scholarly journals Antioxidant Activity of Entrapped alfa-tocopherol and Lutein in PLGA Nanoparticles in Wistar Rats

2019 ◽  
Vol 56 (3) ◽  
pp. 520-534
Author(s):  
Daniela Miricescu ◽  
Alexandra Totan ◽  
Iulia Ioana Stanescu ◽  
Andreea Didilescu ◽  
Ruxandra Sfeatcu ◽  
...  
Keyword(s):  
Vitamin E ◽  
Wistar Rats ◽  
Plga Nanoparticles ◽  
Water Soluble ◽  
Control Group ◽  
Daily Dose ◽  
Total Antioxidant ◽  
Poorly Water Soluble ◽  
Hypercaloric Diet ◽  
Hepatic Level

The hypercaloric diet leads to obesity which is a risk factor for developing many systemic diseases such as diabetes, cardiovascular diseases and cancer. The purpose of our study was to observe the antioxidants effects of entrapped alfa-tocopherol (vitamin E) and lutein in Poly Lactic-CO-Glycolic acid (PLGA) in Wistar rats that received 3 weeks hypercaloric diet. Hepatic and splenic lysates have been used to detect changes in total antioxidant capacity (TAC) and advanced oxidative protein products (AOPP). The AOPP liver levels were statistically decreased in the case of rats groups that received a daily dose of PLGA-lutein or PLGA-vitamin E versus the control group. The AOPP level was decreased but statistically insignificant in Wistar rats spleen who received a daily dose of PLGA-lutein. At hepatic level, TAC was statistically increased in rats groups that received a daily dose of vitamin E or lutein. An increased statistical level of TAC was observed in the spleen cell lysing in Wistar rats who received the daily dose of PLGA-vitamin E. Entrapment of vitamin E and lutein in PLGA structure has diminished the effects of hypercaloric diet and can be considered a attractive and promising approach to enhance the bioavailability and activity of poorly water soluble compounds.

scholarly journals The Antioxidant Effects of PLGA-based Nanoparticles Loaded with Vitamin E in Rats Treated with Hypercaloric Diet

2019 ◽  
Vol 56 (2) ◽  
pp. 337-340
Author(s):  
Daniela Miricescu ◽  
Daniela Gabriela Balan ◽  
Catalina Radulescu ◽  
Radu Radulescu ◽  
Iulia-Ioana Stanescu ◽  
...  
Keyword(s):  
Vitamin E ◽  
Drug Delivery Systems ◽  
Wistar Rats ◽  
Male Rats ◽  
Control Group ◽  
Daily Dose ◽  
Hypercaloric Diet ◽  
Antioxidant Effects ◽  
Wistar Male Rats ◽  
Biomarkers Of Oxidative Stress

PLGA (Poli-lactic-co-glycolic acid) nanoparticles (NPs) are curently used as drug delivery systems for many types of drugs including antioxidants such as vitamin E. The main aim of our study was to test the antioxidant effects of PLGA-vitamin E on Wistar male rats. Two groups of Wistar rats received a hypercaloric diet for 21 days: the first group received besides the hypercaloric diet a daily dose of PLGA loaded with vitamin E and the control group received only the hypercaloric diet. Spleen cellular lysate has been used to detect biomarkers of oxidative stress such as malondialdehyde, glutathione, advanced human oxidative protein and vitamin E. After 3 weeks of treatment, statistical significant changes have been detected between the two groups.

scholarly journals Hypercaloric Diet and Vitamin E Intake

2019 ◽  
Vol 56 (4) ◽  
pp. 937-941
Author(s):  
Catalina Radulescu ◽  
Daniela Miricescu ◽  
Alexandra Totan ◽  
Andreea Cristina Didilescu ◽  
Iulia-Ioana Stanescu ◽  
...  
Keyword(s):  
Vitamin E ◽  
Wistar Rats ◽  
Glycolic Acid ◽  
Redox Status ◽  
Plga Nanoparticles ◽  
Antioxidant Effect ◽  
Daily Dose ◽  
Group 2 ◽  
Hypercaloric Diet ◽  
Group 1

PLGA (poly-lactic-co-glycolic acid) nanoparticles represent an important synthetic biocomponent that has the potential to be a promising carrier of drugs, proteins, nucleic acids, due to its biodegradability and minimal side effects. The aim of our study was to observe the antioxidant effect of vitamin E loaded in PLGA nanoparticles administered over a period of 3 weeks in Wistar rats treated with a hypercaloric diet. Glutathione (GSH) and malondiadehyde (MDA) biomarkers determined from liver lysate were analyzed to evaluate the oxidative stress (OS) induced by the hypercaloric diet. The results of our study revealed a statistically significant increase for GSH and vitamin E in group 2 of Wistar rats receiving hypercaloric diet and a daily dose of vitamin E versus group 1 (p[0.005). The antioxidant effect of vitamin E was also observed by the statistically significant decrease of MDA in group 2 of Wistar rats compared with group 1. The daily dose of vitamin E has improved the liver redox status of group 2 Wistar rats.

Assessment of Labrasol®/Labrafil®/Transcutol® (4/4/2, v/v/v) as a non-clinical vehicle for poorly water-soluble compounds after 4-week oral toxicity study in Wistar rats

2010 ◽  
Vol 57 (2-3) ◽  
pp. 284-290 ◽  
Author(s):  
J.-L. Delongeas ◽  
G. Vermeil de Conchard ◽  
A. Beamonte ◽  
H. Bertheux ◽  
C. Spire ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Water Soluble ◽  
Toxicity Study ◽  
Oral Toxicity ◽  
Poorly Water Soluble

Probable antioxidant therapy of Saffron Crocin in patients with multiple sclerosis: A randomized controlled trial

Biomedicine ◽  
2021 ◽  
Vol 40 (4) ◽  
pp. 516-521
Author(s):  
Sara Ami Ahmadi ◽  
Azin Kazemi ◽  
Mohammadmahdi Sabahi ◽  
Shahab Razipour ◽  
Arash Salehipour ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Multiple Sclerosis ◽  
Controlled Trial ◽  
Stress Factors ◽  
Control Group ◽  
Oxidative Damages ◽  
Significant Difference ◽  
Daily Dose ◽  
Total Antioxidant ◽  
And Control

Introduction and Aim: Multiple Multiple sclerosis (MS) is a complex neurological condition  might emerge as a result ofcomplex combination of genetic risk factors with environmental triggers, including oxidative stress. in this study we aimed to evaluate the effects of oral Crocin on oxidative stress in patients with MS.  Materials and Methods: Adjunct to standard treatment, the Crocin group (20 patients) received 30-mg/day (15 mg twice daily) dose of Crocin and placebo group (20 patients) received for 4 weeks. Saliva and urine samples were collected to determine the levels of total antioxidant capacity (TAC), catalase activity (CAT), total thiol groups (TTG), lipid peroxidation (LPO), were measured at baseline and the end of the study.   Results: At baseline, there were no significant differences of LPO, TAC, CAT, and TTG of urine between the control and case groups. However, a significant difference was found after 4 weeks of Crocin-therapy in TTG,TAC and LPO (p<0.05) except in CAT activity (P>0.05). We found no deffrence in urinary TTG level and CAT activity in control group at the end of intervention (P>0.05), while TAC and LPO level were significantly different at the end of the study as compared with the beginning (P<0.05). Althugh, we found no significant difference in saliva LPO, TTG and TAC levels and the activity of CAT in case and control groups at first (p>0.05), Crocin administration have resulted in a significant increase in saliva TTG and TAC levels as well as CAT activity and markedly decrease in LPO level (p<0.05). Conclusion: According to the results of this study, Crocin can significantly reduce the several oxidative stress factors in MS patients and may contributes to attenuates the oxidative damages.

scholarly journals Gebe Ratlara uygulanan Akrilamid ve E Vitaminin Plasenta Dokusu Üzerine Olası Etkilerinin Araştırılması

2017 ◽  
Vol 5 (4) ◽  
pp. 349
Author(s):  
Mehmet Erman Erdemli ◽  
Eyüp Altınöz ◽  
Zeynep Aksungur ◽  
Zümrüt Doğan ◽  
Harika Gözükara Bağ ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Total Antioxidant Capacity ◽  
Corn Oil ◽  
Reduced Glutathione ◽  
Control Group ◽  
Vaginal Smear ◽  
Protective Agent ◽  
Pregnant Rats ◽  
Total Antioxidant

Investigate the changes that occur in the placenta tissues of pregnant rats that were administered acrylamide (AA) and vitamin E as a protective agent during pregnancy. Thirty rats that were proven positive for pregnancy with vaginal smear test were randomly distributed into control, corn oil, vitamin E, acrylamide and vitamin E + acrylamide groups. Pregnant rats were decapitated on the 20th day of the experiment. Malondialdehyde (MDA), reduced glutathione (GSH), total antioxidant capacity (TAS), total oxidant capacity (TOS) and Xanthine oxidase (XO) levels were measured in placenta tissues. It was determined that acrylamide application during pregnancy statistically significantly increased MDA, TOS and XO levels and reduced GSH and TAS levels in the placenta tissue of pregnant rats when compared to all other groups, and GAS and TAS levels statistically significantly increased in vitamin E administered group when compared to all other groups and TOS and XO levels were decreased to control group levels. It was observed that orally administered AA changed the antioxidant / oxidant equilibrium favoring the oxidants by increasing MDA, XO and TOS levels in pregnant rats and caused oxidative stress, while vitamin E administration returned the antioxidant / oxidant equilibrium back to normal levels, preventing oxidative stress induced toxicity.

scholarly journals Evaluation of the toxic influence of vitamin E (dl-alpha-tocopheryl acetate) and treatment with aqueous extracts of cinnamon or anise on lipid profile and liver functions of female wistar rats

2017 ◽  
Vol 5 (2) ◽  
pp. 248
Author(s):  
Sabah Ibrahim ◽  
Murwan Sabahelkhier
Keyword(s):  
Vitamin E ◽  
Wistar Rats ◽  
Low Dose ◽  
Density Lipoprotein ◽  
Control Group ◽  
High Dose ◽  
Toxic Dose ◽  
Liver Functions ◽  
Female Wistar Rats ◽  
Better Than

The toxic effects of vitamin E and its treatment with aqueous extracts of Cinnamon or anise on lipid profile and liver functions of female wistar rats were examined for six weeks during September 2016 at labs in al-Neelain University. 18 rats were divided into six groups: 1. negative control group (sunflower oil), 2. Positive control group and rest groups given (1500 mg/Kg/BW/day of Vitamin E). After two hours, the four treated groups received a low dose (2.13g/Kg) and a high dose (3.20g/Kg) 20g/Kg from Cinnamon aqueous extract (CAE) and Anise aqueous extract (AAE). At the end rats were sacrificed then serum and liver tissues were analyzed. Vitamin E toxic dose had caused a significant increase in serum Triglycerides (TG), Alanine amino transferase (ALT) levels, while it decreased the levels of High-density lipoprotein (HDL), low-density lipoprotein (LDL), total cholesterol (TC) and Aspartate amino transferase (AST). All treatments decreased TG and ALT levels. CAE low dose significantly increased TC, LDL and HDL levels. CAE high dose caused a significant decrease in AST, TC, and LDL. Both doses of AAE, caused significant increases on AST levels, and only anise low dose caused a significant decrease on TC and LDL levels. Vitamin E toxic dose caused severe fatty change in liver histology, which was near normal in both doses of CAE with a small necrosis in a low dose. Only AAE low dose normalized the liver. To conclude Vitamin E oral administration with a dose of (1500 mg/Kg) induced liver injury with an elevation in ALT and TG levels, which was significantly ameliorated by both treatments. Cinnamon was better than anise in ameliorating the toxicity. Cinnamon high dose was better than Cinnamon low dose; in contrast anise low dose was better than Anise high dose.

Exploring the systemic delivery of a poorly water-soluble model drug to the retina using PLGA nanoparticles

2021 ◽  
pp. 105905
Author(s):  
Enqi Zhang ◽  
Nadezhda Osipova ◽  
Maxim Sokolov ◽  
Olga Maksimenko ◽  
Aleksey Semyonkin ◽  
...  
Keyword(s):  
Plga Nanoparticles ◽  
Water Soluble ◽  
Systemic Delivery ◽  
Soluble Model ◽  
Poorly Water Soluble ◽  
Model Drug

scholarly journals Sesame Meal, Vitamin E and Selenium Influence Goats’ Antioxidant Status

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 392
Author(s):  
Eleni Tsiplakou ◽  
Christina Mitsiopoulou ◽  
Chrysoula Karaiskou ◽  
Marica Simoni ◽  
Athanasios C. Pappas ◽  
...  
Keyword(s):  
Vitamin E ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Oxidative Status ◽  
Protein Carbonyls ◽  
Control Group ◽  
Sesame Meal ◽  
Total Antioxidant ◽  
Mda Content ◽  
The Impact

This study aimed to determine the impact of sesame meal, selenium (Se), and vitamin E (VitE) on goats’ oxidative status. Thirty mid-lactation crossbred goats were divided into five homogeneous groups, and were fed 1 kg of alfalfa hay and 1.2 kg of concentrates daily. The control group (C) received a basal diet. In the concentrates of the treated groups, 10% of the soybean meal was replaced by sesame meal and no extra VitE or Se (SM), or an extra 60 mg of VitE (SME), or 0.1 mg organic Se (SMSe), or their combination (60 mg VitE and 0,1 mg organic Se/kg of concentrate (SMESe). In the plasma of the goats, the dietary treatments did not affect glutathione reductase, glutathione peroxidase, glutathione transferase, catalase, superoxide dismutase activities, malondialdehyde (MDA) content, or the total antioxidant capacity. A reduction and a trend for lower protein carbonyls content was found in goats fed SM (p = 0.03) and SME (p = 0.06) compared to SMESe. In the milk, the lactoperoxidase activity decreased with SMSe and SMESe. A numerical decrease in the total antioxidant capacity and an increase in the MDA content in the milk of the SMESe group compared with the other treated groups was found. In mid-lactation goats, SM improves the oxidative status of both the organism and the milk.

scholarly journals Determination of oxidative stress parameters in fluoxetine users

2020 ◽  
Vol 8 (5) ◽  
pp. 172-182
Author(s):  
Magda Susana Perassolo ◽  
Juliana Raquel Raach ◽  
Tainara Gomes Vargas ◽  
Andressa Schmidt dos Santos ◽  
Natália Alves Silva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Major Depression ◽  
Control Group ◽  
Depression Treatment ◽  
First Line ◽  
Antioxidant Power ◽  
Oxidative Stress Parameters ◽  
Daily Dose ◽  
Total Antioxidant

Fluoxetine (FLU), a selective serotonin reuptake inhibitor, is the first line in depression treatment and it is involved in oxidative stress (OE). Thus, this study aimed to analyze the OE parameters in patients diagnosed with depression and treated with FLU. Were evaluated 121 volunteers divided into two groups: 58 fluoxetine users (with major depression) and 63 non-fluoxetine users (control group, without major depression). The OE was evaluated by determining the levels of malondialdehyde (MDA), total antioxidant power (FRAP) and activity of antioxidant enzymes glutathione peroxidase (GPx) and superoxide dismutase (SOD). MDA, FRAP, GPx and SOD were dosed in plasma. The influence of age, smoking, alcoholism, comorbidities, use of another drugs and antioxidants in the OE were evaluated. The results were compared between the groups. In relation to the fluoxetine daily dose, MDA presented higher levels in patients using 20 mg daily FLU when compared to the control group, as well as the activity of the GPx enzyme and the FRAP levels. In this way, the use of fluoxetine may interfere with the OE parameters, causing an increase in OE levels.

Antioxidative Role of Azadirachta Indica Vit. E in Restoration of Histopathological changes in Acetaminophen induced liver

2021 ◽  
Vol 15 (12) ◽  
pp. 3211-3213
Author(s):  
Jawad Mumtaz Sodha ◽  
Aftab Abbasi ◽  
Hina Mawani ◽  
Ghulam Nabi Pathan ◽  
Umair Ali Soomro ◽  
...  
Keyword(s):  
Vitamin E ◽  
Azadirachta Indica ◽  
Wistar Rats ◽  
Histopathological Examination ◽  
Control Group ◽  
Normal Appearance ◽  
Neem Extract ◽  
Hepatoprotective Effects ◽  
Group B ◽  
Group D

Aim: To study the comparative effect of Acetaminophen induced liver toxicity with aqueous extract of Azadirachta Indica (Neem) and vitamin E on the basis of liver Histopathology. Methods: Sixty Wistar Rats of both sexes were split into four groups. Each group contained 15 animals. The control group was group A, Group B was treated orally with single dose of Paracetamol 2 mg / kg by weight, Group C was administrated orally with aqueous Neem extract 500 mg/kg +2 mg/Kg by weight oral Paracetamol, and Group D was given orally paracetamol+Neem extract+Vitamin E with 100mg/Kg/body weight for 15 days. Rats from all groups were decapitated, the liver was sliced, and liver tissues were taken for histological examination. Tissue samples were fixed in 10% formaldehyde, embedding in paraffin followed by Hematoxylin and Eosin dye (H&E) and observed under 400x magnification with a digital microscope. Results: On Histopathological examination of the rat`s liver in we found that the control group had a normal appearance, colour, and uniform surface without any necrosis. Group B showed severe necrosis and haemorrhagic patches. In comparison, Group C revealed normal appearance, colour, and smooth surface with no necrotic alterations. Livers from the group D looked virtually normal in terms of colour, undersurfaces, and organ weight. However, hepatoprotective effects were observed in the Group C and D. Therefore, we can conclude that Azadirachta indica and Vitamin E could serve as a good medication for defence against liver injury. Conclusion: Our findings showed thatextract of Azadirachta Indica and Vitamin E exhibited hepatoprotective effects on the Wistar rats that were subjected to Acetaminophen. Key words: Azadirachta Indica leaf extract, Vitamin E, hepatoprotective Paracetamol, Wistar rats.

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