scholarly journals BLACK AND RED ONCOM EXTRACT SUPPLEMENTATION LENGTHEN ESTROUS CYCLE AND THICKEN ENDOMETRIUM OF WHITE RATS

2020 ◽  
Vol 21 (4) ◽  
pp. 558-564
Author(s):  
Hanifah Alshofa Nurul Aini ◽  
Desak Nyoman Dewi Indira Laksmi ◽  
Ni Luh Eka Setiasih ◽  
Steven Dwi Purbantoro
Keyword(s):  
Estrous Cycle ◽  
Feeding Tube ◽  
Vaginal Swab ◽  
Female Rats ◽  
Control Group ◽  
Antral Follicles ◽  
White Rats ◽  
Fertile Female ◽  
Group I ◽  
Significant Difference

hytoestrogens are active compounds, derived from plants, which have a similar structure and function as estrogen. Phytoestrogens are commonly found in legumes. Oncom, which is assumed containing phytoestrogens, is one of the most famous legumes food from Indonesia and widely consumed daily in West Java. This study was aimed to determine the effect of oncom extract on estrous cycle, endometrium thickness, and the number of antral follicles in productive age rats (Rattus novergicus). This experimental study was using 21 three-to-four-month-old fertile female rats and divided into three groups. Group I (K) was considered as a control group without any treatment. Group II and III were treatment groups which were given black (H) and red (M) oncom extracts 0.005 g/g body weight, respectively, orally with a feeding tube for 14 days. The length of the estrous cycle was measured by performing vaginal swab with interval 12 hours after first treatment was given and during the treatment. Endometrium thickness and the number of antral follicles were measured by collecting the organs uterus and ovary for histological purpose with paraffin method after rats were euthanized post-treatment oncom extracts for 14 days. Data were analyzed by ANOVA and continued with LSD test. The total length of estrous cycle of control group, black oncom extract group, and red oncom extract group was 107,43±3,16 hours, 141,43±15,36 hours, and 161,14±17,10 hours, respectively. The mean of endometrium thickness of control group, black oncom extract group, and red oncom extract group was 346,945±65,88 ?m, 485,740±86,69 ?m, and 533,904±78,93 ?m, respectively. The number of antral follicles of control group, black oncom extract group, and red oncom extract group was 6,00±1,54, 8,43±2,99, and 9,14±2,72, respectively. Results showed that black and red oncom extracts had a significant effect on the length of estrous cycle and endometrium thickness in rats, yet there is no significant difference in the number of antral follicles. In summary, black and red oncom extracts had effects on the length of estrous cycle and endometrium thickness, yet there was no effect on the number of antral follicles.

scholarly journals Embryotoxic and teratogenic effects of ‘Vitosept’ on white rats

2019 ◽  
Vol 5 (4) ◽  
pp. 22-26
Author(s):  
M. P. Soltys ◽  
H. V. Rudyk ◽  
V. M. Gunchak ◽  
B. V. Gutyi
Keyword(s):  
Sodium Hypochlorite ◽  
High Purity ◽  
Female Rats ◽  
Control Group ◽  
Sodium Hypochlorite Solution ◽  
Teratogenic Effects ◽  
Pregnant Females ◽  
White Rats ◽  
Group I ◽  
Significant Difference

The effect of ‘Vitosept’ drug, based on sodium hypochlorite solution with high purity, obtained in a specially developed membraneless flow electrolyzer, on the embryotoxic and teratogenic properties in rats was investigated. To determine the embryotoxic effect of ‘Vitosept’ on the development of white rats offspring of the 1st generation, control and three experimental groups (G1, G2, G3) were formed from pregnant females. The females of the control group with a blunt probe were injected daily for 30 days with 5 ml of isotonic sodium chloride solution, and experimental ones with 5 ml of ‘Vitosept’ drug with different concentration of high purity sodium hypochlorite: Group I (G1) — 50 mg/l; Group II (G2) — 100 mg/l; Group III (G3) — 500 mg/l. The animals were observed. During the observation the condition and behavior of the females, the dynamics of body weight change, duration of pregnancy, and the course of birth were monitored. The results of the experiment were recorded after the slaughter of pregnant females (20th day of pregnancy) and in the postnatal period of development of the offspring. Studies have shown that the use of different concentrations of the drug ‘Vitosept’ in rats for 30 days before and during pregnancy has no embryotoxic and teratogenic effects. According to the indicators of the total, pre- and postimplantation lethality of embryos, there were no reliable changes in the structure and morphometry of internal organs and tissues in 20-day-old fetuses, and their development corresponded to the terms of pregnancy. There was no significant difference between the fertility of female rats in the test and control groups. The average number of fetuses per female was within 9 animals. The rats obtained from the females of the experimental groups were viable and did not lag behind in growth and development compared with the control animals, which generally characterizes the studied drug ‘Vitosept’ as non-toxic, lacking embryotoxic and teratogenic action

scholarly journals EFEKTIVITAS EKSTRAK ETANOL DAUN EFEKTIVITAS EKSTRAK ETANOL DAUN SIDAGURI (Sida rhombifolia L.) TERHADAP PENURUNAN KADAR ASAM URAT PADA TIKUS JANTAN GALUR WISTAR HIPERURISEMIA

2020 ◽  
Vol 3 (1) ◽  
pp. 29-37
Author(s):  
Bunga Rimta Barus
Keyword(s):  
Body Weight ◽  
Uric Acid ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Weight Group ◽  
White Rats ◽  
Group I ◽  
Significant Difference ◽  
Sida Rhombifolia

Sidaguri leaves (Sida rhombifolia L.) Have been shown to contain flavonoid compounds, where they are efficacious as hyperuricemia. This study aims to determine effectiveness ethanol extract of sidaguri leaves as antihyperuricemia in white rats. The study was conducted using the method of potassium oxanate induction and measured using glucometers. 15 animals were divided into 5 groups, namely negative control, positive control, group I combination of ethanol extract of sidaguri leaves 50 mg / kg body weight, group II ethanol extract of sidaguri leaves 100 mg / kg body weight, group III ethanol extract of sidaguri leaves 200 mg / kg body weight for 5 hours. Examination of antihyperuricemia effects is done by comparing the uric acid levels value of the rats before and after treatment. The results of this study indicate that the three dose ethanol extract of sidaguri leaves provide an antihyperuricemia effect and based on one-way Anova, Tuckey and LSD data analysis, shows that the dose of 50 mg / kg body weight is effective as antihyperuricemia and does not have a significant difference (p> 0.05). The conclusion of this study is that the dose is the most effective in decreases uric acid levels in white rats, which is a dose of 200 mg / kg body weight because it mostly decreased uric acid levels.

scholarly journals Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study

2021 ◽  
Author(s):  
Abdul Salam Ansari ◽  
Kiran Sevliya ◽  
Ayesha Badar ◽  
Nirmal Kumar Lohiya
Keyword(s):  
Experimental Study ◽  
Estrous Cycle ◽  
Fallopian Tube ◽  
Female Rats ◽  
Control Group ◽  
Reversible Inhibition ◽  
Tubal Occlusion ◽  
Group I ◽  
Single Intervention ◽  
Group Ii

Background: Reversible inhibition of sperm under guidance (“RISUG®”) is a promising intravasal male contraceptive. Objective: An exploratory study was conducted with a concept of non-invasive, transcervical, single-intervention and reversible contraception using RISUG® in females. Materials and Methods: In this experimental study, 60 adult Wistar albino female rats weighing 150-155 g, 3-4 months old were divided into four groups: group I: sham-operated control; group II: tubal occlusion with RISUG for 90 days; group III: tubal occlusion with RISUG® for 90 days and reversal with dimethyl sulphoxide and group IV: tubal occlusion with RISUG® for 90 days and reversal with 5% NaHCO3. Animals were subjected to bilateral fallopian tube occlusion with RISUG® and reversal with DMSO and NaHCO3. The estrous cycle, fertility and histology of fallopian tube were evaluated. Results: Group I showed 100% fertility during all mating schedules. Animals of experimental groups indicated positive mating, but 0% fertility was evident following 30, 60, and 90 days of tubal occlusion. However, after reversal, fertility steadily increased to normalcy in groups III (50% at 45 days, 80% at 105 days, 100% at 150 and 195 days) and IV (70% at 45 and 105 days, 100% at 150 and 195 days) animals. Group II illustrated disorganized inner cell linings and eosinated RISUG® implant-filled lumen. Reversal groups (III and IV) revealed complete restoration of cellular histo-architecture. Regular estrous cycle was noticed in all experimental groups. Conclusion: RISUG® is suitable for single intervention, intratubular, reversible contraception in female rats. Key words: Contraception, Fallopian tube, Tubal occlusion, RISUG®, Reversible.

scholarly journals Hubungan Pemberian Ekstrak Cordyline fruticosa L.A Cheval Terhadap Penurunan Kolesterol Tikus Putih

2020 ◽  
pp. 152
Author(s):  
Dian Rahmawati
Keyword(s):  
Total Cholesterol ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Cholesterol Levels ◽  
White Rats ◽  
Group I ◽  
Significant Difference ◽  
Cordyline Fruticosa

ABSTRACT This study aims to determine the effect of ethanol extract of cordyline fruticose leaves in reducing total cholesterol levels of male white rats. This study used 30 male white rats which were divided into 6 treatment groups, each groups consistent of 5 test animals. Group I was normal control, group II was negative control (High cholesterol died and streptozotocin 30 mg/kgBW, group III was positive control (Simvastatin suspension), group IV, V, VI were each given an ethanol extract of cordyline fruticose leaves at a dose of 100 mg/kgBW, 150 mg/kgBW and 200 mg/kgBW for 14 consecutive days. Measurements of analyzed using a one-way statistical test (ANOVA) at 95% confidence level and continued with the Least Significant Difference (LSD) test to analyze differences between the groups. The result showed that the ethanol extract of cordyline fruticose leaves at a dose 200 mg/kgBW was able to reduce total cholesterol levels in white rats with cholesterol level of 39 mg/dL. Keywords: High fat feed, Cordyline fruticosa, Cholesterol.

scholarly journals Regenerative Therapy Modality for Treatment of True Combined Endodontic-Periodontal Lesions: A Randomized Controlled Clinical Trial

2021 ◽  
Vol 18 (12) ◽  
pp. 6220
Author(s):  
Reham AlJasser ◽  
Sundus Bukhary ◽  
Mohammed AlSarhan ◽  
Dalal Alotaibi ◽  
Saleh AlOraini ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Grafting ◽  
Endodontic Treatment ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Bony Defect ◽  
Keratinized Tissue ◽  
Group I ◽  
Significant Difference ◽  
Grafting Procedure

The aim of this in-vivo study was to evaluate/compare the clinical periodontal parameters in patients with true combined endo-perio lesions (EPL), treated with gutta-percha (GP) and mineral trioxide (MTA) as an obturation material alone and with addition of bone grafting in such lesions. 120 Saudi patients (mean age = 41yrs) diagnosed with true combined EPL participated in this study. Group I (control group, n = 30) was treated with conventional endodontic treatment using GP for obturation. Group II (n = 30) was treated with conventional endodontic treatment using MTA for obturation. Group III (n = 30) was treated with conventional endodontic treatment using GP for obturation + grafting procedure to fill the bony defect. Group IV (n = 30) was treated with conventional endodontic treatment using MTA for obturation + grafting procedure to fill the bony defect. Clinical parameters (Pocket depth (PD); Clinical attachment loss (CAL); keratinized tissue width (KTW); gingival phenotype (G.Ph.) and Cone Beam Computed Tomography Periapical Index (CBCTPAI)) were recorded and compared at baseline, 3, 6, 12 months’ interval. For the groups III and IV, CBCTPAI showed significant difference (p < 0.0001) with the other groups at 6 months and 1-year interval. The group with MTA + bone graft showed 76% and 90% patients with 0 score at 6 months and 1-year follow-up, respectively. Comparison of mean values of PD among study groups at 3 months, 6 months and 1 year showed significant difference at 3 months, whereas the mean PD values of subjects in GP + bone graft showed significantly higher PD values than other 3 groups (p = 0.025). Use of GP and MTA for root canal obturation along with periodontal therapy and bone augmentation helps in resolving complex endo-perio lesions. Bone grafting in addition to obturation with MTA was found to be the best treatment strategy in management of EPL cases and is recommended for clinicians who are treating EPL patients.

scholarly journals Comparative Evaluation of Fracture Resistance of Endodontically Treated Teeth Restored with Resin Fiber Post and Stainless Steel Post: An In Vitro Study

2015 ◽  
Vol 03 (02) ◽  
pp. 080-084
Author(s):  
Vijay Singh ◽  
Poonam Bogra ◽  
Saurabh Gupta ◽  
Navneet Kukreja ◽  
Neha Gupta
Keyword(s):  
Stainless Steel ◽  
Fracture Resistance ◽  
Testing Machine ◽  
Compressive Force ◽  
Control Group ◽  
Fiber Post ◽  
Endodontically Treated Teeth ◽  
Group I ◽  
Significant Difference

AbstractFracture resistance of endodontically treated teeth restored with post. Aims: This study aims to compare the fracture resistance of endodontically treated teeth restored with resin fiber and stainless steel post. Commercially available prefabricated resin fiber post(Dentsply Maillefer Easy Post), prefabricated stainless steel post(Coltene/Whaledent Parapost) were used. Methods and Material: Forty five maxillary central incisors were obturated and divided into 3 groups: Control Group (Group I) without any post (n = 15), Resin Fiber Post Group (Group II) (n = 15) and Stainless Steel Post Group (Group III) (n = 15). In all Groups except control group, post space was prepared; a post was cemented, and a core build-up was provided. All the specimens were subjected to compressive force under a universal testing machine until fracture. Statistical analysis used: The results were analyzed using the variable analysis test (ANOVA). Results: One-way analysis of variance revealed significant difference among test groups. The control group demonstrated highest fracture resistance (925.2183 N), followed by the resin fiber post group (486.7265 N) and stainless steel post group (423.539N). Conclusions: Teeth restored with resin fiber post showed higher fracture resistance values than prefabricated stainless steel post.

Should fertile women quit drinking alcohol to produce better quality oocytes?

Zygote ◽  
2020 ◽  
pp. 1-3
Author(s):  
Burcu Ozbakir ◽  
Pinar Tulay
Keyword(s):  
Alcohol Consumption ◽  
Young Women ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Control Group ◽  
Social Drinkers ◽  
Antral Follicles ◽  
Healthy Women ◽  
Significant Difference

Summary Alcohol consumption has long been shown to affect both fetal health and pregnancy. In this study, antral follicle count, maturation level of oocytes including morphological assessment and number of metaphase I (MI), metaphase II (MII) and germinal vesicle (GV) stage oocytes obtained from young women (age < 30 years old) with or without alcohol consumption were investigated. In total, 20 healthy women who were social drinkers and 36 healthy women who do not consume alcohol were involved in this study. Women in both study and control groups were undergoing controlled ovarian stimulation. The antral follicle count and the number and quality of the oocytes retrieved were evaluated and recorded. In total, 635 antral follicles, 1098 follicles and 1014 oocytes with 820 MII, 72 MI and 78 GV stage oocytes were collected from the social drinkers. In the control group, 628 antral follicles, 1136 follicles and 1085 oocytes with 838 MII, 93 MI and 102 GV stage oocytes were evaluated. The results of this study showed that the antral follicle count was very similar in both groups. The number of oocytes and MII stage oocytes was slightly higher in the control group, although it was not a significant difference. This study showed that although the consumption of alcohol may have adverse effects post-implantation, it may not have a solid effect during oogenesis in young women. The results of this study are especially important in clinical settings as some women who are social drinkers undergo in vitro fertilization treatments.

Histological Study On Using Ileal Submucosa In Repairing Urinary Bladder Defect In Adult Albino Rat

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Eman Gomaa El Saeed ◽  
Manal H Moussa ◽  
Gehad A Hammouda ◽  
Sahar M. M Omar
Keyword(s):  
Urinary Bladder ◽  
Histological Study ◽  
Squamous Metaplasia ◽  
Muscle Layer ◽  
Control Group ◽  
Albino Rats ◽  
Adult Rats ◽  
Group I ◽  
Significant Difference ◽  
Graft Area

Abstract Background Repairing urinary bladder (UB) defect by enterocystoplasty remains the gold standard surgical bladder reconstruction procedure to increase the capacity and compliance of dysfunctional bladders. However, many complications were recorded. Aim of the work This work aimed to compare the consequences of reconstruction of urinary bladder defect using untreated small intestinal submucosal (SIS) matrix versus seeded and unseeded decellularized SIS matrix. Material and Methods Fifty female albino rats were used in this study. The animals were divided into three groups: Group I (Control) included ten adult rats from which ileal tissue was obtained. Group II included ten adult rats in which their UB defect was repaired by untreated cellular SIS. Group III included twenty adult rats that were subdivided into two subgroups, 10 rats each; Subgroup IIIA where rats had their UB defect repaired by acellular SIS and subgroup IIIb where rats had their UB defect repaired by acellular SIS seeded with adipose mesenchymal stem cells (AMSCs).Ten young rats were used for preparation of AMSCs. Morphometric and statistical analysis were also performed. Results In rats where UB defect was repaired by untreated cellular SIS, the graft area showed loss of epithelial polarity, presence of intraepithelial cysts and occasional extension of urothelium to the outer surface forming fistula. There were areas of metaplasia with the appearance PAS positive cells. In the lamina propria, there was areas of lymphocytic infiltration together with significant increase in the collagen fiber deposition (p &lt; 0.05). There was a significant decrease thickness of muscle layer as compared to control (p &lt; 0.05). In rats where UB defect was repaired by acellular SIS, urothelium in the graft area showed occasional squamous metaplasia and often the urothelium extended to the deeper layers forming Brunn's nest. There was minimal muscle regeneration in the graft area. However, in rats where UB defect was repaired by acellular SIS seeded with AMSCs, the urothelium in the graft area was nearly similar to control group with uniform urothelium thickness, minimal collagen fibers deposition and thick muscle layer that showed no significant difference from the control (p &gt; 0.05). Conclusion Acellular SIS seeded with AMSCs showed better results compared to non-seeded and cellular SIS in reconstructing urinary bladder defects.

Impact of supplementing commercial rabbit pellets with fresh Moringa oleifera leaves on semen characteristics in rabbit bucks under tropical climate in Trinidad

2021 ◽  
Author(s):  
Krishna Mohan Kumar
Keyword(s):  
Sperm Motility ◽  
Moringa Oleifera ◽  
Sperm Count ◽  
Control Group ◽  
Computer Assisted ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Moringa Oleifera Leaves ◽  
The Impact

Objective This study aimed to evaluate the impact of the dietary supplement of Moringa oleifera leaves (MOL) on semen quality and characteristics in rabbits. Methods Eighteen (n=18) breeding bucks of New Zealand white, of similar age group, were used for the study. Three feeding regimes, (i) 100% commercial rabbit pellets (CRP)-Group I (ii) 90% CRP + 10% fresh MOL on a dry matter (DM) basis – Group II and (iii) 80% CRP + 20% fresh MOL on a DM basis – Group III, were adopted and the trial continued for 21 days. After adaptation to the diet, semen was collected from each buck and subjected to evaluation using a computer-assisted semen analyser. Results In Group III, the sperm count, normal sperm morphology, and sperm motility increased (52.0%) in comparison with the control (Group I; 50.1%). The inclusion of 20% Moringa oliefera in the diet (Group III) caused a significant increase (P<0.05) in semen concentration (Control =136.2 M/mL; Group III=297.2 M/mL). There was no significant difference (P>0.05) in sperm motility and semen volume among the groups. Conclusion The results suggest that supplementing commercial rabbit pellets with 20% fresh Moringa oliefera leaves on a DM basis can improve the quality and characteristics of semen in breeding bucks.

scholarly journals Assessment of in Obsessive Compulsive Disorder

2021 ◽  
pp. 105-114
Author(s):  
Sarah Alaa Mohsen ◽  
Fatma Ahmed El Deeb ◽  
Ehab Sayed Ramadan ◽  
Mai Abd El-Raouf Eissa
Keyword(s):  
Obsessive Compulsive Disorder ◽  
Test Performance ◽  
Age Of Onset ◽  
Neuropsychological Test ◽  
Control Group ◽  
Obsessive Compulsive ◽  
Cross Sectional ◽  
Compulsive Disorder ◽  
Group I ◽  
Significant Difference

Background: Obsessive compulsive disorder (OCD) is a common and potentially debilitating disorder. Neuropsychological assessment provides unique complementary information that is critical for evaluating higher cortical abilities. This study aimed to assess the neuropsychological functions in OCD patients which can then point to the brain structures or pathways and to study the correlation between these assessments and different clinical variables. Methods: This cross-sectional case control study had included sixty patients who were divided into two groups, Group I: thirty OCD patients diagnosed by DSM-IV and Group II: thirty healthy controls who were recruited from the community, matched with patients’ age, gender, and education. Results: The age of onset in our study was 19.13 ± 0.35 years, the mean duration was 7.44 ± 3.88 years, 40% of the studied cases had severe OCD symptoms and 33.3% of them were compulsive cleaners. There was a high significant difference between the two groups regarding WCST in favor of the control group. There was a high significant difference between the two groups regarding ROCF where the control group showed better results than the OCD patients. Conclusions: Neuropsychological test performance remains an informative and objective means of investigation, especially when applied to psychiatric disorders. The executive functions in OCD patients were impaired in comparison to the normal study subjects.

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