EFEKTIVITAS GEL EKSTRAK ETANOL BAWANG PUTIH  (Allium sativum L.) TERHADAP BAKTERI Staphylococcus aureus

Garlic (Allium sativum L.) is one of the traditional medicines which has an antibacterial efficacy compound namely Allisin which is able to inhibit the growth of Staphylococcus aureus bacteria. This study aims to formulate the garlic bulb extract into a good gel preparation for use as an acne drug and to determine the antibacterial effectiveness of the ethanol extract of garlic gel against the bacterium Staphylococcus aureus using the wells method. The gel evaluation was carried out to meet the requirements with organoleptic test parameters, homogeneity, pH, spreadability, adhesion and irritation test. This study uses extracts of 10% and 20% concentrations, at each concentration being able to inhibit the growth of Staphylococcus aureus bacteria. In the formula, the concentration of 20% has a inhibition zone diameter of 1.59 cm and a concentration of 10% has a inhibition of 1.50 cm. Statistical test results using the One Way ANOVA test found that there was no significant difference between the average diameter of inhibition of garlic extract gel concentration of 10% with a concentration of 20%.

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OPTIMASI HPMC DAN KARBOPOL DALAM FORMULASI SEDIAAN GEL ANTISEPTIK EKSTRAK ETANOL DAUN BELUNTAS DAN AKTIVITAS ANTIBAKTERI TERHADAP Staphylococcus aureus

Jurnal Insan Farmasi Indonesia ◽

10.36387/jifi.v3i2.566 ◽

2020 ◽

Vol 3 (2) ◽

pp. 327-336

Author(s):

Muhammad Dienulloh Qasyfur Rohman ◽

◽

Iwan Setiawan ◽

Ardy Prian Nirwana

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Design Method ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Lattice Design ◽

Simplex Lattice ◽

Gel Preparation ◽

Optimum Formula

Beluntas leaf (Pluchea indica, L.) is one of the plants that has potential as an antibacterial, Where the leaves of Beluntas have several compounds, one of these is a flavonoid which has antibacterial activity. The aim of this research is to determine the optimum composition of HPMC and carbopol which will be formulated in the preparation of antiseptic gel ethanol extract of beluntas leaf using the SLD method. The extraction method used in this study is Maseration with ethanol as a solvent. Antiseptic gel manufacturing is done by optimizing the base of HPMC and Carbopol, Then testing the quality of the gel preparation and the results obtained that meet the requirements of the homogeneity, pH, dispersion, viscosity, consistency and stability test of the gel preparation. The optimum formula of antiseptic gel based on the simplex lattice design method is on a combination of a 4.5% HPMC base and a 0.5% carbopol. Antibacterial activity test found inhibition zone, this shows that the antiseptic gel preparation of the ethanol extract of beluntas leaves has the ability to inhibit against Staphylococcus aureus bacteria with an average diameter of the zone of inhibition by 17,66 mm.

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DAYA HAMBAT DAUN MANUKAN (Rhinacanthus nasutus (L) Kurz) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS SECARA IN VITRO

BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) ◽

10.31289/biolink.v6i2.2731 ◽

2019 ◽

Vol 6 (2) ◽

pp. 106-113

Author(s):

Sunarti Sunarti ◽

Debora Paninsari

Keyword(s):

Staphylococcus Aureus ◽

Pathogenic Bacteria ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibition Zone ◽

In Vitro Test ◽

Extract Concentration ◽

Significant Difference ◽

Rhinacanthus Nasutus

The use of antimicrobial such as antibiotics against infectious diseases with no control is quite common in Indonesia. This leads to increase morbidity and incidence of drug resistance. Staphylococcus aureus is a pathogenic bacteria, particularly infects the skin. The resistance it difficult to determine which antimicrobials were suitable for therapy. Manukan leaves have an active substance Rhinacanthin can be used as an anti-bacterial. The aim of this study was to determine the inhibition of ethanol extract of manukan leaves (Rhinacanthus nasutus (L) Kurz) against gram-positive bacteria (Staphylococcus aureus) in vitro with the extract concentration of 15, 30, and 60% as a control. This study was a true experiment through the in vitro test with the Kirby Bauer method. The results showed that the average diameter of the inhibition zone was at an extract concentration of 15, 30, and 60% are 9.97 mm, 10.95mm, and 13.18 mm. The Kruskal-Wallis test results showed a significant difference between the four treatment groups (p= 0.010). The Mann-Whitney test showed significant differences in the inhibition zone between control and treatment at 15, 30 and 60% (p= 0.037). In conclusion leave ethanol extract of manukan has antimicrobial activitiy in inhibiting staphylococcus aureus.

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Effectiveness of Ethanolic Extract of Aloe Vera Leaves against Staphylococcus aureus

Medicra (Journal of Medical Laboratory Science/Technology) ◽

10.21070/medicra.v3i2.760 ◽

2020 ◽

Vol 3 (2) ◽

pp. 36-40

Author(s):

Viki Ayu Intan Permatasari ◽

Mutia Hariani Nurjanah ◽

Wimbuh Tri Widodo

Keyword(s):

Staphylococcus Aureus ◽

Aloe Vera ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Average Diameter ◽

Positive Control ◽

Negative Control ◽

Traditional Medicines ◽

Alternative Ingredients ◽

High Concentrations

Since long ago Indonesia used nutritious plants as traditional medicines. Various types of plants in Indonesia can be used as alternative ingredients, one of which is aloe vera. Aloe vera contains saponin and anthraquinone, so aloe vera leaves function as antiseptic and antibacteria. Staphylococcus aureus is a gram-positive coccus bacteria. This bacterium is often found as a normal germ flora in humans. Staphylococcus aureus can cause infections in humans and animals. This study aims to determine the effect of ethanolic extract of Aloe vera leaves in inhibiting Staphylococcus aureus by using maceration extract method. The concentrations used were 20%, 40%, 60%, 80% and 100% with positive control (Erytromycin) and negative control (aquades). The inhibitory zone analysis is done using the table method. Test of ethanol extract of Aloe vera leaves in inhibiting Staphylococcus aureus produced inhibition zones at concentrations of 60%, 80% and 100% with average diameter of 6.94 mm, 6.22 mm and 9.5 mm. The conclusion of this research is the ethanolic extract of Aloe vera leaves can inhibit Staphylococcus aureus in high concentrations

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Uji Aktivitas Ekstrak Etanolik Daun Kemangi (Ocimum sanctum L.) terhadap Staphylococcus aureus secara In Vitr

Jurnal Pharmascience ◽

10.20527/jps.v7i1.8080 ◽

2020 ◽

Vol 7 (1) ◽

pp. 107

Author(s):

Novia Ariani ◽

Dwi Rizki Febrianti ◽

Rakhmadhan Niah

Keyword(s):

Staphylococcus Aureus ◽

Sampling Technique ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Negative Control ◽

Diffusion Method ◽

Ocimum Sanctum ◽

Purposive Sampling ◽

Extract Concentration

ABSTRAK Tanaman kemangi banyak dimanfaatkan oleh masyarakat untuk pengobatan infeksi khususnya bagian daun. Hal ini dikarenakan daun kemangi memiliki senyawa aktif seperti minyak atsiri, alkaloid, saponin, flavonoid, triterpenoid, steroid, tannin dan fenol yang dapat menghambat pertumbuhan bakteri. Tujuan penelitian ini dilakukan untuk mengetahui ada tidaknya aktivitas, mengetahui diameter zona hambat dan mengetahui klasifikasi kekuatan aktivitas daya hambat antibakteri ekstrak etanol daun kemangi. Jenis penelitian ini adalah penelitian eksperimental dengan metode difusi lubang sumuran dengan teknik pengambilan sampel adalah purposive sampling. Konsentrasi ekstrak yang digunakan yaitu konsentrasi 100%, 80%, 60%, 40%, 20%, sedangkan untuk kontrol positif digunakan klindamisin 30µg, dan kontrol negatif yang digunakan etanol 96%. Hasil diameter zona hambat yang terbentuk diukur dengan jangka sorong. Hasil penelitian menunjukkan bahwa ekstrak etanol daun kemangi memiliki aktivitas dalam menghambat pertumbuhan bakteri Staphylococcus aureus dengan diameter rata-rata yang didapat dari setiap perlakuan yaitu 100% (10,08 mm), 80% (8,10 mm), 60% (6,49 mm), 40% (4,29 mm), 20% (2,26 mm), dan sebagai klasifikasi kekuatan aktivitas daya hambat antibakteri yaitu pada konsentrasi 100% kuat, 80%-60% sedang dan 40%-20% lemah. Kata Kunci : Daun kemangi, Ekstrak, Difusi, Staphylooccus aureus ABSTRACT Part of the basil plant (Ocimum sanctum L.) that widely used by people for treatment of infections is basil leaves. This is because basil leaves have active compounds such as essential oils, alkaloids, saponins, flavonoids, triterpenoids, steroids, tannins and phenols which can inhibit bacterial growth. This research aimed to find out the presence or absence of activity, to determine the diameter of the inhibitory zone and the classification of antibacterial mention against what the name of bacterial is activity of ethanol extract of basil leaves. The type of this research is experimental research with a well diffusion method with sampling technique is purposive sampling. The concentration of extracts used were concentrations of 100%, 80%, 60%, 40%, 20%, while as positive control is clindamycin 30µg, and the negative control used 96% ethanol. The resulting diameter of the inhibition zone is measured by the calipers. The results showed that the ethanol extract of basil leaves had an activity in inhibiting the growth of Staphylococcus aureus bacteria with an average diameter obtained from each treatment that was 100% (10,08mm); 80% (8,10mm); 60% (6,49mm); 40% (4,29mm); 20% (2,26mm), and as the antibacterial activity classification, that were strong in 100% of extract concentration, medium in 60-80% of extract concentration, and weak in 20-40% of extract concentration. Keywords : Basil leaf, Extract, Diffusion, Staphylooccus aureus

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Uji Aktivitas Antimikroba Ekstrak Jamur Lingzhi (Ganoderma lucidum) Menggunakan Pelarut Etanol 96% Terhadap Staphylococcus aureus

Journal of Pharmacy and Science ◽

10.53342/pharmasci.v2i2.80 ◽

2017 ◽

Vol 2 (2) ◽

pp. 41-44

Author(s):

Prasetyo Handrianto

Keyword(s):

Staphylococcus Aureus ◽

Ganoderma Lucidum ◽

Ethanol Extract ◽

Inhibition Zone ◽

Negative Control ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Traditional Medicines ◽

Paper Disc

ABSTRAKStaphylococcus aureus merupakan bakteri yang relatif sering dijumpai pada manusia. Beberapa infeksi yang disebabkan oleh Staphylococcus aureus antara lain, bisul, infeksi luka dan pneumonia. Salah satu obattradisional yang dapat digunakan untuk mengobati infeksi yang disebabkan oleh Staphylococcus aureus adalah jamur lingzhi (Ganoderma lucidum). Penelitian dilakukan dengan 5 konsentrasi ekstrak etanol jamur lingzhi, yaitu kontrol negatif (0 µg/ml), 20 µg/ml, 40 µg/ml, 60 µg/ml, 80 µg/ml dan 100 µg/ml dan dilakukan 6 kali pengulangan. Metode yang digunakan dalam uji aktivitas antimikroba yaitu metode difusi kertas cakram. Data zona hambat dianalisis dengan uji Anova One-Way. Hasil penelitian menunjukkan bahwa ekstrak etanol jamurlingzhi (Ganoderma lucidum) dengan konsentrasi 100 µg/ml memiliki rata-rata daya hambat tertinggi yaitu sebesar 18,0 mm (sangat aktif). Dapat disimpulkan bahwa semakin besar konsentrasi ekstrak jamur lingzhi (Ganoderma lucidum), maka akan berpengaruh besar pula terhadap zona hambat bakteri Staphylococcus aureus.Keyword : Antibakteri, Ganoderma lucidum, Staphylococcus aureus, Etanol.ABSTRACTStaphylococcus aureus is a bacteria that can cause infections , such as boils , wound infections and pneumonia. One of the traditional medicines that can be used to treat these infections are fungal lingzhi (Ganodermalucidum). The study was conducted with five concentrations of negative control (0 µg/ml), 20 µg/ml, 40 µg/ml, 60 µg/ml, 80 µg/ml, and 100 µg/ml with six repeats. The method used in the test of antimicrobial activity is paper disc diffusion method. Data of inhibition zone were analyzed by Anova-One Way test. The results showed that the ethanol extract of lingzhi mushroom (Ganoderma lucidum) at concentration 100 µg/ml has highest average of inhibitory regions as big as 18,0 mm. It can be concluded that the greater the concentration of extract of lingzhi mushroom (Ganoderma lucidum) , it will affect the greater the inhibition zone against Staphylococcus aureus.Keyword : Antibakteri, Ganoderma lucidum, Staphylococcus aureus, Etanol.

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PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI REBUSAN DAUN SALAM (Syzygium polyanthum) SECARA IN VITRO

Meditory : The Journal of Medical Laboratory ◽

10.33992/m.v5i2.138 ◽

2018 ◽

Vol 5 (2) ◽

Author(s):

Nyoman Mastra

Keyword(s):

Staphylococcus Aureus ◽

Growth Inhibition ◽

Average Diameter ◽

Inhibition Zone ◽

Negative Control ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Growth Inhibition Zone ◽

Significant Difference

AbstractBackground Staphylococcus aureus is one of causes infection and this bacteria have been resistence for many antibiotic. Bay leaf have antibacterials substance, which stew leaves can be treat infection caused Staphylococcus aureus. The purpose of this study was to determine differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf.Method The method of this study is true experiment with posstest only control design, and used Kirby Bauer disk diffusion method with various concentrations of water stewed of bay leaf (20%, 40%, 60%, 80%,100%), positive control (chloramfenicol 30 μg) and negative control (sterile distilled water). Result The result showed that the average diameter of inhibition zone in concentration 20%, 40%, 60%, 80%, and 100% is 7 mm, 8,4 mm, 9,6 mm, 10,5 mm and 11,5 mm. Based on statistical analysis using oneway ANOVA available the value of p (0,000) α (0,05), so the inhibition zone is significant difference of growth inhibition zone of Staphylococcus aureus at various concentrations of stewed water bay leaf. Conclusion Water stew of bay leaf can inhibit the growth of Staphylococcus aureus, and there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf. Keywords: stew of bay leaf; Staphylococcus aureus; inhibition zone

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PENGARUH VARIASI GELLING AGENT KARBOMER 934 EKSTRAK ETANOL DAUN JERUK PURUT (Citrus hystrix DC) TERHADAP SIFAT FISIK GEL DAN AKTIVITAS ANTIBAKTERI Staphylococcus aureus

Pharma Xplore : Jurnal Ilmiah Farmasi ◽

10.36805/farmasi.v4i1.615 ◽

2019 ◽

Vol 4 (1) ◽

pp. 248-259

Author(s):

Anggun Hari Kusumawati ◽

Siti Hufi Hutami

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Physical Properties ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Inhibition Zone ◽

Gelling Agent ◽

Topical Product ◽

Gel Preparation ◽

Made In

ABSTRAK Ekstrak etanolik daun jeruk purut (Citrus hystrix D.C) mempunyai banyak zat khasiat dimana salah satu zat aktif tersebut berkhasiat sebagai antibakteri. Penggunaan ekstrak etanolik dalam bentuk kental sangat tidak efisien dalam pemakaiannya, sehingga perlu dibuat dalam bentuk sediaan topical, misalnya gel. Penggunaan Karbomer 934 sebagai gelling agent dapat meningkatkan konsistensi basis yang akan berpengaruh terhadap pelepasan zat aktif di dalam gel. Penelitian ini bertujuan untuk mengetahui pengaruh variasi kadar basis Karbomer 934 gel ekstrak etanolik daun jeruk purut terhadap sifat fisik gel dan aktivitas antibakteri terhadap Staphylococcus aureus. Gel dibuat dalam 4 formula dengan konsentrasi basis Karbomer 934 yaitu 0,5 %, 1%, 1,5%, dan 2%. Gel diuji sifat fisik (organolepstis, homogenitas, pH, viskositas, daya lekat, daya sebar, dan uji hedonik) dan daya antibakteri terhadap Staphylococcus aureus. Data dari uji sifat fisik dan pengukuran diameter zona hambat anti bakteri dianalisis dengan statistika Uji Kruskall-Wallis dan Mann-Withney Hasil penelitian dapat disimpulkan bahwa variasi konsentrasi Karbomer 0,5 %, 1 %, 1,5 % dan 2% sebagai gelling agent gel ekstrak etanol daun jeruk purut berpengaruh terhadap sifat fisik gel dan aktifitas antibakteri terhadap Staphylococcus aureus. Semakin besar konsentrasi Karbomer 934, viskositas semakin besar dengan FI= 3079 cP.s, FII= 8135 cP.s, FIII= 13136 cP.s, FIV= 14307 cP.s, menurunkan daya sebar dengan FI= 5.14 cm, FII= 4.04 cm, FIII= 3.51 cm, FIV= 3.35 cm, meningkatakan daya lekat dengan FI= 0.79 cm, FII= 0.87 cm, FIII= 1.05 cm, FIV= 1.16 cm dan menurunkan efektivitas antibakteri dengan FI= 6.4 cm, FII= 5.1, FIII= 5.8 cm dan FIV= 4.9 cm. Kata Kunci : gel, gelling agent, Karbomer 934, Citrus hystrix D.C , Staphylococcus aureus. ABSTRCT Ethanolic extract of kaffir lime leaves (Citrus hystrix D.C) Has many active compounds, antimicrobial activity have been reported numerously. Ethanolic extract of Kaffir Lime leaves can’t directly exposure to the human skin. It can be increased of skin rash and eritema. The extract can be formulated in topical product like gel preparation. The aim of this work was to determine the effect of variations in base levels of Karbomer 934 gel ethanolic extract of kaffir lime leaves on the physical properties of gel and antibacterial activity against Staphylococcus aureus. The gel is made in 4 formulas with a base concentration of Carbomer 934 which is 0.5%, 1%, 1.5%, and 2%. Gel was tested for physical properties (organolepstis, homogeneity, pH, viscosity, adhesion, dispersion, and hedonic test) and antibacterial power to Staphylococcus aureus. Measuring the diameter of the antibacterial inhibition zone after incubation at 37ºC for 24 hours. Data from the physical properties test and measuring the diameter of the anti-bacterial inhibitory zone were analyzed by statistical with Kruskal-Wallis test and Mann-Withney test. The results of this study concluded that variations in Carbomer 934 concentration as gelling agent gel ethanol extract of kaffir lime leaves had an effect on the physical properties of the gel and antibacterial activity against Staphylococcus aureus. The greater concentration of Carbomer 934, increase the viscosity value with FI= 3079 cP.s, FII= 8135 cP.s, FIII= 13136 cP.s, FIV= 14307 cP.s, decrease the spreadability with FI= 5.14 cm, FII= 4.04 cm, FIII= 3.51 cm, FIV= 3.35 cm, increase in adhesion with FI= 0.79 cm, FII= 0.87 cm, FIII= 1.05 cm, FIV= 1.16 cm and decrease the effectiveness of the antibacterial with FI= 6.4 cm, FII= 5.1, FIII= 5.8 cm dan FIV= 4.9 cm. Keywords: gel, gelling agent, Carbomer 934, Citrus hystrix D.C , Staphylococcus aureus.

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Potensi Daun Trembilungan (Begonia hirtella Link) sebagai Antibakteri dan Antifungi

Biosfera ◽

10.20884/1.mib.2016.33.3.309 ◽

2017 ◽

Vol 33 (3) ◽

pp. 126

Author(s):

Febri Nur Ngazizah ◽

Nuraeni Ekowati ◽

Aisyah Tri Septiana

Keyword(s):

Ethyl Acetate ◽

Leaf Extract ◽

Ethyl Acetate Extract ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Inhibitory Zone ◽

Ethyl Palmitate ◽

E Coli ◽

Significant Difference

Begonia hirtella leaf are commonly used by people in mountainous areas to treat itchy due to insect bites and skin infection. This study aims to (1) determine proper solvent extract of B. hirtella leaf on antibacterial activity of S. aureus and E. coli and on antifungal activity of C. albicans (2) determine the lowest concentration of leaf extract of B. hirtella that indicate the formation of inhibition zone (3) know the content of bioactive compounds contained in each leaf extract of B. hirtella. The method used in this study is experimental with completely randomized design (CRD). The treatments were type of solvents (n-hexane, ethyl acetate, ethanol and water) and a serie of the ethyl acetate extract concentration of 500 ppm, 450 ppm, 400 ppm, 350 ppm, 300 ppm and 250 ppm against S. aureus, E. coli and C. albicans. Data were analyzed using analysis of variance (Anova) and the significant differences between the treatments were analyzed by Duncan test at 95% confidence level. The result showed that each solvent extracts affect microbes growth with a highly significant difference p <0.05. Solvent extracts that have the best inhibitory zone is ethyl acetate against S. aureus with an average diameter of inhibitory zone 13.75 ± 1.26 mm. Increasing concentrations of ethyl acetate extract of 250 ppm to 500 ppm increase inhibition zone against microbes. The lowest concentration that show inhibition zone was 300 ppm. The formation inhibition of zone on microbes growth happened due to their compounds in the extract. Extract of n-hexane contains stigmasterol, ethyl acetate contains neophytadiene, while the ethanol extract contains ethyl palmitate

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Uji Aktivitas Antibakteri Ekstrak Etanol Daun Ketapang (Terminalia catappa L.) Warna Hijau dan Warna Merah serta Kombinasinya

Indonesian Journal of Pharmacy and Natural Product ◽

10.35473/ijpnp.v1i2.92 ◽

2018 ◽

Vol 1 (2) ◽

Author(s):

Munira Munira Munira ◽

Rasidah Rasidah Rasidah ◽

Eva Melani Melani ◽

Noni Zakiah Zakiah ◽

Muhammad Nasir Nasir

Keyword(s):

Staphylococcus Aureus ◽

Leaf Extract ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Negative Control ◽

Green Leaf ◽

Diffusion Method ◽

Bacteria Growth ◽

Terminalia Catappa

ABSTRAKDaun ketapang (Terminalia catappa L.) adalah tanaman yang sering dimanfaatkan oleh masyarakat sebagai tanaman obat tradisional. Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak etanol daun ketapang warna hijau, warna merah, kombinasi daun warna hijau dan daun warna merah terhadap pertumbuhan bakteri Staphylococcus aureus. Penelitian ini bersifat eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan yaitu P0 (aquadest sebagai kontrol negatif), P1 (ekstrak etanol daun hijau), P2 (ekstrak etanol daun merah), dan P3 (kombinasi ekstrak etanol daun ketapang berwarna hijau dan merah) dan masing-masing 3 kali ulangan. Uji mikrobiologi menggunakan metode difusi. Berdasarkan hasil uji Anova menunjukkan bahwa ekstrak daun ketapang sangat berpengaruh terhadap pertumbuhan Staphylococcus aureus (P=0,000).Berdasarkan hasil uji lanjut Duncan rata-rata diameter zona hambat yang terbentuk menunjukkan bahwa kombinasi ekstrak daun ketapang warna hijau dan warna merah memiliki zona hambat yang lebih besar (30,92 mm) dan berbeda nyata dengan ekstrak daun ketapang berwarna hijau (21,92 mm), akan tetapi tidak berbeda nyata dengan ekstrak daun ketapang warna merah (26,42 mm).Dari hasil penelitian ini dapat disimpulkan bahwa ekstrak daun ketapang dapat menghambat pertumbuhan bakteri Staphylococcus aureus.Kata kunci: Terminalia catappa L., diameter zona hambat, Staphylococcus aureus Ketapang leaf (Terminalia catappa L.) is a plant that is often used by the community as a traditional medicinal plant. This research was conducted to determine the effect of ethanol extract of green ketapang leaves, red color, combination of green leaf and red leaf color to Staphylococcus aureus bacteria growth. This research was experimental by using Completely Randomized Design (RAL) with 4 treatments which is P0 (aquadest as negative control), P1 (green leaf ethanol extract), P2 (red leaf ethanol extract), and P3 (combination of green leaf ketapang ethanol extract and red) and each of them repeated 3 times. Microbiology test using diffusion method. Based on the results of Anova test showed that ketapang leaf extract greatly affect the growth of Staphylococcus aureus (P=0.000). Based on the results of further test Duncan average diameter of the inhibition zone that was formed showed that the combination of green leaf and red colored leaf extract has a larger inhibition zone (30.92 mm) and significantly different with green leaf ketapang leaf extract (21.92 mm), but not significantly different with the extract of red leaf ketapang (26.42 mm). From the results of this study can be concluded that ketapang leaf extract can inhibit the growth of Staphylococcus aureus.Keywords: Terminalia catappa L., inhibition zone diameter, Staphylococcus aureus

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Potensi Antibakteri Ekstrak Bunga Soka (Ixora coccinea L) terhadap Staphylococcus aureus dan Escherichia coli

AcTion Aceh Nutrition Journal ◽

10.30867/action.v1i2.23 ◽

2016 ◽

Vol 1 (2) ◽

pp. 130

Author(s):

Munira Munira ◽

Riska Maisarah ◽

Muhammad Nasir

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Diffusion Method ◽

Test Results ◽

E Coli ◽

Ethanol Extracts ◽

Ixora Coccinea

Soka plants (Ixora coccineal L.) often used to treat diarrhea, dysentery and wounds. This study was conducted to determine the effect of soka flower ethanol extract in inhibiting the growth of Staphylococcus aureus and Escherichia coli. This study is experimentally using completely randomized design (CRD) with 4 treatments and 6 repetitions which is distilled water as a control, the ethanol extract of soka flowers concentration are 100%, 75%, and 50%. Microbiological test using the diffusion method of Kirby-Baueur. Phytochemical test results soka ethanol extracts of flowers contain alkaloids, flavonoids, tannins, saponins, and triterpenoid. ANOVA test results ethanol extracts of soka flowers greatly affect the growth of S. aureus and E. coli (P=0.000). Duncan test results an average diameter of inhibition zone for the ethanol extract of soka flowers against S. aureus at a concentration of 100% (14.50 mm) was significantly different from the concentration of 75% (10.33 mm) and concentration of 50% (10.67 mm), while the average diameter of inhibition zone for the ethanol extract of soka flowers against E. coli at 100% (11.00 mm) was not significantly different from the 75% (7.83 mm) and 50% (7.50 mm). The ability of ethanol extract of soka flower in inhibition zone against S. aureus greater than E. coli. Keywords: Ixora coccinea L., inhibition zone, Staphylococcus aureus and Escherichia coli.

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