scholarly journals Chemical analysis of some Ocimum basilicum medicinal teas

2021 ◽  
Vol 14 (3) ◽  
pp. 145-148
Author(s):  
Daniela BENEDEC ◽  
◽  
Diana ROZSA (RUS) ◽  
Daniela HANGANU ◽  
Ilioara ONIGA ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Phenolic Compounds ◽  
Caffeic Acid ◽  
Ocimum Basilicum ◽  
Caffeic Acid Derivatives ◽  
Aromatic Character ◽  
Aerial Parts ◽  
Three Samples ◽  
Therapeutic Properties ◽  
Layer Chromatography

Objectives. Ocimum basilicum is one of the widespread medicinal plants, widely used due to its multiple therapeutic properties (antimicrobial, antiviral, hypoglycemic, antispasmodic, anti-inflammatory, carminative, stomachic, antioxidant, analgesic, etc.), as well as its specific aromatic character. The aim of this study was to comparatively investigate the chemical composition (flavonoids, caffeic acid derivatives, tannins, essential oil) of the aerial parts of some Romanian medicinal teas of Ocimum basilicum. Materials and methods. The content of flavonoids, phenolic acids and tannins in three samples of O. basilicum tea were determined spectrophotometrically. Identification of important phenolic compounds (rutin, isoquercitrin chlorogenic acid and caffeic acid) was performed by thin layer chromatography (TLC). The essential oils were obtained by hydrodistillation method. Outcomes and conclusions. Following the quantitative determinations performed, it can be seen that the basil samples are rich in active principles, and their use for the treatment of some diseases can be justified.

scholarly journals Stem Lettuce and Its Metabolites: Does the Variety Make Any Difference?

Foods ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 59
Author(s):  
Janusz Malarz ◽  
Klaudia Michalska ◽  
Anna Stojakowska
Keyword(s):  
Chemical Composition ◽  
Plant Material ◽  
Methanolic Extract ◽  
Sesquiterpene Lactones ◽  
Scavenging Activity ◽  
Nmr Analysis ◽  
Caffeic Acid Derivatives ◽  
Aerial Parts ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

The objective of the present study was to characterize chemical composition of hitherto unexamined aerial parts of Lactuca sativa var. angustana cv. Grüner Stern. In contrast to leafy and head varieties of the lettuces, asparagus lettuce grown in Europe is much less studied. Fractionation of a methanolic extract from leaves of L. sativa cv. Grüner Stern, supported with HPLC/DAD and 1H NMR analysis, led to the isolation and/or identification of numerous terpenoid and phenolic compounds, including five apocarotenoids—(-)-loliolide, (+)-dehydrovomifoliol, blumenol A, (6S,9S)-vomifoliol, and corchoionoside C; three sesquiterpene lactones; two lignans—((+)-syringaresinol and its 4-O-β-glucoside); five caffeic acid derivatives; and three flavonoids. Some of the compounds, to the best of our knowledge, have never been isolated from L. sativa before. Moreover, monolignols, phenolic acids and a tryptophan-derived alkaloid were found in the analyzed plant material. Stems, leaves and shoot tips of the asparagus lettuce were examined to assess their phenolics and sesquiterpene lactone content as well as DPPH scavenging activity. Another stem lettuce—L. sativa var. angustana cv. Karola, two cultivars of leafy lettuces and one species of wild lettuce—L. serriola, were also examined as a reference material using HPLC/DAD. The results have been discussed regarding our previous studies and the literature data available.

scholarly journals Identification of phenolic compounds of dry extract of dandelion herb and large burdock leaf tea

2019 ◽  
Vol 18 (2) ◽  
pp. 73-77
Author(s):  
L. M. Fedoseeva ◽  
Yu. I. Chistova
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
High Performance ◽  
Uv Light ◽  
Spectral Characteristics ◽  
Water System ◽  
Dry Extract ◽  
Caffeic Acid Derivatives ◽  
Layer Chromatography

The purpose of this work is to study of phenolic compounds in the dry extract of dandelion herb and large burdock leaf tea.Materials and methods . The separation and identification of phenolic compounds of dry extract of dandelion herb and large burdock leaf tea by thin-layer chromatography and high-performance liquid chromatography with UV-detectionhas been carried out.Results . As a result of research, it has been established that during TLC the optimal system for the separation of phenolic compounds is the ethyl acetate – formic acid – water system (10:2:3). On the chromatogram four spots were found corresponding to the value of Rf and fluorescence in UV-light to flavonoids of the flavone group and phenolic acids (chlorogenic and caffeic acids). For further identification of phenolic compounds using HPLC, eight peaks were found, which in terms of retention time and spectral characteristics correspond to phenologlycosides, chlorogenic acid, caffeic acid derivatives, ferulic acid, umbelliferone.Conclusions . Thus, the dry extract of dandelion herb and large burdock leaf tea contains hydroxycinnamic acids and their derivatives, compounds of coumarin nature, phenologlycosides.

scholarly journals Natural Products from Scorzonera aristata (Asteraceae)

2010 ◽  
Vol 5 (5) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Manuela Jehle ◽  
Johanna Bano ◽  
Ernst P. Ellmerer ◽  
Christian Zidorn
Keyword(s):  
Natural Products ◽  
Methyl Ester ◽  
Chlorogenic Acid ◽  
Caffeic Acid ◽  
Acid Methyl Ester ◽  
Quinic Acid ◽  
The South ◽  
Caffeic Acid Derivatives ◽  
Aerial Parts ◽  
Acid Methyl

The aerial parts of Scorzonera aristata Ramond ex DC., collected in the South Tyrolean Dolomites, yielded the flavonoids quercetin 3- O-glucoside, rutin, and isoorientin, and the caffeic acid derivatives chlorogenic acid, 4,5-dicaffeoyl quinic acid, and 3,5-dicaffeoyl quinic acid. Sub-aerial parts contained caffeic acid methyl ester, 3,5-dicaffeoyl quinic acid, and the triterpenes 3α-hydroxyolean-5-ene, lupeol, and magnificol. Chemosystematic implications of the isolated compounds are discussed briefly.

scholarly journals Bioactive Phenolic Compounds from Aerial Parts of Plinia glomerata

2007 ◽  
Vol 62 (3-4) ◽  
pp. 196-200 ◽  
Author(s):  
Claúdia Serafin ◽  
Viviane Nart ◽  
Angela Malheiros ◽  
Márcia Maria de Souza ◽  
Luiz Fischer ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Spectroscopic Data ◽  
P Values ◽  
Analgesic Drugs ◽  
Antinociceptive Action ◽  
Chromatographic Techniques ◽  
Aerial Parts

Abstract The present work describes the antinociceptive properties and chemical composition of the aerial parts of Plinia glomerata (Myrtaceae). Both of the extracts evaluated, acetonic and methanolic, showed potent antinociceptive action, when analyzed against acetic acid-induced abdominal constrictions in mice, with calculated ID50 (mg/kg, i. p.) values of 24.8 and 3.3, respectively. Through usual chromatographic techniques with an acetonic extract, the following compounds were obtained: 3,4,3′-trimethoxy flavellagic acid (1), 3,4,3′-trimethoxy flavellagic acid 4′-O-glucoside (3) and quercitrin (4), which were identified based on spectroscopic data. Compounds 1 (ID50 = 3.9 mg/kg, i. p., or 10.8 μmol/kg) and 3 (ID50 = 1.3 mg/kg or 2.5 μmol/kg) were notably more active than some well-known analgesic drugs used here for comparison.

scholarly journals Pharmacognostical, Physicochemical and Preliminary Phytochemical Standardization of Aerial Parts of Onosma bracteatum Wall

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
Patel V. G. ◽  
Patel K. G. ◽  
Patel K. V. ◽  
Gandhi T. R.
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Microscopical Examination ◽  
Phytochemical Screening ◽  
Aerial Parts ◽  
Inorganic Acids ◽  
Physicochemical Evaluation ◽  
Thin Walled ◽  
Layer Chromatography

Objective: The present study was undertaken to develop the standardization parameters of powdered aerial parts of Onosma bracteatum Wall, family Boraginaceae. Methods: Different parameters such as pharmacognostical, physicochemical, preliminary phytochemical evaluation along with thin layer chromatography for identification of phytoconstituents were studied. Results: On microscopical examination of the aerial parts it showed the presence of oval to polygonal thin walled straight epidermal cells; spiral vessels, a few fibres elongated with blunt tips, long warty, tubercle based unicellular hairs and paracytic stomata. On physicochemical evaluation it was found to contain more amount of polar constituents as the ethanol extractive value was found to be more. Total ash value and acid insoluble ash indicated the presence of inorganic acids and silicaceous matter respectively. Foaming index and swelling index were indicative of saponins and mucilaginous matter present in the aerial parts. On preliminary phytochemical screening and thin layer chromatographic studies it revealed the presence of saponins, flavonoids, phenolic compounds and mucilage. Conclusion: The present work carried out can serve as a purpose for identification, authentication and standardization of the crude drug.

scholarly journals High-Performance Thin-Layer Chromatography for Rutin, Chlorogenic Acid, Caffeic Acid, Ursolic Acid, and Stigmasterol Analysis in Periploca aphylla Extracts

Separations ◽  
2021 ◽  
Vol 8 (4) ◽  
pp. 44
Author(s):  
Raha Orfali ◽  
Shagufta Perveen ◽  
Hanan Y. Aati ◽  
Perwez Alam ◽  
Omar M. Noman ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thin Layer ◽  
Chlorogenic Acid ◽  
Caffeic Acid ◽  
Ursolic Acid ◽  
Mobile Phase ◽  
High Performance ◽  
Folk Medicine ◽  
Aerial Parts ◽  
Layer Chromatography

Periploca aphylla (PA), an interesting Saudi medicinal plant, is used in folk medicine to treat urticaria, cerebral fever, tumors, and swelling. To prove its use in folk medicine, two different extracts from the aerial parts of the plant: chloroform P-1, and n-butanol P-2 were subjected to biological assays to screen peroxisome proliferator-activated receptors (PPARα and PPARγ) agnostic, anti-inflammatory, antioxidant, cytotoxic, and estrogenic activities. In addition, five bioactive secondary metabolites were isolated from the aerial parts of the plant: rutin, chlorogenic acid, caffeic acid, ursolic acid, and stigmasterol. P-1 and P-2 decreased cellular oxidative stress by 47.0% and 62.0%, respectively, compared to the standard drug quercetin, while one of the compounds rutin PA-1 isolated from P-1 extract and significantly decreased cellular oxidative stress by 67.0% compared to quercetin (75.0%). P-1 and P-2 also significantly activated PPARγ agnostic. P-1 and P-2 did not inhibit nuclear factor kappa B and inducible nitric oxide synthase activity and showed no cytotoxic or estergenic effects on four human cancer cell lines. In this study, both extracts were standardized using high-performance thin-layer chromatography (HPTLC). RP-HPTLC showed sharp and compact bands of rutin (Rf = 0.09), caffeic acid (Rf = 0.25), and chlorogenic acid (Rf = 0.39) scanned at λmax = 340 nm using the water: methanol (60:40 v/v) mobile phase. At λmax = 539 nm ursolic acid (Rf = 0.20) and stigmasterol (Rf = 0.48) were scanned using the chloroform: methanol (98:2 v/v) as NP-HPTLC mobile phase. Therefore, the developed RP- and NP-HPTLC systems are a precise, sensitive, and specific analytical tool for the quantification of compounds isolated from PA, which can be used as phytomarkers for taxonomical identification and assessment of PA.

scholarly journals Diurnal variation of the chemical composition and its repercussion on the biological activity polyphenolics of Salvia officinalis aerial parts

2019 ◽  
pp. 7-21
Author(s):  
Safa Rguez ◽  
amel Msaada ◽  
Majdi Hammami ◽  
Mejda Daami Remadi ◽  
Ikbel Chayeb ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Phenolic Compounds ◽  
Diurnal Variation ◽  
Biological Activities ◽  
Salvia Officinalis ◽  
Total Polyphenols ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Total Antioxidant

This study reported the diurnal variation of the chemical composition and biological activities of phenolic compounds of Salvia officinalis cultivated in Tunisia. The HPLC determination of different phenolic compounds showed that the amounts of total polyphenols reach its maximum at 7 am. Estimation of the antioxidant activity of different methanolic extracts of Salvia officinalis showed that the extract of 5 pm had the most effective antiradical power against DPPH and the best iron reducing capacity. The strongest total antioxidant capacity was recorded for extracts of 12 and 5 pm. The methanol extracts of sage also have a capacity to reduce the appetence of Spodoptera littoralis larvae. This activity did not vary with the harvesting time.

scholarly journals Chemical Profile of Essential Oils and Headspace Volatiles of Chaerophyllum hirsutum from Serbia

2017 ◽  
Vol 12 (9) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Goran M. Petrović ◽  
Jelena G. Stamenković ◽  
Gordana S. Stojanović ◽  
Violeta D. Mitić ◽  
Bojan K Zlatković
Keyword(s):  
Chemical Composition ◽  
Phenolic Compounds ◽  
Essential Oils ◽  
Chemical Profile ◽  
Headspace Volatiles ◽  
Dominant Class ◽  
Aerial Parts ◽  
Main Class ◽  
Main Component ◽  
Root Essential Oil

The present study reports the chemical composition on the headspace volatiles and essential oils obtained from fresh roots, shoots and inflorescences of Chaerophyllum hirsutum. In headspace samples, the most dominant class was monoterpene hydrocarbons, but the main compounds were different. The root had β-phellandrene as the major compound. Sabinene was the main component in the shoots volatiles, while the inflorescence was dominated by endo-fenchyl acetate. The composition of the essential oils of the aerial parts and the oil obtained from root was found to be quite different. The root essential oil showed the presence of phenolic compounds (13.2%), while in the oils from the aerial parts these compounds were present in very small amounts (0.1% in the shoots and 0.2% in the inflorescences oil). In total, 70 compounds were identified in the oil isolated from the root, with γ-terpinene (15.8%) as the major compound of the oil. In the oils isolated from the shoots and the inflorescences, for which 55 and 69 compounds were identified, respectively, the main class was represented by oxygenated sesquiterpenes with acorenone B as the main component, representing 57.0% of the shoots and 44.6% of the inflorescences.

scholarly journals Phenolic Compounds of Propolis from the Boreal Coniferous Zone

2012 ◽  
Vol 56 (1) ◽  
pp. 13-22 ◽  
Author(s):  
Anneli Salonen ◽  
Sanna Saarnio ◽  
Riitta Julkunen-Tiitto
Keyword(s):  
Phenolic Compounds ◽  
Caffeic Acid ◽  
Cinnamic Acid ◽  
Geographical Origin ◽  
Caffeic Acid Phenethyl Ester ◽  
Cinnamic Acid Derivatives ◽  
Caffeic Acid Derivatives ◽  
Central European ◽  
Phenolic Profiles ◽  
First Time

Phenolic Compounds of Propolis from the Boreal Coniferous ZoneThe profile of phenolic compounds in 19 propolis samples from different provinces in Finland were analysed for the first time using HPLC-DAD. Nine individual flavonoids (comprising 26% of the identified phenolics), eleven cinnamic acid derivatives (36%), two caffeic acid derivatives (14%), three chlorogenic acid derivatives (14%), and three other phenolic acids (10%) were found in the propolis samples. The compounds found in the largest quantities were methyl-naringenin and caffeic acid phenethyl ester (CAPE). The phenolic profiles of Finnish propolis show marked differences when compared withP. nigraandP. tremuloidespropolis of Central European and Canadian origins. The phenolic compounds found in propolis samples are commonly found in the tree species growing in Finland. Non-metric multidimensional scaling showed that samples were scattered and they did not form clear groups according to the geographical origin or age of the sample.

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