scholarly journals FRAKSI ETANOL EKSTRAK KULIT PISANG KEPOK KUNING ( Musa balbisiana) TERHADAP BAKTERI Staphylococcus epidermidis, Staphylococcus aureus, dan Propionibacterium acnes

2021 ◽  
Vol 10 (1) ◽  
pp. 10-15
Author(s):  
Yuli Wahyu Tri Mulyani ◽  
Akhmad Rokiban ◽  
Galih Cipto Mahendra
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Extraction Process ◽  
Negative Control ◽  
Banana Peel ◽  
Musa Balbisiana ◽  
Peel Extract ◽  
Ethanol Fraction

Yellow kepok banana peel (Musa balbisiana) contains antibacterial compounds against S. epidermidis, S. aureus and P.acne. This study aims to proved the antibacterial activity of the yellow kepok banana peel extract fractionated using ethanol as a solvent against S.epidermidis, S.aureus and P.acne. The yellow kepok banana peel extraction process was carried out by maceration method with 70% ethanol followed by fractionation to obtain ethanol fraction. Antibacterial activity testing using nutrient agar (NA) media with wells method with a concentration of 25,000 ppm, 50,000 ppm, 100,000 ppm, aquadest as a negative control and clindamycin as a positive control. The phytochemical test of the ethanol fraction showed the content of alkaloids, flavonoids, saponins, tannins and quinones. The results of the inhibition zone diameter of the ethanol fraction at a concentration of 100,000 ppm against S. epidermidis (11.87 mm), S. aureus (12.04 mm) and P. acne (11.35 mm). The test is to determined the minimum inhibitory concentration (MIC) the minimum inhibitory concentration value of the ethanol fraction of S. epidermidis, S. aureus is 20,000 ppm and for P. acne is 17,500 ppm. The conclusion of this studied was that the ethanol fraction of kepok yellow banana peel extract has inhibitory and bacteriostatic properties against S. epidermidis, S. aureus and P.acne bacteria.Keywords: alkaloids,Ant ibacter ial , flavonoids , Fractionated, Musa balbisiana

scholarly journals AKTIVITAS SEDIAAN GEL KUERSETIN TERHADAP Staphylococcus Epidermidis

2015 ◽  
Vol 1 (1) ◽  
pp. 59-64
Author(s):  
Rina Apriana Herslambang ◽  
Dina Rahmawanty ◽  
Mia Fitriana
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Phenolic Compound ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Sebaceous Glands ◽  
Agar Diffusion ◽  
Agar Diffusion Method

Acne is the abnormality production of sebum in the sebaceous glands which are usually caused by Staphylococcus epidermidis. Staphylococcus epidermidis one of bacterial that caused acne. Quercetin have a phenolic compound that can inhibit the growth of Staphylococcus epidermidis at 0.05% w/w. The purpose of this study was to determine the minimum inhibitory concentration of quercetin gel. HPMC was used as gel base. The concentrations of quercetin that was added to gel were 0.05% w/w, 0.15% w/w, and 0.25% w/w. The antibacterial activity of the gels was determined using agar diffusion method. The results showed that the inhibition zone on the F1, F2, and F3 were 7.83 mm , 6.53 mm , 4.56 mm and the result showed that quercetin gels significantly effected the growth of Staphylococcus epidermidis (p<0,05). The minimum inhibitory concentration of quercetin gel was 0,05% w/w with inhibition zone of 7.83 mm.

scholarly journals Antibacterial Activity Of Tangerine Powder (Citrus reticulata Blanco.) Against Caries-Causing Bacteria (Streptococcus mutans)

2020 ◽  
Vol 10 (2) ◽  
pp. 148-157
Author(s):  
Framesti Frisma Sriarumtias ◽  
Aji Najihudin ◽  
Nopi Rantika ◽  
Rita Nengsih
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Pharmaceutical Ingredients ◽  
Caries Treatment ◽  
Inhibitory Power

Dental caries is caused by Streptococcus mutans, which lives in the oral cavity and causes plaque on the teeth. Dental caries treatment involves restoration or filling of tooth tissue that has been perforated due to dental caries. The purpose of this study was analyze the effectivity of  tangerine juice powder in preventing the emergence of dental caries caused by Streptococcus mutans. The experimental method was used in this study started by making a powder from tangerine jiuce using freeze drying method, followed by testing the antibacterial activity using the disc diffusion method. In addition, the minimum inhibitory concentration (MIC) was also sought by measuring the diameter of the clear zone. The results showed that tangerine powder has antibacterial activity, with a minimum inhibitory concentration of 10%. The highest activity of tangerine powder to inhibit the growth of Streptococcus mutans bacteria was observed at   concentration of 25% with an inhibition zone diameter of 15.8 mm, and is classified as having moderate inhibitory power. According to the result of this study is that tangerine juice powder has a good antibacterial activity against Streptococcus mutans and can be developed into a pharmaceutical ingredients to prevent dental caries.

scholarly journals IN VITRO AND IN SILICO ANTIBACTERIAL ACTIVITY OF 1.5-BIS (3’-ETHOXY-4’-HYDROXYPHENYL)-1-4-PENTADIENE-3-ONE

2018 ◽  
Vol 10 (5) ◽  
pp. 70
Author(s):  
Agus Purwanggana ◽  
Esti Mumpuni ◽  
Esti Mulatsari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Staphylococcus Epidermidis ◽  
In Silico ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Antibacterial Study

Objective: The main objective of this research were screened in vitro and in silico of 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one as potential antibacterial agents.Methods: The in vitro antibacterial study was carried against Staphylococcus aureus, Staphylococcus epidermidis (gram positive) and Escherichia coli, Salmonella thypi (gram negative) using broth dilution method to determine Minimum Inhibitory Concentration (MIC), disc diffusion method to determine the diameter of inhibition zone. In silico antibacterial study was carried using computational software Protein-Ligand ANT System (PLANTS), computational docking was carried using receptor with Protein Data Bank (PDB) file 3MZD. The structures were optimized prior docking using YASARA, and MarvinSketch. The results of antibacterial testing were compared to two positive control drugs i. e amoxicillin and cefadroxil.Results: In vitro evaluation showed that 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has a better antibacterial activity than amoxicillin and cefadroxil with a Minimum Inhibitory Concentration (MIC) of 0.15 ppm and diameter of inhibition zone of 11.27±0.31, 11.35±0.39, 11.25±0.33, and 11.05±0.45 mm in Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Salmonella thypi, respectively. These results in line with in silico evaluation that showed 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has more negative docking score than amoxicillin, cefadroxil, and cloxacillin acyl as a native ligand on the 3MZD receptor.Conclusion: This results obtained in this research work were 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one compound potential as an antibacterial agent. 

scholarly journals Isolation and Characterization of Antibacterial Actinomycetes from Soil Samples of Kalapatthar, Mount Everest Region

1970 ◽  
Vol 10 ◽  
pp. 173-182 ◽  
Author(s):  
Tara Devi Gurung ◽  
Chringma Sherpa ◽  
Vishwanath Prasad Agrawal ◽  
Binod Lekhak
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Thin Layer ◽  
Broad Spectrum ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Group Method ◽  
Mount Everest ◽  
Primary Screening ◽  
Isolation And Characterization

Seventy-nine Actinomycetes were isolated from soils of Kalapatthar (5545m), Mount Everest region. Twenty seven (34.18%) of the isolates showed an antibacterial activity against at least one test-bacteria among two Gram positive and nine Gram negative bacteria in primary screening by perpendicular streak method. Thirteen (48.15%) showed antibacterial activity in secondary screening. The result showed that three of the isolates, K.6.3, K.14.2, and K.58.5 were highly active with an inhibition zone e"20mm and broad spectrum antibacterial activity including two methicillin resistant Staphylococcus aureus (MRSA) strains. Minimum inhibitory concentration (MIC) of antibacterial metabolites of the isolate K.6.3 was 1mg/ml, and that of isolates K.14.2 and K.58.5 was 2mg/ml. Two spots were detected on thin layer chromatography plate from each of the metabolites which was completely different from the spot produced by vancomycin. The active isolates from primary screening were heterogeneous in their overall macroscopic, biochemical, and physiological characteristics through unweighted pair group method using average (UPGMA) cluster analysis. Delineation of the three active isolates showing potent broad spectrum antibacterial activity revealed that they belonged to distinct taxonomic groups.Key words: Antibacterial activity; Minimum inhibitory concentration; Thin layer chromatographyDOI: 10.3126/njst.v10i0.2957Nepal Journal of Science and Technology Vol. 10, 2009 Page: 173-182 

scholarly journals Antibacterial Activity Test of Ethanol Extract Pineapple (Ananas comosus (L.) Merr.) Peel against Growth of Propionibacterium acnes

2019 ◽  
Vol 2 (2) ◽  
pp. 108-113
Author(s):  
Fitriyanti Fitriyanti ◽  
Muhammad Nur Rahman Hendrawan ◽  
Karunita Ika Astuti
Keyword(s):  
Antibacterial Activity ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Ananas Comosus ◽  
Treatment Groups ◽  
Pineapple Peel ◽  
Pineapple Fruit ◽  
Peel Extract

Pineapple peel (Ananas comosus (L.) Merr.) is waste from the pineapple fruit. The purpose of this study was to determine the content of pineapple peel extract and to antibacterial activity in various concentrations effective in inhibiting the growth of Propionibacterium acnes. The pineapple peel extract is made by using the extraction method in the form of maceration. The method used in the inhibitory test using the three replication samples in each treatment group. The sample consisted of 10 treatment groups ie pineapple peel extract concentration 12.5%, 25%, 37.5%, 50%, 62.5%, 75%, 87.5%, and 100%, as well as positive and negative control. The results obtained from pineapple peel screening contain flavonoid and saponin compounds. The data analysis shows that pineapple peel extract concentrations of 50%, 62.5%, 75%, 87.5%, and 100% had inhibitory zones with the medium-strong category while clindamycin as the control has a strong inhibition zone. The conclusion of this research proves that pineapple peel extract has antibacterial power to P. acnes with effective concentration is 100% with the strong category.

scholarly journals PENGARUH EKSTRAK BIJI Ganitri (Elaeocarpus sphaericus Schum.) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli

2020 ◽  
Vol 2 (01) ◽  
pp. 28-37
Author(s):  
Ega Bramaseta ◽  
Tristi Indah Dwi Kurnia ◽  
Hasyim As'ari
Keyword(s):  
Escherichia Coli ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Seed Extract ◽  
Growth Of Bacteria

This research was conducted in the laboratory of Biology University PGRI Banyuwangi to determine the effect of ganitri seeds extract (Elaeocarpus sphaericus Schum.) in inhibiting the growth of bacteria Escherichia coli and to determine the Minimum Inhibitory Concentration (MIC) of ganitri seeds extract (Elaeocarpus sphaericus Schum.). The method is using wells with 1% tetracycline positive control and negative control of sterile distilled water. Serial concentrations ganitri seed extract (Elaeocarpus sphaericus Schum.) is used by 10%, 20%, 30%, 40%, and 50%, and in the MIC test using serial concentrations of 1%, 2.5%, 5%, 7.5% and 10%. Based on the test results, the largest inhibition zone is shown by a concentration of 50% with an average diameter of 0,954 cm zone of inhibition and inhibition zone indicated smallest concentration of 10% with an average diameter of 0,215 cm inhibition zone. ANOVA test result value (F.Hit> F.Table) with F.Hit amounted to 33,512 and F.Tabel significance value of 2.85 and 0.000 (P <0.05), as the value of P <0.05 then it can be concluded that there is a concentration of ganitri seeds extract (Elaeocarpus sphaericus Schum.) on the growth of test bacteria. The results of Duncan test showed the ganitri seeds extract (Elaeocarpus sphaericus Schum.) at a concentration of 50% have significantly different inhibatory zone by treatment with 10%, 20%, 30%, 40%, positive control, and negative control. While MIC ganitri seed ethanol extract (Elaeocarpus sphaericus Schum.) Were still able to inhibit the growth of test bacteria at a concentration of 1% with an average diameter of 0.037 cm inhibition zone. The Minimum Inhibitory concentration (MIC) ganitri seed extract (Elaeocarpus sphaericus Schum.) shown by a concentration of 1%, where this concentration is still able to inhibit the growth of bacteria Escherichia coli.  Keyword: Ganitri seed extract, Bacteria Escherichia coli, Serial concentration, Minimum Inhibitory concentration (MIC), Inhibition zone.

scholarly journals Antibacterial Activity of Fractions from Extract Ethanolic of Hylocereus Polyrhizus Peel Against E. Coli and S. aureus

2021 ◽  
Vol 1 (2) ◽  
Author(s):  
Tsania Khusnul Khotimah ◽  
Annisa Krisridwany ◽  
Salmah Orbayinah ◽  
Sabtanti Harimurti
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Ethyl Acetate Fraction ◽  
Negative Control ◽  
Hexane Fraction ◽  
E Coli ◽  
Ethanol Fraction ◽  
Hylocereus Polyrhizus

Peel of red dragon fruit (Hylocereus polyrhizus) is one of the plants used as an antibacterial agent as it contains saponin triterpenoid compounds, flavonoid compounds, and alkaloid compounds which can have antibacterial activity. This research aims to determine the antibacterial effect of n-hexane, ethyl acetate, and ethanol fraction of red dragon fruit’s peel against Escherichia coli and Staphylococcus aureus by the concentration of 10mg/ml, 20mg/ml, 40mg/ml, 80mg/ml dan 160mg/ml. This research was conducted by using laboratory experiments. The simplicia was macerated with 96% ethanol and fractionated by n-hexane and ethyl acetate. The phytochemical screening of the fraction was n-hexane fraction containing saponin and alkaloid, while the ethyl acetate fraction contained saponin and flavonoid. Kanamycin was used as a positive control, while DMSO was used as a negative control. According to this research, the MIC value of ethanol fraction, n-hexane fraction, and ethyl acetate fraction were 80mg/ml, 20mg/ml, and 80mg/ml, respectively, for E. coli and all fractions were 10mg/ml for S. aureus. Based on the average diameter of the inhibition zone, the largest diameter zone in E. coli was ethyl acetate fraction with 160mg/ml concentration  that was  10,33mm. Meanwhile, in S. aureus n-hexane fraction, it was 160mg/ml, which was 11,20mm. This result showed that the n-hexane fraction has good gram-positive activity while the ethyl acetate fraction has good activity on gram-negative.

In Vitro Antibacterial activity of ethanolic extract of Myrsine africana against laboratory Strains of Pathogenic Organisms

2016 ◽  
Vol 5 (04) ◽  
pp. 4512
Author(s):  
Jackie K. Obey ◽  
Anthoney Swamy T* ◽  
Lasiti Timothy ◽  
Makani Rachel
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
E Coli ◽  
Zones Of Inhibition ◽  
In Vitro Antibacterial Activity ◽  
Myrsine Africana

The determination of the antibacterial activity (zone of inhibition) and minimum inhibitory concentration of medicinal plants a crucial step in drug development. In this study, the antibacterial activity and minimum inhibitory concentration of the ethanol extract of Myrsine africana were determined for Escherichia coli, Bacillus cereus, Staphylococcus epidermidis and Streptococcus pneumoniae. The zones of inhibition (mm±S.E) of 500mg/ml of M. africana ethanol extract were 22.00± 0.00 for E. coli,20.33 ±0.33 for B. cereus,25.00± 0.00 for S. epidermidis and 18. 17±0.17 for S. pneumoniae. The minimum inhibitory concentration(MIC) is the minimum dose required to inhibit growth a microorganism. Upon further double dilution of the 500mg/ml of M. africana extract, MIC was obtained for each organism. The MIC for E. coli, B. cereus, S. epidermidis and S. pneumoniae were 7.81mg/ml, 7.81mg/ml, 15.63mg/ml and 15.63mg/ml respectively. Crude extracts are considered active when they inhibit microorganisms with zones of inhibition of 8mm and above. Therefore, this study has shown that the ethanol extract of M. africana can control the growth of the four organisms tested.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN MEKAI (Pycnarrhena cauliflora DIELS.) TERHADAP Staphylococcus aureus

2020 ◽  
Vol 6 (2) ◽  
pp. 246
Author(s):  
Yayuk Bulam Sarifati ◽  
Sjarif Ismail ◽  
Khemasili Kosala
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Statistical Tests ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Control Measurement ◽  
Eye Pain

Mekai leaves (Pycnarrhena cauliflora Diels.) (P. cauliflora). Are known to contain flavonoid compounds, tannins and phenolics that act as antibacterial agents and are used in the treatment of eye pain. Staphylococcus aureus (S. aureus) is one of the examples of bacterial diseases of eye pain and also a major cause of many infections in communities and health facilities with cases of resistance to various antimicrobial agents. The purpose of this study was to prove the antibacterial activity of mekai leaves ethanol extract against S. aureus bacteria. This research is an experimental research. The stages of this research began by extracting mekai leaves using maceration method with 96% ethanol solvent. Antibacterial activity was tested by the disc method (Kirby-Bauer) using ethanol extract concentrations of mekai leaves (EPC) 20%, 30%, 40%, 50%, 60%, 70%, and 80%, positive control using 25 μg amoxicillin and negative control using DMSO 10%. The measurement results of inhibition zones of mekai leaf ethanol extract 20%, 30%, 40%, 50%, 60%, 70% and 80% respectively were 8.32 mm, 8.32 mm, 8.32 mm, 8.67 mm, 9.00 mm, 8.67 mm, and 8.33 mm. While the positive control measurement of 25 μg amoxicillin against S. aureus is 28.67 mm and the measurement of 10% negative DMSO control does not produce inhibitory zones, so it can be concluded that the ethanol extract of mekai leaves has antibacterial activity, but the area of ​​inhibition zone produced is smaller than amoxicillin 25 µg. The results of statistical tests using Mann Whitney between negative controls with all EPC concentrations obtained significant differences with p values ​​<0.05, it can be concluded that there is antibacterial activity produced at all EPC concentrations.

In-Vitro Studies on the Sensitivity of Staphylococcus aureus to Some Ethno-Medicinal Preparations Sold around Kano, Nigeria

2011 ◽  
Vol 4 (1) ◽  
pp. 22-25 ◽  
Author(s):  
M Bashir ◽  
I Yusuf ◽  
AS Kutama
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Test Organism ◽  
Ethanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Herbal Preparations

Five traditional herbal preparations were sampled between May-June, 2009 in Kano. The samples were investigated for invitro antibacterial activities against clinical isolates of Staphylococcus aureus. Likewise, phytochemical screening tests were conducted to determine some of the phytochemicals present in the ethanolic and water extracts of the samples. Various concentrations of the extracts were prepared using serial doubling dilutions (5000=l/ml, 2500=g/ml, 1250=g/ml, 625=g/ml and 312.5=g/ml). All the test extracts showed slight antibacterial activity against the test organism, with ethanolic extract of sample E having the highest zone diameter of inhibition, while sample H had the lowest diameter of inhibition. The standard antibiotic disc (Gentamicin) had demonstrated the highest activity on the test organisms. The results of the Phytochemical screening revealed the presence of steroid in all the samples, tannin in samples A, C, D and E, reducing sugars in sample A, D and E respectively. The result of the minimum inhibitory concentration (MIC) was found to be above 312.5=g/ml for samples C, D and E. Keywords: Staphylococcus aureus, Herbal preparations, antibacterial activity, Phytochemical screening and minimum inhibitory concentration.

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