Influence of the Number of Pathogen Causing Banana Fusarium Wilt and Soil Factors on the Infection Degree of Banana Plants

2013 ◽  
Vol 781-784 ◽  
pp. 1989-1993 ◽  
Author(s):  
Xiao Deng ◽  
Qin Fen Li ◽  
Chun Yuan Wu ◽  
Jing Kun Liu
Keyword(s):  
Fusarium Wilt ◽  
Rhizosphere Soil ◽  
Soil Samples ◽  
Available P ◽  
Nutrient Concentrations ◽  
Soil Factors ◽  
Plate Method ◽  
Banana Production ◽  
Plant Infection ◽  
Dilution Plate Method

Banana fusarium wilt is one of the soil-borne diseases of seriously threat to banana production in the world. However, there is no effective method for prevention. The objective was providing theoretical basis for field diagnosis of banana fusarium wilt. Thirty rhizosphere and non-rhizosphere soil samples were collected from three typical banana plots (Jianfeng, Shiyuetian, Chongpo) infected by banana fusarium wilt. Applying dilution plate method to estimate the pathogenic number, fourteen soil physicochemical properties and nutrient concentrations were chemically determined. Results showed that pathogenic number and nutrient concentrations in rhizosphere soil were higher than non-rhizosphere soil, the pathogenic number and pH in rhizosphere soils significantly increased with increasing of plant infection grades, while the concentrations of available P and B decreased with increasing of plant infection grades. It was concluded that infection degree of banana plants was mainly determined by pathogenic number, pH and the concentrations of available P and B.

Metal accumulation by Arabidopsis halleri subsp. gemmifera at a limestone mining site

2015 ◽  
Vol 63 (2) ◽  
pp. 134 ◽  
Author(s):  
Aki Kosugi ◽  
Jun Tamaru ◽  
Kazumi Gotou ◽  
Hazuka Y. Furihata ◽  
Akifumi Shimizu ◽  
...  
Keyword(s):  
Calcareous Soil ◽  
Metal Accumulation ◽  
Rhizosphere Soil ◽  
Sampling Site ◽  
Calcareous Soils ◽  
Soil Samples ◽  
Arabidopsis Halleri ◽  
Soil Factors ◽  
Mining Site ◽  
Zn Concentration

Arabidopsis halleri subsp. gemmifera, a close wild relative of A. thaliana in eastern Asia, is an important model species of Brassicaceae used to study cadmium (Cd) and zinc (Zn) tolerance and hyperaccumulation in plants. To investigate the effects of soil factors on metal accumulation in this plant, we collected plants and rhizosphere soil samples from a limestone mining site on Mount Ibuki, and compared them with those collected from non-calcareous soil in Japan. Irrespective of the sampling site, all the plants efficiently accumulated Cd in shoot tissues. The plants growing on non-calcareous soil also accumulated Zn in shoot tissues, but shoot Zn concentration in plants growing on calcareous soils was below the level required for hyperaccumulators. The pH of calcareous soil samples was between 7.68 and 8.21. Total Zn contents were similar in calcareous and non-calcareous soils, but the amounts of Zn extractable by 0.1 M HCl were lower in calcareous than in non-calcareous soil. These results indicate that the properties of calcareous soil affect metal accumulation in plants.

Screening and antimicrobial activity of actinomycetes isolated from the rhizosphere of Clitoria ternatea

2016 ◽  
Vol 1 (01) ◽  
Author(s):  
Vemavarapu Bhaskara Rao ◽  
Kandlagunta Guru Prasad ◽  
Krishna Naragani ◽  
Vijayalakshmi Muvva
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Calcium Carbonate ◽  
Rhizosphere Soil ◽  
Soil Samples ◽  
Antimicrobial Spectrum ◽  
Clitoria Ternatea ◽  
Primary Screening

The air dried rhizosphere soil samples pretreated with calcium carbonate was employed for the isolation of actinomycete strains. Serial dilution plate technique was used for the isolation of actinomycetes. A total of 20 actinomycete strains designated as BS1-BS20 were isolated from the rhizosphere of medicinal plant Clitoria ternatea. All the 20 strains were subjected to primary screening for antimicrobial activity. Among the 20 strains screened, 10 strains exhibited high antimicrobial spectrum against Staphylococcus aureus, Escherichia coli and Candida albicans.

KEBERADAAN BAKTERI PELARUT FOSFAT DAN AKTIVITAS ENZIM FOSFATASE TANAH DAERAH PERAKARAN TANAMAN OBAT DARI KEBUN RAYA CIBODAS

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Suliasih Suliasih
Keyword(s):  
Alkaline Phosphatase ◽  
Soil Sample ◽  
Rhizosphere Soil ◽  
Botanical Garden ◽  
Soil Enzyme ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Sp ◽  
Phosphate Solubilizing ◽  
Serratia Rubidaea

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 – 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of “Lavender”.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase

Screening of Antagonistic Bacterium to Control Konjac Soft Rot

2013 ◽  
Vol 726-731 ◽  
pp. 4427-4430
Author(s):  
Guo Hua Chen
Keyword(s):  
Bacterial Strain ◽  
Bacterial Pathogen ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Field Trials ◽  
Antagonistic Effect ◽  
Soil Samples ◽  
Plate Method ◽  
Antagonistic Bacterium

Konjac soft rot is a bottleneck limiting konjac yield caused by bacterial strain of Erwinia carotovora var. carotovora. In order to control konjac soft rot, soil samples were collected, and each sample was spread on surface of a plate seeded with E. carotovora var. carotovora in advance. Strains expressing antagonistic activities were selected and then isolated with streak plate method. One bacterial strain (named Z10) was obtained from soil by this method. In field trials, strain Z10 still showed antagonistic effect against the bacterial pathogen.

scholarly journals Trace elements in aerial parts and rhizosphere of Thymus pannonicus All.

2011 ◽  
Vol 6 (4) ◽  
pp. 616-623 ◽  
Author(s):  
Jelena Arsenijević ◽  
Slavica Ražić ◽  
Zoran Maksimović ◽  
Svetlana Đogo
Keyword(s):  
Trace Elements ◽  
Soil Acidity ◽  
Rhizosphere Soil ◽  
Absorption Spectrometry ◽  
Soil Samples ◽  
Acid Digestion ◽  
Aerial Parts ◽  
Physiological Properties ◽  
Flameless Atomic Absorption Spectrometry ◽  
Zinc Levels

AbstractThis paper brings out the results of the study on the levels of selected trace elements (Cu, Fe, Mn, Zn and Cr) in aerial parts of Thymus pannonicus All. (Lamiaceae) and rhizosphere soil from twelve locations in Serbia. Prior to assays by flame and flameless atomic absorption spectrometry, samples were subjected to microwave-assisted acid digestion. Real and potential acidity of soil samples were also measured. Obtained results for soil samples, although slightly higher for some elements (Cu: 12.38–45.18 mg/kg; Fe: 22102–46193 mg/kg; Mn: 776.95–4901.27 mg/kg; Zn: 62.27–214.02 mg/kg; Cr: 48.86–69.13 mg/kg), were found to fit into biogeochemical background. Element contents in plant samples differed depending on collecting site (Cu: 5.26–14.07 mg/kg; Fe: 25.92–1454.07 mg/kg; Mn: 89.29–278.25 mg/kg; Zn: 1.81–10.64 mg/kg; Cr: 1.11–3.51 mg/kg), which can be partly explainable by different nutrient availability influenced by soil acidity. Zinc levels in T. pannonicus were below expected and seem to be strongly influenced by plant physiological properties.

scholarly journals Studies on the arbuscular mycorrhizal fungal association in some medicinal plant species of paithal hills, western ghats kannur district, kerala

2020 ◽  
Vol 7 (2) ◽  
pp. 30-38
Author(s):  
Santhoshkumar S ◽  
Nagarajan N ◽  
Sree Priya S
Keyword(s):  
Plant Species ◽  
Root Colonization ◽  
Rhizosphere Soil ◽  
Fungal Spores ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples ◽  
Fungal Colonization ◽  
The Family ◽  
Spore Population ◽  
Arbuscular Mycorrhizal Fungal

In the present study to analyzed that the arbuscular mycorrhizal fungal spores in root colonization and spore population in rhizosphere soils samples in various medicinal at Paithal hills,Western Ghats of Kannur district, Kerala, India. Root and rhizosphere soil samples were collected during the month of August, 2018-March, 2019 from the surface to 30 cm depth as well as pH were also recorded. Totally 30 plant species belonging to 19 families were collected and identified. The present result showed arbuscular mycorrhizal spore population in the rhizosphere soil and root colonization of all the plant species. A total of 19 AM fungal spores were recovered from the rhizosphere soil samples in this study region. The Glomus was dominant had seen in rhizosphere soil samples in all the medicinal plant species. The maximum spore population was found in the rhizosphere soil samples of Mimosa pudica (590/100g of soil) which belongs to the family Mimosaceae and the lowest spore population was observed in the Terminalia bellirica 135/100g of soil) belongs to Combretaceae family. The highest  78 % AM fungal colonization was found in roots of Euphorbia hirta belongs to the family Euphorbiaceae. While the lowest 11 % AM fungal colonization was found in the root of Sida acuta belongs to the family Malvaceae.

scholarly journals Effect of Soil Nutrient Management on P transformation under Protected Cultivation

2020 ◽  
Author(s):  
Khalid Hussain ◽  
Muniza Sarfraz
Keyword(s):  
Nutrient Management ◽  
Acid Soils ◽  
Essential Element ◽  
Soil Samples ◽  
Available P ◽  
Single Factor ◽  
P Fractions ◽  
North East ◽  
Factor Effect ◽  
Protected Cultivation

Background: Phosphorus, an essential element, plays a central role in cell metabolism and reproduction. It is a structural component of energy transferring molecules (ATP, ADP and AMP), nucleic acids, coenzymes, phosphor-proteins, phospholipids and sugar phosphates. In soils, the P does not occur abundantly as nitrogen or potassium. Despite its importance, only a proportion (~ 0.1-1.0 %) of it is available for use by plants and microorganisms because phosphorous has poor solubility and gets fixed in soil. Many studies had been done regarding nutrient management of soil in various parts of the world, but limited study was done in India and in North-East India, it is relatively unexplored. Thus, the main aim of this experiment was to find out how nutrient management influencing P fractions under protected cultivation. Methods: The investigation area from which soil samples were collected i.e., Horticultural Farm, Assam Agricultural University, Jorhat-13, Assam. The study was carried out under protected poly house condition in the year 2017-18. The test crop was Capsicum (Capsicum annum var. Swarna). The design of the experiment was Split-Split plot technique. Random soil samples were collected from different treatments under protected cultivation at a depth of 0-15 cm. The soil samples were collected at two different stages of the crop viz., flowering and fruiting stages at 45 and 115 days after planting respectively. Result: The present study revealed that single factor effect of nitrogen (N), phosphorus (P) and potassium (K) had a significant effect on different forms of P The readily available P forms tend to increase in the fruiting stage while the iron bound phosphates registered a decrease. This implies that iron bound phosphate form predominantly controlled available P in acid soils under protected cultivation. The study indicates that iron bound P may be considered as slowly available labile P for fertilizer scheduling under protected cultivation. The present study also revealed that single factor effect of nitrogen(N) and phosphorus(P) had a significant effect on yield of capsicum. Among the various P fractions, Pi-water and Pi-Fe contribute more towards yield of capsicum.

Adaptation of Lupinus angustifolius L. and L. pilosus Murr. to calcareous soils

1999 ◽  
Vol 50 (6) ◽  
pp. 1027 ◽  
Author(s):  
J. D. Brand ◽  
C. Tang ◽  
A. J. Rathjen
Keyword(s):  
Calcareous Soils ◽  
South Australia ◽  
Clay Content ◽  
Dry Weight ◽  
Lupinus Angustifolius ◽  
Nutrient Concentrations ◽  
Carbonate Content ◽  
Soil Factors ◽  
Shoot Dry Weight ◽  
Caco3 Content

Current varieties of narrow-leafed lupin (Lupin angustifolius L.) are poorly adapted to alkaline and calcareous soils found commonly throughout the south-estern Australian cropping zone. Apot experiment compared the growth of Lupinus angustifolius cv. Gungurru with L. pilosus P20954 in a range of soils collected throughout South Australia. The soils displayed a range of texture (clay, 3–82%), pH (1:5 soil:H2O, 7·0–9·6), and calcium carbonate content (CaCO3, 0–47%). Potting mix (pH 5·8) was used as the control. The plants were grown for 7 weeks with weekly measurements of chlorosis score and leaf number. At harvest, dry weights were recorded and the youngest fully expanded leaves were analysed for nutrient concentrations. The line P20954 grew much better in all the soils than Gungurru in terms of plant dry weight relative to the control soil, this being particularly evident in the calcareous soils. Chlorosis score correlated highly with shoot dry weight for Gungurru, but not for P20954. The main soil factor contributing to the chlorosis score of Gungurru was CaCO3 content, whereas none of the soil factors significantly affected P20954, although in Weeks 2 and 3 chlorosis score correlated with CaCO3 content. The dry weight of Gungurru was affected by a combination of factors including clay content, pH, and CaCO3 content, whereas the dry weight of P20954 was affected by most of the soil factors measured. The dry weight of P20954 was positively correlated with aluminium and magnesium concentrations. Concentrations of all nutrients were above critical levels for both genotypes grown in all soils. The results indicate that L. pilosus has the potential to be grown in areas where current varieties of L. angustifolius are poorly adapted.

Evaluation of Pseudomonas fluorescens Strains, Fungicides and Non-conventional Chemicals Against Botyrotinia ricini Causing Grey Mold Disease in Castor

2021 ◽  
Vol 12 (5) ◽  
pp. 339-347
Author(s):  
S. Ameer Basha ◽  
◽  
V. Ramya ◽  
A. Sajeli Begum ◽  
G. Raghavendra ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Rhizosphere Soil ◽  
Antagonistic Effect ◽  
Culture Technique ◽  
Soil Samples ◽  
Dual Culture ◽  
Integrated Disease Management ◽  
Grey Mold ◽  
Time Periods

A study was made to evaluate the efficacy of Pseudomonas fluorescens strains, fungicides and non-conventional chemicals against Botryotinia ricini, causing grey mold disease in castor, under in vitro conditions. Among the 40 strains isolated from rhizosphere soil samples of different crops across the State of Telangana, India, only eight strains inhibited the growth of B. ricini under dual culture technique, of which strains Pf 21 (90.56%), Pf 23 (88.89%), Pf 34 (86.11%) and Pf 36 (84.17%) were the most effective. Among the seven chemicals (four fungicides and three non-conventional chemicals) tested for their efficacy, carbendazim followed by propiconazole had significant antagonistic effect against B. ricini. Exposure of healthy castor capsules to B. ricini and P. fluorescens for different time periods revealed that strains Pf 34 and Pf 36 were effective in completely inhibiting the growth of B. ricini and hence these two strains have been identified as effective biocontrol agents, on par with carbendazim, which offer scope for sustainable and integrated disease management of grey mold disease in castor.

Isolation of Potential Alkaliphilic Cellulase-Producer Strains from Limestone Area of Perlis

2015 ◽  
Vol 754-755 ◽  
pp. 1054-1058
Author(s):  
Nor’izzah Zainuddin ◽  
Khadijah Hanim Abd Rahman ◽  
Abdul Razak Shaari ◽  
Siti Jamilah Hanim Mohd Yusof
Keyword(s):  
Selective Medium ◽  
Plate Method ◽  
Dilution Plate ◽  
Cellulolytic Fungi ◽  
The Future ◽  
Alkaline Cellulase ◽  
Dilution Plate Method ◽  
Soil Dilution ◽  
Positive Results ◽  
Producer Strains

Alkaliphilic cellulases-producer fungi were isolated from soil of limestone areas in Perlis. The potential strain was isolated by soil dilution plate method on enriched selective medium using CMC as substrate at different pH levels. Eleven isolates of different morphological colonies were screened using the hydrolysis capacity test by Gram’s iodine. Out of eleven colonies, five showed positive results as the hydrolysis zone formed. BK1 showed the highest hydrolysis capacity among all sample strains for every pH levels. Eventually, four strains were selected to be further explored as cellulolytic fungi for the production of alkaline cellulase in the future.

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