Konsentrasi Hambatan Minimum ekstrak Portulaca grandiflora  terhadap penyakit Motile Aeromonad Septicaemia

The objective of this study was to determine the minimum concentration of extract in inhibiting motile motile aeromonad septicaemia. The material used was the stems and leaves of the plant P. grandiflora as much as 10 g. The crushed simplicia was then macerated with aquades with a ratio of 1:10, then filtered with filter paper and, put in an Erlenmeyer, and evaporated in an incubator at temperature of 30°C. The sensitivity test was performed by placing a paper disc containing extract on the agar medium that had been scratched with bacterial colonies. Clear zone formed around the paper disc was compared with standard classification table for clarifying the inhibition level. The results showed that stem and leaf extract had inhibition zone against Aeromonas hydrophila bacteria that caused motile aeromonad septicaemia. The best result was obtain at concentration of extract 3.25 mg / L) with an average diameter of 22.67 mm and for leaf extract had a moderate to strong zone for all treatments. These proved that stem and leaf extract of P. grandiflora plant had antibacterial activity and can be used as control of MAS disease caused by A. hydrophila bacteria. Keywords: Portulaca grandiflora plant, motile aeromonad septicemia, minimum inbibition concentration

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Potential of Malacca leaf (Phyllanthus emblica) against Salmonella sp

E3S Web of Conferences ◽

10.1051/e3sconf/202015101029 ◽

2020 ◽

Vol 151 ◽

pp. 01029

Author(s):

Nuzul Asmilia ◽

Mahdi Abrar ◽

Yudha Fahrimal ◽

Amalia Sutriana ◽

Yobeswi Husna

Keyword(s):

Ethyl Acetate ◽

Leaf Extract ◽

Ethyl Acetate Extract ◽

Ethanol Extract ◽

Average Diameter ◽

Positive Control ◽

Hexane Extract ◽

Clear Zone ◽

Inhibitory Property

Malacca is one of traditional medicine that possesses a potent antimicrobial activity. This study aims to determine the inhibitory activity of Malacca leaf extract on the growth of Salmonella sp in vitro. The bacteria was obtained from Microbiology Laboratory of the Faculty of Veterinary Medicine, Universitas Syiah Kuala. The study was conducted using n-hexane extract, ethyl acetate extract and ethanol of malacca leaves with dilution concentrations of 5%, 25%, and 50%.The inhibitory property of malacca leaf was tested using Kirby-Bauer method. Data were analyzed descriptively. The results of this study indicate that n-hexane extract, ethyl acetate extract and ethanol extract of malacca leaves can inhibit the growth of Salmonella sp. The n-hexane extract of malacca leaves showed a greater inhibition than the ethyl acetate and ethanol extract of malacca leaves. n-hexane extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 1.35 mm (weak), 4.97 mm (moderate), and 12.87 mm (strong), respectively ethyl acetate extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 2.00 mm (weak), 5.72 mm (moderate), and 7.58 mm (moderate), whereas in ethanol extract were 0.47 mm (weak), 2.58 mm (weak), and 4.35 mm (weak), repectively. The clear zone areas in negative and positive control were 0.00 mm 20.00 mm, respectively. Malacca leaf extract possess inhibitory property against the growth of the Salmonella sp.

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Potensi Daun Teh (Camellia sinensis) dan Daun Anting-anting Acalypha indica L. dalam Menghambat Pertumbuhan Salmonella typhi

JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI ◽

10.36722/sst.v2i2.131 ◽

2013 ◽

Vol 2 (2) ◽

pp. 104

Author(s):

Nita Noriko

Keyword(s):

Camellia Sinensis ◽

Antibacterial Agent ◽

Leaf Extract ◽

Bacterial Resistance ◽

Inhibition Zone ◽

Salmonella Typhi ◽

The Body ◽

Microbiology Laboratory ◽

Clear Zone ◽

Acalypha Indica

Abstrak - Salmonella typhi adalah bakteri penyebab terjadinya penyakit typhus. Pengobatan menggunakan antibiotika menimbulkan resistensi jika dilakukan tidak tuntas terutama ketika sistem tubuh menurun. Pengobatan dengan menerapkan kearifan lokal merupakan salah satu alternatif dalam upaya penyembuhan. Daun teh (Camellia sinensis) dan daun anting-anting (Acalypha indica L) diduga mengandung tanin, suatu senyawa yang dapat berfungsi sebagai antibiotik. Tujuan dari penelitian ini adalah memperoleh informasi efektifitas ekstrak daun teh dan daun anting-anting sebagai antibakteri alami, khususnya S. typhi Penelitian dilakukan pada bulan Maret hingga Oktober 2012 di Laboratorium Mikrobiologi Universitas Al Azhar Indonesia. Tahapan penelitian terdiri dari pembuatan ekstrak daun teh dan anting-anting, pengujian adanya kandungan tanin, penumbuhan S. typhi dan uji antibakteri. Ekstrak daun diperoleh dengan cara perebusan dan pengeringan. Hasil penelitian menunjukkan bahwa ekstrak daun teh dan anting-anting berpotensi untuk dikembangkan sebagai antibiotik. Hal ini ditunjukkan dengan terbentuknya zona bening ketika dilakukan uji antibakteri. Kedua ekstrak daun tersebut positif mengandung tanin yang diduga berperan dalam penghambatan pertumbuhan S. typhi. Abstract - Salmonella typhi is a bacteria that caused typhus disease. The curation using chemical drugs may cause bacterial resistance if the treatment is not complete, especially when the system of the body decreases. Treatment with traditional medicine is one of alternative way for curing typhoid fever. Tea and Anting-anting leaves are suspected to contain tannin, a compound that can act as an antibiotic. The aim of the research is getting information about effectiveness of tea and anting-anting leaf extract as antibacterial agent specially S. thypi. Research were conducted from Maret until October 2012 in Microbiology Laboratory, Al Azhar Indonesia University. The steps incude collecting tea and anting-anting leaves, extracting tannin, testing for tannin, growing the bacteria, and testing the inhibition zone. Leaf extract obtained by boiling and drying. The research shows that leaf tea and anting-anting extraction have potential to be developed as an antibiotic. That are showed by clear zone as indicate inhibition Salmonella typhi when tested antibacterial. Both of the leaves plant are positive tannin containing, and it is indicated as inhibitor S. thypi growth.

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Aktivitas Antibakteri Ekstrak Jamur Lingzhi (Ganoderma lucidum) Menggunakan Pelarut Etanol terhadap Escherichia coli.

Journal of Pharmacy and Science ◽

10.53342/pharmasci.v2i1.64 ◽

2017 ◽

Vol 2 (1) ◽

pp. 33-35

Author(s):

Prasetyo Handrianto

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Ganoderma Lucidum ◽

Herbal Remedy ◽

Transmembrane Protein ◽

Ethanol Extract ◽

Inhibition Zone ◽

Clear Zone ◽

Porin Protein ◽

Paper Disc

ABSTRAKJamur Lingzhi (Ganoderma lucidum) merupakan herbal yang memiliki aktivitas antibiotik atau antibakteri. Triterpenoid dalam Jamur Lingzhi mengandung senyawa yang menunjukkan aktivitas antibakteri melaluimekanisme pengikatan polimer yang kuat terhadap porin (protein transmembran) pada membran luar dari dinding sel bakteri sehingga menyebabkan kerusakan pada porin. Tujuan penelitian ini adalah menentukan efek ekstrak etanol Jamur Lingzhi dalam menghambat pertumbuhan bakteri Escherichia coli. Metode yang digunakan adalah metode cakram dengan mengamati zona hambat yang muncul disekitar cakram. Kosentrasi ekstrak yang digunakan adalah 20 µg / ml, 40 µg / ml, 60 µg / ml, 80 µg / ml, 100 µg / ml. Kosentrasi ekstrak 20µg / ml dan 40 µg / ml menghasilkan zona hambat masing-masing 10,5 mm dan 12,4 mm yang dikategorikan sebagai kurang aktif, sedangkan kosentrasi 60 µg / ml, 80 µg / ml, dan 100 µg / ml menghasilkan zona hambat 13,65 mm, 14,9 mm, dan 16,5 mm yang dikategorikan sebagai aktif. Hasil menunjukkan ekstrak etanol Jamur Lingzhi dapat menghambat pertumbuhan bakteri Escherichia coli.Kata kunci : Ganoderma lucidum, Escherichia coli, Ekstrak etanol, Antibakteri.ABSTRACTLingzhi mushroom (Ganoderma lucidum) is a herbal remedy that has as an antibiotic or antibacterial activity. Lingzhi mushroom triterpenoids containing compounds which possess antibacterial activity by reacting with porin mechanisms (transmembrane protein) on the outer membrane of the bacterial cell wall, forming a strong bond polymers that cause damage porin. The purpose of this study was to was to determine the effect of Lingzhi mushroom extract (Ganoderma lucidum) using ethanol in inhibiting the growth of bacteria Escherichia coli. The method used was paper disc method by observing the clear zone that is visible around the paper disc. And concentration used was 20 µg / ml, 40 µg / ml, 60 µg / ml, 80 µg / ml, 100 µg / ml. At a concentration of 20 µg /ml and 40 µg / ml produce inhibition zone of 10.5 mm and 12.4 mm by category inhibition zone less active and at a concentration of 60 µg / ml, 80 µg / ml, 100 µg / ml produce inhibition zone 13.65 mm by 14.9 mm and 16.5 mm in the active category. The results obtained show that the extract of lingzhi mushroom (Ganoderma lucidum) using ethanol effect on the growth of the bacterium Escherichia coli.Keywords: Ganoderma lucidum , Escherichia coli, Ethanol extract, Antibacterial.

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UJI BIOAKTIVITAS EKSTRAK DAUN BIDARA BIDARA (ZIZIPHUS MAURITIANA LAM) TERHADAP PERTUMBUHAN STAPHYLOCOCCUS AUREUS

Jurnal Kesehatan Panrita Husada ◽

10.37362/jkph.v4i1.181 ◽

2019 ◽

Vol 4 (1) ◽

pp. 49-57

Author(s):

Aan Yulianingsih ◽

Dzikra Arwie

Keyword(s):

Staphylococcus Aureus ◽

Leaf Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Ziziphus Mauritiana ◽

Diffusion Test ◽

Normal Microflora ◽

Inhibitory Power

The background of this study is Staphylococcus aureus is an anaerobic facultative bacterium and one of the normal microflora in the mouth. However, if it is influenced by predisposing factors, it will become a pathogen. Bidara leaves have antimicrobial activity against Staphylococcus aureus, because Bidara leaves contain various compounds including alkaloids, flavonoids, steroids, tannins and essential oils. The purpose of this study was to determine how much inhibitory power produced by Bidara leaf extract on the growth of Staphylococcus aureus bacteria. This research method is a laboratory experimental research using the diffusion test (wells) method. The sample of this study was Staphylococcus aureus. Dilution of Bidara leaf extract consists of 5 concentrations including: 20%, 40%, 60%, 80%, and 100%. Chlorampheniocol is used for Positive control while Negative control uses aquadest. Based on observations show that the average diameter of inhibitory zone Bidara leaf extract at a concentration of 20% with a diameter of 10.5 mm (R), at a concentration of 60% and 80% with a diameter of 16 mm and 18 mm (I), at a concentration of 100% with a diameter of 24 mm (S). It can be concluded that the higher the concentration of Bidara leaf extract, the inhibition zone formed will increase. So Bidara leaves are good for use in treating infections caused by Staphylococcus aureus bacteria.

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Uji Aktivitas Kombucha Teh dan Kopi Sebagai Antibakteri Bakteri Gram Positif dan Bakteri Gram Negatif

Journal of Pharmacy and Science ◽

10.53342/pharmasci.v4i2.135 ◽

2019 ◽

Vol 4 (2) ◽

pp. 61-65

Author(s):

Surahmaida . ◽

Kinanti Ayu Puji Lestari

Keyword(s):

Black Tea ◽

Inhibition Zone ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Clear Zone ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Different Types ◽

Paper Disc

ABSTRAKPenelitian ini bertujuan untuk mengetahui kemampuan Kombucha dalam menghambat pertumbuhan bakteri Gram positif dan Gram negatif. Metode yang digunakan dalam penelitian ini meliputi pembuatan Kombucha dengan variasi bahan dasar (teh hitam, teh hijau dan kopi) dengan jenis gula yang berbeda (gula pasir dan gula stevia); dan uji antibakteri menggunakan metode kertas cakram (difusi agar) terhadap bakteri Gram positif dan Gram negatif. Hasil penelitian menunjukkan ke-6 varian Kombucha tidak berpengaruh atau tidak adanya zona bening (zona hambat) yang terbentuk di sekitar kertas cakram uji pada semua bakteri uji.Kata kunci: Kombucha teh dan kopi, bakteri Gram positif dan bakteri Gram negatif, zona hambat ABSTRACTThe aim of this tudy is to determine the ability of Kombucha to inhibit the growth of Gram positive and Gram negative bacteria. The method used in this study included the making of Kombucha with a variety of basic ingredients (black tea, green tea and coffee) with different types of sugar (sugar and stevia sugar); and antibacterial tests using the paper disc (agar diffusion) method against Gram positive and Gram negative bacteria. The results showed that the 6 variants of Kombucha had no effect or absence of a clear zone (inhibition zone) formed around the test disc paper in all test bacteria.Keywords: Tea and coffee Kombucha, Gram positive bacteria and Gram negative bacteria, inhibitory zone

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Potensi Daun Trembilungan (Begonia hirtella Link) sebagai Antibakteri dan Antifungi

Biosfera ◽

10.20884/1.mib.2016.33.3.309 ◽

2017 ◽

Vol 33 (3) ◽

pp. 126

Author(s):

Febri Nur Ngazizah ◽

Nuraeni Ekowati ◽

Aisyah Tri Septiana

Keyword(s):

Ethyl Acetate ◽

Leaf Extract ◽

Ethyl Acetate Extract ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Inhibitory Zone ◽

Ethyl Palmitate ◽

E Coli ◽

Significant Difference

Begonia hirtella leaf are commonly used by people in mountainous areas to treat itchy due to insect bites and skin infection. This study aims to (1) determine proper solvent extract of B. hirtella leaf on antibacterial activity of S. aureus and E. coli and on antifungal activity of C. albicans (2) determine the lowest concentration of leaf extract of B. hirtella that indicate the formation of inhibition zone (3) know the content of bioactive compounds contained in each leaf extract of B. hirtella. The method used in this study is experimental with completely randomized design (CRD). The treatments were type of solvents (n-hexane, ethyl acetate, ethanol and water) and a serie of the ethyl acetate extract concentration of 500 ppm, 450 ppm, 400 ppm, 350 ppm, 300 ppm and 250 ppm against S. aureus, E. coli and C. albicans. Data were analyzed using analysis of variance (Anova) and the significant differences between the treatments were analyzed by Duncan test at 95% confidence level. The result showed that each solvent extracts affect microbes growth with a highly significant difference p <0.05. Solvent extracts that have the best inhibitory zone is ethyl acetate against S. aureus with an average diameter of inhibitory zone 13.75 ± 1.26 mm. Increasing concentrations of ethyl acetate extract of 250 ppm to 500 ppm increase inhibition zone against microbes. The lowest concentration that show inhibition zone was 300 ppm. The formation inhibition of zone on microbes growth happened due to their compounds in the extract. Extract of n-hexane contains stigmasterol, ethyl acetate contains neophytadiene, while the ethanol extract contains ethyl palmitate

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PENGUJIAN EFEKTIFITAS FORMULA GEL EKSTRAK DAUN BELUNTAS (Pluchea indica (L.) Less) DENGAN VARIASI KONSENTRASI GELLING AGENT SEBAGAI KANDIDAT SEDIAAN ANTI JERAWAT

Jurnal Ilmiah Manuntung ◽

10.51352/jim.v6i2.393 ◽

2020 ◽

Vol 6 (2) ◽

pp. 312

Author(s):

Andi Ulfah Magefirah Rasyid ◽

Zahira Amody

Keyword(s):

Experimental Research ◽

Leaf Extract ◽

Propionibacterium Acnes ◽

Physical Stability ◽

Average Diameter ◽

Inhibition Zone ◽

Stability Test ◽

Gelling Agent ◽

Antimicrobial Compounds ◽

Accelerated Stability

Beluntas (Pluchea indica (L.) Less) is a plant that is often used in traditional medicine because of the presence of several substances that have activity as antimicrobial compounds. Beluntas leaves contain secondary metabolite compounds such as flavonoids, alkaloids, tannins, steroids, terpenoids and saponins which can function as antibacterial. This experimental research aimed at obtaining beluntas leaf extract gel and to determine the physical stability and the effectiveness of beluntas extract gel against Propionibacterium acnes. The first step is drying the beluntas leaves and and extracted by maceration method with 70% ethanol solvent. The extract obtained was then formulated into a gel and evaluated, the evaluation results show that formula 1 is the most stable. The results showed that beluntas leaf extract gel was effective in inhibiting the growth of Propionibacterium acnes, the inhibition zone obtained was categorized as strong, where the average diameter before the accelerated stability test was 12.02 mm and 11.58 mm after the accelerated stability test.

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FORMULASI DAN UJI EFEKTIVITAS ANTIBAKTERI SEDIAAN GEL EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) SEBAGAI ANTISEPTIK TANGAN

PHARMACON ◽

10.35799/pha.8.2019.29335 ◽

2019 ◽

Vol 8 (3) ◽

pp. 580

Author(s):

Thesya Manarisip ◽

Paulina V.Y Yamlean ◽

Widya Astuty Lolo

Keyword(s):

Staphylococcus Aureus ◽

Leaf Extract ◽

Average Diameter ◽

Clear Zone ◽

Leaf Extracts ◽

Cycling Test ◽

Power Testing ◽

Gel Preparation ◽

Colony Counter ◽

Muntingia Calabura

Kerson Fruit (Muntingia calabura L.) contains bioactive compounds, such as flavonoids, saponins, triterpenes, steroids, and tannins, which are potentially as antibacterial compounds. The aim of this study was to make a formulation of hand antiseptic gel from kerson leaf extracts with three variations in extract concentrations of 5%, 10%, and 15%, and to test the effectiveness of antibacterial preparations against Staphylococcus aureus bacteria. The method of this research is laboratory experimental research. Kerson extracts was obtained by maceration using 96% ethanol. The results showed that kerson leaf extract can be formulated as a hand antiseptic gel preparation that meets organoleptic requirements, like homogeneity, pH, dispersion, consistency, adhesion, cycling test, and antiseptic power testing using a colony counter. On the results of antibacterial effectiveness testing, there is a clear zone that presents the ability to inhibit the growth of gel test bacteria. The average diameter of the hand antiseptic gel preparation of kersen leaf extract at concentrations of 5%, 10%, and 15% respectively were 10.00 mm, 11.66 mm and 12.00 mm so that the ability of inhibition of gel test bacteria in all concentrations was categorized strong. Keywords: Kerson, Muntingia calabura L, Hand Antiseptic Gel, Staphylococcus aureus. ABSTRAK Kersen (Muntigia calabura L.) mengandung senyawa bioaktif yaitu senyawa flavonoid, saponin, triterpen, steroid, dan tannin yang merupakan senyawa yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk membuat formulasi sediaan gel antiseptik tangan dari ekstrak daun kersen dengan tiga variasi konsentrasi ekstrak yakni 5%, 10%, dan 15%, serta menguji efektivitas antibakteri sediaan terhadap bakteri Staphylococcus aureus. Metode penelitian ini merupakan penelitian eksperimental laboratorium. Ekstrak tanaman kersen diperoleh dengan cara maserasi menggunakan pelarut etanol 96%. Hasil penelitian menunjukkan bahwa ekstrak daun kersen dapat diformulasikan sebagai sediaan gel antiseptik tangan yang memenuhi persyaratan organoleptik, homogenitas, pH, daya sebar, konsistensi, daya lekat, cycling test, dan pengujian daya antiseptik dengan menggunakan alat colony counter. Pada hasil pengujian efektivitas antibakteri, terdapat zona bening yang mempresentasikan kemampuan penghambatan pertumbuhan bakteri uji oleh gel. Diameter rata-rata sediaan gel antiseptik tangan ekstrak daun kersen pada konsentrasi 5%, 10%, dan 15% berturut-turut yaitu 10,00 mm , 11,66 mm dan 12,00 mm sehingga kemampuan penghambatan bakteri uji oleh gel disemua konsentrasi dikategorikan kuat. Kata kunci: Kersen, Muntingia calabura L, Gel Antiseptik Tangan, Staphylococcus aureus.

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Uji Aktivitas Antibakteri Ekstrak Etanol Daun Ketapang (Terminalia catappa L.) Warna Hijau dan Warna Merah serta Kombinasinya

Indonesian Journal of Pharmacy and Natural Product ◽

10.35473/ijpnp.v1i2.92 ◽

2018 ◽

Vol 1 (2) ◽

Author(s):

Munira Munira Munira ◽

Rasidah Rasidah Rasidah ◽

Eva Melani Melani ◽

Noni Zakiah Zakiah ◽

Muhammad Nasir Nasir

Keyword(s):

Staphylococcus Aureus ◽

Leaf Extract ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Negative Control ◽

Green Leaf ◽

Diffusion Method ◽

Bacteria Growth ◽

Terminalia Catappa

ABSTRAKDaun ketapang (Terminalia catappa L.) adalah tanaman yang sering dimanfaatkan oleh masyarakat sebagai tanaman obat tradisional. Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak etanol daun ketapang warna hijau, warna merah, kombinasi daun warna hijau dan daun warna merah terhadap pertumbuhan bakteri Staphylococcus aureus. Penelitian ini bersifat eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan yaitu P0 (aquadest sebagai kontrol negatif), P1 (ekstrak etanol daun hijau), P2 (ekstrak etanol daun merah), dan P3 (kombinasi ekstrak etanol daun ketapang berwarna hijau dan merah) dan masing-masing 3 kali ulangan. Uji mikrobiologi menggunakan metode difusi. Berdasarkan hasil uji Anova menunjukkan bahwa ekstrak daun ketapang sangat berpengaruh terhadap pertumbuhan Staphylococcus aureus (P=0,000).Berdasarkan hasil uji lanjut Duncan rata-rata diameter zona hambat yang terbentuk menunjukkan bahwa kombinasi ekstrak daun ketapang warna hijau dan warna merah memiliki zona hambat yang lebih besar (30,92 mm) dan berbeda nyata dengan ekstrak daun ketapang berwarna hijau (21,92 mm), akan tetapi tidak berbeda nyata dengan ekstrak daun ketapang warna merah (26,42 mm).Dari hasil penelitian ini dapat disimpulkan bahwa ekstrak daun ketapang dapat menghambat pertumbuhan bakteri Staphylococcus aureus.Kata kunci: Terminalia catappa L., diameter zona hambat, Staphylococcus aureus Ketapang leaf (Terminalia catappa L.) is a plant that is often used by the community as a traditional medicinal plant. This research was conducted to determine the effect of ethanol extract of green ketapang leaves, red color, combination of green leaf and red leaf color to Staphylococcus aureus bacteria growth. This research was experimental by using Completely Randomized Design (RAL) with 4 treatments which is P0 (aquadest as negative control), P1 (green leaf ethanol extract), P2 (red leaf ethanol extract), and P3 (combination of green leaf ketapang ethanol extract and red) and each of them repeated 3 times. Microbiology test using diffusion method. Based on the results of Anova test showed that ketapang leaf extract greatly affect the growth of Staphylococcus aureus (P=0.000). Based on the results of further test Duncan average diameter of the inhibition zone that was formed showed that the combination of green leaf and red colored leaf extract has a larger inhibition zone (30.92 mm) and significantly different with green leaf ketapang leaf extract (21.92 mm), but not significantly different with the extract of red leaf ketapang (26.42 mm). From the results of this study can be concluded that ketapang leaf extract can inhibit the growth of Staphylococcus aureus.Keywords: Terminalia catappa L., inhibition zone diameter, Staphylococcus aureus

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Uji Daya Hambat Sari Daun Alpukat (Persea americana mill) terhadap Pertumubuhan Escherichia coli

Health Information : Jurnal Penelitian ◽

10.36990/hijp.v9i2.70 ◽

2017 ◽

Vol 9 (2) ◽

pp. 58-64

Author(s):

Tuty Yuniarty ◽

Lisfaresliana Hasjim

Keyword(s):

Escherichia Coli ◽

Pathogenic Bacteria ◽

Leaf Extract ◽

Inhibition Zone ◽

Persea Americana ◽

Clear Zone ◽

Traditional Medicines ◽

Experimental Laboratory ◽

Escherichia Coli Bacteria ◽

Static Group

Avocado leaves (Percea americana mill) are a part of avocado plants that have benefits as traditional medicines. Avocado leaves are potentially used as anti-diarrhea based on the content of chemicals contained therein, namely saponins, alkaloids, tannins, flavanoids, polyphenols, quercetin which are used to kill pathogenic bacteria, one of which is Escherichia coli. Escherichia coli is an opportunist germ that is commonly found in the human intestine as a normal flora. The purpose of this study was to determine the inhibition of avocado leaf extract on the growth of Escherichia coli bacteria. This type of research is an Experimental laboratory. The research design used in this study was static group comparison because this study was conducted to see differences in concentrations of 10%, 15%, 25%, 50% and 75% of avocado leaf extract in inhibiting the growth of Escherichia coli bacteria by looking at the clear zone formed. The results showed that at a concentration of 25%, 50% and 75% a clear zone (inhibition zone) was formed, whereas at concentrations of 10% and 15% no clear zone (inhibition zone) was formed. From the results of this study it can be concluded that avocado leaf extract can inhibit the growth of Escherichia coli at concentrations of 25%, 50% and 75%. Catatan PenerbitPolekkes Kemenkes Kendari menyatakan tetap netral sehubungan dengan klaim dari perspektif atau buah pikiran yang diterbitkan dan dari afiliasi institusional manapun.

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