Antibacterial Activity Studies of Ni and SnO2 Loaded Chitosan Beads

In this work, Ni and SnO2loaded Chitosan nanomaterials are prepared by neutralization method, obtained by drop wise addition of Chitosan solution to a solution of NaOH, followed by cross-linking. The Structural, morphological and Raman analyses are carried out. The antibacterial activity is analysed against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa and evaluated by the calculation of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The obtained results show that, Ni-loaded nanoparticles could inhibit the growth of various bacteria better than SnO2. These results revealed that, the exposure of Staphylococcus aureus to the Chitosan nanoparticles led to the disruption of cell membranes and the leakage of cytoplasm.

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Phytochemical Evaluation and Antibacterial Activity of Fruit Extract of Solanum surattense Burm F. against Some Pathogenic Bacteria

International Journal of PharmTech Research ◽

10.20902/ijptr.2019.130109 ◽

2020 ◽

Vol 13 (1) ◽

pp. 79-87

Author(s):

R Dhanalakshmi ◽

J JaffarIqbal ◽

Nausath Jabeen K ◽

Noorul Zaithun Hidhaya M ◽

K Meena ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Salmonella Typhi ◽

Bacterial Strains ◽

Hydroalcoholic Extract ◽

Solanum Surattense

The objective of this study was to evaluate phytochemical constituents and antibacterial activity of hydroalcoholic extract of Solanum surattense fruit against some grampositive and gram-negative bacterial strains. The evaluation of antibacterial activity was carried out by using the disc diffusion method, determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Ciprofloxacin was used as positive control. Hydroalcoholic extract of Solanum surattense fruit containing alkaloids, flavonoids, phenol, saponins, terpenoids, glycosides, sterols, proteins and tannins. Efficacy data analysis showed that hydroalcoholic extracts of fruit of Solanum surattense (1 mg/ml) inhibited the growth of Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Salmonella typhi with mean diameters of inhibition zones being 24, 25, 28 and 30 mm respectively. On the other hand, minimum inhibitory concentration and minimum bactericidal concentration value of 0.062 and 0.25 mg/ml, 0.062 and 0.25 mg/ml, 0.312 and 0.125 mg/ml, 0.156 and 0.0312 mg/ml were recorded against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Salmonella typhi respectively. Hydroalcoholic extracts of Solanum surattense fruit have potent antibacterial activity against the different tested bacterial strains. This activity supports their use in treatment of infections caused by such resistant bacteria.

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Antibacterial Activity of Geraniol in Combination with Standard Antibiotics against Staphylococcus aureus, Escherichia coli and Helicobacter pylori

Natural Product Communications ◽

10.1177/1934578x1801300701 ◽

2018 ◽

Vol 13 (7) ◽

pp. 1934578X1801300 ◽

Cited By ~ 1

Author(s):

Subrat Kumar Bhattamisra ◽

Chew Hui Kuean ◽

Lee Boon Chieh ◽

Vivian Lee Yean Yan ◽

Chin Koh Lee ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Helicobacter Pylori ◽

Antibacterial Activity ◽

Synergistic Effect ◽

Inhibitory Concentration ◽

Therapeutic Potential ◽

E Coli ◽

Checkerboard Assay ◽

H Pylori

The antibacterial activity of geraniol and its effect in combination with ampicillin, amoxicillin and clarithromycin against Staphylococcus aureus, Escherichia coli and Helicobacter pylori was tested. The minimum inhibitory concentrations (MICs) and combinatory effects of geraniol against the bacteria were assessed by using the modified broth microdilution and checkerboard assay, respectively. The combinatory effect is expressed as fractional inhibitory concentration index (FICI). The MIC of geraniol against S. aureus, E. coli and H. pylori was found to be 11200, 5600, and 7325 μg/mL, respectively. A significant synergistic effect was observed with geraniol and ampicillin against S. aureus with FICI in the range 0.19 to 0.32. Geraniol and ampicillin exhibited a partial synergistic effect against E. coli. A similar effect was observed with geraniol and clarithromycin against S. aureus. A partial synergistic effect was observed with clarithromycin and geraniol against H. pylori with the FICI value in the range 0.86 to 0.89. An additive effect was observed with geraniol and amoxicillin combination against H. pylori. However, the amoxicillin and clarithromycin dose was reduced by thirty-two fold when combined with geraniol against H. pylori. The anti- H. pylori effect of geraniol with clarithromycin and amoxicillin could be of potential interest in the treatment of H. pylori infection and associated ulcers in humans. Further, geraniol, in combination with other antibiotics, has substantial therapeutic potential against S. aureus and E.coli infection.

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Preparation, Characterization and Antibacterial Activity of Chitosan Schiffbases

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.287-290.1947 ◽

2011 ◽

Vol 287-290 ◽

pp. 1947-1951 ◽

Cited By ~ 1

Author(s):

Jing Xian Xu ◽

Ye Ting Lin ◽

Qiang Lin ◽

Wen Yuan ◽

Xue Qiong Yin ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Schiff Bases ◽

Optical Density ◽

Elemental Analysis ◽

Antibacterial Activities ◽

Cp Mas Nmr ◽

And Staphylococcus Aureus ◽

Better Than

Two Schiff bases of chitosan (CTS) were synthesized from 4-methoxylbenzylaldehyde (CH3O-CTS) and 4-methylbenzylaldehyde(CH3-CTS). The Schiff bases were characterized by FTIR, DSC-TGA, solid13C CP-Mas NMR, and elemental analysis. Antibacterial activities of the Schiff bases against Escherichia coli and Staphylococcus aureus were measured by the optical density method. The antibacterial activity of the Schiff bases is better than that of the original CTS. The IC50of CH3O-CTS and CH3-CTS againstEscherichia coliis respectively 40.3 ppm and 43 ppm, which being 38.5 ppm and 39.5 ppm against Staphylococcus aureus, lower than IC50of chitosan, being 59.5 ppm and 52 ppm againstEscherichia coliand Staphylococcus aureus, respectively.

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Serum Inhibitory and Bactericidal Activity of Ciprofloxacin following Intravenous Administration

DICP ◽

10.1177/106002808902300603 ◽

1989 ◽

Vol 23 (6) ◽

pp. 456-460

Author(s):

Michael N. Dudley ◽

Hilary D. Mandler ◽

Kenneth H. Mayer ◽

Stephen H. Zinner

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Minimum Inhibitory Concentration ◽

Bactericidal Activity ◽

Resistant Strain ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

E Coli ◽

Dose Levels

Serum inhibitory and bactericidal titers were measured in nine healthy volunteers following single iv doses of ciprofloxacin 100, 150, and 200 mg. The median peak serum bactericidal titer (5 minutes following completion of a 30-minute infusion) against two highly susceptible strains of Escherichia coli ranged between 1:64 and 1:1024 and titers exceeded 1:8 for six hours for all dose levels. The bactericidal titers against two strains of Pseudomonas aeruginosa and a methicillin-resistant strain of Staphylococcus aureus were considerably lower, the median peak being 1:2 at all dose levels. Measured inhibitory and bactericidal titers at five minutes and one hour postinfusion were significantly greater than those predicted (measured serum ciprofloxacin concentration to minimum inhibitory concentration [MIC] or minimum bactericidal concentration [MBC]) for only one strain of E. coli. Intravenous doses of ciprofloxacin 100–200 mg produce high and sustained serum bactericidal titers against highly susceptible bacteria; considerably lower levels of activity are seen against bacteria having higher MICs and MBCs but still considered susceptible to the drug.

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Preparation of Cross-linked Chitosan Quaternary Ammonium Salt Hydrogel Films Loading Drug of Gentamicin Sulfate for Antibacterial Wound Dressing

Marine Drugs ◽

10.3390/md19090479 ◽

2021 ◽

Vol 19 (9) ◽

pp. 479

Author(s):

Jingjing Zhang ◽

Wenqiang Tan ◽

Qing Li ◽

Xiaorui Liu ◽

Zhanyong Guo

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ammonium Salt ◽

Quaternary Ammonium ◽

Quaternary Ammonium Salt ◽

Wound Dressing ◽

Cross Linking ◽

And Staphylococcus Aureus ◽

Hydrogel Films

Hydrogels, possessing high biocompatibility and adaptability to biological tissue, show great usability in medical applications. In this research, a series of novel cross-linked chitosan quaternary ammonium salt loading with gentamicin sulfate (CTMCSG) hydrogel films with different cross-linking degrees were successfully obtained by the reaction of chitosan quaternary ammonium salt (TMCS) and epichlorohydrin. Fourier transform infrared spectroscopy (FTIR), thermal analysis, and scanning electron microscope (SEM) were used to characterize the chemical structure and surface morphology of CTMCSG hydrogel films. The physicochemical property, gentamicin sulphate release behavior, cytotoxicity, and antibacterial activity of the CTMCSG against Escherichia coli and Staphylococcus aureus were determined. Experimental results demonstrated that CTMCSG hydrogel films exhibited good water stability, thermal stability, drug release capacity, as well as antibacterial property. The inhibition zone of CTMCSG hydrogel films against Escherichia coli and Staphylococcus aureus could be up to about 30 mm. Specifically, the increases in maximum decomposition temperature, mechanical property, water content, swelling degree, and a reduction in water vapor permeability of the hydrogel films were observed as the amount of the cross-linking agent increased. The results indicated that the CTMCSG-4 hydrogel film with an interesting physicochemical property, admirable antibacterial activity, and slight cytotoxicity showed the potential value as excellent antibacterial wound dressing.

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PROFIL KANDUNGAN KIMIA DAN AKTIVITAS ANTIBAKTERI EKSTRAK METANOL DAUN BAMBAN (Donax canniformis (G. Forst.) K. Schum.) TERHADAP Staphylococcus aureus

Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) ◽

10.22487/j24428744.2015.v1.i2.7899 ◽

2015 ◽

Vol 1 (2) ◽

pp. 141-148

Author(s):

Hidayatullah Hidayatullah ◽

Syariful Anam ◽

Muhamad Rinaldhi Tandah

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Phenolic Compounds ◽

Minimum Inhibitory Concentration ◽

Inhibitory Concentration ◽

Methanol Extract ◽

Minimum Bactericidal Concentration ◽

Dilution Method ◽

The Family

Bamban (Donax canniformis (G. Forst.) K. Schum.) is one of the family Marantaceae plant that has many uses such as traditional medicine. Methanol extract of bamban leaves contains phenolic, tannins and saponins compounds. The purpose of this research is to determine the class of compounds that has antibacterial activity against Staphylococcus aureus and determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methanol extract of bamban leaves. This extract was prepared using maceration method with methanol solvent. Determination the class of compounds was initiated by bioautografi test in order to determine spots which has have antibacterial activity. Subsequently, the spot were identified the class of compound using reagent spray FeCl3 and H2SO4 10%. The determination of MIC and MBC using dilution method. Research showed there are three compounds that had antibacterial activity. These compounds were predicted as spot I and spot II which were phenolic compounds and spot III as a saponin compound. MIC and MBC value of the methanol extract of leaves bamban leaves 8% and 13%, respectively.

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IN VITRO AND IN SILICO ANTIBACTERIAL ACTIVITY OF 1.5-BIS (3’-ETHOXY-4’-HYDROXYPHENYL)-1-4-PENTADIENE-3-ONE

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2018v10i5.25143 ◽

2018 ◽

Vol 10 (5) ◽

pp. 70

Author(s):

Agus Purwanggana ◽

Esti Mumpuni ◽

Esti Mulatsari

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Minimum Inhibitory Concentration ◽

Staphylococcus Epidermidis ◽

In Silico ◽

Inhibitory Concentration ◽

Inhibition Zone ◽

Antibacterial Study

Objective: The main objective of this research were screened in vitro and in silico of 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one as potential antibacterial agents.Methods: The in vitro antibacterial study was carried against Staphylococcus aureus, Staphylococcus epidermidis (gram positive) and Escherichia coli, Salmonella thypi (gram negative) using broth dilution method to determine Minimum Inhibitory Concentration (MIC), disc diffusion method to determine the diameter of inhibition zone. In silico antibacterial study was carried using computational software Protein-Ligand ANT System (PLANTS), computational docking was carried using receptor with Protein Data Bank (PDB) file 3MZD. The structures were optimized prior docking using YASARA, and MarvinSketch. The results of antibacterial testing were compared to two positive control drugs i. e amoxicillin and cefadroxil.Results: In vitro evaluation showed that 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has a better antibacterial activity than amoxicillin and cefadroxil with a Minimum Inhibitory Concentration (MIC) of 0.15 ppm and diameter of inhibition zone of 11.27±0.31, 11.35±0.39, 11.25±0.33, and 11.05±0.45 mm in Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Salmonella thypi, respectively. These results in line with in silico evaluation that showed 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has more negative docking score than amoxicillin, cefadroxil, and cloxacillin acyl as a native ligand on the 3MZD receptor.Conclusion: This results obtained in this research work were 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one compound potential as an antibacterial agent.

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Modulation of the Antibiotic Activity by Extracts fromAmburana cearensisA. C. Smith andAnadenanthera macrocarpa(Benth.) Brenan

BioMed Research International ◽

10.1155/2013/640682 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 25

Author(s):

Fernando G. Figueredo ◽

Emerson O. Ferreira ◽

Bruno F. F. Lucena ◽

Cícero M. G. Torres ◽

Daniel L. Lucetti ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Natural Products ◽

Inhibitory Concentration ◽

Antibiotic Activity ◽

Subinhibitory Concentration ◽

Antimicrobial Drugs ◽

Ethanol Extracts ◽

Multiresistant Strains

The aim of this study was to verify the possible interactions between ethanol extracts ofAmburana cearensisA. C. Smith andAnadenanthera macrocarpa(Benth.) Brenan, combined with six antimicrobial drugs against multiresistant strains ofStaphylococcus aureusandEscherichia coliisolated from humans. The antibacterial activity of the extracts was determined using the minimum inhibitory concentration (MIC). The microdilution assay was performed to verify the interactions between the natural products and the antibiotics using a subinhibitory concentration. The activity of amikacin associated with the extract ofAnadenanthera macrocarpaagainst EC 27 was enhanced, demonstrating an MIC reduction from 128 to 4 μg/mL. Among theβ-lactams, no potentiation on its activity was observed, with exception to the antagonism of the natural products with ampicillin againstS. aureus358.

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Antibacterial Activity of Murrayaquinone A and 6-Methoxy-3,7-dimethyl-2,3-dihydro-1H-carbazole-1,4(9H)-dione

International Journal of Microbiology ◽

10.1155/2014/540208 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 4

Author(s):

Biswanath Chakraborty ◽

Suchandra Chakraborty ◽

Chandan Saha

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Minimum Inhibitory Concentration ◽

Inhibitory Concentration ◽

Antibacterial Activities ◽

Gram Positive ◽

Gram Negative ◽

Naturally Occurring

The antibacterial activity of Murrayaquinone A (10), a naturally occurring carbazoloquinone alkaloid, and 6-methoxy-3,7-dimethyl-2,3-dihydro-1H-carbazole-1,4(9H)-dione (11), a synthetic carbazoloquinone, both obtained during the development of the synthesis of Carbazomycin G, having unique quinone moiety, was studied against Gram-positive (Bacillus subtilisandStaphylococcus aureus) and Gram-negative (Escherichia coliandPseudomonassp.) bacteria. Compound10showed antibacterial activities against both ofEscherichia coliandStaphylococcus aureuswhereas compound11indicated the activity againstStaphylococcus aureusonly. Both compounds10and11exhibited minimum inhibitory concentration (MIC) of 50 μg mL−1againstStaphylococcus aureus.

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Terpenoid components, antioxidant and antibacterial activities of essential oils from fresh immature and mature leaves of Blumea balsamifera, extracted with different hydrodistillation periods

10.1101/2021.02.01.429126 ◽

2021 ◽

Author(s):

Sirinapha Jirakitticharoen ◽

Wudtichai Wisuitiprot ◽

Pongphen Jitareerat ◽

Chalermchai Wongs-Aree

Keyword(s):

Escherichia Coli ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Essential Oils ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Antibacterial Activities ◽

Leaf Extracts ◽

Mature Leaves ◽

Antioxidant Capacities

AbstractVolatiles and antioxidant capacities in essential oils (EOs) from fresh immature and mature leaves of Blumea balsamifera, extracted with different extraction periods of hydrodistillation, were investigated. There were seven major terpenoid compounds in the leaf extracts, including 2 monoterpenes of camphor and L-borneol, and 5 sesquiterpenes of silphiperfol-5-ene, 7-epi-silphiperfol-5-ene, ß-caryophyllene, ɤ-eudesmol, and α-eudesmol. Different hydrodistillation periods resulted in different quantitates and compositions of the terpenoids in EOs. The yield of EOs from the immature leaves was 1.4 times higher than the mature ones, whereas 73% of the yield was collected from the first 6 h of hydrodistillation. Camphor and L-borneol were almost collected in the first 6 h, while ß-caryophyllene, silphiperfolene, and 7-epi-silphiperfolene were above 80%, but ɤ-eudesmol and α-eudesmol were only 32 and 54% released. ß-Caryophyllene, ɤ-eudesmol, and α-eudesmol were found higher in the mature leaf EOs. Antioxidant capacities in EOs were positively related to terpenoid contents. Antibacterial activity of EOs from the immature leaves was subsequently tested. Although EOs from the hydrodistillation period of 12-18 h contained fewer terpenoid compositions, it showed the same minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) on Escherichia coli and Pseudomonas aeruginosa, compared to 0-6 h EOs.

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