scholarly journals Comparison of Garlic and Ginger Extract with Glibenclamide on Some Biochemical Standards in Diabetic Mice

2021 ◽  
pp. 1-6
Author(s):  
Kholoud Yousef ◽  
Hiam Fadel ◽  
Mofeed Yaseen
Keyword(s):  
Blood Serum ◽  
Total Cholesterol ◽  
Allium Sativum ◽  
Normal Values ◽  
Ethanolic Extract ◽  
Alcoholic Extract ◽  
Diabetic Mice ◽  
Male Mice ◽  
Physiological Control ◽  
Ginger Extract

This study aimed to determine the effect of ethanolic extract of garlic (Allium sativum s.) and ginger on the levels of glucose, cholesterol, and triglycerides in the blood serum of adult white mice with induced diabetes. This study included 40 male mice of Balb/c, which were divided into four experimental groups (ten mice per group). The results showed a significant increase (p<0.05) in the concentration of glucose, cholesterol and triglycerides in the blood serum of the group of mice injected with alloxan compared with the physiological control. The results also showed a significant decrease (p<0.05) in the concentration of glucose, cholesterol and triglycerides in the blood serum of the group of mice treated with garlic and ginger extract compared to control glucose. Results showed that ethanolic extract of garlic (Allium sativum s.) and ginger has an important effect on lowering the level of biochemical to normal values equivalent to glibenclamide. KEYWORDS Alcoholic extract, alloxan, glucose, balb/c total cholesterol, triglycerides

scholarly journals Effect of garlic and ginger extracts on the levels of glucose and Peptide-c in diabetic mice: تأثير مستخلصي الثوم والزنجبيل في مستوى سكر الدم والببتيد c عند الفئران المستحدث عندها داء السكري

2021 ◽  
Vol 5 (4) ◽  
pp. 119-105
Author(s):  
Hiam Kamel Fadil, Kholoud Mostafa Sheikh Yousef Hiam Kamel Fadil, Kholoud Mostafa Sheikh Yousef
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Concentration ◽  
Physiological Saline ◽  
Alcoholic Extract ◽  
Diabetic Mice ◽  
Physiological Control ◽  
Fourth Group ◽  
The Third ◽  
Hypoglycemic Agent

This study aimed to investigate the effect of the alcoholic extract of garlic and ginger together on the levels of glucose, peptide -c and body weight in diabetic white mice. The study included 40 male white mice, Balb/c strain, which were divided into four experimental groups (10 mice in each group). The first group was a physiological control that was injected with physiological saline (0.9%) until the end of the experiment. As for the second group, diabetes was induced with a dose of 200 mg/kg of Alloxan hydrate weight of the mouse only, while the third group developed diabetes, and then it was treated with alcoholic extract of garlic and ginger together at a dose of 500 mg/kg of mouse weight for 10 days. While the fourth group developed diabetes and was treated with Glibenclamide. At the end of the experiment, the animals were anesthetized and blood was drawn from them by cardiocentesis. The results showed the effectiveness of garlic and ginger extracts in reducing blood glucose concentration by 35.75% and returning Peptide-c levels to their normal levels, equivalent to Glibenclamide (glyburide), which is known as an oral hypoglycemic agent.

scholarly journals Comparative Evaluation of Polyherbal Combination for Hypolipidemic Activity

2009 ◽  
Vol 1 (01) ◽  
Author(s):  
Shrivastava R. ◽  
Solanki S. S. ◽  
Tomar V. ◽  
Garud N. ◽  
Garud A. ◽  
...  
Keyword(s):  
Serum Level ◽  
Total Cholesterol ◽  
Total Cholesterol Level ◽  
Curcuma Longa ◽  
Density Lipoprotein ◽  
Ethanolic Extract ◽  
Blood Cholesterol ◽  
Gymnema Sylvestre ◽  
Alcoholic Extract ◽  
Cholesterol Diet

Hyperlipidemia is defined as increase in the lipid content (group of fat or fat like substances along with their lipoprotein counterpart) in blood. The present study was undertaken to explore the antihyperlipidemic effect of ethanolic extract from Fenugreek and Gymnema sylvestre and hydro-alcoholic extract from Curcuma longa in cholesterol diet induced hyperlipidemia in rats. Oral administration of various extracts once in a day for two weeks significantly lowered the total cholesterol phospholipid, triglycerides, low density lipoproteins and increase the high density lipoprotein level. Present investigation shows that Curcumin caused more significant decrease in the total cholesterol level (1.36 %), serum level of triglyceride (1.32 %), and serum level of LDL (1.39 %) as compared to other groups in cholesterol diet induced hyperlipidemia and atherosclerosis. The study exhibited that elevated blood cholesterol; triglycerides, LDL, and decrease HDL which occur in Hyperlipidemia were significantly reduced by the administration of combination of Curcumin, Gymnema sylvestre and Fenugreek.

scholarly journals Physiological and histological effect of aqueous and alcoholic extract of Garlic (Allium sativum) on testicular function of albino male mice treated with lead acetate

2014 ◽  
Vol 8 (2) ◽  
pp. 41-48
Author(s):  
Salah M.M. AL-chalabi ◽  
Rashaa F. Abdul-Lattif ◽  
Dalia A. Sabrei
Keyword(s):  
Testosterone Level ◽  
Lead Acetate ◽  
Allium Sativum ◽  
Sperm Concentration ◽  
Control Group ◽  
Testicular Function ◽  
Alcoholic Extract ◽  
Male Mice ◽  
Abnormal Sperm ◽  
Histological Effect

This study was carried out to investigate the effect of oral administration of aqueous and alcoholic extract of Allium sativum (Garlic) on testicular function in male mice exposed to lead acetate. Sixty adult (7-8) weeks old male mice were divided into six groups (10 micegroup), first and second group were administered with 150mgkg body weight BWday of aqueous and alcoholic extract of plant respectively. However, the third group was treated with 100 mgkgBWday of lead acetate. While the fourth and fifth groups were administered with 150 mgkgBWday. Of aqueous and alcoholic extract combination with 100 gmkg. BW of lead acetate. The sixth group treated with distilled water and served as control group throughout five weeks. At the end of experiment, treated animals were sacrificed and sperm were collected from caudal epidydymis to use for following tests: sperm concentration, motility, deadalive sperm and morphological abnormalities. Serum was isolated to assay for the analysis of, FSH, LH and testosterone level. The results showed a significant increase in sperm concentration, motility and decrease in dead and abnormal sperm in the group treated with aqueous and alcoholic extract of Allium sativum. However, the results showed decrease in sperm concentration, motility and increase in dead and abnormal sperm in the group treated with lead acetate. The other groups of animals groups treated with aqueous and alcoholic extract combination with lead acetate showed improvement in sperm concentration and motility compared to that treated with lead acetate The FSH, LH and testosterone level significantly increased in treated groups with aqueous and alcoholic extract of plant compared to group treated with lead acetate. It's concluded that aqueous and alcoholic extract of Allium sativum were significantly improved fertility in treated medicine compared to animals treated with lead acetate.

Hypoglycemic effects of Auricularia auricula polysaccharides on high fat diet and streptozotocin-induced diabetic mice using metabolomics analysis

2021 ◽  
Author(s):  
nannan liu ◽  
Xuefeng Chen ◽  
Juanna Song ◽  
Mengyin Chen ◽  
Pin Gong ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
High Fat Diet ◽  
Diabetic Mice ◽  
Male Mice ◽  
High Fat ◽  
Auricularia Auricula ◽  
Ultrahigh Performance Liquid Chromatography ◽  
Metabolomic Approach ◽  
Metabolomics Analysis

This study evaluated the hypoglycemic effect of Auricularia auricula polysaccharides (AAPs) on streptozotocin-induced type 2 diabetes mellitus (T2DM) male mice (C57BL/6J) using a metabolomic approach based on ultrahigh-performance liquid chromatography–Q...

scholarly journals BIOCHEMICAL EVALUATION OF CHRONIC CONSUMPTION OF MONOSODIUM GLUTAMATE ON LIVER OF WISTAR ALBINO RATS

2021 ◽  
pp. 99-102
Author(s):  
SURENDRA BABU THANGACHI ◽  
VARSHA SRIRAM MOKHASI ◽  
SHABINA KOMATH CHENOLY
Keyword(s):  
Blood Serum ◽  
Total Cholesterol ◽  
Total Bilirubin ◽  
Liver Enzymes ◽  
Monosodium Glutamate ◽  
Density Lipoprotein ◽  
Lipid Profiles ◽  
Albino Rats ◽  
High Dose ◽  
Wistar Albino Rats

Objective: The objective of this study was to determine if there were any harmful effects of monosodium glutamate (MSG) on the liver of Wistar albino rats chronically at three different doses, namely, low, mid, and high doses equivalent to human consumption doses in developing countries. Methods: The Wistar albino rats (n=24) were divided into four groups, namely control, Low dose MSG (180 mg/kg), Mid dose MSG (360 mg/kg), and High dose MSG (720 mg/kg). At the end of the experimental period (120 days), animal blood was collected retro-orbitally to analyze the liver enzymes such as aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), Total protein, Albumin, and Total Bilirubin in blood serum. Lipid profiles, namely, Triglycerides, low-density lipoprotein (LDL), high-density lipoprotein (HDL), and Total cholesterol were subjected to analysis using blood serum. Results: Significant increase (p<0.05) in AST, ALT, ALP, and total bilirubin in serum of MSG induced low, mid, and high dose groups when compared to control group were recorded. There was a significant increase (p<0.05) in LDL, decrease in HDL, increase in total cholesterol and triglycerides of MSG-induced animal groups. Conclusion: The effects of MSG on serum liver enzymes and lipid profiles in this present animal study were not severely alarming even though the dosage was chronic which opens further discussion on the controversies revolving around MSG.

scholarly journals Ketoksikan Akut dari Ekstrak Etanolik Daun Jarak Pagar (Jatropa curcas) pada Mencit Jantan Galur Balb/C

2014 ◽  
Vol 15 (1) ◽  
pp. 52
Author(s):  
Hanif Nasiatul Baroroh ◽  
Eka Prasasti Nur Rachmani
Keyword(s):  
Acute Toxicity ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Male Mice ◽  
Animal Test ◽  
Group I ◽  
Liver And Kidney ◽  
Single Dosage ◽  
Histopathology Examination ◽  
Lung And Kidney

The acute toxicity of Jatropa curcas leaves on Balb/C male mice was studied in rats. This research aimed to determine acute toxicity, evaluate spectrum of toxic effect and mechanism that caused the death of animal test after administration of ethanolic extract of J. curcas leaves, single dosage orally on 24 hours observation. The research used male mice, which are divided into 5 groups. Group I was negative control with CMC-Na. Group II, III, IV, and V were given extract with dose of 1400 mg/kgBW, 2240 mg/kgBW, 3584 mg/kgBW and 5734 mg/kgBW, respectively. Evaluation of the toxic symptoms and death of animal test was done for 24 hours. If the animal test was died before 24 hours then it underwent surgery to take the heart, liver, lung, and kidney. In the end of the evaluation, all mice were killed to take the vital organs for histopathologic examination. No mortality was observed during study. The test resulted LD50 of ethanolic extract from J. curcas leaves using Balb/C male mice was 5734 mg/kg of BW. It was categorized as practically not toxic. Administration of the extract did not cause alterations of animal behaviours. Histopathology examination shows inflammation in lung, liver, and kidney after administration of the extract.

scholarly journals Drug abuse and serum nutritional biomarkers: A retrospective cohort study

2021 ◽  
Vol 25 (2) ◽  
pp. 227-236
Author(s):  
Alfonso Daniel Silva Ochoa ◽  
José Alejandro Valdevila Figueira ◽  
Rocío Valdevila Santiesteban ◽  
Diego Javier Estrella Almeida ◽  
Luz Maria Valencia Erazo ◽  
...  
Keyword(s):  
Drug Abuse ◽  
Blood Serum ◽  
Total Cholesterol ◽  
Retrospective Cohort ◽  
Nutritional Assessment ◽  
Fasting Glucose ◽  
Age Groups ◽  
Public Health Problem ◽  
Drug Addicts ◽  
Convenience Sample

Introduction: Drug abuse is a public health problem around the globe. Its implications in human health are harmful, compromising nutritional status. It has been shown that malnutrition is moderately prevalent in drug addicts, and a nutritional prescription is significantly beneficial for these patients. Available literature suggests altered blood serum biochemical data in drug addicts. Our study focused on blood serum nutritional biomarkers in drug addicts who did not have a nutritional assessment or treatment. This study aimed to analyze nutritional blood serum biomarkers in subjects diagnosed with drug addiction from January 2010 to June 2020.Methods: The research was a retrospective cohort, analytical, observational, and was based on a convenience sample. Data about blood serum AST, ALT, fasting glucose, urea, creatinine, total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides, and hemoglobin were analyzed from a database of 103 subjects diagnosed with mental and behavioral disorders due to the use of drugs and other psychoactive substances (ICD-10: F10-F19) in the Institute of Neurosciences (INC). Consumed drugs were alcohol, cocaine, amphetamines, MDNA, opioids, marijuana, and psychotropic drugs. Results: The medians of hemoglobin, total cholesterol, HDL, and creatinine statistically differed between genders and age groups. There were more cases of low blood hemoglobin and hyperglycemia levels in men, (20.4, and 8.7%, respectively) than women (4.9%, and 0%, respectively). There were low levels of fasting glucose in 8.8% of our sample. Serum creatinine levels were significantly increased in subjects aged 30 or more. Conclusions:In our sample, there were statistically different medians of hemoglobin, total cholesterol, HDL, and creatinine among groups of gender and age in drug addicts. All medians were within the normal range.

Direct Fluorometric Determination of Total Cholesterol in Serum Using Derivatized Cholesterol Oxidase

2000 ◽  
Vol 54 (8) ◽  
pp. 1157-1162 ◽  
Author(s):  
Javier Galbán ◽  
José F. Sierra ◽  
José M. López Sebastian ◽  
Susana de Marcos ◽  
Juan R. Castillo
Keyword(s):  
Blood Serum ◽  
Total Cholesterol ◽  
Cholesterol Oxidase ◽  
Enzymatic Reaction ◽  
Direct Determination ◽  
Analytical Signal ◽  
Cholesterol Concentration ◽  
Serum Samples ◽  
Response Range

In this paper the use of cholesterol oxidase derivatized with a fluorescein derivative is proposed for the direct determination of total cholesterol in blood serum. The method is based on the changes in the fluorescence of the solution during the enzymatic reaction (λexe = 498 nm and λem 519 nm). A mathematical model which relates the analytical signal to the total cholesterol concentration was developed, and the model can also be used to obtain some of the thermodynamic constants of the process. The method has a linear response range up to 70 mg/L of cholesterol, a detection limit of 2.5 mg/L, and the precision was 1.0% (40 mg/L cholesterol, n = 10). The method was applied to total cholesterol determination in blood serum samples. The results were compared to those obtained by a commercial analyzer, and statistically similar results were obtained. The use of derivatized cholesterol oxidase makes it possible to simplify the methodology normally used in this type of determination (the indicator reaction is avoided and the number of reagents reduced), with the added advantage that the analytical signal is independent of the concentrations of O2 and cholesterol oxidase.

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