Determination of Polyphenols Content and Antioxidant Activity of Citrus sinensis Fruit Peel

2022 ◽  
Vol 11 (6) ◽  
pp. 716-724
Author(s):  
ABDELKADER BASLI ◽  
Ahmed Benmahammed
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Citrus Sinensis ◽  
Antioxidant Activities ◽  
Northern Region ◽  
The Body ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Fruit Peel ◽  
Therapeutic Benefits

Citrus fruits have long been qualified as veritable foods in view of the many therapeutic benefits they bring to the body. Several researchers have stud-ied the relationship between the bioactive compounds of Citrus and the health benefits and reduction of the risk of disease. Citrus sinensis, used in the food industry and its extracts have also been used in traditional medicine to activate vital energy, circulation, and weight loss, and appetite control. However, limited efforts have been made on collecting data on antioxidant potential of peels orange from the northern region of Algeria. Our study, therefore, focuses on the evaluation of total polyphenols compounds and in vitro assessment of their antioxidant potential of peels orange from the northern region of Algeria. The ethyl acetate and n-butanolic fractions from peels orange have been tested for their antioxidant activities and their lipid peroxidation inhibiting effects. The total phenolic and flavonoids content showed high levels. The preliminary phytochemical screening of tannins, phenolic compounds, flavonoids, coumarin, and alkaloids was also used. DPPH assay possesses strong potency to scavenge free radicals. The NO. radi-cal scavenging test exerts a good inhibitory effect. Furthermore, orange peels have been shown to suppress the lipid peroxidation of linoleic acid. Results further revealed a strong correlation between antioxidant effects and polyphenolic compounds. The high antioxidant activity of peel orange suggests that it could serve as a good natural antioxidant additive or food dietary supplement.

scholarly journals Antioxidant activities of aqueous extracts from nine different rose cultivars

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Hamadia Khurshid ◽  
Syed Mubashar Sabir ◽  
Shahid Iqbal Awan ◽  
Syed Rizwan Abbas ◽  
Muhammad Irshad
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Liver Homogenate ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Aqueous Extracts ◽  
Dpph Radical ◽  
Rose Petals

Rose petals have been applied as food additives in teas, cakes and flavor extracts. The aim of this research study was to explore and reveal the antioxidant potential of aqueous extracts of rose petals belonging to nine genotypes of rose (wild as well as hybrid). The in vitro antioxidant activities of roses were studied by lipid peroxidation assay, DPPH radical scavenging assay, iron chelation assay, phosphomolybdenum reduction assay and total phenolic and flavonoid contents. The aqueous extract showed inhibition against lipid peroxidation (TBARS), induced by prooxidants (10 µM FeSO4) in mice liver homogenate. The free radical scavenging activities of the extracts were determined by scavenging of the DPPH radical. Extracts also showed metal chelating activities and high antioxidant activity in the phosphomolybdenum assay. The high content of phenolics and flavonoids detected in aqueous extracts may be responsible for the antioxidant activity. Amongst the different rose genotypes, screened, Rosa moschata (musk rose) was found to carry slightly higher antioxidant potential, owing to its higher phytochemical content.

scholarly journals EKSTRAKSI DAN PENGAMATAN AKTIVITAS ANTIOKSIDAN DAN ANTIMIKROBA DARI KULIT BUAH POMETIA PINNATA

2017 ◽  
Vol 11 (2) ◽  
pp. 80 ◽  
Author(s):  
Fransisca C. Faustina ◽  
Filiana Santoso
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Crude Extract ◽  
Inhibitory Concentration ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Fruit Peel ◽  
Crude Extracts

<p align="justify">Studi ini meneliti ekstraksi kulit buah matoa (Pometia pinnata), buah dari famili Sapindaceae. Ekstraksi dilakukan dengan teknik maserasi menggunakan 3 pelarut: aseton, etanol dan akuades. Ekstrak tersebut kemudian dievaluasi aktivitas antioksidan dan antimikrobanya. Tes antimikroba menggunakan tes difusi lempeng dan difusi cairan. Aktivitas antioksidan tertinggi dihasilkan oleh ekstrak aseton dengan nilai IC50 15.323 ppm, kemudian ekstrak etanol 143.23 ppm dan 451.306 ppm. Aktivitas antioksidan dari ketiga ekstrak tersebut dibandingkan dengan aktivitas antioksidan dari asam askorbat dan ekstrak aseton menunjukkan kekuatan antioksidan setara dengan 50% dari kekuatan antioksidan dari asam askorbat. Aktivitas anti mikroba dievaluasi terhadap Escherichia coli, Bacillus cereus dan Staphylococcus aureus. Hasil evaluasi menunjukkan bahwa semua ekstrak memiliki aktivitas antimikroba dengan karakter bakteriostatis. Karena itu nilai NIC (Non-inhibitory Concentration) ditetapkan untuk semua ekstrak. Aktivitas tertinggi ditunjukkan oleh ekstrak aseton dan etanol dengan NIC &lt;0.5 ppm terhadapsemua bakteri tes, sementara aktivitas terlemah ditunjukkan oleh ekstrak akuades dengan NIC 5 ppm. Pengukuran kadar fenolik dari semua ekstrak dilakukan dengan tes Folin-Ciocalteau, dan ditemukan adanya korelasi positif antara kadar fenolik dan aktivitas antioksidan dari semua ekstrak. Analisa komponen dengan tes tannin, saponin dan spektrometri juga dilakukan dan hasil menunjukkan adanya kandungan tannin, saponin dan alkaloid di dalam kulit buah matoa.</p><p><strong>Kata kunci :</strong>Matoa, Pometia pinnata, Ekstraksi, Antimikroba, Antioksidan</p><hr /><p align="center"><strong>English Version Abstract</strong></p><p align="justify">This study investigated the extraction of matoa (Pometia pinnata) fruit peels, a fruit in the family of Sapindaceae. The extraction was performed through maceration method using three kinds of solvent: acetone, ethanol and water. The extracts were tested for their antioxidant and antimicrobial activities. DPPH radical scavenging assay was used in evaluating the antioxidant activities. The antimicrobial activity was evaluated using disc-diffusion and broth dilution method. The highest antioxidant activity was showed by acetone crude extract with IC50 value at 15.323 ppm, followed by ethanol and water crude extract at 143.23 ppm and 451.306 ppm, respectively. The antioxidant activities of the crude extracts were compared to L-ascorbic acid and it was found that acetone crude extract exhibited half the strength of the antioxidant activity of L-ascorbic acid. Antimicrobial activity of matoa fruit peel extracts were tested against Escherichia coli, Bacillus cereus and Staphylococcus aureus. The results showed that all crude extracts showed antimicrobial activity with bacteriostatic characteristic. Therefore, Non-Inhibitory Concentration (NIC) was determined for all crude extracts. Acetone and ethanol crude extract possessed the strongest antimicrobial activity at NIC &lt;0.5 ppm against all tested microorganisms, while water crude extract showed the weakest antimicrobial activity with NIC at 5 ppm. The total phenolic content of the crude extracts were measured using Folin-Ciocalteau method. Positive correlation between the antioxidant activities and the total phenolic content of the crude extracts was detected. Saponin and tannin test, as well as spectrometric analyses supported the presence of saponin, tannin and alkaloid in matoa fruit peel.</p><p><strong>Keywords :</strong>Matoa, Pometiapinnata, Extraction, Antimicrobial, Antioxidant</p>

scholarly journals Antibacterial and antioxidant activities of ethanol extract of Artocarpus lacucha Buch-Ham. leaves against Propionibacterium acne

2021 ◽  
Vol 332 ◽  
pp. 08007
Author(s):  
Panal Sitorus ◽  
Mahatir Muhammad ◽  
Henni Cintya ◽  
and Denni Satria
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
The Body ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Degenerative Diseases ◽  
Inhibitor Concentration ◽  
Propionibacterium Acne

Antioxidants can neutralize free radicals that can affect biological systems in the body that can cause degenerative diseases. Antibacterial active molecules have been identified from plants, and these chemicals are being utilized to develop new and promising treatments. The Mobe plant (Artocarpus lacucha Buch-Ham.) is a species of Artocarpus member of the Moraceae family. Includes a high proportion of phenolic compounds (flavonoids and phenolic acid). This research obtained to know about the biological activity of Artocarpus lacucha Buch-Ham and their total flavonoid and total phenolic content. The extract was prepared using the soxhletation technique using ethanol as the solvent. Antibacterial activity was determined using minimum inhibitor concentration. Propionibacterium acne can be inhibited at concentrations ranging from 300 ppm to 25 ppm. The DPPH technique was used to assess antioxidant activity. The samples’ total flavonoid and phenolic content were measured colorimetrically. The DPPH test revealed an antioxidant activity of 23.33 g/mL, which was used to compute the IC50. The extract’s total phenolic (117.44 mg GAE/g) and flavonoid (3.54 mg QE/g) content were determined to be high.

scholarly journals ANTIOXIDANT ACTIVITY OF ALKALOID FRACTIONS OF ZANTHOXYLUM ACANTHOPODIUM DC. FRUITS WITH 1,1-DIPHENYL-2-PICRYLHYDRAZYL ASSAY

2018 ◽  
Vol 11 (13) ◽  
pp. 33
Author(s):  
Rosidah Rosidah ◽  
Poppy Anjelisa Zaitun Hasibuan ◽  
Ginda Haro ◽  
Puteri Masri ◽  
Denny Satria
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Liquid Extraction ◽  
The Body ◽  
Antioxidant Potential ◽  
Liquid Liquid Extraction

 Objective: Antioxidants are vital substances which possess the ability to protect the body from damage caused by free radical-induced oxidative stress. The purpose of this study is to determine antioxidant activities of alkaloid fractions of Zanthoxylum acanthopodium DC. fruits.Methods: Z. acanthopodium DC. fruit was extracted by maceration. Ethanol extract was fractionated with liquid-liquid extraction using n-hexane and chloroform at pH 3, 7, 9, and 11 to obtained alkaloid fractions. Antioxidant activity for extract and fractions was determined with 1,1-diphenyl-2- picrylhydrazyl.Results: The IC50 of extract and fractions was 101.11±0.11, 273.24±0.12, 116.62±0.04, 61.12±0.01, 23.15±0.01, and 112.39±0.04 μg/mL, respectively.Conclusion: The results reveal that alkaloid fractions of Z. acanthopodium DC. fruit have very strong antioxidant potential. Our further study is to isolate the alkaloid compounds.

scholarly journals Protective effect of moringa-based beverages against hyperlipidemia and hyperglycemia in type 2 diabetes-induced rats

Food Research ◽  
2021 ◽  
Vol 5 (2) ◽  
Author(s):  
M.M. Al Zhrani ◽  
S.A. Althwab ◽  
T. Aljutaily ◽  
H.A. Alfheeaid ◽  
I.S. Ashoush ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Oral Administration ◽  
Protective Effect ◽  
Islets Of Langerhans ◽  
Antioxidant Activities ◽  
Biological Evaluation ◽  
Diabetic Rats ◽  
Total Phenolic ◽  
Total Carotenoids ◽  
Therapeutic Benefits

The objectives of this study were to determine the phenolic profile and potential antioxidant activity of Moringa oleifera leaves (MOL) cultivated in Saudi Arabia and to investigate the protective effect of moringa-based beverages on lipidemic and glycemic parameters in alloxan-induced diabetic rats (DR). The total phenolic content (TPC), chlorophylls, total carotenoids, total flavonoids, total flavonols, ascorbic acid content, and antioxidant activities (AOA) of MOL were determined and HPLC analysis of MOL was quantitatively performed. Moringa beverages as pure moringa (PM) and ginger-flavored moringa (GM) were given to diabetic rats (DR) at a dose of 1 mL 100 g-1 body weight/day for a successive six weeks. Glucose level (GL), lipid profile, and antioxidant parameters including malondialdehyde (MDA), reduced glutathione (GSH), and pancreas histopathological alterations were examined. Results indicated that MOL and prepared moringa-based beverages possess a suppressive amount of phenolics with considerable antioxidant activity in vitro. TPC was 78.72 mg GAE g-1 DW possess AOA as 36.33 μmol TE g-1 DW. Among the eleven identified phenolic acids and six flavonoids in MOL, caffeic acid and catechin were predominant and recorded 151.01 and 736.30 mg/100 g, respectively. Administration of PM or GM beverages significantly decreased GL and significantly improved serum lipid profiles in the DR group. Interestingly, oral administration of PM and GM beverages significantly attenuated the MDA and GSH levels in the DR compared to normal rats. Histopathologically, severe atrophy was noted in the islets of Langerhans in the DR group. However, after six weeks of oral administration of PM beverage, only mild atrophy was recorded in the islets of Langerhans, while with GM beverage, no histopathological alteration in the islets of Langerhans was found. The nutritional and biological evaluation of the moringa-based beverages revealed that both PM and GM beverages exhibit high antioxidant activity, possess antihyperglycemic and antihyperlipidemic effects, and alleviated alloxan-induced pancreatic damage in DR. Moringa could be recommended for diabetes and utilized for its great therapeutic benefits.

SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DAN ANTITUMOR 20 TUMBUHAN OBAT ASAL KABUPATEN MANOKWARI

2008 ◽  
Vol 7 (1) ◽  
Author(s):  
Bimo Budi Santoso
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Antitumor Activity ◽  
Traditional Method ◽  
Extraction Efficiency ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents

<strong><em>Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed.  The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents,  A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R<sup>2</sup>= 0,77) and methanol (R<sup>2</sup> = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher.</em></strong>

scholarly journals Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
E. S. Prasedya ◽  
A. Frediansyah ◽  
N. W. R. Martyasari ◽  
B. K. Ilhami ◽  
A. S. Abidin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Particle Sizes ◽  
Epicatechin Gallate ◽  
Particle Size Reduction ◽  
Phytochemical Composition

AbstractSample particle size is an important parameter in the solid–liquid extraction system of natural products for obtaining their bioactive compounds. This study evaluates the effect of sample particle size on the phytochemical composition and antioxidant activity of brown macroalgae Sargassum cristaefolium. The crude ethanol extract was extracted from dried powders of S.cristeafolium with various particle sizes (> 4000 µm, > 250 µm, > 125 µm, > 45 µm, and < 45 µm). The ethanolic extracts of S.cristaefolium were analysed for Total Phenolic Content (TPC), Total Flavonoid Content (TFC), phenolic compound concentration and antioxidant activities. The extract yield and phytochemical composition were more abundant in smaller particle sizes. Furthermore, the TPC (14.19 ± 2.08 mg GAE/g extract to 43.27 ± 2.56 mg GAE/g extract) and TFC (9.6 ± 1.8 mg QE/g extract to 70.27 ± 3.59 mg QE/g extract) values also significantly increased as particle sizes decreased. In addition, phenolic compounds epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and Epigallocatechin gallate (EGCG) concentration were frequently increased in samples of smaller particle sizes based on two-way ANOVA and Tukey’s multiple comparison analysis. These results correlate with the significantly stronger antioxidant activity in samples with smaller particle sizes. The smallest particle size (< 45 µm) demonstrated the strongest antioxidant activity based on DPPH, ABTS, hydroxyl assay and FRAP. In addition, ramp function graph evaluates the desired particle size for maximum phytochemical composition and antioxidant activity is 44 µm. In conclusion, current results show the importance of particle size reduction of macroalgae samples to increase the effectivity of its biological activity.

scholarly journals Bioactive Compounds, Antioxidant Activity and Growth Behavior in Lettuce Cultivars Grown under Field and Greenhouse Conditions

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 52
Author(s):  
Melisa Lanza Volpe ◽  
Verónica C. Soto Vargas ◽  
Anabel Morón ◽  
Roxana E. González
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Growth Conditions ◽  
Growth Behavior ◽  
Total Phenolic ◽  
Spectrophotometric Methods ◽  
Leafy Greens ◽  
Growing Conditions ◽  
Field Production

Lettuce (Lactuca sativa L.) is one of the most important leafy greens worldwide. The nutritional value of its edible leaf depends on different factors including type and growing conditions. The aim was to determine the bioactive compounds content, antioxidant activity and growth behavior of twenty-two lettuce genotypes, cultivated under field and greenhouse conditions. Total phenolic compound, chlorophylls, carotenoids, anthocyanin contents and antioxidant activities were analyzed by spectrophotometric methods. Data were analyzed by analysis of variance (ANOVA). Significant differences between bioactive compounds, antioxidant activity and growth behavior were found among cultivars and morphological types, for both growth conditions. Carotenoid and chlorophyll content was higher in greenhouse conditions for all genotypes. In field production, butterhead and iceberg lettuces showed lower content of these bioactive compounds. The red-pigmented Falbala cultivar from field production showed the highest level of polyphenols and anthocyanin. Meanwhile, in greenhouse conditions, the oak leaf cultivar Grenadine displayed the highest concentration of these phenolic compounds. The iceberg type lettuce showed the lowest percentages of antioxidant activity in both environments. The results showed the effect of growing conditions and the high variability in lettuce bioactive compounds content and antioxidant activity among the different types.

scholarly journals HPLC-DAD Polyphenolic Profiling and Antioxidant Activities of Sorghum bicolor during Germination

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 417
Author(s):  
Ioana Otilia Ghinea ◽  
Maria Daniela Ionica Mihaila ◽  
Giorgiana-Valentina Blaga (Costea) ◽  
Sorin Marius Avramescu ◽  
Mihaela Cudalbeanu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Sorghum Bicolor ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Ultrasound Assisted Extraction ◽  
Food Ingredient ◽  
Radical Scavenger Activity ◽  
Scavenger Activity ◽  
Sorghum Grains

The purpose of this study was to assess the suitability of the Romanian Albanus hybrid of Sorghum bicolor as a potential functional food ingredient. Ultrasound-assisted extraction in different solvents, together with spectrophotometric and chromatographic methods, was used to monitor the variation in total phenolic and flavonoid content and the antioxidant activity of raw sorghum grains before and during short germination periods (24, 36 and 48 h). The High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) investigation of the extracts revealed that the ungerminated sorghum grains extracted with methanol had the highest diversity of phenolic compounds, while the total phenolic content (TPC) was the highest after 36 h of germination in both extract types: 1853 mg GAE/100 g for the methanolic extract and 1726 mg GAE/100 g for the ethanolic extract. The findings of this study showed that the TPC of sorghum extracts is strongly correlated with their antioxidant activity and, overall, that the studied extracts presented a good radical scavenger activity, which supports the benefits of alimentary uses of Sorghum bicolor grains.

scholarly journals Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
K. Nagendra Prasad ◽  
Jing Hao ◽  
Chun Yi ◽  
Dandan Zhang ◽  
Shengxiang Qiu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Liver Carcinoma ◽  
Scavenging Activity ◽  
Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

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