scholarly journals The Utilization of Sweet Potatoes as Prebiotics on The Performance of Lactobacillus sp. in The Vanamei Shrimp Digestion (Litopenaeus vannamei)

Author(s):  
Maria Theresia ◽  
Siti Aslamyah ◽  
Sriwulan Sriwulan

The use of probiotics (Lactobacillus  sp.) in feed is one alternative that is done to improve immunostimulants, growth stimulants, and can be used as a balance of microorganisms in digestion. This study aimed to evaluate the growth performance of vanamei shrimp fed with the addition of Lactobacillus  sp. with sweet potatoes in feed. This study used a completely randomized design (CRD) with 4 treatments and 3 replications. The treatment was by feeding with different sweet potato concentrations: A) The dose of sweet potato in feed was 0% (as control); B) The dose of sweet potato in feed was 10%; C) 1The dose of sweet potato in feed was 15%; and D) The dose of sweet potato in feed was 20%. Data were analyzed using variance analysis (ANOVA) and continued with W-Tuckey's further test. The research parameters were digestive enzyme activity, nutrient digestibility, and growth. The results of the variance analysis showed the addition of various sweet potato concentrations with Lactobacillus sp. has no significant effect (p> 0.05) of the growth rate. The specific growth rate of vannamei shrimp ranges from 3.06 to 3.23% / day. Based on the results of the analysis on the utilization of sweet potatoes as a prebiotic on the performance of Lactobacillus sp. in the vannamei shrimps (Litopenaeus vannamei) digestive tract can be concluded that the growth performance does not have a significant effect after being fed with the addition of Lactobacillus sp. and sweet potatoes in feed. Keywords: Enzymes, Digestion, Growth, Lactobacillus sp., Prebiotic, Probiotic.

2021 ◽  
Vol 1 (1) ◽  
pp. 16-28
Author(s):  
Mahfud Akbar ◽  
Muhammad Junaidi ◽  
Fariq Azhar

Research on the effect of probiotic dosing on the growth of vaname shrimp (Litopenaeus vannamei). This research was conducted for 45 days from 26 August to 14 October 2019 which took place at UD Kolbun Nanda Mumbul Sari Village, Bayan District, North Lombok Regency, West Nusa Tenggara (NTB). This study used the CRD method (completely randomized design) with 5 treatments and 3 replications. The container used in this study was a sterofome with p 82 x50x38 cm, so that the area of ​​the container used was 155,800 cm3 and the volume of water in the container was 50 liters. The containers used were 15 sterofom units with 5 treatments and 3 replications, where in treatment one (P1) used probiotics at a dose of 0.2 ml / l / week, treatment two (P2) used probiotics at a dose of 0.4 ml / l / week, treatment three (P3) used probiotics at a dose of 0.6 ml / l / week, treatment four (P4) used probiotics at a dose of 0.8 ml / l / week, and treatment five (P5) used probiotics at a dose of 1 ml / l / week. Parameters observed during the study included growth rate, survival rate (SR), and feed conversion ratio (FCR) of vanname shrimp. The results showed that the administration of probiotics with different doses did not have a significant effect on growth in absolute weight and growth in daily weight. However, it had a significantly different effect on the survival rate and feed conversion ratio.


2021 ◽  
Vol 10 (2) ◽  
pp. 252-258
Author(s):  
Pandu Abdi Perdana ◽  
Salnida Yuniarti Lumbessy ◽  
Bagus Dwi Hari Setyono

Penelitian ini bertujuan untuk menganalisa pengaruh pengkayaan pakan Artemia sp. dengan Chaetoceros sp. terhadap performa pertumbuhan larva udang vaname (Litopenaeus vannamei). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan menggunakan empat (4) perlakuan perbedaan dosis Chaetoceros untuk pengkayaan Artemia sp., yaitu A (pakan alami Artemia sp. tanpa pengkayaan Chaetoceros) sebagai perlakuan kontrol, B (dosis pengkayaan Chaetoceros 1,5 x 105 sel/ind), C (dosis pengkayaan Chaetoceros 2,5 x 105  sel/ind) dan D (dosis pengkayaan Chaetoceros 3,5 x 105 sel/ind), masing-masing perlakuan dengan 4 kali ulangan. Data dianalisis menggunakan analysis of variance (ANOVA) pada taraf nyata 5% dengan selang kepercayaan 95% dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa pengkayaan pakan alami Artemia sp. dengan Chaetoceros dapat meningkatkan laju pertumbuhan panjang mutlak dan spesifik larva udang vaname tetapi tidak mempengaruhi tingkat kelangsungan hidupnya, dimana perlakuan dosis Chaetoceros 3,5 x 105 sel/ind memberikan laju pertumbuhan panjang mutlak dan spesifik  larva udang vaname yang lebih baik, yaitu berturut-turut  5,45 mm, dan 8,60%/hari. Pengkayaan pakan alami Artemia sp. dengan Chaetoceros sp. dapat meningkatkan nilai protein pakan larva udang vanname hingga mencapai 66 %. This study aims to analyze the effect of Artemia sp. with Chaetoceros sp. on the growth performance of vaname shrimp larvae (Litopenaeus vannamei). This study used a completely randomized design (CRD) using four (4) different treatments of Chaetoceros Dose for Artemia sp. Enrichment, namely  A (natural feed of Artemia sp. without Chaetoceros Enrichment) as control treatment, B (Chaetoceros sp. Enrichment dose 1,5 × 105 cells / ind), C (Chaetoceros enrichment dose 2,5 × 105 cells / ind) and D (Chaetoceros enrichment dose 3,5 × 105 cells / ind) , each treatment with 4 replications. The data were analyzed using analysis of variance (ANOVA) at a significant level of 5% with a 95% confidence interval and continued with the Duncan test. The results showed that enrichment of natural feed for Artemia sp. with Chaetoceros The growth rate of absolute and specific length of vannamei shrimp larvae but does not affect the survival rate, where the Chaetoceros treatment dose of 3,5 × 105 cells / ind gives the absolute and specific length growth rate of the vaname shrimp larvae. better, namely 5.45 mm, and 8.60% / day, respectively. Natural food enrichment of Artemia sp. with Chaetoceros sp. can increase the protein value of vanname shrimp larvae feed by up to 66%


2018 ◽  
Vol 10 (1) ◽  
pp. 131-137 ◽  
Author(s):  
Siti Samiyarsih ◽  
Juwarno Juwarno ◽  
Juni Safitri Muljowati

Anatomical characters can be used as instructions to the structural resistance of plants to pathogen attack. Various pathogens attack sweet potato plants, such as the Sphaceloma batatas fungus that causes scurvy disease (scab). The aims of this research t test the structural resistance of sweet potato plants based on leaf anatomical character and intensity of disease attack due to the inoculated of S. batatas. The research was conducted from June to November 2016, using the Completely Randomized Design Experimental (RAL) method with a factorial pattern. The first factor was ten sweet potato cultivars and the second factor was the inoculum of S. batatas fungus each treatment with five replications. The character of leaf anatomy observed was thick of cuticle, thick of mesophyll, size and number of stomata and number of trichomes per 1 mm2 leaf area. Based on the research result, it was concluded that the inoculation of the fungus of S. batatas caused the decrease of stomata length and width on ten sweet potato cultivars. The highest intensity of disease attack was 14.33% and correlated with stomata length (r = 0.49). The anatomical structural resistance to scurvy can be used as a basis for determining crosses for obtaining superior sweet potato cultivars. The benefit of the research is to advise the community to cultivate sweet potatoes that have structural resistance to scurvy, such as cuticle and thick mesophyll, high trichomata density.


2020 ◽  
Vol 7 (2) ◽  
pp. 39
Author(s):  
Rani Andrianti Ekaputri ◽  
Muhammad Arief ◽  
Boedi Setya Rahardja

Litopenaeus vannamei is a leading commodity whose economic value. Targets of vannamei’s production increasing every year. One of important factor in the success of shrimp farming is feed. Research about feed fish directed to the creation of feed fish whose economical and safe for the environment, therefore is required an efforts to provide alternative feed through feed supplement. One of the material that can be supplemented in feed is chitosan. Chitosan can increase the specific growth rate and protein retention of vannamei. This study aims to determine the effect of chitosan supplementation in commercial feed for specific growth rate and protein retention of Litopenaeus vannamei. The research method used was completely randomized design (RAL) and performed with five treatments chitosan supplementation (0%, 1%, 2%, 3% dan 4%) experimental animal used was ten shrimps as five replication in each treatment. The main parameters were observed are specific growth rate and protein retention of vaname. Supporting parameters were observed is water quality. The results showed that chitosan supplementation in commercial feed for 30 days of maintenance give an effect on the specific growth rate and protein retention of vaname. The highest specific growth rate was obtained in treatment with 4% chitosan supplementation that not significantly different with treatment 3% chitosan supplementation. Protein retention was incresed in treatment with 4% chitosan supplementation that not significantly different with treatment 3% chitosan supplementation.


2016 ◽  
Vol 6 (1) ◽  
pp. 83-93
Author(s):  
Wida Lesmanawati ◽  
Widanarni ◽  
Sukenda

Synbiotic application shows better results compared to the single use of probiotics or prebiotics. SKT-b bacteria have been shown to act as probiotics for aquatic animals, as well as sweet potatoes that are known to act as prebiotics. This study aims to examine the synbiotic potential of SKT-b probiotic bacteria and oligosaccharide extract from sweet potato in improving the growth performance of vaname shrimp. Shrimp were treated with synbiotic feed with different prebiotic concentrations of 1% (Pro + Pre 1%), 2% (Pro + Pre 2%) and 3% (Pro + Pre 3%). The treated food was given to shrimp (weight ± 1.9 g) for 30 days. Oligosaccharides extracted from sweet potato can act as a prebiotic which increases the bacterial population in vaname shrimp’s intestine. The application of synbiotics can improve the growth performance of vaname shrimp including the addition of body weight, feed efficiency, digestive enzyme activity, protein retention and body fat of shrimp. The treatment of Pro + Pre 2% and Pro + Pre 3% shows better growth performance of vaname shrimp.


Author(s):  
Abdul Rakhfid ◽  
Wa Ode Halida ◽  
Rochmady Rochmady ◽  
Fendi Fendi

The study aimed to determine the growth and survival of Litopenaeus vannamei shrimp with the application of probiotics to different stocking densities.  The study was conducted from May to June 2018 at the Balai Benih Ikan Pantai (BBIP) Ghonebalano, Duruka District, Muna Regency, using a Completely Randomized Design (CRD) with three levels of solid stocking treatment namely treatment A is 18 individu per container, treatment B is 24  individu per container and treatment C is 30  individu per container with three replications.  Data analysis using Variance Analysis (ANOVA) at the confidence level of 95% (α 0.05).  The results showed that the highest daily growth was obtained at 24 individual densities of 5.88% per day, compared to 18 individual densities of 5.86% per day and 30 individual densities of 5.74% per day.  The highest absolute growth is obtained at 24 individual density of 2.43 g per individu, then 18 individual density of 2.15 g per individual, and the lowest at 30 individual density of 2.02 g per individual.  Survival at a density of 18 individual and a density of 24 individual at 88.89%, while a 30-ind density of 86.67%.  Analysis of variance (α 0.05) showed that the application of probiotics to different stocking densities had no significant effect on the daily growth rate of vannamei shrimp (p<0.939), absolute growth of vannamei shrimp (p<0.080), and survival of vannamei shrimp (p<0.744).


2021 ◽  
Vol 21 (1) ◽  
pp. 11-22
Author(s):  
Surianti Surianti ◽  
Fitratul Muaddama ◽  
Wahyudi Wahyudi ◽  
Sri Wahyuni Firman

This study aims to determine the effect of the dose of fermented rice bran using Lactobacillus sp. on growth performance and enzyme activity in tilapia. This research was conducted in Sidenreng Rappang Regency. The test animal used was tilapia larvae measuring 1.17 g. The maintenance container is a tarp pool with a size of 1 m x 1 m x 1 m filled with fresh water as much as 85 L. The study was designed using a completely randomized design consisting of four treatments and three replications, thus there were 12 experimental units. The treatment was determined based on the dose of fermented rice bran using Lactobacillus sp. which differ, namely 0, 10, 15 and 20%. The test feed was given to tilapia larvae, which were stocked with 20 fish per container for 60 days of rearing. The specific growth rate data of tilapia were analysed using analysis of variance (ANOVA) and enzyme activity was analysed descriptively based on the viability of tilapia. The results showed that tilapia fed with 20% fermented rice bran had a significant effect on the specific growth rate, protease and amylase enzyme activity. The optimal dosage of fermented rice bran using Lactobacillus sp. is 15-20%.


Author(s):  
S Surianti ◽  
A Aslamyah ◽  
W Wahyudi

ABSTRACTThis study aims to determine the effect of the use of fermented tofu dregs using mix microorganisms on the growth of vaname shrimp (Litopenaeus vannamei). Test animals used in this study were 22-day-old vaname shrimp (PL 22) with an initial weight of ± 0.09 g. This study uses a completely randomized design (CRD) consisting of 4 treatments and 3 replications to obtain 12 experimental units, namely PA (5% fermented tofu waste) PB (10% fermented tofu waste) PC (15% fermented tofu waste) PD (20% fermented tofu pulp). The results showed that the treatment of various doses of fermented tofu dregs using Microorganism Mix significantly affected the growth rate and survival but did not affect the level of consumption of vaname shrimp feed. 15% fermented tofu pulp dosage is the best treatment for absolute growth, survival and consumption level of vaname shrimp feed Keywords: Tofu waste, mixed microorganisms, vaname shrimp, absolute growth, survival ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh penggunaan ampas tahu terfermentasi menggunakan mikroorganisme mix terhadap pertumbuhan udang vaname (Litopenaeus vannamei). Hewan uji yang digunakan dalam penelitian ini adalah udang vaname umur 22 hari (PL 22) dengan bobot awal ± 0,09 g. Penelitian ini menggunakan rancangan acak lengkap (RAL) yang terdiri dari 4 perlakuan dan 3 kali ulangan sehingga diperoleh 12 unit percobaan, yakni PA (5 % ampas tahu terfermentasi) PB (10 % ampas tahu terfermentasi) PC (15 % ampas tahu terfermentasi) PD (20 % ampas tahu terfermentasi). Hasil penelitian menunjukkan perlakuan berbagai dosis ampas tahu terfermentasi menggunakan Mikroorganisme Mix berpengaruh nyata terhadap laju pertumbuhan dan sintasan tetapi tidak berpengaruh terhadap tingkat konsumsi pakan udang vaname. Dosis ampas tahu terfermentasi sebanyak 15% merupakan perlakuan terbaik untuk pertumbuhan mutlak, kelangsungan hidup dan tingkat konsumsi pakan udang vanameKata kunci : Ampas tahu, mikroorganisme mix, udang vaname, pertumbuhan mutlak, kelangsungan hidup


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 158-159
Author(s):  
Hailey Wooten ◽  
Hwanhee Kim ◽  
Amanda Rakhshandeh ◽  
Anoosh Rakhshandeh

Abstract We previously found that i.m. injection of GRA, similar to in-feed antibiotics, improves measures of intestinal integrity, nutrient digestibility, and overall growth performance in nursery pigs. In addition, we have demonstrated that the mitigating effects of GRA on the stress of weaning is predominantly done by subsiding the inflammatory response that often accompanies early weaning. The objective of the current study was to explore the most practical methods for delivering GRA to newly weaned pigs. One-hundred and sixty-seven PIC pigs were weaned at 25 ±1.0 days of age (BW 7 ±1.2 kg), and assigned to fourteen treatments (3-4 pigs /pen; 3 pens/treatment). Seven treatments were tested within two sexes (gilts vs. barrows). The treatments were: 1) i.m. injection of GRA (two injections, 0.2 mg/kg BW at -1 and 3 d post-weaning), 2) two levels of in-water GRA (0.8 and 1.6 ppm), 3) in-feed GRA (2.5 and 5.0 ppm), 4) in-feed antibiotic (ANT, 100 ppm tylosin), and 5) control. Dexamethasone was used as GRA. Pigs had free access to in-feed or in-water GRA or ANT during the 1st-week post-weaning. Parameters of growth performance were measured weekly until the end of the nursery phase. A completely randomized design and repeated measurement ANOVA (PROC MIXED) in SAS were used for statistical analyses. In-water, in-feed, and i.m. GRA treatments resulted in growth performance outcomes comparable to that of in-feed ANT. Relative to CON pigs, both ANT and GRA improved ADG and G: F during the nursery phase (P &lt; 0.05). Among the delivery methods tested, 2.5 ppm of in-feed GRA resulted in superior ADG (GRA vs. CON; 0.29 vs. 0.20 kg/d) and G: F (0.63 vs. 0.40 ± 0.05), compared to the control group (P &lt; 0.02). Collectively, these results suggested that in-feed treatment is the best method for delivering GRA to newly weaned pigs.


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