scholarly journals EFEK AROMATERAPI MINYAK ATSIRI MAWAR (Rosa damascena MILL.) DAN KULIT JERUK LIMAU (Citrus amblycarpa) TERHADAP JUMLAH MIKROBA UDARA RUANGAN BERPENDINGIN

2020 ◽  
Vol 19 (2) ◽  
Author(s):  
Oom Komala ◽  
Novi Fajar Utami ◽  
Siti Mariyam Rosdiana
Keyword(s):  
Refractive Index ◽  
Essential Oil ◽  
Optical Rotation ◽  
Acid Number ◽  
National Standard ◽  
Plate Count ◽  
Rosa Damascena ◽  
Specific Level ◽  
Energy Material ◽  
Plate Count Method

Energy, material, and or other components were entered to the air by activities of human could cause air quality down to a specific level that could affect human health. The bacteria in the human oral cavity such as Staphylococcus sp. were found in the air during common activities such as coughing, sneezing, and speaking. The research aimed to assess the influence of the combination of oils essential of Rosa damacena and leather oil of Citrus amblycarpa against a decline in the total of microbes on air-conditioned rooms. The method of the research  was used the evaporation of aromatherapy and plate count method. The results were showed that P3 with the concentration of oils essential of R. damacena 2% and leather oil of C. amblycarpa 5% most effective could decrease the total of microbes with an average of 72.5%. Organoleptic, relative density, refractive index, solubility in 90% ethanol, and acid number analysis of Rosa damacena and Citrus amblycarpa leather essential oils met the Indonesian National Standard requirements except for the acid number of leatherlime essential oil and the optical rotation of rose essential oil. It is concluded that the combination of oils essential R. damacena and leather oil of C. amblycarpa  could reduce the total of microbes on air-conditioned rooms.

scholarly journals AKTIVITAS ANTIMIKROBA MINYAK ESENSIAL JAHE MERAH (Zingiber officinale var. Rubrum) DAN LENGKUAS MERAH (Alpinia purpurata K. Schum) TERHADAP BAKTERI PATOGEN DAN PERUSAK PANGAN

2015 ◽  
Vol 35 (01) ◽  
pp. 43 ◽  
Author(s):  
Tita Rialita ◽  
Winiati Pudji Rahayu ◽  
Lilis Nuraida ◽  
Budi Nurtama
Keyword(s):  
Antimicrobial Activity ◽  
Refractive Index ◽  
Essential Oil ◽  
Essential Oils ◽  
Optical Rotation ◽  
Acid Number ◽  
Food Spoilage ◽  
E Coli ◽  
Spoilage Bacteria ◽  
Red Ginger

The aims of this study was to determine the characteristics, composition and antimicrobial activity of essential oils of local Indonesian red ginger and red galangal against four pathogenic and food spoilage bacteria, which were B.cereus ATCC 10876, E. coli ATCC 25922, S. typhimuriumATCC 14028, and P. aeruginosa ATCC 27853. Analysis of physicochemical characteristics was carried outin accordance with ISO7355:1985. The chemical compositionwas analyzed using aGC-MS. The antimicrobial activity was determined by disc diffusion method and broth microdillution method was used for determine MIC and MBC values. Red ginger essential oil characteristic was brownish yellow, specific gravity 0.883, refractive index 1.480, optical rotation -8.45o, clear soluble (1:1) in 90 % alcohol, 2.06 acid number and 42.45 ester number. Redgalangal essential oil had a characteristic bright yellow color, specific gravity 0.895, refractive index 1.496, optical rotation -9.15o, clear soluble (1:1) in 90 % alcohol, 1.95 acid number and 140.15 ester number. The major component of red ginger essential oils were trimethyl-heptadien-ol, ar-curcumene, camphene, carbaldehyde, -sesquiphellandrene, and nerol; while the major component of red galangal essential oil were 1.8-cineole, chavicol, 9-desoxo-9-xi-hydroxy-3-pentaacetate-3,5,7,8,9,12-Ingol,- caryophyllene and -selinene. The essential oil of red ginger and red galangal hadmoderate antibacterial activity against pathogenic and food spoilage bacteria with the average inhibition zone 7.17-10.33 and 7.25-11.17mm.Red ginger essential oils could inhibit the growth of tested bacteria with MIC values of 2.65-3.97 mg/mL and MBC value of 3.10-5.29 mg/mL, while the red galangal essential oil could inhibit the growth of tested bacteria with MIC values of 1.79-4.03 mg mL and MBC values of 1.79-4.92 mg/mL. Based on the MIC and MBC values,all tested bacteriasensitivity to essential oils of red ginger and galangal red decline in a row B.cereus > E. coli > S. typhimurium> P. aeruginosa. Sensitivity of Gram positive and Gram negative bacteria to both essential oils demonstrate the potential of the oils to be used as a natural preservative in the food industry.Keywords: Antimicrobial, essential oil, red ginger, red galangal ABSTRAKPenelitian ini bertujuan untuk mengetahui karakteristik, komposisi dan aktivitas antimikroba minyak esensial jahe merah dan lengkuas merah lokal Indonesia terhadap empat spesies bakteri patogen dan perusak pangan, yaitu B.cereus ATCC 10876, E.coli ATCC 25922, S. typhimurium ATCC 14028, dan P. aeruginosa ATCC 27853. Analisis karakteristikfisika-kimia dilakukan sesuai standar ISO 7355:1985. Komposisi kimia dianalisis menggunakan alat GC-MS. Pengujian aktivitas antimikroba dilakukan dengan metode difusi cakram untuk menentukan zona hambat, sertabroth microdillution untuk menentukan nilai Minimum Inhibitory Concentration (MIC) dan Minimum Bactericidal Concentration (MBC).Karakteristik minyak esensial jahe merah yang dihasilkan yaitu kuning kecoklatan, berat jenis 0,883, indeks bias 1,480, putaran optik -8.45, larut jernih (1:1) dalam alkohol 90%, bilangan asam 2,06, dan bilangan ester 42,45. Minyak esensial lengkuas merah memiliki karakteristik warna kuning terang, berat jenis 0,895, indeks bias 1,496, putaran optik-9.15, larut jernih (1:1) dalam alkohol 90%, bilangan asam 1,95 dan bilangan ester 140,15. Komponen mayor minyak esensial jahe merah terdiri dari trimethyl-heptadien-ol, ar-curcumene, camphene, carbaldehyde, -sesquiphellandrene, dan nerol; sedangkan komponen mayor minyak esensial lengkuas merah terdiri dari 1.8-cineole, chavicol,9-desoxo9-xi-hydroxy-3,5,7,8,9,12-pentaacetat-ingol, -caryophyllenedan -selinene. Minyak esensial jahe merah dan lengkuas merah memiliki aktivitas antibakteri yang bersifat moderat terhadap bakteri patogen dan perusak pangan, dengan kisaran zona hambat rata-rata 7,17-10,33 mm dan 7,25-11,17 mm. Minyak esensial jahe merah dapat menghambatpertumbuhan bakteri uji pada nilai MIC 2,65-3,97 mg/mL dan nilai MBC 3,10-5,29 mg/mL, sedangkan minyak esensial lengkuas merah dapat menghambat bakteri uji dengan nilai MIC 1,79-4,03 mg/mL dan nilai MBC 1,79-4,92 mg/mL. Berdasarkan nilai MIC dan MBC, sensitivitas bakteri uji terhadap minyak esensial jahe merah dan lengkuas merah menurun berturut-turut dari B. cereus > E. coli > S. typhimurium > P. aeruginosa. Sensitivitas bakteri Gram positif dan Gram negatif terhadap kedua minyak esensial ini menunjukkan potensi minyak esensial jahe merah dan lengkuas merah untuk digunakan sebagai pengawet alami di industri pangan.Kata kunci: Antimikroba, jahe merah, lengkuas merah, minyak esensial

scholarly journals Quality Assessment of the Essential Oil from Eucalyptus globulus Labill of Blida (Algeria) Origin

2014 ◽  
Vol 36 ◽  
pp. 303-315 ◽  
Author(s):  
Mohamed Nadjib Boukhatem ◽  
Mohamed Amine Ferhat ◽  
Abdelkrim Kameli ◽  
Fairouz Saidi ◽  
Kerkadi Walid ◽  
...  
Keyword(s):  
Refractive Index ◽  
Essential Oil ◽  
Specific Gravity ◽  
Eucalyptus Globulus ◽  
Optical Rotation ◽  
Chemical Constituents ◽  
Chemical Properties ◽  
Acid Number ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components

Despite the reputation earned by aromatic and medicinal plants of Algeria, the chemical constituents of Eucalyptus globulus essential oil (EGEO) of Blida origin has not previously been investigated. Thus, the present study has been conducted for the determination of chemical constituents and different physico-chemical properties of the EGEO. Chemical composition of the EGEO, grown in Algeria, was analysed by Gas Chromatography-Mass Spectrometry. The chemical components were identified on the basis of Retention Time and comparing with mass spectral database of standard compounds. Relative amounts of detected compounds were calculated on the basis of GC peak areas. Fresh leaves of E. globulus on steam distillation yielded 0.96 % (v/w) of essential oil whereas the analysis resulted in the identification of a total of 11 constituents, 1.8 cineole (85.8 %), α-pinene (7.2 %), and β-myrcene (1.5 %) being the main components. Other notable compounds identified in the oil were β-pinene, limonene, α-phellandrene, γ-terpinene, linalool, pinocarveol, terpinen-4-ol, and α-terpineol. The physical properties such as specific gravity, refractive index and optical rotation and the chemical properties such as saponification value, acid number and iodine number of the EGEO were examined. The oil extracted has been analyzed to have 1.4602 - 1.4623 refractive index value, 0.918 - 0.919 specific gravity (sp.gr.), +9 - +10 optical rotation that satisfy the standards stipulated by European Pharmacopeia. All the physical and chemical parameters were in the range indicated by the ISO standards. Our findings will help to access the quality of the Eucalyptus oil which is important in the production of high value essential oils that will help to improve the economic condition of the community as well as the nation.

scholarly journals Characterization of patchouli oil (Pogostemon cablin Benth) production of Tinombala Village, Ongka Malino District, Parigi Moutong Regency

2021 ◽  
Vol 2126 (1) ◽  
pp. 012024
Author(s):  
P H Abram ◽  
W Putri ◽  
S Nuryanti ◽  
S M Sabang
Keyword(s):  
Refractive Index ◽  
Specific Gravity ◽  
Iron Content ◽  
Chemical Properties ◽  
Acid Number ◽  
National Standard ◽  
Patchouli Alcohol ◽  
Three Samples ◽  
Main Component

Abstract Patchouli oil comes from Tinombala village which has never been tested for the Indonesian National Standard (SNI). The purpose of this study was to determine the quality of patchouli oil by characterizing parameters of physical and chemical properties including color, refractive index, specific gravity, solubility in alcohol, acid number, ester number, iron content (Fe), and patchouli alcohol and alpha copaene content.. Sampling was done by random sampling. The results show a specific gravity of 25/25oC sample A = 0.9564, B = 0.9658, and C = 0.9590, refractive index (nD20) sample A = 1.5111, B = 1.5085 and C = 1.5086, the solubility in alcohol using ethanol of the three samples is >1:10 while using methanol 1:10, the acid number of the sample A=21.0375, B=9.1162 and C=19.6350, the ester number of the sample A=28,05, B=21.04 and C=21.04, iron (Fe) content of sample A=1.84210 mg/L, B=0.186842 mg/L and C=6.78950 mg/L, patchouli content alcohol sample A=,16,96%, B=15,52% and C=14,55% and for alpha copaene not detected. The results showed that the quality of patchouli oil in Tinombala village did not meet the Indonesian National Standard (SNI). Only the specific gravity, refractive index, and iron content are by the quality requirements, while the acid number and ester number and the main component determining the quality of patchouli oil, namely the patchouli alcohol content are still below the Indonesian National Standard, which is below 30%.

scholarly journals EKSTRAKSI MINYAK ATSIRI BUNGA MELATI DENGAN MENGGUNAKAN PELARUT ISOPROPIL ETER : PENGARUH WAKTU, TEMPERATUR, DAN RASIO MASSA BUNGA MELATI DENGAN VOLUME PELARUT

2019 ◽  
Vol 8 (1) ◽  
pp. 1-5
Author(s):  
Febrina Iskandar ◽  
Michael Dillo Rizki Ginting ◽  
Iriany ◽  
Okta Bani
Keyword(s):  
Refractive Index ◽  
Essential Oil ◽  
Oil Extraction ◽  
Acid Number ◽  
Extraction Time ◽  
Isopropyl Ether ◽  
Extraction Temperature ◽  
Index Analysis ◽  
Yellow Color ◽  
Solvent Lead

Jasmine flower is one of the high value commodities. One of the applications of  jasmine flower  is jasmine essential oil. Jasmine essential oil is often used to produce high quality parfume. Extraction is one of the methods for extracting jasmine essential oil, usually by the help of solvent. In this study, the effect of extraction time, extraction temperature, and solvent to jasmine flower ratio on jasmine oil extraction was studied. The extraction was carried out at 30oC, 35oC, and 40oC for 2 hours, 3 hours, 4 hours, and 5 hours by using isopropyl ether. The ratio of jasmine flower to isopropyl ether were 1:3; 1:4; and 1:5 (m/v). The resulting jasmine oil was analyzed by GC-MS and its quality was assessed according to SNI 06-2385-2006 which include colour test, refractive index analysis, acid number analysis, and ester number analysis. This experiment showed that increasing of extraction time, extraction temperature, and volume of solvent lead to increase of absolute yield of jasmine essential oil. The highest jasmine oil yield was obtained at 10.63 %. The jasmine oil contained 45.34% benzyl acetate and had yellow color. Its refractive index, acid number, and ester number were 1.485, 26.228 mg KOH/gram jasmine essential oil, and 159.885 mg KOH/gram jasmine essential oil.

scholarly journals Chemical profile and antioxidant, antibacterial, and cytotoxic activities on Artemia salina from the essential oil of leaves and xylopodium of Cochlospermum regium

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Roberta Heloísa de Paula Magalhães ◽  
Antonio Carlos Pereira de Menezes Filho ◽  
Matheus Vinicius Abadia Ventura ◽  
Hellen Regina Fernandes Batista-Ventura ◽  
Carlos Frederico de Souza Castro ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Refractive Index ◽  
Essential Oil ◽  
Relative Density ◽  
Optical Rotation ◽  
Artemia Salina ◽  
Cytotoxic Activities ◽  
Chemical Profile ◽  
Radical Reduction ◽  
Antibacterial Potential

Cochlospermum regium is a shrub plant species from the Cerrado domain used in traditional medicine. This study aimed to evaluate the chemical profile and antioxidant, antibacterial and cytotoxic activities on Artemia salina from the essential oil of fresh leaves and xylopodium. Fresch leaves and xylopodium of C. regium were collected in an area of ​​Cerrado domain in Goiás State, Brazil, 2021. The essential oil was obtained by hydrodistillation, the yield was quantified and the chemical profile determined by gas chromatography with mass spectrometry (GC-MS). Physicochemical analyzes were carried out for organoleptic analysis (color and appearance), solubility, relative density (g mL-1), refractive index, optical rotation (αD), antioxidant activity in DPPH radical reduction (IC50 µL mL-1), antibacterial activity on Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Salmonella serovar Enteritidis and Salmonella serovar Thyphymurium by the disc method (mm), and cytotoxicity bioassay on Artemia salina (LC50 µg mL-1). The major compounds for the essential oil of fresh leaves were viridiflorol 10.21%, Copaen-4-α-ol <β>, longiborneol 9.07 and β-bisabolene 11.48%, and for the essential oil of xylopodium β-selinene 26.17%, aromadendrene 8.66 % and thujopsene 8.09%. The yield was 0.58 and 0.33%, color slightly yellow and yellow for fresh leaves and xylopodium, respectively. Positive solubility, refractive index of 1.3468 and 1.3347, optical rotation +48.8 and +21.5, relative density 0.932 and 0.936 g mL-1, antioxidant activity IC50 = 47.65 and 111.16 µL mL-1 for fresh leaves essential oil and xylopodium, respectively. The essential oil from fresh leaves showed high antibacterial potential for all strains, as well as for cytotoxic activity on A. salina with LC50 = 90.17 and 625.08 µg mL-1, respectively.

scholarly journals Caracterização química e atividades antioxidante e antifúngica do óleo essencial das flores de [Cochlospermum regium (Mart. ex Schrank.) Pilger] (Bixaceae)

2020 ◽  
Vol 1 (52) ◽  
pp. 80
Author(s):  
Antonio Carlos Pereira de Menezes Filho ◽  
Wendel Cruvinel De Sousa ◽  
Carlos Frederico De Souza Castro
Keyword(s):  
Antioxidant Activity ◽  
Refractive Index ◽  
Antifungal Activity ◽  
Essential Oil ◽  
High Efficiency ◽  
Optical Rotation ◽  
Antioxidant Agent ◽  
Agricultural Use ◽  
Liquid Yield ◽  
Organoleptic Parameters

<p class="Normal1">The objective of this work was to characterize the chemical and physicochemical profile, and the antioxidant and antifungal activities of the essential oil from Cochlospermum regium flowers. The flowers were collected in a riparian Cerrado area. The essential oil was extracted by hidrodestilation in Clevenger. The organoleptic parameters were evalueted for (color, visual and aroma), for physicochemical, yield (%), solubility, optical rotation (αD ), refractive index (20 °C) and density (25 °C g mL-1), antioxidant activity were determined by the reduction of free radical DPPH, and the antifungal activity was determined through the percentage of growth inhibition on Sclerotinia sclerotiorum, Colletotrichum gloeosporioides and Aspergillus flavus. The essential oil from C. regium flowers had an oily, clear, crystalline and strongly aromatic liquid, yield of 0.099%, positive solubility, optical rotation +36.1 αD , refractive index 1.3297 20 °C, relative density of 0.933 g mL-1. The chemical profile showed the following major compounds ocimene&lt;(E)-β-&gt; with 15.87%, caryophyllene E 11.53%, γ-muurolene 20.07%, bicyclogermacrene 16.11% and, rosifoliol with 31.09%. The antioxidant activity showed high efficiency in reducing of the DPPH radical in all concentrations varied between 50 to 0.031 mg mL -1 between 95.25% to 40.09%. The antifungal activity showed 100% of mycelial inhibition in 100 and 50 µL mL-1 concentrations for S. sclerotinia, C. gloeosporioides and A. flavus. In the lowest concentration of 1.56 µL mL-1 inhibition was also observed for S. sclerotiorum and A. flavus and for 3.13 µL mL-1 for C. gloeosporioides with 26.12; 6.08 and 10.07%, respectively. The essential oil of the flowers from C. regium proved to be an efficient natural product for the development of medicines, as an antioxidant agent and, antifungal for agricultural use.</p>

scholarly journals Ekstraksi Minyak Atsiri Pandan Wangi (Pandanus amaryllifolius Roxb.) dengan Lama Waktu Penyulingan yang Berbeda

2020 ◽  
Vol 25 (4) ◽  
pp. 653-656
Author(s):  
Maya Damayanti ◽  
Sarifah Nurjanah ◽  
Anas Bunyamin ◽  
Totok Pujianto
Keyword(s):  
Refractive Index ◽  
Essential Oil ◽  
Specific Gravity ◽  
Steam Distillation ◽  
Descriptive Analysis ◽  
Quality Parameters ◽  
Acid Number ◽  
Added Value ◽  
Distillation Method ◽  
Pandanus Amaryllifolius

The leaves of pandan wangi (Pandanus amaryllifolius Roxb.) are known as food flavor and can be extracted to produce essential oil.  Extraction of its essential oil is usually conducted by distillation method that would give added value for this plant. This study aimed to study the effect of different durations of distillation process on the essential oils yield and quality of pandanus using water and steam distillation method. The method used in this research was experimental method with descriptive analysis. This research used five variants duration of distillation, which was repeated two times. The duration of distillation consisted of 2 hours, 4 hours, 6 hours, 8 hours, and 10 hours. The parameters observed to determine the best treatment consisted of the amount of yield produced and the quality parameters including specific gravity, refractive index, acid number, solubility in ethanol 90%, and ester number. Based on the results of the research conducted, distillation to produce the best yield is the duration of 8 hours with a yield value of 0.55%, while distillation to produce the best quality is the duration of 6 hours with a specific gravity of 1.0563 g/mL, refractive index 1.3473, the acid number was 3.274 mg KOH/g, solubility in ethanol 90% (1:1), and ester number of 96.06 mL HCl/g. Keywords: essential oil, pandan wangi, water and steam distillation

scholarly journals EKSTRAKSI MINYAK ATSIRI BUNGA MELATI: PENGARUH RASIO MASSA BUNGA MELATI DENGAN VOLUME PELARUT N-HEKSANA, WAKTU EKSTRAKSI, DAN TEMPERATUR EKSTRAKSI

2019 ◽  
Vol 8 (1) ◽  
pp. 42-47
Author(s):  
Michael Dillo Rizki Ginting ◽  
Febrina Iskandar ◽  
Iriany ◽  
Okta Bani
Keyword(s):  
Refractive Index ◽  
Essential Oil ◽  
Acid Number ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Index Analysis ◽  
Yellow Color ◽  
Original Plant ◽  
Solvent Lead ◽  
Jasminum Sambac

Jasmine flower (Jasminum sambac) contains essential oil compounds. Jasmine flower essential oil is a high-quality essential oil. Essential oils are volatile oils that have aroma similar to the original plant. Jasmine flower essential oil is sought due to its calming and anti-depression nature. In this study, the effect of extraction temperature, extraction time and solvent to jasmine flower ratio on jasmine oil was studied. The extraction was carried out at 30oC, 35oC, and 40oC for 2 hours, 3 hours, 4 hours, and 5 hours by using n-hexane. The ratio of jasmine flower to n-hexanes were 1:3; 1:4; and 1:5 (w/v). Resulting jasmine oil was analyzed by GC-MS, and its quality was assessed according to SNI 06-2385-2006 which include colour test, refractive index analysis, acid number analysis, and ester number analysis. This experiment showed that increasing of extraction time, extraction temperature, and volume of solvent lead to increase of absolute yield of jasmine essential oil. The highest jasmine oil yield obtained at 11.69 %. The jasmine oil contained was 47.14 % benzyl acetate and had yellow color. Its refractive index, acid number, and ester number were 1.478, 20.92 mg KOH/gram jasmine essential oil, and 150.27 mg KOH/gram jasmine essential oil respectively.

COUNTING TOTAL BACTERIA IN LAND ORGANIC WASTE HOUSEHOLD AND LAND INORGANIC WITH TOTAL PLATE COUNT METHOD (TPC)

2017 ◽  
Vol 4 (2) ◽  
pp. 87-91
Author(s):  
Ekamaida Ekamaida
Keyword(s):  
Soil Fertility ◽  
Bacterial Population ◽  
Organic Soil ◽  
Biological Properties ◽  
Plate Count ◽  
Bacterial Populations ◽  
Fertility Data ◽  
Plate Count Method ◽  
The Mean ◽  
Total Bacteria

The soil fertility aspect is characterized by the good biological properties of the soil. One important element of the soil biological properties is the bacterial population present in it. This research was conducted in the laboratory of Microbiology University of Malikussaleh in the May until June 2016. This study aims to determine the number of bacterial populations in soil organic and inorganic so that can be used as an indicator to know the level of soil fertility. Data analysis was done by T-Test that is by comparing the mean of observation parameter to each soil sample. The sampling method used is a composite method, which combines 9 of soil samples taken from 9 sample points on the same plot diagonally both on organic soil and inorganic soil. The results showed the highest bacterial population was found in total organic soil cfu 180500000 and total inorganic soil cfu 62.500.000

Pengaruh Suhu Penyimpanan terhadap Organoleptik, Derajat Keasaman dan Pertumbuhan Bakteri Coliform pada Susu Pasteurisasi

2016 ◽  
Vol 10 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Agustina Arianita Cahyaningtyas ◽  
Wiwik Pudjiastuti ◽  
Ilham Ramdhan
Keyword(s):  
Storage Temperature ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Pasteurized Milk ◽  
Initial Analysis ◽  
Total Plate Count ◽  
Plate Count Method ◽  
Pathogenic Microbes ◽  
Total Coliform Bacteria

One attempt to reduce the number of pathogenic microbes in milk is through the pasteurization process. This research aims to determine the effect of storage temperature on the organoleptic, acidity (pH) and growth of coliform bacteria in pasteurized milk. Pasteurized milk is stored at the varies of temperature  4°C (observed for 14 days), 10°C-15°C (observed for 14 days) and 25°C-27°C (observed for 22 hours), as well as also conducted an initial analysis pasteurized milk. The parameters were observed among other organoleptic (smell, taste, color, texture), pH and total coliform bacteria. Testing acidity using pH paper, while the growth of coliform bacteria testing done using Total Plate Count method based on ISO 2897 in 2008. The results of this study indicate that storage at 4°C for 14 days, organoleptic pasteurized milk is still good until the day ke- 8, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 3100x101 CFU / ml. Storage at 10°C-15°C for 14 days, organoleptic pasteurized milk is still good until the 6th day, the pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 5729x101 CFU / ml. Storage at 25°C-27°C for 22 days, organoleptic pasteurized milk is still good until the 9th, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 4.3 x106 CFU / ml.ABSTRAKSalah satu usaha untuk mengurangi jumlah mikroba patogen pada susu adalah melalui proses pasteurisasi. Penelitian ini bertujuan untuk mengetahui pengaruh suhu penyimpanan terhadap organoleptik, derajat keasaman (pH) dan pertumbuhan bakteri Coliform pada susu pasteurisasi. Susu pasteurisasi disimpan pada suhu yang bervariasi yaitu suhu 4°C (diamati selama 14 hari), suhu 10°C-15°C (diamati selama 14 hari) dan suhu 25°C-27°C (diamati selama 22 jam), serta dilakukan pula analisa awal susu pasteurisasi. Parameter yang diamati antara lain organoleptik (bau, rasa, warna, tekstur), pH dan jumlah bakteri Coliform. Pengujian derajat keasaman menggunakan kertas pH, sedangkan pengujian pertumbuhan bakteri Coliform dilakukan dengan menggunakan metode Total Plate Count berdasarkan SNI 2897 Tahun 2008. Hasil penelitian ini menunjukkan bahwa penyimpanan pada suhu 4°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai dengan hari ke-8, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 3100x101 Cfu/ml. Penyimpanan pada suhu 10°C-15°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai hari ke-6, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 5729x101 Cfu/ml. Penyimpanan pada suhu 25°C-27°C selama 22 hari, organoleptik susu pasteurisasi masih baik sampai jam ke-9, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 4,3 x106 Cfu/ml.Kata kunci : bakteri coliform, derajat keasaman, suhu penyimpanan, organoleptik, susu pasteurisasi

Sign in / Sign up

Export Citation Format

Share Document