scholarly journals THE EFFECT OF INTERNAL ENVIRONMENT OF ANIMALS ON PROPERTIES OF PROBIOTIC BACTERIA

2014 ◽  
Vol 19 ◽  
pp. 89-94
Author(s):  
V. O. Aheyev
Keyword(s):  
Bacillus Subtilis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Biological Properties ◽  
Antagonistic Activity ◽  
Probiotic Bacteria ◽  
Internal Environment ◽  
Size And Shape

The paper depicts the results of the study of influence of internal environment of animals on preservation of initial biological properties of probiotic bacteria. The increased by 10–15 % antagonistic activity was observed in Bacillus subtilis 44-p strain after its translocation to the laboratory rabbits while for B. subtilis B3 the changes in the morphology of colonies (size and shape) were noticed. Nevertheless, the properties of the studied lactic acid bacteria after exposition to the internal environment of the macroorganism remain unchanged.

scholarly journals Probiotic Bifunctionality of Bacillus subtilis—Rescuing Lactic Acid Bacteria from Desiccation and Antagonizing Pathogenic Staphylococcus aureus

2019 ◽  
Vol 7 (10) ◽  
pp. 407 ◽  
Author(s):  
Hadar Kimelman ◽  
Moshe Shemesh
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pathogenic Bacteria ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Bacterium ◽  
Probiotic Bacteria ◽  
Mammalian Host ◽  
Beneficial Effects

Live probiotic bacteria obtained with food are thought to have beneficial effects on a mammalian host, including their ability to reduce intestinal colonization by pathogens. To ensure the beneficial effects, the probiotic cells must survive processing and storage of food, its passage through the upper gastrointestinal tract (GIT), and subsequent chemical ingestion processes until they reach their target organ. However, there is considerable loss of viability of the probiotic bacteria during the drying process, in the acidic conditions of the stomach, and in the high bile concentration in the small intestine. Bacillus subtilis, a spore-forming probiotic bacterium, can effectively maintain a favorable balance of microflora in the GIT. B. subtilis produces a protective extracellular matrix (ECM), which is shared with other probiotic bacteria; thus, it was suggested that this ECM could potentially protect an entire community of probiotic cells against unfavorable environmental conditions. Consequently, a biofilm-based bio-coating system was developed that would enable a mutual growth of B. subtilis with different lactic acid bacteria (LAB) through increasing the ECM production. Results of the study demonstrate a significant increase in the survivability of the bio-coated LAB cells during the desiccation process and passage through the acidic environment. Thus, it provides evidence about the ability of B. subtilis in rescuing the desiccation-sensitive LAB, for instance, Lactobacillus rhamnosus, from complete eradication. Furthermore, this study demonstrates the antagonistic potential of the mutual probiotic system against pathogenic bacteria such as Staphylococcus aureus. The data show that the cells of B. subtilis possess robust anti-biofilm activity against S. aureus through activating the antimicrobial lipopeptide production pathway.

scholarly journals INFLUENCE OF PROBIOTIC BACTERIA ON THE TENDENCY AND INTENSITY OF MICROBIOLOGICAL PROCESSES DURING ENSILAGE OF CORN GREEN CROP

2021 ◽  
Vol 32 ◽  
pp. 58-66
Author(s):  
N. O. Kravchenko ◽  
O. M. Dmytruk ◽  
N. M. Furs
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Raw Materials ◽  
Ammonia Nitrogen ◽  
Antagonistic Activity ◽  
Probiotic Bacteria ◽  
Microbiological Processes ◽  
Combined Use ◽  
Taxonomic Groups

Objective. To study the influence of probiotic bacteria on the tendency and intensity of microbiological processes during ensilage of corn green crop. Methods. Microbiological. zootechnical, statistical. Results. The content of lactic acid in the silage obtained with two strains of Lactobacillus plantarum КT-L18/1 and Bacillus subtilis ВРТ-В1 is higher than in the variants with monocultures of these strains (0.2 % and 1 %, respectively). Enhancement of antagonistic activity upon the combination of the studied strains of probiotic bacteria of different taxonomic groups is registered only for individual test cultures, in particular for Pseudomonas aeruginosa. Treatment of corn green crop under the laboratory conditions with the studied probiotic bacteria in both monocultures and two or three strain combinations at Day 30 of fermentation helped to improve the microbiological state of the ensiled mass compared to the control variant (without introduction of microorganisms). The combined use of L. plantarum КT-L18/1 and B. subtilis ВРТ-В1 revealed a 53 % higher number of lactic acid bacteria compared to the variant with the treatment of raw materials with one strain of B. subtilis 44-p. The highest content of lactic acid and its best ratio with acetic acid was found in the variant with the combined use of L. plantarum КТ-L18/1 and B. subtilis ВРТB1. The lowest content of ammonia nitrogen was registered in the variant with the combined use of two strains of lactic acid bacteria (7 %), slightly higher — in the variant with the combined use of strains of lactic acid bacteria and B. subtilis (7.75 %). Conclusion. The use of probiotic strains of bacteria in the ensilage of corn green crop helps to improve microbiological processes during the maturation of silage. The greatest increase in the number of lactic acid bacteria against the background of the lowest number of fungi and yeast is registered upon co-application of two studied strains of probiotic bacteria of different taxonomic groups, which indicates better aerobic stability of feed. Due to the use of a combination of strains of L. plantarum КT-L18/1 and B. subtilis ВРТ-B1 in the treatment of corn green crop in the laboratory experiments, the accumulation of the highest content of lactic acid, its optimal ratio to acetic acid and reducing ammonia nitrogen, which contributes to better retention of nutrients in the feed was shown.

DEVELOPMENT OF A MULTIWELL ANTAGONISTIC ACTIVITY ASSAY FOR THE DETECTION OF BACTERIOCIN PRODUCTION BY LACTIC ACID BACTERIA

2009 ◽  
Vol 17 (1) ◽  
pp. 32-45 ◽  
Author(s):  
KAOUTAR YAAKOUBI ◽  
NOREDDINE BENKERROUM ◽  
FLORENT WIOROWSKI ◽  
FRANÇOISE SANSON ◽  
JULIEN HAYDERSAH ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Antagonistic Activity ◽  
Bacteriocin Production ◽  
Activity Assay

scholarly journals Complete Genome Sequence of Lactobacillus reuteri Byun-re-01, Isolated from Mouse Small Intestine

2018 ◽  
Vol 7 (17) ◽  
Author(s):  
Dongjun Kim ◽  
Mun-ju Cho ◽  
Seungchan Cho ◽  
Yongjun Lee ◽  
Sung June Byun ◽  
...  
Keyword(s):  
Small Intestine ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Lactobacillus Reuteri ◽  
Probiotic Bacteria ◽  
Content Type ◽  
Mouse Small Intestine

Lactic acid bacteria (LAB) are generally recognized as safe (GRAS) and serve as probiotic bacteria when consumed in adequate amounts. Here, we report the complete genome sequence of Lactobacillus reuteri Byun-re-01, isolated from mouse small intestine.

Spray-Drying of Bacteriocin-Producing Lactic Acid Bacteria

1999 ◽  
Vol 62 (7) ◽  
pp. 773-777 ◽  
Author(s):  
GIANLUIGI MAURIELLO ◽  
MARIA APONTE ◽  
ROSAMARIA ANDOLFI ◽  
GIANCARLO MOSCHETTI ◽  
FRANCESCO VILLANI
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Cell Survival ◽  
Spray Drying ◽  
Antagonistic Activity ◽  
Cellular Damage ◽  
Bacteriocin Activity ◽  
Lactobacillus Curvatus ◽  
Significant Difference ◽  
Inhibitory Spectrum

Cell survival, cellular damage, and antagonistic activity were investigated after spray-drying of four bacteriocin-producing strains of lactic acid bacteria: Lactococcus lactis subsp. lactis 140, isolated from natural whey culture and producing a narrow-inhibitory spectrum bacteriocin); L. lactis subsp. lactis G35, isolated from pizza dough and producing nisin; Lactobacillus curvatus 32Y and Lactobacillus sp. 8Z, isolated from dry sausages. Trials were performed with bacteria suspended in skimmed milk or directly grown in whey. Three air temperatures at the inlet of the drier (160, 180, and 200°C) and three flow rates (10, 13, and 17 ml/min) were assayed. Cell viability and bacteriocin activity of the dried materials were determined immediately after the process and after 5, 15, 30, and 60 days of storage at 4°C. There was no significant difference between the two feeding suspensions in cell survival, always decreasing with the increase of inlet-air temperature. No loss of bacteriocin activity was detected in reconstituted powders, nor was any loss of ability to produce bacteriocin found after drying. Investigations of sensitivity to NaCl revealed only temporary damage to dried bacteria. During storage for 2 months at 4°C, all samples, but mainly the lactococcal strains, displayed a gradual decrease in cell survival. Bacteriocin activity remained at the same level, allowing powders to be considered as effective biopreservatives.

Pro-/Anti-Inflammatory Food Supplements

2020 ◽  
pp. 142-168
Author(s):  
Hüseyin Eseceli
Keyword(s):  
Immune Response ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Food Supplements ◽  
Gastrointestinal System ◽  
Probiotic Bacteria ◽  
Immune Systems ◽  
Positive Effects ◽  
Endogenous Defense ◽  
Probiotic Effect

Probiotics are living microorganisms, meaning “for life” and consist of two parts, “pro” and “biota,” and prebiotics are indigestible carbohydrates that increase the number and activities of colon bacteria and the effectiveness of probiotics. Probiotic consumption has been reported to have many positive effects, such as increasing immune response, balancing the colony, and increasing endogenous defense capacity of cells. The aim of prebiotics is to grow probiotic bacteria, thus improving the gastrointestinal and immune systems. Recent studies have shown that probiotics may have an impact on gastrointestinal system diseases. It has been shown to stimulate the proliferation of beneficial microorganisms in prebiotics and assist in the probiotic effect. The most commonly used probiotics and prebiotics are lactic acid bacteria that are types of Lactobacillus and Bifidobacterium. Prebiotics are naturally present in nutrients. Probiotics and prebiotics are used to prevent many diseases.

scholarly journals ISOLATION, SCREENING, AND CHARACTERIZATION OF PROBIOTICS (LACTIC ACID BACTERIA) ANTAGONISTIC AGAINST Candida albicans

2017 ◽  
Vol 4 (2) ◽  
pp. 263
Author(s):  
Ida Ayu Ketut Ariningsih ◽  
Yan Ramona ◽  
Nyoman Semadi Antara
Keyword(s):  
Candida Albicans ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Reproductive Tract ◽  
Antagonistic Activity ◽  
Low Ph ◽  
Production Test ◽  
Ph Conditions ◽  
High Level

Candidacies in female reproductive tract are mainly caused by Candida albicans. This infection often causes serious problems, particularly on their reproductive tract (genital part). Until recently, control of this infection has relied on the use of antibiotics. However due to numerous bad side effects of antibiotics, lactic acid bacteria have been proposed as an alternative method to control the growth of Candida albicans. Therefore, this research was aimed to isolate, screen, and characterize lactic acid bacterial isolates (LAB) antagonistic against Candida albicans (the causative agent of candidacies infection in reproductive tract of human). LABs were isolated from various fermented foods, such as tape ketan and kimchi. Isolation of LABs was conducted by applying dilution and spread plate method on MRS agar medium supplemented with BCP indicator to distinguish LABs from non acid-producing bacteria. Colonies with indication to produce acid were screened for antagonistic activity against C. albicans on MRS agar and followed by characterization of those isolates (Gram stain, catalase production test, oxydase production, gas production test, resistance test to low pH conditions and to high level of NaDC (sodium deoxicolic), and test for ability to convert colic acid (CA) into deoxicolic acid (DCA)). The results showed that 46 LAB isolates were successfully isolated from samples of tape ketan and kimchi. Among those, 7 isolates showed antagonistic activity against C. albicans in in vitro tests. All these 7 candidates were also found to be resistance to low pH conditions (up to pH 2) and to high level of NaDC (up to 0.6 mM). Four most potential isolates were further testes for ability to convert colic acid into deoxycolic acid and none showed positive result, indicating that they all showed initial potential and safe for future human probiotic development (especially to be used to treat patients infected by C. albicans).

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