scholarly journals PENGARUH EKSTRAK BIJI PEPAYA (carica papaya L.) DAN EKSTRAK DAUN MIMBA (Azadirachta indica A.Juss) DAN CAMPURAN EKSTRAK BIJI PEPAYA ( carica papaya L.) DAN EKSTRAK DAUN MIMBA (Azadirachta indica A.Juss) TERHADAP DIAMETER TUBULUS SEMINIFERUS, SEL LEYDIG DAN BOBOT TESTIS MENCIT (Mus musculus)

2017 ◽  
Vol 10 (01) ◽  
Author(s):  
Apolonia M ◽  
Sukarjati Sukarjati
Keyword(s):  
Leydig Cell ◽  
Azadirachta Indica ◽  
Mus Musculus ◽  
Seminiferous Tubule ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Seed Extract ◽  
Testicular Weight ◽  
Neem Leaf Extract ◽  
Papaya Seed

Papaya (Carica papaya L. ) and Neem (Azadirachia indica A.Juss) Tree are planted as an ornamental tree and have a character as a brush plant. They vegetated in tropical and subtropical region. The seed of papaya and Neem leaf are also include of a potential plants as a antifertility. Types of bioactif compounds contained in plants, the main compounds that are derived from the steroids, alkaloids, isoflavanoid, tripernoid, and activity as xanthon antifertilities. This encourages the researcher to know the influence of ethanol seed papaya extract and neem leaf extract towards diameter of seminiferous tubule, leydig cell and the testicular weight mice (Mus musculus). The sample of this research is the mice by as much as 21 tail with a weight of 20-30 grams, aged 2.5 months. Murine in to 3 groups, each group is divided 7 treatment. As for the treatments given are extracts of flowers, leaves, and roots of hibiscus with dose control, 50, and 100 mg/kg. Granting of extracts for 35 days. On day 37 mice are in surgery for testicular weight to observations taken, the volume of the testes. The testes then made preparations and measured diameter of seminiferous tubules. This research is experimental research using Random Complete Group. The data obtained were analyzed with variant analysis (ANOVA) in one direction and continued with test LSD (Least Significant Different). The results of the research there shows the influence of the ethanol papaya seed extract, meem leaf extracy and also the micture of papaya seed extract and neem leaf extract towards the weight of mice, leydig cell and the diameter of seminiferous tubule (P < 0.05). The best result to decrease the testicular weight and diameter of seminiferous tubule was showed by the neem with the weight of 100 mg/kg BB. The best result to decrease the leydig cell was showed on the mixture of papaya seed extract and neem leaf extract in dosage of 100 mg/kg BB. This study showed that papaya seed extract, neem leaf extract and the mixture of papaya seed extract and neem leaf extract able to decrease the testicular weight, diameter of seminiferous and leydig cell. In the next day, this research supposed to be able as an antifertility in male.

scholarly journals PENGARUH PEMBERIAN EKSTRAK BIJI PEPAYA (Carica Papaya L.) DAN EKSTRAK DAUN MIMBA (Azadirachta Indica A. Juss)DAN CAMPURAN EKSTRAK BIJI PEPAYA DAN EKSTRAK DAUN MIMBA TERHADAP KUALITAS SPERMATZOA MENCIT (Mus Musculus)

WAHANA ◽  
2016 ◽  
Vol 67 (2) ◽  
pp. 59-69
Author(s):  
Waqiatul Hasanah ◽  
Dr.Dra Sukarjati M.Kes
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Seed Extract ◽  
Optimal Treatment ◽  
Neem Leaves ◽  
Papaya Seeds ◽  
Neem Leaf Extract ◽  
Papaya Seed

Seeds of papaya (Carica Papaya L.) and leaves of neem (Azadirachta indica A. Juss) is an herbal plant. In papaya seeds contain of alkaloids, Papain, tannins, saponins, Triterpenoid, Flavonoids. On the part of Neem leaves contain tannins, Saponins, Triterpenoid, Flavonoids. These compounds are compounds that function as antifertility for men. This has led the authors to conduct research on the effect of seed extract of papaya (Carica Papaya L.) and the leaf extract of neem (Azadirachta indica A. Juss) on the quality of spermatozoa of mice (Mus muscullus). The Samples were as many as 21 mice sperm tails with a weight of 20-30 grams, 2-3 months old. Mice divided in the 3 groups, each group was divided 7 treatments. The treatment given is control, papaya seed extract, neem leaf extract and a combination of both papaya seed extract and neem leaves with a concentration of 0 mg/kg bw, 50 mg/kg bw and 100 mg/kg bw 50:50 mg/kg bw, 100: 100 mg/kg bw.  35 days for extract. On day 37 mice in surgery for cauda epididymis were taken for observation of the quality of spermatozoa of mice. This study was an experimental study using randomized block design (RAK). The Observation of the quality of spermatozoa includes motility, viability, and morphology of spermatozoa concentration by using a microscope. Data were analyzed by analysis of variance (ANOVA) One Way. The results of this study showed that there is  effect of papaya seed extract and neem leaf extract and a combination of both papaya seed extract and neem leaf on motility (P <0.05), viability (P <0.05), morphology (P <0.05) and sperm concentration (P <0.05). The results of this study concluded that neem leaf extract at a concentration of 100 mg/kg bw is the optimal treatment in reducing the viability of spermatozoa of mice. Extracts combination of papaya seeds and leaves of neem with a concentration of 100 mg/kg bw is the optimal treatment in reducing the motility and concentration of spermatozoa of mice. Papaya seed extract at a concentration of 100 mg/kg bw optimal in reducing the number of normal sperm in mice. Keywords: papaya seed extract (Carica Papaya L.), Neem Leaf Extract (Azadiracta indica A. Juss), the quality of spermatozoa of mice (Mus muscullus)

scholarly journals Azadirachta indica (Neem) Leaf Extract Effect as an Option of Treatment of Ibuprofen-induced Nephrotoxicity

2020 ◽  
pp. 56-62
Author(s):  
Ifeanacho Ezeteonu Abireh ◽  
Onyinye Mary Ozioko ◽  
Ignatius Ikemefuna Ozor ◽  
Elizabeth Finbarrs- Bello ◽  
Uche Sebastine Ozioko ◽  
...  
Keyword(s):  
Serum Creatinine ◽  
Azadirachta Indica ◽  
Leaf Extract ◽  
Kidney Injury ◽  
Wistar Rat ◽  
Male Wistar Rats ◽  
Oral Ibuprofen ◽  
Group 2 ◽  
Neem Leaf Extract ◽  
Group 1

Aim: This study investigated the curative effect of the aqueous leaf extract of Azadirachta indica on Ibuprofen-induced nephrotoxicity in Wistar rat Study Design: This is an experimental research Place of Research: Department of Anatomy, College of Medicine, Enugu State University of Science and Technology. Methodology: Twenty-four male Wistar rats were divided into 6 groups, with 4 rats in each group. Group 1 was control and received oral normal saline 0.5 ml daily. Group 2-6 had induction of nephrotoxicity using oral Ibuprofen 400 mg/Kg daily for 5 days. Group 3-5 were subsequently treated with gavage Azadirachta indica leaf extract 200 mg/Kg, 400 mg/Kg and 800 mg/Kg, respectively, for 5 days. And Group 6 was treated with oral Vitamin E 1000 iu/kg for 5 days. Results: Ibuprofen induced nephrotoxicity as evidenced by elevation of serum creatinine level in group 2 (1.99 ± 0.83), when compared to 0.48 ± 0.07 obtained in group 1 (control), and Bowman’s capsule enlargement with glomerular degeneration observed in group 2. The serum creatinine levels progressively approached the level of that of the control in groups treated with Azadirachta indica leaf extract, groups 3 (1.69 ± 0.52), 4 (0.69 ± 0.10) and 5 (0.49 ± 0.10). Also, the histoarchitecture progressively normalized to that of control with each increase in dose of the extract. Conclusion: Azadirachta indica (neem) leaf extract administration led to the resolution of Ibuprofen-induced kidney injury in this study. Thus, it can serve as a treatment option for kidney injury resulting from ingestion of Ibuprofen, after the identification of the molecule responsible for this effect.

scholarly journals Protective Effect of Aqueous Crude Extract of Neem (Azadirachta indica) Leaves onPlasmodium berghei-Induced Renal Damage in Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Voravuth Somsak ◽  
Sukanya Chachiyo ◽  
Ubonwan Jaihan ◽  
Somrudee Nakinchat
Keyword(s):  
Protective Effect ◽  
Azadirachta Indica ◽  
Crude Extract ◽  
Renal Injury ◽  
Leaf Extract ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Potential Candidate ◽  
Neem Leaves ◽  
Neem Leaf Extract

Malaria is a major public health problem in the world because it can cause of death in patients. Malaria-associated renal injury is associated with 45% of mortality in adult patients hospitalized with severe form of the disease. Therefore, new plant extracts to protect against renal injury induced by malaria infection are urgently needed. In this study, we investigated the protective effect of aqueous crude extract ofAzadirachta indica(neem) leaves on renal injury induced byPlasmodium bergheiANKA infection in mice. ICR mice were injected intraperitoneally with 1 × 107parasitized erythrocytes of PbANKA, and neem extracts (500, 1,000, and 2,000 mg/kg) were given orally for 4 consecutive days. Plasma blood urea nitrogen (BUN) and creatinine levels were subsequently measured. Malaria-induced renal injury was evidenced as marked increases of BUN and creatinine levels. However, the oral administration of neem leaf extract to PbANKA infected mice for 4 days brought back BUN and creatinine levels to near normalcy, and the highest activity was observed at doses of 1,000 and 2,000 mg/kg. Additionally, no toxic effects were found in normal mice treated with this extract. Hence, neem leaf extract can be considered a potential candidate for protection against renal injury induced by malaria.

scholarly journals ANTI-TRYPANOSOMA ACTIVITY OF ETHANOLIC EXTRACT OF NEEM LEAF (Azadirachta indica) ON Trypanosoma evansi IN RATS (Rattus norvegicus)

2017 ◽  
Vol 11 (1) ◽  
pp. 27-30
Author(s):  
Yudha Fahrimal ◽  
Siti Maghfirah ◽  
Rinidar Rinidar ◽  
Al Azhar ◽  
Nuzul Asmilia ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Trypanosoma Evansi ◽  
Male Rats ◽  
Control Group ◽  
Neem Leaf Extract

The aim of this study was to determine the effect of neem leaf extract (Azadirachta indica) on parasitemia of rats infected with Trypanosoma evansi (T. evansi) Aceh local isolate. A total of 24 male rats aged three months were used in this study and randomly divided into six treatment groups equally. The negative control group (K0) without T. evansi infection and neem leaf extract, the positive control group (K1) was infected with T. evansi but no neem leaf extract given, group K2, K3, K4, and K5 were infected with 5x104 T. evansi and were given neem leaf extract after patent infection with dose of 50, 100, 400, and 800 mg/kg BW respectively. The extract was given orally for three consecutive days. On the fourth day, rat blood was drawn for parasitemia examination. The results showed that no T. evansi detected in rats in negative control group (K0), while parasitemia in group K1; K2; K3; K4; and K5 was 12,295 x106/mL; 10,495 x106/mL; 9,360 x106/mL; 5,080x106/mL; and 2,398x106/mL of blood, respectively. Percentage of inhibition of parasitemia in K2, K3, K4, and K5 reached 14.64, 23.78, 58.68, and 80.50%, respectively. Based on the result of the study, neem leaf extract of 800 mg/kg BW gave the highest reduction of parasitemia in rats infected with T. evansi.

scholarly journals Antimicrobial Activity of Azadirachta indica, against Target Pathogens and Its Utility as a Disinfectant and Floor Cleaner

2021 ◽  
Vol 10 (25) ◽  
pp. 1899-1903
Author(s):  
Mohsin Ali Khan ◽  
Shadma Yaqoob ◽  
Sharique Ahmad
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Azadirachta Indica ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Leaf Extracts ◽  
Neem Extract ◽  
Inoculum Preparation ◽  
Neem Leaf Extract ◽  
Agar Well Diffusion Method

BACKGROUND Azadirachta indica, commonly known as neem, neem tree or Indian lilac, Limbo, Nim, Nimba, Medusa and Vempu. It is typically grown in tropical and subtropical regions. Neem belongs to family meliaceae. Neem is a native tree of India. It is also called “village pharmacy of south Asia” (India) because of its enormous medicinal properties and people use it most of the time without knowing its multiple advantages. This study was done to know the efficacy of medicinal plant named Azadirachta indica (Neem) against pathogenic microorganisms and about its utility as disinfectant and floor cleaner. METHODS Agar well diffusion method, Culture plate method, inoculum preparation & its subculture methods were used to determine the antimicrobial activity of the neem leaf extract. Different concentrations of neem leaf extracts in culture plates were used for the study. In this method inhibited zones were measured. RESULTS Neem leaf extracts showed considerable antimicrobial activity against four target pathogens. In agar well diffusion method it showed maximum antimicrobial activity against Enterococcus and Staphylococcus aureus. Growth of inhibition was increased as the concentration of the neem extract in agar media increased and was maximum for Staphylococcus aureus. CONCLUSIONS The extract of A. indica, has antimicrobial activity against target pathogens Enterococcus, Staphylococcus aureus, Pseudomonas, and E.coli. With the old medicinal knowledge on neem, better economic and therapeutic utilization can be done by using modern approaches of drug development. KEY WORDS Neem Leaf extract, Antimicrobial Activity, Agar Well Diffusion Test, Zone of Inhibition

Biogenic synthesis of Silver Nanoparticles using Azadirachta indica (Neem) leaf extract targeting inhibitory action against antibiotic resistance bacteria

2021 ◽  
Vol 19 ◽  
Author(s):  
Moinuzzaman ◽  
Mohammad Abu Hena Mostofa Jamal ◽  
Helal Uddin ◽  
Shahedur Rahman ◽  
Abdul Momin ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibiotic Resistance ◽  
Azadirachta Indica ◽  
Metal Ion ◽  
Leaf Extract ◽  
Biomedical Application ◽  
Fixed Ratio ◽  
Biomedical Science ◽  
Biological Synthesis ◽  
Neem Leaf Extract

Background: In new modern science Nanotechnology is an emerging field for the researchers. Nanotechnology deals with the Nanoparticles. These Nanoparticles have a size of 1-100 nm in one dimension which are important part of biomedical science and medical chemistry, atomic physics, and all other known fields. Nanoparticles are used in broad range due to its small size, orientation, physical properties. Objectives: This study was designed to synthesized Silver nanoparticles using Azadirachta indica (Neem) leaf extract and evaluate biomedical application. Methods: Aqueous extract of Azadirachta indica is used for the synthesis of AgNPs. 1ml of the extract was added to (30- 60) ml of 1mM solution of silver nitrate drop by drop. Silver nanoparticle was characterized by UV-Visible Spectroscopy, Fourier Transform Infrared Spectroscopy, Dynamic Light Scattering Spectroscopy, X-ray Diffraction, Transmission Electron Microscopy, Antibacterial activity studies. Results: The Biological synthesis of Silver nanoparticles (AgNPs) was done by using the aqueous solution of Azadirachta indica leaf extract and AgNO3. A fixed ratio of plant extract to metal ion was used to prepare AgNPs and the formation of the nanoparticles was observed by the color change. The nanoparticles were characterized by UV-vis Spectrophotometer, FTIR, DLS, XRD and TEM. The nanoparticles were found have the size ranges from 30-60 nm. The biosynthesized silver nanoparticles had bactericidal effect against antibiotic resistance pathogenic microorganisms such as Bascillus subtilis, pseudomonas aeruginosa and Bascillus cereus. Conclusion: Silver nanoparticles were synthesized by Azadirachta indica leaf extract can be used as a therapeutic candidate for biomedical applications.

scholarly journals The effect of aqueous seed extract of neem (Azadirachta indica A. Juss) on liver histology of male mice (Mus musculus albinus)

2018 ◽  
Author(s):  
Elsa Lisanti ◽  
Dondin Sajuthi ◽  
Muhammad Agil ◽  
R. Iis Arifiantini ◽  
Adi Winarto
Keyword(s):  
Azadirachta Indica ◽  
Mus Musculus ◽  
Seed Extract ◽  
Liver Histology ◽  
Male Mice
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