Vitamin E modulates lung oxidative stress, serum copper, zinc, and iron levels in rats with pulmonary contusion

1 The concentration of serum malondialdehyde (MDA) was measured as the index of lipid peroxidation in female epileptics with phenytoin (PHT) monotherapy. Sera from 20 female epileptics with PHT monotherapy, 12 female epileptics without anticonvulsant therapy and 20 female healthy controls were sampled. The levels of serum copper (S-Cu), serum zinc (S-Zn), copper/zinc superoxide dismutase (CuZn-SOD), and reduced glutathione (GSH) were analyzed as inter active factors of the oxidative stress. 2 For the female epileptics with PHT monotherapy, serum MDA concentration (2.6 ± 0.7 μM vs control 1.8 ± 0.6 μM, P < 0.05), CuZn-SOD activity (178.2 ± 63.5 U/dL vs control 97.1 ± 36.4 U/dL, P < 0.01), and S-Cu content (126.2 ± 36.1 μg/dL vs control 98.4 ± 16.7 μg/dL, P < 0.05) were significantly in creased, but GSH level (27.5±6.8 μM vs control 32.2 ± 5.7 μM, P < 0.05) was significantly decreased. The level of serum MDA was associated with the elevation of CuZn-SOD activity ( r=0.54, P < 0.05) and S-Cu content ( r=0.44, P < 0.05) in all the samples collected from epileptics and controls. However, there were no significant differences in all the above parameters between the female epileptics without anticonvulsant therapy and healthy controls. 3 These results indicated that oxidative stress was enhanced in the female epileptics with PHT-mono therapy. Apart from the reactive PHT intermediate, the abnormal metabolism of S-Cu, CuZn-SOD, and GSH was highly involved in the PHT-mediated toxicity. Supplement of GSH, modification of CuZn-SOD enzyme activity and reduction of the absorption of copper may prevent the incidence of fetal hydantoin syndrome during pregnancy.

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Estimation of Trace Elements in Gingival Crevicular Fluid and Serum-Comparative Study in Healthy and Periodontitis

International Journal of Pharmaceutical and Clinical Research ◽

10.25258/ijpcr.v9i5.8599 ◽

2017 ◽

Vol 9 (5) ◽

Author(s):

Bhagavathami Meenaksh ◽

Chitraa R. Chandran ◽

Aravindhan Thathchari Ranganathan ◽

Kavindapadi Venkateswaran Rama ◽

Valarmathy Srinivasan

Keyword(s):

Trace Elements ◽

Inductively Coupled Plasma ◽

Gingival Crevicular Fluid ◽

Serum Zinc ◽

Cross Sectional Study ◽

Serum Copper ◽

Cross Sectional ◽

Inductively Coupled ◽

Significant Difference ◽

Copper Zinc

Background: This study aims to determine and compare the levels of trace elements copper, zinc, selenium and chromium in GCF and serum of patients with periodontitis and healthy individuals. Methods: This cross sectional study includes 24 study subjects recruited from the patients reporting to the Department of Periodontics , Tagore Dental College Chennai. All the selected patients were subjected to a clinical examination done by a single examiner. The estimation of trace elements Copper, Zinc, Selenium and Chromium in GCF and serum is performed using Perkin Elmer optima 5300 Inductively Coupled Plasma Emission Spectrometer (ICPOES). Results: GCF and serum copper levels showed no significant difference in both periodontitis and healthy groups . Selenium levels tend to be the same in both groups. Serum zinc levels are more in periodontitis patients than healthy subjects (p less than 0.01). GCF chromium levels are found to be more in patients with periodontitis than healthy. Conclusions : More research is therefore needed to monitor the role of these trace elements C with an increased sample size to ascertain whether they are associated with a reduced risk of periodontitis.

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Nonalcoholic Fatty Liver Disease and Vitamin E - A Promising Relationship?

Revista de Chimie ◽

10.37358/rc.19.1.6855 ◽

2019 ◽

Vol 70 (1) ◽

pp. 78-83

Author(s):

Alexandra Totan ◽

Daniela Gabriela Balan ◽

Daniela Miricescu ◽

Radu Radulescu ◽

Iulia Ioana Stanescu ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Therapeutic Potential ◽

Plga Nanoparticles ◽

Diet Group ◽

Normal Diet ◽

Oxidative Stress Biomarkers ◽

Promising Alternative ◽

Nonalcoholic Fatty Liver ◽

High Caloric Diet

Oxidative stress (OS) plays an important role in NAFLD molecular mechanism. Nanoencapsulation represents a novel strategy to enhance therapeutic potential of conventional drugs. Our study analyses the encapsulated vitamin E effect on lipid metabolism and oxidative stress biomarkers in NAFLD rats. Animals were divided into 3 groups : G1 - the normal diet group; G2- the high caloric diet group ; G3 - high-caloric diet group receiving PLGA-vit E, 50 mg / kg. Serum advanced human oxidative protein (AOPP), total antioxidant capacity (TAC) and vitamin E were analysed using ELISA technique. Our results showed significant increase of G2 GPT, ALP, GGT, TG, glucose, TC and AOPP, versus G1 ( P [ 0.05) and a significant decrease of G2 serum TAC and vitamin E versus G1 results ( p = 0.01 and 0.01). Vitamin E nanoparticles (G3) caused a significant increase of TAC and significant decrease of serum AOPP, versus G2 (p [ 0.01). Results showed a significant reduction of GPT, GGT, ALP, TG and total cholesterol ( p [0.05) in G3 versus G2. PLGA nanoparticles should be considered an attractive and promising alternative to improve the bioavailability and biological activity of vitaminE.

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Effect of Trimeric Myricetin Rhamnoside (TMR) in Carrageenan-induced Inflammation and Caecal Ligation and Puncture-induced Lung Oxidative Stress in Mice

Phytotherapy Research ◽

10.1002/ptr.5446 ◽

2015 ◽

Vol 29 (11) ◽

pp. 1798-1805 ◽

Cited By ~ 11

Author(s):

Najeeb Latief ◽

Shikha Anand ◽

Madhu Cholenahalli Lingaraju ◽

Venkanna Balaganur ◽

Nitya Nand Pathak ◽

...

Keyword(s):

Oxidative Stress ◽

Caecal Ligation And Puncture ◽

Lung Oxidative Stress ◽

Caecal Ligation

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Dietary Copper/Zinc Ratio and Type 2 Diabetes Risk in Women: The E3N Cohort Study

Nutrients ◽

10.3390/nu13082502 ◽

2021 ◽

Vol 13 (8) ◽

pp. 2502

Author(s):

Nasser Laouali ◽

Conor-James MacDonald ◽

Sanam Shah ◽

Douae El Fatouhi ◽

Francesca Romana Mancini ◽

...

Keyword(s):

Type 2 Diabetes ◽

Cohort Study ◽

Important Determinant ◽

Epidemiological Data ◽

Serum Copper ◽

Obese Women ◽

Dietary Copper ◽

Zinc Intake ◽

Copper Zinc

The serum copper (Cu) to zinc (Zn) ratio could be an important determinant of Type 2 diabetes (T2D) risk, but prospective epidemiological data are scarce. We aimed to investigate the association between T2D incidence and the dietary Cu/Zn ratio. A total of 70,991 women from the E3N cohort study were followed for 20 years. The intakes of copper and zinc were estimated at baseline using a validated food frequency questionnaire. We identified and validated 3292 incident T2D cases. Spline analysis showed that a Cu/Zn ratio < 0.55 was associated with a lower risk of T2D. Subgroup analyses comparing women in the highest versus the lowest quintile of Cu/Zn ratio showed the same pattern of association for obese women and those with zinc intake ≥8 mg/day. However, for women with zinc intake <8 mg/day, higher Cu/Zn ratio appeared to be associated with higher T2D risk. Our findings suggest that a lower dietary Cu/Zn ratio is associated with a lower T2D risk, especially among obese women and women with zinc intake >8 mg/day. Further studies are warranted to validate our results.

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Associations between vitamin E, oxidative stress markers, total homocysteine levels, and physical activity or cognitive capacity in older adults

Scientific Reports ◽

10.1038/s41598-021-92076-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Ahmad H. Alghadir ◽

Sami A. Gabr ◽

Shahnawaz Anwer ◽

Heng Li

Keyword(s):

Oxidative Stress ◽

Physical Activity ◽

Nitric Oxide ◽

Older Adults ◽

Vitamin E ◽

Cognitive Capacity ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Total Antioxidant ◽

Total Homocysteine

AbstractThis study examined the associations between vitamin E, oxidative stress markers, total homocysteine levels, and physical activity or cognitive capacity in older adults. One hundred and six older adults (62 men, 44 women) within the age range of 56–81 years participated. The Global Physical Activity Questionnaire and the Loewenstein Occupational Therapy Cognitive Assessment were used to assess physical activity and cognitive function, respectively. Vitamin E (e.g., α-tocopherol and γ-tocopherol), oxidative stress markers (e.g., total antioxidant capacity and nitric oxide), and total homocysteine were estimated. There were significant associations between physical activity (high versus moderate versus poor) and all biomarkers (all p = 0.000, and p = 0.010 for γ-tocopherol). While total homocysteine and total antioxidant capacity were significantly associated with cognitive capacity (p = 0.000), vitamin E levels (e.g., α-tocopherol and γ-tocopherol) and nitric oxide (p = 0.354, 0.103 and 0.060, respectively) were not related to cognitive capacity in older adults. This study concludes that physical activity was associated with Vitamin E, oxidative stress markers, total homocysteine, and cognitive capacity in older adults. Although cognitive capacity was associated with total homocysteine and total antioxidant capacity, it was unrelated to vitamin E levels and nitric oxide in older adults.

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Biological variation and reference change value data for serum copper, zinc and selenium in Turkish adult population

Turkish Journal of Biochemistry ◽

10.1515/tjb-2020-0298 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Ceylan Bal ◽

Serpil Erdogan ◽

Gamze Gök ◽

Cemil Nural ◽

Betül Özbek ◽

...

Keyword(s):

Adult Population ◽

Reference Intervals ◽

Serum Copper ◽

Biological Variation ◽

Serum Samples ◽

Reference Change Value ◽

Index Of Individuality ◽

Copper Zinc ◽

Clinically Significant ◽

Analytical Variation

Abstract Objectives Calculation of biological variation (BV) components is very important in evaluating whether a test result is clinically significant. The aim of this study is to analyze BV components for copper, zinc and selenium in a cohort of healthy Turkish participants. Methods A total of 10 serum samples were collected from each of the 15 healthy individuals (nine female, six male), once a week, during 10 weeks. Copper, zinc and selenium levels were analyzed by atomic absorption spectrometer. BV parameters were calculated with the approach suggested by Fraser. Results Analytical variation (CVA), within-subject BV (CVI), between-subject BV (CVG) values were 8.4, 7.1 and 4.3 for copper; 4.2, 9.1 and 13.7 for zinc; 7.6, 2.5 and 6.9 for selenium, respectively. Reference change values (RCV) were 30.46, 27.56 and 22.16% for copper, zinc and selenium, respectively. The index of individuality (II) values were 1.65, 0.66 and 0.36 for copper, zinc and selenium, respectively. Conclusions According to the results of this study, traditional reference intervals can be used for copper but we do not recommend using it for zinc and selenium. We think that it would be more accurate to use RCV value for zinc and selenium in terms of following significant changes in recurrent results of a patient.

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Vitamin E-Coated Dialyzer Inhibits Oxidative Stress

Blood Purification ◽

10.1159/000478971 ◽

2017 ◽

Vol 44 (4) ◽

pp. 288-293 ◽

Cited By ~ 4

Author(s):

Shiho Yamadera ◽

Yuya Nakamura ◽

Masahiro Inagaki ◽

Isao Ohsawa ◽

Hiromichi Gotoh ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Synthetic Polymer ◽

Intracellular Reactive Oxygen Species ◽

Ros Production ◽

Oxygen Species ◽

In Vitro Methods ◽

Stress Effects ◽

Intracellular Ros

Aim: To examine the effects of vitamin E-coated dialyzer on oxidative stress in vitro. Methods: A dialyzer with a synthetic polymer membrane (APS-11SA) and vitamin E-coated dialyzer (VPS-11SA) were connected to a blood tubing line, and U937 cells were circulated in the device. The circulating fluid was collected at 1, 2, 5, 10, 25, and 50 cycles, which are estimated numbers of passes through the dialyzer. Intracellular reactive oxygen species (ROS) production, malondialdehyde (MDA), and Cu/Zn-superoxide dismutase (SOD) were quantified. Results: Intracellular ROS production was increased in the first cycle by APS-11SA and was decreased throughout the experiment by VPS-11SA. Intracellular ROS production in the VPS-11SA device was lower, and MDA levels were decreased. MDA levels were lower during VPS-11SA processing than during APS-11SA processing. Cu/Zn-SOD levels remained unchanged. Conclusion: Our results highlight anti-oxidative-stress effects of a vitamin E-coated dialyzer.

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