Microbial Diversity by Substrate Utilization Profiles of Lignite Mines Samples of Gujarat, India

Microbial diversity studies of lignite mines of Rajpardi, Amod, Tadkeshwar and Panandhro, Gujarat, India, were carried out by substrate utilization-based, community-structure analysis. In Biologâ EcoPlates out of 31 different substrates, 1 to 28 substrates were metabolized. On the basis of these, similarity index and diversity indices were studied. From an extreme mine site Gram-positive, Gram-negative, fungi and yeasts were isolated and identified. One of the yeasts isolated is for the first time reported from a lignite mine ecosystem Apart from this auto- and heterotrophic iron oxidizers; sulphur oxidizers and sulphate reducers were also isolated. So, lignite mine ecosystems, inspite of the extreme environment showed rich microbial diversity.

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Thysanoptera diversity associated with Mexican lemon (Citrus aurantifolia Christm.)

RECUBRIMIENTO COMESTIBLE CON BASE DE PIÑON MEXICANO (Jatropha curcas) SOBRE LA CALIDAD DE CHAYOTE MINIMAMENTE PROCESADO ◽

10.32854/agrop.vi.1761 ◽

2020 ◽

Vol 13 (9) ◽

Author(s):

Francisco Javier Avendaño-Gutiérrez ◽

Salvador Aguirre Paleo ◽

Alejandro Morales Hernández ◽

Venecia Quesadas-Béjar

Keyword(s):

Biosphere Reserve ◽

Simple Random Sampling ◽

Diversity Indices ◽

Diaphorina Citri ◽

Program Calculation ◽

Homogeneous Relation ◽

Candidatus Liberibacter ◽

Scirtothrips Citri ◽

Species Richness And Abundance ◽

First Time

Objective: To calculate the monthly relative abundance of Thysanoptera species, according to the Margalef, Simpson and Shannon-Wiener diversity indices. Design / Methodology / Approach: The work was carried out in three geographic units with conventional management, during January-December, 2019 in the Reserva de la Biosfera Zicuirán-Infiernillo (Biosphere Reserve), Michoacán, Mexico. In each unit, 10 trees were selected through simple random sampling. Thrips counts were performed on ten shoots per tree every 15 d, for a total of 7200 shoots in the three geographic units. Thysanoptera individuals were placed in entomological jars. The variables were: number of thrips collected per shoot in sampled tree and geographic unit (orchard). To estimate the specific richness and structure of species, the program "calculation of diversity indices DIVERS" was used. Results: In the three geographical units studied, the recorded presence of Thysanoptera accounted for 12 to 17 species. For Nueva Italia 12 recorded species, two were permanent (16.66%), five abundant (41.66%), one scarce (8.3%) and four rare (33.33%). In Zicuirán, three species were permanent (17.64%), six abundant (35.29%), two scarce (11.76%) and six rare (35.29%). In Los Hoyos, four species were permanent (26.66%), four abundant (26.66%) and seven rare (46.66%). The abundance of species was represented by the genus Frankliniella and the species Scolothrips sexmaculatus and Scirtothrips citri. The highest species richness and abundance was found from January to May. In October and November, the value of the calculated indices was zero, which shows less richness and abundance of individuals. The best species uniformity was recorded during January and December, which meant a more stable and homogeneous relation. Study limitations/Implications. Pest resurgence, presence of Candidatus Liberibacter spp. and its vector Diaphorina citri. Findings / Conclusions: in Nueva Italia, 12 species were taxonomically determined; in Los Hoyos 15, and in Zicuirán 17 species, which are reported for the first time in the state of Michoacán, Mexico. At the geographic unit "Los Hoyos" diversity was higher, uniform and stable.

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Microbiota identified from preserved Anopheles

Malaria Journal ◽

10.1186/s12936-021-03754-7 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Bianca E Silva ◽

Zvifadzo Matsena Zingoni ◽

Lizette L. Koekemoer ◽

Yael L. Dahan-Moss

Keyword(s):

Microbial Diversity ◽

Vector Competence ◽

Mosquito Species ◽

Anopheles Funestus ◽

Cost Effective ◽

Diversity Indices ◽

Malaria Vectors ◽

Microbial Composition ◽

Culture Independent ◽

Culture Dependent

Abstract Background Mosquito species from the Anopheles gambiae complex and the Anopheles funestus group are dominant African malaria vectors. Mosquito microbiota play vital roles in physiology and vector competence. Recent research has focused on investigating the mosquito microbiota, especially in wild populations. Wild mosquitoes are preserved and transported to a laboratory for analyses. Thus far, microbial characterization post-preservation has been investigated in only Aedes vexans and Culex pipiens. Investigating the efficacy of cost-effective preservatives has also been limited to AllProtect reagent, ethanol and nucleic acid preservation buffer. This study characterized the microbiota of African Anopheles vectors: Anopheles arabiensis (member of the An. gambiae complex) and An. funestus (member of the An. funestus group), preserved on silica desiccant and RNAlater® solution. Methods Microbial composition and diversity were characterized using culture-dependent (midgut dissections, culturomics, MALDI-TOF MS) and culture-independent techniques (abdominal dissections, DNA extraction, next-generation sequencing) from laboratory (colonized) and field-collected mosquitoes. Colonized mosquitoes were either fresh (non-preserved) or preserved for 4 and 12 weeks on silica or in RNAlater®. Microbiota were also characterized from field-collected An. arabiensis preserved on silica for 8, 12 and 16 weeks. Results Elizabethkingia anophelis and Serratia oryzae were common between both vector species, while Enterobacter cloacae and Staphylococcus epidermidis were specific to females and males, respectively. Microbial diversity was not influenced by sex, condition (fresh or preserved), preservative, or preservation time-period; however, the type of bacterial identification technique affected all microbial diversity indices. Conclusions This study broadly characterized the microbiota of An. arabiensis and An. funestus. Silica- and RNAlater®-preservation were appropriate when paired with culture-dependent and culture-independent techniques, respectively. These results broaden the selection of cost-effective methods available for handling vector samples for downstream microbial analyses.

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The new use of Sorghum bicolor-derived SSR markers to evaluate genetic diversity in 17 Australian Sorghum species

Plant Genetic Resources ◽

10.1079/pgr200454 ◽

2005 ◽

Vol 3 (1) ◽

pp. 19-28 ◽

Cited By ~ 12

Author(s):

Sally L. Dillon ◽

Peter K. Lawrence ◽

Robert J. Henry

Keyword(s):

Genetic Diversity ◽

Sorghum Bicolor ◽

Ssr Markers ◽

Diversity Indices ◽

Basic Knowledge ◽

Apparent Lack ◽

Breeding Programmes ◽

Diversity Studies ◽

Potential Use ◽

Simple Sequence

The Sorghum genus is extremely diverse both morphologically and geographically, however, relatively few of the 25 recognized species have been evaluated genetically. The apparent lack of basic knowledge pertaining to the levels of genetic diversity both within and between the 17 Australian wild species is a major obstacle to both their effective conservation and potential use in breeding programmes. Twelve Sorghum bicolor-derived simple sequence repeat (SSR) markers were evaluated for cross-species amplification in all 25 Sorghum species. The SSR markers were highly polymorphic, with diversity indices ranging from 0.59 to 0.99 with mean of 0.91. Five markers combined were able to differentiate 24 of the 25 Sorghum species, with intra-species polymorphism apparent. Sorghum bicolor-derived SSRs have proven to be an efficient source of markers for genetic diversity studies of the relatively poorly characterized Australian indigenous Sorghum species.

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Biodiversity in the Andean Mountains: Two new rain frogs of the genus Pristimantis (Anura: Craugastoridae) from the northern Cordillera Central in Colombia

Zootaxa ◽

10.11646/zootaxa.5040.3.2 ◽

2021 ◽

Vol 5040 (3) ◽

pp. 334-364

Author(s):

MAURICIO RIVERA-CORREA ◽

GUSTAVO A. GONZÁLEZ-DURÁN ◽

ANA M. SALDARRIAGA-GÓMEZ ◽

SEBASTIÁN DUARTE-MARÍN

Keyword(s):

New Species ◽

Species Delimitation ◽

Intraspecific Diversity ◽

Conservation Strategies ◽

Phylogenetic Position ◽

The Andes ◽

Two New Species ◽

Diversity Studies ◽

First Time ◽

Central Cordillera

Frequent biodiversity sampling and monitoring programs often lead to relevant taxonomic findings. Here, as a product of different field expeditions to two places in the northern Andes, we discover and describe two new species of rain frogs of the genus Pristimantis from the cloud forests of the Central Cordillera of Colombia. Pristimantis chocolatebari sp. nov. is diagnosed by having the following character states: dentigerous process of vomer triangular, snout large and protruding, dorsum smooth, vocal slits and vocal sac present, groin and hidden part of the thighs yellow. Pristimantis carylae sp. nov. is diagnosed by having iris copper red, dentigerous process of vomer oval, snout short and rounded, dorsum finely shagreen with scattered tubercles, double nuptial pad in males, groin and hidden part of thighs pink. We also infer, for the first time, the phylogenetic position of P. permixtus, P. platychilus, and two candidate new species. In addition, we emphasize not to use genetic distance as the only source of evidence for species delimitation, considering the high intraspecific diversity found in one of the species described here. Finally, we highlight the relevance of different habitat and ecosystem conservation strategies to promote amphibian diversity studies in the Andes.

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Protein-Coding Genes as Alternative Markers in Microbial Diversity Studies

Encyclopedia of Metagenomics ◽

10.1007/978-1-4899-7478-5_734 ◽

2015 ◽

pp. 596-601

Author(s):

Martin Wu

Keyword(s):

Microbial Diversity ◽

Protein Coding ◽

Protein Coding Genes ◽

Diversity Studies

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A Study of Zooplankton Community in Dukan Lake, Kurdistan Region-Iraq, with a New Record of Craspedacusta sowerbii Lankester (1880) Medusa (Cnidaria: Hydrozoa)

Baghdad Science Journal ◽

10.21123/bsj.14.4.735-741 ◽

2017 ◽

Vol 14 (4) ◽

pp. 735-741 ◽

Cited By ~ 1

Author(s):

Baghdad Science Journal

Keyword(s):

Similarity Index ◽

Chemical Properties ◽

Zooplankton Community ◽

Ion Concentration ◽

Hydrogen Ion Concentration ◽

Alkaline Side ◽

Physical And Chemical ◽

First Time ◽

Jaccard's Similarity Index ◽

And Chemical Properties

A study of Zooplankton community has been carried out at four selected sites on Dukan Lake. Samples of water and zooplankton were collected monthly for the period from July 2015 to February 2016. Some physical and chemical properties of water were studied and the results showed that the air temperature were ranged from 0 to 36.16 °C, water temperature ranged from 2.83 to 34.66 °C, hydrogen ion concentration of studied sites were found to lie in alkaline side, it was ranged between 6.87 to 8.57, electrical conductivity ranged from 190.79 to 850.08 µs.cm¹, turbidity ranged from 0.9-7.7 NTU, and dissolved oxygen from 3.3 to 6.8 mg.l-¹ while BOD5 were ranged from 0.53 to 34.66 mg.l-¹. Concerning to the zooplankton, 37 species were identified which belonged to Cladocera (48.38%), Copepod (43.28%), Rotifera (8.23%), Targigrada (0.08%) and Cnidaria (0.1%). The medusa of Craspedacusta sowerbii Lankester (1880) was recorded for the first time in Iraq. Regarding to zooplankton community, rotifer were ranged between 0 to 690.91 ind.m-3, Copepoda from 54.55 to 5927.27 ind.m-3 and Cladocera ranged from 18.18 to 6072.73 ind.m-3. According to Shanon-Weiner index, species diversity for zooplankton invertebrates was ranged from 0.325 to 1.091 bits/ind. Jaccard’s similarity index showed that the highest similarity was recorded between site (1) and site (4) with 40.74%.

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First fossil record of a nymph (Ephemeroptera, Teloganellidae) from the Indian subcontinent

Zootaxa ◽

10.11646/zootaxa.4838.1.8 ◽

2020 ◽

Vol 4838 (1) ◽

pp. 137-142

Author(s):

PRIYA AGNIHOTRI ◽

KAJAL CHANDRA ◽

ANUMEHA SHUKLA ◽

HUKAM SINGH ◽

RAKESH C. MEHROTRA

Keyword(s):

Fossil Record ◽

Indian Subcontinent ◽

Early Eocene ◽

Lignite Mine ◽

Modern Species ◽

The Family ◽

Mayfly Nymph ◽

Modern Genus ◽

First Time ◽

A New Species

A fossil of a mayfly nymph that shows similarities with the modern genus Teloganella Ulmer, 1939 of the family Teloganellidae is recorded for the first time from the Indian subcontinent. It is systematically described from the Gurha lignite mine of Bikaner, Rajasthan which belongs to the Palana Formation (late Paleocene-early Eocene). As assignment of the fossil to a modern species of Teloganella is difficult due to indistinguishable location of gills in the impression, a new species, Teloganella gurhaensis Agnihotri et al., sp. nov. is instituted to include this fossil naiad resembling the extant Teloganella.

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Bacterial and Fungal Diversity Inside the Medieval Building Constructed with Sandstone Plates and Lime Mortar as an Example of the Microbial Colonization of a Nutrient-Limited Extreme Environment (Wawel Royal Castle, Krakow, Poland)

Microorganisms ◽

10.3390/microorganisms7100416 ◽

2019 ◽

Vol 7 (10) ◽

pp. 416 ◽

Cited By ~ 2

Author(s):

Dyda ◽

Pyzik ◽

Wilkojc ◽

Kwiatkowska-Kopka ◽

Sklodowska

Keyword(s):

Microbial Community ◽

Microbial Diversity ◽

High Throughput Sequencing ◽

Extreme Environment ◽

Microbial Colonization ◽

Marker Genes ◽

Lime Mortar ◽

Preventive Actions ◽

Important Challenge ◽

Cultural Heritage Objects

Biodeterioration is a serious threat to cultural heritage objects and buildings. The deterioration of a given material often incurs irreparable losses in terms of uniqueness and historical value. Hence preventive actions should be taken. One important challenge is to identify microbes involved in the biodeterioration process. In this study, we analyzed the microbial diversity of an ancient architectonical structure of the Rotunda of Sts. Felix and Adauctus, which is a part of the Wawel Royal Castle located in Krakow, Poland. The Rotunda is unavailable to tourists and could be treated as an extreme habitat due to the low content of nutrients coming either from sandstone plates bound with lime mortar or air movement. Microbial diversity was analyzed with the use of the high-throughput sequencing of marker genes corresponding to fragments of 16S rDNA (for Bacteria) and ITS2 (internal transcribed spacer 2) (for Fungi). The results showed that the microbial community adhered to wall surfaces is, to a large extent, endemic. Furthermore, alongside many microorganisms that could be destructive to masonry and mortar (e. g., Pseudomonas, Aspergillus), there were also bacteria, such as species of genera Bacillus, Paenisporosarcina, and Amycolatopsis, that can positively affect wall surface properties by reducing the damage caused by the presence of other microorganisms. We also showed that airborne microorganisms probably have little impact on the biodeterioration process as their abundance in the microbial community adhered to the ancient walls was very low.

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I Macroeteroceri della Riserva Naturale Orientata “Laghetti di Marinello” (Sicilia nord-orientale) (Lepidoptera Heterocera)

Bollettino della Società Entomologica Italiana ◽

10.4081/bollettinosei.2012.31 ◽

2012 ◽

pp. 31-43

Author(s):

Marco Infusino ◽

Concetta Calabrò ◽

Salvatore Saitta ◽

Stefano Scalercio

Keyword(s):

Light Source ◽

Sono State ◽

Abundant Species ◽

Diversity Indices ◽

Climatic Conditions ◽

Number Of Species ◽

Sono Stati ◽

Natural Reserve ◽

North Eastern ◽

First Time

Riassunto - Gli scopi di questa ricerca sono quelli di contribuire alla conoscenza della macrolepidotterofauna della Sicilia nord-orientale e di caratterizzare la comunità presente nella Riserva Naturale Orientata “Laghetti di Marinello”. Sono stati effettuati 23 campionamenti tra dicembre 2007 e dicembre 2008 col metodo della caccia al lume. Sono stati catturati 2.537 individui appartenenti a 160 specie, tra cui Chloroclystis v-ata e Laelia coenosa, segnalate per la prima volta in Sicilia; importanti anche le segnalazioni di Calamodes subscudularia, Mythimna languida e M. joannisi, specie piuttosto rare e localizzate. Le specie più abbondanti sono state: Eilema caniola, Eublemma viridula, Idaea filicata, Dysauxes famula e Idaea seriata, che da sole rappresentano il 42% di tutta la comunità, favorite dalla presenza delle rispettive piante nutrici e dall’elevato adattamento alle condizioni climatiche del sito. Il numero di specie e i valori degli indici di diversità indicano un basso livello di biodiversità ospitata, ma la fenologia, le analisi corologica e autoecologica delle specie, insieme ai confronti effettuati con altre faune, indicano una comunità ben caratterizzata e peculiare, diretta espressione delle condizioni abiotiche e biotiche della Riserva.Abstract - Macroheterocera of the Oriented Natural Reserve “Laghetti di Marinello” (north-eastern Sicily) (Lepidoptera: Heterocera). The aim of this research is to contribute to the knowledge of the fauna of macrolepidoptera of north-eastern Sicily and to describe the community present in the Oriented Natural Reserve “Laghetti di Marinello”. 23 samples were carried out from December 2007 to December 2008 using a white vertical sheet reflecting a light source. 2.537 specimens belonging to 160 species were captured. Chloroclystis v-ata and Laelia coenosa were reported for the first time in Sicily, Calamodes subscudularia, Mythimna languida and M. joannisi, very rare and localized species, were also reported. The most abundant species were: Eilema caniola, Eublemma viridula, Idaea filicata, Dysauxes famula and Idaea seriata, which represent the 42% of the whole community and are favoured by the presence of their feeding plants and by their high adaptability to the climatic conditions of the place. The number of species and the values of the diversity indices show a low level of biodiversity, but phenology, chorological and autoecological analysis of the species, with comparisons made with other faunae, show a well characterized and peculiar community, direct expression of the abiotic and biotic conditions of the Reserve.

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