scholarly journals High Circulating Sclerostin Level as Osteoporosis Risk Factor in Male Geriatric Population at Sanglah Hospital Bali

2021 ◽  
Vol 9 (B) ◽  
pp. 1032-1036
Author(s):  
Sandra Suryarini ◽  
RA. Tuty Kuswardhani ◽  
I. Gusti Lanang Ngurah Agung Artha Wiguna
Keyword(s):  
Risk Factor ◽  
Serum Level ◽  
Bone Mass ◽  
Multivariate Logistic Regression Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Case Group ◽  
Mineral Density ◽  
Geriatric Population ◽  
Structural Deterioration ◽  
Sclerostin Level

BACKGROUND: Osteoporosis is a disease characterized by low bone mass and structural deterioration of bone tissue, leading to bone fragility. The development of biomolecular world found Wnt/β-catenin signaling pathway may plays an important role in bone mass regulation. Osteoporosis in geriatric population remains one of global health problems and typically thought of as a disease impacting women, but recently increasing attention is being paid to osteoporosis in males. Osteoporosis in male accounts for higher morbidity and mortality compare to woman population. The association between sclerostin serum and risk for osteoporosis in male geriatric will be described as follows. METHODS: This study is a case–control study with a total 54 samples of male geriatrics, divided into 27 non- osteoporosis subjects and 27 osteoporosis subjects (age ≥60 years old). Diagnosis of osteoporosis was defined according to the WHO criteria based on bone mineral density. All participants were scanned on a GE lunar prodigy bone densitometer. Sclerostin serum level was measured using enzyme-linked immunosorbent assay (ELISA). RESULTS: The average age from total 54 samples in case group was 69.81 ± 6.5 years old and control 69.41 ± 5.97 years old. Cutoff value based on receiver operating characteristic curve for sclerostin serum level was 302.5 pg/mL where the sensitivity and specificity for developing osteoporosis in male geriatrics were 59.3% and 81.5%, respectively. Male geriatrics with sclerostin serum ≥302.5 pg/mL is 6.4 times more likely to developed osteoporosis than those with sclerostin serum <302.5 pg/mL (OR = 6.4; p = 0.0020; CI 95% = 1.856–22.068). Multivariate logistic regression analysis after controlling other variables such as bone mass index, age, smoking status, alcohol consumption, physical activity, sun exposure, and type II diabetes mellitus showed that high sclerostin level was an independent susceptibility factors for osteoporosis in male geriatrics population (p = 0.001). CONCLUSIONS: This study showed that high circulating sclerostin serum (≥302.5 pg/mL) was risk factor for developing osteoporosis in male geriatrics.

scholarly journals Case-Control Study to Assess the Association between Epilepsy and Toxocara Infection/Exposure

2021 ◽  
Vol 9 (10) ◽  
pp. 2091
Author(s):  
Ali Alizadeh Khatir ◽  
Mahdi Sepidarkish ◽  
Mohammad Reza Rajabalizadeh ◽  
Solmaz Alizadeh Moghaddam ◽  
Saeed Aghapour ◽  
...  
Keyword(s):  
Risk Factor ◽  
Case Control Study ◽  
Serum Antibody ◽  
Multivariate Logistic Regression Analysis ◽  
Case Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Subjects ◽  
Epileptic Patients ◽  
Incident Cases ◽  
Control Study

Although causes and etiology of epilepsy are mostly obscure, some zoonotic parasites, such as Toxocara species, have been proposed as a risk factor for this disease. Here, we conducted an age-matched case-control study to evaluate whether there is an association between epilepsy and the presence of serum antibodies to Toxocara in incident cases. We included 94 idiopathic epileptic patients as cases, and—from the same geographical region—88 people with no own history of epilepsy or neurological disease as control subjects. Epilepsy was confirmed by a physician using the International League Against Epilepsy (ILAE) definition. All participants were screened for the anti-Toxocara IgG serum antibody by enzyme-linked immunosorbent assay (ELISA). Univariate and mutltivariate statistical analyses were applied to calculate the crude and adjusted odds ratios (OR) and 95% confidence intervals (CIs). Anti-Toxocara serum antibody was detected in 37 epileptic patients and in 23 control subjects, giving respective seroprevalences of 39.3% (95% CI, 29.4–49.9%) and 26.1% (95% CI, 17.3–36.5%), respectively. Adjusted multivariate logistic regression analysis estimated an OR of 2.38 (95% CI, 1.25–4.63), indicating a significant association between epilepsy and Toxocara seropositivity. There was also a significant association between seropositivity to Toxocara and partial (OR, 2.60; 95% CI, 1.14–6.04) or generalized (OR, 2.17; 95% CI, 1.09–4.40%) seizures. Findings from the present study of incident epileptic cases support previous studies proposing that Toxocara infection/exposure is a risk factor for epilepsy. However, further well-designed population-based surveys and mechanistic/experimental studies in animal models are required to better understand the reason(s) for this association.

scholarly journals Influence of Age at Menarche on Forearm Bone Microstructure in Healthy Young Women

2008 ◽  
Vol 93 (7) ◽  
pp. 2594-2601 ◽  
Author(s):  
Thierry Chevalley ◽  
Jean-Philippe Bonjour ◽  
Serge Ferrari ◽  
Rene Rizzoli
Keyword(s):  
Bone Mineral Density ◽  
Risk Factor ◽  
Bone Mass ◽  
Bone Mineral ◽  
Peak Bone Mass ◽  
Adult Women ◽  
Mineral Density ◽  
T Score ◽  
Estrogen Exposure ◽  
Volumetric Bmd

Abstract Background: Shorter estrogen exposure from puberty onset to peak bone mass attainment may explain how late menarche is a risk factor for osteoporosis. The influence of menarcheal age (MENA) on peak bone mass, cortical, and trabecular microstructure was studied in 124 healthy women aged 20.4 ± 0.6 (sd) yr. Methods: At distal radius, areal bone mineral density (aBMD) was measured by dual-energy x-ray absorptiometry, and volumetric bone mineral density (BMD) and microstructure were measured by high-resolution peripheral computerized tomography, including: total, cortical, and trabecular volumetric BMD and fraction; trabecular number, thickness, and spacing; cortical thickness (CTh); and cross-sectional area (CSA). Results: Median MENA was 12.9 yr. Mean aBMD T score of the whole cohort was slightly positive. aBMD was inversely correlated to MENA for total radius (R = −0.21; P = 0.018), diaphysis (R = −0.18; P = 0.043), and metaphysis (R = −0.19; P = 0.031). Subjects with MENA more than the median [LATER: 14.0 ± 0.7 (±sd) yr] had lower aBMD than those with MENA less than the median (EARLIER: 12.1 ± 0.7 yr) in total radius (P = 0.026), diaphysis (P = 0.042), and metaphysis (P = 0.046). LATER vs. EARLIER displayed lower total volumetric BMD (315 ± 54 vs. 341 ± 56 mg HA/cm3; P = 0.010), cortical volumetric BMD (874 ± 49 vs. 901 ± 44 mg HA/cm3; P = 0.003), and CTh (774 ± 170 vs. 849 ± 191 μm; P = 0.023). CTh was inversely related to CSA (R = −0.46; P &lt; 0.001). In LATER reduced CTh was associated with 5% increased CSA. Conclusions: In healthy young adult women, a 1.9-yr difference in mean MENA was associated with lower radial aBMD T score, lower CTh without reduced CSA, a finding compatible with less endocortical accrual. It may explain how late menarche is a risk factor for forearm osteoporosis.

scholarly journals The Relation between Bone Mineral Density, Insulin-Like Growth Factor I, Lipoprotein (a), Body Composition, and Muscle Strength in Adolescent Males

1999 ◽  
Vol 84 (9) ◽  
pp. 3025-3029 ◽  
Author(s):  
Kim Thorsen ◽  
Peter Nordström ◽  
Ronny Lorentzon ◽  
Gösta H. Dahlén
Keyword(s):  
Physical Activity ◽  
Bone Mineral Density ◽  
Risk Factor ◽  
Muscle Strength ◽  
Bone Mass ◽  
Peak Bone Mass ◽  
Mineral Density ◽  
Factor I ◽  
Igf I ◽  
Total Body

Osteoporosis is the most common metabolic bone disease. A low peak bone mass is regarded a risk factor for osteoporosis. Heredity, physical activity, and nutrition are regarded important measures for the observed variance in peak bone mass. Lp(a) lipoprotein is a well-known risk factor for atherosclerosis. Serum insulin-like growth factor I (IGF-I) has been found to be increased in males with early cardiovascular disease. In this study, we evaluated the association between bone mass, body constitution, muscle strength, Lp(a), and IGF-I in 47 Caucasian male adolescents (mean age, 16.9 yr). Bone mineral density (BMD) and body composition were measured by dual x-ray absorptiometry, muscle strength of thigh using an isokinetic dynamometer, IGF-I by RIA, and Lp(a) by enzyme-linked immunosorbent assay. IGF-I was only associated with Lp(a) (r = 0.38, P &lt; 0.01). Lp(a) was related to total body (r= 0.40, P &lt; 0.01), skull (r = 0.45, P &lt; 0.01), and femoral neck BMD (r = 0.44, P &lt; 0.01). Lp(a) was also related to fat mass (r = 0.34, P &lt; 0.05) and muscle strength (r = 0.30–0.42, P &lt; 0.05). After multiple regression and principal component (PC) analysis, the so-called PC body size (weight, fat mass, lean body mass, and muscle strength) was the most significant predictor of BMD (β = 0.28–0.51, P &lt; 0.05–0.01), followed by the so-called PC physical activity (β = 0.28–0.38, P &lt; 0.05–0.01, weight-bearing locations). However, the PC analysis confirmed that Lp(a) was an independent predictor of total body, skull, and femoral neck BMD (β = 0.33–0.36, P &lt; 0.01). The present investigation confirms that BMD, body size, and muscle strength are closely related and that the level of physical activity is a major determinant of BMD. However, the positive relation of Lp(a), a major risk factor for cardiovascular disease, to BMD has not previously been described. The importance of this observation has to be further investigated.

scholarly journals High plasma dopamine level as a risk factor for atopic dermatitis

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Made Swastika Adiguna ◽  
Made Wardhana ◽  
Ermon Naftali Limbara
Keyword(s):  
Atopic Dermatitis ◽  
Risk Factor ◽  
Inflammatory Response ◽  
Venous Blood ◽  
High Plasma ◽  
Matched Pair ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Case Group ◽  
Dopamine Level

Background: Dopamine is responsible for inflammatory response and plays a role in the skin immune system by modulating T-cells, dendritic cells, and keratinocytes which increases skin inflammatory response in atopic dermatitis (AD). Elevation of dopamine level will affect IL-6, IL-8, IL-23, Th-17, and TNF-α, which promotes keratinocyte proliferation and differentiation, infiltration of inflammatory cells, angiogenesis, vasodilation, and skin barrier disruption on AD.Objective: This study aimed to establish whether the increase of plasma dopamine level contributes to a risk factor for AD occurrence.Methods: This is a matched-pair case-control observational analytical study which involves patients with AD and without AD as control. Samples were taken using a consecutive sampling method which fulfilled inclusion and exclusion criteria, matched for gender and age. Plasma dopamine level was measured from venous blood and processed using enzyme-linked immunosorbent assay (ELISA) method. The collected data were then analysed using SPSS version 20.0 with Pearson chi-square test for the odds ratio.Results: A total of 30 samples with AD (case group) and 30 samples without AD (control group) involved in this study. This study proves that plasma dopamine levels in the case group were significantly higher than the control group (p<0.05). Odds ratio for plasma dopamine was 42.2 (95%CI: 9.5-187.2, p < 0.001).Conclusion: This study concludes that high plasma dopamine level is a risk factor for AD.

scholarly journals Serum Level of Anti-Toxoplasma Gondii IgG among the Individuals with/without Migraine

2020 ◽  
Author(s):  
Niloufar Jouyani ◽  
Mohammad Taghi Ahady ◽  
Vahid Abbasi
Keyword(s):  
Toxoplasma Gondii ◽  
Serum Level ◽  
Pain Syndrome ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Case Group ◽  
Chi Square ◽  
Elisa Technique ◽  
The Mean ◽  
Age Range

Introduction: More than 500 million people throughout the world are infected with Toxoplasma gondii. On the other hand, migraine is known as the most common pain syndrome. The aim of this study was to detect anti-Toxoplasma gondii IgG in the serum of individuals with and without migraine. Methods: In this descriptive-analytical study, 50 person (7 male & 43 female, in the age range of 20-60 years) with history and symptoms of migraine (case group), and 50 individuals (7 male & 43 female, in the age range of 20-60 years) without migraine (control group) were selected randomly. Blood samples (5 ml) were collected from all the selected people, and the serum level of anti-Toxoplasma gondii IgG were determined using Enzyme-Linked Immunosorbent Assay (ELISA) technique. In this test, 10 IU/ml of anti-Toxoplasma gondii IgG was considered as the minimum titer. For statistical analysis SPSS Inc, Chicago, IL; Version 18 software and -Chi-square and t-tests were used. Results: 38% of the patients with migraine and 32% of the control group had anti-Toxoplasma gondii IgG above 10 IU/ml. The mean amount of anti-T. gondii IgG in the serums of case group was 173.42 IU/ml, while it was 68.25 IU/ml in the control group, the mean amount of Toxo-IgG in migraine positive group was 2.5 time higher than the amount in the control group (p<0.05). Conclusion: According to the results of this study it is concluded that the serum level of anti-Toxoplasma gondii IgG in migraine positive persons is significantly higher than the migraine negative individuals. Therefore, it is recommended that the patients disordered with migraine be tested for chronic toxoplasmosis.

scholarly journals Urinary Metabolic Profiling via LC-MS/MS Reveals Impact of Bovine Lactoferrin on Bone Formation in Growing SD Rats

Nutrients ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1116 ◽  
Author(s):  
Yan Xu ◽  
Tianyu Zhao ◽  
Haowei Ren ◽  
Yindan Xie ◽  
Jingjing An ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Bone Formation ◽  
Bone Mass ◽  
Metabolic Profiling ◽  
Fatty Acid Biosynthesis ◽  
Urinary Metabolites ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Lactoferrin ◽  
Mineral Density ◽  
Sd Rats

Lactoferrin (LF) exerts a promoting bone health function. The effects of LF on bone formation at the metabolic level have been less explored. Urinary metabolic profiling of growing Sprague-Dawley (SD) rats LF-supplemented (1000 mg/kg bw) for four weeks were explored by Liquid chromatography–tandem mass spectrometry (LC-MS/MS). The serum markers of bone formation and bone resorption, the bone mass, and the osteogenesis markers of femur were measured by an enzyme-linked immunosorbent assay, micro-computerized tomography, and immunohistochemistry, respectively. Compared with the control, LF supplementation improved bone formation (p < 0.05), reduced bone resorption (p < 0.05), enhanced femoral bone mineral density and microarchitecture (p < 0.05), and upregulated osteocalcin, osterix, and Runx-2 expression (p < 0.05) of femur. LF upregulated 69 urinary metabolites. KEGG and pathway enrichment analyses of those urinary metabolites, and the Person’s correlation analyses among those urinary metabolites and bone status revealed that LF impacted on bone formation via regulatory comprehensive pathways including taurine and hypotaurine metabolism, arginine and proline metabolism, cyanoamino acid metabolism, nitrogen metabolism, nicotinate and nicotinamide metabolism, and fatty acid biosynthesis. The present study indicated the metabolomics is a useful and practical tool to elucidate the mechanisms by which LF augments bone mass formation in growing animals.

scholarly journals Changes of Serum TNF-α in Acute Pancreatitis Patients and Its Clinical Significance

2015 ◽  
Vol 4 (4) ◽  
pp. 33
Author(s):  
Wenrui Yang
Keyword(s):  
Acute Pancreatitis ◽  
Serum Level ◽  
Clinical Significance ◽  
Severity Of Illness ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Case Group ◽  
Tnf Α ◽  
Α Level

<p><strong>Objective: </strong>The aimed of this study is to investigate the changes in serum levels of tumor necrosis factor (TNF-α) in the patients with acute pancreatitis treated with octreotide and its clinical significance. <strong>Method: </strong>Total of 65 patients of acute pancreatitis were selected as a case study, in which 30 patients with mild acute pancreatitis (MAP) and 35 severe acute pancreatitis (SAP) patients were treated with octreotide. 60 healthy subjects as control group and 65 case group was subjected to performed double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect the serum levels of TNF-α. <strong>Results</strong><strong>: </strong>The serum TNF-α level in the case group was (12.67 ± 3.45) pg/mL and the control group was (1.56 ± 0.57) pg/mL. Case group was significantly higher than control group (<em>p</em> &lt; 0.05). Serum level of acute pancreatitis (AP) before treatment was (8.96 ± 2.12) pg/mL. After treatment, SAP group was (17.34 + 4.56) pg/mL, MAP group was significantly lower than SAP group, and the difference was statistically significant (<em>p</em> &lt; 0.05). <strong>Conclusion: </strong>The serum levels of TNF-α in patient with acute pancreatitis were significantly higher than those of normal healthy people, and their serum level was closely related to the severity of illness.</p>

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