scholarly journals AKTIVITAS ANTIMIKROBA MINYAK ESENSIAL JAHE MERAH (Zingiber officinale var. Rubrum) DAN LENGKUAS MERAH (Alpinia purpurata K. Schum) TERHADAP BAKTERI PATOGEN DAN PERUSAK PANGAN

2015 ◽  
Vol 35 (01) ◽  
pp. 43 ◽  
Author(s):  
Tita Rialita ◽  
Winiati Pudji Rahayu ◽  
Lilis Nuraida ◽  
Budi Nurtama
Keyword(s):  
Antimicrobial Activity ◽  
Refractive Index ◽  
Essential Oil ◽  
Essential Oils ◽  
Optical Rotation ◽  
Acid Number ◽  
Food Spoilage ◽  
E Coli ◽  
Spoilage Bacteria ◽  
Red Ginger

The aims of this study was to determine the characteristics, composition and antimicrobial activity of essential oils of local Indonesian red ginger and red galangal against four pathogenic and food spoilage bacteria, which were B.cereus ATCC 10876, E. coli ATCC 25922, S. typhimuriumATCC 14028, and P. aeruginosa ATCC 27853. Analysis of physicochemical characteristics was carried outin accordance with ISO7355:1985. The chemical compositionwas analyzed using aGC-MS. The antimicrobial activity was determined by disc diffusion method and broth microdillution method was used for determine MIC and MBC values. Red ginger essential oil characteristic was brownish yellow, specific gravity 0.883, refractive index 1.480, optical rotation -8.45o, clear soluble (1:1) in 90 % alcohol, 2.06 acid number and 42.45 ester number. Redgalangal essential oil had a characteristic bright yellow color, specific gravity 0.895, refractive index 1.496, optical rotation -9.15o, clear soluble (1:1) in 90 % alcohol, 1.95 acid number and 140.15 ester number. The major component of red ginger essential oils were trimethyl-heptadien-ol, ar-curcumene, camphene, carbaldehyde, -sesquiphellandrene, and nerol; while the major component of red galangal essential oil were 1.8-cineole, chavicol, 9-desoxo-9-xi-hydroxy-3-pentaacetate-3,5,7,8,9,12-Ingol,- caryophyllene and -selinene. The essential oil of red ginger and red galangal hadmoderate antibacterial activity against pathogenic and food spoilage bacteria with the average inhibition zone 7.17-10.33 and 7.25-11.17mm.Red ginger essential oils could inhibit the growth of tested bacteria with MIC values of 2.65-3.97 mg/mL and MBC value of 3.10-5.29 mg/mL, while the red galangal essential oil could inhibit the growth of tested bacteria with MIC values of 1.79-4.03 mg mL and MBC values of 1.79-4.92 mg/mL. Based on the MIC and MBC values,all tested bacteriasensitivity to essential oils of red ginger and galangal red decline in a row B.cereus > E. coli > S. typhimurium> P. aeruginosa. Sensitivity of Gram positive and Gram negative bacteria to both essential oils demonstrate the potential of the oils to be used as a natural preservative in the food industry.Keywords: Antimicrobial, essential oil, red ginger, red galangal ABSTRAKPenelitian ini bertujuan untuk mengetahui karakteristik, komposisi dan aktivitas antimikroba minyak esensial jahe merah dan lengkuas merah lokal Indonesia terhadap empat spesies bakteri patogen dan perusak pangan, yaitu B.cereus ATCC 10876, E.coli ATCC 25922, S. typhimurium ATCC 14028, dan P. aeruginosa ATCC 27853. Analisis karakteristikfisika-kimia dilakukan sesuai standar ISO 7355:1985. Komposisi kimia dianalisis menggunakan alat GC-MS. Pengujian aktivitas antimikroba dilakukan dengan metode difusi cakram untuk menentukan zona hambat, sertabroth microdillution untuk menentukan nilai Minimum Inhibitory Concentration (MIC) dan Minimum Bactericidal Concentration (MBC).Karakteristik minyak esensial jahe merah yang dihasilkan yaitu kuning kecoklatan, berat jenis 0,883, indeks bias 1,480, putaran optik -8.45, larut jernih (1:1) dalam alkohol 90%, bilangan asam 2,06, dan bilangan ester 42,45. Minyak esensial lengkuas merah memiliki karakteristik warna kuning terang, berat jenis 0,895, indeks bias 1,496, putaran optik-9.15, larut jernih (1:1) dalam alkohol 90%, bilangan asam 1,95 dan bilangan ester 140,15. Komponen mayor minyak esensial jahe merah terdiri dari trimethyl-heptadien-ol, ar-curcumene, camphene, carbaldehyde, -sesquiphellandrene, dan nerol; sedangkan komponen mayor minyak esensial lengkuas merah terdiri dari 1.8-cineole, chavicol,9-desoxo9-xi-hydroxy-3,5,7,8,9,12-pentaacetat-ingol, -caryophyllenedan -selinene. Minyak esensial jahe merah dan lengkuas merah memiliki aktivitas antibakteri yang bersifat moderat terhadap bakteri patogen dan perusak pangan, dengan kisaran zona hambat rata-rata 7,17-10,33 mm dan 7,25-11,17 mm. Minyak esensial jahe merah dapat menghambatpertumbuhan bakteri uji pada nilai MIC 2,65-3,97 mg/mL dan nilai MBC 3,10-5,29 mg/mL, sedangkan minyak esensial lengkuas merah dapat menghambat bakteri uji dengan nilai MIC 1,79-4,03 mg/mL dan nilai MBC 1,79-4,92 mg/mL. Berdasarkan nilai MIC dan MBC, sensitivitas bakteri uji terhadap minyak esensial jahe merah dan lengkuas merah menurun berturut-turut dari B. cereus > E. coli > S. typhimurium > P. aeruginosa. Sensitivitas bakteri Gram positif dan Gram negatif terhadap kedua minyak esensial ini menunjukkan potensi minyak esensial jahe merah dan lengkuas merah untuk digunakan sebagai pengawet alami di industri pangan.Kata kunci: Antimikroba, jahe merah, lengkuas merah, minyak esensial

scholarly journals Comparison of the Chemical Composition and Antimicrobial Activity of Thymus serpyllum Essential Oils

2015 ◽  
Vol 43 (2) ◽  
pp. 432-438 ◽  
Author(s):  
Aneta WESOŁOWSKA ◽  
Monika GRZESZCZUK ◽  
Dorota JADCZAK ◽  
Paweł NAWROTEK ◽  
Magdalena STRUK
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Micrococcus Luteus ◽  
Gas Chromatography Mass Spectrometry ◽  
E Coli ◽  
Thymus Serpyllum ◽  
Main Components ◽  
Total Oil

The chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of Thymus serpyllum and Thymus serpyllum‘Aureus’ has been investigated by gas chromatography-mass spectrometry (GC-MS). Forty-seven compounds (99.67% of the total oil) wereidentified in the essential oil of T. serpyllum. The main components found in the oil were carvacrol (37.49%), -terpinene (10.79%), -caryophyllene (6.51%), p-cymene (6.06%), (E)--ocimene (4.63%) and -bisabolene (4.51%). Similarly, carvacrol (44.93%), -terpinene(10.08%), p-cymene (7.39%) and -caryophyllene (6.77%) dominated in the oil of T. serpyllum ‘Aureus’. A total of forty three compounds wereidentified in this oil, representing 99.49% of the total oil content. On the basis of the obtained data it was proved that the content of 1-octen-3-ol,eucalyptol, (Z)--ocimene, (E)--ocimene, -terpinene, carvacrol methyl ether, germacrene D and -bisabolene was significantly higher for T.serpyllum while T. serpyllum ‘Aureus’ was characterized by a significantly higher content of 3-octanone, 3-octanol, p-cymene, borneol andcarvacrol. The isolated essential oils were evaluated for their antimicrobial activity against nine reference strains (Escherichia coli, Staphylococcusaureus, Staphylococcus epidermidis, Streptococcus agalactiae, Enterococcus faecalis, Bacillus cereus, Micrococcus luteus, Proteus vulgaris and Candidaalbicans) by the microdilution technique. Based on this test, the minimum inhibitory concentrations (MIC) of essential oil were calculated. Thevolatile oil obtained from T. serpyllum showed the highest antimicrobial activity relative to the strain of E. coli (MIC=0.025 μL/mL) and to theyeast C. albicans (MIC=0.05 μL/mL). Similarly, a significant antimicrobial activity exhibited T. serpyllum ‘Aureus’ essential oil, although the MICvalues obtained in that case for E. coli and C. albicans strains were twice as high and were respectively 0.05 μL/mL and 0.1 μL/mL.

scholarly journals Monitoring of Antimicrobial Activity of Essential Oils Using Molecular Markers

2009 ◽  
Vol 3 (1) ◽  
pp. 103-107 ◽  
Author(s):  
Hend A. Hamedo
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antimicrobial Agents ◽  
Random Amplified Polymorphic Dna ◽  
Antimicrobial Effect ◽  
E Coli ◽  
Specific Effects ◽  
And Staphylococcus Aureus ◽  
Rosemary Essential Oil

Technological application of essential oils, as natural antimicrobial agents, to reduce the effect of pathogenic microorganisms, requires new methods of detection. The present work evaluated the parameters of antimicrobial activity of the essential oils of rosemary (Rosmarinus officinalis) on two pathogenic strains Escherichia coli and Staphylococcus aureus. The MBC and MIC values were of 2.5, 25 μl ml-1, and values of 1.25 and 5 μl ml-1 for the two strains respectively. In this study, an attempt has been made to evaluate randomly amplified polymorphic DNA (RAPD) analysis for its potential to establish antimicrobial effect of rosemary essential oil. For the preliminary assessment, this study compared the effects occurring at molecular levels in E. coli and Staph. aureus exposed to rosemary essential oil at the MIC concentrations for the two organisms. The qualitative modifications arising in random amplified polymorphic DNA (RAPD) profiles as a measure of DNA effects were compared with control which showed many differences. In conclusion, the measurement of parameters at molecular levels is valuable for investigating the specific effects of agents interacting with DNA.

scholarly journals Efficiency of Basil Essential Oil Antimicrobial Agents under Different Shading Treatments and Harvest Times

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1574
Author(s):  
Zoran S. Ilić ◽  
Lidija Milenković ◽  
Ljubomir Šunić ◽  
Nadica Tmušić ◽  
Jasna Mastilović ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antimicrobial Agents ◽  
Antimicrobial Activities ◽  
Plant Production ◽  
Exposure Control ◽  
Harvest Time ◽  
E Coli ◽  
Growing Conditions

The aim of this study was to determine the antimicrobial activity of essential oils obtained from sweet basil (Ocimum basilicum L. cv. ‘Genovese’) cultivated in the open field under different shading conditions (red, blue, and pearl nets with a shade index of 50% and full sunlight exposure (control plants)), harvested at different times. The antimicrobial activity of basil essential oils (BEOs) obtained from all samples was determined for four microorganisms, while determinations for an additional five microorganisms included samples from non-shaded plants, plants grown under red and pearl nets, and second harvest of plants grown under blue net. Basil essential oil exhibited antimicrobial activity surpassing the activity of relevant commercial antibiotics regardless of growing conditions in the case of B. cereus, K. pneumoniae and C. albicans, while superior antimicrobial activity was exhibited in the case of essential oils from plants grown under blue nets in the case of S. aureus, E. coli and P. vulgaris. The influence of the application of colored shading nets was highly significant (p < 0.01) in the cases of all analyzed microorganisms except C. albicans and P. aeruginosa, while the influence of harvest time was proven in the cases of all microorganisms except K. pneumoniae. ANOVA proved that antimicrobial activities are highly dependent on the methods of plant production, shading treatment, and harvest time. Obtained results are discussed in relation to previously determined composition and yield of essential oils from basil grown under shade nets and harvested in different periods.

scholarly journals EFEK AROMATERAPI MINYAK ATSIRI MAWAR (Rosa damascena MILL.) DAN KULIT JERUK LIMAU (Citrus amblycarpa) TERHADAP JUMLAH MIKROBA UDARA RUANGAN BERPENDINGIN

2020 ◽  
Vol 19 (2) ◽  
Author(s):  
Oom Komala ◽  
Novi Fajar Utami ◽  
Siti Mariyam Rosdiana
Keyword(s):  
Refractive Index ◽  
Essential Oil ◽  
Optical Rotation ◽  
Acid Number ◽  
National Standard ◽  
Plate Count ◽  
Rosa Damascena ◽  
Specific Level ◽  
Energy Material ◽  
Plate Count Method

Energy, material, and or other components were entered to the air by activities of human could cause air quality down to a specific level that could affect human health. The bacteria in the human oral cavity such as Staphylococcus sp. were found in the air during common activities such as coughing, sneezing, and speaking. The research aimed to assess the influence of the combination of oils essential of Rosa damacena and leather oil of Citrus amblycarpa against a decline in the total of microbes on air-conditioned rooms. The method of the research  was used the evaporation of aromatherapy and plate count method. The results were showed that P3 with the concentration of oils essential of R. damacena 2% and leather oil of C. amblycarpa 5% most effective could decrease the total of microbes with an average of 72.5%. Organoleptic, relative density, refractive index, solubility in 90% ethanol, and acid number analysis of Rosa damacena and Citrus amblycarpa leather essential oils met the Indonesian National Standard requirements except for the acid number of leatherlime essential oil and the optical rotation of rose essential oil. It is concluded that the combination of oils essential R. damacena and leather oil of C. amblycarpa  could reduce the total of microbes on air-conditioned rooms.

scholarly journals Comparative antimicrobial activity of clove and fennel essential oils against food borne pathogenic fungi and food spoilage bacteria

2012 ◽  
Vol 11 (94) ◽  
pp. 16065-16070 ◽  
Author(s):  
Javed Shabnam ◽  
Mushtaq Sobia ◽  
Khokhar Ibatsam ◽  
Ahmad Rauf ◽  
Saleem Haider M
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Pathogenic Fungi ◽  
Food Spoilage ◽  
Food Borne ◽  
Spoilage Bacteria

scholarly journals Antimicrobial Activity of Selected Essential Oils against Selected Pathogenic Bacteria: In Vitro Study

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 546
Author(s):  
Nikola Puvača ◽  
Jovana Milenković ◽  
Tamara Galonja Galonja Coghill ◽  
Vojislava Bursić ◽  
Aleksandra Petrović ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Tea Tree ◽  
E Coli

The worldwide problem of infectious diseases has appeared in recent years, and antimicrobial agents are crucial in reducing disease emergence. Nevertheless, the development and distribution of multidrug-resistant (MDR) strains in pathogenic bacteria, such as Escherichia coli, Staphylococcus aureus, Salmonella Typhi and Citrobacter koseri, has become a major society health hazard. Essential oils could serve as a promising tool as a natural drug in fighting the problem with these bacteria. The current study aimed to investigate the antimicrobial effectiveness of tea tree (Melaleuca alternifolia (Maiden and Betche) Cheel), rosemary (Rosmarinus officinalis L.), eucalyptus (Eucalyptus obliqua L’Hér.), and lavender (Lavandula angustifolia Mill) essential oils. The antimicrobial properties of essential oils were screened against four pathogenic bacteria, E. coli, S. aureus, S. Tyhpi, and C. koseri, and two reference bacterial strains, while for the testing, the agar well diffusion method was used. Gas chromatography (GC) and gas chromatography–mass spectrometric (GC–MSD) analyses were performed on essential oils. The obtained results showed that M. alternifolia essential oil is the richest in terpinen-4-ol, R. officinalis and E. oblique essential oils in 1,8-cineole, and L. angustifolia essential oil in α-terpinyl acetate. In addition, the main bioactive compounds present in the essential oil of tea tree are rich in α-pinene (18.38%), limonene (7.55%) and γ-terpinene (14.01%). The essential oil of rosemary is rich in α-pinene (8.38%) and limonene (11.86%); eucalyptus essential oil has significant concentrations of α-pinene (12.60%), p-cymene (3.24%), limonene (3.87%), and γ-terpinene (7.37%), while the essential oil of lavender is rich in linalool (10.71%), linalool acetate (9.60%), α-terpinyl acetate (10.93%), and carbitol (13.05%) bioactive compounds, respectively. The obtained results from the in vitro study revealed that most of the essential oils exhibited antimicrobial properties. Among the tested essential oils, tea tree was discovered to demonstrate the strongest antimicrobial activity. The recorded MIC of S. Typhi was 6.2 mg/mL, 3.4 mg/mL of C. koseri, 3.1 mg/mL of E. coli, and 2.7 mg/mL of E. Coli ATCC 25922, compared to M. alternifolia. Similarly, only S. aureus ATCC 25923 showed antimicrobial activity towards R. officinalis (1.4 mg/mL), E. oblique (2.9 mg/mL), and L. angustifolia (2.1 mg/mL). Based on the obtained results, it is possible to conclude that tea tree essential oil might be used as an ecological antimicrobial in treating infectious diseases caused by the tested pathogens.

scholarly journals Quality Assessment of the Essential Oil from Eucalyptus globulus Labill of Blida (Algeria) Origin

2014 ◽  
Vol 36 ◽  
pp. 303-315 ◽  
Author(s):  
Mohamed Nadjib Boukhatem ◽  
Mohamed Amine Ferhat ◽  
Abdelkrim Kameli ◽  
Fairouz Saidi ◽  
Kerkadi Walid ◽  
...  
Keyword(s):  
Refractive Index ◽  
Essential Oil ◽  
Specific Gravity ◽  
Eucalyptus Globulus ◽  
Optical Rotation ◽  
Chemical Constituents ◽  
Chemical Properties ◽  
Acid Number ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components

Despite the reputation earned by aromatic and medicinal plants of Algeria, the chemical constituents of Eucalyptus globulus essential oil (EGEO) of Blida origin has not previously been investigated. Thus, the present study has been conducted for the determination of chemical constituents and different physico-chemical properties of the EGEO. Chemical composition of the EGEO, grown in Algeria, was analysed by Gas Chromatography-Mass Spectrometry. The chemical components were identified on the basis of Retention Time and comparing with mass spectral database of standard compounds. Relative amounts of detected compounds were calculated on the basis of GC peak areas. Fresh leaves of E. globulus on steam distillation yielded 0.96 % (v/w) of essential oil whereas the analysis resulted in the identification of a total of 11 constituents, 1.8 cineole (85.8 %), α-pinene (7.2 %), and β-myrcene (1.5 %) being the main components. Other notable compounds identified in the oil were β-pinene, limonene, α-phellandrene, γ-terpinene, linalool, pinocarveol, terpinen-4-ol, and α-terpineol. The physical properties such as specific gravity, refractive index and optical rotation and the chemical properties such as saponification value, acid number and iodine number of the EGEO were examined. The oil extracted has been analyzed to have 1.4602 - 1.4623 refractive index value, 0.918 - 0.919 specific gravity (sp.gr.), +9 - +10 optical rotation that satisfy the standards stipulated by European Pharmacopeia. All the physical and chemical parameters were in the range indicated by the ISO standards. Our findings will help to access the quality of the Eucalyptus oil which is important in the production of high value essential oils that will help to improve the economic condition of the community as well as the nation.

Pelatihan Pengolahan Jahe Menjadi Minyak Atsiri Dengan Teknik Penyulingan Pada Kelompok Petani Desa Junrejo Kecamatan Junrejo Kota Batu

2018 ◽  
Vol 2 (1) ◽  
pp. 13
Author(s):  
Nurul Muddarisna ◽  
Yekti Sri Rahayu ◽  
Mohamad Su’i
Keyword(s):  
Refractive Index ◽  
Essential Oil ◽  
Specific Gravity ◽  
Essential Oils ◽  
Oil Content ◽  
Acid Value ◽  
Training Phase ◽  
Ginger Rhizome ◽  
Long Time ◽  
Red Ginger

Ginger farmers in the Junrejo village, Junrejo sub district, Batu,  generally sell ginger in fresh form which hasa risk of low profit.  Storage of fresh ginger for a long time can reduce the content of essential oils resulting in decreasedquality and selling value.  One of techniques that can be used to minimize the risk is to process the fresh ginger into theessential oils. The purpose of this program was to provide counseling about post-harvest handling of ginger and trainingof fresh ginger processing into essential oils with distillation techniques. The target of this program was to improve skillsof  ginger farmers to distillate fresh ginger rhizome into essential oil. During the training, farmers showed enthusiasmand high spirits in following the training phase. The results of calculation of essential oil content of two types of freshginger used in the training was 0.15% in emprit ginger and 0.2% in red ginger. The results of laboratory analysis ofessential oil characteristic of ginger emprit  showed that the emprit ginger produced by Junrejo village farmers consisted of acid value, specific gravity (at 250C) and refractive index (at 250C) of 0.98% ; 1.498; and 0.98, respectively

scholarly journals Chemical composition and antimicrobial activity of Pinus halepensis from Algeria

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
Messaoud Ramdani ◽  
RIMA HAICHOUR1 ◽  
TAKIA LOGRADA ◽  
PIERRE CHALARD ◽  
GILLES FIGUEREDO4
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Pinus Halepensis ◽  
Antibacterial Activities ◽  
Klebsiella Pneumonia ◽  
E Coli ◽  
Aerial Parts ◽  
Methyl Butanoate

Abstract. Haichour R, Lograda T, Ramdani M, Chalard P, Figueredo G. 2020. Chemical composition and antimicrobial activity of Pinus halepensis from Algeria. Biodiversitas 21: 4345-4360. The chemical composition of Pinus halepensis essential oils and their antibacterial activities were investigated. Plant samples were collected in the flowering stage from the East locality of Algeria. The aerial parts of P. halepensis obtained from fifteen localities were subjected to a hydro-distillation, providing pale yellow viscous oils. The obtained yield average was 0.64 ± 0.37%. The analysis of the chemical composition of essential oils was performed by using GC and GC / MS. A total of thirty-seven compounds representing 99.4 ± 0.5% of the total oils were identified in P. halepensis. The hydrocarbon compounds were found to dominate essential oils in P. halepensis. In addition, the major compounds were caryophyllene-E (29.06 ± 8.5%), myrcene (19.14 ± 6.67%), a-pinene (16.86 ± 3.35%), phenyl ethyl 3-methyl butanoate (5.67 ± 2.47%), a-humulene (4.81 ± 1.43%), terpinolene (3.94 ± 1.6%) and sabinene (3.11 ± 1.84%). Based on their chemical composition, essential oil samples were clustered in five groups by UPGMA analysis. The first group includes the Tafrent essential oil population. It is characterized by the phenyl ethyl-3-methyl butanoate – Sabinene ‒ Δ3-carene chemotype. The second group was subdivided into two clusters. The first cluster or chemotype was characterized by the myrcene ‒ α-pinene ‒ caryophyllene-E. The second chemotype was characterized by caryophyllene-E ‒ α-humulene ‒ myrcene. The essential oil of P. halepensis is an effective antimicrobial against Staphylococcus aureus; Klebsiella pneumonia; Salmonella enteric spp arizonae; Listeria innocua; Proteus mirabilis and Bacillus cereus, and no effect on the E. coli and Pseudomonas aeruginosa.

scholarly journals Antimicrobial effect of essential oil isolated from Eucalyptus globulus Labill. from Montenegro

2011 ◽  
Vol 29 (No. 3) ◽  
pp. 277-284 ◽  
Author(s):  
B. Damjanović-Vratnica ◽  
T. Đakov ◽  
D. Šuković ◽  
J. Damjanović
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Eucalyptus Globulus ◽  
Food Poisoning ◽  
Antimicrobial Effect ◽  
Gas Chromatography Mass Spectrometry ◽  
E Coli ◽  
Alpha Pinene ◽  
Spoilage Bacteria ◽  
Main Components

Chemical composition of the essential oil of Eucalyptus globulus Labill., grown in Montenegro, was analysed by gas chromatography-mass spectrometry and its antimicrobial activity was evaluated against 17 microorganisms, including food poisoning and spoilage bacteria and human pathogens.The Eucalyptus essential oil yield was 1.8% (w/w) on the fresh weight basis, whereas the analysis resulted in the identification of a total of 11 constituents, 1.8 cineole (85.8%), &alpha;-pinene (7.2%), and &beta;-myrcene (1.5%) being the main components. Other compounds identified in the oil were<br />&beta;-pinene, limonene, &alpha;-phellandrene, &gamma;-terpinene, linalool, pinocarveol, terpinen-4-ol, and &alpha;-terpineol. The results of the antimicrobial activity tests revealed that the essential oil of E. globulus has rather a strong antimicrobial activity, especially against Streptococcus pyogenes, Escherichia coli, Candida albicans, Staphylococcus aureus, Acinetobacter baumannii, and Klebsiella pneumoniae. Minimum inhibitory concentration revealed the lowest activity against Pseudomonas aeruginosa and Salmonela infantis (3.13 mg/ml) while the highest activity was against S. aureus, E. coli, and S. pyogenes (0.09 mg/ml).

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