Effect of Cisplatin/Curcumin-Loaded Polycaprolactone Nanoparticles on Oral Carcinoma Cells

2021 ◽  
Vol 901 ◽  
pp. 123-128
Author(s):  
Chaiyakarn Pornpitchanarong ◽  
Suwannee Panomsuk ◽  
Theerada Taesotikul

This work aimed to investigate the effect of cisplatin (CDDP)/curcumin (Cur)-loaded polycaprolactone nanoparticles (PCL-NPs) on an oral epidermal carcinoma cell line. PCL-NPs were fabricated using nanoprecipitation method employing poly(vinyl alcohol) and polysorbate 80 as stabilizers. Two anticancer compounds, CDDP and Cur, were incorporated into the PCL-NPs by entrapment technique. The physical characteristics of the NPs were evaluated. The presence of the drugs on the NPs was ascertained using Attenuated total reflection Fourier-transformed infrared (ATR-FTIR) spectroscopy, and the drug content was quantified by indirect method using ultraviolet spectroscopy and inductive coupled plasma-mass spectroscopy. The cytotoxic effect was demonstrated using MTT assay and the synergistic effect of both drugs was calculated by the combination index method using CompuSyn® software. The findings revealed that the PCL-NPs were less than 300 nm with narrow size distribution. The appropriate drug concentration for drug loading was 0.12 mg/mL of Cur and 0.02 mg/mL of CDDP, providing approximately 70% and 80% loading efficacy, respectively. The improved anticancer effect was observed in the cells treated with mixture of the drugs and the NPs loaded with dual drugs. Above all, CDDP/Cur-loaded PCL-NPs were successfully prepared. The delivery system exhibited good anticancer effect against oral cancer cells which may be attributed to the synergism effect of CDDP and Cur loaded on the NPs.

2021 ◽  
Author(s):  
Md. Sadek Bacchu ◽  
Md. Romzan Ali ◽  
Md. Ali Ahasan Setu ◽  
Selina Akter ◽  
Md. Zaved Hossain Khan

Abstract L-Cysteine coated zinc oxide (ZnO) nano hollow spheres were prepared as a potent drug delivery agent to eradicate Salmonella enterica serovar Typhimurium (S. typhimurium). The ZnO nano hollow spheres were synthesized by following the environmentally-friendly trisodium citrate assisted method and L-Cysteine (L-Cys) conjugate with its surface. ZnO/L-Cys@CFX nanocarrier drug has been fabricated by incorporating ceftizoxime with L-Cys coated ZnO nano hollow spheres and characterized using different techniques such as scanning electron microscope (SEM), attenuated total reflection Fourier transform infrared (ATR-FTIR), and X-ray diffraction (XRD) etc. Furthermore, the drug-loading and encapsulation efficiency at different pH levels was measured using UV-vis spectrometer and optimized. A control and gradual manner of pH-sensitive release profile was found after investigating the release profile of CFX from the carrier drug. The antibacterial activity of ZnO/L-Cys@CFX and CFX were evaluated through the agar disc diffusion method and the broth dilution method, which indicate the antibacterial properties of antibiotics enhance after conjugating. Surprisingly, the ZnO/L-Cys@CFX exhibits a minimum inhibitory concentration (MIC) of 5µg/ml against S. typhimurium is lower than CFX (20µg/ml) itself. These results indicate the nanocarrier can reduce the amount of CFX dosed to eradicate S. typhimurium.


2016 ◽  
Vol 26 (1) ◽  
pp. 28-37 ◽  
Author(s):  
Seo-Hee Kang ◽  
Yon-Suk Kim ◽  
Eun-Kyung Kim ◽  
Jin-Woo Hwang ◽  
Jae-Hyun Jeong ◽  
...  

2019 ◽  
Vol 107 (7) ◽  
pp. 2281-2287 ◽  
Author(s):  
Mai Tanaka ◽  
Toshinori Okinaga ◽  
Kenjiro Iwanaga ◽  
Kou Matsuo ◽  
Takashi Toyono ◽  
...  

2017 ◽  
Vol 390 (11) ◽  
pp. 1125-1134 ◽  
Author(s):  
Barbara Mayer ◽  
Svetlana Karakhanova ◽  
Nathalie Bauer ◽  
Li Liu ◽  
Yifan Zhu ◽  
...  

Pharmaceutics ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1170
Author(s):  
Caroline S. A. de Lima ◽  
Justine P. R. O. Varca ◽  
Kamila M. Nogueira ◽  
Gabriela N. Fazolin ◽  
Lucas F. de Freitas ◽  
...  

Papain is a therapeutic enzyme with restricted applications due to associated allergenic reactions. Papain nanoparticles have shown to be safe for biomedical use, although a method for proper drug loading and release remains to be developed. Thus, the objective of this work was to develop and assess the stability of papain nanoparticles in a prototype semi-solid formulation suitable for dermatological or topical administrations. Papain nanoparticles of 7.0 ± 0.1 nm were synthesized and loaded into carboxymethylcellulose- and poly(vinyl alcohol)-based gels. The formulations were then assayed for preliminary stability, enzyme activity, cytotoxicity studies, and characterized according to their microstructures and protein distribution. The formulations were suitable for papain nanoparticle loading and provided a stable environment for the nanoparticles. The enzyme distribution along the gel matrix was homogeneous for all the formulations, and the proteolytic activity was preserved after the gel preparation. Both gels presented a slow release of the papain nanoparticles for four days. Cell viability assays revealed no potential cytotoxicity, and the presence of the nanoparticles did not alter the microstructure of the gel. The developed systems presented a potential for biomedical applications, either as drug delivery systems for papain nanoparticles and/or its complexes.


Polymers ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 380 ◽  
Author(s):  
Yang Han ◽  
Na Liang ◽  
Pengfei Yan ◽  
Yoshiaki Kawashima ◽  
Fude Cui ◽  
...  

In this study, a redox-sensitive chitosan derivative with modifications by cholesterol, sulfhydryl, and mPEG (mPEG-CS(SH)-CHO) was successfully synthesized and characterized. Due to its amphiphilicity, the conjugate could spontaneously form micelles in an aqueous environment. The optimized paclitaxel (PTX)-loaded mPEG-CS(SH)-CHO micelles, with a mean diameter of 158 nm, zeta potential of +26.9 mV, drug loading of 11.7%, and entrapment efficiency of 88.3%, were successfully prepared. The results of an XRD study demonstrated that PTX was loaded in the core of the micelles in a non-crystalline state. Inspiringly, the PTX-loaded micelles possessed excellent anticancer effect but low toxicity to the body. It can be concluded that the mPEG-CS(SH)-CHO micellar system is a promising drug delivery carrier for the controlled release of PTX.


2011 ◽  
Vol 675-677 ◽  
pp. 827-830 ◽  
Author(s):  
Si Jun Zhu ◽  
Yuan Yuan Zhou ◽  
Ochi Takashi ◽  
Gang Wu

Polytetrafluoroethylene (PTFE) ultrafine fibers mats were prepared by electrospinning of an aqueous PTFE dispersion and a small amount of poly(vinyl alcohol) (PVA) and subsequent heat-treatment. The influence of dispersions composition on the morphology of fabricated PTFE ultrafine fiber mats was investigated. The fabricated mats were characterized by attenuated total reflection-Fourier transform infrared (ATR-FTIR) and koniology tester. The results show that the PVA/PTFE mass ratio of 1:5 was suitable for the PTFE fibers formation. All the PVA in the electrospun PVA/PTFE mats was removed at 350 °C~390°C. The fabricated PTFE ultrafine fiber mats have the potential to be used as high-temperature filter.


2016 ◽  
Vol 36 (4) ◽  
pp. 2059-2070 ◽  
Author(s):  
Qingqiang Shi ◽  
Xueping Shi ◽  
Gei Zuo ◽  
Wei Xiong ◽  
Haixing Li ◽  
...  

Oncotarget ◽  
2019 ◽  
Vol 10 (37) ◽  
pp. 3577-3578
Author(s):  
Yu-Chin Lin ◽  
Meng-Hsuan Wu ◽  
Tzu-Tang Wei ◽  
Yun-Chieh Lin ◽  
Wen-Chih Huang ◽  
...  

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