scholarly journals Embryotoxic and teratogenic effects of ‘Vitosept’ on white rats

2019 ◽  
Vol 5 (4) ◽  
pp. 22-26
Author(s):  
M. P. Soltys ◽  
H. V. Rudyk ◽  
V. M. Gunchak ◽  
B. V. Gutyi
Keyword(s):  
Sodium Hypochlorite ◽  
High Purity ◽  
Female Rats ◽  
Control Group ◽  
Sodium Hypochlorite Solution ◽  
Teratogenic Effects ◽  
Pregnant Females ◽  
White Rats ◽  
Group I ◽  
Significant Difference

The effect of ‘Vitosept’ drug, based on sodium hypochlorite solution with high purity, obtained in a specially developed membraneless flow electrolyzer, on the embryotoxic and teratogenic properties in rats was investigated. To determine the embryotoxic effect of ‘Vitosept’ on the development of white rats offspring of the 1st generation, control and three experimental groups (G1, G2, G3) were formed from pregnant females. The females of the control group with a blunt probe were injected daily for 30 days with 5 ml of isotonic sodium chloride solution, and experimental ones with 5 ml of ‘Vitosept’ drug with different concentration of high purity sodium hypochlorite: Group I (G1) — 50 mg/l; Group II (G2) — 100 mg/l; Group III (G3) — 500 mg/l. The animals were observed. During the observation the condition and behavior of the females, the dynamics of body weight change, duration of pregnancy, and the course of birth were monitored. The results of the experiment were recorded after the slaughter of pregnant females (20th day of pregnancy) and in the postnatal period of development of the offspring. Studies have shown that the use of different concentrations of the drug ‘Vitosept’ in rats for 30 days before and during pregnancy has no embryotoxic and teratogenic effects. According to the indicators of the total, pre- and postimplantation lethality of embryos, there were no reliable changes in the structure and morphometry of internal organs and tissues in 20-day-old fetuses, and their development corresponded to the terms of pregnancy. There was no significant difference between the fertility of female rats in the test and control groups. The average number of fetuses per female was within 9 animals. The rats obtained from the females of the experimental groups were viable and did not lag behind in growth and development compared with the control animals, which generally characterizes the studied drug ‘Vitosept’ as non-toxic, lacking embryotoxic and teratogenic action

scholarly journals BLACK AND RED ONCOM EXTRACT SUPPLEMENTATION LENGTHEN ESTROUS CYCLE AND THICKEN ENDOMETRIUM OF WHITE RATS

2020 ◽  
Vol 21 (4) ◽  
pp. 558-564
Author(s):  
Hanifah Alshofa Nurul Aini ◽  
Desak Nyoman Dewi Indira Laksmi ◽  
Ni Luh Eka Setiasih ◽  
Steven Dwi Purbantoro
Keyword(s):  
Estrous Cycle ◽  
Feeding Tube ◽  
Vaginal Swab ◽  
Female Rats ◽  
Control Group ◽  
Antral Follicles ◽  
White Rats ◽  
Fertile Female ◽  
Group I ◽  
Significant Difference

hytoestrogens are active compounds, derived from plants, which have a similar structure and function as estrogen. Phytoestrogens are commonly found in legumes. Oncom, which is assumed containing phytoestrogens, is one of the most famous legumes food from Indonesia and widely consumed daily in West Java. This study was aimed to determine the effect of oncom extract on estrous cycle, endometrium thickness, and the number of antral follicles in productive age rats (Rattus novergicus). This experimental study was using 21 three-to-four-month-old fertile female rats and divided into three groups. Group I (K) was considered as a control group without any treatment. Group II and III were treatment groups which were given black (H) and red (M) oncom extracts 0.005 g/g body weight, respectively, orally with a feeding tube for 14 days. The length of the estrous cycle was measured by performing vaginal swab with interval 12 hours after first treatment was given and during the treatment. Endometrium thickness and the number of antral follicles were measured by collecting the organs uterus and ovary for histological purpose with paraffin method after rats were euthanized post-treatment oncom extracts for 14 days. Data were analyzed by ANOVA and continued with LSD test. The total length of estrous cycle of control group, black oncom extract group, and red oncom extract group was 107,43±3,16 hours, 141,43±15,36 hours, and 161,14±17,10 hours, respectively. The mean of endometrium thickness of control group, black oncom extract group, and red oncom extract group was 346,945±65,88 ?m, 485,740±86,69 ?m, and 533,904±78,93 ?m, respectively. The number of antral follicles of control group, black oncom extract group, and red oncom extract group was 6,00±1,54, 8,43±2,99, and 9,14±2,72, respectively. Results showed that black and red oncom extracts had a significant effect on the length of estrous cycle and endometrium thickness in rats, yet there is no significant difference in the number of antral follicles. In summary, black and red oncom extracts had effects on the length of estrous cycle and endometrium thickness, yet there was no effect on the number of antral follicles.

scholarly journals Effect of Application Time of Two Different Disinfectants on the Surface Roughness of an Elastomeric Impression Material

2020 ◽  
Author(s):  
Tahir Karaman ◽  
Faruk Oztekin ◽  
Samet Tekin
Keyword(s):  
Surface Roughness ◽  
Sodium Hypochlorite ◽  
Quaternary Ammonium ◽  
Rank Test ◽  
Control Group ◽  
P Value ◽  
Sodium Hypochlorite Solution ◽  
Application Time ◽  
Impression Material ◽  
Significant Difference

Introduction: Pathogens, such as cytomegalovirus, hepatitis B virus, hepatitis C virus, herpes simplex virus types 1 and 2, and human immunodeficiency virus are transmitted, threatening the health of dental laboratory workers, especially as a result of saliva and blood contact of patients. To prevent cross-infection, impression materials should be disinfected at the end of the impression process in the mouth. Aim: To study the effect of application time of sodium hypochlorite and quaternary ammonium-based disinfectant solution on the surface roughness of an elastomeric impression material. Materials and Methods: In this in-vitro study done during March 2020, 10 disc-shaped samples were used in each group, with a total of 110 samples obtained from a light body elastomeric impression material with dimensions of 15×3 mm. The samples were kept in a sodium hypochlorite solution (Wizard; Rehber Kimya, Istanbul, Turkey) at concentrations of 1% and 5% for 1, 5, 10, and 30 minutes and in a quaternary ammonium-based disinfectant (Zeta 7 Solution, Zhermack SpA, Italy) for 10 and 30 minutes. Surface roughness measurements were taken with a profilometer device. The data obtained were statistically analysed by Kolmogorov-Smirnov test and Wilcoxon signed rank test for dependent/paired groups for the continuous data. The significance level was set to α=0.05. Results: A statistically significant difference was found between the control group and the 1% sodium hypochlorite (30 min p-value 0.037), and 5% sodium hypochlorite (30 min p-value 0.017). The statistical evaluation of the surface roughness of the samples with different concentrations of sodium hypochlorite and the same times was done and found statistically significant at 30 mins, p-value 0.021. Conclusion: The prolonged application of the sodium hypochlorite disinfectant at 1% and 5% concentrations caused a significant increase in the light body elastomeric impression material’s surface roughness

scholarly journals IMBIBITION EFFECT OF IMMERSION HYDROCOLLOID IRREVERSIBLE ALGINATE IN SODIUM HYPOCHLORITE SOLUTIO

2015 ◽  
Vol 3 (2) ◽  
pp. 59-67
Author(s):  
Siti Coryniken ◽  
Didin Kustantiningtyastuti ◽  
Afriwardi Afriwardi
Keyword(s):  
Sodium Hypochlorite ◽  
Repeated Measure ◽  
Control Group ◽  
Sodium Hypochlorite Solution ◽  
Control Group Design ◽  
Impression Material ◽  
Hypochlorite Solution ◽  
Significant Difference ◽  
Weight Calculation ◽  
Disinfectant Solution

materials have character that absorb water it is imbibition that can affect the dimensional stability when immersed in disinfectant . The purpose of this study is to see the effect of imbibition at alginate impression material immersed in a disinfectant solution of Sodium Hypochlorite .The method used was experimental with pretest and posttest with control group design . The mold were diameter of 28 mm and height 18 mm .the impression were immersed in sodium hypochlorite 0.5 % and 1 % for 3 minutes , 5 minutes and 10 minutes . Imbibition weight calculation is done by using digital scales.The results showed that the presence of imbibition on impression material alginate that immersed in Sodium Hypochlorite solution for 3 minutes , 5 minutes and 10 minutes . Statistical Test Two Way Repeated Measure ANOVA showed a significant difference ( p< 0.05 ) in minutes imbibition , while the concentration of the solution had a significant difference ( p > 0.05 ) .The conclusion of this study is the alginate impression material has the effect of imbibition to immersion Sodium Hypochlorite solution. Keywords : hydrocolloid Alginate , Sodium Hypochlorite , imbibition effects , immersio

scholarly journals EFEKTIVITAS EKSTRAK ETANOL DAUN EFEKTIVITAS EKSTRAK ETANOL DAUN SIDAGURI (Sida rhombifolia L.) TERHADAP PENURUNAN KADAR ASAM URAT PADA TIKUS JANTAN GALUR WISTAR HIPERURISEMIA

2020 ◽  
Vol 3 (1) ◽  
pp. 29-37
Author(s):  
Bunga Rimta Barus
Keyword(s):  
Body Weight ◽  
Uric Acid ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Weight Group ◽  
White Rats ◽  
Group I ◽  
Significant Difference ◽  
Sida Rhombifolia

Sidaguri leaves (Sida rhombifolia L.) Have been shown to contain flavonoid compounds, where they are efficacious as hyperuricemia. This study aims to determine effectiveness ethanol extract of sidaguri leaves as antihyperuricemia in white rats. The study was conducted using the method of potassium oxanate induction and measured using glucometers. 15 animals were divided into 5 groups, namely negative control, positive control, group I combination of ethanol extract of sidaguri leaves 50 mg / kg body weight, group II ethanol extract of sidaguri leaves 100 mg / kg body weight, group III ethanol extract of sidaguri leaves 200 mg / kg body weight for 5 hours. Examination of antihyperuricemia effects is done by comparing the uric acid levels value of the rats before and after treatment. The results of this study indicate that the three dose ethanol extract of sidaguri leaves provide an antihyperuricemia effect and based on one-way Anova, Tuckey and LSD data analysis, shows that the dose of 50 mg / kg body weight is effective as antihyperuricemia and does not have a significant difference (p> 0.05). The conclusion of this study is that the dose is the most effective in decreases uric acid levels in white rats, which is a dose of 200 mg / kg body weight because it mostly decreased uric acid levels.

scholarly journals PERBEDAAN STABILITAS DIMENSI ANTARA CETAKAN ALGINAT YANG DISEMPROT DENGAN LARUTAN NATRIUM HIPOKLORIT 0,5% DAN DETTOL 5%

2017 ◽  
Vol 5 (1) ◽  
pp. 59-70
Author(s):  
Wira Putri Winata ◽  
Kuswardani Susari Putri ◽  
Febrian Febrian
Keyword(s):  
Sodium Hypochlorite ◽  
Dimensional Stability ◽  
Control Group ◽  
Sodium Hypochlorite Solution ◽  
Control Group Design ◽  
Hypochlorite Solution ◽  
Impression Materials ◽  
Significant Difference ◽  
Post Test ◽  
The Difference

The impression materials that most commonly used in dentistry was alginate. Factors that must be considered was the controlled of infection transmission from alginate, it was important to do disinfection using a disinfectant solution. Alginate had imbibition properties, so that disinfection by spraying technique allowed the expansion of alginate affecting the dimensional stability of impressions. The purpose of this research was to determine the difference between the dimensional stability of alginate impression were sprayed with a solution of sodium hypochlorite 0,5% and Dettol® 5%.  The method used was an experimental laboratory with post test only control group design. 27 samples were divided into 3 groups: spraying with 0.5% sodium hypochlorite solution, spraying with 5% Dettol® solution, and spraying with aquades as a controlled. Alginate impressions were sprayed with 0,5% sodium hypochlorite solution, Dettol® 5%, and aquades then filled with gypsum, and then measured dimensional stability by using micrometer screw. Data were analyzed with the Kruskal-Wallis test.  The results showed that differences between the dimensional stability of alginate impressions were sprayed with a solution of sodium hypochlorite 0.5% and 5% Dettol® was not significant (p> 0,05).  The conclusion of this research was there was no significant difference between the alginate impressions were sprayed with a solution of sodium hypochlorite 0,5% and Dettol® 5% to dimensional stability. Keywords : dimensional stability, alginate, sprayed, sodium hypochlorite, Dettol®

scholarly journals Comparison of Various Cold Sterilization Techniques on Routinely used Carbide Burs and Diamond Points

2017 ◽  
Vol 7 (3) ◽  
pp. 97-102
Author(s):  
Suganthi Ranganathan ◽  
Chaitra Koppal Renukanath
Keyword(s):  
Hydrogen Peroxide ◽  
Sodium Hypochlorite ◽  
Culture Media ◽  
Brain Heart Infusion ◽  
Control Group ◽  
Microbial Count ◽  
Group I ◽  
Significant Difference ◽  
Before And After ◽  
Post Hoc

ABSTRACT Aim The aim of this study is to compare the various cold sterilization techniques for sterilizing dental burs and diamond points by assessing the microbial growth in culture media on them before and after sterilization. Materials and methods The following four disinfectants were used: 2% glutaraldehyde, 5.2% sodium hypochlorite, hydrogen peroxide, and 70% ethanol. There were two main groups taken as group I-carbide burs and group II-diamond burs. A total of 48 samples were collected, 12 samples per disinfectant. Each sample has two burs collected from same patient. One acts as control group which means only microbial count was deter- mined without disinfection. The other one would be determined as microbial count before and after disinfection. The microbial assessment was done using brain heart infusion (BHI) broth and counted by plating in chocolate blood agar and MacConkey agar. Results The obtained results were statistically analyzed by one-way analysis of variance (ANOVA) test and post hoc test. The results revealed that there was statistically significant difference between sodium hypochlorite and surgical spirit, sodium hypochlo- rite and hydrogen peroxide. There was no statistically significant difference between sodium hypochlorite and glutaraldehyde. Conclusion The results of this study revealed that sodium hypochlorite and glutaraldehyde are most effective than hydro- gen peroxide, and surgical spirit is the least effective disinfectant. However, there was clinical significance in level of disinfection of all four disinfectants. How to cite this article Ranganathan S, Manvi S, Gopalakrishna S, Renukanath CK. Comparison of Various Cold Sterilization Tech- niques on Routinely used Carbide Burs and Diamond Points. Int J Prosthodont Restor Dent 2017;7(3):97-102.

Comparison of Repeated Chemical and Microwave Disinfection on Dimensional Accuracy of Gypsum Casts

2021 ◽  
Vol 30 (04) ◽  
pp. 235-242
Author(s):  
Mariya Khalid ◽  
◽  
Mohammad Ali Chughtai ◽  
Sohrab Shaheed ◽  
Syed Nasir Shah
Keyword(s):  
Sample Size ◽  
Sodium Hypochlorite ◽  
Dimensional Accuracy ◽  
Dimensional Change ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Group A ◽  
Group B

OBJECTIVE: The aim of this experimental study is to compare the dimensional accuracy of gypsum casts after repeated disinfection in microwave at 900 Watts, 2450 MHz (5 minutes) and immersion in 0.5% Sodium hypochlorite (10 minutes). Disinfecting casts is recommended to prevent cross infection but may cause dimensional changes. During fabrication of prosthesis, a cast may get contaminated several times so there is a need of repeated disinfection. METHODOLOGY: Sample size was 33 (11 in each group), calculated through WHO software for sample size determination by using standard deviation of 0.16 at 95% confidence interval and 80% power of study. Impressions in irreversible hydrocolloid were recorded of an acrylic cast fabricated for this study. The impressions were poured with die stone and were randomly divided into 3 groups; Group I: Microwave disinfection, Group II: Immersion disinfection in 0.5% Sodium hypochlorite, Group III: Control group. For Groups I and II, each cast was disinfected 7 times with 5 minutes interval between two disinfection cycles, after every cycle anteroposterior and mediolateral measurements were recorded using digital Vernier caliper (accuracy upto 0.01 mm). For group III, casts were rinsed with distilled water, dried in open air within temperature range of 28+/-2OC for 10 mins followed by anteroposterior and mediolateral measurements. This procedure was repeated seven times for each cast. RESULTS: Anteroposterior and Mediolateral differences of dimensional change between and within the Group A, B and C was calculated by One Way ANOVA. Inter/intra examiner reliability was taken into consideration at the time of study. Mean dimensional change in the casts were insignificant through six disinfecting cycles. However, in the seventh cycle, a significant difference (p=0.003) was observed in the anteroposterior dimension (0.03% dimensional change for Group A and 1.26 % for Group B whereas, in mediolateral dimension, dimensional change was 0.35% for Group A and 0.59% for Group B (p=0.004). Dimensional change of >0.5% was considered as the cutoff value for casts to be considered as dimensionally accurate. Casts disinfected through immersion disinfection did not produce dimensionally inaccurate casts in anteroposterior dimension after third cycle and in seventh cycle in mediolateral dimension. However, result is significant only in seventh cycle. Microwave disinfection produced dimensionally accurate casts throughout all cycles. CONCLUSION: Microwave disinfected casts remained dimensionally stable compared to immersion disinfection. KEYWORDS: Disinfection, microwave, immersion, dimensional stability, gypsum casts

scholarly journals In vitro study of the effect of Er: YAG laser irradiation on the apical sealing of different root canal sealers

2006 ◽  
Vol 14 (4) ◽  
pp. 260-263 ◽  
Author(s):  
Fernanda Vieira Medina ◽  
Manoel Damião Souza-Neto ◽  
Jacy Ribeiro Carvalho-Junior ◽  
Heid Sueli Leme dos Santos ◽  
Melissa Albanese Mezzena ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Er:Yag Laser ◽  
Sodium Hypochlorite Solution ◽  
Laser Application ◽  
Hypochlorite Solution ◽  
Root Canals ◽  
Group I ◽  
Significant Difference

OBJECTIVES: The aim of this study was to determine the effect of Er:YAG laser irradiation used to clean dentinal walls on the apical sealing of root canals filled with different types of sealers. Background Data: Laser application to the dentinal walls removed debris, rendering the root canals free of smear layers and leaving the dentinal canaliculi open. METHODS: Sixty-four maxillary canines obtained from laboratory files were instrumented with K-files (Dentsply, Maillefer, Ballaigues, Switzerland) using the crown-down technique, and irrigated with a 0.5% sodium hypochlorite solution. The specimens were divided into two groups of 32 teeth each. In group I, the teeth were instrumented and irrigated with sodium hypochlorite solution, and divided into four subgroups to be sealed with the different materials (Endofill, N-Rickert, Sealapex and Sealer 26). In group II, the root canals were subjected to Er:YAG laser irradiation (200 mJ, 7 Hz and 60 J total energy), followed by root canal sealing as in group I. RESULTS: The data showed lower levels of apical microleakage in the teeth filled with N-Rickert, Sealapex and Sealer 26 cements than in those sealed with Endofill (p < 0.01). No significant difference in microleakage was observed between teeth irrigated only with 0.5% sodium hypochlorite and those submitted to Er:YAG laser application (p > 0.05). CONCLUSIONS: The Er:YAG laser irradiation applied to the root canal walls was not able to prevent apical microleakage.

scholarly journals A comparative study on the effect of household water treatment on diarrhoea morbidity among under-fives in Plateau State, Nigeria using two treatment modalities

2020 ◽  
Vol 8 (05) ◽  
pp. 1789-1799
Author(s):  
Elizabeth Onyi Okoh ◽  
Esther Awazzi Envuladu ◽  
Chundung Asabe Miner ◽  
Amina Mohammed ◽  
Gloria Nengi Ode ◽  
...  
Keyword(s):  
Water Treatment ◽  
Rural Communities ◽  
Sodium Hypochlorite ◽  
Intervention Group ◽  
Control Group ◽  
Sodium Hypochlorite Solution ◽  
Household Water Treatment ◽  
Significant Difference ◽  
Household Water ◽  
Plateau State

Background: Consumption of unsafe water contributes to diarrhoeal burden in sub-Sahara Africa. This burden can be reduced by simple and affordable methods of household water treatment. The study aims at comparing the effect of two of such methods on diarrheal burden among under-fives in rural communities of Plateau State. Methodology: A community-based quasi experimental study was conducted among 202 underfive-caregiver pairs in two selected rural communities of Plateau State, involving household water treatment with flocculant-disinfectant powder in intervention group and sodium hypochlorite solution in control group respectively. History of diarrhoea before intervention, at 2 weekly intervals during the intervention and after intervention was assessed. Data obtained was analyzed using SPSS version 23. Prevalence and incidence of diarrhoea were calculated and compared among the two groups. Result: Diarrhoea prevalence at pre-intervention was 19.6% in flocculant-disinfectant group and 17.0% in sodium hypochlorite group. At post intervention, the prevalence reduced significantly by 94.7% (19.6% to 1%) in intervention group and by 76.5% (17% to 4.2%) in control group. There was no statistical significant difference in the prevalence of diarrhoea between the two groups, however, diarrhoea incidence in the sodium hypochlorite group (1.12 episode per child per year) was significantly higher than the incidence in the flocculant-disinfectant group (0.59 episodes per child per year) with a  risk-ratio of 1.93 (95% CI: 1.037 - 3.703). Conclusion: Household water treatment with flocculant-disinfectant reduced diarrhoea burden better than sodium hypochlorite. This technology should be made more available and assessible to rural communities where diarrhea burden is high.

scholarly journals Hubungan Pemberian Ekstrak Cordyline fruticosa L.A Cheval Terhadap Penurunan Kolesterol Tikus Putih

2020 ◽  
pp. 152
Author(s):  
Dian Rahmawati
Keyword(s):  
Total Cholesterol ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Cholesterol Levels ◽  
White Rats ◽  
Group I ◽  
Significant Difference ◽  
Cordyline Fruticosa

ABSTRACT This study aims to determine the effect of ethanol extract of cordyline fruticose leaves in reducing total cholesterol levels of male white rats. This study used 30 male white rats which were divided into 6 treatment groups, each groups consistent of 5 test animals. Group I was normal control, group II was negative control (High cholesterol died and streptozotocin 30 mg/kgBW, group III was positive control (Simvastatin suspension), group IV, V, VI were each given an ethanol extract of cordyline fruticose leaves at a dose of 100 mg/kgBW, 150 mg/kgBW and 200 mg/kgBW for 14 consecutive days. Measurements of analyzed using a one-way statistical test (ANOVA) at 95% confidence level and continued with the Least Significant Difference (LSD) test to analyze differences between the groups. The result showed that the ethanol extract of cordyline fruticose leaves at a dose 200 mg/kgBW was able to reduce total cholesterol levels in white rats with cholesterol level of 39 mg/dL. Keywords: High fat feed, Cordyline fruticosa, Cholesterol.

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