scholarly journals EFFECT OF LACTOFERRIN ADDITION TO THE SEMEN OF AWASSI RAMS ON SOME PHYSICAL PROPERTIES OF SPERMS

2016 ◽  
Vol 47 (6) ◽  
Author(s):  
Mahdi & Houbi
Keyword(s):  
Sperm Concentration ◽  
Average Weight ◽  
College Of Agriculture ◽  
Treatment Groups ◽  
Significant Difference ◽  
Semen Preservation ◽  
Live Sperm ◽  
Motility Of Sperm ◽  
The Individual ◽  
Artificial Vagina

This experiment was conducted at the Animal Production Department farm to the College of Agriculture, University of Baghdad, located at Jadreya during the period of 21 September 2015 to 24 February 2016 using Four Awasi rams aged 2-4 years and the average weight was 52 kg. Semen collection from rams was done twice weekly by using the artificial vagina to study the effect of using the lactoferrin protein as a replacement by the antibiotic in the semen for controlling the contamination of bacteria in the semen. Four treated groups were used, the control (normal antibiotic Streptomycin 100 mg\100ml Penicillin 100.000 IU / 100ml) , second ,third and fourth treatment groups was used a lactoferrin  protein with the contamination of 400, 800 and 1200 mg \ L-1 , respectively . All additions of the antibiotic and lactoferrin were done to the semen after dilution. Semen from all rams was pooled and the final volume was divided by four parts to groups .The semen diluent was kept at the refrigerator 5 C for 5 days, and every day the physical characteristic was done which included. The individual motility, dead and abnormal sperm, concentration of sperm, mass activity, integrity of the plasma membrane (HOST %) The results showed that: the individual motility of sperm was significantly increased) for all treated groups on the second, third, fourth and the fifth day of semen preservation. No significant difference  in the percentage of live sperm as a result of adding lactoferrin to the semen. There were no significant increase) in the hyper osmotic sperm test (HOST%) between the control and treatment groups. The abnormality of sperm did not differ between the control and the treated groups.

scholarly journals SEASONAL VARIATIONS IN SEMINAL CHARACTERISTICS OF WEST AFRICAN DWARF SHEEP IN THE HUMID TROPICS

2021 ◽  
Vol 15 ◽  
pp. 43-47
Author(s):  
J. U. AKPOKODJE ◽  
T. I. DEDE ◽  
P. I. ODILI
Keyword(s):  
Seasonal Changes ◽  
Sperm Concentration ◽  
West African ◽  
West African Dwarf ◽  
Significant Difference ◽  
One Year ◽  
Motility Of Sperm ◽  
Artificial Vagina ◽  
West African Dwarf Sheep ◽  
Dwarf Sheep

Semen collected from six mature West African Dwarf (WAD) rams by artificial Vagina, twice weekly for one year, early and late rainy season, and early and late dry season, showed no significant difference in volume of ejaculate and progressive motility of sperm. There was significant difference in sperm concentration, total sperm per ejaculate, total motile sperm per ejaculate and abnormal sperm. Seasonal changes in characteristics of the semen were associated with seasonal changes in temperature, indicating the detrimental effects of heat, even on indigenous stock.

scholarly journals EFFECT OF LACTOFERRIN ADDTION TO SEMEN OF AWASSI RAMS ON THE BACTERIAL CONTAMINATION

2016 ◽  
Vol 47 (6) ◽  
Author(s):  
Mahdi & Houbi
Keyword(s):  
Staphylococcus Epidermidis ◽  
Control Group ◽  
Average Weight ◽  
College Of Agriculture ◽  
Significant Difference ◽  
Acrosome Integrity ◽  
Semen Extender ◽  
Group 2 ◽  
Number Of Bacteria ◽  
Artificial Vagina

This study was conducted at the farm of Animal production, College of Agriculture, University of Baghdad, Algaderia to know the effect of lactoferrin protein addition in the semen extender of Awassi during the  period from 21 September 2015 to 24 February 2016 on Four Awasi rams aged 2-4 years and the average weight was 52 kg were used in this study . Semen collection from rams was done twice weekly by using the artificial vagina then it's were pooled and divided in to four treated groups, the control (normal antibiotic Streptomycin 100 mg\100ml Penicillin  100.000 IU / 100ml) , second ,third and fourth treated groups was used a lactoferrin  protein with the contamination of 400 , 800 and 1200 mg \ L-1, respectively .After dilution the semen samples have been kept at the refrigerator (5 C) for 5 days to measure some physical properties of sperm which included the acrosome integrity and some bacteriological examination (type and number) .Data obtained from this experiment showed that there was no significant difference (p≥0.05) in the acrosome integrity of sperm between the control and treated groups (2,3 and 4) which were decrease  the most types of bacteria in the semen samples , where as the type of bacteria in group 2 were sphingomonas and Stenotrophomonas  and in groups 3 and 4 were Klebsiella, Proteus, Cupriavidus, while there were six types of bacteria in the control group (Escherichia, Klebsiella, Staphylococcus, Salmonella, Enterobacter , and  Staphylococcus epidermidis ) . It was concluded that the use of lactoferrin in the semen causing adecrease in the total number of bacteria in the semen.

A comparison of ram semen collected by the artificial vagina and by electro-ejaculation

1962 ◽  
Vol 2 (4) ◽  
pp. 78 ◽  
Author(s):  
PE Mattner ◽  
JK Voglmayr
Keyword(s):  
Wave Motion ◽  
Sperm Concentration ◽  
Ph Values ◽  
Normal Sperm ◽  
Live Sperm ◽  
Artificial Vagina

Seven samples obtained alternately by the use of an artificial vagina and by electro-ejaculation from nine rams were compared. Electro-ejaculation resulted in higher volume, fructose and pH values but lower sperm concentration and wave motion. The proportion of live sperm, of morphologically normal sperm, and of actively progressing sperm were similar for both methods. The variation in semen characters, both within and between rams was, in general, slightly higher for electro-ejaculation than for artificial vagina collections. Within-ram variation in total number of sperm was much larger with electro-ejaculation than with collections made using the artificial vagina. For studies of fructose concentration or the total amount of fructose per ejaculate, the artificial vagina method possesses no advantage over electro-ejaculation.

scholarly journals Effect of genotype on semen quality traits of main and reciprocal crossbred chickens

2020 ◽  
Vol 46 (2) ◽  
pp. 32-39
Author(s):  
U. C. Isaac ◽  
U. H. Udoh ◽  
R. J. Nosike
Keyword(s):  
Reciprocal Cross ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Genetic Constitution ◽  
Quality Traits ◽  
Breeding Method ◽  
Naked Neck ◽  
Significant Difference ◽  
Live Sperm

Semen quality of cocks is of utmost importance in reproduction. Breeding method, breed, strain and genetic constitution are the major components of the genetic factors capable of affecting the semen quality of cocks. This research seeks to determine the effect of genotype on the semen quality traits of 272 F crossbred cocks produced at day-old by main and 1 reciprocal crossbreeding of 69 Isa Brown and local frizzle feathered, naked neck and normal feathered chickens. The genotypes of the cocks were Isa Brow x frizzle feathered main cross (IBxF), Isa Brown x naked neck main cross (IBxNa), Isa Brown x normal feathered main cross (IBxN), frizzle feathered x Isa Brown reciprocal cross (FxIB), naked neck x Isa Brown reciprocal cross (NaxIB) and normal feathered x Isa Brown reciprocal cross (NxIB). At 36-40 weeks of age, semen was extracted from the cocks by abdominal  massage technique and used to evaluate semen volume (SV), sperm motility (SM), sperm concentration (SC) and live sperm (LS) at weekly intervals. Data obtained from the evaluation were subjected to analysisof variance and tested at 5% level of probability. Genotypes differed significantly (P <0.05) in SC at 37-40 weeks and in SM and LS at all ages. There was no significant difference (P>0.05) in SV. Regardless of genotype and age, the mean SV, SM, SC and LS ranged from 0.31±0.02 to 0.24±0.01ml, 71.84±1.33 to 58.75±2.16 %, 3.22±0.02 to2.92±0.02 x109/ml and 71.91±1.37 %, respectively. The NxIB and IBxNa genotypes produced largest numericalsemen volume at 37 and 39 weeks of age respectively. Whereas SC and LS were significantly highest for IBxF and IBxN respectively at week 37, SM was so for IBxN at all ages. Further, the LS of IBxN genotype was significantly highest at 36-38 and 40 weeks of age. The exotic Isa Brown and normal feathered main cross cocks exhibited the best semen quality traits among all genotypes studied and should be selected for improvement of the reproductive characteristics of cocks.  

scholarly journals Anti-Inflammatory Effects ofInonotus obliquusin Colitis Induced by Dextran Sodium Sulfate

2010 ◽  
Vol 2010 ◽  
pp. 1-5 ◽  
Author(s):  
Se Young Choi ◽  
Sun Jin Hur ◽  
Chi Sun An ◽  
Yun Hui Jeon ◽  
Young Jun Jeoung ◽  
...  
Keyword(s):  
Sodium Sulfate ◽  
Dextran Sodium Sulfate ◽  
Raw264.7 Cells ◽  
Average Weight ◽  
Inonotus Obliquus ◽  
Signal Transducers ◽  
Commercial Diet ◽  
Treatment Groups ◽  
Significant Difference ◽  
Group B

A total of 28 male BALB/c mice (average weight 20.7 ± 1.6 g) were divided into 4 treatment groups and fed a commercial diet (A), a commercial diet + induced colitis by dextran sodium sulfate (DSS) (B),Inonotus obliquus(IO) administration (C), and IO administration + induced colitis by DSS (D). IO treatment (C, D) decreased the expression of tumor necrosis factor (TNF)-αand signal transducers and activators of transcription (STAT)1 compared to those of the colitis induced group (B). The expressions of IL-4 and STAT6 were decreased in group D compared to the colitis induced group (B). The serum immunoglobulin (Ig)E level decreased in IO treatment groups (C, D) compared to no IO treatment groups (A and B) although there was no significant difference between the IO treatment groups. Extract from IO itself had a weak cytotoxic effect on murine macrophage cell line (RAW264.7 cells). Extract from IO inhibited lipopolysaccharide- (LPS-) induced, TNF-α, STAT1, pSTAT1, STAT6, and pSTAT6 production in RAW264.7 cells.

scholarly journals PENGARUH PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum) TERHADAP DIAMETER DAN TEBAL EPITEL TUBULUS SEMINIFERUS MENCIT (Mus musculus) YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN

2020 ◽  
Vol 8 (2) ◽  
pp. 132
Author(s):  
Aldis Ingrid Rusyawardani ◽  
Widjiati Widjiati ◽  
Suzanita Utama ◽  
Chairul Anwar ◽  
Epy Muhammad Luqman ◽  
...  
Keyword(s):  
Single Dose ◽  
Mus Musculus ◽  
Seminiferous Tubules ◽  
Average Weight ◽  
Male Mice ◽  
Treatment Groups ◽  
Epithelial Thickness ◽  
Significant Difference ◽  
Biophytum Petersianum ◽  
Epithelium Thickness

The aim of this research is to analyze the effect of kebar grass on the diameter and epithelium thickness of seminiferous tubules. Twenty male mice of BALB/C strain with an average weight of 25 gram were used in this research. Total five experimental groups (4 mice each group) were treated with combination of TCDD and kebar grass extract designed as follows: (C-) 0mg/g BW/day dan 0µg/kg BW, (C+) 0mg/g BW/day and 7µg/kg BW, (T1) 0,045mg/g BW/day and 7µg/kg BW, (T2) 0,080mg/g BW/day and 7µg/kg BW, (T3) 0,1350mg/g BW/day and 7µg/kg BW. The TCDD was injected peritoneally in single dose while kebar grass extract was given orally everyday for 53 days. The results showed a significant difference (p<0,05) on group which is treated with kebar grass extract 0,135mg/ g BW/ day is proven could maintain the diameter of seminiferous tubules in T3 group (170,5μmb ± 0,65μm) compared with C+ group (129,50μma ± 8,42μm). Significant difference of epithelial thickness were also found between the control and treatment groups (p<0,05). The C+ group (33,57μmb ± 1,61μm) was different from the T1 group (43,80μmc ± 1,46μm), T2 (48,49μmd ± 1,08μm), T3 (52,15μme ± 0,86μm) and C- (56,17μma ± 1,35μm). This findings might suggest TCDD has the potential to decrease the epithelium thickness and the diameter of seminiferous tubules, while kebar grass extract in various dose were proven to be able maintain the epithelium thickness of seminiferous tubules but could not maintain the diameter of seminiferous tubules.

scholarly journals FREEZING CAPABILITY OF PASUNDAN BULL SPERM USING TRIS-EGG YOLK, TRIS-SOY, AND ANDROMED® DILUENTS

2017 ◽  
Vol 11 (1) ◽  
pp. 45-49
Author(s):  
Abdullah Baharum ◽  
R. Iis Arifiantini ◽  
Tuty Laswardi Yusuf
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Freezing Process ◽  
Sperm Abnormalities ◽  
Bull Sperm ◽  
Motility Of Sperm ◽  
Artificial Vagina

The aims of this study were to investigate the freezing capability of Pasundan bull spermatozoa in Tris-egg yolk (TEY), Tris-soy (TS), and AndroMed® as diluents. Semen were collected twice a week from four Pasundan bulls aged 3-5 years old using an artificial vagina and evaluated macro- and microscopically. Semen had ≥70% sperm motility, ≥800x106/mL sperm concentration, and less than 20% sperm abnormalities were divided into three parts and each of them diluted with TEY, TS, or AndroMed®. After an equilibration step at 5°C for four hours, diluted semen were packaged in 0.25 mL straw, frozen in liquid nitrogen for ten minutes and kept in liquid nitrogen container until examination. Motility test on fresh, diluted, equilibrated, and after-thawed semen was done using Androvision®. The results showed that after thawing motility of sperm diluted in AndroMed® (58.64±0.72%) was higher than in TEY (49.45±1.22%) and TS (39.34±6.33%). Sperm motility of Pasundan bulls diluted in these three diluents reduced around 33.27±2.45% during freezing process.

scholarly journals PRELIMINARY OBSERVATIONS ON THE SEMINAL CHARACTERISTICS OF RABBITS RAISED IN TWO LOCATIONS IN SOUTHEASTERN NIGERIA.

2021 ◽  
Vol 22 (2) ◽  
pp. 108-112
Author(s):  
U. Herbert ◽  
C. O. Acha
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Southeastern Nigeria ◽  
Abnormal Sperm ◽  
Port Harcourt ◽  
Semen Volume ◽  
Live Sperm ◽  
Artificial Vagina ◽  
Time Of Collection

A total of sixteen rabbits sourced from two locations in Southeastern Nigeria were used for this study. Semen was collected from all the rabbits twice weekly for eight weeks using an artificial vagina while observation on the effect of time of collection on semen quality were also made. The results obtained indicated that time of collection had no significant effect (P > 0.05) on all the seminal characteristics measured. However, source of the animals showed significant effect (P < 0.05) on semen volume, sperm concentration, total sperm per ejaculate and proportion of abnormal sperm. Average semen volume, sperm concentration and total sperm per ejaculate were: 0.69±0.007 ml, 104.0±1.44 x 106/m1 and 71.40±0.90x106 respectively for the rabbits sourced from Port Harcourt. The corresponding values for rabbits sourced from Owerri were: 0.66±0.008m1, 96.02 ±1.63x106iml and 63.49±1.50 x 106 respectively. Abnormal sperm proportions were 11.05±0.22% and 1216 ± 032% for the rabbits sourced from Port Harcourt and Owerri respectively. There were no significant (P> 0.05) differences observed between locations in live sperm concentration and sperm motility. Live sperm concentration and sperm motility were 87.75±1.09% and 71.62±0.74% for the Port Harcourt group, while the values obtained for the Owerri group were 88.06±034% and 71.19±0.89% respectively.  

scholarly journals Effect of supplementation with organic acids on productive and reproductive parameters in guinea pigs

2020 ◽  
Vol 37 (1) ◽  
pp. 51-58
Author(s):  
Christian Vela ◽  
Siever Morales-Cauti
Keyword(s):  
Organic Acids ◽  
Guinea Pigs ◽  
Production Systems ◽  
Lower Percentage ◽  
Average Weight ◽  
Treatment Groups ◽  
Significant Difference ◽  
Mortality Percentage ◽  
Weight Gain Percentage ◽  
Observation Period

Guinea pig production systems demand organic alternatives to improve their productivity, due to the increased demands of this type of livestock operations. The present study aimed to evaluate the effect of organic acids on the productive and reproductive indices in reproductive Guinea pigs in an intensive breeding farm in Lima. 60 pools were used with 10 Guinea pigs in each one (9 females and one male).  They were randomly divided into 4 treatment groups: T1: 1mL/L, T2: 2mL/L, T3: 4mL/L; all with 15 pools in total (5 pools each with dosage of 5, 10 and 15 days, respectively), and T4: Control (15 pools without dosage); the observation period was 3 months. No significant difference was found (p> 0.05) between treatments for weight gain, percentage of maternal mortality, percentage of abortions, percentage of pregnancy, number of offspring born and percentage of viability. For the percentage of mortality of pups and average weight of the offspring, a significant difference was found (p <0.05), demonstrating that T3 reached a higher weight index at birth of offspring (T3: 147.7 / 142.7 / 146.8g) and lower percentage of mortality in rabbits (T3: 0 / 1.8 / 3.0%). No significant difference was found in terms of dosing days. It is concluded that supplementation with organic acids with T3 (4mL/L) improved the weight at birth of the offspring and the mortality percentage in kits.

scholarly journals Effect of level of monensin on rumen fermentation characteristics in Awassi lambs

2020 ◽  
Vol 11 (2) ◽  
pp. 1-11
Author(s):  
Razzaq Baiee ◽  
◽  
Ali Saeed ◽  
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fermentation ◽  
The Body ◽  
Control Treatment ◽  
Average Weight ◽  
College Of Agriculture ◽  
Experimental Treatments ◽  
The Individual ◽  
Ground Wheat ◽  
Awassi Lambs

The current study was conducted in the Animal field of the Department of Animal Production – College of Agriculture - Al-Qasim Green University for the period from 10/10/2019 to 19/1/2020 to investigate the effect of addition of monensin at levels of 15, 30 and 45 mg/kg concentrate on growth performance of Awassi lambs. First treatment in which lambs were fed concentrate diet without addition of monensin was considered the control treatment. Sixteen male Awassi lambs were used with an average weight of 24.85 and 4-6 months of age. The lambs were randomly distributed to the experimental treatments, four lambs per each using the individual feeding in the cages. The concentrate diet was offered at a level of 2.75% of the body weight at 2 meals, morning and evening meals, while the ground wheat straw was offered at free choice. Results revealed no significant effect of level of monensin on pH values, whereas ruminal ammonia concentrations were significantly decreased (P˂0.05) from 7.54 in the control treatment to 6.08 and 6.45 mg/100 ml due to addition of monensin at levels of 30 and 45 mg/kg. Moreover, addition of monensin at a level of 30 mg/kg concentrate diet significantly increased (P˂0.05) ruminal concentration of total volatile fatty acids to 13.76 as compared with 10.99 mM/100 ml for control treatment. Concentrations of all rumen fermentation characteristics were significantly affected (P˂0.01) by time of ensiling.

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