scholarly journals PENGARUH PEMBERIAN EKSTRAK BIJI PEPAYA (Carica Papaya L.) DAN EKSTRAK DAUN MIMBA (Azadirachta Indica A. Juss)DAN CAMPURAN EKSTRAK BIJI PEPAYA DAN EKSTRAK DAUN MIMBA TERHADAP KUALITAS SPERMATZOA MENCIT (Mus Musculus)

WAHANA ◽  
2016 ◽  
Vol 67 (2) ◽  
pp. 59-69
Author(s):  
Waqiatul Hasanah ◽  
Dr.Dra Sukarjati M.Kes
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Seed Extract ◽  
Optimal Treatment ◽  
Neem Leaves ◽  
Papaya Seeds ◽  
Neem Leaf Extract ◽  
Papaya Seed

Seeds of papaya (Carica Papaya L.) and leaves of neem (Azadirachta indica A. Juss) is an herbal plant. In papaya seeds contain of alkaloids, Papain, tannins, saponins, Triterpenoid, Flavonoids. On the part of Neem leaves contain tannins, Saponins, Triterpenoid, Flavonoids. These compounds are compounds that function as antifertility for men. This has led the authors to conduct research on the effect of seed extract of papaya (Carica Papaya L.) and the leaf extract of neem (Azadirachta indica A. Juss) on the quality of spermatozoa of mice (Mus muscullus). The Samples were as many as 21 mice sperm tails with a weight of 20-30 grams, 2-3 months old. Mice divided in the 3 groups, each group was divided 7 treatments. The treatment given is control, papaya seed extract, neem leaf extract and a combination of both papaya seed extract and neem leaves with a concentration of 0 mg/kg bw, 50 mg/kg bw and 100 mg/kg bw 50:50 mg/kg bw, 100: 100 mg/kg bw.  35 days for extract. On day 37 mice in surgery for cauda epididymis were taken for observation of the quality of spermatozoa of mice. This study was an experimental study using randomized block design (RAK). The Observation of the quality of spermatozoa includes motility, viability, and morphology of spermatozoa concentration by using a microscope. Data were analyzed by analysis of variance (ANOVA) One Way. The results of this study showed that there is  effect of papaya seed extract and neem leaf extract and a combination of both papaya seed extract and neem leaf on motility (P <0.05), viability (P <0.05), morphology (P <0.05) and sperm concentration (P <0.05). The results of this study concluded that neem leaf extract at a concentration of 100 mg/kg bw is the optimal treatment in reducing the viability of spermatozoa of mice. Extracts combination of papaya seeds and leaves of neem with a concentration of 100 mg/kg bw is the optimal treatment in reducing the motility and concentration of spermatozoa of mice. Papaya seed extract at a concentration of 100 mg/kg bw optimal in reducing the number of normal sperm in mice. Keywords: papaya seed extract (Carica Papaya L.), Neem Leaf Extract (Azadiracta indica A. Juss), the quality of spermatozoa of mice (Mus muscullus)

scholarly journals PENGARUH EKSTRAK BIJI PEPAYA (carica papaya L.) DAN EKSTRAK DAUN MIMBA (Azadirachta indica A.Juss) DAN CAMPURAN EKSTRAK BIJI PEPAYA ( carica papaya L.) DAN EKSTRAK DAUN MIMBA (Azadirachta indica A.Juss) TERHADAP DIAMETER TUBULUS SEMINIFERUS, SEL LEYDIG DAN BOBOT TESTIS MENCIT (Mus musculus)

2017 ◽  
Vol 10 (01) ◽  
Author(s):  
Apolonia M ◽  
Sukarjati Sukarjati
Keyword(s):  
Leydig Cell ◽  
Azadirachta Indica ◽  
Mus Musculus ◽  
Seminiferous Tubule ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Seed Extract ◽  
Testicular Weight ◽  
Neem Leaf Extract ◽  
Papaya Seed

Papaya (Carica papaya L. ) and Neem (Azadirachia indica A.Juss) Tree are planted as an ornamental tree and have a character as a brush plant. They vegetated in tropical and subtropical region. The seed of papaya and Neem leaf are also include of a potential plants as a antifertility. Types of bioactif compounds contained in plants, the main compounds that are derived from the steroids, alkaloids, isoflavanoid, tripernoid, and activity as xanthon antifertilities. This encourages the researcher to know the influence of ethanol seed papaya extract and neem leaf extract towards diameter of seminiferous tubule, leydig cell and the testicular weight mice (Mus musculus). The sample of this research is the mice by as much as 21 tail with a weight of 20-30 grams, aged 2.5 months. Murine in to 3 groups, each group is divided 7 treatment. As for the treatments given are extracts of flowers, leaves, and roots of hibiscus with dose control, 50, and 100 mg/kg. Granting of extracts for 35 days. On day 37 mice are in surgery for testicular weight to observations taken, the volume of the testes. The testes then made preparations and measured diameter of seminiferous tubules. This research is experimental research using Random Complete Group. The data obtained were analyzed with variant analysis (ANOVA) in one direction and continued with test LSD (Least Significant Different). The results of the research there shows the influence of the ethanol papaya seed extract, meem leaf extracy and also the micture of papaya seed extract and neem leaf extract towards the weight of mice, leydig cell and the diameter of seminiferous tubule (P < 0.05). The best result to decrease the testicular weight and diameter of seminiferous tubule was showed by the neem with the weight of 100 mg/kg BB. The best result to decrease the leydig cell was showed on the mixture of papaya seed extract and neem leaf extract in dosage of 100 mg/kg BB. This study showed that papaya seed extract, neem leaf extract and the mixture of papaya seed extract and neem leaf extract able to decrease the testicular weight, diameter of seminiferous and leydig cell. In the next day, this research supposed to be able as an antifertility in male.

scholarly journals Protective Effect of Aqueous Crude Extract of Neem (Azadirachta indica) Leaves onPlasmodium berghei-Induced Renal Damage in Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Voravuth Somsak ◽  
Sukanya Chachiyo ◽  
Ubonwan Jaihan ◽  
Somrudee Nakinchat
Keyword(s):  
Protective Effect ◽  
Azadirachta Indica ◽  
Crude Extract ◽  
Renal Injury ◽  
Leaf Extract ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Potential Candidate ◽  
Neem Leaves ◽  
Neem Leaf Extract

Malaria is a major public health problem in the world because it can cause of death in patients. Malaria-associated renal injury is associated with 45% of mortality in adult patients hospitalized with severe form of the disease. Therefore, new plant extracts to protect against renal injury induced by malaria infection are urgently needed. In this study, we investigated the protective effect of aqueous crude extract ofAzadirachta indica(neem) leaves on renal injury induced byPlasmodium bergheiANKA infection in mice. ICR mice were injected intraperitoneally with 1 × 107parasitized erythrocytes of PbANKA, and neem extracts (500, 1,000, and 2,000 mg/kg) were given orally for 4 consecutive days. Plasma blood urea nitrogen (BUN) and creatinine levels were subsequently measured. Malaria-induced renal injury was evidenced as marked increases of BUN and creatinine levels. However, the oral administration of neem leaf extract to PbANKA infected mice for 4 days brought back BUN and creatinine levels to near normalcy, and the highest activity was observed at doses of 1,000 and 2,000 mg/kg. Additionally, no toxic effects were found in normal mice treated with this extract. Hence, neem leaf extract can be considered a potential candidate for protection against renal injury induced by malaria.

scholarly journals Perbandingan kualitas spermatozoa tikus wistar (rattus norvegicus) yang diberi ekstrak buah pare (momordica charantia L) dengan ekstrak biji pepaya (carica papaya L)

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Kyrie E. Wenur ◽  
Lusiana Satiawati ◽  
Lydia Tendean
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Carica Papaya ◽  
Seed Extract ◽  
Momordica Charantia ◽  
Bitter Melon ◽  
Fruit Extract ◽  
Treatment Groups ◽  
Papaya Seeds

Abstract: Male participation in contraception is still lacking. Pare (Momordica charantia L) is known having an inhibitory effect spermatogenesis and seeds of papaya (Carica papaya L) contain the alkaloid compounds that are anti-fertility, both can be used as a male contraceptive. The purpose of this study was to determine sperm quality comparison Wistar rats (Rattus norvegicus) were given a fruit extract of pare (Momordica charantia L) with the seed extract of papaya (Carica papaya L). The research used a completely randomized experimental design. There are nine samples of Wistar rats (Rattus norvegicus) males were divided into three groups and one group consisting three Wistar rats. Over the past 50 days, extracts of pare (Momordica charantia L) is given to the group 1 at 70mg / kg/day, while in the group 2 given seed extract of papaya (Carica papaya L) of 70mg / kg/day and the control group was given no treatment. The results showed significant differences in sperm motility among the treatment groups 1 with extract of bitter melon fruit (Momordica charantia L) and 2 treatment groups with the extract of papaya seeds (Carica papaya L), namely a decrease in sperm motility (p = 0.002). Conclusion, the fruit extract of bitter melon (Momordica charantia L) and seed extract of papaya (Carica papaya L) affects the quality of spermatozoa.Keywords: pare, papaya seeds, quality of spermatozoa. Abstrak: Partisipasi pria dalam menggunakan alat kontrasepsi masih sangat kurang. Pare (Momordica charantia L) diketahui memiliki efek penghambatan spermatogenesis dan biji pepaya (Carica papaya L) mengandung senyawa alkaloid yang bersifat antifertilitas, keduanya dapat digunakan sebagai bahan kontrasepsi pria. Tujuan penelitian ini adalah mengetahui perbandingan kualitas spermatozoa tikus wistar (Rattus norvegicus) yang diberi ekstrak buah pare (Momordica charantia L) dengan ekstrak biji pepaya (Carica papaya L). Penelitian ini menggunakan metode penelitian eksperimental dengan menggunakan rancangan acak lengkap. Sampel yang digunakan adalah sembilan tikus wistar (Rattus norvegicus) jantan yang dibagi menjadi tiga kelompok dan satu kelompok terdiri dari tiga ekor tikus wistar. Selama 50 hari, ekstrak buah pare (Momordica charantia L) diberikan pada kelompok perlakuan 1 sebesar 70mg/kgBB per hari, sedangkan pada kelompok perlakuan 2 diberikan ekstrak biji pepaya (Carica papaya L) sebesar 70mg/kgBB per dan kelompok kontrol tidak diberikan perlakuan. Hasil penelitian menunjukan adanya perbedaan signifikan pada motilitas spermatozoa antara kelompok perlakuan 1 dengan pemberian ekstrak buah pare (Momordica charantia L) dan kelompok perlakuan 2 dengan pemberian ekstrak biji pepaya (Carica papaya L) yaitu penurunan motilitas spermatozoa ( p = 0,002).Kesimpulan, ekstrak buah pare (Momordica charantia L) dan ekstrak biji pepaya (Carica papaya L) berpengaruh terhadap kualitas spermatozoa. Kata kunci: buah pare, biji pepaya, kualitas spermatozoa

scholarly journals CHEMICAL CHARACTERISTIC AND ANTIOXIDANT ACTIVITY OF METHANOL EXTRACT NEEM LEAVES (AZADIRACHTA INDICA JUSS)

AGROINTEK ◽  
2021 ◽  
Vol 15 (2) ◽  
pp. 649-657
Author(s):  
Supriyanto Supriyanto ◽  
Simon Bambang Widjanarko ◽  
Muhaimin Rifa'i ◽  
Yunianta Yunianta
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Azadirachta Indica ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Chemical Characteristic ◽  
Neem Leaves ◽  
Ic50 Values ◽  
Neem Leaf Extract

The neem plant (Azadirachta indica) is a herb with the potential as a source of antioxidants. This study aimed to identify compounds that exist in the neem leaf and determine their antioxidant activity. Neem leaf extract was collected by using 80% methanol.  Furthermore, methanol extracts from neem leaves with the highest antioxidant activity were partitioned with n-hexane, ethyl acetate, and water. This research were analyzed by partition for antioxidant activity, and DPPH (1,1-diphenyl-2-pycrilhydrazil) and the half-maximal inhibitory concentration (IC50) values were determined. The results showed that the ethyl acetate partition had the highest antioxidant activity, with IC50 values of 1.004 µg/ml. Neem leaf extract has the potential for a use as an herbal medicine in the treatment of various diseases..

scholarly journals Efek Pemberian Ekstrak Etanol Daun Mimba (Azadirachta indica A. Juss) terhadap Struktur Histologis Pankreas Tikus Putih (Rattus norvegicus L.) Hiperglikemia

2021 ◽  
Vol 6 (2) ◽  
pp. 115-123
Author(s):  
Nunuk Shofiati ◽  
Siti Muflichatun Mardiati ◽  
Agung Janika Sitasiwi ◽  
Sri Isdadiyanto
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Histological Structure ◽  
Distilled Water ◽  
Treatment Groups ◽  
Randomized Design ◽  
Neem Leaves ◽  
Neem Leaf Extract

Indikator klinis penyakit Diabetes Melitus adalah hiperglikemia. Mimba (Azadirachta indica A. Juss) merupakan salah satu tumbuhan yang berpotensi sebagai alternatif obat herbal hiperglikemia. Penelitian ini bertujuan untuk menganalisis pengaruh ekstrak etanol daun mimba terhadap struktur histologis pankreas pada tikus hiperglikemia. Penelitian ini mengunakan Rancangan Acak Lengkap (RAL) dengan jumlah tikus 24 ekor yang dibagi menjadi 6 kelompok perlakuan dan 4 kali ulangan. P0 (kontrol normal) adalah kelompok tikus normal yang diberi akuades, P1 (kontrol negatif) adalah tikus hiperglikemia yang diberi akuades. P2 (kontrol positif) adalah kelompok tikus hiperglikemia yang diberi glibenklamid dosis 2,25 mg/kg BB. P3, P4, dan P5 adalah kelompok tikus yang diberi ekstrak etanol daun mimba dosis 100, 200, dan 400 mg/kg BB. Data dianalisis dengan ANOVA pada signifikansi 95%. Hasil penelitian menunjukkan bahwa perlakuan ekstrak etanol daun mimba dosis 100, 200, dan 400 mg/kg BB tidak memberikan pengaruh nyata terhadap bobot pankreas, diameter, luas, dan densitas pulau Langerhans (P>0,05). Skoring struktur pulau Langerhans berdasarkan uji Mann-Whitneymenunjukkan hasil beda nyata pada kelompok  tikus yang diberi daun mimba (P≤0,05). Kesimpulan dari penelitian ini, pemberian ekstrak daun mimba dosis 400mg/kg BB menunjukkan adanya perbaikan morfologi pulau Langerhans. The clinical indicator of Diabetes mellitus was hyperglycemia. Azadirachta indica A. Juss was a plant has the potential to alternative medicine for hyperglycemia. The study was to analyze the ethanol neem leaf extract effect on histological structure of hyperglycemic rat pancreas. This study used a completely randomized design (CRD) with 24 rats were divided into 6 treatment groups and 4 replications. P0 (control) was a normal rats group were given distilled water, P1 (negative control) was a hyperglycemic rats group were given distilled water. P2 (positive control) was a hyperglycemic rats were given 2.25 mg/kg BW of glibenclamide. P3, P4, and P5 were rats were given 100, 200, and 400 mg/kg BW of ethanolic neem leaf extract.The data analyzed by ANOVA at 95% significance showed the treatment of 100, 200, and 400 mg/kg BW ethanolic neem leaf extract had no significant effect on the pancreatic weight, diameter, area, and density of Langerhans islet (P> 0.05). The score of Langerhans islet structure based on the Mann-Whitney test showed significant differences in the groups of mice given neem leaves (P≤0.05). Treatment of 400mg/Kg BW neem leaf extract showed an improvement in the morphology of the islets of Langerhans. 

scholarly journals Anealisis Uji Pendahuluan Aktivitas Antikanker Ekstrak Biji Pepaya (Carica papaya L) dengan Metode BSLT

2019 ◽  
Vol 4 (1) ◽  
pp. 7-10
Author(s):  
Ninik Mas Ulfa ◽  
Galuh Gondo Kusumo ◽  
Ilil Maidatuz Zulfa
Keyword(s):  
Anticancer Activity ◽  
Carica Papaya ◽  
Tropical Plant ◽  
Preliminary Study ◽  
Papaya Seeds ◽  
Preliminary Research ◽  
Papaya Seed

ABSTRAKTumbuhan pepaya (carica papaya L) merupakan tumbuhan tropis yang banyak terdapat di Indonesia. Tumbuhan ini mempunyai banyak manfaatnya mulai dari buah, biji, hingga daunnya. Penelitian pendahuluan menyebutkan buah pepaya mengandung alkaloid dan flavonoid yang berkhasiat sebagai antikanker. Senyawa BenzylIsothiocyanat diketahui banyak terdapat pada biji dan buah pepaya yang sudah matang. Kandungan BenzylIsothiocyanat mempunyai khasiat sebagai antikanker. Pemanfaatan limbah biji pepaya pada penelitian ini untuk membuktikan aktivitas Benzyl-Isothiacyanat yang berkhasiat sebagai antikanker. Penelitian ini merupakan penelitian pendahuluan untuk menganalisis aktivitas antikanker dari Ekstrak kental biji pepaya dengan menggunakan metode BSLT. Konsentrasi ekstrak kentak yang digunakan yaitu 100 ppm, 200 ppm dan 300 ppm masing-masing diujikan pada 10 larva udang dalam air laut. Diperoleh hasil rata-rata kematian pada konsentrasi 100 ppm adalah 4,3, 200 ppm adalah 5,3 dan 300 ppm adalah 6,7. Hasil regresi linearitas menunjukkan aktivitas antikaker pada uji BSLT dari Ekstrak kental biji pepaya dengan LC50 sebesar 163,89 ppm. Dengan demikian ekstrak kental biji pepaya tersebut berpotensi untuk dikembangkan sebagai bahan antikanker alamiKata kunci: Aktivitas antikanker, Carica papaya, metode BSLT.ABSTRACTPapaya plant (carica papaya L) is a tropical plant that is widely found in Indonesian. This plant has many benefits ranging from fruit, seeds, to leaves. Preliminary research says papaya fruit contains alkaloids andflavonoids which are efficacious as anticancer. Benzyl-Isothiocyanat compounds are known to be widely found in ripe papaya seeds and fruit. The content of Benzyl-Isothiocyanat has properties as an anticancer. The use of papaya seed waste in this study is to prove the activity of Benzyl-Isothiacyanat which is efficacious as an anticancer. This research is a preliminary study to analyze the anticancer activity of thick papaya seeds using the BSLT method. The concentrations of used fart extracts were 100 ppm, 200 ppm and 300 ppm each tested on 10 shrimp larvae in seawater. The results of the average mortality at concentrations of 100 ppm were 4.3, 200 ppm were 5.3 and 300 ppm was 6.7. The linearity regression results showed the anticaker activity in the BSLT test from the thick extract of papaya seeds with LC50 of 163.89 ppm. Thus the thick extract of papaya seeds has the potential to be developed as a natural anticancer material.Key Words : Anticancer activity, BSLT method, Carica papaya.

scholarly journals Azadirachta indica (Neem) Leaf Extract Effect as an Option of Treatment of Ibuprofen-induced Nephrotoxicity

2020 ◽  
pp. 56-62
Author(s):  
Ifeanacho Ezeteonu Abireh ◽  
Onyinye Mary Ozioko ◽  
Ignatius Ikemefuna Ozor ◽  
Elizabeth Finbarrs- Bello ◽  
Uche Sebastine Ozioko ◽  
...  
Keyword(s):  
Serum Creatinine ◽  
Azadirachta Indica ◽  
Leaf Extract ◽  
Kidney Injury ◽  
Wistar Rat ◽  
Male Wistar Rats ◽  
Oral Ibuprofen ◽  
Group 2 ◽  
Neem Leaf Extract ◽  
Group 1

Aim: This study investigated the curative effect of the aqueous leaf extract of Azadirachta indica on Ibuprofen-induced nephrotoxicity in Wistar rat Study Design: This is an experimental research Place of Research: Department of Anatomy, College of Medicine, Enugu State University of Science and Technology. Methodology: Twenty-four male Wistar rats were divided into 6 groups, with 4 rats in each group. Group 1 was control and received oral normal saline 0.5 ml daily. Group 2-6 had induction of nephrotoxicity using oral Ibuprofen 400 mg/Kg daily for 5 days. Group 3-5 were subsequently treated with gavage Azadirachta indica leaf extract 200 mg/Kg, 400 mg/Kg and 800 mg/Kg, respectively, for 5 days. And Group 6 was treated with oral Vitamin E 1000 iu/kg for 5 days. Results: Ibuprofen induced nephrotoxicity as evidenced by elevation of serum creatinine level in group 2 (1.99 ± 0.83), when compared to 0.48 ± 0.07 obtained in group 1 (control), and Bowman’s capsule enlargement with glomerular degeneration observed in group 2. The serum creatinine levels progressively approached the level of that of the control in groups treated with Azadirachta indica leaf extract, groups 3 (1.69 ± 0.52), 4 (0.69 ± 0.10) and 5 (0.49 ± 0.10). Also, the histoarchitecture progressively normalized to that of control with each increase in dose of the extract. Conclusion: Azadirachta indica (neem) leaf extract administration led to the resolution of Ibuprofen-induced kidney injury in this study. Thus, it can serve as a treatment option for kidney injury resulting from ingestion of Ibuprofen, after the identification of the molecule responsible for this effect.

scholarly journals Effect of neem leaf and banana pulp extracts on shelf life and quality of mango (Mangifera indica L.)

2018 ◽  
Vol 16 (3) ◽  
pp. 343-350 ◽  
Author(s):  
Rabeya Akter Sarmin ◽  
Shamim Ahmed Kamal Uddin Khan ◽  
Kanij Fatema ◽  
Sabiha Sultana
Keyword(s):  
Shelf Life ◽  
Disease Severity ◽  
Leaf Extract ◽  
Disease Incidence ◽  
Mangifera Indica ◽  
Soluble Solids ◽  
Postharvest Quality ◽  
Ambient Conditions ◽  
Neem Leaf Extract

The study was carried out to minimize the postharvest loses and extend shelf life of mango fruitby maintaining physico-chemical properties. The variety selected for the study was “Amrapali”. Freshly harvested mango was treated with different concentrations (20% and 40%) of neem leaf and banana pulp extract alone or in combination. Untreated mango was considered as control. All treated and untreated mango was kept into paper cartons at room condition. The treated fruits showed significant differences in case of total soluble solids content, titratable acidity, vitamin C, disease incidence, disease severity and shelf life in comparison to control fruits. Among the treatments, T2 (neem leaf extract at 20%) and T5 (neem leaf extract 40% + banana pulp extract 40%) treatments showed longer shelf life (9.92 and 10.25 days, respectively), slower changes in color (score 2.77 and 2.93, respectively) and firmness (score 2.67 and 2.77, respectively); less disease severity (score 2.93 and 3.57, respectively), disease incidence (46.67% and 60.00%) and lower loss in weight (38.04% and 35.17%, respectively) at 9 DAT (Days after treatment). On the other hand, total soluble solid was highest in T3 (neem leaf extract 40%) treated fruitswith18.73% more Brix at 13 DAT in comparison to control and other treatments. The effectiveness of the treatment T5 (neem leaf extract 40% + banana pulp extract 40%) was meaningful which could be recommended for maintenance of postharvest quality of mango stored in ambient conditions. J. Bangladesh Agril. Univ. 16(3): 343–350, December 2018

scholarly journals Viability of Carica papaya L. Seeds by the Tetrazolium Test

2018 ◽  
Vol 10 (2) ◽  
pp. 335 ◽  
Author(s):  
Sara M. C. Carvalho ◽  
Salvador B. Torres ◽  
Erivanessa C. Sousa ◽  
Danielle M. M. Sousa ◽  
Kleane T. O. Pereira ◽  
...  
Keyword(s):  
Decision Making ◽  
Carica Papaya ◽  
Seed Viability ◽  
Germination Test ◽  
Long Period ◽  
Physiological Quality ◽  
Rapid Tests ◽  
Papaya Seeds ◽  
Tetrazolium Test

Germination test for papaya seeds demands thirty days to obtain the results, which is a long period for decision-making. Thus, rapid tests become essential tools to evaluate seed viability, and the tetrazolium test is an alternative for this purpose. Hence, this study aimed to establish the adequate procedure to apply this test, in order to reduce the time of evaluation of papaya seeds. For that, four tetrazolium solution concentrations (0.05, 0.1, 0.5 and 1%) and three staining times (3, 6 and 9 hours) were tested at temperatures of 35 and 40 ºC. The experimental design was completely randomized in 4 × 3 + 1 factorial scheme (concentrations × staining times + one control = germination test). It was found that the tetrazolium test allows to evaluate the physiological quality of papaya seeds, thus reducing the time for decision-making. For the test, we recommend using the concentrations of 0.1% for 9 hours or 1% for 6 hours of staining, at temperature of 40 ºC.

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