Glucose, alpha-amino nitrogen, and amino acid exchange across the hindlimb in young double-muscled type bulls maintained at two growth rates

1996 ◽  
Vol 76 (2) ◽  
pp. 193-202 ◽  
Author(s):  
J. L. Hornick ◽  
C. Van Eenaeme ◽  
S. Gauthier ◽  
P. Baldwin ◽  
L. Istasse
Keyword(s):  
Amino Acids ◽  
Growth Rate ◽  
Blood Flow ◽  
Soybean Meal ◽  
Rapid Growth ◽  
Amino Nitrogen ◽  
Vena Cava ◽  
High Growth ◽  
Essential Amino Acids ◽  
Protein Supplementation

The effect of growth rate and protein supplementation on muscle metabolism of eight bulls from the Belgian Blue breed, double-muscled type, was investigated by the arterio-venous difference technique. A low growth (LG) group was maintained at a low growth rate over 36 d, and a rapid growth (RG) group for 28 d before receiving a fattening diet allowing for a rapid growth. At the end of the RG period the RG bulls received a supplement of protected soybean meal. Animals were fitted with an aortic ultrasonic blood flow probe and with catheters in the aorta and the vena cava. The blood flow in the hindlimbs of bulls varied greatly by time of the day but was higher in the RG group. The RG group had a higher arterio-venous difference (AVD) and uptake of alpha-amino nitrogen while AVD in essential amino acids was four times higher and uptake eight times higher. Significant higher AVD or uptake was observed in individual amino acids such as leucine, isoleucine and lysine. The supplementation with protected soybean meal had significant negative effect on the uptake of several amino acids. It was concluded that caution should be exercised when measuring punctually blood flow in muscle tissue, for example by dilution techniques. At high growth rate, the requirements for amino acids are larger than for glucose. Excess protein provides no additional benefit. Key words: Bull, hindlimb catheterism, growth rate, protected soy bean, metabolite

scholarly journals Modification of secondary products of processing triticale into starch with a new strain of the fungus Geotrichium candidum

2020 ◽  
Vol 224 ◽  
pp. 04033
Author(s):  
V V Kolpakova ◽  
R V Ulanova ◽  
D S Kulikov
Keyword(s):  
Amino Acids ◽  
Growth Rate ◽  
High Growth ◽  
Essential Amino Acids ◽  
Culture Liquid ◽  
Geotrichum Candidum ◽  
High Growth Rate ◽  
Ribosomal Genes ◽  
Secondary Products ◽  
Efficient Separation

The aim of this work was to study the possibility of using a new strain of the fungus Geotrichum candidum for the bioconversion of serum remaining after the isolation of starch and proteins from triticale grain. The fungus strain Geotrichum candidum 977 was isolated from the steep waters of triticale grains formed during the production of starch and identified on the basis of analysis of the sequence of ribosomal genes. The strain was characterized by large cells efficient separation of biomass from the culture liquid and high growth rate. On protein-free whey, which remains after isolating proteins from steeping waters the fungus assimilated glucose, maltotriose, fructose and did not assimilate maltose. At a pH of 5.0 the growth of the fungus was not observed at a pH of 5.5 to 6.5 it was weak at a pH of 7.5 to 8.5, the productivity of the fungus increased 1.8 times and amounted to 3.00-3,15 g / 100 cm3. During growth the strain alkalized the medium from pH 5.5 to pH 8.5. The microbial-plant concentrate contained 33.3 ± 2.1% protein and 19 amino acids with a predominance of alanine, aspartic, glutamic acids, lysine, threonine and leucine. The score of essential amino acids exceeded 100% with the exception of sulfurcontaining ones (64 -72%). Thus, the possibility of using a new strain of the fungus G. Candidum 977 for the utilization of wastewater generated during the production of starch and proteins from triticale grains has been shown to obtain fodder protein concentrates.

MATHEMATICAL MODELS FOR THE SYNTHESIS OF PLANT-BASED COMPOSITIONS WITH IMPROVED AMINO ACID COMPOSITION

2021 ◽  
Author(s):  
Irina Gaivoronskaya ◽  
Valenitna Kolpakova
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Mathematical Models ◽  
Functional Properties ◽  
Amino Nitrogen ◽  
Essential Amino Acids ◽  
Major Allergen ◽  
Structural Features ◽  
Covalent Bonds ◽  
Pea Protein

The aim of the work was to optimize the process of obtaining multicomponent protein compositions with high biological value and higher functional properties than the original vegetable protein products. Was realized studies to obtain biocomposites on the base of pea protein-oat protein and pea protein-rice protein. Developed composites were enriched with all limited amino acids. For each of the essential amino acids, the amino acid score was 100% and higher. Protein products used in these compositions are not in major allergen list, which allows to use these compositions in allergen-free products and specialized nutrition. To determine biosynthesis parameters for compositions from pea protein and various protein concentrates with the use of transglutaminase enzyme, was studied effect of concentration and exposition time on the amount of amino nitrogen released during the reaction. Decreasing of amino nitrogen in the medium indicated the occurrence of a protein synthesis reaction with the formation of new covalent bonds. Were determined optimal parameters of reaction: the hydromodule, the exposure time, the concentration of EP of the preparation, were obtained mathematical models. Studies on the functional properties of composites, the physicochemical properties of the proteins that make up their composition, and structural features will make it possible to determine the uses in the manufacture of food products based on their ability to bind fat, water, form foam, gels, and etc.

Supplementation of a low protein diet in an attempt to optimize egg mass output

1991 ◽  
Vol 71 (1) ◽  
pp. 211-220 ◽  
Author(s):  
John D. Summers ◽  
J. L. Atkinson ◽  
D. Spratt
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Soybean Meal ◽  
Egg Production ◽  
Corn Oil ◽  
Control Diet ◽  
Essential Amino Acids ◽  
Egg Mass ◽  
Minimum Requirement ◽  
Low Protein

Pullets were brought into production on a low-protein corn, soybean meal diet to which various nutrients including essential amino acids, choline, corn oil and feathermeal were supplemented. Production commenced between 18 and 20 wk of age and up to at least 60% production, hens fed the low protein diets (10% CP) produced at least as many eggs and as great an egg mass as these given a 17% protein corn, soybean meal control diet. Pullets fed the corn, soybean meal test diets, peaked at around 80% as compared to slightly over 90% for the control. Egg production immediately fell for birds fed the test diets to around 70% for most of the test treatments. Body weight remained constant or fell after peak production for birds fed the test diets as compared to a normal increase for birds on the control diet. Egg size increased for the test diets at a rate which was comparable to that of the control birds. In a second experiment, with older hens, supplementation of the 10% protein test diet with methionine, lysine, arginine and tryptophan, resulted in intakes of these amino acids which met NRC minimum requirement levels. However, egg mass output was reduced approximately 11% compared to the 17% protein control diet. While intakes of several essential amino acids fell below requirement levels, the degree that valine was calculated to be deficient in both experiments corresponded closely with the reduction in egg mass output of hens fed the test as compared to the control diet. Key words: Lysine, methionine, egg weight, body weight, hens.

The uptake of amino acids by mouse cells (strain LS) during growth in batch culture and chemostat culture: the influence of cell growth rate

1967 ◽  
Vol 168 (1013) ◽  
pp. 421-438 ◽  
Keyword(s):  
Amino Acids ◽  
Growth Rate ◽  
Amino Acid ◽  
Cell Growth ◽  
Glutamic Acid ◽  
Batch Culture ◽  
Amino Acid Uptake ◽  
Essential Amino Acids ◽  
Growth Yields ◽  
Acid Uptake

The uptake of thirteen essential amino acids by mouse LS cells in suspension culture was determined by bacteriological assay methods. Chemostat continuous-flow cultures were used to determine the effect of different cell growth rates on the quantitative amino acid requirements for growth. The growth yields of the cells ( Y = g cell dry weight produced/g amino acid utilized) were calculated for each of the essential amino acids. A mixture of the non-essential amino acids, serine, alanine and glycine increased the cell yield from the essential amino acids. The growth yields from nearly all the essential amino acids in batch culture were increased when glutamic acid was substituted for the glutamine in the medium. The growth yields from the amino acids in batch culture were much less at the beginning than at the end of the culture. The highest efficiencies of conversion of amino acids to cell material were obtained by chemostat culture. When glutamic acid largely replaced the glutamine in the medium the conversion of amino acid nitrogen to cell nitrogen was 100 % efficient (that is, the theoretical yield was obtained) at the optimum growth rate (cell doubling time, 43 h). The maximum population density a given amino acid mixture will support can be calculated from the data. It is concluded that in several routinely used tissue culture media the cell growth is limited by the amino acid supply. In batch culture glutamine was wasted by (1) its spontaneous decomposition to pyrrolidone carboxylic acid and ammonia, and (2) its enzymic breakdown to glutamic acid and ammonia, but also glutamine was used less efficiently than glutamic acid. Study of the influence of cell growth rate on amino acid uptake rates per unit mass of cells indicated that a marked change in amino acid metabolism occurred at a specific growth rate of 0.4 day -1 (cell doubling time, 43 h). With decrease in specific growth rate below 0.4 day -1 there was a marked stimulation of amino acid uptake rate per cell and essential amino acids were consumed increasingly for functions other than synthesis of cell material.

The effect of starvation and low nitrogen intakes on the concentration of free amino acids in the blood plasma and on the nitrogen metabolism in sheep

1970 ◽  
Vol 21 (5) ◽  
pp. 723 ◽  
Author(s):  
J Leibholz
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Dry Matter ◽  
Control Diet ◽  
Amino Nitrogen ◽  
Essential Amino Acids ◽  
Isoleucine Leucine ◽  
Plasma Urea ◽  
Low Nitrogen

Crossbred wethers were given a control diet (8 g nitrogen, 730 g dry matter daily) or a low nitrogen diet (0.5 g nitrogen, 520 g dry matter daily) or starved, for a 12 or 20 day experimental period. The concentrations of free serine, glutamine, glycine, alanine, histidine, and arginine in the plasma of the starved sheep decreased significantly while the concentrations of lysine, 3-methylhistidine, and isoleucine increased significantly. The ratio of essential to non-essential amino acids increased from 0.35 to 0.56 in the starved sheep. In sheep on the low nitrogen diet, the ratio of essential to non-essential amino acids in the plasma decreased from 0.40 to 0.27, with significant increases in the concentrations of glutanlic acid, glutamine, glycine, isoleucine, leucine, and 3-methylhistidine. Starvation and the low nitrogen diet both resulted in a reduction of the plasma urea concentrations. Starvation and the low nitrogen diet resulted in a 20-50 % reduction in the flow of saliva and a 40-78% increase in the concentration of total nitrogen. This resulted in no significant change in the daily secretion of nitrogen in the saliva. The concentration of urea in the saliva was increased by 3-54%. The concentrations of individual free amino acids in saliva are reported. The nitrogen content of the rumen was reduced, and after 7 days of starvation or on the low nitrogen diet all rumen nitrogen could be attributed to ammonia and free �-amino nitrogen.

scholarly journals Nutritional interventions in sarcopenia: a critical review

2015 ◽  
Vol 74 (4) ◽  
pp. 378-386 ◽  
Author(s):  
Mary Hickson
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Muscle Mass ◽  
Essential Amino Acid ◽  
Essential Amino Acids ◽  
Protein Supplementation ◽  
Nutrition Intervention ◽  
Nutritional Interventions ◽  
Intervention Trials ◽  
Nutritional Studies

The aim of the present paper is to critically review the details of the published nutrition intervention trials, with and without exercise, targeting sarcopenia. Sarcopenia is the loss of muscle mass, strength and/or performance with age. Since amino acids and energy are required for muscle synthesis it is possible that nutritional intake influences sarcopenia. Nutritional studies are challenging to carry out because of the complexity of modulating dietary intake. It is very difficult to change one nutrient without influencing many others, which means that many of the published studies are problematic to interpret. The studies included evaluate whole protein, essential amino acids and β-hydroxyl β-methylbutyrate (HMB). Whole-protein supplementation failed to show a consistent effect on muscle mass, strength or function. This can be explained by the variations in study design, composition of the protein supplement and the failure to monitor voluntary food intake, adherence and baseline nutritional status. Essential amino-acid supplements showed an inconsistent effect but there are only two trials that have significant differences in methodology and the supplement used. The HMB studies are suggestive of a beneficial effect on older adults, but larger well-controlled studies are required that measure outcomes relevant to sarcopenia, ideally in sarcopenic populations. The issues of timing and distribution of protein intake, and increased splanchnic amino-acid sequestration are discussed, and recommendations for future trials are made.

High protein wheats for poultry. 1. Use in broiler diets

1971 ◽  
Vol 11 (53) ◽  
pp. 619 ◽  
Author(s):  
W Turner ◽  
GG Payne
Keyword(s):  
Amino Acids ◽  
Growth Rate ◽  
Amino Acid ◽  
Crude Protein ◽  
Maximum Level ◽  
Essential Amino Acids ◽  
High Protein ◽  
Amino Acid Supplementation ◽  
Dietary Factor ◽  
Protein Diets

High protein wheat was the sole cereal in 20 and 25 per cent crude protein broiler starter diets. On the. 25 per cent protein diet, performance was maximized without amino acid supplementation. Using high protein wheat in 20 per cent protein diets, growth rate was improved by l-lysine supplementation of 0.3 per cent. However, this growth rate was not at a maximum level. Some other dietary factor was necessary, and this did not appear to be essential amino acids, singly or in combination.

Nitrogen nutrition and regulation of cephalosporin production in Streptomyces clavuligerus

1979 ◽  
Vol 25 (1) ◽  
pp. 61-67 ◽  
Author(s):  
Yair Aharonowitz ◽  
Arnold L. Demain
Keyword(s):  
Amino Acids ◽  
Growth Rate ◽  
Nitrogen Nutrition ◽  
Antibiotic Production ◽  
Inorganic Nitrogen ◽  
Amino Nitrogen ◽  
Nitrogen Sources ◽  
Streptomyces Clavuligerus ◽  
Defined Medium ◽  
Repressive Effect

When used as sole nitrogen source, certain amino acids (e.g., proline, asparagine) supported both growth and sporulation by Streptomyces clavuligerus streaked onto solid defined medium. Ammonium supported growth but suppressed sporulation. Amino nitrogen was best for cephalosporin production in liquid defined medium, although urea was almost as useful. A comparison of amino acids showed asparagine and glutamine to be the best nitrogen sources and arginine to be almost as good. Ammonium salts supported a somewhat lower growth rate than asparagine, but antibiotic production was very poor on these inorganic nitrogen sources. Addition of ammonium to asparagine did not affect growth rate but increased mycelial mass; cephalosporin production was reduced by about 75%. Antibiotic production was more closely associated with growth in the absence of ammonium than in its presence, indicating a strong inhibitory and (or) repressive effect of NH4+ on antibiotic production. Ammonium exerted its negative effect when added at 24 h or earlier, i.e. before antibiotic formation began.

EFFECT OF STEAM PELLETING AND REGRINDING ON THE DIGESTIBILITY OF PROTEIN IN CEREAL GRAINS, SOYBEAN MEAL AND WHEAT BRAN BY THE RAT

1968 ◽  
Vol 48 (1) ◽  
pp. 35-39 ◽  
Author(s):  
E. M. Olsen ◽  
S. J. Slinger
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Wheat Bran ◽  
Soybean Meal ◽  
Protein Digestibility ◽  
Essential Amino Acids ◽  
Cereal Grains ◽  
Protein Utilization ◽  
Amino Acid Availability ◽  
Net Protein

The effect if steam pelleting and regrinding on digestibility of protein in corn, wheat, barley, oats, soybean meal and wheat bran was tested with rats. Percentage amino acid absorption and net protein utilization (NPU) were determined for the wheat bran. Pelleting and regrinding improved the digestibility of protein in bran but had no effect on the digestibility of protein in the other ingredients tested. Increased absorption of amino acids caused by the increased digestibility of protein in bran varied considerably for individual amino acids, being greatest for isoleucine, lysine, methionine and threonine of the essential amino acids. The improvement in protein digestibility and amino acid availability was reflected in a higher NPU.

scholarly journals Optimization of Soybean Meal Fermentation for Aqua-Feed with Bacillus subtilis natto Using the Response Surface Methodology

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 306
Author(s):  
Yukun Zhang ◽  
Manabu Ishikawa ◽  
Shunsuke Koshio ◽  
Saichiro Yokoyama ◽  
Serge Dossou ◽  
...  
Keyword(s):  
Amino Acids ◽  
Response Surface Methodology ◽  
Bacillus Subtilis ◽  
Response Surface ◽  
Layer Thickness ◽  
Soybean Meal ◽  
Essential Amino Acids ◽  
Total Phenolic ◽  
Bacillus Subtilis Natto ◽  
Substrate Ratio

This study aimed to improve the nutritional value of soybean meal (SBM) by solid-state fermentation (SSF) using Bacillus subtilis natto (B. s. natto) to overcome the limitations of SBM usage in aquafeed. The response surface methodology (RSM) was employed to explore the relationships of fermentation conditions, such as temperature, time, water-substrate ratio, and layer thickness, on the degree of protein hydrolysis (DH) and the crude protein (CP) content. The optimum conditions for achieving the higher DH (15.96%) and CP (55.76%) were 43.82 °C, 62.32 h, 1.08 of water-substrate ratio, and a layer thickness of 2.02 cm. CP and DH in the fermented soybean meal (FSM) increased by 9.8% and 177.1%, respectively, and crude fiber decreased by 14.1% compared to SBM. The protein dispersibility index (PDI) decreased by 29.8%, while KOH protein solubility (KPS) was significantly increased by 17.4%. Flavonoids and total phenolic acid content in FSM were increased by 231.0% and 309.4%, respectively. Neutral protease activity (NPA) also reached a high level (1723.6 U g−1). Total essential amino acids (EAA) in FSM increased by 12.2%, higher than the 10.8% increase of total non-essential amino acids (NEAA), while the total free amino acids content was 12.76 times higher than that of SBM. Major anti-nutritional factors in SBM were significantly reduced during the process, and almost all SBM protein macromolecules were decomposed. Together with the cost-effectiveness of SSF, B. s. natto-fermented SBM products have great potential to improve the plant composition and replace high-cost ingredients in aquafeed, contributing to food security and environmental sustainability.

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