scholarly journals Standardisasi Parameter Spesifik dan Non Spesifik Ekstrak Etanol Buah Asam Gelugur (GarciniaAatroviridis Griff.)

2019 ◽  
Vol 11 (02) ◽  
pp. 16-26
Author(s):  
Galih Samodra
Keyword(s):  
Body Weight ◽  
Traditional Medicine ◽  
Water Content ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Ash Content ◽  
Drying Rate ◽  
Reduce Body Weight ◽  
Specific Parameter

Acid gelugur fruit (Garcinia atroviridis) is one of the Garcinia families that has long been used as traditional medicine. Gelugur acid has been widely used as a cooking spice by Malay people, but it also has the ability to reduce cholesterol. Besides that, it has antioxidant properties and can reduce body weight and cholesterol. The purpose of this study was to establish specific parameter standards and non-specific parameters from the ethanol extract of acid gelugur fruit. The extract was obtained by maceration method using 70% ethanol with a rendament of 37.15%. Specific parameters include observations of organoleptic extracts of tamarind fruit showed, dark brown, distinctive smelling, and sour. Contains several sekendur metabolites such as flavonoids and saponins. The level of the compound that is soluble in water is 3.6%, while the content of the compound dissolved in ethanol is 3.9%. Total ash content of 2.99%. Non-specific parameters include acid insoluble ash content of 1.03%. The drying rate of geluguric acid simplicia was 7.20%. Water content of sour fruit gelugur extract was 13%.  

scholarly journals Pharmacognostic Study and Antioxidant Activity of Mundar (Garcinia forbesii King.) leaves from Banua Botanical Gardens of South Kalimantan

2020 ◽  
Vol 3 (4) ◽  
pp. 209-215
Author(s):  
Sutomo Sutomo ◽  
Dina Noor Kamali ◽  
Arnida Arnida ◽  
Normaidah Normaidah ◽  
Agung Sriyono
Keyword(s):  
Antioxidant Activity ◽  
Drying Shrinkage ◽  
Ethanol Extract ◽  
Ash Content ◽  
Water Soluble ◽  
Soluble Extract ◽  
Extract Content ◽  
Dpph Method ◽  
Specific Parameter ◽  
Parameter Test

Mundar (Garcinia forbesii King.) is a plant from South Kalimantan. This plant has chemical contents that have potential as medicine. The purpose of this study is to provide a pharmacognostic picture of a specific, nonspecific and determine the antioxidant activity of G. forbesii leaves. Specific parameters include organoleptic, microscopic testing, thin-layer chromatography profiles, and phytochemical screening. Nonspecific parameters include total ash content, acid insoluble ash content, drying loss, water-soluble extract content, and ethanol-soluble extract content. Antioxidant activity was determined by the DPPH method based on IC50 values. Specific parameter test results are green powder, characteristic odor, and sour taste. Microscopic tests showed stomata, upper epidermis, lower epidermis, cell walls, xylem, phloem, palisade tissue, spongy tissue, and cuticles. Garcinia forbesii leaves contain alkaloids, flavonoids, phenols, tannins, and steroids. The TLC profile showed good separation of polar eluents, and yellow spots appeared after spraying DPPH reagents. The non-specific parameter test is the total ash content of 6.52�0.1%; acid insoluble ash content 1.06�0.08%; drying shrinkage 6.43�0.38%; water-soluble extracts 34.3�0.3%; and ethanol-soluble extracts 23.47�0.35%. Ethanol extract of G. forbesii leaves has antioxidant activity with IC50 of 65.7 ppm. Pharmacognostic study fulfills the requirements, and G. forbesii leaves extract has strong antioxidant activity.

scholarly journals STANDARISASI NON SPESIFIK EKSTRAK ETANOL DAUN KECAPI (Sandoricum koetjape Merr.) DAN EKSTRAK ETANOL DAUN KELUWIH (Artocarpus communis)

2020 ◽  
Vol 16 (02) ◽  
pp. 105
Author(s):  
Maria Ulfah ◽  
Dhia Salsabilla ◽  
Evi Sukawati
Keyword(s):  
Heavy Metal ◽  
Water Content ◽  
Metal Contamination ◽  
Heavy Metal Contamination ◽  
Ethanol Extract ◽  
Ash Content ◽  
Contamination Level ◽  
Test Results ◽  
Standard Parameter ◽  
Sandoricum Koetjape

ABSTRACT The chemicals contained in an extract must be maintained its quality. The aim of this study is to prove both that the ethanol extract of kecapi (Sandoricum koetjape, Merr.) and keluwih (Artocarpus communis) leaves meet the non-specific parameters on the standardization of the extract. The ethanol extract of kecapi and keluwih leaves were extracted using the ultrasonic method. The extract was tested with non-specific parameters including water content, total ash content and metal contamination level. The test results are compared with standard parameter references. Ethanol extract of kecapi leaf have water content of 27.75% ± 1.708, total ash content of 4.856% ± 0.040 and levels of As metal contamination < 0.030 mg/Kg, Hg < 0.002 mg/Kg, Pb < 0.400 mg/Kg, while ethanol extract of keluwih leaf have water content of 23.206 ± 0.091%, total ash content of 9,678 ± 0.306% and levels of heavy metal contamination of As < 0.03 mg/Kg, Hg < 0.006 mg/Kg, Pb < 0.02 mg/Kg. The both extract meet the standard parameters of water content, total ash content and levels heavy metal contamination. Key words: kecapi leaves (Sandoricum koetjape, Merr.), keluwih leaves (Artocarpus communis), non-specific standardization

scholarly journals STANDARISASI NON SPESIFIK EKSTRAK ETANOL DAUN KECAPI (Sandoricum koetjape Merr.) DAN EKSTRAK ETANOL DAUN KELUWIH (Artocarpus communis)

2019 ◽  
Vol 16 (02) ◽  
pp. 20
Author(s):  
Maria Ulfah ◽  
Dhia Salsabilla ◽  
Evi Sukawati
Keyword(s):  
Heavy Metal ◽  
Water Content ◽  
Metal Contamination ◽  
Heavy Metal Contamination ◽  
Ethanol Extract ◽  
Ash Content ◽  
Contamination Level ◽  
Test Results ◽  
Standard Parameter ◽  
Sandoricum Koetjape

ABSTRACT The chemicals contained in an extract must be maintained its quality. The aim of this study is to prove both that the ethanol extract of kecapi (Sandoricum koetjape, Merr.) and keluwih (Artocarpus communis) leaves meet the non-specific parameters on the standardization of the extract. The ethanol extract of kecapi and keluwih leaves were extracted using the ultrasonic method. The extract was tested with non-specific parameters including water content, total ash content and metal contamination level. The test results are compared with standard parameter references. Ethanol extract of kecapi leaf have water content of 27.75% ± 1.708, total ash content of 4.856% ± 0.040 and levels of As metal contamination < 0.030 mg/Kg, Hg < 0.002 mg/Kg, Pb < 0.400 mg/Kg, while ethanol extract of keluwih leaf have water content of 23.206 ± 0.091%, total ash content of 9,678 ± 0.306% and levels of heavy metal contamination of As < 0.03 mg/Kg, Hg < 0.006 mg/Kg, Pb < 0.02 mg/Kg. The both extract meet the standard parameters of water content, total ash content and levels heavy metal contamination. Key words: kecapi leaves (Sandoricum koetjape, Merr.), keluwih leaves (Artocarpus communis), non-specific standardization

scholarly journals Pupuk Terhadap Kandungan Proksimat dan Mineral pada Suruhan Peperomia pellucida

2021 ◽  
Vol 23 (2) ◽  
pp. 60
Author(s):  
Yusrin Anton Puluhulawa ◽  
Novri Youla Kandowangko ◽  
Margaretha Solang
Keyword(s):  
Diabetes Mellitus ◽  
Content Analysis ◽  
Traditional Medicine ◽  
Protein Content ◽  
Water Content ◽  
Organic Fertilizer ◽  
Fat Content ◽  
Ash Content ◽  
Organic Fertilizers ◽  
Alternative Food

<p>Suruhan plant is one of the plants that has properties for treatment in various diseases such as diabetes mellitus, headache, fever, and rheumatism. In addition to traditional medicine, people usually use plants as an alternative food that is consumed by sautéing or consumed directly. The plant contains antioxidants, antimicrobials and anticancers. The leaves have flavonoids, alkaloids. Proximate content analysis is important to be used as data on the content of a foodstuff. One way to increase the content to be more useful is by cultivating using organic fertilizer. The purpose of this research is to find out the influence of organic fertilizers on the content of proximate, minerals on the plants. the method used was descriptive quantitative. Based on the results showed that on the content of proximate and minerals in the commission plants given organic fertilizer obtained water content of 26.60%, ash content of 16.80%, fat content of 2.58%, protein content of 13.86%, carbohydrates 40.16%, iron (Fe) 18.74 ppm, calcium (Ca) 255.92 ppm.</p>

scholarly journals Chemical Preliminary Evaluation of leaves, Peels, and Fleshs Fruit of Limau Kuit: Local Orange of South Kalimantan

2017 ◽  
Vol 11 (2) ◽  
pp. 71
Author(s):  
Azidi Irwan ◽  
Kamilia Mustikasari ◽  
Dahlena Ariyani
Keyword(s):  
Water Content ◽  
Fruit Juice ◽  
Ethanol Extract ◽  
Preliminary Test ◽  
Ash Content ◽  
Hexane Extract ◽  
Leaf Water Content ◽  
Preliminary Evaluation ◽  
Fruit Peel ◽  
Fruit Flesh

This research was conducted to know about fruit of limau kuit from South Kalimantan which includes weight, diemeter of fruit, volume of fruit juice, water content, and ash content of its flesh as well as water and ash content on leaves and fruit peel. Chemical preliminary examination includes measurement of pH of fruits juice and phytochemical screening test on leaves, fruit peel, and fruit flesh. The result is obtained with mean value as follows: weight 154,82 g, diameter offruit 68,45 mm, volume of juice water 44,60 ml, water content of fruit flesh 67,16%, water content of fruit peel 65,67%, leaf water content 65,88%, ash content 0,72%, fruit ash content 1,39%, and leaf ash content 3,32%. Measurement of pH of fruit juice without dilution; 100x; 1000x; and 10000x dilutions showing a value of 1.62; 2.08; 2.68; and 3.33, respectively. The results of the phytochemical examination on fresh samples of leaves, fruit peels, and fruit juices showed all samples giving positive (+) test results against alkaloids, saponins, steroids, triterpenoids (except negative (-) for fruit skin samples), tannins, and flavonoids (except (-) for the juice of the fruit). While the samples with the fresh-dried treatment extracted each using ethanol and n-hexane solvents showed (+) results for all sample extracts in both fresh-dried treatments, except (-) saponins in dried leaves (ethanol extract) and fruit peels fresh (n-hexane extract). Tannin test for fresh leaf (n-hexane) and flavonoid for fresh leaves (ethanol extract) and fresh fruit peel (ethanol extract) showed negative results.Keywords: Limau kuit, South Kalimantan, chemical preliminary test, phytochemical test, ethanol extract, n-hexane extract.

scholarly journals STANDARDISASI EKSTRAK PEGAGAN, CENTELLA ASIATICA SEBAGAI OBAT HERBAL TERSTANDAR HEPATOPROTEKTOR

2015 ◽  
Vol 17 (2) ◽  
pp. 185-193
Author(s):  
Puspa Dewi N Lotulung ◽  
Sri Handayani ◽  
Teni Ernawati ◽  
Tri Yuliani ◽  
Nina Artanti ◽  
...  
Keyword(s):  
Body Weight ◽  
Microbial Contamination ◽  
Raw Materials ◽  
Herbal Medicines ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Ash Content

Herbal medicinal products would be affected by the quality of raw materials. In turn, the quality of raw material will also be influenced by various factors such as soil conditions, cultivation, post-harvest processing, and the processing of raw materials into crude drug or extract. Therefore, in order to make good herbal medicines, it is necessary to make standardization of herbal extracts that produced herbal medicines that have the same quality and functions of effectiveness in each process. From preliminary studies that have been done, Centella asiatica is one of the potential plants as a source of hepatoprotective compounds. Test in vivo and in vitro against Centella asiatica extracts have shown very good results. Ethyl acetate extract with 17.5 mg/kg of doses body weight and butanol 228.8 mg/kgof doses body weight has been applied for in vivo test using mice induced by CCl4; theydemonstrated hepatoprotective effects. Ethyl acetate extracts were able to reduce levels of the enzyme alanine aminotransferase (ALT) and aspartate aminotransferase (AST) by 56 % and 44 % respectively while butanol extract can reduce the enzymes AST levels by 3%. Standardizationof Centella asiatica extract performed in this study was the characterization of the extract in the form of non-specific and specific parameters corresponding to the reference of PPOMN (Ministry of health Republic of Indonesia, 2000) such as levels of drying shrinkage, ash content, total plate count microbial contamination, levels of water-soluble compounds, levels of compounds that are soluble in ethanol, phytochemical test, total phenolic content, total flavonoid content and the determination of Pb and Cd weight.The results showed that non-specific parameters for the ethanol extract of Centella asiatica were requirements based on Herbal Pharmacopoeia in 2008 which includes parameters such as determination of shrinkage on drying ≤ 10%, ash content ≤ 16.6% and negative microbial contamination. Specific parameters for the ethanol extract of Centella asiatica have met the requirements of Herbal pharmacopeia in 2008.Keywords: Centellaasiatica, hepatoprotective, standardized herbal medicine, specificparameters, and non-specific parameters

scholarly journals Studi Farmakognostik dan Uji Parameter Nonspesifik Ekstrak Metanol Kulit Batang Kasturi (Mangifera casturi Kosterm.)

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Sutomo Sutomo ◽  
Nadya Agustina ◽  
Arnida Arnida ◽  
Fadilaturrahmah Fadilaturrahmah
Keyword(s):  
Water Content ◽  
Methanol Extract ◽  
Sweet Taste ◽  
Ash Content ◽  
Fruit Skin ◽  
Specific Parameter ◽  
Chemical Content ◽  
Orange Colour ◽  
Bacteria And Fungi ◽  
Total Bacteria

ABSTRAK Kasturi (Mangifera casturi Kosterm.) merupakan salah satu tumbuhan endemik Kalimantan Selatan. Kulit batang M. casturi mengandung beberapa golongan senyawa yang berpotensi sebagai obat. Penelitian ini bertujuan untuk memberikan data hasil analisis farmakognostik, batas-batas maksimal kandungan senyawa tertentu dan profil kromatogram kandungan kimia ekstrak metanol kulit batang M. casturi. Analisis farmakognostik terhadap tumbuhan meliputi morfologi, anatomi, dan identifikasi kandungan kimia. Batasan maksimal kandungan senyawa tertentu berdasarkan parameter non spesifik meliputi kadar air, kadar abu total, kadar abu tidak larut asam, total bakteri dan kapang, dan kadar Pb dalam ekstrak. Karakteristik tumbuhan M. casturi yaitu memiliki batang berwarna coklat tua dengan permukaan kasar dan bergetah, daun berwarna hijau, berbentuk lancet, kulit buah matang berwarna coklat keunguan, daging buah berwarna kuning terang hingga jingga, berbau khas, berasa manis agak asam dan banyak mengandung serabut. M. casturi mengandung senyawa alkaloid, flavonoid, terpenoid, fenol dan saponin yang dibuktikan berdasarkan uji identifikasi kimia dan analisis secara KLT. Pengujian parameter non spesifik ekstrak metanol kulit batang M. casturi secara berturut-turut yaitu, kadar air 12,5±0,7%; kadar abu total 1,0±0,5%; kadar abu tidak larut asam 0,67±0,28%; tidak terdeteksi adanya pertumbuhan bakteri pada ekstrak kulit batang M. casturi; sedangkan total kapang yaitu 250 koloni/g; dan kadar Pb yaitu 3 mg/kg. Kata Kunci: Kasturi, analisis, farmakognostik, Mangifera casturi, parameter non spesifik ABSTRACT Kasturi (Mangefera casturi Kosterm.) is one of the endemic plants of South Kalimantan. M. casturi bark contain several group of compounds potential to the treatment. This research aims to provide data analysis results pharmacognostic, maximum limits of certain compounds and profile chromatogram of chemical content of methanol extract from M. casturi bark. Pharmacognostic analysis of the plant include morphology, anatomy, and the identification of compounds. The maximum limits of certain coumpounds by non-specific parameters include water content, total ash, ash content insoluble in acid, total bacteria and fungi, and Pb content in the extract. M. casturi characteristic is having dark-brown stems with roughly surface and sticky, green leaves, lancet-shapes, fruit skin has a purplish brown colour, flesh of fruit has a bright yellow till orange colour, distinctive smell, slightly sour sweet taste, and contains lots of fiber. M. casturi bark contains alkaloids, flavonoids, terpenoids, phenols, and saponins that has been proved by chemical identification test and analysis of TLC. Non-specific parameter testing about methanol extract of M. casturi bark consecutively given, water content 12,5±0,7%; total ash 1,0±0,5%; ash content insoluble in acid 0,67±0,28%; undetected any bacterial contamination in the extract of M. casturi bark; while the total mold contamination is 250 colonies/g; and Pb contents is 3 mg/kg. Key words: Kasturi, pharmacognostic analysis, Mangifera casturi, non-specific parameter

scholarly journals Uji Efek Kombinasi Amoksisilin Dengan Ekstrak Etanol Daun Sirih (Piper Betle L) Terhadap Pertumbuhan Bakteri Escherichia Coli Dan Staphylococcus Aureus

2018 ◽  
Vol 1 (1) ◽  
pp. 313-319
Author(s):  
Panal Sitorus
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Water Content ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Ash Content ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Extract Content ◽  
And Staphylococcus Aureus

Konsep baru untuk pengobatan infeksi bakteri resisten adalah dengan kombinasi antibiotika dengan ekstrak herbal. Ekstrak daun sirih memiliki aktivitas anti bakteri terhadap Escherichia coli dan Staphylococcus aureus kerena daun sirih mengandung metabolit sekunder. Kombinasi ekstrak daun sirih dengan amoksisilin, perlu diuji untuk mengetahui efek antibaktreri masing-masing dibandingkan dengan kombinasinya. Mengetahui karakteristik dan skrining fitokimia simplisia dan ekstrak daun sirih serta uji aktivitas anti bakteri kombinasi amoksisilin dengan ekstrak daun sirih terhadap Escherichia coli dan Staphylococcus aureus. Penelitian ini meliputi ekstraksi serbuk simplisia dengan maserasi, Karakterisasi simplisia untuk mengetahui kadar air, kadar sari larut dalam air, kadar sari larut dalam etanol, kadar abu total dan kadar abu tidak larur asam, serta skrining fitokimia simplisia dan ekstrak daun sirih serta uji aktivitas anti bakteri kombinasi amoksisilin dengan ekstrak daun sirih terhadap Escherichia coli dan Staphylococcus aureus dengan metode difusi agar menggunakan cakram kertas. Hasil penelitian menunjukkan bahwa serbuk simplisia daun sirih memiliki kadar air 3,66%; , kadar sari larut dalam air 22,54%, kadar sari larut dalam etanol 14,65%, kadar abu total9,25% dan kadar abu tidak larur asam 1,03%. Komponen kimia pada simplisia dan ekstrak etanol serbuk daun sirih adalah flavonoid, tannin, saponin dan steroid. Ekstrak etanol serbuk daun sirih menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus masing-masing pada KHM 8 mg/ml dan 9 mg/ml. Amoksisilin menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus masing-masing pada KHM 5 g/ml dan 10 g/ml dengan diameter zona hambat 6,83mm dan 7,05 mm. Diameter zona hambat terhadap Escherichia coli dan Staphylococcus aureus meningkat ketika bakteri uji dipaparkan dengan kombinasi ekstrak etanol daun sirih dengan amoksisilin. Dari hasil penelitian menunjukkan bahwa kombinasi Amoksisilin dengan Ekstrak etanol daun sirih menunjukkan efek yang sinergis. A new concept to treat resistant bacterial infection is using antibiotic combination with herbal extract. Betel leaves extract has an anti-bacteria activity towards Escherichia coli and Staphylococcus aureus since it contains secondary metabolite. Betel leavesextract combination with amoxicillin is needed to be tested to find evaluatetheiranti-bacteria effects and compare with its combination. By finding the characteristics and phytochemical screening simplicia and the betel leaves extract and also the anti-bacteria activity of amoxicillin combination with betel leaves to Escherichia coli and Staphylococcus aureus. This research covered extraction of simplicia powder using macerationmethod. The simplicia characteristics were conductedto determinethe water content, dissolved extract content in water, dissolved extract content in ethanol, total ash, and the acidic undissolved ash, and also the phytochemical screening of simplicia and the betel leaves extract and also the anti-bacteria activity of amoxicillin combination with betel leaves to Escherichia coli and Staphylococcus aureus using diffusion method using paper disc method. The result indicated that the betel leaves powder simplicia had 3.66% of water content, 22.54% of dissolved content extract in water, 14.65% of dissolved content extract in ethanol, 9.25% of ash content, and 1.03% of acidic undissolved ash content. The chemical components in simpliciaand ethanol extract of betel leaves were flavonoid, tannin, saponin, and steroid. The ethanol extract of betel leaves inhibited the growth of Escherichia coli and Staphylococcus aureus bacteria with MIC of 8 mg/ml and 9 mg/ml. Amoxicillin inhibited the growth of Escherichia coli and Staphylococcus aureus bacteria with MIC of5 g/ml and 10 g/ml with diameter of inhibition zone of 6.83 mm and 7.05 mm, respectively. The diameter of inhibition zone towards Escherichia coli and Staphylococcus aureus increased when the bacteria exposed to the combination of betel leaves ethanol extract and Amoxicillin. The research indicated that Amoxicillin combination with betel leaves ethanol extract showed synergistic effect.

UJI IN VITRO EKSTRAK AIR DAN ETANOL DARI BUAH ASAM GELUGUR, RIMPANG LENGKUAS, DAN KENCUR SEBAGAI INHIBITOR AKTIVITAS LIPASE PANKREAS

2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Dyah Iswantini ◽  
Latifah K. Darusman ◽  
Ana Fitriyani
Keyword(s):  
Body Weight ◽  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Water Extract ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Reduce Body Weight ◽  
Ethanol Extracts

Asam gelugur fruits of Garcinia often used to reduce body weight. Lengkuas and kencur are traditional herbal that potential for antiobesity because they could reduce the level of phospholipids, triglycerides, and cholesterol. The aim of the research was to evaluate the potencies of these herbal as antiobesity by measurement of their water and ethanol extracts capabilities as in vitro inhibitor of pancreatic lipase activity. The water and ethanol extracts of asam gelugur fruits contained saponins and alkaliods, respectively. The water extract of lengkuas rhizomes contained alkaloids, flavonoids, saponins, and quinones, while the ethanol extract contained alkaloids, flavonoids, saponins, and steroids. The water extract of kencur rhizomes contained saponins and quinones, while the ethanolextract contained alkaloids, flavonoids, steroids, and quinones. The highest inhibitory effect of all extracts was obtained from the ethanol extract of asam gelugur fruits with value of 86.3% at 150 ppm. The highest inhibitory effect of lengkuas extracts was from the ethanol extract at 200 ppm (56.2%). The highestinhibitory effect of kencur was showed by the ethanol extract with the value 37.6% at 300 ppm. These values were higher than the inhibitory effect of the positive control (Xenical®) at 100 ppm (10.6%).

scholarly journals Inhibisi Lipase Pankreas secara In Vitro oleh Ekstrak Air dan Etanol Daun Asam Jawa (Tamarindus indica) dan Rimpang Kunci Pepet (Kaempferiae rotundae)

2012 ◽  
Vol 13 (2) ◽  
pp. 146
Author(s):  
Dyah Iswantini Pradono ◽  
Latifah Kosim Darusman ◽  
Ai Susanti
Keyword(s):  
Body Weight ◽  
Pancreatic Lipase ◽  
Water Extract ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Sesame Oil ◽  
Tamarindus Indica ◽  
Reduce Body Weight ◽  
Ethanol Extracts

Tamarind and kunci pepet has been used traditionally as herbal medicine to reduce body weight or used asantiobesity. But the mechanism in vitro of this herbal in reducing body weight has not been known yet. Theobjective of this research is to evaluate these herbal as antiobesity by their water and ethanol extracts capabilityin inhibiting pancreatic lipase activity in vitro at pH 8, incubation time 45 minutes, and temperature 400C. Pancreaticlipase used in this research was human pancreatic lipase with concentration of 1.4 × 10-5 μg/μl and the substratewas sesame oil with concentration of 16.2 μg/μl. The water and ethanol extracts of tamarind leaves containedalkaloids, flavonoids, saponins, steroids, and tannins. Water extract of kunci pepet contained alkaloids, flavonoids,saponins, and tannins, while ethanol extract contained alkaloids, flavonoids, saponins, and triterpenoids. Theresults of tamarind leaves extracts showed that ethanol extract in concentration of 150 ppm had the highestinhibitory effect, with the value of 49.0%. Water extract of kunci pepet at concentration of 200 ppm had the highestinhibition, with the value of 65.1%. These values were higher than inhibitory effect of Xenical® 100 ppm as thepositive control, with the inhibition value of 10.6%.

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