THE PATOMORFOLOGICAL STUDIES OF RATS POIISONED WITH CYANOPYRETHROID AND USAGE OF THERAPEUTIC DRUGS

2021 ◽  
Vol 1 (3) ◽  
pp. 343-349
Author(s):  
G.R. Yamalova ◽  
◽  
K.F. Khalikova ◽  
A.V. Malanev ◽  
D.V. Aleуev ◽  
...  
Keyword(s):  
Therapeutic Agent ◽  
Lethal Dose ◽  
Sedative Effect ◽  
Sodium Bromide ◽  
Medicinal Products ◽  
Internal Organs ◽  
Animal Body ◽  
Therapeutic Drugs ◽  
White Rats ◽  
Negative Effect

Experiments were conducted to study the effect of medicinal products consisting of dipyroxime, sodium bromide, pyridoxine and phenazepam with diosmectite on pathomorphological changes in the internal organs of white rats with β-cypermethrin poisoning in an average lethal dose. The results of the study showed that the most pronounced effect of the pesticide has on the liver, kidneys, and brain. Necrobiological and dystrophic changes were noted. The use of a therapeutic agent consisting of dipyroxime, sodium bromide and pyridoxine leads to a decrease in the negative effect of the pesticide on the liver, brain and kidneys. The use of phenazepam and diosmectite leads to a sedative effect on animals, and promotes the adsorption and excretion of β-cypermethrin from the animal body.

scholarly journals STUDY OF ACUTE TOXICITY OF A NEW VETERINARY DRUG FOR INTTRAMMARY INTRODUCTION

2018 ◽  
Vol 2 ◽  
pp. 51-60
Author(s):  
Zhanna Polova
Keyword(s):  
Acute Toxicity ◽  
Lethal Dose ◽  
Dosage Forms ◽  
Veterinary Drug ◽  
Test Substance ◽  
Medicinal Products ◽  
Preclinical Research ◽  
Toxic Compounds ◽  
White Rats ◽  
Veterinary Medicinal Products

Preclinical studies of veterinary medicinal products are important and compulsory studies in the development of new dosage forms. The aim of preclinical research is to determine the toxic effect and therapeutic efficacy of the test substance-the future dosage form, its effect on the body's basic systems, as well as the identification of possible side effects. This work is part of the research on the development of the composition and technology of the veterinary drug - a solution for intramammary application, conventionally called "Argocide", intended for the treatment of mastitis in cattle. A study of the acute toxicity of the intramammary veterinary drug was carried out in experiments on white rats of both sexes, according to the requirements for potential medicines. The establishment of the value of the average lethal dose (LD50) of the veterinary drug "Argocide" with intramuscular single administration to white mature rats is impossible due to the absence of animal death even when the drug is administered at doses exceeding 5.0 ml/kg. This experiment allows the veterinary preparation "Argocide" to be classified as practically non-toxic compounds (V class). The analysis of the results of the conducted studies indicates the relative harmlessness of the potential drug for veterinary medicine and allows us to foresee that the "Argocide" preparation can be classified as low-risk substances, which justifies the expediency of its further study and introduction into practice.

STUDY OF DRUGS EFFICIENCY FOR TREATMENT AT THE POISONING OF WHITE RATS BY NEONICOTHYNOID PESTICIDES

2018 ◽  
Vol 1 (1) ◽  
pp. 90-94
Author(s):  
V . I. Egorov ◽  
◽  
K. F. Khalikovа ◽  
G. R. Yamalova ◽  
D. V. Aleev ◽  
...  
Keyword(s):  
Survival Rate ◽  
Magnesium Sulfate ◽  
Water Intake ◽  
Therapeutic Agent ◽  
Therapeutic Agents ◽  
Sodium Bromide ◽  
Clinical State ◽  
Control Group ◽  
White Rats ◽  
Biochemical Studies

The article presents the results of clinical-hematological, biochemical and pathomorphological studies of white rats with thiaclopridic poisoning when using drugs. The animals of the control group received tiacloprid intragastrically, the rats of the experimental groups – thiacloprid and therapeutic agents (magnesium sulfate, novocaine, xymedon, diproxime, sodium bromide, glucose). In the course of the experiments, the clinical state of animals, feed and water intake, survival and pathological anatomical picture were studied. To determine hematological and biochemical studies, blood of the rats was taken by decapitation. Based on the results of the studies, it has been established that the combination of magnesium sulfate and novocaine is most effective as a therapeutic agent for poisoning animals with thiacloprid. As a result, the survival rate of animals was 83,3%. The effectiveness of treatment is confirmed by hematological, biochemical and pathomorphological studies.

The Effect of Sodium Nitrite on Experimental Animals

1942 ◽  
Vol 6a (1) ◽  
pp. 63-73 ◽  
Author(s):  
H. L. A. Tarr ◽  
N. M. Carter
Keyword(s):  
Sodium Nitrite ◽  
Lethal Dose ◽  
Oral Route ◽  
Female Rats ◽  
Male Rats ◽  
Subcutaneous Route ◽  
Healthy Male ◽  
Experimental Animals ◽  
White Rats ◽  
Healthy Female

Incorporation of sodium nitrite in the diet of cats and white rats on the basis of an average sized man consuming 1 lb. (454 g.) of fish containing 0.2 per cent (908 mg.) of this salt daily for six days each week does not appear to affect their growth rate nor the development (weight) of their thyroid, heart, lungs, spleen, liver, kidneys or adrenals. The fecundity of white rats as judged by their ability to breed and raise normal litters is apparently not affected thereby. The lethal dose of sodium nitrite by oral route is about 1.1 to 2.0 g./kg. for healthy male rats, 0.46 to 1.2 g./kg. for healthy female rats and 0.073 g./kg. for cats (one animal). The lethal dose by subcutaneous route is about 0.19 to 0.20 g./kg. for healthy male rats and 0.057 to 0.13 g./kg. for healthy female rats.

THE INFLUENCE OF SORPTION-DETOXIFYING MEANS ON CUMULATION OF 90SR IN BONE TISSUE OF WHITE RATES WITH COMBINED INTAKE OF 137CS, CD AND PB

2018 ◽  
Vol 1 (3) ◽  
pp. 129-133
Author(s):  
L. L. Zakharova ◽  
◽  
G. A. Zhorov ◽  
V. N. Obryvin ◽  
◽  
...  
Keyword(s):  
Bone Tissue ◽  
Chemical Nature ◽  
Mechanisms Of Action ◽  
The Body ◽  
Biologically Active ◽  
Laboratory Studies ◽  
Animal Body ◽  
White Rats ◽  
Combined Use ◽  
Active Substances

The article presents the results of laboratory studies of the effect of sorption-detoxifying means on the accumulation of 90Sr in the body of white rats. The efficiency of a number of selective and polyfunctional sorbents, detoxicants and other biologically active substances and the developed on their basis sorption-detoxifying complexes as means of efferent therapy and detoxification of the animal body at the combined intake of xenobiotics of radiation and chemical nature was evaluated. It was found that 90Sr cumulation in bones (at the level of 53,3–60,8%) was reduced to the greatest extent with the combined use of substances of different mechanisms of action and origin. The effectiveness of separate use of detoxifying drugs did not exceed 29,1%.

Mitochondria as a potential target for the development of prophylactic and therapeutic drugs against Schistosoma mansoni infection

2021 ◽  
Author(s):  
Keith Kiplangat Talaam ◽  
Daniel Ken Inaoka ◽  
Takeshi Hatta ◽  
Daigo Tsubokawa ◽  
Naotoshi Tsuji ◽  
...  
Keyword(s):  
Drug Development ◽  
Schistosoma Mansoni ◽  
Worm Burden ◽  
Consumption Rate ◽  
Therapeutic Agent ◽  
Ex Vivo ◽  
Prophylactic Agent ◽  
Therapeutic Drugs ◽  
Pyrvinium Pamoate

Emergence of parasites resistant to praziquantel, the only therapeutic agent, and its ineffectiveness as a prophylactic agent (inactive against the migratory/juvenile Schistosoma mansoni ), makes the development of new antischistosomal drugs urgent. The parasite’s mitochondrion is an attractive target for drug development because this organelle is essential for survival throughout the parasite’s life cycle. We investigated the effects of 116 compounds against Schistosoma mansoni cercariae motility that have been reported to affect mitochondria-related processes in other organisms. Next, eight compounds plus two controls (mefloquine and praziquantel) were selected and assayed against motility of schistosomula ( in vitro ) and adults ( ex vivo ). Prophylactic and therapeutic assays were performed using infected mouse models. Inhibition of oxygen consumption rate (OCR) was assayed using Seahorse XFe24 Analyzer. All selected compounds showed excellent prophylactic activity, reducing the worm burden in the lungs to less than 15% that obtained in the vehicle control. Notably, ascofuranone showed the highest activity with a 98% reduction of the worm burden, suggesting the potential for development of ascofuranone as a prophylactic agent. The worm burden of infected mice with S. mansoni at the adult stage was reduced by more than 50% in mice treated with mefloquine, nitazoxanide, amiodarone, ascofuranone, pyrvinium pamoate, or plumbagin. Moreover, adult mitochondrial OCR was severely inhibited by ascofuranone, atovaquone, and nitazoxanide, while pyrvinium pamoate inhibited both mitochondrial and non-mitochondrial OCRs. These results demonstrate that the mitochondria of S. mansoni are feasible target for drug development.

EVALUATION OF TOXIOLOGICAL INDICATORS OF MONTHMORRILLONITE CLAY FOR THE PURPOSE OF USING THEM AS ALUMOSILICATE SOBENT AGAINST THE NEGATIVE EFFECT OF MYCOTOXINS ON THE BODY OF ANIMALS AND BIRDS

2021 ◽  
Vol 1 (1) ◽  
pp. 72-78
Keyword(s):  
Experimental Studies ◽  
The Body ◽  
Montmorillonite Clay ◽  
Test Substance ◽  
Negative Effects ◽  
Experimental Animals ◽  
Eyelid Edema ◽  
White Rats ◽  
Mucous Membranes ◽  
Negative Effect

The article presents the results of laboratory studies to assess the toxicological parameters of montmorillonite clay from the Pogadaevskoye deposit in the West Kazakhstan region in order to use them as an aluminosilicate sorbent in the composition of feeds that reduce the negative effects of mycotoxins on the body of animals and birds. The relevance of research is associated with the cultivation of healthy and highly productive animals and poultry in order to ensure the food security of the Republic of Kazakhstan.The studies carried out to assess the toxicological parameters of montmorillonite clay in order to use them as an aluminosilicate sorbent in experimental animals (rabbits and white rats) allowed the following results to be obtained: Visual study of the intensity of erythema when exposed to the test substance on the skin of rabbits showed their absence (0 points). The study of the intensity of edema (an increase in the thickness of the skin clutch of rabbits) when exposed to the test substance on the skin of rabbits showed no reaction (0 points). Studies evaluating the irritating effect of the test substances on the mucous membranes of the eyes of rabbits by symptoms of damage showed the absence of hyperemia (0 points). Weak eyelid edema (1 point), the minimum amount of discharge in the corner of the eye (1 point). The results of studies on the classification assessment of the test substance for the severity of the irritant effect on the eyes of rabbits showed that the average total score of the severity of the irritative effect corresponds to 1 point. A comprehensive analysis of the results obtained on the basis of scientific and experimental studies to assess the toxicological indicators of montmorillonite clay from the Pogadaevskoye deposit in relation to irritating effects on the skin and mucous membranes of experimental animals (rabbits) showed their harmlessness.

INFLUENCE OF FODDER ADDITIVE ABIOTONIC ON VETERINARY AND SANITARY INDICATORS OF RABBIT MEAT

2019 ◽  
Vol 1 (4) ◽  
pp. 375-381
Author(s):  
F.I. Vasilevich ◽  
◽  
V.N. Shevkoplyas ◽  
V.M. Bachinskaya ◽  
◽  
...  
Keyword(s):  
Amino Acids ◽  
Physiological State ◽  
Essential Amino Acids ◽  
Feed Additive ◽  
Feed Supplement ◽  
Internal Organs ◽  
White Breed ◽  
Negative Effect ◽  
Rabbit Meat ◽  
White Fat

The materials on the study of the feed additive Abiotonic in rabbit breeding, as well as the results of organoleptic, microbiological, histological studies and the amino acid composition of rabbit meat of the New Zealand white breed are presented. According to the results of the studies, there was no negative effect of the feed additive on the veterinary and sanitary indicators of rabbit meat, the carcasses were well bled, drying crusts were formed in a timely manner, the muscles were elastic, light pink, white fat, the broth was clear and aromatic. No pathogenic and conditionally pathogenic microflora were found. At the same time, it was noted that the feed additive contributed to the increase in meat of non-essential amino acids by 6,01%, essential amino acids by 9,74%, which increases the biological value of the product. According to histomorphological studies, the Abiotonic feed supplement did not adversely affect the physiological state of animals, muscle tissue and internal organs developed within the physiological norm of rabbits.

PECULIARITIES OF THE RADIATION PATTERN OF THE BRAIN AND INTERNAL ORGANS OF NEWBORNS WHO UNDERWENT SEVERE PERINATAL ASPHYXIA AND UNDERWENT GENERAL THERAPEUTIC HYPOTHERMIA, ACCORDING TO THE PILOT STUDY RESULTS

2021 ◽  
Vol 100 (1) ◽  
pp. 30-35
Author(s):  
N.S. Vorotyntseva ◽  
◽  
V.V. Orlova ◽  
A.N. Motina ◽  
A.D. Novikova ◽  
...  
Keyword(s):  
Therapeutic Hypothermia ◽  
Perinatal Asphyxia ◽  
Control Group ◽  
Internal Organs ◽  
X Ray ◽  
Abdominal Organs ◽  
Study Results ◽  
Negative Effect ◽  
Chest X Ray ◽  
The Brain

Objective of the research: to study the features of the radiological picture of the brain and internal organs of newborns with severe perinatal asphyxia, who underwent general therapeutic hypothermia (GTH). Materials and methods: the study included 116 newborns with severe perinatal asphyxia. GTH was performed in 72 patients (group 1), 44 children did not receive hypothermia in the 2 (control) group. In the first 6 hours of life, children of groups 1 and 2 underwent ultrasound of the brain and abdominal organs and chest x-ray. The complex ultrasound scan was repeated at 3–5, 7–10, 14–16 and 21–28 days. Repeated chest x-ray was carried out strictly according to the indications. Results: GTH reduced the incidence of organic brain lesions by 18% in children with severe perinatal asphyxia (p0,05). The study revealed a number of undesirable consequences associated with GTH. Transient effusion into the abdominal and thoracic cavities was diagnosed by ultrasound in 8 (11%) patients after hypothermia, while there were no such changes in children in the control group (p<0,05). X-ray of the chest in newborns who received GTH during the first 14 days revealed edematous-hemorrhagic changes in the lungs more often than in patients of the control group – 55 (76%) and 24 (55%), respectively (p<0,05). Conclusions: GTH effectively prevented the development of severe post-hypoxic changes in the brain. The negative effect of hypothermia on microcirculation was manifested by the development of effusion into the serous cavities and edematous-hemorrhagic syndrome.

Herbal medicinal products as an alternative for the treatment of minor sleep disorders and mild anxiety symptoms? Proof of concept in animal models

2014 ◽  
Vol 29 (S3) ◽  
pp. 657-657
Author(s):  
I. Heulard ◽  
M. Verleye
Keyword(s):  
Animal Models ◽  
Sleep Disorders ◽  
Sedative Effect ◽  
Anxiety Symptoms ◽  
Medicinal Products ◽  
Melissa Officinalis ◽  
General Activity ◽  
Herbal Medicinal Products ◽  
Herbal Medicinal ◽  
Dose Dependent

Herbal medicinal products have been traditionally used for the treatment of minor sleep disorders and mild anxiety symptoms. However, prospective clinical studies are scarce, due to a number of variability factors such as population heterogeneity and a strong placebo effect. To overcome these limitations, animal models are available that provide unbiased scientific results.In this study, we examined the effect of an association of four compounds [namely Melissa officinalis, Crataegus oxyancantha, calcium lactate and magnesium thiosulfate (32/30/32/6; w/w)], on several behavioral tests in rodents. Animals were tested for general activity on an automated actimetry system, and for anxiety on the so-called “staircase test”.In the actimetry test, adult mice were placed in an empty cage and their spontaneous level of activity (distance travelled and the number of rearings) was measured after an intraperitoneal administration of 25, 50 or 75 mg/kg of the association. A dose-dependent decrease of the distance travelled and number of rearings was observed after administration (statistically significant at the dose of 75 mg/kg). In the staircase test, the number of rearings (a measure of anxiety) and the number of steps climbed (general activity) were measured. A statistically significant decrease in the number of rearings was observed at the dose of 25 mg/kg (compared to vehicle-treated animals), whereas no effect in the number of steps climbed was observed at the same dose. These results suggest that the association could induce an anxiolytic effect. At higher doses, a sedative effect was observed.In conclusion, the administration of an association of Melissa officinalis, Crataegus oxyacantha, calcium and magnesium induced a clear dose-dependent sedative effect in mice. One test suggests that this association could also have anxiolytic properties. This study confirms that herbal medicinal products could be a beneficial treatment for minor sleep disorders or mild anxiety symptoms.

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