use as an acne drug and to determine the antibacterial effectiveness of the ethanol extract of garlic gel against the bacterium Staphylococcus aureus using the wells method. The gel evaluation was carried out to meet the requirements with organoleptic test parameters, homogeneity, pH, spreadability, adhesion and irritation test. This study uses extracts of 10% and 20% concentrations, at each concentration being able to inhibit the growth of Staphylococcus aureus bacteria. In the formula, the concentration of 20% has a inhibition zone diameter of 1.59 cm and a concentration of 10% has a inhibition of 1.50 cm. Statistical test results using the One Way ANOVA test found that there was no significant difference between the average diameter of inhibition of garlic extract gel concentration of 10% with a concentration of 20%.

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Antimicrobial Activity of Azadirachta indica, against Target Pathogens and Its Utility as a Disinfectant and Floor Cleaner

Journal of Evolution of Medical and Dental Sciences ◽

10.14260/jemds/2021/392 ◽

2021 ◽

Vol 10 (25) ◽

pp. 1899-1903

Author(s):

Mohsin Ali Khan ◽

Shadma Yaqoob ◽

Sharique Ahmad

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Azadirachta Indica ◽

Leaf Extract ◽

Diffusion Method ◽

Leaf Extracts ◽

Neem Extract ◽

Inoculum Preparation ◽

Neem Leaf Extract ◽

Agar Well Diffusion Method

BACKGROUND Azadirachta indica, commonly known as neem, neem tree or Indian lilac, Limbo, Nim, Nimba, Medusa and Vempu. It is typically grown in tropical and subtropical regions. Neem belongs to family meliaceae. Neem is a native tree of India. It is also called “village pharmacy of south Asia” (India) because of its enormous medicinal properties and people use it most of the time without knowing its multiple advantages. This study was done to know the efficacy of medicinal plant named Azadirachta indica (Neem) against pathogenic microorganisms and about its utility as disinfectant and floor cleaner. METHODS Agar well diffusion method, Culture plate method, inoculum preparation & its subculture methods were used to determine the antimicrobial activity of the neem leaf extract. Different concentrations of neem leaf extracts in culture plates were used for the study. In this method inhibited zones were measured. RESULTS Neem leaf extracts showed considerable antimicrobial activity against four target pathogens. In agar well diffusion method it showed maximum antimicrobial activity against Enterococcus and Staphylococcus aureus. Growth of inhibition was increased as the concentration of the neem extract in agar media increased and was maximum for Staphylococcus aureus. CONCLUSIONS The extract of A. indica, has antimicrobial activity against target pathogens Enterococcus, Staphylococcus aureus, Pseudomonas, and E.coli. With the old medicinal knowledge on neem, better economic and therapeutic utilization can be done by using modern approaches of drug development. KEY WORDS Neem Leaf extract, Antimicrobial Activity, Agar Well Diffusion Test, Zone of Inhibition

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Konsentrasi Hambatan Minimum ekstrak Portulaca grandiflora terhadap penyakit Motile Aeromonad Septicaemia

e-Journal BUDIDAYA PERAIRAN ◽

10.35800/bdp.6.2.2018.20659 ◽

2018 ◽

Vol 6 (2) ◽

Author(s):

Dafid Lauluw ◽

Reni L. Kreckhoff ◽

Sammy N.J. Longdong ◽

Desy M. Mantiri ◽

Juliaan Ch. Watung ◽

...

Keyword(s):

Leaf Extract ◽

Agar Medium ◽

Average Diameter ◽

Inhibition Zone ◽

Clear Zone ◽

Minimum Concentration ◽

Portulaca Grandiflora ◽

Standard Classification ◽

Classification Table ◽

Paper Disc

The objective of this study was to determine the minimum concentration of extract in inhibiting motile motile aeromonad septicaemia. The material used was the stems and leaves of the plant P. grandiflora as much as 10 g. The crushed simplicia was then macerated with aquades with a ratio of 1:10, then filtered with filter paper and, put in an Erlenmeyer, and evaporated in an incubator at temperature of 30°C. The sensitivity test was performed by placing a paper disc containing extract on the agar medium that had been scratched with bacterial colonies. Clear zone formed around the paper disc was compared with standard classification table for clarifying the inhibition level. The results showed that stem and leaf extract had inhibition zone against Aeromonas hydrophila bacteria that caused motile aeromonad septicaemia. The best result was obtain at concentration of extract 3.25 mg / L) with an average diameter of 22.67 mm and for leaf extract had a moderate to strong zone for all treatments. These proved that stem and leaf extract of P. grandiflora plant had antibacterial activity and can be used as control of MAS disease caused by A. hydrophila bacteria. Keywords: Portulaca grandiflora plant, motile aeromonad septicemia, minimum inbibition concentration

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FORMULASI SEDIAAN GEL EKSTRAK ETANOL DAUN KALIANDRA (Calliandra surinamensis Benth) SEBAGAI ANTIBAKTERI

PHARMACON ◽

10.35799/pha.8.2019.29356 ◽

2019 ◽

Vol 8 (4) ◽

pp. 801

Author(s):

Yulita Ni Nyoman Tri Sukartiningsih ◽

Hosea Jaya Edi ◽

Jainer P. Siampa

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Physical Stability ◽

Homogeneity Test ◽

Adhesion Test ◽

Cycling Test ◽

Physical Evaluation ◽

Before And After ◽

Gel Preparation ◽

Ethanol Extracts

ABSTRACT Kaliandra Leaf (Calliandra surinamensis Benth) contains flavonoid compounds, saponins and tannins which are able to inhibit the antibacterial activity. This study aims to test the antibacterial effectiveness of gel preparation of ethanol extracts of kaliandra leaf to obtain the greatest formula, and prove the Kaliandra leaf gel preparation has good physical stability. This study uses a laboratory experimental method. Gel formula is made as many as 5 formulas with variations in the concentration of 2%, 3%, 4%, 5%, and 6% of w : v. Kaliandra leaf extraction was carried out by maceration method using 96% ethanol. The antibacterial test of the ethanol extracts of Kaliandra leaf gel using the method of welling against Staphylococcus aureus bacteria produced a moderate inhibition of 7.0 ± 0.63 mm at a concentration of 6%. The physical evaluation of the preparation includes organoleptic test, homogeneity test, syneresis test, scatter power test, adhesion test, pH test, cycling test, and sterility test. All tests are carried out before and after the cycling test. The results of the study on the preparation fulfilled the physical evaluation requirements before and after the cycling test. It can be concluded that the concentration of 6% of ethanol extracts of Kaliandra leaves could be formulated as a gel preparation that is physically stable and has moderate antibacterial activity. Keywords: Kaliandra (Calliandra surinamensis Benth), Gel preparations, Staphylococcus aureus antibacterial. ABSTRAK Daun Kaliandra (Calliandra surinamensus Benth) mengandung senyawa flavonoid, saponin dan tannin yang mampu menghambat aktivitas antibakteri. Penelitian ini bertujuan untuk menguji efektivitas antibakteri sediaan gel ekstrak etanol daun Kaliandra untuk mendapatkan formula terbesar, serta membuktikan sediaan gel daun Kaliandra mempunyai stabilitas fisik yang baik. Penelitian ini menggunakan metode ekperimental laboratorium. Formula sediaan gel dibuat sebanyak 5 formula dengan variasi konsentrasi 2%, 3%, 4%, 5%, dan 6% b/v. Ekstraksi daun Kaliandra dilakukan dengan metode maserasi menggunakan etanol 96%. Penelitian uji antibakteri sediaan gel ekstrak etanol daun Kaliandra menggunakan metode sumuran pada bakteri staphylococcus aureus menghasilkan daya hambat yang sedang yaitu 7,0±0,63 mm pada konsentrasi 6%. Evaluasi fisik sediaan meliputi uji organoleptik, uji homogenitas, uji sineresis, uji daya sebar, uji daya lekat, uji pH, uji cycling test, dan uji sterilitas. Semua pengujian dilakukan sebelum dan sesudah cycling test. Hasil penelitian sediaan memenuhi persyaratan eveluasi fisik sebelum uji cycling test dan sesudah cycling test. Dapat disimpulkan pada kosentrasi 6% ekstrak etanol daun Kaliandra dapat diformulasi sebagai sediaan gel yang stabil secara fisik dan memiliki aktivitas antibakteri yang sedang. Kata kunci : Kaliandra (Calliandra surinamensis Benth), Sediaan Gel, Antibakteri Staphlococcous aureus.

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UJI BIOAKTIVITAS EKSTRAK DAUN BIDARA BIDARA (ZIZIPHUS MAURITIANA LAM) TERHADAP PERTUMBUHAN STAPHYLOCOCCUS AUREUS

Jurnal Kesehatan Panrita Husada ◽

10.37362/jkph.v4i1.181 ◽

2019 ◽

Vol 4 (1) ◽

pp. 49-57

Author(s):

Aan Yulianingsih ◽

Dzikra Arwie

Keyword(s):

Staphylococcus Aureus ◽

Leaf Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Ziziphus Mauritiana ◽

Diffusion Test ◽

Normal Microflora ◽

Inhibitory Power

The background of this study is Staphylococcus aureus is an anaerobic facultative bacterium and one of the normal microflora in the mouth. However, if it is influenced by predisposing factors, it will become a pathogen. Bidara leaves have antimicrobial activity against Staphylococcus aureus, because Bidara leaves contain various compounds including alkaloids, flavonoids, steroids, tannins and essential oils. The purpose of this study was to determine how much inhibitory power produced by Bidara leaf extract on the growth of Staphylococcus aureus bacteria. This research method is a laboratory experimental research using the diffusion test (wells) method. The sample of this study was Staphylococcus aureus. Dilution of Bidara leaf extract consists of 5 concentrations including: 20%, 40%, 60%, 80%, and 100%. Chlorampheniocol is used for Positive control while Negative control uses aquadest. Based on observations show that the average diameter of inhibitory zone Bidara leaf extract at a concentration of 20% with a diameter of 10.5 mm (R), at a concentration of 60% and 80% with a diameter of 16 mm and 18 mm (I), at a concentration of 100% with a diameter of 24 mm (S). It can be concluded that the higher the concentration of Bidara leaf extract, the inhibition zone formed will increase. So Bidara leaves are good for use in treating infections caused by Staphylococcus aureus bacteria.

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