scholarly journals Modulation of the Antibiotic Activity by Extracts fromAmburana cearensisA. C. Smith andAnadenanthera macrocarpa(Benth.) Brenan

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Fernando G. Figueredo ◽  
Emerson O. Ferreira ◽  
Bruno F. F. Lucena ◽  
Cícero M. G. Torres ◽  
Daniel L. Lucetti ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Natural Products ◽  
Inhibitory Concentration ◽  
Antibiotic Activity ◽  
Subinhibitory Concentration ◽  
Antimicrobial Drugs ◽  
Ethanol Extracts ◽  
Multiresistant Strains

The aim of this study was to verify the possible interactions between ethanol extracts ofAmburana cearensisA. C. Smith andAnadenanthera macrocarpa(Benth.) Brenan, combined with six antimicrobial drugs against multiresistant strains ofStaphylococcus aureusandEscherichia coliisolated from humans. The antibacterial activity of the extracts was determined using the minimum inhibitory concentration (MIC). The microdilution assay was performed to verify the interactions between the natural products and the antibiotics using a subinhibitory concentration. The activity of amikacin associated with the extract ofAnadenanthera macrocarpaagainst EC 27 was enhanced, demonstrating an MIC reduction from 128 to 4 μg/mL. Among theβ-lactams, no potentiation on its activity was observed, with exception to the antagonism of the natural products with ampicillin againstS. aureus358.

Cholecalciferol, Ergosterol, and Cholesterol Enhance the Antibiotic Activity of Drugs

2018 ◽  
Vol 88 (5-6) ◽  
pp. 244-250 ◽  
Author(s):  
Jacqueline C. Andrade ◽  
Maria Flaviana B. Morais Braga ◽  
Gláucia Morgana M. Guedes ◽  
Saulo R. Tintino ◽  
Maria A. Freitas ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Cell Membrane ◽  
Antibiotic Activity ◽  
Subinhibitory Concentration ◽  
Bacterial Strains ◽  
Reported Study ◽  
Lipid Compounds ◽  
Multiresistant Strains

Abstract. Background: This is the first report demonstrating the antibiotic-modifying activity of cholecalciferol. Aim: In this study, cholecalciferol was evaluated against multiresistant strains of Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. Methods: The antibacterial and modulatory effects of cholecalciferol, ergosterol, and cholesterol (8–512 μg/mL) were evaluated by microdilution assay against multiresistant bacterial strains. Results: Cholecalciferol, when combined with aminoglycosides, was more effective against P. aeruginosa, reducing the concentration of amikacin and gentamicin necessary to inhibit bacterial growth from 156.25 to 39.06 μg/mL and from 39.06 to 9.76 μg/mL, respectively. It is possible that cholecalciferol, due to its lipid-soluble nature, had a lipophilic interaction with the cell membrane, enhancing antibiotic uptake. Cholesterol and ergosterol were used to see if the mechanism of action of cholecalciferol was similar to that of these lipid compounds. Ergosterol and cholesterol increased aminoglycoside activity, where the effect was greater with higher subinhibitory concentration of sterol. Conclusions: There is no reported study on the use of cholesterol and ergosterol as modulators of antibiotics or any other drug, making this the first study in this area highlighting the interaction between cholesterol, ergosterol, and cholecalciferol with regard to modifying aminoglycoside activity.

scholarly journals Antibacterial Activity of Geraniol in Combination with Standard Antibiotics against Staphylococcus aureus, Escherichia coli and Helicobacter pylori

2018 ◽  
Vol 13 (7) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Subrat Kumar Bhattamisra ◽  
Chew Hui Kuean ◽  
Lee Boon Chieh ◽  
Vivian Lee Yean Yan ◽  
Chin Koh Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Helicobacter Pylori ◽  
Antibacterial Activity ◽  
Synergistic Effect ◽  
Inhibitory Concentration ◽  
Therapeutic Potential ◽  
E Coli ◽  
Checkerboard Assay ◽  
H Pylori

The antibacterial activity of geraniol and its effect in combination with ampicillin, amoxicillin and clarithromycin against Staphylococcus aureus, Escherichia coli and Helicobacter pylori was tested. The minimum inhibitory concentrations (MICs) and combinatory effects of geraniol against the bacteria were assessed by using the modified broth microdilution and checkerboard assay, respectively. The combinatory effect is expressed as fractional inhibitory concentration index (FICI). The MIC of geraniol against S. aureus, E. coli and H. pylori was found to be 11200, 5600, and 7325 μg/mL, respectively. A significant synergistic effect was observed with geraniol and ampicillin against S. aureus with FICI in the range 0.19 to 0.32. Geraniol and ampicillin exhibited a partial synergistic effect against E. coli. A similar effect was observed with geraniol and clarithromycin against S. aureus. A partial synergistic effect was observed with clarithromycin and geraniol against H. pylori with the FICI value in the range 0.86 to 0.89. An additive effect was observed with geraniol and amoxicillin combination against H. pylori. However, the amoxicillin and clarithromycin dose was reduced by thirty-two fold when combined with geraniol against H. pylori. The anti- H. pylori effect of geraniol with clarithromycin and amoxicillin could be of potential interest in the treatment of H. pylori infection and associated ulcers in humans. Further, geraniol, in combination with other antibiotics, has substantial therapeutic potential against S. aureus and E.coli infection.

scholarly journals Eugenia uniflora L.: potential uses as a bioactive plant

2018 ◽  
Vol 85 (0) ◽  
Author(s):  
Gabriela Silva Moura ◽  
Ivan Júnior de Oliveira ◽  
Lisandro Tomas da Silva Bonome ◽  
Gilmar Franzener
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Natural Products ◽  
Total Phenolics ◽  
Antioxidant Compounds ◽  
Review Of The Literature ◽  
Bioactive Substances ◽  
Eugenia Uniflora

ABSTRACT: Natural products extracted from plants have always played an important role in the discovery of bioactive substances. This work carried out a review of the literature on the bioactive activities of Eugenia uniflora L. (Surinam cherries), as a potential plant in the various uses, be it medicinal, antimicrobial, antioxidant, insecticide and protective. In the literature, studies were found to confirm the antibacterial activity of E. uniflora leaves extract on Streptococcus, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The presence of antioxidant compounds as total phenolics, such as anthocyanins and flavonoids, induction in the protection of plants by phytoalexins, as well as insecticidal and anthelmintic effects by the use of the extract of the E. uniflora tree. Although these different potential biotics of E. uniflora have already been reported, further studies are still needed on the use of natural products extracted from E. uniflora for employment for different purposes.

scholarly journals Aktivitas Antibakteri Sabun Cuci Tangan yang Mengandung Ekstrak Metanol Rumput Laut Eucheuma spinosum (Antibacterial Activity Test of Eucheuma spinosum Methanol Extract Hand Wash)

MEDULA ◽  
2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Nur Illiyyin Akib ◽  
Mariani Triwatami ◽  
Andi Eka Purnama Putri
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Antibacterial Activities ◽  
Dilution Method ◽  
E Coli ◽  
Hand Wash ◽  
Ethanol Extracts ◽  
Physical And Chemical

Background: Eucheuma spinosum seaweed contain flavonoid, triterpenoid, alkaloids and polyphenol which has been widely used in antibacterial activity. Purpose(s):The aim of the research are to determine antibacterial activities of metanol extracts of E. spinosum against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922; to formulate hand wash of seaweed of methanol extract E. spinosum that has antibacterial activity and has physical and chemical stability. Methods: Seaweed E.spinosum methanol extract was derived by maceration method. Antibacterial activities of the extract were tested by liquid dilution and solid dilution method. Hand wash was formulated by mechanical dissolved methods. Antibacterial activity of hand wash were tested by liquid dilution and solid dilution method. Physical and chemical stabilities were conducted by cycling test. Results:. These were showed through minimum inhibitory concentration (MIC) of ethanol extracts of E. spinosum against S. aureus ATCC 25923 at concentrations of 6% and E. coli ATCC 25922 at concentrations of 6%. The minimum bactericidal concentration (MBC) of ethanol extracts of E. spinosum against S. aureus ATCC 25923 at concentrations of 8% and E. coli ATCC 25922 at concentrations of 8%. Formulation of hand wash seaweed E. spinosum methanol extract at concentrations of 8% and 10%. The test of stabilities results of hand wash changes were organoleptic, viscosity, pH, and foaming ability were accordance to qualified standard. The antibacterial activity of hand wash contains seaweed E. spinosum methanol extract at concentration of  8% and 10% have bactericidal activity againts S. aureus ATCC 25923 and E. coli ATCC 25922. Conclusion: Overall, these results suggested that formula of hand wash contains E. spinosum metanol extracts have antibacterial properties against S. aureus ATCC 25923 and E. coli ATCC 25922.Keywords: antibacterial, Eucheuma spinosum, hand wash, physical and chemical stability Latar Belakang: Rumput laut Eucheuma spinosum mengandung flavonoid, triterpenoid, alkaloid, dan polifenol yang memiliki aktivitas antibakteri. Tujuan: Penelitian ini bertujuan mengetahui aktivitas antibakteri ekstrak metanol E. spinosum terhadap Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922; membuat sediaan sabun cuci tangan dari ekstrak metanol E. spinosum yang memiliki aktivitas antibakteri dan stabil secara fisika dan kimia. Metode: Ekstrak metanol E. spinosum diperoleh dengan metode maserasi. Uji aktivitas antibakteri ekstrak dilakukan dengan metode dilusi cair dan dilusi padat. Sabun cuci tangan diformulasi dengan metode pencampuran mekanik. Uji aktivitas antibakteri sabun cuci tangan dilakukan dengan metode dilusi cair dan dilusi padat. Uji stabilitas fisika kimia dilakukan dengan metodecycling test. Hasil: Konsentrsi Hambat Minimum (KHM) ekstrak metanol E. spinosum terhadap S. aureus ATCC 25923 adalah 6% dan terhadap E. coli ATCC 25922 adalah 8%. Ekstrak metanol E. spinosum dapat diformulasi menjadi sabun cuci tangan dengan konsentrasi 8% dan 10%. Uji stabilitas menunjukkan bahwa perubahan organoleptik, viskositas, pH, dan kemampuan membentuk busa masih berada dalam nilai yang dipersyaratkan. Uji aktivitas antibakteri sabun cuci tangan yang mengandung ekstrak metanol E. spinosum dengan konsentrasi 8% dan 10% memiliki aktivitas antibakteri terhadap ATCC 25923 and E. coli ATCC 25922. Simpulan: Formula sabu cuci tangan yang mengandung ekstrak metanol E. spinosum memiliki aktivitas antibakteri terhadap ATCC 25923 and E. coli ATCC 25922.Kata kunci: antibakteri, Eucheuma spinosum, sabun cuci tangan, stabilitas fisika kimia

scholarly journals Evaluation of the antibacterial and modulatory activities of ethanolic excract of Calotropis procera (Aiton) W.T. Aiton against multiresistant bacterial strains

2021 ◽  
pp. 205-209
Author(s):  
Yuri Geraldo ◽  
Livia Leandro ◽  
Ana Raquel Silva ◽  
Fábia Campina ◽  
Ana Carolina Araújo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Calotropis Procera ◽  
Subinhibitory Concentration ◽  
Bacterial Strains ◽  
Resistant Species ◽  
Microdilution Method

The objective of this study was to evaluate the antibacterial and modulatory activities of ethanolic extract of Calotropis procera (Aiton) W.T. Aiton against resistant species. By microdilution method, the minimum inhibitory concentration (MIC) of the extract and modulation of the subinhibitory concentration MIC/8 to norfloxacine, gentamicin and imipenem against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. There was obtained 512 μg/mL to Pseudomonas aeruginosa. To Staphylococcus aureus, modulation showed synergism to norfloxacin and gentamicin, with imipenem against Pseudomonas aeruginosa and gentamicin against Escherichia coli. Based on these results, more studies are needed to test the antibacterial activity of the extract. El objetivo de este estudio fue evaluar la actividad antibacteriana y moduladora del extracto etanólico de Calotropis procera (Aiton) W.T. Aiton contra cepas multirresistentes de bacterias. Por el método de microdilución, fueron definidas la concentración inhibidora mínima (MIC) del extracto y la modulación con la concentración inhibidora CIM / 8 del extracto con norfloxacina, gentamicina e imipenem contra Staphylococcus aureus, Escherichia coli y Pseudomonas aeruginosa. Se obtuvo 512 μg/mL para Pseudomonas aeruginosa. Se descubrió sinergismo en el caso de Staphylococcus aureus, en la modulación con norfloxacina y gentamicina, mientras que con imipenem frente a Pseudomonas aeruginosa y con gentamicina para Escherichia coli. Con base en estos resultados, se necesitan más estudios para probar la actividad antibacteriana del extracto.

scholarly journals SUBSTANSI ANTIBAKTERI JAMUR ENDOFIT PADA LAMUN ASAL PERAIRAN TONGKAINA

2017 ◽  
Vol 5 (3) ◽  
pp. 83
Author(s):  
Galih Prasetyo ◽  
Remmy Mangindaan ◽  
Robert Antonius Bara
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Fungal Endophytes ◽  
Antibiotic Activity ◽  
Thalassia Hemprichii ◽  
Enhalus Acoroides ◽  
Rice Medium ◽  
Partition Process

Seagrass is a part of Phanerogamae, commonly has a symbiotic relationship with microbial endophytes. The types of microbes those have ability to produce bioactive compounds with potentially exploited for medical, agriculture and industrial purposes. Antibacterial testing using the modified Kirby-Bauer method. The fungi show a strong antibiotic activity which cultivated statically and Staphylococcus aureus was induced in rice medium for 10 days. The purpose of inducing bacteria to the culture is to stimulate a strong antibiotic activity through Silence Biosynthesis Pathway. Fungal isolates were macerated with 96% of ethanol for 24 hours. Partition process was performed by adding solvents (ethyl acetate, n-hexane, ethanol and water) to get n-hexane, ethanol and water fractions. All fractions were tested their activity against clinical isolates bacteria S. aureus and Escherichia coli. Ten fungal endophytes were isolated from seagrass Thalassia hemprichii and Enhalus acoroides. Two isolates derived from the leaf of both seagrass specimens (E.D.1 and Th.D.1) showed strong antibacterial activity against S. aureus only. Antibacterial activity test of each fraction both active isolates show in water and ethanol fractions. This indicates the active antibacterial compounds of both endophytic fungi have semi-polar and polar characteristics. However, bacterial induction has no effect on their antibacterial activity.Keywords : Endophytic fungi, Thalassia hemprichii, Enhalus acoroides, antibacterial activity Staphylococcus aureus and Escherichia coli.Lamun merupakan tanaman tingkat tinggi yang mempunyai hubungan simbiosis dengan mikroba jamur endofit. Mikroba endofit ini mempunyai kemampuan untuk memproduksi senyawa-senyawa bioaktif dengan potensi yang besar untuk dieksploitasi dan menghasilkan yang bermanfaat di bidang medis, pertanian, dan industri. Isolasi jamur dilakukan mengacu pada penelitian Bara et al (2013). Pengujian antibakteri dilakukan berdasarkan metode Kirby-Bauer yang dimodifikasi. Jamur memperlihatkan aktivitas antibiotik yang kuat dikultivasi statis dan di induksikan bakteri S. aureus dalam media nasi selama 10 hari. Tujuan pemberian bakteri pada kultur yaitu untuk memicu adanya aktivitas antibiotik yang lebih kuat melalui jalur biosintesis senyap (Silence Biosintethic Pathway) pada jamur tersebut. Isolat jamur di maserasi dengan menambahkan etanol 96% selama 24 jam. Proses partisi dengan menambahkan pelarut (etil asetat, n-heksan, etanol dan air) untuk memperoleh fraksi n-heksan, etanol dan air. Tiap fraksi diuji kembali aktivitas antibiotiknya pada bakteri S. aureus dan Escherichia coli. Hasil penelitian ini diperoleh sepuluh isolat jamur dari lamun T. hemprichii dan E. acoroides. Dua isolat daun (E.D.1 dan Th.D.1) menunjukkan aktivitas yang kuat terhadap bakteri S. aureus. Pengujian aktivitas antibakteri tiap fraksi kedua isolat jamur memperlihatkan fraksi air dan etanol yang menunjukkan aktivitas penghambat. Hal ini menunjukkan bahwa senyawa aktif antibakteri kedua jamur endofit berisfat semi polar dan polar. Induksi bakteri tidak memberikan pengaruh terhadap aktivitas antibakteri.Kata Kunci : jamur endofit, Thalassia hemprichii, Enhalus acoroides, aktivitas antibakteri Staphylococcus aureus dan Escherichia coli.

scholarly journals PENGARUH PEMANASAN, TINGKAT KEASAMAN (pH) DAN KONSENTRASI GARAM TERHADAP STABILITAS ANTI BAKTERI EKSTRAK ETANOL KULIT KAYU AKWAY (Drimys piperita Hook f.)

2019 ◽  
Vol 12 (2) ◽  
Author(s):  
Gino Nemesio Cepeda ◽  
Meike Meilan Lisangan ◽  
Isak Silamba ◽  
Nitia Nilawati ◽  
Eka Syartika
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Inhibitory Concentration ◽  
Heating Time ◽  
Diffusion Method ◽  
Ethanol Extracts ◽  
And Staphylococcus Aureus ◽  
Agar Well Diffusion Method

ABSTRACT  Akway (Drimys piperita) is a woody, evergreen and aromatic plant that belongs to family winteraceae. This plant is used by Sougb tribe lived in Sururey village, District of Anggi, to enhance the vitality of body. The objectives of the research were to determine antimicrobial stability of akway bark extracts influenced by heating time of 100OC, levels of acidity (pH) and salt contents.. Antimicrobial assays were done by using agar well diffusion method against four species of bacteria, i.e.  Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923. The results showed that ethanolic extracts of akway bark only inhibited growth of B. cereus and S. aureus with minimum inhibitory concentration 0,99% and 0,89% . The levels of concentration and acidity of ethanol extracts  influenced the antimicrobial capacity of extracts.. Whereas heating time on 100OC during 25 minutes and salt contents  up to 5% of extract solution did not influence the antimicrobial stability of  akway bark extracts. Key words : akway, extracts, antimicrobe, pH, Heating, salt ABSTRAK Akway (Drimys piperita) adalah tumbuhan berkayu, aromatik dan hijau sepanjang tahun dan tergolong dalam suku winteraceae. Tumbuhan ini digunakan oleh Suku Sougb yang bermukim di desa Sururey Distrik Anggi, untuk mengobati malaria dan meningkatkan vitalitas tubuh. Tujuan dari penelitian ini adalah untuk menentukan stabilitas antimikroba ekstrak kulit kayu akway pada waktu pemanasan ekstrak pada 100OC, tingkat keasaman (pH) dan kandungan garam. Pengujian antimikroba dilakukan dengan menggunakan metode difusi agar terhadap empat spesies bakteri yaitu Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923. Hasil menunjukkan bahwa ekstrak etanol kulit kayu akway hanya dapat menghambat bakteri Bacillus cereus dan Staphylococcus aureus dengan konsentrasi hambat minimum masing-masing adalah 0,99% dan 0,89%. Tingkat konsentrasi dan keasaman (pH) mempengaruhi kapasitas antimikroba ekstrak etanol kulit kayu akway. Sedangkan perlakuan pemanasan pada suhu 100OC dengan lama pemanasan sampai dengan 25 menit dan penambahan garam NaCl sampai konsentrasi 5%  tidak berpengaruh pada stabilitas antimikroba ekstrak etanol kulit kayu akway.  

scholarly journals IN VITRO AND IN SILICO ANTIBACTERIAL ACTIVITY OF 1.5-BIS (3’-ETHOXY-4’-HYDROXYPHENYL)-1-4-PENTADIENE-3-ONE

2018 ◽  
Vol 10 (5) ◽  
pp. 70
Author(s):  
Agus Purwanggana ◽  
Esti Mumpuni ◽  
Esti Mulatsari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Staphylococcus Epidermidis ◽  
In Silico ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Antibacterial Study

Objective: The main objective of this research were screened in vitro and in silico of 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one as potential antibacterial agents.Methods: The in vitro antibacterial study was carried against Staphylococcus aureus, Staphylococcus epidermidis (gram positive) and Escherichia coli, Salmonella thypi (gram negative) using broth dilution method to determine Minimum Inhibitory Concentration (MIC), disc diffusion method to determine the diameter of inhibition zone. In silico antibacterial study was carried using computational software Protein-Ligand ANT System (PLANTS), computational docking was carried using receptor with Protein Data Bank (PDB) file 3MZD. The structures were optimized prior docking using YASARA, and MarvinSketch. The results of antibacterial testing were compared to two positive control drugs i. e amoxicillin and cefadroxil.Results: In vitro evaluation showed that 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has a better antibacterial activity than amoxicillin and cefadroxil with a Minimum Inhibitory Concentration (MIC) of 0.15 ppm and diameter of inhibition zone of 11.27±0.31, 11.35±0.39, 11.25±0.33, and 11.05±0.45 mm in Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Salmonella thypi, respectively. These results in line with in silico evaluation that showed 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has more negative docking score than amoxicillin, cefadroxil, and cloxacillin acyl as a native ligand on the 3MZD receptor.Conclusion: This results obtained in this research work were 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one compound potential as an antibacterial agent. 

Antibacterial Activity Studies of Ni and SnO2 Loaded Chitosan Beads

2015 ◽  
Vol 832 ◽  
pp. 110-122 ◽  
Author(s):  
A. Ayeshamariam ◽  
G.V. Sankaracharyulu ◽  
M. Kashif ◽  
Shahid Hussain ◽  
Mohamed Bououdina ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Chitosan Nanoparticles ◽  
Chitosan Solution ◽  
Cross Linking ◽  
Chitosan Beads ◽  
Better Than

In this work, Ni and SnO2loaded Chitosan nanomaterials are prepared by neutralization method, obtained by drop wise addition of Chitosan solution to a solution of NaOH, followed by cross-linking. The Structural, morphological and Raman analyses are carried out. The antibacterial activity is analysed against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa and evaluated by the calculation of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The obtained results show that, Ni-loaded nanoparticles could inhibit the growth of various bacteria better than SnO2. These results revealed that, the exposure of Staphylococcus aureus to the Chitosan nanoparticles led to the disruption of cell membranes and the leakage of cytoplasm.

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