scholarly journals Screening of Antibacterial Metabolites from Marine Soil, Kodiyampaiayam, Tamilnadu

2019 ◽  
Vol 8 (3S3) ◽  
pp. 571-575
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Drug Development ◽  
Marine Environment ◽  
Marine Sediment ◽  
Crude Extract ◽  
Pathogenic Bacteria ◽  
Soil Samples ◽  
Morphology Characteristic ◽  
New Antibiotics

The marine environment is a rich resource for isolating exploited microorganisms. In recent years, antibiotics have become important in this study of new antibiotics that show antiviral, anticoagulant and cardiac properties. Soil samples were collected from different area and isolated the bacterial colonies were separately streaking in agar plates (KP1, KP3, KP6, KP7 and KP9). The bacteria were done by morphology characteristic after that bacterial crude extract was taken separately. The crude extract was used in antibacterial activity against human pathogenic bacteria. There KP 7 and KP 9 havethe highest activity in B. subtilis and S. pyogenes rest of the samples also had activity comparing to these samples (KP 7 and KP 9) is highest zone inhibition developing. The marine sediment having so many microbes and secondary metabolites, therefore,the most useful drug development..

scholarly journals Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed Culture and Elicitation Conditions for the Production of Antimicrobial Compounds

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 673
Author(s):  
Kattia Núñez-Montero ◽  
Damián Quezada-Solís ◽  
Zeinab G. Khalil ◽  
Robert J. Capon ◽  
Fernando D. Andreote ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Culture Media ◽  
Genome Mining ◽  
Gene Clusters ◽  
Culture Conditions ◽  
Bioactive Metabolites ◽  
Bacterial Strains ◽  
Antarctic Bacteria ◽  
New Antibiotics

Concern about finding new antibiotics against drug-resistant pathogens is increasing every year. Antarctic bacteria have been proposed as an unexplored source of bioactive metabolites; however, most biosynthetic gene clusters (BGCs) producing secondary metabolites remain silent under common culture conditions. Our work aimed to characterize elicitation conditions for the production of antibacterial secondary metabolites from 34 Antarctic bacterial strains based on MS/MS metabolomics and genome mining approaches. Bacterial strains were cultivated under different nutrient and elicitation conditions, including the addition of lipopolysaccharide (LPS), sodium nitroprusside (SNP), and coculture. Metabolomes were obtained by HPLC-QTOF-MS/MS and analyzed through molecular networking. Antibacterial activity was determined, and seven strains were selected for genome sequencing and analysis. Biosynthesis pathways were activated by all the elicitation treatments, which varies among strains and dependents of culture media. Increased antibacterial activity was observed for a few strains and addition of LPS was related with inhibition of Gram-negative pathogens. Antibiotic BGCs were found for all selected strains and the expressions of putative actinomycin, carotenoids, and bacillibactin were characterized by comparison of genomic and metabolomic data. This work established the use of promising new elicitors for bioprospection of Antarctic bacteria and highlights the importance of new “-omics” comparative approaches for drug discovery.

scholarly journals Chemical Studies and Antibacterial Activity of the Root of Rumex abyssinicus

2020 ◽  
pp. 155-173
Author(s):  
Sisay Awoke ◽  
Chalachew Gedamu
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Chromatographic Separation ◽  
Pathogenic Bacteria ◽  
Endemic Plants ◽  
Chemical Studies ◽  
High Concentrations ◽  
Column Chromatographic ◽  
Antibacterial Evaluation

One of the Ethiopian endemic plants used traditionally as medicine for different diseases is Rumex abyssinicus. It is widely known as ‘Meqmeqo’ in Amharic. The aim of this study was to isolate and characterize compounds from the root of R. abyssinicus and to test their bioactivities of solvent fractions of the extract. The crude extract was extracted from dried powdered root of the plant with methanol. Then it was fractionated with n‑hexane and ethyl acetate with increasing polarity. The yellow powder obtained from ethyl acetate fractionation was applied to column chromatographic separation that led to the isolation of two compounds, diisobutyl phthalate and emodine. Finally, antibacterial evaluation of the ethyl acetate and methanol soluble portions were carried out against four pathogenic bacteria. Both methanol and ethyl acetate soluble portions inhibited all selected bacterial in better at high concentrations.

scholarly journals Extraction and Isolation of Compound from Roots of Kniphofia pumila, and Its Antibacterial Activities in Combination with Zinc Chloride

2020 ◽  
Vol 17 (7) ◽  
pp. 631-638
Author(s):  
Dele ABDISSA ◽  
Gezahegn FAYE ◽  
Melkamu FAYERA ◽  
Shiferaw DEMISSIE
Keyword(s):  
Antibacterial Activity ◽  
Crude Extract ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Spectroscopic Techniques ◽  
Bacterial Strains ◽  
Pure Compound ◽  
Standard Drug ◽  
Isolated Compound

Kniphofia pumila has been traditionally used for the treatment of different diseases in Oromiya regional state, western part of Ethiopia. Thus, the main objective of this study was to extract bioactive natural products from the roots of K. pumila and perform in vitro antibacterial activity tests against selected pathogens. Extraction of compound from the roots of K. pumila was carried out by maceration method at room temperature and its antibacterial activity was done using agar disc diffusion method. The crude extracts alone and along with ZnCl2 were tested against pathogenic bacteria Escherichia coli (ATCC 25722), Klebsiella pneumoniae subsp. pneumoniae (DSM 19613), Staphylococcus aureus (ATCC 25925) and Salmonella Typhimurium (ATCC 13311). Following its promising activity, the acetone crude extract that have shown better antibacterial activity was subjected to column chromatography for isolation of pure compound. Hence, one pure compound (GZ-1) was obtained from acetone crude extract. Meanwhile, the antibacterial activities of the isolated compound alone and in combination with ZnCl2 were also performed against all aforementioned bacterial strains. Then, isolated compound was characterized by using NMR spectroscopic techniques such as 1H-NMR, 13C-NMR and, 2D NMR, so as to establish its structure as 3’-acetyl-2’,6’-dihydroxy-4-methoxyphenyl-1,8-dihydroxy-3-methylanthraquinone, trivial name knipholone. Finally, GZ-1 was isolated from the roots of K. pumila and it was noted that the antibacterial activities of ZnCl2 in combination with crude extract as well as with isolated compound against E. coli (ATCC 25722), S. aureus (ATCC 25925) and S. Typhimurium (ATCC 13311) bacterial strains showed remarkable results which were greater than the antibacterial activity of the positive standard drug (gentamycin). To sum up, from the chromatographic isolation of roots acetone extract of K. pumila one pure compound (knipholone) was obtained. Its antibacterial activity was also evaluated in combination with ZnCl2 salt and alone.

scholarly journals New Bioactive Sesquiterpeniods From the Plant Endophytic Fungus Pestalotiopsis theae

2021 ◽  
Vol 12 ◽  
Author(s):  
Gaoran Liu ◽  
Ruiyun Huo ◽  
Yanan Zhai ◽  
Ling Liu
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Cell Lines ◽  
Crude Extract ◽  
Endophytic Fungus ◽  
Spectroscopic Data ◽  
Mcf7 Cell ◽  
Cytotoxic Effects ◽  
Electronic Circular Dichroism

Three new secondary metabolites pestalothenins A–C (1–3), including two new humulane-derived sesquiterpeniods (1 and 2) and one new caryophyllene-derived sesquiterpeniod (3), together with five known compounds (4–8) were isolated from the crude extract of the plant endophytic fungus Pestalotiopsis theae (N635). Their structures were elucidated by the extensive analyses of HRESIMS and NMR spectroscopic data. The absolute configurations of 1–3 were determined by comparison of experimental and calculated electronic circular dichroism (ECD) spectra. The cytotoxic effects of these compounds were evaluated in vitro. Compound 6 showed moderate cytotoxicity against T24 and MCF7 cell lines. In addition, compounds 1–8 were also evaluated for antibacterial activity.

scholarly journals Biological Activities and Chemical Characterization ofCordia verbenaceaDC. as Tool to Validate the Ethnobiological Usage

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Edinardo Fagner Ferreira Matias ◽  
Erivânia Ferreira Alves ◽  
Beatriz Sousa Santos ◽  
Celestina Elba Sobral de Souza ◽  
João Victor de Alencar Ferreira ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Synergistic Effect ◽  
Crude Extract ◽  
Hplc Analysis ◽  
Antibacterial Effect ◽  
Biological Activities ◽  
Chemical Characterization ◽  
Analgesic Agents

Knowledge of medicinal plants is often the only therapeutic resource of many communities and ethnic groups. “Erva-baleeira”,Cordia verbenaceaDC., is one of the species of plants currently exploited for the purpose of producing a phytotherapeutic product extracted from its leaves. In Brazil, its major distribution is in the region of the Atlantic Forest and similar vegetation. The crude extract is utilized in popular cultures in the form of hydroalcoholic, decoctions and infusions, mainly as antimicrobial, antiinflammatory and analgesic agents. The aim of the present study was to establish a chemical and comparative profile of the experimental antibacterial activity and resistance modifying activity with ethnopharmacological reports. Phytochemical prospecting and HPLC analysis of the extract and fractions were in agreement with the literature with regard to the presence of secondary metabolites (tannins and flavonoids). The extract and fraction tested did not show clinically relevant antibacterial activity, but a synergistic effect was observed when combined with antibiotic, potentiating the antibacterial effect of aminoglycosides. We conclude that tests of antibacterial activity and modulating the resistance presented in this work results confirm the ethnobotanical and ethnopharmacological information, serving as a parameter in the search for new alternatives for the treatment of diseases.

scholarly journals Antibacterial Activities of Actinomycete Isolates Collected from Soils of Rajshahi, Bangladesh

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Md. Ajijur Rahman ◽  
Mohammad Zahidul Islam ◽  
Md. Anwar Ul Islam
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Inhibitory Activity ◽  
Pure Culture ◽  
Agar Medium ◽  
Antibacterial Activities ◽  
Soil Samples ◽  
Indicator Bacteria ◽  
Gram Negative Bacteria ◽  
Gram Negative

This study was performed to isolate actinomycete colonies having antibacterial activity from soil samples collected from different places around Rajshahi, Bangladesh. Thirty actinomycete colonies were isolated in pure culture from five soil samples using Starch-casein-nitrate-agar medium. The isolates were grouped in five color series based on their aerial mycelia color and screened for their antibacterial activity against a range of test bacteria. Sixteen isolates (53.3%) were found to have moderate to high activity against four gram-positive and four gram-negative bacteria. Since many isolates showed inhibitory activity against indicator bacteria, it is suggestive that Bangladeshi soil could be an interesting source to explore for antibacterial secondary metabolites.

scholarly journals Antibacterial activity of Actinomycetes symbiont with seaweeds: a prosperous agent of animal antibacterial

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Bertoka Fajar S.P. Negara ◽  
. Riyanti ◽  
Bintang Marhaeni ◽  
Aradea B. Kusuma
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Pathogenic Bacteria ◽  
Pcr Amplification ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
E Coli ◽  
Amplification Method ◽  
The Relationship

Circulation of synthetic antibacterial chemicals which is used continuously can cause resistance to bacteria. Alternative synthetic antibiotics research then comes up as an urgent need. Recently, the discovery of new antibacterial agents is looking for Actinomycetes. Actinomycetes are one of the active marine bacteria which produce secondary metabolites that could be used as antibacterial. The objective of this study was to evaluate the antibacterial activities of Actinomycetes symbiont with Halimeda macroloba, Gelidiella acerosa, and Turbinaria ornata in Pangandaran beach, Indonesia. A total of 41 Actinomycetes were isolated from 3 species of seaweed. Rep PCR amplification method was used to characterize and identify the relationship between samples. Primer BOX A1R (5’-CTACGGCAAGGCGACGCTGACGCTGACG-3’), 27F (5'-AGTTTGATCMTGGCTCAG-3') and 1492R (5'-TACGGYTACCTTGTTACGACTT-3') was used in amplification 16s DNA. Six isolates from the representative cluster dendogram were selected for secondary metabolites extraction using ethyl acetate solvent, meanwhile agar diffusion method was used as Antibacterial activity assay using E. coli, Proteus sp., and Enterobacter sp. as the bacterial test. The results showed that the activity of secondary metabolite extracts of Actinomycetes symbiont with Halimeda macroloba presented the highest inhibition zone on 3 bacterial tests. In the future, it could be a potential antibacterial agent against animal pathogenic bacteria.

scholarly journals Antimicrobial Activity of Fungal Extract of The Aspergillus flavus from Hiri Island, North Maluku to Pathogenic Bacteria

2020 ◽  
Vol 23 (1) ◽  
pp. 127
Author(s):  
Sri Sedjati ◽  
Ambariyanto Ambariyanto ◽  
Agus Trianto ◽  
Endang Supriyantini ◽  
Ali Ridlo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Metabolites ◽  
Aspergillus Flavus ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Resistant Bacteria ◽  
Malt Extract ◽  
Antibacterial Compounds ◽  
New Antibiotics ◽  
Inhibition Zones

Antibacterial compounds from sponge association fungi are one of the alternatives to search for new antibiotics against resistant bacteria. This study aims to explore the secondary metabolites potential from sponge association fungi as MDR pathogens antibacterial and to cultivate these fungi using a variety of cultivation media. TE-BO-09.1. Isolate can inhibit 3 bacteria, K. pneumoniae, B subtilis, and S. aureus, but is not able to inhibit E. coli and P. aeruginosa. Cultivation using standard media of Malt Extract Agar (MEA) and media modified from fish broth (M1, M2) and cassava infusion (M3, M4) produce secondary metabolites with varying quantities and antimicrobial activity. The inhibition zone of the produced extract with 500 μg/disc concentration ranged from 7.14 to 10.32 mm. The strongest potential was shown by ethyl acetate extract from isolates cultured with M2 (9 days cultivation), of which is able to produce 10.32 mm inhibition zones against S. aureus and methanol extracts from isolates cultured with M4 (6 days cultivation), and able to produce 10.05 mm inhibition zones against K. pneumoniae. In conclusion, the fungus  Aspergillus flavus can be culture using fish broth added glucose and cassava infusion water added peptone media to potentially produce antibacterial compounds against MDR pathogens.

scholarly journals Antibacterial and Cytotoxic Activities of Crude Ethyl Acetate Extract of Streptomyces sp. FEAI-1 Isolated From Soil Samples of Rajshahi, Bangladesh

2018 ◽  
Vol 20 (2) ◽  
pp. 188-193
Author(s):  
Most Farida Khatun ◽  
Md Uzzal Haque ◽  
Md Anwar Ul Islam
Keyword(s):  
Ethyl Acetate ◽  
Crude Extract ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Pharmaceutical Journal ◽  
Salmonella Typhi ◽  
Soil Samples ◽  
Dilution Method ◽  
Cytotoxic Activities ◽  
Streptomyces Species

The present study was aimed to isolate and investigate actinomycetes having antimicrobial and cytotoxic activities from soil samples of Mirzapur, Rajshahi, Bangladesh. Total 27 bacteria were isolated and initial screening found that Streptomyces species have low to moderate antagonistic property against various pathogenic bacteria and among them EFAI-1was quite interesting. The antimicrobial activity of crude ethyl acetate extract obtained from EFAI-1was determined using broth-dilution method against Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Shigella sonnei and Salmonella typhi. The crude extract was almost equally active against both Gram-positive and Gramnegative bacteria. The highest zone of inhibition was found 21 mm at a concentration of 100 μg/disc against Salmonella typhi. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the crude extract against the test bacteria were in the range of 15.6-125 μg/ml and 62.5-250μg/ml, respectively. We found that the LC50 value of the crude extract was only 0.13 μg/ml against brine-shrimp nauplii indicating its potent cytotoxic nature. Our results indicate that this bacterium would be an excellent source of potent antibiotic and anticancer drugs.Bangladesh Pharmaceutical Journal 20(2): 188-193, 2017

scholarly journals Phytochemical Profile of Saribus rotundifolius (Anahaw) Crude Extract Found in the University of Eastern Philippines

2021 ◽  
pp. 250-253
Author(s):  
Flyndon Mark S. Dagalea ◽  
Karina Milagros C. Lim ◽  
Manuela Cecille G. Vicencio ◽  
Ma. Lourdes C. Alvarez ◽  
Maria Judy M. Somoray ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Drug Development ◽  
Crude Extract ◽  
Cardiac Glycoside ◽  
Ethanol Solution ◽  
Crude Extracts ◽  
Phytochemical Profile ◽  
Microbial Potential ◽  
The University

Phytochemical profiling focuses on the determination of the secondary metabolite present on a sample. Aims: In this research, a phytochemical profiling was done in Saribus rotundifolius (Anahaw) leaves and fruit crude extracts. Methodology: This study determines the secondary metabolites; alkaloid, tannin, flavonoid, cardiac glycoside, saponin, and terpenoid. The samples were cut into pieces and was submerged in an ethanol solution for the extract of crude extract and undergo a rotary evaporator for the separation of the ethanol solvent. Results: The finding showed that the anahaw leaves and fruit crude extract contains secondary metabolites present in both leaves and fruit crude extracts, that can be used in pharmaceutical and drug development, this includes an anti-microbial potential.

Sign in / Sign up

Export Citation Format

Share Document