scholarly journals Phytochemical, Antimicrobial and Ethnobotanical Study of Calotropis gigantea

2020 ◽  
Vol 10 (2) ◽  
pp. 23-27
Author(s):  
Hari Timilsina ◽  
Bindu Modi ◽  
Ramchandra Basnyat
Keyword(s):  
Antimicrobial Activity ◽  
Research Work ◽  
Herbal Medicines ◽  
Polar Compounds ◽  
Common Disease ◽  
Ethnobotanical Study ◽  
Phytochemical Screening ◽  
Ethnobotanical Survey ◽  
Calotropis Gigantea ◽  
Hexane Extracts

Introduction: According to the WHO, more than 80% of world’s population depends upon the traditional medicine for primary care of health. The increased interest in plant derived drugs is mainly because of ‘herbal medicines’ are safer than costly synthetic drug. In this study, Calotropis gigantea also known as 'Aank' in Nepali was selected as the plant for the research work. Plants were collected from different parts of the Chitwan district. The objective of the study was to prepare the methanol and hexane extracts of leaves and stem of C. gigantea and carry out phytochemical screening of those extracts. Similarly, antimicrobial activity of those extract were evaluated to find its potential as drug. The ethnobotanical survey was done to find out medicinal values of the plant. Methods: Methanol and hexane extracts of the leaves and stem of C. gigantea was prepared by Soxhlet extraction method. Methanol was used for the extraction of various polar compounds and hexane for non-polar compounds. Phytochemical screening results showed the presence of secondary metabolites such as alkaloids, glycosides, flavonoids and terpenoids. The phytochemicals present in different plant extracts were analyzed by following the protocol given by Ciulei I. Inhibition of bacterial growth was tested by using agar well diffusion plate method (As per DPR/BS/SOP/ Am/1) and measured in the form of zone of inhibition (ZOI). Results: The ZOI shown by methanol extracts of leaves of C. gigantea for Escherichia coli and Staphylococcus aureus were measured 8 mm and 13 mm respectively. Similarly, the zones of inhibition shown by hexane extract of leaves of C. gigantea for E.coli and S.aureus were measured as 11 mm and 9 mm respectively in antibacterial assay. Antimicrobial activity was not seen against Klebsiella pneumoniae by any of the extracts. The ethnobotanical study conducted showed that the plant is being used for different medicinal purposes. Conclusion : The findings of the study showed that the plant had high pharmaceutical importance. Traditionally, it is used alone or with other medicinal plants to treat common disease such as asthma, swelling rheumatism, diarrhoea, dysentery, syphilis, ulcer, leprosy etc. This study hopes to provide valuable information for different research.

scholarly journals Production of Antiseptic Tablet Aloe Vera Soap

2020 ◽  
Vol 5 (8) ◽  
pp. 612-615
Author(s):  
Muhammad Sulaiman Rahama ◽  
Abubakar Sani
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Research Work ◽  
Aloe Vera ◽  
Reducing Sugar ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Commercial Soap ◽  
Phytochemical Constituents

This research work aimed at investigating some phytochemical constituents present in aloe vera based antiseptic soap and its activity against some selected microorganisms. The soap was produced using hot process and the antimicrobial activity was performed against Staphylococcus aureus, Escherichia Coli and Candida albicans using the method of agar well diffusion. The produced soap exhibited highest zone of inhibition on Staphylococcus aureus with 8.6 mm, 10.1 mm, 13.8 mm and 15.1 mm at 25 mg/mL, 50 mg/mL, 100 mg/mL and 200 mg/mL respectively, no inhibition was observed on E. Coli while the C. albicans was slightly inhibited by the soap by 7.6 mm, 9.0 mm, 13.2 mm at 50 mg/mL, 100 mg/mL, 200mg/mL respectively. The phytochemical screening conducted confirmed the presence of tannins, saponins, reducing sugar and flavonoid in the plant extract. The results of the investigations clearly indicated that aloe vera based soap had possessed antimicrobial activity against the tested microorganisms due to the presence of the confirmned phytoconstituents. Likewise pH, formability, and antimicrobial activity of the produced soap were comparable with the commercial soap.

scholarly journals Echinacea purpurea (L.) Moench: Chemical Characterization and Bioactivity of Its Extracts and Fractions

2020 ◽  
Vol 13 (6) ◽  
pp. 125
Author(s):  
Joana Coelho ◽  
Lillian Barros ◽  
Maria Inês Dias ◽  
Tiane C. Finimundy ◽  
Joana S. Amaral ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Cytotoxic Activity ◽  
Chemical Characterization ◽  
Echinacea Purpurea ◽  
Polar Compounds ◽  
Aerial Parts ◽  
Organic Extracts ◽  
Hexane Extracts ◽  
Medicinal Properties ◽  
Acetone Extracts

Echinacea purpurea (L.) Moench is widely known for its medicinal properties, being one of the most used medicinal plants for its immunostimulant properties. Nevertheless, there is still scarce information on its cytotoxic activity. Thus, this study aims at evaluating the cytotoxicity and antimicrobial activity of several aqueous and organic extracts of the aerial parts of this plant and chemically characterizing the obtained extracts. The analysis was performed by HPLC–DAD–ESI/MS. Fifteen compounds were identified; of these, seven were phenolic acids and eight were flavonoids. Non-polar compounds were evaluated by GC/MS, with a total of sixty-four compounds identified, and the most abundant groups were the sterols, fatty acids and long-chain hydrocarbons. The highest antimicrobial activity was exhibited by the dichloromethane, ethyl acetate, and acetone extracts. Dichloromethane and n-hexane extracts showed the highest cytotoxic activity. Therefore, they were fractionated, and the obtained fractions were also assessed for their cytotoxicity. Notwithstanding, the cytotoxicity of the extracts was superior to that of the obtained fractions, evidencing a possible synergistic effect of different compounds in the whole extracts.

scholarly journals Ethnobotanical Study of Fig Tree (Ficus Carica L.) And Olive (Olea Europaea L.) From Tetouan Province In Morocco And Study Their Antimicrobial Activity

2021 ◽  
Vol 319 ◽  
pp. 01091
Author(s):  
Zakaria Mennane ◽  
Zahira Tabet ◽  
Btissam El kharraz ◽  
Touria Aabid ◽  
Touil Souhaila ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Viral Infections ◽  
Ethnobotanical Study ◽  
Inhibitory Effects ◽  
Ethnobotanical Survey ◽  
Black Olive ◽  
Fig Tree ◽  
Ethanol Extracts ◽  
Insect Bites

This study we have conducted an ethnobotanical survey and antimicrobial activity in Tetouan province to identify the use of the famous plant species in traditional medicine : fig tree and Olive and follow-up of antimicrobial activity during three stages for green, pink and black olives and mixing activity of olive and fig extracts. 80 questionnaire sheets were done during April-June 2019, and the method of diffusion was used for the antimicrobial activity.Bacteria and yeasts were used: Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae, E.coli and Candida albicans. The results revealed that 32% of farmers use olive oil for the initial treatment of respiratory diseases, and 22% use it for digestive system problems and 13% use it as a treatment of the nervous system.However, fig tree is used against insect bites, as an inhibitor of the growth of certain cancerous tumors, diabetes and certain viral infections. The ethanol extracts showed good inhibitory effects against most strains of bacteria and yeast. Also for the majority of strains tested, it was found that in December (black olive), activity is stronger, and that the mixture of ethanol extracts with ethanol extracts of fig tree it strengthened them.

Evaluation of antimicrobial potential of some spices from apiaceae sold in herbal shops

2019 ◽  
Author(s):  
Alev ONDER ◽  
Suna Sibel GURPINAR, Mujde ERYILMAZ ◽  
Bayram Kagan AKAY, Ahsen Sevde CINAR
Keyword(s):  
Antimicrobial Activity ◽  
Daucus Carota ◽  
Antimicrobial Effect ◽  
Coriandrum Sativum ◽  
Apium Graveolens ◽  
Dilution Method ◽  
Anethum Graveolens ◽  
Foeniculum Vulgare ◽  
Pimpinella Anisum ◽  
Hexane Extracts

Spices are a part of the plants used for many purposes as preservatives and as colorants in foods or as medicinal intention. Main aim of the present research was to estimate the potential antimicrobial activity of some spices from Apiaceae family such as Amni visnaga (Diş otu, Hıltan), Anethum graveolens (Dereotu), Apium graveolens (Kereviz), Coriandrum sativum (Kişniş), Cuminum cyminum (Kimyon), Daucus carota (Havuç), Foeniculum vulgare (Rezene), Petroselinum sativum (Maydanoz), Pimpinella anisum (Anason). Thus, the fruits of the plants are used in the experiments. The fruits have been extracted by n-hexane, and all extracts have been subjected to TLC (Thin Layer Chromatography). The n-hexane extracts were screened for their potential in vitro antibacterial activity against Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853 and antifungal activity against Candida albicans ATCC 10231 by microbroth dilution method. The hexane extracts of the fruits of Coriandrum sativum, Anethum graveolens, Daucus carota, and Pimpinella anisum did not show antimicrobial activity against tested microorganisms. Except these, the other extracts having MIC values of 2.5-5-10 mg/mL exhibited antimicrobial effect against some tested microorganisms. These results demonstrate that the extracts which have an antimicrobial effect can probably play a role as an antimicrobial agent owing to their nonpolar components which are accumulated to the n-hexane extracts.

Assessment ofOpuntia ficus-indicain vivo Following Ethnobotanical Survey: Confirmation of its Analgesic Activity

2018 ◽  
Vol 16 (S1) ◽  
pp. S191-S196
Author(s):  
A. Mouhaddach ◽  
A. El-hadi ◽  
K. Taghzouti ◽  
M. Bendaou ◽  
R. Hassikou
Keyword(s):  
Body Weight ◽  
Analgesic Activity ◽  
Prickly Pear ◽  
Tail Flick ◽  
Ethnobotanical Study ◽  
Ethnobotanical Survey ◽  
Hot Plate ◽  
Traditional Use ◽  
Opuntia Ficus Indica ◽  
Plant Parts

Opuntia ficus-indica(the cactus or prickly pear) is a cactus belonging to the Opuntiae family. Several Opuntiae plant parts have been used in traditional Moroccan medicine. In this study, we investigated its most common use as an analgesic. An ethnobotanical study ofOpuntia ficus-indicawas first conducted in 10 areas in Morocco. Extracts fromOpuntia ficus-indicacladodes were obtained using a decoction method and its analgesic activity in mice was investigated by the hot plate and tail flick methods. Cladode extracts had significant (p<0.05) analgesic activity at intraperitoneal doses of 300, 500, and 1000 mg/kg body weight. Both methods revealed significantly increased latency at all three doses (p<0.05) compared to controls. These data suggest that the traditional use of this plant as an analgesic is valid; in fact, perhaps it may be a centrally-acting analgesic.

Phytochemical screening and antimicrobial activity of Salvia sharifii Rech. & Esfand from Iran

2021 ◽  
pp. 1-6
Author(s):  
Zahra Aghaee ◽  
Ardalan Alizadeh ◽  
Mehrzad Honarvar ◽  
Ramin Babadaei Samani
Keyword(s):  
Antimicrobial Activity ◽  
Phytochemical Screening

scholarly journals Antioxidant, Antimicrobial Activities and Fatty Acid Components of Flower, Leaf, Stem and Seed of Hypericum scabrum

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Ali Shafaghat
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Hexane Extracts ◽  
Hypericum Scabrum ◽  
Different Parts

The hexane extracts of flower, leaf, stem, and seed of Hypericum scabrum, which were collected from northwestern Iran, were obtained by extraction in a Soxhlet apparatus. The fatty acids were converted to methyl esters and determined by gas chromatography/flame ionization detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS) systems. The hexane extract from the flower, leaf, stem, and seed contained 39.1%, 43.2%, 29.0%, and 37.6% of omega-3 fatty acids, respectively. The other main components of the flower extract were tetracosane (12.2%) and palmitic acid (9.3%), and that of the leaf extract was palmitic acid (7.4%). The stem and seed extracts contained bis(2-ethylhexyl)phthalate (18.7% and 35.7%), nonacosane (11.7% and 3.9%) and linoleic acid (6.5% and 6.9%) as major components. The hexane extracts of different parts from H. scabrum represent an important source of omega-3 fatty acids in several Hypericum species. The antioxidant activity of all hexane extracts was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. The results indicate that hexane extracts from different parts of H. scabrum possess considerable antioxidant activity. The highest radical scavenging activity was detected in seed, which had an IC50 = 165 μg/mL. The antimicrobial activity of the extracts of those samples were determined against seven Gram-positive and Gram-negative bacteria ( Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae), as well as three fungi ( Candida albicans, Saccharomyces cerevisiae, and Aspergillus niger). The bioassay showed that the oil exhibited moderate antimicrobial activity. This study reveals that the all parts of this plant are attractive sources of fatty acid components, especially the essential ones, as well as of effective natural antioxidants.

Determination of the minimum suppressive concentration of photosensitizers of the second generation in relation to sanitaryindicative microorganisms

2021 ◽  
Vol 1 (11) ◽  
pp. 42-46
Author(s):  
Vadim Yu. Shlenskiy ◽  
◽  
Tatiana I. Pashnik ◽  
Svetlana Yu. Pigina ◽  
◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Salmonella Enteritidis ◽  
Second Generation ◽  
Research Work ◽  
Protoporphyrin Ix ◽  
Bactericidal Effect ◽  
Disodium Salt ◽  
Bacterial Cultures

In the studies described in the article, the presence of antimicrobial activity in photosensitizers in experiments on bacterial cultures was confirmed. In the experiments, photosensitizers of the second generation were used: «Photoditazine», the active substance of which is dimeglumin chloride E6, and «Dimegin», which includes a derivative of protoporphyrin IX-2,4-di(1-methoxyethyl)- deuteroporphyrin IX disodium salt. In the described research work, the minimum concentrations of the studied drugs were determined by the method of sequential tenfold dilutions to obtain a bactericidal effect on strains of sanitary-indicative microorganisms Enterococcus faecalis, Staphylococcus aureus, Salmonella enteritidis.

Sign in / Sign up

Export Citation Format

Share Document