Chemical Composition and Antimicrobial Activity of the Essential Oils from Chloranthus japonicus Sieb. and Chloranthus multistachys Pei

2010 ◽  
Vol 65 (11-12) ◽  
pp. 660-666 ◽  
Author(s):  
Jie-fang Kang ◽  
Yuan Zhang ◽  
Yong-liang Du ◽  
Zhe-zhi Wang
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Disc Diffusion ◽  
Natural Sources ◽  
Pharmaceutical Industries ◽  
Inhibition Zones ◽  
Microbroth Dilution

We examined the composition and antimicrobial activity of two essential oils from Chloranthus japonicus Sieb. and Chloranthus multistachys Pei. GC-FID and GC-MS analyses identifi ed 48 and 39 compounds, which represented 95.56% and 94.58%, respectively, of all components in these oils. Of these, 28 compounds were common to both, with a relatively high amount of oxygenated monoterpenes (50.95% and 39.97%). Antimicrobial properties were evaluated in vitro via disc diffusion and microbroth dilution assays. Activities were strong against most tested microorganisms, with inhibition zones ranging from 8.1 to 22.2 mm. For both species, minimum values for inhibitory and bactericidal concentrations were 0.39 to 12.50 mg/mL and 0.78 to 50.00 mg/mL, respectively. These results suggest that these essential oils are potent natural sources of antimicrobial agents for the medicinal and pharmaceutical industries

scholarly journals An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4383
Author(s):  
Barbara Lapinska ◽  
Aleksandra Szram ◽  
Beata Zarzycka ◽  
Janina Grzegorczyk ◽  
Louis Hardan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Resin Composite ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Diffusion Method ◽  
Oral Pathogens

Modifying the composition of dental restorative materials with antimicrobial agents might induce their antibacterial potential against cariogenic bacteria, e.g., S.mutans and L.acidophilus, as well as antifungal effect on C.albicans that are major oral pathogens. Essential oils (EOs) are widely known for antimicrobial activity and are successfully used in dental industry. The study aimed at evaluating antibacterial and antifungal activity of EOs and composite resin material (CR) modified with EO against oral pathogens. Ten EOs (i.e., anise, cinnamon, citronella, clove, geranium, lavender, limette, mint, rosemary thyme) were tested using agar diffusion method. Cinnamon and thyme EOs showed significantly highest antibacterial activity against S.mutans and L.acidophilus among all tested EOs. Anise and limette EOs showed no antibacterial activity against S.mutans. All tested EOs exhibited antifungal activity against C.albicans, whereas cinnamon EO showed significantly highest and limette EO significantly lowest activity. Next, 1, 2 or 5 µL of cinnamon EO was introduced into 2 g of CR and microbiologically tested. The modified CR showed higher antimicrobial activity in comparison to unmodified one. CR containing 2 µL of EO showed the best antimicrobial properties against S.mutans and C.albicans, while CR modified with 1 µL of EO showed the best antimicrobial properties against L.acidophilus.

scholarly journals Komposisi Minyak Atsiri dan Aktivitas Antimikroba Rimpang Temu Putih dan Jahe Gajah sebagai Fitobiotik Pakan Unggas

2019 ◽  
Vol 6 (2) ◽  
pp. 181
Author(s):  
Laila Nur Rohma ◽  
Laila Nur Rohma ◽  
Osfar Sjofjan ◽  
M. Halim Natsir
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Feed Additives ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Activity Test ◽  
Main Component

ABSTRAK                                                                        Imbuhan pakan unggas dapat berasal dari bahan herbal yang mengandung berbagai komponen aktif yang bermanfaat bagi pertumbuhan ternak.Temu putih dan jahe gajah dapat dimanfaatkan sebagai imbuhan pakan karena mengandung minyak atsiri yang dapat berperan sebagai agen antibakteri. Penelitian ini bertujuan untuk mengetahui komponen penyusun minyak atsiri dan aktivitas antimikroba pada rimpang temu putih dan jahe gajah. Penelitian dilakukan dengan percobaan in vitro menggunakan temu putih dan jahe gajah yang diolah menjadi bentuk ekstrak minyak atsiri temu putih dan jahe gajah sebagai materi uji komposisi penyusun minyak atsiri serta bentuktepung dan enkapsulasi sebagai materi uji aktivitas antimikroba. Komposisi minyak atsiri temu putih terdiri dari lima komponen penyusun dengan cis-1,7-octadien-3-yl acetat sebagai komponen utama. Komposisi minyak atsiri jahe gajah terdiri dari tujuh komponen dan benzene,1-(1,5-dimethyl-4-hexenyl)-4-methyl-(CAS) ar-curcumene sebagai komponen utama. Minyak atsiri yang terkandung pada temu putih dan jahe gajah mempunyai peran dalam menghambat mikroba. Uji komposisi penyusun minyak atsiri menggunakan alat GC-MS dan uji aktivitas antimikroba menggunakan metode disc diffusion dan. Hasil dari uji aktivitas antimikroba menunjukkan bahwa temu putih dan jahe gajah dalam bentuk tepung dan enkapsulasi memiliki perbedaan yang sangat nyata (P<0,01) terhadap aktivitas antimikroba pada bakteri asam laktat, Escherichia coli dan Salmonella sp. Campuran temu putih dan jahe gajah (1:1) menunjukkan kemampuan terbaik dalam menghambat pertumbuhan bakteri patogen dengan diameter zona hambat 5,70±0,14 mm  (Escherichia coli) dan 6,88±0,45 mm (Salmonella sp.).Kata Kunci : antimikroba, fitobiotik, jahe gajah, minyak atsiri, temu putihABSTRACTThe poultry feed additives can contain herbal ingredients that contain various beneficial components for livestock growth. White turmeric and giant ginger can be used as feed additives because they contain essential oils that can be used as antibacterial agents. This study aims to determine the constituent components of essential oils and antimicrobial activity in white turmeric and giant ginger rhizomes. The study was carried out by in vitro experiments using white turmeric and giant ginger which were processed into the form of essential oil extract as material for the composition of essential oils test, and powder and encapsulation form as antimicrobial activity test material. The composition of essential oils of white turmeric consists of five constituent components with cis-1,7-octadien-3-yl acetate as the main component. The composition of giant ginger essential oil consists of seven components with benzene, 1- (1,5-dimethyl-4-hexenyl) -4-methyl- (CAS) ar-curcumene as the main component. Essential oils contained in the white turmeric and giant ginger have a role in inhibiting microbes. The composition of the essential oil tested using GC-MS and the antimicrobial activity test used the disc diffusion method. The results of the antimicrobial activity test showed that white turmeric and giant ginger in powder and encapsulation form had significant differences (P <0.01) on antimicrobial activity in lactic acid bacteria, Escherichia coli and Salmonella sp. The mixture of white turmeric and giant ginger (1: 1) showed the best ability to inhibit the growth of pathogenic bacteria with inhibitory zone diameters of 5.70 ± 0.14 mm (Escherichia coli) and 6.88 ± 0.45 mm (Salmonella sp.).Keywords: antimicrobial, essential oil, giant ginger, phytobiotic, white turmeric

Evaluation of Bioactivity of Linalool-rich Essential Oils from Ocimum basilucum and Coriandrum sativum Varieties

2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Ahmet D. Duman ◽  
Isa Telci ◽  
Kenan S. Dayisoylu ◽  
Metin Digrak ◽  
İbrahim Demirtas ◽  
...  
Keyword(s):  
Essential Oils ◽  
Inhibitory Concentration ◽  
Antimicrobial Properties ◽  
Methyl Eugenol ◽  
Coriandrum Sativum ◽  
Disc Diffusion ◽  
Antimicrobial Effects ◽  
Minimal Inhibitory Concentrations ◽  
Test Organisms ◽  
Inhibition Zones

Essential oils from Ocimum basilicum L. and Coriandrum sativum L. varieties originating from Turkey were investigated for their antimicrobial properties. The antimicrobial effects of the oil varieties were evaluated by the disc diffusion and minimum inhibitory concentration (MIC) methods against eight bacteria and three fungi. The compositions of the essential oils were analyzed and identified by GC and GC-MS. O. basilicum, C. sativum var. macrocarpum and var. microcarpum oils revealed the presence of linalool (54.4%), eugenol (9.6%), methyl eugenol (7.6%); linalool (78.8%), γ-terpinene (6.0%), nerol acetate (3.5%); and linalool (90.6%), and nerol acetate (3.3%) as the major components, respectively. The oils exhibited antibacterial activity ranging from 1.25 to 10 μL disc−1 against the test organisms with inhibition zones of 9.5-39.0 mm and minimal inhibitory concentrations values in the range 0.5-≥1μL/L. Linalool, eugenol, and methyl eugenol at 1.25 μL disc−1 had antimicrobial effects on all microorganisms, giving inhibition zones ranging from 7 to 19 mm.

scholarly journals In vitro activity of hybrid lavender essential oils against multidrug resistant strains of Pseudomonas aeruginosa

2018 ◽  
Vol 12 (01) ◽  
pp. 009-014 ◽  
Author(s):  
Matthew Donadu ◽  
Donatella Usai ◽  
Antonio Pinna ◽  
Tiziana Porcu ◽  
Vittorio Mazzarello ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Cell Model ◽  
Antimicrobial Properties ◽  
The United States ◽  
Multidrug Resistant ◽  
New Antibiotics ◽  
Mediterranean Regions ◽  
A Cell

Introduction: Lavender is an evergreen shrub native to Northern Africa and other mountainous Mediterranean regions. It grows throughout Southern Europe, the United States, and Australia. Lavender essential oil has been used since ancient times and is known for its anti-inflammatory, antidepressant, antiseptic, antifungal and antimicrobial properties. Methodology: in this study, the antimicrobial activity of two Lavender essential oils (Lavanda sumian and Lavanda grosso) against 16 multidrug-resistant P. aeruginosa strains from clinical ocular samples taken from migrant patients has been investigated. The in vitro cytotoxic activity on human Wong-Kilbourne derivative (WKD) conjunctiva cells from healthy patients and nitric oxide synthase (NOS) activity on murine macrophage (J774.1A) were also evaluated. Results: L. sumian showed lower antimicrobial activity when compared to L. grosso. Both lavender oils tested had no cytotoxic effect at very low concentrations, mostly L. grosso. The essential oils extracted from L. sumian and L. grosso significantly reduced NOS in a cell model. Conclusion: Increase in drug resistance and lack of new antibiotics may encourage the development of natural antimicrobial treatments.

New Caffeic Acid Derivatives as Antimicrobial Agents: Design, Synthesis, Evaluation and Docking

2019 ◽  
Vol 19 (4) ◽  
pp. 292-304 ◽  
Author(s):  
Maia Merlani ◽  
Vakhtang Barbakadze ◽  
Lela Amiranashvili ◽  
Lali Gogilashvili ◽  
Vladimir Poroikov ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Caffeic Acid ◽  
Antimicrobial Agents ◽  
Biological Activities ◽  
Antimicrobial Properties ◽  
Synthesis Methods ◽  
Design Synthesis ◽  
Caffeic Acid Derivatives ◽  
Microdilution Method

Background: Phenolic acids (caffeic-, ferulic and p-coumaric acid) are widely distributed in the plant kingdom and exhibit broad spectrum of biological activities, including antimicrobial activity. Objective: The goal of this paper is the synthesis of some caffeic acid derivatives selected based on computer-aided predictions and evaluate their in vitro antimicrobial properties against Gram positive and Gram negative bacteria and also a series of fungi. Methods: In silico prediction of biological activity was used to identify the most promising structures for synthesis and biological testing, and the putative mechanisms of their antimicrobial action. The designed compounds were synthesized using classical organic synthesis methods. The antimicrobial activity was studied using microdilution method. Results: Twelve tested compounds have shown good antibacterial activity. Five out of twelve tested compounds appeared to be more active than the reference drugs ampicillin and streptomycin. Despite that all compounds exhibited good activity against all bacteria tested, the sensitivity of bacteria towards compounds in general was different. The evaluation of antifungal activity revealed that all compounds were more active than ketoconazole, while seven compounds (2, 3, 4, 5, 7, 8 and 12) appeared to be more active than bifonazole. Docking results indicate that gyrase inhibition is the putative mechanism of antibacterial action while the inhibition of 14α-demethylase may be responsible for antifungal action. Prediction of cytotoxicity by PROTOX showed that compounds are not toxic (LD50 1000-2000 mg/kg). Conclusion: Thirteen compounds, from which six are new ones, were synthesized, and twelve compounds were tested for antimicrobial activity. The studied compounds appeared to be promising potent and non-toxic antimicrobials, which could be considered as leads for new pharmaceutical agents.

scholarly journals Design, Synthesis and Biological Assessment of Thiazole Derivatives as Possible Antioxidant and Antimicrobial Agents

2021 ◽  
pp. 24-32
Author(s):  
. Isha ◽  
Neetu Sachan
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Therapeutic Potential ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Molecular Structures ◽  
Biological Assessment ◽  
Thiazole Derivatives ◽  
Antioxidant And Antimicrobial Activity

Aims: To synthesize thiazole derivatives and evaluate their therapeutic potential to continue our quest for new antibacterial and antioxidant drugs. Place and Duration of Study: Institute of Pharmaceutical Research, GLA University, Mathura, Uttar Pradesh, between January 2021 and July 2021. Methodology: The target compounds in this investigation were synthesized in the search for new molecules having antioxidant and antimicrobial activity. Physicochemical and spectroanalytical studies validated the derivatives molecular structures. Antioxidant and antimicrobial properties of the synthesized molecules were evaluated in vitro using the DPPH and tube dilution methods, respectively. Results: The majority of the synthesized derivatives displayed antioxidant and antimicrobial activity. The efficacy of the derivatives varied based upon the substituent. Compound 7c exhibited significant antioxidant and antibacterial activity, according to the results of the study. Conclusion: Our results showed the antioxidant and antibacterial properties of novel thiazole compounds, implying the probability of their utilization in the development of new therapeutics.

scholarly journals Antimicrobial activity of a novel Spanish propolis against planktonic and sessile oral Streptococcus spp

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. Luisa Navarro-Pérez ◽  
Virginia Vadillo-Rodríguez ◽  
Irene Fernández-Babiano ◽  
Ciro Pérez-Giraldo ◽  
M. Coronada Fernández-Calderón
Keyword(s):  
Antimicrobial Activity ◽  
Biofilm Formation ◽  
Bacterial Resistance ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Oral Streptococcus ◽  
Ancient Times ◽  
Ethanolic Extract Of Propolis

AbstractIncreased bacterial resistance to traditional antimicrobial agents has prompted the use of natural products with antimicrobial properties such as propolis, extensively employed since ancient times. However, the chemical composition of propolis extracts is extremely complex and has been shown to vary depending on the region and season of collection, due to variations in the flora from which the pharmacological substances are obtained, being therefore essential for their antimicrobial activity to be checked before use. For this purpose, we evaluate the in vitro antimicrobial and anti-biofilm activity of a new and promising Spanish ethanolic extract of propolis (SEEP) on Streptococcus mutans and Streptococcus sanguinis, responsible, as dominant ‘pioneer’ species, for dental plaque. Results reveal that S. sanguinis is more sensitive to SEEP, slowing and retarding its growth considerably with lower concentrations than those needed to produce the same effect in S. mutans. SEEP presents concentration- and time-dependent killing activity and, furthermore, some of the subinhibitory concentrations employed increased biofilm formation even when bacterial growth decreased. Mono and dual-species biofilms were also inhibited by SEEP. Findings obtained clearly show the relevance of using biofilm and subinhibitory concentration models to determine optimal treatment concentrations.

scholarly journals Synthesis, characterization, synergistic antimicrobial properties and molecular docking of sugar modified uridine derivatives

2021 ◽  
Vol 32 (1) ◽  
pp. 6-21
Author(s):  
Jannatul Maowa ◽  
Asraful Alam ◽  
Kazi M. Rana ◽  
Sujan Dey ◽  
Anowar Hosen ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Molecular Docking ◽  
Density Functional ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Docking Study ◽  
Bacterial Strains ◽  
Molecular Docking Study ◽  
Antibacterial And Antifungal Activities

Abstract Nucleosides and their analogues are an important, well-established class of clinically useful medicinal agents that exhibit antiviral and anticancer activity. Thus, our research group has focused on the synthesis of new nucleoside derivatives that could be tested for their broad-spectrum biological activity. In this study, two new series of nucleoside derivatives were synthesized from uridine (1) through facile two-step reactions using the direct acylation method, affording 5’-O-acyl uridine derivatives in good yields. The isolated uridine analogs were further transformed into two series of 2’,3’-di-O-acyl derivatives bearing a wide variety of functionalities in a single molecular framework to evaluate their antimicrobial activity. The new synthesized compounds were characterized through physicochemical, elemental and spectroscopic analysis, and all were screened for their in vitro antimicrobial activity against selected human and plant pathogenic strains. The test compounds revealed moderate to good antibacterial and antifungal activities and were more effective against fungal phytopathogens than against bacterial strains, while many of them exhibited better antimicrobial activity than standard antibiotics. Minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) tests against all microorganisms were also conducted for five compounds based on their activity (6, 11, 13, 16, and 17). In addition, all the derivatives were optimized using density functional theory (DFT) B3LYP/6-31g+(d,p) calculations to elucidate their thermal and molecular orbital properties. A molecular docking study was performed using the human protein 5WS1 to predict their binding affinity and modes, and ADMET and SwissADME calculations confirmed the improved pharmacokinetic properties of the compounds. Besides, structure–activity relationship (SAR), thermogravimetric analysis (TGA), and X-ray diffraction (XRD) studies were also performed. Thus, the improvement of the bioactivity of these compounds is expected to significantly contribute to the design of more antimicrobial agents for therapeutic use in the future.

scholarly journals Study of the Antimicrobial Potential of Bacteria found in Natural Resources

2021 ◽  
Author(s):  
Svetlana Noskova ◽  
Svetlana Ivanova ◽  
Alexander Prosekov ◽  
Lyubov Dyshlyuk ◽  
Elena Ulrikh ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Natural Resources ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Natural Sources ◽  
E Coli ◽  
Antagonistic Microorganisms ◽  
Its Nrdna

Bacteriocins are of great interest as potential antimicrobial agents against various types of bacteria, fungi, and viruses. Isolates of microorganisms derived from natural sources were used in the current study, including lactic acid bacteria and other antagonistic microorganisms. The species of the microorganisms were determined using 16S rDNA and ITS nrDNA analyses. E. coli, S. enterica, S. aureus, P. aeruginosa, B. mycoides, A. faecalis, P. vulgaris, S. flexneri , L. monocytogenes, C. albicans, A. flavus, and P. citrinum were used as pathogenic and opportunistic strains. It was found that 11 strains of antagonistic microorganisms have significant antimicrobial activity against all pathogenic and opportunistic microorganisms. The antimicrobial properties of these microorganisms are currently under study.

scholarly journals In vitro activity of essential oils against Pseudomonas aeruginosa isolated from infected hip implants

2018 ◽  
Vol 12 (11) ◽  
pp. 996-1001 ◽  
Author(s):  
Veronica Amorese ◽  
Matthew Donadu ◽  
Donatella Usai ◽  
Angelino Sanna ◽  
Fabio Milia ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Antimicrobial Properties ◽  
Multidrug Resistant ◽  
Thymus Vulgaris ◽  
Hip Implants ◽  
Melaleuca Alternifolia ◽  
New Antibiotics ◽  
Normal Human

Introduction: Essential oils have been used since ancient times and are known for their anti-inflammatory, anti-depressive, antiseptic, antifungal and antimicrobial properties. Methodology: in this study the antimicrobial activity of two essential oils from Melaleuca alternifolia and Thymus vulgaris–red thyme geraniol was tested against 16 multidrug-resistant P. aeruginosa strains from infected hip implants as well as the “in vitro” cytotoxic activity on normal human Wong-Kilbourne derivative (WKD) cells. Results: Thymus vulgaris–red thyme geraniol showed lower antimicrobial activity when compared to Melaleuca alternifolia. All tested oils were cytotoxic at concentrations lower than 0.12%. Conclusion: Increase in drug resistance and lack of new antibiotics may encourage the development of natural treatments together with higher concern on environmental issues and natural lifestyle.

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