Study on Phenol-Degrading Characteristics of Hybrid Strains and Immobilized Cells

2014 ◽  
Vol 998-999 ◽  
pp. 330-335
Author(s):  
Cai Hong Yu ◽  
Wan Zhong Zhang ◽  
Chun Yan Li ◽  
Wen Juan Wang ◽  
Ying Huang

This study aims to enhance the bacteria’s ability to degrade phenol by exploring the phenol-degrading capability of hybrid strains and immobilized cells. Two phenol-degradation strains named as A1 and A2 were isolated respectively.The StrainA1 was identified as Burkholderia, while strain A2 was found to be Bacillus cereuswere. In addition, conditions including inoculum strains , pH value, temperature and dissolved oxygen were tested. . It was shown that: (1)Hybrid strains ( mixed A1 with A2) was better than single strain(A1 or A2) in degrading phenol because of the Synergistic effect. It can completely degraded phenol at the concentration of 300mg/ L, 500mg / L, 700mg / L within 12h, 24h, 48h respectively. (2) The optimum environmental conditions of phenol degradation for hybrid strains culture is 35°C, pH is 7.0, the inoculum strains is 10%. (3) It is beneficial to the degradation of phenol, when the speed of the shaker was improved. (4) Phenol-degradation rate and the tolerance of high concentration of phenol were significantly improved when the A1 and A2 were mixed immoblized.

2013 ◽  
Vol 859 ◽  
pp. 361-364 ◽  
Author(s):  
Jing Wang ◽  
Du Shu Huang ◽  
Wei Liu ◽  
Qing Shan Pan ◽  
Yong Min

Degradation properties of phenol using nano-TiO2 as photocatalyst in aqueous solution were investigated. The effect of annealing temperature and ionic modification on the degradation was studied. The results showed that, 500 °C annealed TiO2 was better than 700 °C annealed. Photocatalyst nano-TiO2 material doped with Fe3+ was prepared quickly by sol-gel process and was used as photocatalyst to degrade phenol solution of 100mg/L under UV irradiation for 3 hours. UV spectrophotometer testing was made and found that two peaks at 210 nm and 270 nm were significantly become low, indicating that the phenol has been degraded. The phenol degradation rate was 94.18%.


2014 ◽  
Vol 522-524 ◽  
pp. 665-671
Author(s):  
Zhen Huang ◽  
Yu Dong Song ◽  
Yue Xi Zhou ◽  
Xu Wen He ◽  
Jie Xu

Based on the problem of industrial wastewater of 4-Toluene Sulfonic Acid (4-TSA) with characteristics of high concentration and high toxicity, also low treatment efficiency by common biological treatment , this study was used aerobic biological fluidized bed (ABFB) to analyze the impact of 4-TSA treatment by changing water load chemical oxygen demand (COD), aeration amount, and pH value of input water, and so on technological parameters. The result showed that the average degradation rate of 4-TSA in wastewater can researched to 96.9% under condition of reactor steadily running, HRT=9 h, temperature 25°C, pH=8.0, ORP-141~-93 mV,DO 3.5~8.72 mg/L, the inflow water 4-TSA concentration 600 mg/L, aerobic amount 210 L/h. Therefore, the best aerobic amount to treat wastewater contained 4-TSA is 210 L/h in aerobic biological fluidized bed. The degradation rate of 4-TSA is decreased as the increasing of water load. The scanning electronic microscope (SEM) indicated that the bacillus is the mainly part on the biological carrier, suspended sludge is composed by aurous and bacillus.


2013 ◽  
Vol 859 ◽  
pp. 357-360 ◽  
Author(s):  
Xiao Dong Wang ◽  
Yang Lv ◽  
Meng Meng Li ◽  
Hong Ye Liu

This study investigates the degradation of Nonylphenol. The degradation affecting factors including solution ozone dosage, initial concentration, pH, temperature and ultraviolet. The results indicated that when the ozone contents changes, the Nonylphenols degradation rate changes as well. The higher ozone concentration contribute to the faster degradation; With the increase of initial concentration of Nonylphenol, the removal rate of it decrease on the contrary, while with the initial concentration increases, the quality of unit ozone degradation of Nonylphenol is ascenting and then tend to a constant; The remove rate of Nonylphenol is improving when the pH value vary from 4.86~10.34. The effect of Nonylphenols ozonation in higher temperatures is better than it is in lower temperature; Ultraviolet radiation is also favorable for the removal of Nonylphenol as it can shorten the reaction time as well as reduce the amount of ozone.


2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Jianghong Huang ◽  
Jianyi Xiong ◽  
Jianquan Liu ◽  
Weimin Zhu ◽  
Daping Wang

We prepared the poly-L-lactic acid (PLLA)/nanohydroxyapatite (n-HA) composite and investigated thein vitrodegradation of pure PLLA material and PLLA/n-HA composites in order to identify a suitable and ideal artificial bone tissue repair material. The water uptake, weight loss, and changes in the PBS pH value and in the mechanical properties of material were measured during the processes that PLLA and PLLA/n-HA biological composites were degraded in PBS. We also performed electron microscopic scanning of the material fracture surface and observed the microscopic morphologies of materials during the degradation process. We found that the degradation rate of the PLLA/n-HA material was slower than the PLLA material, and there was a little degradation of the PLLA/n-HA material at early stages. The PLLA/n-HA material also maintained the initial mechanical strength better than the pure PLLA material. The PLLA/n-HA material is thus a better material for artificial bone than the pure PLLA material.


2013 ◽  
Vol 864-867 ◽  
pp. 155-160 ◽  
Author(s):  
Xiao Dong Wang ◽  
Yang Lv ◽  
Hong Ye Liu ◽  
Meng Meng Li

This study investigates the degradation of bisphenol A (BPA). The degradation affecting factors including solution ozone dosage, initial concentration, pH, temperature and ultraviolet. The results indicated that when the ozone contents changes, the BPAs degradation rate changes as well. The higher ozone concentration contribute to the faster degradation; With the increase of initial concentration of BPA, the removal rate of it decrease on the contrary, while with the initial concentration increases, the quality of unit ozone degradation of BPA is ascenting and then tend to a constant; The remove rate of BPA is improving when the pH value vary from 4.86~10.34. The effect of BPAs ozonation in higher temperatures is better than it is in lower temperature; Ultraviolet radiation is also favorable for the removal of BPA as it can shorten the reaction time as well as reduce the amount of ozone.


2012 ◽  
Vol 433-440 ◽  
pp. 147-152
Author(s):  
Ying Chun Yang ◽  
Yuan Gang Lu ◽  
Zhi Xiang Ye ◽  
Li Ping He ◽  
Jing Yu

A catalyst based on Fe/active carbon (Fe/AC) and H2O2 as oxidant for the catalytic wet hydrogen peroxide oxidation of phenol in aqueous solution was investigated. The results indicate that the degradation rate of phenol(20mg/L) reach 90.5% in the presence of Fe/AC(2g/L) and hydrogen peroxide (0.5 %) at pH value 7 after 5 hours under normal temperature and atmospheric pressure. Kinetic studies of the degradation reaction show that the degradation rate of phenol nearly follows the first-order reaction. The reaction rate constant and activity energy are 0.4162 min-1 and 23.64 kJ/mol at 25°C, respectively.


2011 ◽  
Vol 391-392 ◽  
pp. 773-777 ◽  
Author(s):  
Ya Ling Huang ◽  
Ru Lin Fu ◽  
Zhen Kun Huang ◽  
Xian Su Cheng

A spherical amine modified lignin-base adsorbent had been prepared (L-BAA) by condensation polymerization of lignin with epoxy chloropropane and diamines. The modified products were characterized by FTIR spectra and scanning electron microscopy. Few researches on adsorbing Pb (II) of high concentration from aqueous had been reported. The spherical lignin-base adsorbent was used to adsorb Pb (II) of high concentration from aqueous solution. The effect of shaking time, pH value and temperature on adsorption had been investigated in the study. It was indicated that the adsorption was dependent on pH and temperature of Pb (II) aqueous solution. The maximum adsorption capacity was 151.0 mg/g at follow condition: pH value was 4.00 and temperature was 35°C. The adsorption capacity was better than other reported adsorbents.


2021 ◽  
Vol 9 (6) ◽  
pp. 1225
Author(s):  
Shanshan Zhao ◽  
Fengyuan Yang ◽  
Yuan Wang ◽  
Xiaomiao Fan ◽  
Changsong Feng ◽  
...  

The aim of this study was to gain deeper insights into the dynamics of fermentation parameters and the bacterial community during the ensiling of high-moisture alfalfa. A commercial lactic acid bacteria (YX) inoculant was used as an additive. After 15 and 30 days of ensiling, the control silage (CK) exhibited a high pH and a high concentration of ammoniacal nitrogen (NH3-N); Enterobacter and Hafnia-Obesumbacterium were the dominant genera. At 60 d, the pH value and the concentration of NH3-N in CK silage increased compared with 15 and 30 d, propionic acid and butyric acid (BA) were detected, and Garciella had the highest abundance in the bacterial community. Compared with CK silage, inoculation of YX significantly promoted lactic acid and acetic acid accumulation and reduced pH and BA formation, did not significantly reduce the concentration of NH3-N except at 60 d, and significantly promoted the abundance of Lactobacillus and decreased the abundance of Garciella and Anaerosporobacter, but did not significantly inhibit the growth of Enterobacter and Hafnia-Obesumbacterium. In conclusion, high-moisture alfalfa naturally ensiled is prone to rot. Adding YX can delay the process of silage spoilage by inhibiting the growth of undesirable microorganisms to a certain extent.


2021 ◽  
pp. 147737082199514
Author(s):  
Hila Avieli

There is growing interest in ageing offenders and their lives in prison. However, this subject is often studied from a deprivation perspective, focusing on issues such as lack of medical care and proper environmental conditions. This article highlights experiences of wellbeing while ageing in confinement, using the conceptual framework of successful ageing. An interpretive phenomenological analysis perspective was used to analyse the narratives of 18 older prisoners. The narratives revealed four themes: ‘Like all other older men’: comparing ageing in prison with ageing within the community; ‘Better than what I have outside’: prison as an escape from a life of loneliness, poverty and delinquency; ‘Here I get some respect’: the older prisoner as a mentor; and ‘I feel accomplished’: experiences of growth and self-discovery as a means for successful ageing in prison. The findings suggest that ageing in prison may not be perceived as a single, unified process, but as a personal and individual phenomenon, and that old age may facilitate positive changes in the lives of ageing offenders in prison.


2018 ◽  
Vol 53 ◽  
pp. 04016
Author(s):  
Juan Ma ◽  
Fang-yan Chen ◽  
Yu-bin Tang ◽  
Xin-gang Wang

Aiming at effectively controlling nonylphenol (NP) pollution, three bacterial strains were isolated from activated sludge and landfill leachate, which could grow with nonylphenol as sole carbon and energy source. The three nonylphenol-degrading bacteria isolated were named as WN6, SLY9 and SLY10, respectively. The morphological observation and 16S rDNA identification revealed that the strains belonged to Serratia sp., Klebsiella sp. and Pseudomonas putida, respectively. WN6 and SLY9 contained ALK gene, while WN6 and SLY10 harbored C12O genes. The three strains were combined together to form complex microorganisms ZJF. The ratio of Serratia sp. to Klebsiella sp. to Pseudomonas putida was 2:1:2 (volume ratio of bacterial suspension). Under the conditions that temperature was 30 ℃, pH was 6, inoculation amount was 10% (volume ratio), initial concentration of NP solutions was 20 mg/L, NP degradation rate by ZJF reached 73.82%, compared with any single strain of the three bacteria, NP degradation rate by ZJF increased more than 15% during 6 days. Bioremediation of nonylphenol-polluted the Yangtze River and the Ancient Canal water by ZJF ware simulated. After a 6-day incubation period, the degrading rate of nonylphenol in Ancient Canal water was close to 80%, and the degrading rate of nonylphenol in Yangtze River water was 72.84%.


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