Chromatographic analysis and physicochemical evaluation of the essential oil of Bauhinia monandra Kurz flowers

The study of chromatographic techniques, classical and modern, describes the simplicity and, at the same time, the advances that this area has undergone in recent years in quality scientific research and also in learning in undergraduate and postgraduate courses around the world. This paper investigate a characterization by thin layer chromatography (TLC) and gas chromatography coupled with mass spectrometry (GC-MS), as a method developed by graduate students that involve a combination of a classic and modern technique, as well as results about the physicochemical properties of the essential oil of Bauhinia monandra flower. Essential oil was extracted by Clevenger, the TLC was performed in different eluents and developers, and thus the retention factors (Rfs), and the chemical profile by GC-MS were obtained. The essential oil of the flowers showed a yield of 0.06%, positive solubility in ethanol 70%, refractive index of 1.3621, optical rotation of +36.4αD and relative density of 0.941 g mL-1 at 20 °C. In the TLC analysis 18 Rfs were observed after the use of different developers, with the predominant class of oxygenates compounds. In the GC-MS analysis, 7 compounds were observed, being two majorities, characterized as panaxene with 20.51% and the α-guaiene with 33.39%. The essential oil of B. monandra flower showed a predominance of 70.22% of sesquiterpenic compounds. The allied techniques, classic and modern, demonstrated different ways of evaluating the essential oil through its chemical composition, both techniques showed high efficiency and precision, in addition was an appropriate project developed by postgraduate students.

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PHYTOCHEMICAL INVESTIGATION OF TERMINALIA BELLIRICA FRUIT INSIDE

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i18.34347 ◽

2019 ◽

pp. 191-194 ◽

Cited By ~ 1

Author(s):

HAZRA K

Keyword(s):

Essential Oil ◽

Traditional Healing ◽

Phytochemical Screening ◽

Traditional Medicines ◽

Phytochemical Investigation ◽

Physicochemical Evaluation ◽

Terminalia Bellirica ◽

Layer Chromatography ◽

Gallic Acid Equivalent ◽

Inner Parts

Objective: The fruit of Terminalia bellirica Roxb. or Baheda (Family Combretaceae) is an extensively used herb in traditional medicines. The fruit as a whole or a part of it is used in traditional healing. Fruit has two major parts, namely, pericarp and seed, pericarp, in turn, consists of epicarp, mesocarp, and endocarp. Ayurvedic compound formulations such as Triphala and Pathyadi contain pericarp of the fruit. The present study is aiming a comparative phytochemical investigation of fruit parts such as epicarp, mesocarp, and seed along with the whole fruit. Methods: The study included physicochemical evaluation, phytochemical screening, biochemical assay, and thin-layer chromatography profile of the inner parts of the fruit. Results: The study shows that seeds are rich in essential oil (13.25%), but very poor quantity of phenolics (0.65 gallic acid equivalent [GAE]) and flavonoids (0.77 quercetin equivalent [QE]) is present compared to epicarp (139.05 GAE and 141.26 QE) and mesocarp (135.23 GAE and 142.05 QE). In addition to that, qualitative phytochemical screening revealed that seeds are void of steroids and lignans. Conclusion: The study concluded that seeds are less important in respect to the presence of secondary metabolites. It may also be concluded that mesocarp and epicarp are holding major responsibilities of therapeutic values imposed on the fruit.

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Chemical profile and antioxidant, antibacterial, and cytotoxic activities on Artemia salina from the essential oil of leaves and xylopodium of Cochlospermum regium

Scientific Electronic Archives ◽

10.36560/15120221506 ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

Roberta Heloísa de Paula Magalhães ◽

Antonio Carlos Pereira de Menezes Filho ◽

Matheus Vinicius Abadia Ventura ◽

Hellen Regina Fernandes Batista-Ventura ◽

Carlos Frederico de Souza Castro ◽

...

Keyword(s):

Antioxidant Activity ◽

Refractive Index ◽

Essential Oil ◽

Relative Density ◽

Optical Rotation ◽

Artemia Salina ◽

Cytotoxic Activities ◽

Chemical Profile ◽

Radical Reduction ◽

Antibacterial Potential

Cochlospermum regium is a shrub plant species from the Cerrado domain used in traditional medicine. This study aimed to evaluate the chemical profile and antioxidant, antibacterial and cytotoxic activities on Artemia salina from the essential oil of fresh leaves and xylopodium. Fresch leaves and xylopodium of C. regium were collected in an area of Cerrado domain in Goiás State, Brazil, 2021. The essential oil was obtained by hydrodistillation, the yield was quantified and the chemical profile determined by gas chromatography with mass spectrometry (GC-MS). Physicochemical analyzes were carried out for organoleptic analysis (color and appearance), solubility, relative density (g mL-1), refractive index, optical rotation (αD), antioxidant activity in DPPH radical reduction (IC50 µL mL-1), antibacterial activity on Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Salmonella serovar Enteritidis and Salmonella serovar Thyphymurium by the disc method (mm), and cytotoxicity bioassay on Artemia salina (LC50 µg mL-1). The major compounds for the essential oil of fresh leaves were viridiflorol 10.21%, Copaen-4-α-ol <β>, longiborneol 9.07 and β-bisabolene 11.48%, and for the essential oil of xylopodium β-selinene 26.17%, aromadendrene 8.66 % and thujopsene 8.09%. The yield was 0.58 and 0.33%, color slightly yellow and yellow for fresh leaves and xylopodium, respectively. Positive solubility, refractive index of 1.3468 and 1.3347, optical rotation +48.8 and +21.5, relative density 0.932 and 0.936 g mL-1, antioxidant activity IC50 = 47.65 and 111.16 µL mL-1 for fresh leaves essential oil and xylopodium, respectively. The essential oil from fresh leaves showed high antibacterial potential for all strains, as well as for cytotoxic activity on A. salina with LC50 = 90.17 and 625.08 µg mL-1, respectively.

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Caracterização química e atividades antioxidante e antifúngica do óleo essencial das flores de [Cochlospermum regium (Mart. ex Schrank.) Pilger] (Bixaceae)

Revista Principia - Divulgação Científica e Tecnológica do IFPB ◽

10.18265/1517-0306a2020v1n52p80-91 ◽

2020 ◽

Vol 1 (52) ◽

pp. 80

Author(s):

Antonio Carlos Pereira de Menezes Filho ◽

Wendel Cruvinel De Sousa ◽

Carlos Frederico De Souza Castro

Keyword(s):

Antioxidant Activity ◽

Refractive Index ◽

Antifungal Activity ◽

Essential Oil ◽

High Efficiency ◽

Optical Rotation ◽

Antioxidant Agent ◽

Agricultural Use ◽

Liquid Yield ◽

Organoleptic Parameters

<p class="Normal1">The objective of this work was to characterize the chemical and physicochemical profile, and the antioxidant and antifungal activities of the essential oil from Cochlospermum regium flowers. The flowers were collected in a riparian Cerrado area. The essential oil was extracted by hidrodestilation in Clevenger. The organoleptic parameters were evalueted for (color, visual and aroma), for physicochemical, yield (%), solubility, optical rotation (αD ), refractive index (20 °C) and density (25 °C g mL-1), antioxidant activity were determined by the reduction of free radical DPPH, and the antifungal activity was determined through the percentage of growth inhibition on Sclerotinia sclerotiorum, Colletotrichum gloeosporioides and Aspergillus flavus. The essential oil from C. regium flowers had an oily, clear, crystalline and strongly aromatic liquid, yield of 0.099%, positive solubility, optical rotation +36.1 αD , refractive index 1.3297 20 °C, relative density of 0.933 g mL-1. The chemical profile showed the following major compounds ocimene<(E)-β-> with 15.87%, caryophyllene E 11.53%, γ-muurolene 20.07%, bicyclogermacrene 16.11% and, rosifoliol with 31.09%. The antioxidant activity showed high efficiency in reducing of the DPPH radical in all concentrations varied between 50 to 0.031 mg mL -1 between 95.25% to 40.09%. The antifungal activity showed 100% of mycelial inhibition in 100 and 50 µL mL-1 concentrations for S. sclerotinia, C. gloeosporioides and A. flavus. In the lowest concentration of 1.56 µL mL-1 inhibition was also observed for S. sclerotiorum and A. flavus and for 3.13 µL mL-1 for C. gloeosporioides with 26.12; 6.08 and 10.07%, respectively. The essential oil of the flowers from C. regium proved to be an efficient natural product for the development of medicines, as an antioxidant agent and, antifungal for agricultural use.</p>

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Detection of Antibacterial and Antioxidant Compounds in the Essential Oil of Schizonepeta annua (Pall.) Schischk. Using High-Performance Thin-Layer Chromatography–Direct Bioautography and Gas Chromatography–Quadrupole Time-of-Flight Mass Spectrometry

JPC - Journal of Planar Chromatography - Modern TLC ◽

10.1556/1006.2019.32.5.2 ◽

2019 ◽

Vol 32 (5) ◽

pp. 359-364 ◽

Cited By ~ 2

Author(s):

Zehui Li ◽

Xi Bai ◽

Qingling Ma ◽

Haji Akber Aisa ◽

Maitinuer Maiwulanjiang

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Essential Oil ◽

Thin Layer ◽

Thin Layer Chromatography ◽

High Performance ◽

Antioxidant Compounds ◽

Flight Mass Spectrometry ◽

Direct Bioautography ◽

Layer Chromatography

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Essential Oil of Deverra tortuosa Aerial Parts: Detailed Chemical Profile, Allelopathic, Antimicrobial, and Antioxidant Activities

Chemistry & Biodiversity ◽

10.1002/cbdv.202000914 ◽

2021 ◽

Vol 18 (4) ◽

Author(s):

Eman M. Fayed ◽

Ahmed M. Abd‐EIGawad ◽

Abdelsamed I. Elshamy ◽

El‐Sayed F. El‐Halawany ◽

Yasser A. EI‐Amier

Keyword(s):

Essential Oil ◽

Antioxidant Activities ◽

Chemical Profile ◽

Aerial Parts ◽

Detailed Chemical

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Innovation in a Continuous System of Distillation by Steam to Obtain Essential Oil from Persian Lime Juice (Citrus latifolia Tanaka)

Molecules ◽

10.3390/molecules26144172 ◽

2021 ◽

Vol 26 (14) ◽

pp. 4172

Author(s):

José Daniel Padilla-de la Rosa ◽

Magaly Dyanira Manzano-Alfaro ◽

Jaime Rosalío Gómez-Huerta ◽

Enrique Arriola-Guevara ◽

Guadalupe Guatemala-Morales ◽

...

Keyword(s):

Essential Oil ◽

Oil Recovery ◽

Steam Distillation ◽

Optical Rotation ◽

Process Time ◽

Chemical Compositions ◽

Distillation Process ◽

Steam Consumption ◽

Lime Juice ◽

Oil Fractions

The citrus industry is one of the most important economic areas within the global agricultural sector. Persian lime is commonly used to produce lime juice and essential oil, which are usually obtained by batch distillation. The aim of this work was to validate a patented continuous steam distillation process and to both physically and chemically characterize the volatile fractions of essential Persian lime oil. Prior to distillation, lime juice was obtained by pressing the lime fruit. Afterwards, the juice was subjected to a continuous steam distillation process by varying the ratio of distillate flow to feed flow (0.2, 0.4, and 0.6). The distillate oil fractions were characterized by measuring their density, optical rotation, and refractive index. Gas chromatography GC-FID was used to analyze the chemical compositions of the oil fractions. The process of continuous steam distillation presented high oil recovery efficiencies (up to 90%) and lower steam consumption compared to traditional batch process distillation since steam consumption ranged from 32 to 60% for different steam levels. Moreover, a reduction in process time was observed (from 8 to 4 h). The oil fractions obtained via continuous steam distillation differed significantly in their composition from the parent compounds and the fractions.

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Prevalence of diterpenes in essential oil of Euphorbia mauritanica L.: Detailed chemical profile, antioxidant, cytotoxic and phytotoxic activities

Chemistry & Biodiversity ◽

10.1002/cbdv.202100238 ◽

2021 ◽

Author(s):

Abdelsamed Elshamy ◽

Ahmed F. Essa ◽

Seham S. El-Hawary ◽

Ahmed M. Abd El Gawad ◽

Tahia M. Kubacy ◽

...

Keyword(s):

Essential Oil ◽

Chemical Profile ◽

Detailed Chemical

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TLC analysis of some phenolic compounds in kombucha beverage

Acta periodica technologica ◽

10.2298/apt0435199m ◽

2004 ◽

pp. 199-205 ◽

Cited By ~ 3

Author(s):

Radomir Malbasa ◽

Eva Loncar ◽

Ljiljana Kolarov

Keyword(s):

Phenolic Compounds ◽

Thin Layer ◽

Thin Layer Chromatography ◽

Green Tea ◽

Black Tea ◽

Wide Range ◽

Tea Fungus ◽

Natural Phenolic Compounds ◽

Tlc Analysis ◽

Layer Chromatography

Black and green tea contains a wide range of natural phenolic compounds Flavanoids and their glycosides, catechins and the products of their condensation, and phenolic acids are the most important. Kombucha beverage is obtained by fermentation of tea fungus on black or green tea sweetened with sucrose. The aim of this paper was to investigate the composition of some phenolic compounds, catechin, epicatechin, quercetin, myricetin, gallic and tanic acid, and monitoring of their status during tea fungus fermentation. The method used for this study was thin layer chromatography with two different systems. The main phenolic compounds in the samples with green tea were catechin and epicatechin, and in the samples with black tea it was quercetin.

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THE STRUCTURE OF RETAMINE AND THE PARTIAL SYNTHESIS OF THE (−)-ENANTIOMORPH

Canadian Journal of Chemistry ◽

10.1139/v65-270 ◽

1965 ◽

Vol 43 (7) ◽

pp. 2012-2016 ◽

Cited By ~ 3

Author(s):

Kju Hi Shin ◽

L. Fonzes ◽

Léo Marion

Keyword(s):

Nuclear Magnetic Resonance ◽

Magnetic Resonance ◽

Thin Layer ◽

Thin Layer Chromatography ◽

Opposite Sign ◽

Optical Rotation ◽

Partial Synthesis ◽

Dehydration Product ◽

Layer Chromatography ◽

Nuclear Magnetic

Previous work by many authors has led to the assumption that retamine might be (+)-12-hydroxysparteine. A partial synthesis of the enantiomorph of this compound has been effected by dehydration of (+)-13-hydroxylupanine and hydroboration of the product. The dehydration product consisted of two components that were separated by thin-layer chromatography and identified by the characteristics of their nuclear magnetic resonance (n.m.r.) spectra as Δ12,13-and Δ13,14-dehydrolupanine. Hydroboration of the Δ12,13-isomer gave rise to (−)-12-hydroxy-sparteine having, in thin-layer chromatography, the same Rf value as natural retamine and the same optical rotation numerically, although of opposite sign. The synthetic base had the same infrared and n.m.r. spectra as the alkaloid and the two had superimposable Debye–Scherrer patterns. Evidence is given showing the hydroxyl to be equatorial.

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