Protective effect of moringa-based beverages against hyperlipidemia and hyperglycemia in type 2 diabetes-induced rats

The objectives of this study were to determine the phenolic profile and potential antioxidant activity of Moringa oleifera leaves (MOL) cultivated in Saudi Arabia and to investigate the protective effect of moringa-based beverages on lipidemic and glycemic parameters in alloxan-induced diabetic rats (DR). The total phenolic content (TPC), chlorophylls, total carotenoids, total flavonoids, total flavonols, ascorbic acid content, and antioxidant activities (AOA) of MOL were determined and HPLC analysis of MOL was quantitatively performed. Moringa beverages as pure moringa (PM) and ginger-flavored moringa (GM) were given to diabetic rats (DR) at a dose of 1 mL 100 g-1 body weight/day for a successive six weeks. Glucose level (GL), lipid profile, and antioxidant parameters including malondialdehyde (MDA), reduced glutathione (GSH), and pancreas histopathological alterations were examined. Results indicated that MOL and prepared moringa-based beverages possess a suppressive amount of phenolics with considerable antioxidant activity in vitro. TPC was 78.72 mg GAE g-1 DW possess AOA as 36.33 μmol TE g-1 DW. Among the eleven identified phenolic acids and six flavonoids in MOL, caffeic acid and catechin were predominant and recorded 151.01 and 736.30 mg/100 g, respectively. Administration of PM or GM beverages significantly decreased GL and significantly improved serum lipid profiles in the DR group. Interestingly, oral administration of PM and GM beverages significantly attenuated the MDA and GSH levels in the DR compared to normal rats. Histopathologically, severe atrophy was noted in the islets of Langerhans in the DR group. However, after six weeks of oral administration of PM beverage, only mild atrophy was recorded in the islets of Langerhans, while with GM beverage, no histopathological alteration in the islets of Langerhans was found. The nutritional and biological evaluation of the moringa-based beverages revealed that both PM and GM beverages exhibit high antioxidant activity, possess antihyperglycemic and antihyperlipidemic effects, and alleviated alloxan-induced pancreatic damage in DR. Moringa could be recommended for diabetes and utilized for its great therapeutic benefits.

Download Full-text

Determination of Polyphenols Content and Antioxidant Activity of Citrus sinensis Fruit Peel

South Asian Journal of Experimental Biology ◽

10.38150/sajeb.11(6).p716-724 ◽

2022 ◽

Vol 11 (6) ◽

pp. 716-724

Author(s):

ABDELKADER BASLI ◽

Ahmed Benmahammed

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activity ◽

Citrus Sinensis ◽

Antioxidant Activities ◽

Northern Region ◽

The Body ◽

Antioxidant Potential ◽

Total Phenolic ◽

Fruit Peel ◽

Therapeutic Benefits

Citrus fruits have long been qualified as veritable foods in view of the many therapeutic benefits they bring to the body. Several researchers have stud-ied the relationship between the bioactive compounds of Citrus and the health benefits and reduction of the risk of disease. Citrus sinensis, used in the food industry and its extracts have also been used in traditional medicine to activate vital energy, circulation, and weight loss, and appetite control. However, limited efforts have been made on collecting data on antioxidant potential of peels orange from the northern region of Algeria. Our study, therefore, focuses on the evaluation of total polyphenols compounds and in vitro assessment of their antioxidant potential of peels orange from the northern region of Algeria. The ethyl acetate and n-butanolic fractions from peels orange have been tested for their antioxidant activities and their lipid peroxidation inhibiting effects. The total phenolic and flavonoids content showed high levels. The preliminary phytochemical screening of tannins, phenolic compounds, flavonoids, coumarin, and alkaloids was also used. DPPH assay possesses strong potency to scavenge free radicals. The NO. radi-cal scavenging test exerts a good inhibitory effect. Furthermore, orange peels have been shown to suppress the lipid peroxidation of linoleic acid. Results further revealed a strong correlation between antioxidant effects and polyphenolic compounds. The high antioxidant activity of peel orange suggests that it could serve as a good natural antioxidant additive or food dietary supplement.

Download Full-text

Essential Oil Composition, Bioactive Compounds, and Antioxidant Activities in Iberis amara L.

Natural Product Communications ◽

10.1177/1934578x19846355 ◽

2019 ◽

Vol 14 (5) ◽

pp. 1934578X1984635 ◽

Cited By ~ 1

Author(s):

Pooran Golkar ◽

Fariborz Moattar

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Essential Oil Composition ◽

High Ratio ◽

Total Phenolic ◽

Oil Composition ◽

Leaf Extracts ◽

Total Carotenoids ◽

Iberis Amara ◽

Main Component

This research was carried out to assess essential oils (EOs), total phenolic content (TPC), total flavonoids (TFD), total flavonols (TFL), total chlorophyll, total carotenoids, total anthocyanins, and different antioxidant activity assays in two plant samples (leaf and bud) of Iberis amara L. The gas chromatography mass spectrophotometry (GC-MS) of the EOs and the head space (HS)-GC-MS of the methanolic leaf extracts identified 34 and 6 different compounds, respectively. The major components of the leaf EOs were carvacrol (9.4%), camphene (6.2%), р-cymene (4.3%), and eugenol (3.8%) respectively, whereas cumin aldehyde (10.4%) was the main component in the bud sample. 1-Butene, 4-isothiocyanate (50%) was identified as the main component in the HS-GC-MS analysis of leaves extract. The highest content of TPC (32.8 ± 0.7 mg GAE/gDW), TFD (28.4 ± 0.7 mg QE/gDW), TFL (11.8 ± 0.06 mg QE/gFW), and anthocyanin (0.4 ±0.02 µmol/g FW) was found in the methanolic extract of leaves. The highest antioxidant activity in the phosphomolibdate assay (628.3 ± 10 µg AAE/gFW) and the least activity in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay (IC50 = 415 ± 3.1 µg/mL) was found in leaf samples. The high ratio of monoterpene hydrocarbons and oxygenated monoterpenes of the EOs along with the high antioxidant activity propose the application of this medicinal plant for general or specific applications in food industries as a herbal plant.

Download Full-text

SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DAN ANTITUMOR 20 TUMBUHAN OBAT ASAL KABUPATEN MANOKWARI

Jurnal Natural ◽

10.30862/jn.v7i1.678 ◽

2008 ◽

Vol 7 (1) ◽

Author(s):

Bimo Budi Santoso

Keyword(s):

Antioxidant Activity ◽

Medicinal Plants ◽

Antitumor Activity ◽

Traditional Method ◽

Extraction Efficiency ◽

Phenolic Content ◽

Antioxidant Activities ◽

Total Phenolic ◽

Phenolic Contents ◽

Total Phenolic Contents

Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed. The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents, A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R2= 0,77) and methanol (R2 = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher.

Download Full-text

Antidyslipidemic and Antioxidant Activities of Matricaria pubescens (Desf.) Shultz.in Streptozotocin-Induced Diabetic Rats

Cardiovascular & Hematological Agents in Medicinal Chemistry ◽

10.2174/1871525718666200506100139 ◽

2020 ◽

Vol 18 ◽

Cited By ~ 1

Author(s):

Ayoub Amssayef ◽

Bouchra Azzaou ◽

Mohammed Ajebli ◽

Mohamed Eddouks

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Serum Levels ◽

Diabetic Rats ◽

Favorable Effect ◽

Lipoprotein Profile ◽

Phytochemical Compounds ◽

Asteraceae Family

Aims: The study aimed to evaluate the antihyperlipidemic and antioxidant activities of Matricaria pubescens. Background: Matricaria pubescens (Desf.) Shultz belongs to Asteraceae family and it is commonly used traditionally for handling diabetes mellitus. Objective: The objective of this study was to assess the antioxidant activity of Matricaria pubescens (Desf.) Shultz and its effect on lipid and lipoprotein profile in normal and streptozotocin-induced diabetic rats. Methods: The effect of repeated (7 days of treatment) oral administration of the aqueous extract of aerial part of Matricaria pubescens (MPAE) at a dose of 40 mg/kg on lipid and lipoprotein profile was examined in normal and streptozotocin-induced diabetic rats. Furthermore, a preliminary phytochemical screening and the quantification of phenolic, flavonoid and tannin contents as well as the antioxidant activity using two methods (FRAP and ABTS) were carried out. Results: MPAE demonstrated a potent antidyslipidemic effect in diabetic rats by reducing serum levels of triglycerides, total cholesterol and low-density lipoprotein (LDL). In addition, the results showed that the extract is rich in several phytochemical compounds and revealed an important antioxidant activity. Conclusion: In summary, this study proved that Matricaria pubescens (Desf.) Shultz. has a favorable effect on diabetic dyslipidemia.

Download Full-text

Antihyperglycemic, Antihyperlipidemic and Antioxidant Effects of Cotula cinerea (Del) in Normal and Streptozotocin-Induced Diabetic Rats

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530320666200513081312 ◽

2020 ◽

Vol 20 (9) ◽

pp. 1504-1513 ◽

Cited By ~ 1

Author(s):

Ayoub Amssayef ◽

Mohamed Eddouks

Keyword(s):

Antioxidant Activity ◽

Glucose Tolerance ◽

Oral Administration ◽

Beneficial Effect ◽

Aqueous Extract ◽

Aerial Part ◽

Diabetic Rats ◽

Histopathological Analysis ◽

Phytochemical Screening ◽

Cotula Cinerea

Aims: The current investigation aimed to assess the antioxidant, antidiabetic and antilipidemic effects of the aqueous extract of aerial part of Cotula cinerea (C. cinerea). Background: Cotula cinerea (Del). which belongs to the Asteraceae family is commonly used traditionally for the treatment of diabetes. Objective: The objective of the study was to study the effect of the aqueous C. cinerea extract on glucose and lipid metabolism in normal and streptozotocin-induced diabetic rats using a single and repeated oral administration. Methods: A preliminary phytochemical screening and the quantification of phenolic and flavonoid contents as well as the antioxidant activity using three methods (DPPH, FRAP and ABTS) were carried out. The effect of a single and repeated (15 days of treatment) oral administration of the aqueous extract of aerial part of Cotula cinerea (AEAPCC) at a dose of 20 mg/kg on glucose and lipid profile was examined in normal and streptozotocin-induced diabetic rats. Additionally, histopathological examination of the pancreas and liver was carried out according to the Hematoxylin-Eosin method. Results: AEAPCC (20 mg/kg) showed a significant blood glucose-lowering activity in both normal and diabetic rats after a single and repeated oral administration during 15 days. The aqueous extract was also able to decrease the plasma triglycerides levels in both normal and diabetic rats after 15 days of oral treatment at a dose of 20 mg/Kg while no effect was observed on plasma cholesterol levels. In addition, the results show that AEAPCC exhibits an in vitro antioxidant activity using different tests. Histopathological analysis of the pancreas and liver of AEAPCC-treated diabetic rats has revealed that AEAPCC had a beneficial effect on the architecture of these organs while no improvement of glucose tolerance was noticed using the glucose tolerance test. Furthermore, the results showed that the extract is rich in several phytochemical compounds and exhibited an important antioxidant activity. The phytochemical screening revealed that AEAPCC contains polyphenolic compounds, flavonoids, tannins, alkaloids, saponins, quinones, sterols, terpenoids, anthroquinones and reducing sugars. Whereas, it is free from glycosides. Conclusion: In conclusion, this study demonstrates that Cotula cinerea possesses a beneficial effect on diabetes. Further investigations are required to study the mechanism of action of the antidiabetic effect of this plant.

Download Full-text

Study of Antihyperglycemic, Antihyperlipidemic and Antioxidant Activities of Tannins Extracted from Warionia saharae Benth. & Coss

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530318666181029160539 ◽

2019 ◽

Vol 19 (2) ◽

pp. 189-198 ◽

Cited By ~ 3

Author(s):

Mohammed Ajebli ◽

Fadwa El Ouady ◽

Mohamed Eddouks

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Histopathological Examination ◽

Density Lipoprotein ◽

Hdl Cholesterol ◽

Diabetic Rats ◽

Lipid Lowering ◽

Glucose Levels ◽

Soxhlet Apparatus

Background and Objective: Warionia saharae Benth & Coss, a plant belonging to Asteraceae family, is used for its anti-diabetic properties in Morocco. The objective of this study was to evaluate the effect of tannins extracted from Warionia saharae (W. saharae) on blood glucose levels and lipid profile in normal and streptozotocin(STZ)-induced diabetic rats. Methods: Tannins (TE) were extracted from W. saharae using Soxhlet apparatus and different organic solvents. Single and once daily repeated oral administration of TE (10 mg/kg) for 15 days were used to evaluate the glucose and lipid-lowering activity in normal and diabetic rats. Furthermore, glucose test tolerance, liver histopathological examination and in vitro antioxidant activity of TE were carried out in this study. Results: The results showed that TE was able to exert antihyperglycemic and lowering total cholesterol effects as well as improvement of the high-density lipoprotein (HDL)-cholesterol serum level after 15 days of treatment. Furthermore, TE improved glucose tolerance, histopathological status of liver in diabetic rats and demonstrated interesting antioxidant activity. Conclusion: In conclusion, the present investigation revealed that TE possesses potent antidiabetic and antihyperlipidemic activities as claimed in different ethnopharmacological practices.

Download Full-text

Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

Scientific Reports ◽

10.1038/s41598-021-95769-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

E. S. Prasedya ◽

A. Frediansyah ◽

N. W. R. Martyasari ◽

B. K. Ilhami ◽

A. S. Abidin ◽

...

Keyword(s):

Antioxidant Activity ◽

Particle Size ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Ethanol Extract ◽

Total Phenolic ◽

Particle Sizes ◽

Epicatechin Gallate ◽

Particle Size Reduction ◽

Phytochemical Composition

AbstractSample particle size is an important parameter in the solid–liquid extraction system of natural products for obtaining their bioactive compounds. This study evaluates the effect of sample particle size on the phytochemical composition and antioxidant activity of brown macroalgae Sargassum cristaefolium. The crude ethanol extract was extracted from dried powders of S.cristeafolium with various particle sizes (> 4000 µm, > 250 µm, > 125 µm, > 45 µm, and < 45 µm). The ethanolic extracts of S.cristaefolium were analysed for Total Phenolic Content (TPC), Total Flavonoid Content (TFC), phenolic compound concentration and antioxidant activities. The extract yield and phytochemical composition were more abundant in smaller particle sizes. Furthermore, the TPC (14.19 ± 2.08 mg GAE/g extract to 43.27 ± 2.56 mg GAE/g extract) and TFC (9.6 ± 1.8 mg QE/g extract to 70.27 ± 3.59 mg QE/g extract) values also significantly increased as particle sizes decreased. In addition, phenolic compounds epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and Epigallocatechin gallate (EGCG) concentration were frequently increased in samples of smaller particle sizes based on two-way ANOVA and Tukey’s multiple comparison analysis. These results correlate with the significantly stronger antioxidant activity in samples with smaller particle sizes. The smallest particle size (< 45 µm) demonstrated the strongest antioxidant activity based on DPPH, ABTS, hydroxyl assay and FRAP. In addition, ramp function graph evaluates the desired particle size for maximum phytochemical composition and antioxidant activity is 44 µm. In conclusion, current results show the importance of particle size reduction of macroalgae samples to increase the effectivity of its biological activity.

Download Full-text

Bioactive Compounds, Antioxidant Activity and Growth Behavior in Lettuce Cultivars Grown under Field and Greenhouse Conditions

Proceedings ◽

10.3390/foods_2020-07709 ◽

2020 ◽

Vol 70 (1) ◽

pp. 52

Author(s):

Melisa Lanza Volpe ◽

Verónica C. Soto Vargas ◽

Anabel Morón ◽

Roxana E. González

Keyword(s):

Antioxidant Activity ◽

Bioactive Compounds ◽

Antioxidant Activities ◽

Growth Conditions ◽

Growth Behavior ◽

Total Phenolic ◽

Spectrophotometric Methods ◽

Leafy Greens ◽

Growing Conditions ◽

Field Production

Lettuce (Lactuca sativa L.) is one of the most important leafy greens worldwide. The nutritional value of its edible leaf depends on different factors including type and growing conditions. The aim was to determine the bioactive compounds content, antioxidant activity and growth behavior of twenty-two lettuce genotypes, cultivated under field and greenhouse conditions. Total phenolic compound, chlorophylls, carotenoids, anthocyanin contents and antioxidant activities were analyzed by spectrophotometric methods. Data were analyzed by analysis of variance (ANOVA). Significant differences between bioactive compounds, antioxidant activity and growth behavior were found among cultivars and morphological types, for both growth conditions. Carotenoid and chlorophyll content was higher in greenhouse conditions for all genotypes. In field production, butterhead and iceberg lettuces showed lower content of these bioactive compounds. The red-pigmented Falbala cultivar from field production showed the highest level of polyphenols and anthocyanin. Meanwhile, in greenhouse conditions, the oak leaf cultivar Grenadine displayed the highest concentration of these phenolic compounds. The iceberg type lettuce showed the lowest percentages of antioxidant activity in both environments. The results showed the effect of growing conditions and the high variability in lettuce bioactive compounds content and antioxidant activity among the different types.

Download Full-text

HPLC-DAD Polyphenolic Profiling and Antioxidant Activities of Sorghum bicolor during Germination

Agronomy ◽

10.3390/agronomy11030417 ◽

2021 ◽

Vol 11 (3) ◽

pp. 417

Author(s):

Ioana Otilia Ghinea ◽

Maria Daniela Ionica Mihaila ◽

Giorgiana-Valentina Blaga (Costea) ◽

Sorin Marius Avramescu ◽

Mihaela Cudalbeanu ◽

...

Keyword(s):

Antioxidant Activity ◽

Sorghum Bicolor ◽

Antioxidant Activities ◽

Total Phenolic ◽

Radical Scavenger ◽

Ultrasound Assisted Extraction ◽

Food Ingredient ◽

Radical Scavenger Activity ◽

Scavenger Activity ◽

Sorghum Grains

The purpose of this study was to assess the suitability of the Romanian Albanus hybrid of Sorghum bicolor as a potential functional food ingredient. Ultrasound-assisted extraction in different solvents, together with spectrophotometric and chromatographic methods, was used to monitor the variation in total phenolic and flavonoid content and the antioxidant activity of raw sorghum grains before and during short germination periods (24, 36 and 48 h). The High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) investigation of the extracts revealed that the ungerminated sorghum grains extracted with methanol had the highest diversity of phenolic compounds, while the total phenolic content (TPC) was the highest after 36 h of germination in both extract types: 1853 mg GAE/100 g for the methanolic extract and 1726 mg GAE/100 g for the ethanolic extract. The findings of this study showed that the TPC of sorghum extracts is strongly correlated with their antioxidant activity and, overall, that the studied extracts presented a good radical scavenger activity, which supports the benefits of alimentary uses of Sorghum bicolor grains.

Download Full-text

Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

Journal of Biomedicine and Biotechnology ◽

10.1155/2009/612805 ◽

2009 ◽

Vol 2009 ◽

pp. 1-6 ◽

Cited By ~ 29

Author(s):

K. Nagendra Prasad ◽

Jing Hao ◽

Chun Yi ◽

Dandan Zhang ◽

Shengxiang Qiu ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Liver Carcinoma ◽

Scavenging Activity ◽

Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

Download Full-text