ПОКАЗАТЕЛИ АКТИВИНА А У ПАЦИЕНТОК С НАРУЖНЫМ ГЕНИТАЛЬНЫМ ЭНДОМЕТРИОЗОМ. МАТЕРИАЛЫ 15-ГО МЕЖДУНАРОДНОГО МЕЖДИСЦИПЛИНАРНОГО КОНГРЕССА ПО АЛЛЕРГОЛОГИИ И ИММУНОЛОГИИ (22-24 МАЯ, 2019, МОСКВА)

2019 ◽  
Author(s):  
M.A. Levkovich ◽  
N.V. Ermolova ◽  
I.I. Krukier ◽  
T.G. Avanesova ◽  
A.A. Nikashina
Keyword(s):  
Blood Serum ◽  
Enzyme Immunoassay ◽  
Reproductive System ◽  
Peritoneal Fluid ◽  
Structural Changes ◽  
Activin A ◽  
Reproductive Age ◽  
Control Group ◽  
Systemic Level

Обоснование. В структуре гинекологической патологии наружный генитальный эндометриоз (НГЭ) занимает 3-е место, приводя к функциональным и структурным изменениям в репродуктивной системе и бесплодию. Цель. Определение содержания активина А на локальном и системном уровне у пациенток с наружным генитальным эндометриозом. Материалы и методы. Под наблюдением находилась 71 пациентка с НГЭ, которые были распределены на две группы: 1-я группа - пациентки с I-II стадиями (n31) 2-я - пациентки с III-IV стадиями (n40). В контрольную группу вошли 24 пациентки без НГЭ. Определение содержания активина А в сыворотке крови и перитонеальной жидкости проводили методом иммуноферментного анализа с использованием тест-систем DSL (США). Результаты. У пациенток в обеих исследуемых группах содержание активина А в сыворотке крови превысило показатели контрольной группы. Наиболее выраженные изменения отмечены у пациенток с III-IV стадией НГЭ. При исследовании перитонеальной жидкости обнаружено повышение активина А только у пациенток во 2-й группе. Заключение. Повышение содержания активина А в сыворотке крови и перитонеальной жидкости ассоциировано с риском развития НГЭ у пациенток репродуктивного возрастаActuality. External genital endometriosis (EGE), leading to functional and structural changes in the reproductive system and infertility, takes the 3rdplace in the structure of gynecological pathology. Objective. To determine the local and systemic level of activin A in patients with external genital endometriosis. Materials and methods. The study included 71 patients with EGE, who were divided into two groups: 1 group - patients with I-II stages (n31) 2 - patients with III-IV stages (n40). The control group included 24 patients without EGE. The determination of the level of activin A in the serum and peritoneal fluid was performed by enzyme immunoassay using DSL test systems (USA). Results. In the blood serum the level of activin A exceeded in patients of both studied groups compared the control group, but the most evident changes were observed in patients with stage III-IV of EGE. In the study of peritoneal fluid an increase in activin A was found only in patients in the 2ndgroup. Conclusion. An increase of serum and peritoneal fluid activin A level was associated with the risk of developing of EGE in patients of reproductive age.

scholarly journals Indicators of activin A in patients with external genital endometriosis. Materials of 15th International Interdisciplinary Congress on Allergology and Immunology (May 22-24, 2019, Moscow)

2019 ◽  
Vol 16 (2) ◽  
pp. 93-95
Author(s):  
M A Levkovich ◽  
N V Ermolova ◽  
I I Krukier ◽  
T G Avanesova ◽  
A A Nikashina
Keyword(s):  
Blood Serum ◽  
Enzyme Immunoassay ◽  
Reproductive System ◽  
Peritoneal Fluid ◽  
Structural Changes ◽  
Activin A ◽  
Reproductive Age ◽  
Control Group ◽  
Systemic Level

Actuality. External genital endometriosis (EGE), leading to functional and structural changes in the reproductive system and infertility, takes the 3rd place in the structure of gynecological pathology. Objective. To determine the local and systemic level of activin A in patients with external genital endometriosis. Materials and methods. The study included 71 patients with EGE, who were divided into two groups: 1 group - patients with I-II stages (n=31); 2 - patients with III-IV stages (n=40). The control group included 24 patients without EGE. The determination of the level of activin A in the serum and peritoneal fluid was performed by enzyme immunoassay using DSL test systems (USA). Results. In the blood serum the level of activin A exceeded in patients of both studied groups compared the control group, but the most evident changes were observed in patients with stage III-IV of EGE. In the study of peritoneal fluid an increase in activin A was found only in patients in the 2nd group. Conclusion. An increase of serum and peritoneal fluid activin A level was associated with the risk of developing of EGE in patients of reproductive age.

ПОКАЗАТЕЛИ АКТИВИНА А У ПАЦИЕНТОК С НАРУЖНЫМ ГЕНИТАЛЬНЫМ ЭНДОМЕТРИОЗОМ. МАТЕРИАЛЫ 15-ГО МЕЖДУНАРОДНОГО МЕЖДИСЦИПЛИНАРНОГО КОНГРЕССА ПО АЛЛЕРГОЛОГИИ И ИММУНОЛОГИИ (22-24 МАЯ, 2019, МОСКВА)

2019 ◽  
pp. 93-95
Author(s):  
M.A. Levkovich ◽  
N.V. Ermolova ◽  
I.I. Krukier ◽  
T.G. Avanesova ◽  
A.A. Nikashina
Keyword(s):  
Blood Serum ◽  
Enzyme Immunoassay ◽  
Reproductive System ◽  
Peritoneal Fluid ◽  
Structural Changes ◽  
Activin A ◽  
Reproductive Age ◽  
Control Group ◽  
Systemic Level

Обоснование. В структуре гинекологической патологии наружный генитальный эндометриоз (НГЭ) занимает 3е место, приводя к функциональным и структурным изменениям в репродуктивной системе и бесплодию. Цель. Определение содержания активина А на локальном и системном уровне у пациенток с наружным генитальным эндометриозом. Материалы и методы. Под наблюдением находилась 71 пациентка с НГЭ, которые были распределены на две группы: 1я группа пациентки с III стадиями (n31) 2я пациентки с IIIIV стадиями (n40). В контрольную группу вошли 24 пациентки без НГЭ. Определение содержания активина А в сыворотке крови и перитонеальной жидкости проводили методом иммуноферментного анализа с использованием тестсистем DSL (США). Результаты. У пациенток в обеих исследуемых группах содержание активина А в сыворотке крови превысило показатели контрольной группы. Наиболее выраженные изменения отмечены у пациенток с IIIIV стадией НГЭ. При исследовании перитонеальной жидкости обнаружено повышение активина А только у пациенток во 2й группе. Заключение. Повышение содержания активина А в сыворотке крови и перитонеальной жидкости ассоциировано с риском развития НГЭ у пациенток репродуктивного возрастаActuality. External genital endometriosis (EGE), leading to functional and structural changes in the reproductive system and infertility, takes the 3rdplace in the structure of gynecological pathology. Objective. To determine the local and systemic level of activin A in patients with external genital endometriosis. Materials and methods. The study included 71 patients with EGE, who were divided into two groups: 1 group patients with III stages (n31) 2 patients with IIIIV stages (n40). The control group included 24 patients without EGE. The determination of the level of activin A in the serum and peritoneal fluid was performed by enzyme immunoassay using DSL test systems (USA). Results. In the blood serum the level of activin A exceeded in patients of both studied groups compared the control group, but the most evident changes were observed in patients with stage IIIIV of EGE. In the study of peritoneal fluid an increase in activin A was found only in patients in the 2ndgroup. Conclusion. An increase of serum and peritoneal fluid activin A level was associated with the risk of developing of EGE in patients of reproductive age.

Prognostic value of levels of specific autoantibodies in patients of reproductive age with endometrial polyps

2021 ◽  
pp. 33-36
Author(s):  
L. V. Tkachenko ◽  
N. I. Sviridova ◽  
I. A. Gritsenko ◽  
S. N. Maksimov
Keyword(s):  
Blood Serum ◽  
Prognostic Value ◽  
Reference Range ◽  
Relative Content ◽  
Reproductive Age ◽  
Enzyme Linked Immunosorbent Assay ◽  
Women Of Reproductive Age ◽  
Endometrial Polyps ◽  
Double Stranded Dna

The problem of endometrial polyps in women of reproductive age is one of the urgent problems of modern gynecology. The detection rate of PE according to the data of domestic and foreign scientists reaches 25–35%, and therefore they occupy a leading place in the structure of intrauterine pathology.The aim. To study the levels of specific autoantibodies (to double-stranded DNA; to TrM‑03 antigens and collagen) and to assess their prognostic value for the risk of PE formation and recurrence in patients of reproductive age.Materials and methods. Examination of 86 patients aged 18 to 45 years (average age was 34.1 ± 6.3 years), admitted for hysteroscopy, hysteroresection of PE. Study of the relative content of specific autoantibodies to double-stranded DNA; to TrM‑03 antigens and collagen in blood serum was carried out by the method of enzyme-linked immunosorbent assay using specialized reagent kits (ELI-P-Complex).Results. In the course of this study, it was found that in the overwhelming majority of cases (65.1%) PEs were manifested by various types of AMC. All patients with PE were diagnosed with a significant decrease in the level of autoantibodies to double-stranded DNA, which is a marker of apoptosis processes, as well as a statistically significant decrease in the levels of autoantibodies to platelet antigens TrM‑03. The profile of deviations in the level of autoantibodies to TrM‑03 from the reference range in the area of negative values correlated with an increase in the average level of autoantibodies to collagen.Conclusions. Determination of the levels of auto-ATs to double-stranded DNA in serum can be used as a marker for predicting the recurrent course of PE. Determination of the levels of auto-ATs to platelet antigens TrM‑03 and to collagen in blood serum can be used as markers for the development of AMC by the type of BMC or a combination of BMC and BMC in patients with endometrial polyps.

scholarly journals FEATURES OF ENDOMETRIUM STRUCTURE IN ALCOHOL-ABUSING HIV-INFECTED INDIVIDUALS

2021 ◽  
Vol 8 (1) ◽  
pp. 52-58
Author(s):  
M. Lytvynenko
Keyword(s):  
Wall Thickness ◽  
Reproductive System ◽  
Morphological Changes ◽  
Average Diameter ◽  
Relative Volume ◽  
Reproductive Age ◽  
Maximum Diameter ◽  
Control Group ◽  
Minimum Diameter ◽  
Secretory Type

Background. Patients with comorbid pathology occupy leading positions in the practice of a doctor of any specialty especially in patients with HIV. Reproductive system is known to be the gateway for viruses. This fact could explain the severity of changes developing in the female reproductive system infected with HIV, in particular in the endometrium. The purpose of this study was to assess morphological changes in the endometrium caused by the combined effects of HIV infection and chronic alcoholism. Materials and methods: The study included sectional material taken from 60 women of reproductive age (20-40 years). They were all divided into two groups. The first group (30 people) consisted of HIV-positive individuals who, according to a survey of relatives and according to an autopsy (the main symptom is the presence of alcoholic cirrhosis of the liver), alcohol abuse was confirmed. The following parameters were determined: the average diameter of the endometrial glands (proliferative type), the minimum diameter of the endometrial glands (proliferative type), the maximum diameter of the endometrial glands (proliferative type), wall thickness (proliferative type), the relative volume of the epithelium (proliferative type), the average diameter of the glands (secretory type), the minimum diameter of the glands (secretory type), the maximum diameter of the glands (secretory type), the relative volume of the epithelium (secretory type), the thickness of the epithelium. Results. the average diameter of the endometrial glands (proliferative type) decreased from 51.71 ± 2.90 x 10-6 m in the comparison group to 39.42 ± 2.35 x 10-6 m in the HIV-infected group, which was 23.77%. The minimum diameter of the endometrial glands (proliferative type) reduced from 32.47 ± 1.83 x10-6 m to 27.13 ± 1.73x10-6 m (16.45%), the maximum diameter from 72.14 ± 2.21 x10-6 m to 63.84 ± 3.29 x10-6 m (11.5%). the relative volume of the epithelium (proliferative type) decreased by 5.41% (from 54.43 ± 1.79% in the study group to 49.02 ± 2.65% in the control group). The thickness of the uterine wall was also significantly reduced from 15.18 ± 1.60 x10-6 m to 14.52 ± 1.19 x10-6 m, which was 4.35%. The maximum volume of glands (secretory type) changed from 127.98 ± 2.10 x10-6 m to 97.18 ± 3.12 x10-6 m (24%). Changes by 3.6% were also observed when examining the wall thickness (from 13.02 ± 1.36 x10-6 m to 12.55 ± 1.68 x10-6 m). Conclusion. The study evaluated features of endometrial restructuring in alcohol-abusing HIV-infected women.

scholarly journals Influence of active and passive smoking at pregnancy on expression of an oxidizing stress

2019 ◽  
pp. 236-239
Author(s):  
O. A. Chursina ◽  
O. D. Konstantinova ◽  
S. I. Krasikov ◽  
A. A. Petrova ◽  
N. I. Kolosova
Keyword(s):  
Blood Serum ◽  
Pregnant Women ◽  
Passive Smoking ◽  
Rapid Test ◽  
Superoxide Dismutases ◽  
Control Group ◽  
Active Smoking ◽  
Smoking During Pregnancy ◽  
Definition Of

Objective: definition of indicators of system prooksidanty-antioxidants at active and passive smoking during pregnancy. Material and methods. On the basis of city clinic for women 39 pregnant women on the term of a gestation of 37 weeks are examined. Questioning, rapid test for identification of a kotinin in urine, determination of level of a malon dialdehyde (MDA) and also activities superoxide dismutases (SOD) and catalases is carried out to bloods of surveyed. Patients are divided into 3 groups: I-of 11 smoking pregnant women subject to II-13 to passive smoking at pregnancy, III-control group of 15 women. Results. At patients of I and II groups substantial increase of level MDA in blood serum is noted. Reliable decrease of the activity of SOD of erythrocytes in the I group and insignificant decrease of the activity in II is taped. The indicator of catalase/SOD was statistically higher at active smoking. Conclusion. Active and passive smoking at pregnancy leads to change in prooxidatic and antioxidatic systems.

scholarly journals The importance of serum immunoglobulin free light chain assessment for predicting outcome in patients with newly diagnosed multiple myeloma in real clinical practice

2020 ◽  
Vol 15 (3) ◽  
pp. 38-50
Author(s):  
N. V. Skvortsova ◽  
I. B. Kovynev ◽  
K. V. Halzov ◽  
T. I. Pospelova
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Clinical Practice ◽  
Blood Serum ◽  
Control Group ◽  
Newly Diagnosed ◽  
Lactate Dehydrogenase Activity ◽  
Immunochemical Study ◽  
Real Clinical Practice

Background. The prognosis of patients with multiple myeloma (MM) is significantly different depending on the biological characteristics of the tumor substrate, the microenvironment of the bone marrow, as well as factors associated with the patient’s body. Therefore, the search for new reliable and easily identifiable prognostic markers is relevant for the effective management of patients with this disease.The objective of the study was to assess the prognostic value of the study of serum free light chains (FLC) of immunoglobulins κ and λ and their ratio κ / λ FLC in the blood serum of patients with newly diagnosed MM in real clinical practice.Materials and methods. 369 patients with first diagnosed MM (134 men and 235 women) were examined who were hospitalized in the hematology department of the City Clinical Hospital No. 2 Novosibirsk in the period since January 2012 to December 2017. The median age of the patients was 67 (32–82) years. All patients received induction courses of chemotherapy based on bortezomib. The control group consisted of 56 conditionally healthy individuals: 34 women (60.7 %) and 22 (39.3 %) men with a median age of 62 (40–68) years. The concentration of FLC-κ and FLC-λ (mg / L) in blood serum was determined by immunoturbidimetric method on a Hitachi 911 automated biochemical analyzer using the Freelite Human Lambda and Freelite Human Kappa reagent kits (Binding Site, Great Britain).Results. It was found that in patients with MM, the concentration of serum FLC-κ or FLC-λ was statistically significantly higher compared to the control group and varied depending on the type of MM (p <0.001). The diagnostic sensitivity of the quantitative determination of FLC and their ratio for MM was 98.64 %, compared with 94.04 % in a standard immunochemical study. The values of the ratio κ / λ FLC <0.04 or> 65, as well as the concentration of FLC-κ and FLC-λ are higher than the median obtained in the whole group (FLC-κ ≥702 mg / L and FLC-λ ≥493.2 mg / L), correlate with known factors of poor prognosis for MM (with a high concentration of β2‑microglobulin (>3.5 mg / L) (r = 0.461; p <0.001), plasma cell bone marrow infiltration >60 % (r = 0.420; p <0.001), renal failure (creatinine >177 μmol / L) (r = 0.380; p = 0.002), and also with high lactate dehydrogenase activity (>450 U / L) (r = 0.520; p <0.001) and is associated with poor outcomes. The median overall survival in the group of patients with κ / λ FLC <0.04 or >65 was 49 months compared to 76 months in the group with κ / λ FLC 0.04–65 (log-rank p = 0.012).Conclusion. The determination of free FLC in the blood serum of patients with MM can be used to assess the prognosis of their survival. The value of the κ / λ FLC ratio <0.04 or >65 allows us to divide patients with MM into risk groups with significantly different outcomes and can be used to identify patients at high risk who need more aggressive therapy and more detailed monitoring of the response.

scholarly journals Chromogranin A and chromogranin B in pancreatic neuroendocrine tumors

2020 ◽  
pp. 26-28
Author(s):  
N. V. Lyubimova ◽  
Yu. S. Timofeev ◽  
A. V. Lebedeva ◽  
N. E. Kushlinsky
Keyword(s):  
Blood Serum ◽  
Neuroendocrine Tumors ◽  
Chromogranin A ◽  
Diagnostic Sensitivity ◽  
Control Group ◽  
Pancreatic Neuroendocrine Tumors ◽  
Chromogranin B ◽  
Tumor Dissemination ◽  
First Time

For the first time in Russia a comparative study of chromogranin A (CgA) and chromogranin B (CgB) in neuroendocrine tumors (NETs) of the pancreas was performed. We examined 50 primary patients with pancreatic NETs and 42 healthy people. The determination of CgA and CgB was performed in blood serum using standard enzyme-linked immunoassay test-systems (Chromogranin A NEOLISA, Eurodiagnostica; Human Chromogranin B, USCN). The levels of CgA and CgB in pancreatic NETs significantly differed from the control group. There was found the association between CgA levels and the dissemination of the process, while CgB demonstrated the properties of a marker independent from the tumor dissemination. The diagnostic sensitivity of CgA was 76 %, CgB – 68 %. Complex determination of CgA and CgB enhanced the diagnostic sensitivity to 84 %. Our data indicate the potential usefulness of complex CgA and CgB in the diagnosis of pancreatic NETs.

Medical aspects of the pregnant women with cervical insufficiency and infertility in anamnesis

2020 ◽  
pp. 48-50
Author(s):  
O.M. Perkhulyn ◽  
Keyword(s):  
Pregnant Women ◽  
Reproductive System ◽  
Reproductive Technologies ◽  
Overweight And Obesity ◽  
Reproductive Age ◽  
High Rate ◽  
Control Group ◽  
Cervical Insufficiency ◽  
Basic Group ◽  
Anovulatory Infertility

The formation of the puberty period is an important background for the female reproductive system in the future and the realization of the childbirth. The objective: to assess medical aspects in women with cervical insufficiency (CI) and infertility associated with anovulation in history. Materials and methods. 60 pregnant women with CI and anovulatory infertility in anamnesis formed the basic group. In these persons the pregnancy occurred after the use of additional reproductive technologies. The control group formed 30 pregnant women without CI and infertility and with physiological pregnancy. Results. In 33.33% individuals in the basic group menarche started in 16 years old and more. While in the control group in all women menarche started in 11–15 years old (χ2=11.00; p<0.001). In the reproductive age all controls (100.00%) had regular menstrual cycle that was in 3.33 times more than the women in the basic group (30.00%; χ2=36.61; p<0.001), 70.00% persons in the basic group had irregular menstruations. The menstruations were always irregular from menarche in 16.64% persons with CI vs none cases among healthy women (χ2=4.06; p=0.04). Endometriosis was diagnosed in 48.33% individuals with CI and infertility, polycystic ovary syndrome – 38.33%, diminished ovarian reserve – 26.67%, hyperandrogenism – 41.67%, ovary cyst – 20.00%. The rate of the infections of the low genital tract before pregnancy was higher in women in the basic group, also 45.00% of these women had gynecological operations (χ2=19.43; p<0.001); thyroid diseases – 25.00% (χ2=5.03; p=0.02), overweight and obesity – 26.67%. Conclusion. Thus, the results of our study indicate that in the persons with cervical infertility and anovulatory infertility in anamnesis disorders in the reproductive system mostly start from the puberty period; the high rate of gynecological diseases, operations on the pelvic organs, pregnancy loss are typical for them. Key words: infertility, cervical insufficiency, factors.

Activity of eosinophils and immunoglobulin E concentration in the peritoneal fluid of women with endometriosis

2004 ◽  
Vol 42 (6) ◽  
Author(s):  
Androne Eidukaite ◽  
Vytas Tamosiunas
Keyword(s):  
Flow Cytometry ◽  
Enzyme Immunoassay ◽  
Peripheral Blood ◽  
Peritoneal Fluid ◽  
Immunoglobulin E ◽  
Abdominal Cavity ◽  
Control Group ◽  
Study Group ◽  
Autoantibody Production ◽  
Healthy Control

AbstractAutoinflammatory phenomena, including autoantibody production and atopy, have been regarded as associated with endometriosis. The present study investigates the activity of eosinophils and the distribution of immunoglobulin E concentrations in the peritoneal fluid of women with early endometriosis. The study group consisted of 30 patients with laparoscopically diagnosed early endometriosis. The healthy control group consisted of 18 females with no evident changes in the abdominal cavity and no endometrial foci. Concentrations of immunoglobulin E in serum and peritoneal blood were determined by enzyme immunoassay. The activity of eosinophils was estimated according to the expression of the early activation molecule CD69 by the flow cytometry method. The concentrations of immunoglobulin E in the peripheral blood and peritoneal fluid were similar in both groups. However, the count of CD69+ eosinophils was higher in the peritoneal fluid of women with endometriosis. The results indicate that activated eosinophils accumulate in the peritoneal fluid in early endometriosis and can play a significant role in the pathogenesis of the disease.

scholarly journals Determination of Cholesterol and Triglyceride Concentrations in Serum Extracellular Vesicles Using Commercial Kits

2021 ◽  
Vol 4 (2) ◽  
pp. e00148
Author(s):  
A.A. Yakovlev ◽  
V.D. Antonov ◽  
T.A. Druzhkova ◽  
A.B. Guekht ◽  
N.V. Gulyaeva
Keyword(s):  
Blood Serum ◽  
Healthy Volunteers ◽  
Extracellular Vesicles ◽  
The Body ◽  
Control Group ◽  
Sensitivity Threshold ◽  
Commercial Kits ◽  
Average Size

Exosomes and microvesicles, collectively referred to as small extracellular vesicles (sEV) are vesicles with an average size of about 100-150 nm. Currently, the role of sEV in various aspects of signaling in the body is being actively investigated; in addition, sEV can often serve as markers of various pathologies. The active study of the sEV composition is continuing. In this study we have demonstrated that in sEV it is possible to determine cholesterol and triglycerides concentration by using commercial kits designed for serum. The technique was tested on sEV from the blood of patients diagnosed with depression and on healthy volunteers. No differences were found in the concentration of cholesterol and triglycerides in mEV from the blood serum of depressed patients and the control group. The concentration of cholesterol and triglycerides in the samples is several times higher than the sensitivity threshold of the methods set by the manufacturer of the kits.

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