scholarly journals UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KEJI BELING (Strobilanthes crispus L) PADA TIKUS PUTIH GALUR WISTAR (Rattus norvegicus)

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 170
Author(s):  
Ariesthya D. C. Olii ◽  
Widdhi Bodhi ◽  
Hosea J. Edi
Keyword(s):  
Analgesic Effect ◽  
Emotional Experience ◽  
White Male ◽  
Ethanol Extract ◽  
Male Rats ◽  
Test Material ◽  
Analgesic Effects ◽  
Wistar Strain ◽  
Strobilanthes Crispus ◽  
One Way Anova

ABSTRACT Pain is an unpleasant sensory and emotional experience due to tissue damage both actual and potential. Analgesics are a drug used to relieve pain without losing consciousness. Keji beling leaves (Strobilanthes crispus L) contain glycosidic ester compounds from caffeic acid, which have analgesic effects. This study aims to determine whether there is an analgesic effect on the ethanol extract of keji beling leaves with different doses, namely dose 0.0023g / 200gBW, 0.0047g / 200gBW, and 0.0094g / 200gBW in test animals of wistar strain male rats. This study uses a laboratory experimental research design using wistar strain male white rat as a test animal. The analgesic effect test was carried out by the heat stimulation method using a 53 °C water bath. The rat response was observed in the form of movements of licking the hind legs or jumping from before giving test material and after giving test material at 30, 60, 90 and 120 minutes, respectively. From the results of statistical data analysis with One Way Anova and LSD showed that the extracts keji beling leaves has an analgesic effect. Where among those three doses the best dose was 0.0023g / 200gBW. Keywords: Analgesic, anova, keji beling leaves, white male wistar rats. ABSTRAK Rasa nyeri merupakan pengalaman sensorik dan emosional yang tidak menyenangkan akibat kerusakan jaringan baik aktual maupun potensial. Analgesik ialah suatu obat yang digunakan untuk menghilangkan rasa sakit tanpa menghilangkan kesadaran. Daun keji beling (Strobilanthes crispus  L) mengandung senyawa glikosidik ester dari asam caffeic yang memiliki efek analgesik. Penelitian ini bertujuan untuk mengetahui ada tidaknya efek analgesik pada ekstrak etanol daun keji beling dengan dosis berbeda-beda yaitu dosis 0,0023g/200gBB, 0,0047g/200gBB, dan 0,0094g/200gBB pada hewan uji tikus putih jantan galur wistar. Penelitian ini menggunakan Rancangan penelitian eksperimental laboratorium dengan menggunakan hewan uji tikus putih jantan galur wistar. Pengujian efek analgesik dilakukan dengan metode rangsangan panas menggunakan waterbath suhu 53°C. Respon tikus diamati berupa gerakan menjilat kaki belakang atau melompat dari sebelum pemberian bahan uji dan sesudah pemberian bahan uji  berturut-turut pada menit ke 30, 60, 90 dan 120. Dari hasil analisis data secara statistika dengan One Way Anova dan LSD menunjukkan bahwa ekstrak daun keji beling memiliki efek analgesik. Dimana antara ketiga dosis tersebut yang paling terbaik ialah dosis 0,0023 g/200gBB. Kata kunci : Analgesik, Anova, Daun Keji Beling, tikus putih jantan galur wistar

scholarly journals UJI EFEKTIVITAS SENYAWA FLAVONOID DARI EKSTRAK ETANOL DAUN SAWILANGIT (Vernonia cinerea (L.) Less) SEBAGAI ANTIPIRETIK PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus) YANG DIINDUKSI VAKSIN DPT

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 572
Author(s):  
Asriyani Samiun ◽  
Edwin De Queljoe ◽  
Irma Antasionasti
Keyword(s):  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Male Rats ◽  
Test Material ◽  
Control Group ◽  
White Rats ◽  
Wistar Strain ◽  
One Way Anova ◽  
Vernonia Cinerea ◽  
Dpt Vaccine

ABSTRACTSawilangit leaves (Vernonia Cinerea (L.) Less) is a plant that contains flavonoids as an antipyretic. The purpose of this study was to determine the effectiveness of flavonoid compounds from ethanol extract of sawilangit (Vernonia cinerea (L.) Less) leaves as an antipyretic in male white rats Wistar strain (Rattus norvegicus) induced by DPT vaccine. A total of 15 male rats were divided into 5 treatment groups, namely the negative control group (CMC 1%), the positive control group (paracetamol), and the ethanol extract group of sawilangit leaves with a dose of 200 mg, a dose of 400 mg, a dose of 800 mg. Each mouse was induced intramuscularly with a DPT vaccine at a dose of 0.3 ml. Temperature measurements were carried out before and after induction, then every 30 minutes after giving the test material from the 30th minute to the 180th minute. Data were analyzed statistically with One Way ANOVA and continued with LSD test to see significant differences between treatments. The results showed that the ethanol extract of sawilangit leaves had an antipyretic effect on male white rats Wistar strain..Key words: Antipyretics, Vernonia Cinerea, male white rats ABSTRAKDaun Sawilangit  (Vernonia Cinerea (L.) Less) adalah salah satu tanaman yang mengandung zat Flavonoid sebagai antipiratik. Tujuan penelitian ini untuk mengetahui adanya efektivitas senyawa flavonoid dari ekstrak etanol daun sawilangit (Vernonia cinerea (L.) Less) sebagai antipiretik pada tikus putih jantan galur wistar (Rattus norvegicus) yang diinduksi vaksin DPT. Sebanyak 15 ekor tikus putih jantan dibagi 5 kelompok perlakuan yaitu kelompok kontrol negatif (CMC 1%), kelompok kontrol positif (parasetamol), dan kelompok ekstrak etanol daun sawilangit dosis 3,6 mg, dosis 7,2 mg, dosis 14,4 mg. setiap tikus diinduksi vaksin DPT dosis 0,3 ml secara intramuscular. Pengukuran suhu dilakuan sebelum dan sesudah induksi, kemudian setiap 30 menit  setelah pemberian bahan uji dari menit ke-30 sampai menit ke-180. Data dianalisis secara statistika dengan One Way ANOVA dan dilanjutkan uji LSD untuk melihat beda nyata antar perlakuan. Hasil penelitian menunjukan bahwa Ekstrak etanol daun sawilangit mempunyai efek antipiretik terhadap tikus putih jantan galur wistar. Kata kunci: Antipiretik, Vernonia Cinerea, tikus putih jantan

scholarly journals UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KELOR (Moringa oleifera Lam.) PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 325
Author(s):  
Alyah A.P Tamimi ◽  
Edwin De Queljoe ◽  
Jainer P. Siampa
Keyword(s):  
Rattus Norvegicus ◽  
Analgesic Effect ◽  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Negative Control ◽  
Test Material ◽  
Control Group ◽  
White Rats ◽  
Wistar Strain ◽  
Moringa Oleifera Lam

ABSTRACTAnalgesic medicinals are medicinals that can inhibit pain. Moringa leaves (Moringa oleifera Lam.) are known to contains alkaloid and flavonoid which have analgesic effects.The purpose of this study was to determine the analgesic effects of the ethanol extract of Moringa leaves on male white rats wistar strain (Rattus norvegicus). A total of 15 rats were divided into 5 treatment groups, namely the negative control group (CMC), positive control group (paracetamol), and the group of ethanol extract of Moringa leaves with different doses of 0.1 g, 0.2 g, and 0.4 g. Rat response was observed before giving test material and after giving test material from the 30th, 60th, 90th, and 120th minutes. Data were analyzed using ANOVA then continued with LSD test. The results showed that the ethanol extract of Moringa leaves with dosages of 0.1 g, 0.2 g, and 0.4 g had an analgesic effect on male white rats wistar strain. And among these three doses the best in providing analgesic effect is a dose of 0.4 g. Keywords: Analgesic, Moringa leaf (Moringa oleifera Lam.), Rattus norvegicus. ABSTRAKObat analgesik merupakan obat yang dapat menghambat rasa nyeri. Daun kelor (Moringa oleifera Lam.) diketahui mengandung alkaloid dan flavonoid yang memiliki efek analgesik. Tujuan penelitian ini untuk mengetahui adanya efek analgesik dari ekstrak etanol daun kelor (Moringa oleifera Lam.) pada tikus putih jantan galur wistar (Rattus norvegicus). Sebanyak 15 ekor tikus dibagi 5 kelompok perlakuan, yaitu kelompok kontrol negatif, kelompok kontrol positif, dan kelompok ekstrak etanol daun kelor dengan dosis berbeda 0,1 g, 0,2 g, dan 0,4 g. Respon tikus diamati sebelum pemberian bahan uji dan setelah pemberian bahan uji dari menit ke 30, 60, 90, dan 120. Data dianalisis menggunakan ANOVA kemudian dilanjutkan uji LSD. Hasil penelitian menunjukkan bahwa ekstrak etanol daun kelor  dengan dosis 0,1 g 0,2 g, dan 0,4 g memiliki efek analgesik terhadap tikus putih jantan galur wistar. Dan diantara ketiga pemberian dosis tersebut yang paling baik dalam memberikan efek analgesik yaitu dosis 0,4 g. Kata kunci: Analgesik, Daun Kelor (Moringa oleifera Lam.), Rattus norvegicus.

scholarly journals UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KUCAI (Allium tuberosum) PADA TIKUS PUTIH JANTAN (Rattus norvegicus)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 413
Author(s):  
Dwilanda E. Turama ◽  
Widdhi Bodhi ◽  
Imam Jayanto
Keyword(s):  
Analgesic Effect ◽  
Ethanol Extract ◽  
Test Material ◽  
Hot Plate ◽  
Allium Tuberosum ◽  
White Rats ◽  
Wistar Strain ◽  
Before And After ◽  
Stimulation Method ◽  
Group 2

ABSTRACTKucai (Allium tuberosum) leaves contain metabolite compounds such as alkaloids, saponins, tannis which are potential sources to be developed as medical plants. Flavonoid is a derivative pf a phenol that has an analgesic properties. This study aims to determine whether there is an analgesic effect on the ethanol extract of kucai leaves. This study uses a laboratory experimental research design using wistar strain male white rats as an experiment animal. The ethanol extract of kucai leaves are given with different doses, group 1 doses 3,6 mg, group 2 doses 7,2 mg and group 3 doses 14,4 mg. Stimulation method in rats was carried out by using a 55°C hot plate. Rats was observed before and after giving test material at 30, 60, 90 and 120 minutes respectively and observed their responses by the form of licking legs and jumping. From the result of data in the form of tables and charts show that the ethanol extract of kucai leaves with doses 3,6 mg, 7,2 mg, and 14,4 mg has analgesic effect. It can be concluded that the best analgesic effect of doses is doses 14,4 mg.Keyword : Analgesic, kucai, male white ratsABSTRAK Daun Kucai (Allium tuberosum) memiliki kandungan senyawa metabolit seperti alkaloid, saponin, tanin, dan flavonoid yang merupakan sumber potensial untuk dikembangkan sebagai tanaman obat. Flavonoid merupakan turunan senyawa fenol yang memiliki sifat analgetik. Penelitian ini bertujuan untuk mengetahui adanya efek analgesik dari ekstrak etanol daun kucai. Penelitian ini menggunakan Rancangan Penelitian eksperimental laboratorium menggunakan tikus putih jantan galur wistar sebagai hewan percobaan. Ekstrak etanol daun kucai diberikan dengan dosis yang berbeda-beda yaitu kelompok perlakuan 1 dengan dosis 3,6 mg, perlakuan 2 dengan dosis 7,2 mg, perlakuan 3 dengan dosis 14,4 mg. Metode rangsangan pada tikus dilakukan dengan menggunakan hot plate dengan suhu 550C. Tikus di amati sebelum dan setelah pemberian bahan uji, menit ke 30, 60, 90, dan 120. Respon tikus yang diamati berupa menjilat kaki dan melompat. Dari hasil analisis data dalam bentuk tabel, grafik dan diagram menunjukkan bahwa ekstrak etanol daun kucai dengan dosis 3,6 mg, 7,2 mg, dan 14,4 mg memiliki efek analgesik. Dapat disimpulkan bahwa dosis yang memiliki efek analgesik terbaik ialah dosis 14,4 mg.Kata kunci: Analgesik, kucai, tikus putih jantan

UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) PADA MENCIT PUTIH JANTAN (Mus musculus) DENGAN INDUKSI NYERI ASAM ASETAT

2017 ◽  
Vol 2 (2) ◽  
pp. 147
Author(s):  
Triswanto Sentat ◽  
Susiyanto Pangestu
Keyword(s):  
Analgesic Effect ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Treatment ◽  
Treatment Groups ◽  
Analgesic Effects ◽  
Significant Difference ◽  
Analgesic Dose ◽  
Muntingia Calabura

Kersen leaf (Muntingia calabura L.) contains tannins, flavonoids and polyphenol compounds allegedly have analgesic effect. The objective was to determine the analgesic effect of ethanol extract of kersen leaves and to determine the most effective analgesic dose. This study was an experimental research. Leaves were extracted with ethanol 70% and the analgesic effect test was divided into 5 groups: negative control treatment (distilled water), positive control (mefenamic acid 2.6mg/kg), kersen leaf ethanol extract first dose (100mg/kg), second dose (200mg/kg) and tthird dose (400mg/kg). Giving treatments by oral, after 30 minutes, the mices were given a pain inductor with 0.5% acetic acid by intra peritonial administration. Analgesic power was calculated by counting the number of writhing in mice for 1 hour. The results showed that the ethanol extract of cherry leaf has analgesic effect. From the calculation of the first dose analgesic power (42.9%), second dose (59.4%) and the third dose 69.9%. Statistical test results kruskal wallis value of p=0.011 (p<0.05) showed a significant difference between all analgesic treatment groups. The conclusion of this study is all of the ethanol extract had analgesic effects on male white mice, whereas a dose of 400mg/kg is the most effective analgesic dose.

scholarly journals Effect of food products on endogenous generation of n−nitrosamines in rats

1997 ◽  
Vol 78 (2) ◽  
pp. 335-345 ◽  
Author(s):  
V. K. Atanasova-Goranova ◽  
P. I. Dimova ◽  
G. T. Pevicharova
Keyword(s):  
White Male ◽  
Food Products ◽  
Inhibitory Effect ◽  
Male Rats ◽  
Aronia Melanocarpa ◽  
Tomato Paste ◽  
Wistar Strain ◽  
Effect Of Food ◽  
Black Chokeberry ◽  
Β Carotene

An experiment was conducted to study the efficacy of two tomato pastes and aronia nectar (fruit juice + pulp from the black chokeberry, Aronia melanocarpa Elliot) as inhibitors of nitrosamine production in cancer prophylaxis programmes. White male rats of the Wistar strain were employed in an acute trial. Aminopyrin + sodium nitrite (APSN) were used as precursors for generation of endogenous nitrosamine. The animals were allocated to different dietary groups and fed by intubation with APSN or APSN + food products. Introduction of tomato paste (TP), high-β-carotene tomato paste (HCTP) and aronia nectar (AN) as inhibitors of n−nitrosamine formation exerted a positive effect on blood and liver variables which was demonstrated by decreased concentrations of glutamic-oxaloacetic transaminase (EC 2.6.1.1), glutamic-pyruvic transaminase (EC 2.6.1.2) and uric acid in serum and lipid content in hepatocytes. Animals treated with APSN developed dystrophic changes in liver such as centrolobular necrosis, intense exangia, and enlarged cells with two, often large, pyknotic nuclei, while the structure of livers of rats fed with TP, HCTP or AN was well protected and almost normal. TP had a particularly beneficial effect on serum total protein and albumin concentrations as had AN on the urea value. The inhibitory effect of th food products used is explained by their chemical nature including pH, ascorbic index (ascorbate:nitrate), lycopene and β-carotene contents.

scholarly journals Paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male rats

2015 ◽  
Vol 34 (2) ◽  
pp. 87 ◽  
Author(s):  
Fitranto Arjadi ◽  
Ginus Partadireja ◽  
Lientje Setyawan Maurits ◽  
Mulyono Pangestu
Keyword(s):  
Sleep Deprivation ◽  
Caspase 3 ◽  
Paradoxical Sleep ◽  
White Male ◽  
Male Rats ◽  
Paradoxical Sleep Deprivation ◽  
Treatment Groups ◽  
Footshock Stress ◽  
Post Hoc ◽  
One Way Anova

BACKGROUND<br />Sleep deprivation is a significant problem among adult men and is considered as a risk factor for several diseases. Paradoxical sleep deprivation (PSD) induces Leydig cell apoptosis through elevation of corticosterone, with testicular malondialdehyde (MDA) and Leydig cell caspase-3 expression as parameters. The aim of this study was to observe testicular MDA level and caspase-3 expression treated with paradoxical sleep deprivation (PSD), immobilization, and footshock stress and to determine the stress model with a significant effect in white male rats (Rattus norvegicus) . <br /><br />METHODS<br />This experimental randomized study of posttest only with control group design was conducted on 24 white male Wistar strain rats, randomly allocated into four treatment groups, i.e. control (K1) without any stress treatment, PSD (KII), immobilization (KIII), and footshock stress (KIV). Treatments were given for 25 days to produce chronic stress. Testicular MDA concentration was examined by the ELISA method while caspase-3 was examined by the TUNEL method.<br /><br />RESULTS<br />Mean testicular MDA concentration with one-way ANOVA test showed differences in means between the groups (p=0.000) and post hoc Tukey-HSD test showed significant results between PSD stress group versus control, immobilization and footshock stress groups. One-way ANOVA test showed a significant difference in caspase-3 expression in at least two treatment groups (p=0.008) and post-hoc Tuckey-LSD test showed significant differences between controls and all stress groups. <br /><br />CONCLUSION<br />Sleep deprivation is a type of stress inducing changes in testicular MDA concentration and caspase-3 expression in male rat testes.

scholarly journals UJI EFEK ANALGETIK EKSTRAK ETANOL RIMPANG JAHE PUTIH (Zingiber officinale Rosc.var. Amarum) PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus)

PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 927
Author(s):  
Beatriks Lahamendu ◽  
Widdhi Bodhi ◽  
Jainer P. Siampa
Keyword(s):  
Treatment Group ◽  
White Male ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Analgesic Effects ◽  
Wistar Strain ◽  
Ginger Rhizome ◽  
Ethanol Extracts

ABSTRACTWhite Ginger Rhizome (Zingiber officinale Rosc.var. Amarum) contains secondary metabolites such as flavonoids, there are also several compounds such as gingerol, shogaol, and zhigeron which provide anti-inflamatory, antioxidant, analgesic, anticarsinogenic and cardiotonic effects. This study aims to determine the analgesic effect of ethanol extracts of white ginger rhizome with different doses, namely the treatment group 1, dose 0.006 g, the Treatment  Group 2, dose 0.012 g and the Treatment Group 3, dose 0.024 g tested on white male wistar strain rats. This study uses a laboratory experimental research design using male white rats as experimental animals. The result of the study using LSD showed a difference between the group of negative control and the group of positive control, and also the treatment group with extracts with significance value (0.014, 0.008, 0.012 and 0.005 ) the value <0.05, which indicates that according to the hypothesis namely rejecting H0 and accepting H1, which means significantly different between the negative control group with  positive control group and the treatment group with extracts, but there is no difference between the positive control group and the treatment group with extract which means there is no difference because they both have analgesic effects. From the results of the study it can be concluded that the ethanol extracts of white ginger rhizome at a dose 0.006 g, 0.012 g, and 0.024 g has analgesic effects in male white wistar strain rats can be seen from the decreasing number of rat responses (licks and jumps).Keywords: Analgesic, anova, White Ginger Rhizome, White male wistar strain ABSTRAKRimpang Jahe putih (Zingiber officinale Rosc.var amarum ) mengandung metabolit sekunder seperti flavonoid juga terdapat beberapa senyawa seperti gingerol, shogaol, dan zingeron yang memberikan efek antiinflamasi, antioksidan, analgetik, antikarsinogenik dan kardiotonik. Penelitian ini bertujuan untuk mengetahui adanya efek analgetik dari ekstrak etanol rimpang jahe putih dengan dosis yang berbeda-beda yaitu Kelompok Perlakuan 1 dosis 0,006 g, kelompok Perlakuan 2 dosis 0,012 g dan Kelompok Perlakuan 3 dosis 0,024 g yang diuji pada tikus putih jantan galur wistar. Penelitian ini menggunakan rancangan penelitian eksperimental laboratorium dengan menggunakan tikus putih jantan sebagai hewan percobaan. Hasil penelitian menggunakan LSD menunjukkan adanya perbedaan antara kelompok kontrol negatif dengan kelompok kontrol positif, dan juga kelompok perlakuan dengan ekstrak dengan nilai signifikansi (0,014, 0,008, 0,012 dan 0,005) nilai tersebut <0,05 yang menandakan bahwa sesuai hipotesis yaitu menolak H0 dan menerima H1 yang berarti berbeda secara signifikan antara kelompok kontrol negatif dengan kontrol positif dan kelompok perlakuan dengan ekstrak,tapi tidak terdapat perbedaan antara kelompok kontrol positif dengan kelompok perlakuan dengan ekstrak yang berarti tidak memiliki perbedaan karena sama-sama memiliki efek analgetik. Dari hasil penelitian dapat disimpulkan bahwa ekstrak etanol rimpang jahe putih dengan dosis 0,006 g, 0,012 g dan 0,024 g memiliki efek analgetik pada tikus putih jantan galur wistar dapat dilihat dari menurunnya jumlah respon tikus (jilatan dan lompatan).Kata kunci : Analgetik, anova, rimpang Jahe putih, tikus Putih jantan galur wistar

scholarly journals Effect of various inductions of sleep deprivation stress on proinflammatory cytokine levels in gingival crevicular fluids of white male Wistar strain rats (Rattus novergicus)

2020 ◽  
Vol 53 (1) ◽  
pp. 24
Author(s):  
Pratiwi Nur Widyaningsih ◽  
Fitranto Arjadi ◽  
Erlina Sih Mahanani
Keyword(s):  
Sleep Deprivation ◽  
Proinflammatory Cytokines ◽  
Paradoxical Sleep ◽  
White Male ◽  
Control Group ◽  
Total Sleep Deprivation ◽  
Wistar Strain ◽  
Tnf Α ◽  
Cytokine Levels ◽  
One Way Anova

Background: Stress that is induced by sleep deprivation can modulate the damage of periodontal tissue by elevating the levels of proinflammatory cytokines (i.e. IL-1β and TNF-α). The effects of sleep deprivation can be resolved with sleep recovery. Gingival crevicular fluid (GCF) is fluid in sulcular gingiva which acts as an oral biomarker for evaluating periodontal abnormalities. Purpose: The aim of this study was to determine the effect of various induction methods of sleep deprivation stress on cytokine levels in GCF of white male Wistar strain rats (Rattus novergicus). Methods: The study method was true experimental with a posttest-only control group design. Thirty male Wistar rats were randomly divided into five groups: paradoxical sleep deprivation (PSD), total sleep deprivation (TSD), partial sleep deprivation with sleep recovery for five days (PSD+SR), total sleep deprivation with sleep recovery for five days (TSD+SR) and a healthy control group. Data were analysed via one-way ANOVA to determine differences between groups. Result: The results showed the highest level of IL-1β and TNF-α was found in the PSD group. One-way ANOVA analysis showed significant differences (p<0,05) of IL-1β level between PSD and control groups, PSD and PSD+SR groups and PSD and TSD+SR groups; in contrast, the analysis of TNF-α levels showed significant differences (p<0,05) between PSD group to control group, PSD to PSD+SR group and TSD to TSD+SR group. Conclusions: There is an effect of various induction methods of sleep deprivation stress on proinflammatory cytokines (IL-1β and TNF-α).

scholarly journals Kadar Kolesterol Plasma Tikus Wistar pada Pemberiak Ekstrak Etanol dan Heksana dari Daun Gedi Merah (Abelmoschus manihot L.)

Jurnal MIPA ◽  
2015 ◽  
Vol 4 (1) ◽  
pp. 63
Author(s):  
Tanza A. Tubagus
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Plasma Cholesterol ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Amperometric Biosensor ◽  
Male Rats ◽  
Measuring Instrument ◽  
Cholesterol Levels ◽  
One Way Anova

Telah dilakukan penelitian untuk mempelajari pengaruh pemberian ekstrak etanol dan heksana dari daun gedi merah terhadap kadar kolesterol plasma tikus wistar yang diberi pakan aterogenik. Penelitian ini menggunakan 20 ekor tikus jantan, berumur 2-3 bulan dengan berat 130-200 g, yang dibagi dalam 4 kelompok perlakuan secara acak, yaitu kelompok K diberi pakan beras jagung, kelompok K- diberi pakan aterogenik, sedangkan kelompok P1 dan P2 diberi ekstrak etanol dan heksana dengan dosis 20 mg/KgBB. Setiap perlakuan terdiri dari 5 ekor tikus yang dilakukan 5 kali pemeriksaan kadar kolesterol dengan metode biosensor amperometrik dengan menggunakan alat ukur NESCO GCU. Data yang diperoleh dari pemeriksaan dianalisis menggunakan ANOVA satu arah <(0,005). Hasil penelitian menunjukkan bahwa ekstrak heksana dan ekstrak etanol daun gedi merah dengan dosis 20 mg/kgBB dapat menurunkan kadar kolesterol sebesar 72% atau sekitar 12,8 mg/dL dan 14% atau sekitar 2,4 mg/dL, dibandingkan dengan dengan kelompok K-.The research had been done with the purpose were to study the influence of granting the ethanol and hexane extract of red gedi leaves on Wistar rats plasma cholesterol levels which were fed by atherogenics. This study used about 20 male rats, Wistar rats aged 2-3 months with a weight 130-200 g were divided into 4 groups randomly, for the grop of K were fed corn rice, group of K- were fed atherogenics, while the P1 and P2 were given ethanol and hexane extract with a dose of 20 mg/KgBW. Each treatment consisted of 5 rats were performed 5 times cholesterol checks with amperometric biosensor method using a measuring instrument NESCO GCU. Data obtained from the examination were analyzed using one-way ANOVA <(0.005). The results showed that the hexane extract and red gedi leaf ethanol extract at a dose of 20 mg / kg body weight can low down the cholesterol levels by 72% or approximately 12.8 mg / dL and 14%, or about 2.4 mg / dL, compared with the group K -.

scholarly journals UJI EFEK EKSTRAK GEDI MERAH (Abelmoschus manihot L. Medik) TERHADAP KADAR GULA DARAH TIKUS PUTIH JANTAN GALUR WISTAR (Rattus novergicus) YANG DIINDUKSI ALOKSAN

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Friska Adeline ◽  
Jane Wuisan ◽  
Henoch Awaloei
Keyword(s):  
Blood Sugar ◽  
Blood Sugar Level ◽  
Leaf Extract ◽  
White Male ◽  
Male Rats ◽  
Male Rat ◽  
The People ◽  
Wistar Strain ◽  
Medicinal Substances ◽  
Sugar Levels

Abstract: Epidemiological study in Manado indicated that the prevalence of Diabetes Melitus (DM) was 6.1%. It is necessary to do scientific assessment of the various species of plants which are thought containing medicinal substances (phytopharmaca). Based on the information from the people living in the subdistrict Pineleng Minahasa red gedi leaves (Abelmoschus manihot L.Medik ) can be used as herbal treatment for lowering blood sugar level. This study aimed to evaluate the effect of red gedi leaves extract in lowering blood sugar level on white male rat induced by aloksan. This study used 15 white male rats of Wistar strain with diabetic induced by 200 mg/kg BW aloksan intraperitoneal. The rats were randomly divided into 5 groups; each group were given aquadest 2,5 ml/ 200 g BW, insulin Novomix 0,9 U/Kg BW, red gedi leaf extract 1.25 mg/200 g BW, 2.5 mg/200 g BW, and 3.75 mg/200 g BW, once daily for 24 hours. Blood sugar levels were measured at 6th, 12th, 18th, and 24 th hours after treatment by using glucometer. The result showed that red gedi leaf extract can lower blood level in diabetic white male rat induced by aloksan.Keywords: red gedi leaf extract, blood glucose, aloksan, diabeticAbstrak: Pada penelitian epidemiologis di kota Manado didapatkan prevalensi penderita DM 6,1 %. Untuk itu perlu dilakukan pengkajian ilmiah terhadap berbagai spesies tumbuhan yang diduga mengandung zat berkhasiat obat (fitofarmaka). Berdasarkan informasi masyarakat di daerah kecamatan Pineleng kabupaten Minahasa daun Gedi Merah (Abelmoschus manihot L.Medik) dapat dimanfaatkan sebagai penanganan herbal untuk menurunkan kadar gula darah. Penelitian ini bertujuan menguji efektivitas ekstrak daun Gedi Merah (Abelmoschus manihot L. Medik) dalam menurunkan kadar gula darah tikus putih jantan galur Wistar (Rattus norvegicus) yang diinduksi aloksan. Tikus galur Wistar sejumlah 15 dinduksi dengan aloksan intraperitoneal 200 mg/kg BB dan dibagi secara acak dalam 5 kelompok penelitian yaitu kelompok perlakuan dengan aquadest 2,5 ml/200 g BB, insulin Novomix 0,9 U / Kg BB, ekstrak daun gedi merah 1,25 mg/ 200 g BB, 2,5 mg/ 200 g BB dan 3,75 mg/ 200 g BB. Perlakuan diberikan satu kali sehari selama 6 hari. Pengukuran kadar glukosa darah dilakukan pada hari ke 2, 4 dan 6 setelah perlakuan menggunakan glukometer. Hasil penelitian menunjukkan bahwa ekstrak daun gedi merah dapat menurunkan kadar glukosa darah tikus putih jantan diabetes yang diinduksi dengan aloksan.Kata kunci: ekstrak daun gedi merah, glukosa darah, aloksan, diabetes

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