scholarly journals DETECTION OF THE ANTIMICROBIAL ACTIVITY OF SILVER NANOPARTICULES BIOSYNTHESIZED BY STREPTOCOCCUS PYOGENES BACTERIA

2020 ◽  
Vol 51 (2) ◽  
pp. 500-507 ◽  
Author(s):  
Kadhum & Hussein
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Streptococcus Pyogenes ◽  
X Ray Diffraction ◽  
Pathogenic Microbes ◽  
Vitek 2 ◽  
Uv Visible ◽  
Agar Well Diffusion Assay ◽  
Moderate Range ◽  
Diffusion Assay

This study was aimed to biosynthesized silver nanoparticles by Streptococcus pyogenes bacteria and its antimicrobial activity against (S.aureus ,P. aeruginosa, E.coli, and C. albicans yeast) at different  concentrations (20, 40, 60 ,80 and 100)  μg/ml by agar  well  diffusion  assay. Fifty sample was collected from Wounds and burns, from Baghdad Teaching City Medicine Laboratories. Samples identified by culture, VITEK 2 Compact system ID-YST kit. The sensitivity of bacterial isolates to antibiotics ware tested and the microbes were more  sensitive,  resistant  and  moderate  range to antibiotics. Several techniques where used to characterize AgNPs: X-ray Diffraction (XRD), UV–Visible Spectroscopy(UV) and Scanning electron microscope (SEM).The results show that biosynthesized silver nanoparticles are more effective than bacterial supernatant on human pathogenic microbes.

scholarly journals Antimicrobial Activity of Silver-Treated Bacteria against other Multi-Drug Resistant Pathogens in Their Environment

Antibiotics ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 181 ◽  
Author(s):  
Doaa Safwat Mohamed ◽  
Rehab Mahmoud Abd El-Baky ◽  
Tim Sandle ◽  
Sahar A. Mandour ◽  
Eman Farouk Ahmed
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Bacterial Cell ◽  
Multidrug Resistant ◽  
Polluted Water ◽  
E Coli ◽  
Wound Contamination ◽  
Transmission Electron ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay

Silver is a potent antimicrobial agent against a variety of microorganisms and once the element has entered the bacterial cell, it accumulates as silver nanoparticles with large surface area causing cell death. At the same time, the bacterial cell becomes a reservoir for silver. This study aims to test the microcidal effect of silver-killed E. coli O104: H4 and its supernatant against fresh viable cells of the same bacterium and some other species, including E. coli O157: H7, Multidrug Resistant (MDR) Pseudomonas aeruginosa and Methicillin Resistant Staphylococcus aureus (MRSA). Silver-killed bacteria were examined by Transmission Electron Microscopy (TEM). Agar well diffusion assay was used to test the antimicrobial efficacy and durability of both pellet suspension and supernatant of silver-killed E. coli O104:H4 against other bacteria. Both silver-killed bacteria and supernatant showed prolonged antimicrobial activity against the tested strains that extended to 40 days. The presence of adsorbed silver nanoparticles on the bacterial cell and inside the cells was verified by TEM. Silver-killed bacteria serve as an efficient sustained release reservoir for exporting the lethal silver cations. This promotes its use as a powerful disinfectant for polluted water and as an effective antibacterial which can be included in wound and burn dressings to overcome the problem of wound contamination.

Antimicrobial Activity of Silver Nanoparticles Synthesized by Streptomyces Species JF714876

2012 ◽  
Vol 5 (1) ◽  
pp. 1638-1642 ◽  
Author(s):  
Vidyasagar G M ◽  
Shankaravva B ◽  
R Begum ◽  
Imrose ◽  
Sagar R ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Silver Ions ◽  
Human Beings ◽  
Streptomyces Species ◽  
Force Microscopy ◽  
E Coli ◽  
Extracellular Synthesis ◽  
Atomic Force ◽  
Uv Visible

Microorganisms like fungi, actinomycetes and bacteria are considered nanofactories and are helpful in the production of nanoparticles useful in the welfare of human beings. In the present study, we investigated the production of silver nanoparticles from Streptomyces species JF714876. Extracellular synthesis of silver nanoparticles by Streptomyces species was carried out using two different media. Silver nanoparticles were examined using UV-visible, IR and atomic force microscopy. The size of silver nanoparticles was in the range of 80-100 nm. Antimicrobial activity of silver nanoparticle against bacteria such as E. coli, S. aureus, and dermatophytes like T. rubrum and T. tonsurans was determined. Thus, this study suggests that the Streptomyces sp. JF741876 can produce silver ions that can be used as an antimicrobial substance.

Synthesis of Nanocomposites of V2OO5©Selenium Nanoparticles and Multiwalled Carbon Nanotubes for Antimicrobial Activity

2021 ◽  
Vol 21 (11) ◽  
pp. 5673-5680
Author(s):  
Muthukrishnan Francklin Philips ◽  
Jothirathinam Thangarathinam ◽  
Jayakumar Princy ◽  
Cyril Arockiaraj Crispin Tina ◽  
Cyril Arockiaraj Crispin Tina ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Antimicrobial Activity ◽  
Multiwalled Carbon Nanotubes ◽  
Xrd Analysis ◽  
X Ray Diffraction ◽  
Multiwalled Carbon ◽  
X Ray ◽  
Functionalized Multiwalled Carbon Nanotubes ◽  
Uv Visible ◽  
Micro Organisms

The authors report the preparation of the nanocomposite comprising of vanadium pentoxide (V2O5) and selenium (Se) nanoparticles and functionalized multiwalled carbon nanotubes (MWCNTs) (V2O5@Se NPs/MWCNTs). Since Se NPs possesses extraordinary physicochemical properties including larger surface area with higher adsorption capacity, V2O5 NPs were adsorbed onto Se NPs surface through physisorption process (designated as V2O5@Se NPs). The nanocomposite synthesized hydrothermally was evaluated for its antimicrobial activity. The morphology and microstructure of the nanocomposite were characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis, respectively. Fourier transform infrared spectroscopy (FTIR) and UV-Visible spectroscopy (UV-Vis) were employed to analyze the spectral properties of nanocomposite. The microbicidal efficacy of nanocomposite was tested against Gram-negative (G-)ZGram-positive (G+) bacteria and fungus. This is the first report on the synthesis of V2O5@Se NPs/MWCNTs nanocomposites by chemical method that showed microbicidal effect on micro-organisms. The thiol (-SH) units facilitates the enrichment of V2O5@Se NPs onto MWCNTs surface. Ultimately, it reflects on the significant antimicrobial activity of V2O5@Se NPs/MWCNTs.

scholarly journals A REVIEW: A GREEN APPROACH FOR THE SYNTHESIS OF SILVER NANOPARTICLES AND ITS ANTIBACTERIAL APPLICATIONS

2018 ◽  
Vol 11 (8) ◽  
pp. 74 ◽  
Author(s):  
Shyla Marjorie Haqq ◽  
Amit Chattree
Keyword(s):  
Silver Nanoparticles ◽  
Antimicrobial Properties ◽  
Nanoparticle Synthesis ◽  
Silver Ions ◽  
Multidrug Resistant ◽  
X Ray Diffraction ◽  
Green Approach ◽  
Transmission Electron ◽  
Uv Visible ◽  
Silver Nanoparticle Synthesis

  This review is based on the synthesis of silver nanoparticles (AgNPs) using a green approach which is biofabricated from various medicinal plants. AgNPs were prepared from the various parts of the plants such as the flowers, stems, leaves, and fruits. Various physiochemical characterizations were performed using the ultraviolet (UV)-visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction spectroscopy, transmission electron microscopy, and energy dispersive spectroscopy. AgNPs were also used to inhibit the growth of bacterial pathogens and were found to be effective against both the Gram-positive and Gram-negative bacteria. For the silver to have antimicrobial properties, it must be present in the ionized form. All the forms of silver-containing compounds with the observed antimicrobial properties are in one way or another source of silver ions. Although the antimicrobial properties of silver have been known, it is thought that the silver atoms bind to the thiol groups in enzymes and subsequently leads to the deactivation of enzymes. For the silver to have antimicrobial properties, it must be present in the ionized form. The study suggested that the action of the AgNPs on the microbial cells resulted into cell lysis and DNA damage. AgNPs have proved their candidature as a potential antibacterial against the multidrug-resistant microbes. The biological agents for synthesizing AgNPs cover compounds produced naturally in microbes and plants. Reaction parameters under which the AgNPs were being synthesized hold prominent impact on their size, shape, and application. Silver nanoparticle synthesis and their application are summarized and critically discussed in this review.

scholarly journals Bio Synthesis and Characterization of Silver Nanoparticles Using Lagenaria siceraria Leaf Extract and their Antibacterial Activity

2014 ◽  
Vol 38 ◽  
pp. 35-45 ◽  
Author(s):  
B. Anandh ◽  
A. Muthuvel ◽  
M. Emayavaramban
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Face Centered Cubic ◽  
Lagenaria Siceraria ◽  
Human Pathogens ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Ft Ir ◽  
Uv Visible

The present investigation demonstrates the formation of silver nanoparticles by the reduction of the aqueous silver metal ions during exposure to the Lagenaria siceraria leaf extract. The synthesized AgNPs have characterized by UV-visible spectroscopy, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR) techniques. AgNPs formation has screened by UV-visible spectroscopy through colour conversion due to surface plasma resonance band at 427 nm. X-ray diffraction (XRD) confirmed that the resulting AgNPs are highly crystalline and the structure is face centered cubic (fcc). FT-IR spectrum indicates the presence of different functional groups present in the biomolecules capping the nanoparticles. Further, inhibitory activity of AgNPs and leaf extract were tested against human pathogens like gram-pastive (Staphylococcus aureus, Bacillus subtilis), gram-negative (Escherichia coli and Pseudomonas aeruginosa). The results indicated that the AgNPs showed moderate inhibitory actions against human pathogens than Lagenaria siceraria leaf extract, demonstrating its antimicrobial value against pathogenic diseases

Optimization of Silver Nanoparticles Production using Aspergillus niger and Study of Antimicrobial Activity

2021 ◽  
Vol 12 (4) ◽  
pp. 2383-2388
Author(s):  
Suguna Selvakumaran ◽  
Kayathri Marimuthu ◽  
Thiruvany Poopalan ◽  
Kalaiyarasi Tamil Selvan ◽  
Nozieana Khairuddin
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Aspergillus Niger ◽  
Culture Supernatant ◽  
Gram Negative Bacteria ◽  
Mixing Ratio ◽  
Zone Of Inhibition ◽  
Gram Positive Bacteria ◽  
Physiochemical Parameters ◽  
Uv Visible

Silver nanoparticles have attracted high attention worldwide for their various applications. The physiochemical parameters such as temperature, media, mixing ratio affect the rate of synthesis of silver nanoparticles and their yield. Thus, optimization of these physiochemical parameters is needed to enhance the production of silver nanoparticles. In this study, silver nanoparticles were synthesized using Aspergillus niger culture supernatant. The produced silver nanoparticles were characterized using UV-visible Spectrophotometer at 200 nm to 700 nm, which had a peak at 450 nm, indicates the formation of silver nanoparticles. It was found that Sabouraud Dextrose Broth (SDB) as optimum media, 40 ml of supernatant and 10 ml of silver nitrate as optimum mixing ratio and 65°C as optimum temperature to produce silver nanoparticles. The optimized silver nanoparticles were subjected to antimicrobial activity, and it was found that it is highly effective towards gram-negative bacteria than gram-positive bacteria where the zone of inhibition for Escherichia coli was  7 ± 2.7 mm and 5.3 ± 2.1 mm for Staphylococcus aureus.

scholarly journals Silver-Nanoparticles Embedded Pyridine-Cholesterol Xerogels as Highly Efficient Catalysts for 4-nitrophenol Reduction

Materials ◽  
2020 ◽  
Vol 13 (7) ◽  
pp. 1486
Author(s):  
Ganesh Shimoga ◽  
Eun-Jae Shin ◽  
Sang-Youn Kim
Keyword(s):  
Silver Nanoparticles ◽  
Trisodium Citrate ◽  
Environmentally Benign ◽  
X Ray Diffraction ◽  
Physico Chemical ◽  
Efficient Reduction ◽  
Nitrophenol Reduction ◽  
Uv Visible ◽  
Average Size

Two xerogels made of 4-pyridyl cholesterol (PC) and silver-nanocomposites (SNCs) thereof have been studied for their efficient reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of aqueous sodium borohydride. Since in-situ silver doping will be effective in ethanol and acetone solvents with a PC gelator, two silver-loaded PC xerogels were prepared and successive SNCs were achieved by using an environmentally benign trisodium citrate dehydrate reducing agent. The formed PC xerogels and their SNCs were comprehensively investigated using different physico-chemical techniques, such as field emission scanning electron microscopy (FE-SEM), Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), powdered X-ray diffraction (XRD) and UV-Visible spectroscopy (UV-Vis). The FE-SEM results confirm that the shape of xerogel-covered silver nanoparticles (SNPs) are roughly spherical, with an average size in the range of 30–80 nm. Thermal degradation studies were analyzed via the sensitive graphical Broido’s method using a TGA technique. Both SNC-PC (SNC-PC-X1 and SNC-PC-X2) xerogels showed remarkable catalytic performances, with recyclable conversion efficiency of around 82% after the fourth consecutive run. The apparent rate constant (kapp) of SNC-PC-X1 and SNC-PC-X2 were found to be 6.120 × 10-3 sec-1 and 3.758 × 10-3 sec-1, respectively, at an ambient temperature.

SILVER NANOPARTICLE IMPREGNATED BIO-BASED ACTIVATED CARBON WITH ENHANCED ANTIMICROBIAL ACTIVITY

2013 ◽  
Vol 12 (04) ◽  
pp. 1350024 ◽  
Author(s):  
R. SELVAKUMAR ◽  
S. P. SURIYARAJ ◽  
V. JAYAVIGNESH ◽  
K. SWAMINATHAN
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Yeast Cells ◽  
X Ray Diffraction ◽  
Gram Positive ◽  
Biological Reduction ◽  
Gram Negative ◽  
Transmission Electron ◽  
Gram Negative Organisms ◽  
Average Size

The present study involves the production of silver nanoparticles using a novel yeast strain Saccharomyces cerevisiae BU-MBT CY-1 isolated from coconut cell sap. The biological reduction of silver nitrate by the isolate was deducted at various time intervals. The yeast cells after biological silver reduction were harvested and subjected to carbonization at 400°C for 1 h and its properties were analyzed using Fourier transform infra-red spectroscopy, X-ray diffraction, scanning electron microscope attached with energy dispersive spectroscopy and transmission electron microscopy. The average size of the silver nanoparticles present on the surface of the carbonized silver containing yeast cells (CSY) was 19 ± 9 nm. The carbonized control yeast cells (CCY) did not contain any particles on its surface. The carbonized silver nanoparticles containing yeast cells (CSY) were made into bioactive emulsion and tested for its efficacy against various pathogenic Gram positive and Gram negative bacteria. The antimicrobial activity studies indicated that CSY bioactive nanoemulsion was effective against Gram negative organisms than Gram positive organism.

scholarly journals Microwave Assisted Rapid and Green Synthesis of Silver Nanoparticles Using a Pigment Produced byStreptomyces coelicolorklmp33

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Deene Manikprabhu ◽  
K. Lingappa
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Streptomyces Coelicolor ◽  
Biological Synthesis ◽  
Toxic Substances ◽  
X Ray Diffraction ◽  
Chemical Methods ◽  
Major Drawback ◽  
Transmission Electron ◽  
Uv Visible

Traditional synthesis of silver nanoparticles using chemical methods produces toxic substances. In contrast biological synthesis is regarded as a safe and nontoxic process but the major drawback of biological synthesis is, this process is slow. In the present investigation, we developed a rapid and green synthesis of silver nanoparticles employing a pigment produced byStreptomyces coelicolorklmp33 in just 90 s. The silver nanoparticles were characterized by UV-visible spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The biobased synthesis developed in this method is a safe, rapid, and appropriate way for bulky synthesis of silver nanoparticles.

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