Uji Daya Hambat Ekstrak Etanol Batang Saluang Belum (Luvunga sarmentosa Kurz) Terhadap Bakteri  Propionibacterium acnes

Saluang Belum (Luvunga sarmentosa Kurz) adalah tumbuhan yang dikenal sebagai obat tradisional di Kalimantan Tengah. Batang L. sarmentosa Kurz dipercayai memiliki manfaat secara empiris sebagai antioksidan yang dimanfaatkan sebagai anti-aging (anti penuaan dini). Batang L. sarmentosa Kurz mengandung metabolit sekunder yaitu tanin, saponin, steroid dan flavonoid yang diduga memiliki aktivitas sebagai antibakteri. Berdasarkan khasiat empiris dan kandungan metabolit sekunder yang ada dalam tumbuhan L. sarmentosa Kurz, maka dilakukan penelitian tentang aktivitas daya hambat ekstrak etanol tumbuhan ini terhadap bakteri P. acnes yang merupakan salah satu bakteri penyebab jerawat. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas daya hambat ekstrak etanol batang L. sarmentosa Kurz terhadap bakteri P.acnes. Metode penelitian yang dilakukan pada penelitian ini dimulai dari pengambilan simplisia, pembuatan ekstrak etanol dengan metode perkolasi dan uji daya hambat dengan menggunakan metode Disk Difusion yang menggunakan kontrol positif antibiotik klindamicin. Analisis data dilakukan dengan membandingkan lebar zona hambat dari ekstrak etanol batang L. sarmentosa Kurz dengan kategori penghambatan antimikroba klindamisin berdasarkan diameter zona hambat dari Clinical and Laboratory Standards Institute. Hasil uji daya hambat didapatkan zona hambat ekstrak etanol batang Saluang Belum pada semua konsentrasi 0,5%, 1%, 5%, 10% dan 15% secara berturut-turut yaitu Intermediate, Resistant, Intermediate, Susceptible dan Susceptible dan hasil dari zona hambat klindamicin pada semua konsentrasi 0,5%,1%,5%,10%,dan 15% adalah susceptible. Simpulan dari penelitian ini adalah ekstrak etanol batang L. sarmentosa Kurz mempunyai kemampuan dalam menghambat pertumbuhan bakteri P.acnes. Kata Kunci: Obat Tradisional, Jerawat, Uji Daya Hambat, Propionibacterium acnes, Batang Saluang Belum Saluang belum (Luvunga sarmentosa Kurz) is one of traditional medicine in Central Kalimantan. The stem of L. sarmentosa Kurz is believed to have empirical benefits as an antioxidant which is used as anti-ageing (anti-ageing). The stem of L. sarmentosa Kurz contains secondary metabolites, namely tannins, saponins, steroids and flavonoids which are thought to have antibacterial activity. Based on the empirical properties and the content of secondary metabolites in L. sarmentosa Kurz, a study was conducted on the inhibitory activity of this plant's ethanol extract against P. acnes, which is one of the bacteria that causes acne. The purpose of this study was to determine the inhibitory activity of the ethanol extract of L. sarmentosa Kurz stem against P.acnes bacteria. The research method carried out in this study started from taking simplicia, making ethanol extract using the percolation method and the inhibition test using the disk diffusion method which used a positive control of the clindamycin antibiotic. Data analysis was performed by comparing the inhibition zone width of the ethanol extract of L. sarmentosa Kurz stem with the clindamycin antimicrobial inhibition category based on the inhibition zone diameter of the Clinical and Laboratory Standards Institute. The results of the inhibition test obtained the inhibition zone of Saluang Belum stem ethanol extract at all concentrations of 0.5%, 1%, 5%, 10% and 15% respectively, Intermediate, Resistant, Intermediate, Susceptible and Susceptible and the results of the inhibition zone. Clindamycin at all concentrations of 0.5%, 1%, 5%, 10%, and 15% are susceptible. This research concludes that the ethanol extract of L. sarmentosa Kurz stem can inhibit the growth of P.acnes bacteria.

Download Full-text

Uji Daya Hambat Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa blimbi L.) Terhadap Pertumbuhan Staphylococcus epidermidis

Jurnal Serambi Engineering ◽

10.32672/jse.v6i1.2609 ◽

2021 ◽

Vol 6 (1) ◽

Author(s):

Irma Zarwinda ◽

Fauziah Fauziah ◽

Shara Shevalinda ◽

Dwi Putri Rejeki

Keyword(s):

Staphylococcus Epidermidis ◽

Research Method ◽

Inhibitory Concentration ◽

Sampling Technique ◽

Ethanol Extract ◽

Inhibition Zone ◽

Chemistry Laboratory ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Training And Education

Startfruit (Averrhoa bilimbi L.) is one the kind of plant that is widely used as a traditional herbal medicineto overcome various diseases i.e. diabetes mellitus, cough, rheumatism, thrush, diabetes, toothache, acne, etc. The secondary metabolites of the starfruit leaves contain flavonoids and tannins, whereby these activecompounds can be used as an antibacterial. This study aimed to determine the inhibiting power of starfruit leaf ethanol extract against Staphylococcus epidermidis at concentrations of 25%, 50%, 75%, and 100%. The research was conducted at the AKAFARMA Laboratory and the Chemistry Laboratory , Faculty of Teacher Training and Education, Syiah Kuala University from June to July 2020. The research method was laboratory experimental using the disk diffusion method. The population of starfruit leaves was obtained from Lhokseumawe using a purposive sampling technique. Ethanol extract of starfruit leaves with concentrations of 25%, 50%, 75%, and 100%. The diameter of the inhibition zone of the ethanol extract from the starfruit leaves at concentrations of 100%, 75%, 50%, and 25% were 15 mm, 12 mm, 11 mm and 10 mm, respectively. It can be concluded that ethanol extract of starfruit leaves can inhibit the growth of Staphylococcus epidermidis with a maximum inhibitory concentration of 100%, which is classified as a strong category.

Download Full-text

Efficacy of Kumaun Himalayan Biota orientalis Endl. leaves extracts against pathogenic fungi

International Journal of Pharmacology and Toxicology ◽

10.14419/ijpt.v4i2.6702 ◽

2016 ◽

Vol 4 (2) ◽

pp. 224

Author(s):

Savita Joshi ◽

S. C. Sati ◽

Parikshit Kumar

Keyword(s):

Inhibitory Activity ◽

Ethanol Extract ◽

Pathogenic Fungi ◽

Chloroform Extract ◽

Positive Control ◽

Diffusion Method ◽

Colletotrichum Falcatum ◽

Fungal Strains ◽

Percent Inhibition ◽

Biota Orientalis

An increasing demand for natural plant products has shifted the attention from synthetic to natural antifungal agents. This study was carried out to evaluate the antifungal activity of methanol, ethanol, chloroform, hexane and water extracts of Biota orientalis Endl. leaves, a Kumaun Himalayan gymnospermic plant. The antifungal potential of all extracts of B. orientalis were tested against seven different fungal strains (Alternaria alternata, Colletotrichum falcatum, Fusarium oxysporum, Pyricularia oryzae, Sclerotinia rolfsii, Sclerotinia sclerotiorum and Tilletia indica) using agar-well diffusion method. The ethanol extract was found most active against all the pathogens tested (Percent inhibition, 27-59%) followed by hexane extract (Percent inhibition, 31-58%) and methanol extract (27-57%) while chloroform and aqueous extracts were found totally inactive against all the tested fungal strains, only chloroform extract showed inhibitory activity against S. rolfsii (% inhibition, 58%). The inhibitory activity of these extracts was found very effective as compared to Clotrimazol, standard antifungal agent that was used as positive control against tested fungal strains.

Download Full-text

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL RIMPANG KUNYIT (Curcuma longa L.) TERHADAP Staphylococcus aureus DAN Pseudomonas aeruginosa

Pharma Xplore : Jurnal Ilmiah Farmasi ◽

10.36805/farmasi.v4i1.618 ◽

2019 ◽

Vol 4 (1) ◽

pp. 278-287

Author(s):

Lia Fikayuniar ◽

Neni Sri Gunarti ◽

Mellya Apriliani

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Curcuma Longa ◽

Ethanol Extract ◽

Inhibition Zone ◽

Negative Control ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Paper Disk

ABSTRAK Kunyit (Curcuma longa L.) merupakan salah satu jenis tanaman obat yang termasuk dalam keluarga Zingiberaceae. Senyawa aktif yang terkandung dalam rimpang kunyit (Curcuma longa L.) mampu bekerja sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak etanol rimpang kunyit (Curcuma longa L.) terhadap Staphylococcus aureus dan Pseudomonas aeruginosa. Ekstraksi dilakukan dengan cara refluks menggunakan pelarut etanol 96%. Pengujian aktivitas antibakteri dilakukan menggunakan metode difusi paper disk dengan masing-masing konsentrasi ekstrak 10%, 20%, 30%, 40% b/v. Kontrol positif yang digunakan adalah Ciprofloxacin sedangkan kontrol negatif yang digunakan adalah DMSO. Hasil skrining fitokimia ekstrak etanol rimpang kunyit mengandung alkaloid, flavonoid, fenol, tanin dan terpenoid. Berdasarkan hasil penelitian, ekstrak etanol rimpang kunyit dapat menghambat bakteri Staphylococcus aureus dan Pseudomonas aeruginosa pada konsentrasi 10%, 20%, 30%, 40% dan konsentrasi 40% merupakan konsentrasi yang memberikan diameter zona hambat terbesar terhadap kedua bakteri uji yaitu 8,63 mm dan 7,8 mm. Kata Kunci : aktivitas antibakteri, Curcuma longa L., Staphylococcus aureus, ABSTRACT Turmeric (Curcuma longa L.) is one type of medicinal plant that belongs to the Zingiberaceae family. The active compounds contained in the Curcuma longa L. rhizome can work as antibacterial. This study aims to determine the antibacterial activity of the ethanol extract of Curcuma longa L. Antibacterial activity testing was carried out using the paper disk diffusion method with each extract concentration of 10%, 20%, 30%, 40%. The positive control used was Ciprofloxacin while the negative control used was DMSO. The results of phytochemical screening of the ethanol extract of Curcuma longa L. rhizome contain alkaloids, flavonoids, phenols, tannins and terpenoids. Based on the results of the study, the ethanol extract of turmeric rhizome can inhibit Staphylococcus aureus and Pseudomonas aeruginosa bacteria at concentrations of 10%, 20%, 30%, 40% and 40% concentrations which give the largest inhibition zone diameter of the two test bacteria which is 8.63 mm and 7.8 mm. Keywords: antibacterial activity, Curcuma longa L., Staphylococcus aureus,

Download Full-text

Isolasi dan identifikasi Escherichia coli dari Sumber Air Minum Kandang Broiler serta Uji Aktivitas Antibakteri Lidah Buaya

Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) ◽

10.46549/jipvet.v10i2.116 ◽

2020 ◽

Vol 10 (2) ◽

pp. 106

Author(s):

Dian Meididewi Nuraini ◽

Morsid Andityas ◽

Adi Paramarta ◽

Nur Rohman Najib ◽

Agustina Dwi Wijayanti

Keyword(s):

Escherichia Coli ◽

Methylene Blue ◽

Drinking Water ◽

Inhibitory Activity ◽

Aloe Vera ◽

Ethanol Extract ◽

Inhibition Zone ◽

Inhibition Test ◽

Aloe Barbadensis ◽

E Coli

Abstract Colibacillosis is one of the most problematic issues in the boiler industry. However, the antibiotic overuse has induced Escherichia coli resistance so that other alternative to reduce colibacillosis is needed. One of the alternatives is using aloe vera (Aloe barbadensis Miller), which has been widely used as an antibacterial agent. This study aims to isolate and identify E. coli from the broiler drinking water source and test the aloe vera antibacterial activity against it. Escherichia coli were isolated from well in three broiler farms in Moyudan District, Sleman, Yogyakarta that previously had colibacillosis. Escherichia coli were isolated using eosin methylene blue (EMB) agar and the metallic sheen colony was tested to confirm the biochemist reaction. The pure isolate of E. coli was used in the aloe vera inhibition test using Muller Hinton agar (MHA) by a Well Diffusion method. Aloe vera was processed using aquades and ethanol 70%. The aquades infusion was diluted into 12.5%, 25%, 50%, 75%, and 100% and the extract ethanol 70% was diluted into 10%, 12.5, %, 25%, 40%, and 50%. The bacterial identification showed that one of three samples contained E. coli which was then used for inhibition test. The result showed no inhibition zone in the aquades infusion while ethanol extract showed an inhibition zone in concentration 25%, 40%, and 50% of aloe vera extract with a diameter 19.5 mm, 24 mm, and 25 mm. It can be concluded that aloe vera ethanol extract has inhibitory activity against E. coli in poultry drinking water with a minimum concentration of 25%. Keywords: Aloe vera; Broiler drinking water; Escherichia coli; Inhibitory activity Abstrak Colibacilosis masih menjadi permasalahan dalam industri broiler. Penggunaan antibiotik berlebihan telah menyebabkan resistensi sehingga perlu alternatif lain. Salah satu alternatif adalah menggunakan bahan alami seperti adalah lidah buaya (Aloe barbadensis Miller) yang memilliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi E. coli dari sumber air minum di kandang broiler serta menguji aktivitas inhibisi lidah buaya terhadap bakteri tersebut. Air yang digunakan sebagai sumber E. coli berasal dari sumur di tiga peternakan broiler di Kecamatan Moyudan, Sleman, Yogyakarta yang memiliki riwayat infeksi colibacilosis. Bakteri E. coli diisolasi menggunakan media eosin methylene blue (EMB) dan diuji sifat biokimia untuk mengkonfirmasi sifat bakteri E. coli. Isolat murni E. coli digunakan pada uji daya hambat bakteri dengan metode difusi sumuran menggunakan media Muller Hinton Agar (MHA). Lidah buaya diproses menggunakan aquades dan ethanol 70%. Infusa aquades diencerkan menjadi konsentrasi 12,5%, 25%, 50%, 75%, dan 100% dan ekstrak ethanol 70% diencerkan menjadi 10%, 12., %, 25%, 40%, dan 50%. Hasil isolasi menunjukan bahwa satu sumber air dari sumur di Desa Kolowenang mengandung E. coli yang kemudian digunakan pada pengujian daya hambat. Hasil pengujian menunjukan tidak ada daya hambat yang terbentuk pada infusa aquades sedangkan ekstrak etanol lidah buaya 25%, 40%, dan 50% menunjukan adanya zona hambat sebesar 19,5 mm, 24 mm, dan 25 mm berturut-turut. Ekstrak etanol lidah buaya pada penelitian ini memiliki kemampuan menghambat pertumbuhan E. coli yang bersumber dari air minum broiler dengan konsentrasi terendah 25%. Kata kunci: Air minum broiler; Escherichia coli; Lidah buaya; Daya hambat bakteri

Download Full-text

UJI DAYA HAMBAT ANTIBAKTERI SALEP EKSTRAK ETANOL DAUN PANDAN HUTAN (Freycinetia sessiliflora Rizki.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus epidermidis

Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan ◽

10.36387/jiis.v5i2.530 ◽

2020 ◽

Vol 5 (2) ◽

pp. 376-386

Author(s):

Fitri Sri Rizki ◽

◽

Ade Ferdinan

Keyword(s):

Secondary Metabolites ◽

Staphylococcus Epidermidis ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibitory Effect ◽

Water Soluble ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Bacteria Inhibition ◽

Soluble Base

Freycinetia sessiliflora Rizki is a plant that has secondary metabolites, namely alkaloids, flavonoids, terpenoids-steroids, saponins, phenols and tannins. This study aims to determine whether there is inhibitory and concentrated ointment extract of thick pandanus leaf of the forest that can inhibit the growth of Stapylococcus epidermidis bacteria. Inhibition testing was carried out by disk diffusion method with different concentrations of 5% and 10% concentrations made 9 ointment formulas with a variety of 3 bases namely hydrocarbon base, absorbency base and water soluble base. The results of inhibitory measurements of ethanol extract ointment leaves of pandanus leaves are good or have the greatest value to the smallest inhibitory effect on the growth of Staphylococcus epidermidis bacteria with an average diameter of inhibition at F6 of 4.11 mm, F1 of 2.78 mm, F3 of 2.68 mm, F5 of 2.47 mm, F4 of 2.25 mm, F2 of 2.23 mm, F7 of 1.29, F8 of 1.13 and F9 of 0.86 mm.

Download Full-text

Antibacterial Activity of Cold Powder Preparation of (Ethanol Extract) Starfruit Leaf (Averrhoa bilimbi Linn.) Against Propionibacterium acnes

JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS ◽

10.36987/jpbn.v6i2.1677 ◽

2020 ◽

Vol 6 (2) ◽

pp. 120-128

Author(s):

Helen Anjelina Simanjuntak ◽

Kasta Gurning ◽

Verawati Br. Sinaga

Keyword(s):

Antibacterial Activity ◽

Medicinal Plant ◽

Research Method ◽

Propionibacterium Acnes ◽

Ethanol Extract ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Distilled Water ◽

Powder Preparation

Acne is a skin disease that occurs due to chronic trade that is triggered by the bacterium Propionibacterium acnes. Starfruit leaf (Averrhoa bilimbi Linn.) as a traditional medicinal plant which has antibacterial activity. The purpose of this study was to determine the antibacterial activity from ethanol extract in the preparations cold powder of starfruit leaf (Averrhoa bilimbi Linn.) againts Propionibacterium acnes. The research method was carried out experimentally, and testing for antibacterial activity was carried out using agar diffusion methods. Cold powder formulation such as F1 (5%), F2 (10%), F3 (15%), F4 (20%), positive control with clindamycin, negative control with distilled water. The results showed that the F3 (15%) and F4 (20%) formulations were more effective in inhibiting the growth of Propionibacterium acnes bacteria with inhibition zone diameters of 13.66 mm and 15.16 mm in the strong category

Download Full-text

CRUDE EXTRACTS OF MAGNOLIA ALBA FLOWERS USING N-HEXANE AND METHANOL SOLVENTS AND THEIR ANTI-ACNE POTENTIAL

Journal of medical pharmaceutical and allied sciences ◽

10.22270/jmpas.v10i6.1607 ◽

2021 ◽

Vol 10 (6) ◽

pp. 3789-3793

Author(s):

Sofia Munira

Keyword(s):

Propionibacterium Acnes ◽

Methanol Extract ◽

Antibacterial Properties ◽

Inhibition Zone ◽

Positive Control ◽

Hexane Extract ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Bacterial Inhibition

This study aimed to test the antibacterial activity of n-hexane and methanol extracts of white cempaka flowers (Magnolia alba) against Propionibacterium acnes bacteria. The extract was obtained by using the maceration method for 72 hours. Each extract was tested for its antibacterial properties using the disc diffusion method with the variant concentration of the extract used was 0.05%; 2%; 4%; 6%; and 8%. The results showed that the methanol extract had a higher inhibition zone than the n-hexane extract. In n-hexane extract, the lowest concentration of 0.05% showed an inhibition zone of 6.6 mm and the highest concentration of 8% had an inhibition zone of 9.6 mm. Whereas in methanol extract, the inhibition zone obtained was 7.3 mm at a low concentration of 0.05%, but at the highest concentration of 8% the inhibition zone obtained was 11.6 mm. The effect of extract concentration on the diameter of the inhibition zone formed is very significant (directly proportional), where the higher the concentration used, the larger the diameter of the inhibition zone produced. Antibacterial testing for both extracts showed a weak (< 10 mm) to moderate (> 10 mm) bacterial inhibition, which was similar with the inhibition zone obtained from the positive control (11-11.3 mm; moderate bacterial inhibition).

Download Full-text

SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN RACUN AYAM (Brucea javanica [L.] Merr.) ASAL KALIMANTAN SELATAN

Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan ◽

10.36387/jiis.v6i1.607 ◽

2021 ◽

Vol 6 (1) ◽

pp. 59-68

Author(s):

Sutomo Sutomo ◽

◽

Arnida Arnida ◽

Fahriah Fahriah

Keyword(s):

Antibacterial Activity ◽

Ethanol Extract ◽

Inhibition Zone ◽

Negative Control ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Inhibition Zone Diameter ◽

E Coli ◽

Zone Diameter ◽

Brucea Javanica

Racun ayam is one of the plants which has several activities in its fruits, one of which is an antibacterial. This study aims to determine the antibacterial activity of ethanol extract of racun ayam leaves. The extraction was done by maceration method using ethanol as a solvent. Antibacterial activity assay was carried out on S. aureus, E. coli, and P. aeruginosa bacteria by disk diffusion method at the concentrations of 80, 100, and 120 ppm. The results showed that the ethanol extract of B. javanica leaves had the greatest inhibition zone on E. coli with inhibition zone diameter values â€‹â€‹at 80, 100, and 120 ppm were 11.7 mm, 14.5 mm and 19.67 mm respectively. Inhibition zones in S. aureus could only be seen at 100 and 120 ppm which had inhibition zone diameters at 1.17 mm and 2.17 mm respectively, whereas in P. aeruginosa they did not have any inhibitory zone values. The results of statistical analysis showed a significance of the inhibition zone diameter values â€‹â€‹of E. coli bacteria starting at 80 ppm with a sig value of 0.034. Due to a sig value was <0.05, it was categorized as significantly different from the negative control. The bacteria of S. aureus which had â€‹â€‹<0.05 for sig values only found on 100 ppm and 120 ppm. Bacteria P. aeruginosa which had sig value >0.05 was not significantly different at all concentrations

Download Full-text

The potency of ethanolic extract from corn silk as natural antibiotics for acne-related bacteria: A preliminary study

Bangladesh Journal of Medical Science ◽

10.3329/bjms.v21i1.56331 ◽

2022 ◽

Vol 21 (1) ◽

pp. 84-89

Author(s):

Fitri A Nurani ◽

Nadia RS Rejeki ◽

Tanti Setyoputri ◽

Putri K Wardani ◽

Fatkhan B Ridwan ◽

...

Keyword(s):

Antibacterial Activity ◽

Acne Vulgaris ◽

Medical Science ◽

Ethanolic Extract ◽

Inhibition Zone ◽

Positive Control ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Traditional Medicines ◽

Corn Silk

Introduction: Corn silk or known as herb name stigma maydis is an important medicinal botanical in many traditional medicines worldwide, including jamu, an Indonesia traditional medicine. The exploration of corn silk to treat acne vulgaris is still lacking, therefore the current research was conducted to analyze the activity of ethanolic extract from corn silk (EECS) against 3 acne-related bacteria, Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus. Materials and Methods: The antibacterial activity of EECS at concentration range of 10 to 100% v/v was evaluated using the disk diffusion method. As comparation, distilled water was used as a solvent control, while 1% clindamycin was used as a positive control. Results: Shinoda’s test showed that flavonoid was detected in the EECS. The higher concentration of EECS exhibited higher diameter of inhibition zone indicating higher antibacterial activity on P. acnes, while the antibacterial activity of S. epidermidis was not increased at similar concentrations of EECS. The antibacterial activity of EECS against S. aureus decreased at the higher EECS concentration (>70%). Conclusion: Taken together, EECS is a potential as a bioactive source to inhibit the growth of acne-related bacteria P. acne, S. epidermidis and S. aureus. Further investigation is needed to explore the corn silk or stigma maydis as a medicinal botanical in jamu targeted to treat acne vulgaris. Bangladesh Journal of Medical Science Vol. 21(1) 2022 Page : 84-89

Download Full-text

Aktivitas Antibakteri Ekstrak Etanol Daun Gamal (Gliricidia sepium) pada Berbagai Konsentrasi terhadap Pertumbuhan Bakteri Streptococcus mutans secara In-Vitro

Jurnal Kesehatan ◽

10.26630/jk.v9i3.967 ◽

2018 ◽

Vol 9 (3) ◽

pp. 336

Author(s):

Ni Luh Budi Artaningsih ◽

Nur Habibah ◽

Mastra Nyoman

Keyword(s):

Antibacterial Activity ◽

Streptococcus Mutans ◽

Ethanol Extract ◽

Inhibition Zone ◽

Gliricidia Sepium ◽

Disk Diffusion ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Growth Inhibition Zone

Streptococcus mutans is a positive gram bacteria which cause dental caries. From the several previous studies, Gamal leaf has been predicted as the antibacterial agent because of their active substance such as tannins, alkaloids, saponins, and flavonoids. The objective of the study was to know the antibacterial activity of ethanol extract of Gamal leaf (Gliricidia sepium) for Streptococcus mutans in various concentrations. This study was a true experimental with post-test only control design. The determination of antibacterial activity in this study was conducted by using Kirby Bauer disk diffusion method with the various concentrations, there were 40, 50, 60, 70 and 80%. The positive and negative controls in this study were 30 µg disk diffusion of chloramphenicol and the 96% of ethanol. The average inhibition of zone diameter in the concentrations of 40, 50, 60, 70 and 80% were 11.3, 12.3, 13.4, 15.3 and 19.2 mm, respectively. One Way ANOVA statistical analysis showed that the value of p was 0.000, so there was a difference of growth inhibition zone of Streptococcus mutans in various concentrations of ethanol extract of Gamal leaf (Gliricidia sepium). Based on the result, it can be concluded that the ethanol extract of Gamal leaf (Gliricidia sepium) have strong antibacterial activity, especially to Streptococcus mutans bacteria.

Download Full-text