scholarly journals In vitro activity of hybrid lavender essential oils against multidrug resistant strains of Pseudomonas aeruginosa

2018 ◽  
Vol 12 (01) ◽  
pp. 009-014 ◽  
Author(s):  
Matthew Donadu ◽  
Donatella Usai ◽  
Antonio Pinna ◽  
Tiziana Porcu ◽  
Vittorio Mazzarello ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Cell Model ◽  
Antimicrobial Properties ◽  
The United States ◽  
Multidrug Resistant ◽  
New Antibiotics ◽  
Mediterranean Regions ◽  
A Cell

Introduction: Lavender is an evergreen shrub native to Northern Africa and other mountainous Mediterranean regions. It grows throughout Southern Europe, the United States, and Australia. Lavender essential oil has been used since ancient times and is known for its anti-inflammatory, antidepressant, antiseptic, antifungal and antimicrobial properties. Methodology: in this study, the antimicrobial activity of two Lavender essential oils (Lavanda sumian and Lavanda grosso) against 16 multidrug-resistant P. aeruginosa strains from clinical ocular samples taken from migrant patients has been investigated. The in vitro cytotoxic activity on human Wong-Kilbourne derivative (WKD) conjunctiva cells from healthy patients and nitric oxide synthase (NOS) activity on murine macrophage (J774.1A) were also evaluated. Results: L. sumian showed lower antimicrobial activity when compared to L. grosso. Both lavender oils tested had no cytotoxic effect at very low concentrations, mostly L. grosso. The essential oils extracted from L. sumian and L. grosso significantly reduced NOS in a cell model. Conclusion: Increase in drug resistance and lack of new antibiotics may encourage the development of natural antimicrobial treatments.

scholarly journals In vitro activity of essential oils against Pseudomonas aeruginosa isolated from infected hip implants

2018 ◽  
Vol 12 (11) ◽  
pp. 996-1001 ◽  
Author(s):  
Veronica Amorese ◽  
Matthew Donadu ◽  
Donatella Usai ◽  
Angelino Sanna ◽  
Fabio Milia ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Antimicrobial Properties ◽  
Multidrug Resistant ◽  
Thymus Vulgaris ◽  
Hip Implants ◽  
Melaleuca Alternifolia ◽  
New Antibiotics ◽  
Normal Human

Introduction: Essential oils have been used since ancient times and are known for their anti-inflammatory, anti-depressive, antiseptic, antifungal and antimicrobial properties. Methodology: in this study the antimicrobial activity of two essential oils from Melaleuca alternifolia and Thymus vulgaris–red thyme geraniol was tested against 16 multidrug-resistant P. aeruginosa strains from infected hip implants as well as the “in vitro” cytotoxic activity on normal human Wong-Kilbourne derivative (WKD) cells. Results: Thymus vulgaris–red thyme geraniol showed lower antimicrobial activity when compared to Melaleuca alternifolia. All tested oils were cytotoxic at concentrations lower than 0.12%. Conclusion: Increase in drug resistance and lack of new antibiotics may encourage the development of natural treatments together with higher concern on environmental issues and natural lifestyle.

Antimicrobial Activity of Differently Concentrated Nanoparticle Dispersions

2017 ◽  
Vol 13 ◽  
pp. 239-247 ◽  
Author(s):  
Georgy A. Frolov ◽  
Yakov N. Karasenkov ◽  
Alexander A. Gusev ◽  
Olga V. Zakharova ◽  
Anna Yu. Godymchuk ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Dental Plaque ◽  
Dispersion Medium ◽  
Antimicrobial Properties ◽  
Dispersed Phase ◽  
Colloidal Solutions ◽  
Nanoparticle Dispersions ◽  
New Antibiotics

Preparation of nontoxic antimicrobial solutions is a priority in the development of new antibiotics. The in vitro effect of the chemical composition of the dispersed phase and dissolved solids in the dispersion medium on the antimicrobial properties of aqueous colloidal solutions of nanoparticles of a number of metals and metal oxides against microorganisms of different species - bacterial dental plaque, Bacillus cereus, and spores of vaccine strain Bacillus anthracis STI - 1 were studied in this work.

scholarly journals In Vitro Susceptibility of Multidrug Resistant Strains of Salmonella to Essential Oils

2020 ◽  
Vol 15 (1) ◽  
pp. 1934578X1987890
Author(s):  
Valeria Listorti ◽  
Roberta Battistini ◽  
Carlo Ercolini ◽  
Clara Tramuta ◽  
Elisabetta Razzuoli ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Resistance ◽  
Essential Oils ◽  
Multidrug Resistant ◽  
Animal Origin ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
In Vitro Susceptibility ◽  
Resistant Strains

Antimicrobial resistance has become a global threat to public health. There is a critical need to find new antimicrobial substances from natural sources. The aim of this study was to investigate the antimicrobial activity of essential oils (EOs) obtained from Origanum vulgare, Thymus serpyllum, Thymus vulgaris, and Melaleuca alternifolia against multidrug resistant strains of Salmonella isolated from samples of diverse animal origin. The strains were biochemically identified, serotyped, and characterized for their antimicrobial resistance profiles. The antimicrobial activity of the EOs against the strains was evaluated using the Kirby-Bauer diffusion method, followed by determination of the minimal inhibitory concentration and minimum bactericidal concentrations. The EOs of T. serpyllum and O. vulgare, which contain carvacrol as the main compound, show excellent antimicrobial activity.

scholarly journals Antimicrobial Activity of Some Essential Oils against Methicillin-Susceptible and Methicillin-Resistant Staphylococcus pseudintermedius-Associated Pyoderma in Dogs

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1782 ◽  
Author(s):  
Francesca Paola Nocera ◽  
Simone Mancini ◽  
Basma Najar ◽  
Fabrizio Bertelloni ◽  
Luisa Pistelli ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Multidrug Resistant ◽  
Therapeutic Approaches ◽  
Cinnamomum Zeylanicum ◽  
Staphylococcus Pseudintermedius ◽  
Methicillin Resistant ◽  
Resistant Strains ◽  
The One

This study aimed to test in vitro the antimicrobial activity of 11 essential oils (EOs) against four methicillin-resistant Staphylococcus pseudintermedius (MRSP) and four methicillin-susceptible S. pseudintermedius (MSSP) clinical isolates. The obtained findings demonstrated a clear in vitro efficacy of some tested EOs against both MRSP and MSSP strains. Particularly, modal minimum inhibitory concentration (MIC) values ranging from 1:2048 v/v for Melissa officinalis against an MSSP strain to 1:256 v/v for Cymbopogoncitratus against all MRSP strains were observed. The best results, highlighting a modal MIC value of 1:1024 v/v for all tested isolates, was provided by Cinnamomum zeylanicum. Intriguingly, Cinnamomum zeylanicum showed, in many cases, a correspondence between minimum bactericidal concentration (MBC) and MIC values, indicating that the inhibiting dose is also often bactericidal. Moreover, a mild antibacterial and bactericidal activity against both MRSP and MSSP isolates was detected for the other tested EOs. Considering the zoonotic potential of S. pseudintermedius and the increased dissemination of multidrug-resistant strains, the employment of EOs could be useful for the treatment of canine pyoderma. Since antibiotic resistance has become the most urgent issue, from the perspective of the One Health initiative, alternative therapeutic approaches are desirable to limit the use of antibiotics or to improve the efficacy of conventional therapies.

scholarly journals In Vitro Evaluation of Antimicrobial Activity and Cytotoxicity of Different Nanobiotics Targeting Multidrug Resistant and Biofilm Forming Staphylococci

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Mennatallah A. Mohamed ◽  
Maha Nasr ◽  
Walid F. Elkhatib ◽  
Wafaa N. Eltayeb
Keyword(s):  
Antimicrobial Activity ◽  
Rat Hepatocytes ◽  
Antibacterial Activities ◽  
Multidrug Resistant ◽  
Antimicrobial Activities ◽  
Antibiotic Resistant ◽  
Diphenyltetrazolium Bromide ◽  
New Antibiotics ◽  
Conventional Antibiotics

Antibiotic-resistant and biofilm-forming bacteria have surprisingly increased over recent years. On the contrary, the rate of development of new antibiotics to treat these emerging superbugs is very slow. Therefore, the aim of this study was to prepare novel nanobiotic formulations to improve the antimicrobial activity of three antibiotics (linezolid, doxycycline, and clindamycin) against Staphylococci. Antibiotics were formulated as nanoemulsions and evaluated for their antimicrobial activities and cytotoxicities. Cytotoxicity of the conventional antibiotics and nanobiotics was analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on rat hepatocytes. Half-maximal inhibitory concentration (IC50) was estimated from an experimentally derived dose-response curve for each concentration using GraphPad Prism software. Upon quantitative assessment of Staphylococcus biofilm formation, eighty-four isolates (66.14 %) were biofilm forming. Linezolid and doxycycline nanobiotics exhibited promising antibacterial activities. On the contrary, clindamycin nanobiotic exhibited poor antibacterial activity. Minimum biofilm inhibitory concentrations showed that 73.68 %, 45.6%, and 5.2% of isolates were sensitive to linezolid, doxycycline, and clindamycin nanobiotics, respectively. Results of this study revealed that antibiotics loaded in nanosystems had a higher antimicrobial activity and lower cytotoxicities as compared to those of conventional free antibiotics, indicating their potential therapeutic values.

scholarly journals Antimicrobial Activity of Selected Essential Oils against Selected Pathogenic Bacteria: In Vitro Study

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 546
Author(s):  
Nikola Puvača ◽  
Jovana Milenković ◽  
Tamara Galonja Galonja Coghill ◽  
Vojislava Bursić ◽  
Aleksandra Petrović ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Tea Tree ◽  
E Coli

The worldwide problem of infectious diseases has appeared in recent years, and antimicrobial agents are crucial in reducing disease emergence. Nevertheless, the development and distribution of multidrug-resistant (MDR) strains in pathogenic bacteria, such as Escherichia coli, Staphylococcus aureus, Salmonella Typhi and Citrobacter koseri, has become a major society health hazard. Essential oils could serve as a promising tool as a natural drug in fighting the problem with these bacteria. The current study aimed to investigate the antimicrobial effectiveness of tea tree (Melaleuca alternifolia (Maiden and Betche) Cheel), rosemary (Rosmarinus officinalis L.), eucalyptus (Eucalyptus obliqua L’Hér.), and lavender (Lavandula angustifolia Mill) essential oils. The antimicrobial properties of essential oils were screened against four pathogenic bacteria, E. coli, S. aureus, S. Tyhpi, and C. koseri, and two reference bacterial strains, while for the testing, the agar well diffusion method was used. Gas chromatography (GC) and gas chromatography–mass spectrometric (GC–MSD) analyses were performed on essential oils. The obtained results showed that M. alternifolia essential oil is the richest in terpinen-4-ol, R. officinalis and E. oblique essential oils in 1,8-cineole, and L. angustifolia essential oil in α-terpinyl acetate. In addition, the main bioactive compounds present in the essential oil of tea tree are rich in α-pinene (18.38%), limonene (7.55%) and γ-terpinene (14.01%). The essential oil of rosemary is rich in α-pinene (8.38%) and limonene (11.86%); eucalyptus essential oil has significant concentrations of α-pinene (12.60%), p-cymene (3.24%), limonene (3.87%), and γ-terpinene (7.37%), while the essential oil of lavender is rich in linalool (10.71%), linalool acetate (9.60%), α-terpinyl acetate (10.93%), and carbitol (13.05%) bioactive compounds, respectively. The obtained results from the in vitro study revealed that most of the essential oils exhibited antimicrobial properties. Among the tested essential oils, tea tree was discovered to demonstrate the strongest antimicrobial activity. The recorded MIC of S. Typhi was 6.2 mg/mL, 3.4 mg/mL of C. koseri, 3.1 mg/mL of E. coli, and 2.7 mg/mL of E. Coli ATCC 25922, compared to M. alternifolia. Similarly, only S. aureus ATCC 25923 showed antimicrobial activity towards R. officinalis (1.4 mg/mL), E. oblique (2.9 mg/mL), and L. angustifolia (2.1 mg/mL). Based on the obtained results, it is possible to conclude that tea tree essential oil might be used as an ecological antimicrobial in treating infectious diseases caused by the tested pathogens.

scholarly journals Influence of jasmonic acid on the growth and antimicrobial and antioxidant activities of Lavandula angustifolia Mill. propagated in vitro

2018 ◽  
Vol 30 (1) ◽  
pp. 3-13 ◽  
Author(s):  
Dominika Andrys ◽  
Danuta Kulpa ◽  
Monika Grzeszczuk ◽  
Bożena Białecka
Keyword(s):  
Antimicrobial Activity ◽  
Jasmonic Acid ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Growth Parameters ◽  
Antioxidant Properties ◽  
Antimicrobial Properties ◽  
Diffusion Method ◽  
Plant Weight

Abstract The aim of the study was to determine the influence of jasmonic acid added to culture medium on the growth of plants and antioxidant properties of dry plant material, as well as on the antimicrobial properties of essential oils produced by the narrow-leaved lavender. For plant propagation, MS media supplemented with JA at concentrations of 0.2-1.5 mg dm−3 were used. The use of the lower JA concentrations did not influenced the growth parameters measured, whereas at the higher concentrations (1 and 1.5 mg dm−3) JA caused growth inhibition and a decrease in plant weight. With increasing JA concentration, the number of secretory trichomes decreased. Addition of 0.5 mg dm−3 JA caused an increase in secretory trichome diameter on both the adaxial and abaxial surface of leaves (83.3 and 73.2 μm, respectively). The antioxidant activity of the lavender plants propagated on media with the addition of JA (regardless of the concentration used) was higher than that of the control plants. The plants from JA-supplemented media were used to isolate essential oils, the antimicrobial activity of which was tested using the disc diffusion method at the concentrations of 10 and 50%. All the oils tested exhibited activity towards Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans. The essential oils isolated from the plants propagated on the medium with 1 mg dm−3 JA were characterized by the highest antimicrobial activity against the majority of the tested microorganisms.

scholarly journals WCK 5222 (Cefepime-Zidebactam) Antimicrobial Activity against Clinical Isolates of Gram-Negative Bacteria Collected Worldwide in 2015

2017 ◽  
Vol 61 (5) ◽  
Author(s):  
Helio S. Sader ◽  
Mariana Castanheira ◽  
Michael Huband ◽  
Ronald N. Jones ◽  
Robert K. Flamm
Keyword(s):  
Antimicrobial Activity ◽  
Bacterial Infections ◽  
The United States ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Asia Pacific ◽  
Gram Negative ◽  
Content Type ◽  
Broth Microdilution Method

ABSTRACT WCK 5222 consists of cefepime combined with zidebactam, a bicyclo-acyl hydrazide β-lactam enhancer antibiotic with a dual action involving binding to Gram-negative bacterial PBP2 and β-lactamase inhibition. We evaluated the in vitro activity of cefepime-zidebactam against 7,876 contemporary (2015) clinical isolates of Enterobacteriaceae (n = 5,946), Pseudomonas aeruginosa (n = 1,291), and Acinetobacter spp. (n = 639) from the United States (n = 2,919), Europe (n = 3,004), the Asia-Pacific (n = 1,370), and Latin America (n = 583). The isolates were tested by a reference broth microdilution method for susceptibility against cefepime-zidebactam (1:1 and 2:1 ratios) and comparator agents. Cefepime-zidebactam was the most active compound tested against Enterobacteriaceae (MIC50/90, ≤0.03/0.12 μg/ml [1:1] and 0.06/0.25 μg/ml [2:1]; 99.9% of isolates were inhibited at ≤4 [1:1] and ≤8 μg/ml [2:1]). Cefepime-zidebactam was active against individual Enterobacteriaceae species (MIC50/90, ≤0.03 to 0.06/≤0.03 to 0.5 μg/ml [1:1]) and retained potent activity against carbapenem-resistant isolates (MIC50/90, 1/4 μg/ml; 99.3% of isolates were inhibited at ≤8 μg/ml [1:1]). Cefepime-zidebactam activity was consistent among geographic regions, and only one isolate showed MIC values of >8 μg/ml (1:1). Cefepime-zidebactam was also very active against P. aeruginosa with MIC50/90 values of 1/4 μg/ml, and 99.5% of isolates were inhibited at ≤8 μg/ml (1:1). The MIC values for cefepime-zidebactam at the 1:1 ratio were generally 2-fold lower than those for cefepime-zidebactam at the 2:1 ratio (MIC50/90, 2/8 μg/ml) and zidebactam alone (MIC50/90, 4/8 μg/ml). Against Acinetobacter spp., cefepime-zidebactam at 1:1 and 2:1 ratios (MIC50/90, 16/32 μg/ml for both) was 4-fold more active than cefepime or ceftazidime. Zidebactam exhibited potent in vitro antimicrobial activity against some organisms. These results support the clinical development of WCK 5222 for the treatment of Gram-negative bacterial infections, including those caused by multidrug-resistant isolates.

Chemical Composition and Antimicrobial Activity of the Essential Oils from Chloranthus japonicus Sieb. and Chloranthus multistachys Pei

2010 ◽  
Vol 65 (11-12) ◽  
pp. 660-666 ◽  
Author(s):  
Jie-fang Kang ◽  
Yuan Zhang ◽  
Yong-liang Du ◽  
Zhe-zhi Wang
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Disc Diffusion ◽  
Natural Sources ◽  
Pharmaceutical Industries ◽  
Inhibition Zones ◽  
Microbroth Dilution

We examined the composition and antimicrobial activity of two essential oils from Chloranthus japonicus Sieb. and Chloranthus multistachys Pei. GC-FID and GC-MS analyses identifi ed 48 and 39 compounds, which represented 95.56% and 94.58%, respectively, of all components in these oils. Of these, 28 compounds were common to both, with a relatively high amount of oxygenated monoterpenes (50.95% and 39.97%). Antimicrobial properties were evaluated in vitro via disc diffusion and microbroth dilution assays. Activities were strong against most tested microorganisms, with inhibition zones ranging from 8.1 to 22.2 mm. For both species, minimum values for inhibitory and bactericidal concentrations were 0.39 to 12.50 mg/mL and 0.78 to 50.00 mg/mL, respectively. These results suggest that these essential oils are potent natural sources of antimicrobial agents for the medicinal and pharmaceutical industries

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