Clinical-statistical Study on the Biomimetic Adhesion of Whole Ceramic Inlays

Currently, a new approach to restorative dentistry is possible, from biomimetic point of view, by using restorative materials with a natural tooth-like structure and very strong adhesion to the hard remaining tissues. The objective of the study was to compare in vitro the marginal adaptation of restorations with whole ceramic inlays, using the biomimetic method compared to the classical method. A batch of 60 extracted impacted molars was used for the study. Large cavities were prepared at occlusal proximal surfaces according to minimally invasive therapy principles. The teeth were divided into 4 study groups (A, B, C, D). Batches A and C contain teeth prepared and restored through classical method, with entirely ceramic inlays. Batches B and D contain teeth restored through biomimetic adhesive method. After preparation, fluid composite (Gradia Direct Flo - GC) was used as a basic filling material to seal dentine wounds and dental canaliculi according to biomimetic principles. Cementing was done with Variolink Esthetic DC-Ivoclar (lot A and B) and with Maxcem Elite - Kerr (lot C and D). Samples were cut and prepared for microscopic analysis. The analysis of the four batches revealed the existence of the microfissure in the dentine wounds and the presence of fragments detached from the cementing material layer. The hybrid layer is homogeneous with qualitative dental canaliculi sealing. The difference between the two methods is the size of these defects, in the case of the classical method being approximately 2 times larger. The difference between the two types of cementing material used is due to the fact that in case of Maxcem Elite - Kerr cement, discontinuities have been observed at the level of cementing material - inlay material interface. The biomimetic method is superior to the classical one, the integrity of the layers of materials used in the biomimetic treatment is clearly superior to the integrity of the layers of material used in the classical treatment.

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Dissolution capacity of orange and eucalyptus oils versus endodontic types of cement (in vitro study)

Mexican Journal of Medical Research ◽

10.29057/mjmr.v10i19.7217 ◽

2022 ◽

Vol 10 (19) ◽

pp. 29-33

Author(s):

Sebastián M. Ulloa-Alvarez ◽

María E. Guerrero-Coello ◽

José E. Torracchi-Carrasco

Keyword(s):

In Vitro Study ◽

Control Group ◽

Filling Material ◽

Cement Sample ◽

Orange Oil ◽

Eucalyptus Oil ◽

Before And After ◽

The Difference ◽

Post Hoc

Introduction: Endodontic retreatment seeks reentry to the root canal system to remove all filling material, identification and correction of pathological or iatrogenic defects. Objective: This study aimed to compare the dissolving capacity of eucalyptus oil, orange oil, and distilled water versus three types of endodontic cements. Materials and methods: Two hundred and four stainless steel molds were fabricated, where each cement sample was placed to the edge and left to set in the incubator, simulating normal oral conditions for 7 days. Subsequently, the solubility of each cement was analysed by immersing the samples in eucalyptus and orange oil for ten minutes in a static and ultrasonic environment. The solubility of the cement was evidenced by the difference between the weight before and after contact with the oils, data were statistically analysed with the ANOVA test and the post hoc Tukey bootstrap means test with 1,000 repetitions. Results: The control group and eucalyptus oil having no significant effect (p>0.05), the ultrasonic environment was found to be effective (p<0.05), whereas orange oil had better dissolution effects (p=0.004). Conclusions: The most susceptible cement was Sealapex, while the least soluble was MTA-Fillapex in all the solvents studied.

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Infiltration of Rhodamin B into three materials used as cervical barrier

RGO - Revista Gaúcha de Odontologia ◽

10.1590/1981-8637201400030000022677 ◽

2014 ◽

Vol 62 (3) ◽

pp. 235-242

Author(s):

Grazielle Morais de SOUZA ◽

Luis Cardoso RASQUIN ◽

Fabíola Bastos de CARVALHO

Keyword(s):

Study Groups ◽

Control Group ◽

Sodium Perborate ◽

Filling Material ◽

Significant Difference ◽

Materials Used ◽

Level Of Significance ◽

Material Setting ◽

Rhodamin B

OBJECTIVE: To evaluate in vitro the sealing ability of three materials used as cervical barrier for internal dental bleaching. METHODS: Thirty-three canines were selected, and after biomechanical root canal preparation, the canals were filled by lateral condensation technique. After the filling material setting reaction, the teeth were randomly divided into three groups of ten teeth each, according to the following materials for constructing the cervical barrier: X Temp LC; Vitro Fil; Coltosol. The control group consisted of three teeth, without placement of the cervical cap. Sodium perborate /distilled water associated with 2% Rhodamin B were used as bleaching substance. After placing the bleaching agent in the pulp chamber, access cavities were sealed with temporary cement IRM and samples were stored at 37 ° for 7 days. After this period, the samples were cleaved longitudinally, and leakage was evaluated by the visual method. The data were submitted to Pearson test with a 5% level of significance. RESULTS: No statistically significant difference was found between the study groups, however, the largest number of specimens with a lower degree of leakage were found in the Coltosol group. CONCLUSION: All the materials evaluated for making the cervical cap allowed some level of leakage.

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Electrotonic Structure of Motoneurons in the Spinal Cord of the Turtle: Inferences for the Mechanisms of Bistability

Journal of Neurophysiology ◽

10.1152/jn.2001.85.1.391 ◽

2001 ◽

Vol 85 (1) ◽

pp. 391-398 ◽

Cited By ~ 28

Author(s):

Gytis Svirskis ◽

Aron Gutman ◽

Jørn Hounsgaard

Keyword(s):

Spinal Cord ◽

Classical Method ◽

Current Injection ◽

Membrane Properties ◽

Resting Membrane Potential ◽

Synaptic Membrane ◽

Field Stimulation ◽

The Difference ◽

Dendritic Branches

Understanding how voltage-regulated channels and synaptic membrane conductances contribute to response properties of neurons requires reliable knowledge of the electrotonic structure of dendritic trees. A novel method based on weak DC field stimulation and the classical method based on current injection were used to obtain two independent estimates of the electrotonic structure of motoneurons in an in vitro preparation of the turtle spinal cord. DC field stimulation was also used to ensure that the passive membrane properties near the resting membrane potential were homogeneous. In two cells, the difference in electrotonic lengths estimated with the two methods in the same cell was 6 and 9%. The majority of dendritic branches terminated at a distance of 1 electrotonic unit from the recording site. The longest branches reached 2 λ. In the third cell, the difference was 36%, demonstrating the need to use both methods, field stimulation and current injection, for reliable measurements of the electrotonical structure. Models of the reconstructed cells endowed with voltage-dependent conductances were used to explore generation mechanisms for the experimentally observed hysteresis in input current-voltage relation of bistable motoneurons. The results of modeling suggest that only some dendrites need to possess L-type calcium current to explain the hysteresis observed experimentally and that dendritic branches with different electrotonical lengths can be bistable. Independent bistable behavior in individual dendritic branches can make motoneurons complex processing units.

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On the Role of Transferrin in 67Ga Uptake by Tumor Cells

Nuklearmedizin ◽

10.1055/s-0038-1629447 ◽

1988 ◽

Vol 27 (04) ◽

pp. 151-153

Author(s):

P. Thouvenot ◽

F. Brunotte ◽

J. Robert ◽

L. J. Anghileri

Keyword(s):

Specific Binding ◽

Subcellular Fractionation ◽

Animal Blood ◽

Tumor Bearing ◽

Cell Structures ◽

The Difference ◽

Sarcoma Cells ◽

In Vitro Uptake

In vitro uptake of 67Ga-citrate and 59Fe-citrate by DS sarcoma cells in the presence of tumor-bearing animal blood plasma showed a dramatic inhibition of both 67Ga and 59Fe uptakes: about ii/io of 67Ga and 1/5o of the 59Fe are taken up by the cells. Subcellular fractionation appears to indicate no specific binding to cell structures, and the difference of binding seems to be related to the transferrin chelation and transmembrane transport differences

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Enhancement of ADP-induced Platelet Aggregation by Adrenaline in Vivo and Its Prevention

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647788 ◽

1973 ◽

Vol 29 (02) ◽

pp. 490-498 ◽

Cited By ~ 6

Author(s):

Hiroh Yamazaki ◽

Itsuro Kobayashi ◽

Tadahiro Sano ◽

Takio Shimamoto

Keyword(s):

Platelet Count ◽

Platelet Aggregation ◽

Platelet Rich Plasma ◽

The Other ◽

Endothelial Surface ◽

Important Interaction ◽

Blood Coagulability ◽

The Difference

SummaryThe authors previously reported a transient decrease in adhesive platelet count and an enhancement of blood coagulability after administration of a small amount of adrenaline (0.1-1 µg per Kg, i. v.) in man and rabbit. In such circumstances, the sensitivity of platelets to aggregation induced by ADP was studied by an optical density method. Five minutes after i. v. injection of 1 µg per Kg of adrenaline in 10 rabbits, intensity of platelet aggregation increased to 115.1 ± 4.9% (mean ± S. E.) by 10∼5 molar, 121.8 ± 7.8% by 3 × 10-6 molar and 129.4 ± 12.8% of the value before the injection by 10”6 molar ADP. The difference was statistically significant (P<0.01-0.05). The above change was not observed in each group of rabbits injected with saline, 1 µg per Kg of 1-noradrenaline or 0.1 and 10 µg per Kg of adrenaline. Also, it was prevented by oral administration of 10 mg per Kg of phenoxybenzamine or propranolol or aspirin or pyridinolcarbamate 3 hours before the challenge. On the other hand, the enhancement of ADP-induced platelet aggregation was not observed in vitro, when 10-5 or 3 × 10-6 molar and 129.4 ± 12.8% of the value before 10∼6 molar ADP was added to citrated platelet rich plasma (CPRP) of rabbit after incubation at 37°C for 30 second with 0.01, 0.1, 1, 10 or 100 µg per ml of adrenaline or noradrenaline. These results suggest an important interaction between endothelial surface and platelets in connection with the enhancement of ADP-induced platelet aggregation by adrenaline in vivo.

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Reliability of a Single β-Thromboglobulin Measurement in a Diabetic Population : Importance of PGE1 in Anticoagulant Mixture

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651094 ◽

1987 ◽

Vol 57 (02) ◽

pp. 201-204 ◽

Cited By ~ 2

Author(s):

P Y Scarabin ◽

L Strain ◽

C A Ludlam ◽

J Jones ◽

E M Kohner

Keyword(s):

In Vitro Release ◽

Mean Value ◽

Reliable Estimate ◽

Diabetic Patients ◽

Single Measurement ◽

Diabetic Population ◽

The Difference ◽

Vitro Release

SummaryDuring the collection of samples for plasma β-thromboglobulin (β-TG) determination, it is well established that artificially high values can be observed due to in-vitro release. To estimate the reliability of a single β-TG measurement, blood samples were collected simultaneously from both arms on two separate occasions in 56 diabetic patients selected for a clinical trial. From each arm, blood was taken into two tubes containing an anticoagulant mixture with (tube A) and without (tube B) PGE!. The overall mean value of B-TG in tube B was 1.14 times higher than in tube A (p <0.01). The markedly large between-arms variation accounted for the most part of within-subject variation in both tubes and was significantly greater in tube B than in tube A. Based on the difference between B-TG values from both arms, the number of subjects with artifically high B-TG values was significantly higher in tube B than in tube A on each occasion (overall rate: 28% and 14% respectively). Estimate of between-occasions variation showed that B-TG levels were relatively stable for each subject between two occasions in each tube. It is concluded that the use of PGEi decreases falsely high B-TG levels, but a single measurement of B-TG does not provide a reliable estimate of the true B-TG value in vivo.

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EKSTRAK KULIT BUAH MANGGIS (Garcinia Mangostana L.) SEBAGAI OBAT ANTI DIARE

Jurnal Penelitian Farmasi & Herbal ◽

10.36656/jpfh.v2i1.195 ◽

2019 ◽

Vol 2 (1) ◽

pp. 9-13

Author(s):

Zaim Anshari ◽

Chrismis Novalinda Ginting ◽

Linda Chiuman ◽

Yuliani Mardiati Lubis

Keyword(s):

Blood Vessels ◽

Smooth Muscles ◽

Latin Square ◽

Ethanol Extract ◽

Garcinia Mangostana ◽

Pharmacology And Toxicology ◽

The Difference ◽

North Sumatra ◽

Papaverine Hydrochloride

This study aims to determine whether mangosteen rind extract (in the form of ethanol extract/EE) can be used as an anti-diarrhea drug after compared with other anti-diarrhea substances in three experimental groups. This research is an in vitro experimental study using adult male guinea pigs weighing 400-600 gr through the standard method of Magnus with the Latin square controlled experiment design. The study was conducted at the Pharmacology and Toxicology Laboratory of the Faculty of Pharmacy, University of North Sumatra. The results showed that the contraction of ileum in Ach with Atp + Ach compared the difference in contraction of ileum Ach with EE + Ach showed the difference in difference between the two contractions of the ileum was significant, the contraction of ileum in His with Dip + His compared indifference in contraction of ileum His with EE + His showed a difference indifference. the two ileal contractions are significant, the ileal contraction in the bar with Papa + Bar compared to the difference between the ileum bar contraction with EE + Bar shows no difference in the difference between the two ileum contractions. The conclusion is that the Mangosteen Skin Ethanol Extract works similarly to Papaverine Hydrochloride which is an antidiarrheal drug used to relax smooth muscles so that it can also make blood vessels dilate by relaxing smooth muscles in the walls of blood vessels.

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Efficacy of loading dose colistin versus carbapenems for treatment of extended spectrum beta lactamase producing Enterobacteriaceae

Scientific Reports ◽

10.1038/s41598-020-78098-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Wasan Katip ◽

Jukapun Yoodee ◽

Suriyon Uitrakul ◽

Peninnah Oberdorfer

Keyword(s):

Clinical Response ◽

Clinical Information ◽

Resistant Bacteria ◽

Loading Dose ◽

Extended Spectrum Beta Lactamase ◽

Clinical Utilization ◽

The Difference ◽

Infection Types ◽

Intra Abdominal Infection

AbstractColistin provides in vitro activity against numerous ESBL-producing and carbapenem-resistant bacteria. However, clinical information with respect to its utilization in infection caused by ESBL producers is limited. The aim of this study was a comparison of mortality rates of loading dose (LD) colistin and carbapenems as definitive therapies in a cohort of patients with infections caused by ESBL-producing Escherichia coli and Klebsiella pneumoniae. A retrospective cohort study in 396 patients with ESBL-producing E.coli and K.pneumoniae infection at a university-affiliated hospital was conducted between 1 January 2005 and 30 June 2015 to compare outcomes of infected patients who received LD colistin (95 patients) with carbapenems (301 patients). The three primary outcomes were 30-day mortality, clinical response and microbiological response. The most common infection types were urinary tract infection (49.49%), followed by pneumonia (40.66%), bacteremia (13.64%), skin and soft tissue infections (4.80%) and intra-abdominal infection (3.03%). LD colistin group provided higher 30-day mortality when compared with carbapenems group (HR 7.97; 95% CI 3.68 to 17.25; P = 0.001). LD colistin was also independently associated with clinical failure (HR 4.30; 95% CI 1.93 to 9.57; P = 0.001) and bacteriological failure (HR 9.49; 95% CI 3.76 to 23.96; P = 0.001) when compared with those who received carbapenems. LD colistin treatment was associated with poorer outcomes, i.e. mortality rate, clinical response and microbiological response. Moreover, when adjusted confounding factors, LD colistin was still less effective than carbapenems. It should be noted that, however, the use of Vitek-2 to assess colistin susceptibility could provide inaccurate results. Also, the difference in baseline characteristics could still remain in retrospective study although compensation by hazard ratio adjustment was performed. Therefore, clinical utilization of LD colistin should be recommended as an alternative for treatment ESBL-producing Enterobacteriaceae only in the circumstances where carbapenems cannot be utilized, but this recommendation must be considered carefully.

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Comparison of Fracture Resistance in Thermal and Self-Curing Acrylic Resins—An In Vitro Study

Polymers ◽

10.3390/polym13081234 ◽

2021 ◽

Vol 13 (8) ◽

pp. 1234

Author(s):

António Sérgio Silva ◽

Aurora Carvalho ◽

Pedro Barreiros ◽

Juliana de Sá ◽

Carlos Aroso ◽

...

Keyword(s):

Fracture Resistance ◽

Research Work ◽

Testing Machine ◽

In Vitro Study ◽

Dimensional Accuracy ◽

Acrylic Resin ◽

Thermosetting Resins ◽

Acrylic Resins ◽

The Difference

Thermal and self-curing acrylic resins are frequently and versatilely used in dental medicine since they are biocompatible, have no flavor or odor, have satisfactory thermal qualities and polishing capacity, and are easy and fast. Thus, given their widespread use, their fracture resistance behavior is especially important. In this research work, we comparatively analyzed the fracture resistance capacity of thermo and self-curing acrylic resins in vitro. Materials and Methods: Five prosthesis bases were created for each of the following acrylic resins: Lucitone®, ProBase®, and Megacryl®, which were submitted to different forces through the use of the CS® Dental Testing Machine, usually mobilized in the context of fatigue tests. To this end, a point was defined in the center of the anterior edge of the aforementioned acrylic resin bases, for which the peak tended until a fracture occurred. Thermosetting resins were, on average, more resistant to fracture than self-curable resins, although the difference was not statistically significant. The thermosetting resins of the Lucitone® and Probase® brands demonstrated behavior that was more resistant to fracture than the self-curing homologues, although the difference was not statistically significant. Thermosetting resins tended to be, on average, more resistant to fracture and exhibited the maximum values for impact strength, compressive strength, tensile strength, hardness, and dimensional accuracy than self-curing resins, regardless of brand.

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Effect of Citric Acid on Color Changes of Calcium Silicate-Based Cements an In Vitro Study

Applied Sciences ◽

10.3390/app11052339 ◽

2021 ◽

Vol 11 (5) ◽

pp. 2339

Author(s):

Joanna Metlerska ◽

Till Dammaschke ◽

Mariusz Lipski ◽

Irini Fagogeni ◽

Anna Machoy-Mokrzyńska ◽

...

Keyword(s):

Citric Acid ◽

Calcium Silicate ◽

In Vitro Study ◽

Vitro Study ◽

Color Measurement ◽

Color Changes ◽

Naked Eye ◽

Root Canal Irrigants ◽

The Difference

The aim of the present in vitro study was to investigate the effects of 10% and 40% citric acid (CA) on the color of calcium silicate–based cements (CSCs) in comparison to the effects of common root canal irrigants. Samples of six CSCs (n = 6)—ProRoot MTA (Dentsply, Tulsa, OK, USA), Biodentine (Septodont, Saint-Maur-des-Fossés, France), MTA Plus (Avalon Biomed Inc, by Prevest Denpro Limited, Jammu, India), MTA Repair HP (Angelus, Londrina, PR, Brazil), Ortho MTA (BioMTA, Seoul, Korea), and Retro MTA (BioMTA, Seoul, Korea)—were immersed in 10% and 40% CA as well as 15% EDTA, 2% NaOCl, 2% CHX, and 0.9% NaCl for 15 min, 1 h, and 24 h. ΔE values, representing the difference between the final and baseline values of the color components, were then determined using a VITA Easyshade Compact 5.0 spectrophotometer. Naked-eye evaluation of the changes in color and structures of the materials was performed using our own scale. Upon immersion of the materials in both 10% and 40% CA, there were statistically significant differences between spectrophotometric color measurement results for all CSCs (P < 0.05). However, CA does not cause dark discoloration, observable with the naked eye, of any of the materials, such as NaOCl and CHX. Significant statistical differences were also found between all CSCs in terms of submersion duration (P < 0.05). CA, which could be an alternative to EDTA use, caused greater CSCs discoloration and changed some of their structures. Unless required by the therapeutic procedure, clinicians should pay attention to the fact that the irrigant may affect the CSCs discoloration and minimize the contact time of irrigant with CSCs.

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