NIGERIAN PARA RUBBER SEED MEAL AS AN ENERGY AND PROTEIN SOURCE FOR RATS FED SOYBEAN MEAL- OR PEANUT MEAL-SUPPLEMENTED DIETS

1974 ◽  
Vol 54 (2) ◽  
pp. 239-246 ◽  
Author(s):  
E. J. OROK ◽  
J. P. BOWLAND
Keyword(s):  
Soybean Meal ◽  
Nutritive Value ◽  
Peanut Meal ◽  
Carcass Composition ◽  
Sprague Dawley ◽  
Protein Levels ◽  
Nitrogen Free Extract ◽  
Sprague Dawley Rats ◽  
Gain Energy ◽  
Gross Energy

Proximate, amino acid and fatty acid analyses were conducted on a sample of para rubber (Hevea brasiliensis) seeds (RS) obtained from Nigeria. The nutritive value of RS meal (RSM) for weanling Sprague-Dawley rats was tested with soybean meal (SBM)- or peanut meal (PNM)-supplemented diets fed at three protein levels (20, 16 and 12%), by including in the diets 7.4 to 12.4% meal prepared from either fresh or autoclaved decorticated RS. The feeding period was 4 wk, in the last week of which energy and nitrogen digestibilities were determined, followed by carcass analyses for protein, fat and ash. Kernels represented 57.0% of undecorticated RS. Meal from decorticated RS contained 3.9% moisture, 6.50 kcal gross energy/g, 43.3% crude fat, 18.3% crude protein, 3.8% crude fiber, 3.1% ash and 27.5% nitrogen-free extract. Rate of gain, energy and nitrogen digestibilities and carcass composition of rats fed diets containing RSM were comparable to those on the respective diets without RSM. Average food intake of rats fed RSM-supplemented diets was lower (P < 0.01) than that of rats on the SBM-supplemented diet. This lower consumption of diets containing RSM was consistent with their higher DE and ME (kcal/g food). Autoclaving RS had no consistent effects on the various measurements.

NIGERIAN COCOA HUSKS AND CASSAVA MEAL AS SOURCES OF ENERGY FOR RATS FED SOYBEAN MEAL- OR PEANUT MEAL-SUPPLEMENTED DIETS

1974 ◽  
Vol 54 (2) ◽  
pp. 229-238 ◽  
Author(s):  
E. J. OROK ◽  
J. P. BOWLAND
Keyword(s):  
Soybean Meal ◽  
Crude Protein ◽  
Peanut Meal ◽  
Protein Supplementation ◽  
Dietary Energy ◽  
Sprague Dawley ◽  
Yellow Corn ◽  
Sprague Dawley Rats ◽  
Overall Performance ◽  
Amino Acid Contents

Nigerian cocoa husks (CH) and cassava meal (CM) were evaluated chemically and biologically in comparison with Canadian yellow corn(C), Canadian soybean meal (SBM) and U.S.A. peanut meal (PNM) using weanling Sprague-Dawley rats. The growth experiment lasted 4 wk. Five major dietary energy-protein combinations were employed, one of which was supplemented with DL-methionine and L-lysine. Each major dietary category was subdivided into three isocaloric (3,600 kcal digestible energy/kg) subgroups containing 20, 16 and 12% crude protein respectively. The crude fat, crude protein and amino acid contents of the foodstuffs and prussic acid contents of CM and cassava root peels, and the theobromine content of detoxified and undetoxified CH were determined. Energy source substantially influenced the response to protein supplementation of the diets. Inclusion of CM at 30 to 50% of the diet gave the best overall performance. Isonitrogenous substitution of dethrobrominized CH for 6.7% corn in the C-PNM diet gave growth response and energy and nitrogen digestibilities which compared favorably with C-PNM or C-PNM supplemented with DL-methionine and L-lysine, thus suggesting that detheobrominized CH could be efficiently utilized in animal diets.

Curcumin Reduces Neuroinflammation and Improves the Impairments of Anesthetics on Learning and Memory by Regulating the Expression of miR-181a-5p

2021 ◽  
pp. 1-9
Author(s):  
Guizhen Liu ◽  
Yuchuan Sun ◽  
Fei Liu
Keyword(s):  
Cognitive Impairment ◽  
Protective Effect ◽  
Learning And Memory ◽  
Cognitive Dysfunction ◽  
Inflammatory Cytokines ◽  
Neuroprotective Effect ◽  
Morris Water Maze Test ◽  
Sprague Dawley ◽  
Protein Levels ◽  
Sprague Dawley Rats

<b><i>Objective:</i></b> The purpose of this study was to explore the role of curcumin (Cur) in isoflurane (ISO)-induced learning and memory dysfunction in Sprague-Dawley rats and further elucidate the mechanism of the protective effect produced by Cur. <b><i>Methods:</i></b> Rat models of cognitive impairment were established by inhaling 3% ISO. The Morris water maze test was used to assess the cognitive function of rats. ELISA and qRT-PCR were used to analyze the protein levels of pro-inflammatory cytokines and expression levels of miR-181a-5p, respectively. <b><i>Results:</i></b> Cur significantly improved the ISO-induced cognitive dysfunction in rats and alleviated the ISO-induced neuroinflammation. miR-181a-5p was overexpressed in ISO-induced rats, while Cur treatment significantly reduced the expression of miR-181a-5p. Overexpression of miR-181a-5p promoted the cognitive impairment and the release of inflammatory cytokines and reversed the neuroprotective effect of Cur. <b><i>Conclusion:</i></b> Cur has a protective effect on ISO-induced cognitive dysfunction, which may be achieved by regulating the expression of miR-181a-5p.

scholarly journals Dexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx Shedding

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Na Cui ◽  
Hao Wang ◽  
Yun Long ◽  
Longxiang Su ◽  
Dawei Liu
Keyword(s):  
Escherichia Coli ◽  
Vascular Endothelium ◽  
Free Water ◽  
Endothelial Glycocalyx ◽  
Sprague Dawley ◽  
Treatment Groups ◽  
Protein Levels ◽  
Sprague Dawley Rats ◽  
The Matrix ◽  
Rat Thoracic Aorta

The aim of this study is to determine the mechanism of sepsis-induced vascular hyperpermeability and the beneficial effect of glucocorticoid in protecting vascular endothelium. Male Sprague-Dawley rats were given either a bolus intraperitoneal injection of a nonlethal dose of LPS (Escherichia coli055:B5, 10 mg/kg, Sigma) or vehicle (pyrogen-free water). Animals of treatment groups were also given either dexamethasone (4 mg/kg, 30 min prior to LPS injection) or the matrix metalloproteinases (MMPs) inhibitor doxycycline (4 mg/kg, 30 min after LPS injection). Both activities and protein levels of MMP-2p<0.001and MMP-9p<0.001were significantly upregulated in aortic homogenates from LPS-treated rats, associated with decreased ZO-1p<0.001and syndecan-1p=0.011protein contents. Both dexamethasone and doxycycline could significantly inhibit MMPs activity and reserve the expressions of ZO-1 and syndecan-1. The inhibition of MMPs by dexamethasone was significantly lower than that by doxycycline, while the rescue of syndecan-1 expression from LPS-induced endotoxemic rat thoracic aorta was significantly higher in the dexamethasone-treated compared to the doxycycline-treatedp=0.03. In conclusion, activation of MMPs plays important role in regulating ZO-1 and syndecan-1 protein levels in LPS mediated endothelial perturbation. Both dexamethasone and doxycycline inhibit activation of MMPs that may contribute to the rescue of ZO-1 and syndecan-1 expression.

scholarly journals Chemical Composition and Nutritive Value of Some Wild-Gathered Tropical Plant Seeds

1996 ◽  
Vol 17 (3) ◽  
pp. 1-4 ◽  
Author(s):  
Ikechukwu E. Ezeagu ◽  
Cornelia C. Metges ◽  
Jürgen Proll ◽  
Klaus J. Petzke ◽  
Akintunde O. Akinsoyinu
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Energy Levels ◽  
Soluble Sugars ◽  
Total Carbohydrate ◽  
Protein Levels ◽  
Plant Seeds ◽  
Tropical Plant ◽  
Gross Energy ◽  
Alternative Sources

As part of the search for alternative sources of food to alleviate hunger, this study reports the approximate total contents of soluble sugars, starch, and gross energy of nine lesser-known wild-gathered plant seeds. High crude protein levels occurred in Lonchocarpus sericeus (28.03%), Albizia zygia (32.90%), and Gliricidia septum (34.15%). High levels of fat occurred in Entandrophragma angolense (59.30%), L. sericeus (34.15%), and Millettia thonningii (30.66%). Low levels of crude protein but high levels of total carbohydrate were found in Diospyros mespiliformis (77.21% carbohydrate), Daneillia ogea (74.32% carbohydrate), and Afzelia belle (53.96% carbohydrate). Starch contents were high in A. zygia (40.46%), D. ogea (69.62%), and A. belle (51.43%). The seeds with high fat content invariably showed high gross energy levels: E. angolense (30.9 kJ/g), L. sericeus (24.37 kJ/g), and M. thonningii (25.12 kJ/g). The results are compared to those for soya bean and other common staples. It is concluded that these less familiar wild seed plants should not be ignored, but further investigation into possible toxic and antinutrient factors, amino acid patterns, digestibility, and fatty acid composition is still required before recommendations are made.

Antiproliferative and Apoptotic Effects of Shemamruthaa, a Herbal Preparation, in 7,12-Dimethylbenz(a)Anthracene-Induced Breast Cancer Rats

2015 ◽  
Vol 20 (4) ◽  
pp. 259-268 ◽  
Author(s):  
Ayyakkannu Purushothaman ◽  
Elumalai Nandhakumar ◽  
Palanivelu Shanthi ◽  
Thiruvaiyaru Panchanatham Sachidanandam
Keyword(s):  
Breast Cancer ◽  
Mammary Carcinoma ◽  
Nuclear Antigen ◽  
Herbal Preparation ◽  
Sprague Dawley ◽  
Protein Levels ◽  
Anticancer Effects ◽  
Sprague Dawley Rats ◽  
Apoptotic Effects ◽  
Proliferating Cell

A herbal preparation, Shemamruthaa (SM), was formulated to investigate the molecular mechanism by which it exhibits anticancer effects in mammary carcinoma bearing rats. Female Sprague-Dawley rats were used for the study, and mammary carcinoma was induced by administration of 7,12-dimethylbenz( a)anthracene, intragastrically. After 3 months of induction period, the rats were treated with SM (400 mg/kg body weight) for 14 days. Our study shows that SM-treated mammary carcinoma rats showed regression in tumor volume with concomitant increase in p53, Bax, caspase-3, and caspase-9 mRNA and protein levels compared with mammary carcinoma–induced rats. Proliferating cell nuclear antigen and anti-apoptotic Bcl-2 were markedly increased in mammary carcinoma–induced rats, whereas the SM treatment significantly decreased the expression of these proteins. The expression pattern of apoptotic signaling molecules analyzed in the present study signifies the therapeutic efficacy of SM against breast cancer.

Hindlimb unweighting alters endothelium-dependent vasodilation and ecNOS expression in soleus arterioles

2000 ◽  
Vol 89 (4) ◽  
pp. 1483-1490 ◽  
Author(s):  
William G. Schrage ◽  
Christopher R. Woodman ◽  
M. Harold Laughlin
Keyword(s):  
Sodium Nitroprusside ◽  
Group Treatment ◽  
Day Treatment ◽  
Weight Bearing ◽  
Immunoblot Analysis ◽  
Sprague Dawley ◽  
Resistance Arteries ◽  
Protein Levels ◽  
Sprague Dawley Rats ◽  
Mrna And Protein Expression

The purpose of this study was to test the hypothesis that endothelium-dependent dilation is impaired in soleus resistance arteries from hindlimb-unweighted (HLU) rats. Male Sprague-Dawley rats (300–350 g) were exposed to HLU ( n = 14) or weight-bearing control (Con, n = 14) conditions for 14 days. After the 14-day treatment period, soleus first-order (1A) arterioles were isolated and cannulated with micropipettes to assess vasodilator responses to an endothelium-dependent dilator, ACh (10−9–10−4 M), and an endothelium-independent dilator, sodium nitroprusside (SNP, 10−9–10−4 M). Arterioles from HLU rats were smaller than Con arterioles (maximal passive diameter = 140 ± 4 and 121 ± 4 μm in Con and HLU, respectively) but developed similar spontaneous myogenic tone (43 ± 3 and 45 ± 3% in Con and HLU, respectively). Arteries from Con and HLU rats dilated in response to increasing doses of ACh, but dilation was impaired in arterioles from HLU rats ( P = 0.03), as was maximal dilation to ACh (85 ± 4 and 65 ± 4% possible dilation in Con and HLU, respectively). Inhibition of nitric oxide (NO) synthase (NOS) with N ω-nitro-l-arginine (300 μM) reduced ACh dilation by ∼40% in arterioles from Con rats and eliminated dilation in arterioles from HLU rats. The cyclooxygenase inhibitor indomethacin (50 μM) did not significantly alter dilation to ACh in either group. Treatment with N ω-nitro-l-arginine + indomethacin eliminated all ACh dilation in Con and HLU rats. Dilation to sodium nitroprusside was not different between groups ( P = 0.98). To determine whether HLU decreased expression of endothelial cell NOS (ecNOS), mRNA and protein levels were measured in single arterioles with RT-PCR and immunoblot analysis. The ecNOS mRNA and protein expression was significantly lower in arterioles from HLU rats than in Con arterioles (20 and 65%, respectively). Collectively, these data indicate that HLU impairs ACh dilation in soleus 1A arterioles, in part because of alterations in the NO pathway.

scholarly journals Ultrastructural Observations of Chronic Progressive Nephrosis in the Sprague-Dawley Rat

1974 ◽  
Vol 11 (2) ◽  
pp. 153-164 ◽  
Author(s):  
J. E. Gray ◽  
R. N. Weaver ◽  
A. Purmalis
Keyword(s):  
Epithelial Cells ◽  
Granular Material ◽  
Basement Membranes ◽  
Common Disease ◽  
Sprague Dawley ◽  
Paraffin Sections ◽  
Protein Levels ◽  
Dense Bodies ◽  
Sprague Dawley Rats ◽  
Bowman's Capsule

The kidneys of 43 male Sprague-Dawley rats, mostly 12-18 months old, were examined by electron microscopy. Paraffin sections stained with the allochrome procedure and thick epoxy sections stained with toluidine blue were used to select sites of nephrotic changes. Protein levels in urine were monitored periodically. The basement membranes of affected nephrons showed a two- to threefold thickening in the capillary loops and Bowman's capsule. Epithelial cells in the glomeruli underwent compensatory changes of hypertrophy, fusion of foot processes and occasionally microvillar transformation. Dense granular material as well as protein absorption droplets formed in the epithelial cells. Epithelial cells of proximal convolutions atrophied and disappeared as the basement membrane thickened to several micrometers. An increase of dense bodies, granular material, and focal cytoplasmic degradation was observed in these cells. The ongoing irreversible deterioration of scattered nephrons suggested the designation of chronic progressive nephrosis for this common disease of Sprague-Dawley rats over 1 year old.

A Maternal Low-protein Diet during Gestation Induces Hepatic Autophagy-related Gene Expression in a Sex-specific Manner in Sprague-Dawley Rats

2021 ◽  
pp. 1-29
Author(s):  
Mingzhu Cai ◽  
Jie Zhang ◽  
Hong Chen ◽  
Yuan-Xiang Pan
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Control Diet ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Related Gene ◽  
Sprague Dawley ◽  
Protein Levels ◽  
Low Protein ◽  
Sprague Dawley Rats

Abstract This study investigates the mechanism by which maternal protein restriction induces hepatic autophagy-related gene expression in the offspring of rats. Pregnant Sprague-Dawley rats were fed either a control diet (C, 18% energy from protein) or a low-protein diet (LP, 8.5% energy from protein) during gestation, followed by the control diet during lactation and post-weaning. Liver tissue was collected from the offspring at postnatal day 38 and divided into four groups according to sex and maternal diet (F-C, F-LP, M-C, and M-LP) for further analysis. Autophagy-related mRNA and protein levels were determined by real-time PCR and Western blotting, respectively. In addition, chromatin immunoprecipitation (ChIP) was performed to investigate the interactions between transcription factors and autophagy-related genes. Protein levels of p-eIF2a and ATF4 were increased only in the female offspring born to dams fed the LP diet. Correlatively, the mRNA expression of hepatic autophagy-related genes including Map1lc3b, P62/Sqstm1, Becn1, Atg3, Atg7, and Atg10 was significantly greater in the F-LP group than in the F-C group. Furthermore, ChIP results showed greater ATF4 and C/EBP homology protein (CHOP) binding at the regions of a set of autophagy-related genes in the F-LP group than in the F-C group. Our data demonstrated that a maternal LP diet transcriptionally programmed hepatic autophagy-related gene expression only in female rat offspring. This transcriptional program involved the activation of the eIF2α/ATF4 pathway and intricate regulation by transcription factors ATF4 and CHOP.

scholarly journals ENERGY AND PROTEIN INGREDIENTS FOR USE IN PIAPARA (Megaleporinus obtusidens) DIETS: NUTRITIVE VALUE AND INTESTINAL MORPHOMETRY

2021 ◽  
Vol 47 ◽  
Author(s):  
Renata Akemi TANAKA ◽  
Viviane do Nascimento Santana de ALMEIDA ◽  
Carolina Vasconcelos Tavares de FARIAS ◽  
Luana Camargo SOUSA ◽  
Gabriela Castellani CARLI ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Crude Protein ◽  
Nutritive Value ◽  
Corn Germ ◽  
Intestinal Morphology ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Corn Gluten ◽  
Gross Energy ◽  
Intestinal Morphometry

The apparent digestibility coefficients for crude protein (ADCCP), dry matter (ADCDM), and gross energy (ADCGE) of ingredients were determined for piapara (Megaleporinus obtusidens). Test diets were formulated to contain 69.5% of reference diet, 0.5% of chromium oxide, and 30% of test ingredients. The protein ingredients evaluated were tilapia processing residue meal (TPRM), feather and poultry blood meal (FPBM), poultry by-product meal (PBM), meat and bone meal (MBM), cottonseed meal (CM), corn gluten meal (CGM), and soybean meal (SM); the energy ingredients tested were corn (C), corn germ meal (CGRM), rice meal (RM), wheat bran (WB), and sorghum (S). Groups of 30 piaparas were fed twice daily during five days with test diets. Intestinal morphometry of fish were also evaluated. Digestibility coefficients of protein and energy ingredients were highest for soybean meal (ADCDM = 85.8%; ADCCP = 95.2%; and ADCGE = 87.2%) and corn (ADCDM = 94.5%; ADCCP = 76.2%; and ADCGE = 89.3%), respectively. Of the energy test ingredients analyzed, corn had the highest digestibility coefficients and induced beneficial changes on intestinal morphology compared to sorghum and corn germ meal. All protein ingredients showed potential for use in piapara diets, except meat and bone meal .

Locally produced cottonseed meal for growing pigs

1971 ◽  
Vol 11 (49) ◽  
pp. 156 ◽  
Author(s):  
ES Batterham ◽  
FS Shenstone
Keyword(s):  
Fatty Acids ◽  
Cottonseed Meal ◽  
Soybean Meal ◽  
Dry Matter ◽  
Nutritive Value ◽  
Growing Pigs ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Digestible Energy ◽  
Gross Energy

The nutritive value of locally produced cottonseed meal, incorporated at a level of 10 per cent in wheat based diets, was compared with meat and bone meal and soybean meal for growing pigs. The effect of adding synthetic lysine to the cottonseed meal diet was also observed. The diets were fed at restricted rates and pig performance was assessed over the 18 to 45 kg, 45 to 73 kg, and 18 to 73 kg growth phases. Cottonseed meal was of similar nutritive value to meat and bone meal and the addition of lysine increased growth and feed conversion during the 18 to 45 kg growth phase. Soybean meal produced similar gains to the cottonseed meal plus lysine diet during the 18 to 45 kg range, but greater gains than the other three diets over the 18 to 73 kg range. Carcase quality was similar for all four diets. With cottonseed meal there was an increase in the ratio of saturated to unsaturated fatty acids in the backfat of the pigs due to cyclopropene fatty acids in the residual oil in the meal. The metabolizable energy values of the diets were approximately 96.7 per cent of the digestible energy values and there was a significant correlation between them (r = 0.94). The correlation between digestible energy values estimated by the methods (gross energy in the feed X dry matter digestibility) and (gross energy in feed - gross energy in faeces/dry matter intake) was significant (r = 0.92).

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