scholarly journals PRODUCTION AND CHARACTERIZATION OF BACTERIAL CELLULOSE FROM KOMAGATAEIBACTER XYLINUS ISOLATED FROM HOME-MADE TURKISH WINE VINEGAR

2021 ◽  
Vol 55 (3-4) ◽  
pp. 243-254
Author(s):  
BURAK TOP ◽  
ERDAL UGUZDOGAN ◽  
NAZIME MERCAN DOGAN ◽  
SEVKI ARSLAN ◽  
NAIME NUR BOZBEYOGLU ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Cytotoxic Effect ◽  
Wound Dressings ◽  
In Vitro Assays ◽  
Water Retention Capacity ◽  
Retention Capacity ◽  
16S Rdna Sequence Analysis ◽  
Wine Vinegar ◽  
Komagataeibacter Xylinus

"In this research, bacterial cellulose (BC) was produced from Komagataeibacter xylinus S4 isolated from home-made wine vinegar (Denizli-Çal) and characterized through morphological and biochemical analyses. K. xylinus was identified by 16S rDNA sequence analysis. The wet (51.8-52.8 g) and dry (0.43-0.735 g) weights of the produced BC were measured. The morphology of cellulose pellicles was examined by scanning electron microscopy (SEM) and a dense nanofiber network was observed. TGA analysis showed that the weight loss in the dehydration step in the BC samples occurred between 50 °C and 150 °C, while the decomposition step took place between 215 °C and 228 °C. Also, the cytotoxic effect, moisture content, water retention capacity and swelling behavior of BC were evaluated. In vitro assays demonstrated that BC had no significant cytotoxic effect. It was found that BC had antibacterial and antibiofilm potential (antibacterial effect>antibiofilm effect). All the results clearly showed that the produced BC can be considered as a safe material for different purposes, such as wound dressings."

scholarly journals Evaluation of the anti-biofilm activities of bacterial cellulose-tannic acid-magnesium chloride composites using an in vitro multispecies biofilm model

2021 ◽  
Author(s):  
Wei He ◽  
Zhaoyu Zhang ◽  
Jing Chen ◽  
Yudong Zheng ◽  
Yajie Xie ◽  
...  
Keyword(s):  
Water Absorption ◽  
Bacterial Cellulose ◽  
Tannic Acid ◽  
Magnesium Chloride ◽  
Chronic Wounds ◽  
Inhibitory Effect ◽  
Wound Dressings ◽  
Retention Capacity ◽  
Biofilm Model

Abstract Chronic wounds are a serious worldwide problem, which are often accompanied by wound infections. In this study, bacterial cellulose (BC)-based composites introduced with tannic acid (TA) and magnesium chloride (BC-TA-Mg) were fabricated for anti-biofilm activities. The prepared composites' surface properties, mechanical capacity, thermal stability, water absorption and retention property, releasing behavior, anti-biofilm activities, and potential cytotoxicity were tested. Results show that TA and MgCl2 particles closely adhered to the nanofibers of BC membranes, thus increasing surface roughness and hydrophobicity of the membranes. While the introduction of TA and MgCl2 did not influence the transparency of the membranes, making it beneficial for wound inspection. BC-TA and BC-TA-Mg composites displayed increased tensile strength and elongation at break compared to pure BC. Moreover, BC-TA-Mg exhibited higher water absorption and retention capacity than BC and BC-TA, suitable for the absorption of wound exudates. BC-TA-Mg demonstrated controlled release of TA and good inhibitory effect on both singly-cultured S. aureus and P. aeruginosa biofilm and co-cultured biofilm of S. aureus and P. aeruginosa. Furthermore, the cytotoxicity grade of BC-TA-6Mg membrane was eligible based on standard toxicity classifications. These indicated that BC-TA-Mg is potential to be used as wound dressings combating biofilms in chronic wounds.

scholarly journals Surface-Modified Bacterial Cellulose with Mercaptosilane as a Multifunctional Platform

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Nayara C. Do Amaral ◽  
Amanda M. Claro ◽  
Gustavo C. Monteiro ◽  
Hernane S. Barud
Keyword(s):  
Bacterial Cellulose ◽  
Elemental Analysis ◽  
Value Added ◽  
High Water ◽  
Water Retention Capacity ◽  
Physical Treatment ◽  
Retention Capacity ◽  
Komagataeibacter Xylinus ◽  
Surface Modified ◽  
Value Added Products

Cellulose synthesized by bacteria has unique properties such as high water retention capacity, biocompatibility, biodegradability and flexibility. Nevertheless, modification of this biomaterial is required in order to obtain multifunctional materials, which may be applied in several high-value added products, as catalytic and cell culture platforms. The surface of bacterial cellulose (BC) can be modified by several approaches, namely: (i) physical treatment by plasma, (ii) adsorption of molecules onto BC surface, and (iii) chemical modification. In this sense, the aim of this study was to modify the BC surface by silanization reaction at room temperature using a mixture of ethanol and water, using two different protocols. Thus, BC membranes synthesized by Komagataeibacter xylinus were modified by adding the thiol (SH) functional group with (3-mercaptopropyl)trimethoxysilane under mild conditions. The produced materials were analyzed by elemental analysis, ATR-FTIR, TGA and SEM, and the successful modification was proven by elemental analysis and SEM. 

scholarly journals Comparison of Bacterial Community Structures in the Rhizoplane of Tomato Plants Grown in Soils Suppressive and Conducive towards Bacterial Wilt

1999 ◽  
Vol 65 (9) ◽  
pp. 3996-4001 ◽  
Author(s):  
Yoshitaka Shiomi ◽  
Masaya Nishiyama ◽  
Tomoko Onizuka ◽  
Takuya Marumoto
Keyword(s):  
Bacterial Wilt ◽  
In Vitro Assays ◽  
Gram Negative Bacteria ◽  
16S Rdna Sequence ◽  
Tomato Plants ◽  
16S Rdna Sequence Analysis ◽  
Bacterial Groups ◽  
Chloroform Fumigation ◽  
Tomato Bacterial Wilt

ABSTRACT It has been reported that the growth of Ralstonia solanacearum is suppressed at the rhizoplane of tomato plants and that tomato bacterial wilt is suppressed in plants grown in a soil (Mutsumi) in Japan. To evaluate the biological factors contributing to the suppressiveness of the soil in three treated Mutsumi soils (chloroform fumigated soil; autoclaved soil mixed with intact Mutsumi soil; and autoclaved soil mixed with intact, wilt-conducive Yamadai soil) infested with R. solanacearum, we bioassayed soil samples for tomato bacterial wilt. Chloroform fumigation increased the extent of wilt disease. More of the tomato plant samples wilted when mixed with Yamadai soil than when mixed with Mutsumi soil. Consequently, the results indicate that the naturally existing population of microorganisms in Mutsumi soil was significantly able to reduce the severity of bacterial wilt of tomato plants. To characterize the types of bacteria present at the rhizoplane, we isolated rhizoplane bacteria and classified them into 22 groups by comparing their 16S restriction fragment length polymorphism patterns. In Yamadai soil a single group of bacteria was extremely predominant (73.1%), whereas in Mutsumi soil the distribution of the bacterial groups was much more even. The 16S rDNA sequence analysis of strains of dominant groups suggested that gram-negative bacteria close to the β-proteobacteria were most common at the rhizoplane of the tomato plants. During in vitro assays, rhizoplane bacteria in Mutsumi soil grew more vigorously on pectin, one of the main root exudates of tomato, compared with those in Yamadai soil. Our results imply that it is difficult for the pathogen to dominate in a diversified rhizobacterial community that thrives on pectin.

scholarly journals Time Dependent Influence of Rotating Magnetic Field on Bacterial Cellulose

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Karol Fijałkowski ◽  
Rafał Rakoczy ◽  
Anna Żywicka ◽  
Radosław Drozd ◽  
Beata Zielińska ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Bacterial Cellulose ◽  
Water Retention ◽  
Rotating Magnetic Field ◽  
Water Retention Capacity ◽  
Water Molecules ◽  
Retention Capacity ◽  
Exposure System ◽  
The Impact ◽  
Water Holding

The aim of the study was to assess the influence of rotating magnetic field (RMF) on the morphology, physicochemical properties, and the water holding capacity of bacterial cellulose (BC) synthetized by Gluconacetobacter xylinus. The cultures of G. xylinus were exposed to RMF of frequency that equals 50 Hz and magnetic induction 34 mT for 3, 5, and 7 days during cultivation at 28°C in the customized RMF exposure system. It was revealed that BC exposed for 3 days to RMF exhibited the highest water retention capacity as compared to the samples exposed for 5 and 7 days. The observation was confirmed for both the control and RMF exposed BC. It was proved that the BC exposed samples showed up to 26% higher water retention capacity as compared to the control samples. These samples also required the highest temperature to release the water molecules. Such findings agreed with the observation via SEM examination which revealed that the structure of BC synthesized for 7 days was more compacted than the sample exposed to RMF for 3 days. Furthermore, the analysis of 2D correlation of Fourier transform infrared spectra demonstrated the impact of RMF exposure on the dynamics of BC microfibers crystallinity formation.

Investigations of Plantago ovata husk Powder as a Disintegrating Agent for Development of Famotidine Tablets

2011 ◽  
Vol 4 (2) ◽  
pp. 1412-1417 ◽  
Author(s):  
Dharmik M Mehta ◽  
P K Shelat ◽  
P B Parejiya ◽  
A J Patel ◽  
B Barot
Keyword(s):  
In Vitro Dissolution ◽  
Water Retention Capacity ◽  
Maize Starch ◽  
Plantago Ovata ◽  
Retention Capacity ◽  
Disintegration Time ◽  
Weight Variation ◽  
Swelling Capacity ◽  
In Vitro Disintegration

 The main objective of this study is to explore development of pharmaceutical excipients from the husk obtained from the seeds of Plantago ovata. Husk shows very good swelling property in water due to the major part of mucilage in it. Since swelling is one of the mechanisms of action of some tablet disintegrants, it is thought that the husk powder of Plantago ovata would be able to act as a tablet disintegrant. The powder obtained from the Plantago ovata husk was characterized for micromeritical properties, swelling capacity, hydration capacity, LOD, pH, particle size, foreign particles, ash value and microbial limit tests. Its disintegrant ability in comparison with maize starch was investigated by preparing famotidine tablets via the direct compression method. It was also compared with three marketed tablets of famotidine. The Plantago ovata husk powder, however, showed superior flow, swelling capacity as well as water retention capacity than maize starch. The tablets were characterized for hardness, friability, weight variation, in vitro disintegration study and in vitro dissolution study. The optimized batch F2C comprising of 10% of the Plantago ovata husk powder showed a 15 seconds disintegration time, which was significantly less than tablets prepared from maize starch as well as all three market preparations. Tablets from batch F2C were submitted for short term stability studies and exhibited stable characteristics.

scholarly journals Photocrosslinkable Nanofibrous Asymmetric Membrane Designed for Wound Dressing

Polymers ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 653 ◽  
Author(s):  
Patrícia Alves ◽  
Marta Santos ◽  
Sabrina Mendes ◽  
Sónia P. Miguel ◽  
Kevin D. de Sá ◽  
...  
Keyword(s):  
Wound Dressing ◽  
Healing Process ◽  
Protective Layer ◽  
Fibroblast Cell ◽  
Wound Dressings ◽  
In Vitro Assays ◽  
Asymmetric Membrane ◽  
Electrospinning Technique ◽  
Asymmetric Membranes

Recently, the biomedical scientists who are working in the skin regeneration area have proposed asymmetric membranes as ideal wound dressings, since they are able to reproduce both layers of skin and improve the healing process as well as make it less painful. Herein, an electrospinning technique was used to produce new asymmetric membranes. The protective layer was composed of a blending solution between polycaprolactone and polylactic acid, whereas the underlying layer was comprised of methacrylated gelatin and chitosan. The chemical/physical properties, the in vitro hemo- and biocompatibility of the nanofibrous membranes were evaluated. The results obtained reveal that the produced membranes exhibited a wettability able to provide a moist environment at wound site. Moreover, the membranes’ hemocompatibility and fibroblast cell adhesion, spreading and proliferation at the surface of the membranes were also noticed in the in vitro assays. Such results highlight the suitability of these asymmetric membranes for wound dressing applications.

scholarly journals Scaffolds for Chondrogenic Cells Cultivation Prepared from Bacterial Cellulose with Relaxed Fibers Structure Induced Genetically

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 1066 ◽  
Author(s):  
Paulina Jacek ◽  
Marcin Szustak ◽  
Katarzyna Kubiak ◽  
Edyta Gendaszewska-Darmach ◽  
Karolina Ludwicka ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bacterial Cellulose ◽  
Spatial Organization ◽  
Three Dimensional ◽  
In Vitro Assays ◽  
Glycosaminoglycan Synthesis ◽  
Nano Cellulose ◽  
Komagataeibacter Hansenii

Development of three-dimensional scaffolds mimicking in vivo cells’ environment is an ongoing challenge for tissue engineering. Bacterial nano-cellulose (BNC) is a well-known biocompatible material with enormous water-holding capacity. However, a tight spatial organization of cellulose fibers limits cell ingrowth and restricts practical use of BNC-based scaffolds. The aim of this study was to address this issue avoiding any chemical treatment of natural nanomaterial. Genetic modifications of Komagataeibacter hansenii ATCC 23769 strain along with structural and mechanical properties characterization of obtained BNC membranes were conducted. Furthermore, the membranes were evaluated as scaffolds in in vitro assays to verify cells viability and glycosaminoglycan synthesis by chondrogenic ATDC5 cells line as well as RBL-2H3 mast cells degranulation. K. hansenii mutants with increased cell lengths and motility were shown to produce BNC membranes with increased pore sizes. Novel, BNC membranes with relaxed fiber structure revealed superior properties as scaffolds when compared to membranes produced by a wild-type strain. Obtained results confirm that a genetic modification of productive bacterial strain is a plausible way of adjustment of bacterial cellulose properties for tissue engineering applications without the employment of any chemical modifications.

scholarly journals Chitosan Reinforced with Kenaf Nanocrystalline Cellulose as an Effective Carrier for the Delivery of Platelet Lysate in the Acceleration of Wound Healing

Polymers ◽  
2021 ◽  
Vol 13 (24) ◽  
pp. 4392
Author(s):  
Payal Bhatnagar ◽  
Jia Xian Law ◽  
Shiow-Fern Ng
Keyword(s):  
Wound Healing ◽  
In Vitro Release ◽  
Nanocrystalline Cellulose ◽  
Platelet Lysate ◽  
Moisture Loss ◽  
Water Retention Capacity ◽  
Retention Capacity ◽  
Treatment Of Wounds ◽  
Kenaf Bast Fibers

The clinical use of platelet lysate (PL) in the treatment of wounds is limited by its rapid degradation by proteases at the tissue site. This research aims to develop a chitosan (CS) and kenaf nanocrystalline cellulose (NCC) hydrogel composite, which intend to stabilize PL and control its release onto the wound site for prolonged action. NCC was synthesized from raw kenaf bast fibers and incorporated into the CS hydrogel. The physicochemical properties, in vitro cytocompatibility, cell proliferation, wound scratch assay, PL release, and CS stabilizing effect of the hydrogel composites were analyzed. The study of swelling ratio (>1000%) and moisture loss (60–90%) showed the excellent water retention capacity of the CS-NCC-PL hydrogels as compared with the commercial product. In vitro release PL study (flux = 0.165 mg/cm2/h) indicated that NCC act as a nanofiller and provided the sustained release of PL compared with the CS hydrogel alone. The CS also showed the protective effect of growth factor (GF) present in PL, thereby promoting fast wound healing via the formulation. The CS-NCC hydrogels also augmented fibroblast proliferation in vitro and enhanced wound closures over 72 h. This study provides a new insight on CS with renewable source kenaf NCC as a nanofiller as a potential autologous PL wound therapy.

scholarly journals Superabsorbent crosslinked bacterial cellulose biomaterials for chronic wound dressings

2020 ◽  
Author(s):  
Daria Ciecholewska-Juśko ◽  
Anna Żywicka ◽  
Adam Junka ◽  
Radosław Drozd ◽  
Peter Sobolewski ◽  
...  
Keyword(s):  
Sodium Bicarbonate ◽  
Bacterial Cellulose ◽  
Chronic Wounds ◽  
Ammonium Bicarbonate ◽  
Wound Dressings ◽  
Ex Situ ◽  
Water Release ◽  
Water Capacity ◽  
Disodium Phosphate

AbstractIn this work, we present novel ex situ modification of bacterial cellulose (BC) polymer, that significantly improves its ability to absorb water after drying. The method involves a single inexpensive and easy-to-perform process of BC crosslinking, using citric acid along with catalysts, such as disodium phosphate, sodium bicarbonate, ammonium bicarbonate or their mixtures. In particular, the mixture of disodium phosphate and sodium bicarbonate was the most promising, yielding significantly greater water capacity (over 5 times higher as compared to the unmodified BC) and slower water release (over 6 times as compared to the unmodified BC). Further, our optimized crosslinked BC had over 1.5x higher water capacity than modern commercial dressings dedicated to highly exuding wounds, while exhibiting no cytotoxic effects against fibroblast cell line L929 in vitro. Therefore, our novel BC biomaterial may find application in super-absorbent dressings, designed for chronic wounds with imbalanced moisture level.

scholarly journals Crystal and Supramolecular Structure of Bacterial Cellulose Hydrolyzed by Cellobiohydrolase from Scytalidium Candidum 3C: A Basis for Development of Biodegradable Wound Dressings

Materials ◽  
2020 ◽  
Vol 13 (9) ◽  
pp. 2087 ◽  
Author(s):  
Lyubov A. Ivanova ◽  
Konstantin B. Ustinovich ◽  
Tamara V. Khamova ◽  
Elena V. Eneyskaya ◽  
Yulia E. Gorshkova ◽  
...  
Keyword(s):  
Wound Healing ◽  
Specific Surface ◽  
Bacterial Cellulose ◽  
Supramolecular Structure ◽  
Polymer Network ◽  
Fold Increase ◽  
Wound Dressings ◽  
Submicron Particles

The crystal and supramolecular structure of the bacterial cellulose (BC) has been studied at different stages of cellobiohydrolase hydrolysis using various physical and microscopic methods. Enzymatic hydrolysis significantly affected the crystal and supramolecular structure of native BC, in which the 3D polymer network consisted of nanoribbons with a thickness T ≈ 8 nm and a width W ≈ 50 nm, and with a developed specific surface SBET ≈ 260 m2·g−1. Biodegradation for 24 h led to a ten percent decrease in the mean crystal size Dhkl of BC, to two-fold increase in the sizes of nanoribbons, and in the specific surface area SBET up to ≈ 100 m2·g−1. Atomic force and scanning electron microscopy images showed BC microstructure “loosening“after enzymatic treatment, as well as the formation and accumulation of submicron particles in the cells of the 3D polymer network. Experiments in vitro and in vivo did not reveal cytotoxic effect by the enzyme addition to BC dressings and showed a generally positive influence on the treatment of extensive III-degree burns, significantly accelerating wound healing in rats. Thus, in our opinion, the results obtained can serve as a basis for further development of effective biodegradable dressings for wound healing.

Sign in / Sign up

Export Citation Format

Share Document