New approaches for biomarker stability determination in regulated bioanalysis: trending, bridging and incurred samples

Bioanalysis ◽  
2019 ◽  
Vol 11 (20) ◽  
pp. 1837-1844 ◽  
Author(s):  
Sumit Kar ◽  
Clarinda Islam
Keyword(s):  
Statistical Analysis ◽  
Protease Activity ◽  
Respiratory Diseases ◽  
Complex Matrix ◽  
New Approaches ◽  
Disease States ◽  
Analysis Methodology ◽  
Sample Stability ◽  
The Stability ◽  
Improve Stability

Aim: Determining the stability of biomarkers continues to present challenges. Disease states, complex matrices and differences between recombinant and endogenous analytes require new approaches to maintain stability and measure it. In this report, we determine stability for two assays using trending and statistical analysis. Methodology & results: Monitoring trends helps identify out of specification measurements and determine whether concerns are due to the stability of the analyte. We also describe challenges presented when measuring arginase activity in human sputum, a complex matrix, for respiratory diseases. We controlled preanalytical protease activity and collection heterogeneity and monitored incurred sample stability to improve stability of arginine. Conclusion: These new approaches to achieving and determining biomarker stability may provide solutions for increasingly complex biomarker measurements.

scholarly journals Leaching Behaviour of Construction and Demolition Wastes and Recycled Aggregates: Statistical Analysis Applied to the Release of Contaminants

2021 ◽  
Vol 11 (14) ◽  
pp. 6265
Author(s):  
Alessandra Diotti ◽  
Giovanni Plizzari ◽  
Sabrina Sorlini
Keyword(s):  
Particle Size ◽  
Statistical Analysis ◽  
Raw Materials ◽  
Primary Source ◽  
Recycled Aggregates ◽  
Critical Parameters ◽  
Material Particle ◽  
Analysis Methodology ◽  
Construction And Demolition Wastes ◽  
Leaching Behaviour

Construction and demolition wastes represent a primary source of new alternative materials which, if properly recovered, can be used to replace virgin raw materials partially or totally. The distrust of end-users in the use of recycled aggregates is mainly due to the environmental performance of these materials. In particular, the release of pollutants into the surrounding environment appears to be the aspect of greatest concern. This is because these materials are characterized by a strong heterogeneity which can sometimes lead to contaminant releases above the legal limits for recovery. In this context, an analysis of the leaching behaviour of both CDWs and RAs was conducted by applying a statistical analysis methodology. Subsequently, to evaluate the influence of the particle size and the volumetric reduction of the material on the release of contaminants, several experimental leaching tests were carried out according to the UNI EN 12457-2 and UNI EN 12457-4 standards. The results obtained show that chromium, mercury, and COD are the most critical parameters for both CDWs and RAs. Moreover, the material particle size generally affects the release of contaminants (i.e., finer particles showed higher releases), while the crushing process does not always involve higher releases than the sieving process.

scholarly journals Application of Lipid Class Ratios for Sample Stability Monitoring—Evaluation of Murine Tissue Homogenates and SDS as a Stabilizer

Metabolites ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 277
Author(s):  
Sabrina Krautbauer ◽  
Raquel Blazquez ◽  
Gerhard Liebisch ◽  
Marcus Hoering ◽  
Philip Neubert ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Lipid Class ◽  
High Resolution Mass Spectrometry ◽  
Lipolytic Activity ◽  
Sodium Dodecyl ◽  
Lipid Profiles ◽  
Lipolytic Action ◽  
Sample Stability ◽  
Tissue Homogenates ◽  
The Stability

Lipids are a ubiquitous class of structurally complex molecules involved in various biological processes. In the fast-growing field of lipidomics, preanalytical issues are frequently neglected. Here, we investigated the stability of lipid profiles of murine liver, brain, lung, heart, and spleen homogenates by quantitative flow injection analysis using tandem mass spectrometry and high-resolution mass spectrometry. Storage of tissue homogenates at room temperature showed substantial alterations of the lipid profiles reflecting lipolytic action. Therefore, ratios of ceramide to sphingomyelin, lysophosphatidylethanolamine to phosphatidylethanolamine, lysophosphatidylcholine to phosphatidylcholine, and diglyceride to triglyceride were applied to monitor sample stability and the effect of sodium dodecyl sulfate (SDS) as a potential stabilizing agent. The addition of SDS led to a concentration-dependent stabilization of lipid profiles in liver, brain, and heart homogenates, while in lung and spleen homogenates, in particular, the lysophosphatidylethanolamine to phosphatidylethanolamine ratio increased upon addition of SDS. In conclusion, we demonstrated that lipid class ratios reflecting lipolytic activity could be applied to evaluate both the stability of samples and the influence of stabilizers.

Research on chatter stability in milling and parameter optimization based on process damping

2017 ◽  
Vol 24 (12) ◽  
pp. 2642-2655 ◽  
Author(s):  
Lida Zhu ◽  
Baoguang Liu ◽  
Hongyu Chen
Keyword(s):  
Surface Roughness ◽  
Frequency Method ◽  
Processing Efficiency ◽  
Machined Surface ◽  
Process Damping ◽  
Analysis Methodology ◽  
Optimization Of Process Parameters ◽  
Orthogonal Experiments ◽  
Cutting Chatter ◽  
The Stability

Cutting stability is the prerequisite to ensure efficient and high-precision machining, resulting in poor surface quality and damaged tool, which is the basis for the optimization of process parameters and improvement of processing efficiency. Aiming at process damping caused by interference between a tool flank face and a machined surface of part, the dynamic model and critical condition of stability is proposed in the paper. The frequency method is applied to solve the stability of the cutting chatter, and the correctness of the model is validated by experiments. Moreover, through orthogonal experiments, regression analysis methodology are adopted to establish a prediction model of surface roughness and finally combined with the study findings on milling stability based on process damping and surface roughness, achieved optimization of the milling parameters by genetic optimization algorithm. This conclusion provides a theoretical foundation and reference for the milling mechanism research.

The threat of outflow of individual deposits in systemically important banks of the Russian Federation: A retrospective analysis and regulatory outlook

2021 ◽  
Vol 27 (1) ◽  
pp. 4-21
Author(s):  
Yuliya S. EVLAKHOVA
Keyword(s):  
Statistical Analysis ◽  
Retrospective Analysis ◽  
Russian Federation ◽  
Too Big To Fail ◽  
Bank Run ◽  
Business Operations ◽  
The Russian Federation ◽  
The Stability ◽  
High Threat ◽  
Systemically Important Banks

Subject. The article focuses on the dynamics of individual deposits in the Russian systemically important banks as a factor of the stability of available resources. Objectives. I assess the threat of individual deposits outflowing from the Russian too-big-to-fail banks within 2015–2019. I correspondingly outline recommendations for mitigating the threat in the future. Methods. The study is based on methods of logic, comparative and statistical analysis. I devised and applied our own algorithm for classifying too-big-to-fail banks by threat of individual deposit outflow and its level. Results. Systemically important banks were found to have not been exposed to the high threat of bank run within 2015–2019. Three fundamental credit institutions were constantly exposed to the threat of individual deposit outflow. One of eleven systemic banks continuously demonstrated the low threat of individual deposit outflow. The rest of the banks were migrating among the low-threat and high-threat classes. Conclusions and Relevance. The Russian systemically important banks can refer to our findings to articulate their deposit policy, set and use digital accounts of retail customers. The Bank of Russian can rely on the analysis of the threat of individual deposit outflow and other data on financial and business operations of the banks and its sustainability as part of bank oversight procedures.

Evaluation of the Effect of Fault on the Stability of Underground Workings of Coal Mine through DEM and Statistical Analysis

2018 ◽  
Vol 92 (6) ◽  
pp. 732-742 ◽  
Author(s):  
Arka Jyoti Das ◽  
Prabhat Kumar Mandal ◽  
Satya Prakash Sahu ◽  
Angad Kushwaha ◽  
Rana Bhattacharjee ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Coal Mine ◽  
Underground Workings ◽  
The Stability

scholarly journals Peningkatan Kapabilitas Karyawan dan Penerapan Budaya Baru Perusahaan dalam Mewujudkan Daya Saing

2019 ◽  
Vol 5 (2) ◽  
pp. 239
Author(s):  
Iwan Sudirlan ◽  
M Syamsul Maarif ◽  
Joko Affandi ◽  
Yandra Arkeman
Keyword(s):  
Data Analysis ◽  
Statistical Analysis ◽  
Corporate Culture ◽  
Environmental Changes ◽  
Middle Management ◽  
Survey Method ◽  
Analysis Methodology ◽  
Mission Change ◽  
World Environment ◽  
Descriptive Statistical Analysis

The rapidly changing world environment requires companies to adjust to existing conditions. Environmental changes that occur include technology, information systems, economic and political causes of the emergence of new regulations and derivatives so that industry and industry players in the implementation can run well. One telecommunication company in Indonesia in maintaining revenue and profit growth improves employee capability and application of new corporate culture in realizing competitiveness. A vision and mission change with complete comprehensive transformation with a strategy embodied in employee-driven programs based on company values. The company in transformation years periode from 2011 until 2016 hold engagement survey with main result the majority of opinions like the changes that occur. Researcher use survey method questionnaires to gather information after reborn launched from Indosat Ooredoo employees as respondents that distributed to a level of staff, managers, division heads and group heads. Descriptive statistical analysis methodology is used to analyze information that has been collected. The results data analysis of the questionnaire showed that 7 (seven) variables assessing the improvement of employee capabilities and the application of the company's new culture in realizing competitiveness showed that the seven variables affected the level of security and comfort of employees and middle management.

scholarly journals COVID-19 In United States: Statistical Analysis and Inferences From the Empirical Data

2021 ◽  
Author(s):  
Nivedita Rethnakar
Keyword(s):  
United States ◽  
Statistical Analysis ◽  
Statistical Inference ◽  
Empirical Data ◽  
Respiratory Diseases ◽  
Temporal Dynamics ◽  
The United States ◽  
Mortality Statistics ◽  
Race Ethnicity ◽  
The Impact

Abstract This paper investigates the mortality statistics of the COVID-19 pandemic from the United States perspective. Using empirical data analysis and statistical inference tools, we bring out several exciting and important aspects of the pandemic, otherwise hidden. Specific patterns seen in demo- graphics such as race/ethnicity and age are discussed both qualitatively and quantitatively. We also study the role played by factors such as population density. Connections between COVID-19 and other respiratory diseases are also covered in detail. The temporal dynamics of the COVID-19 outbreak and the impact of vaccines in controlling the pandemic are also looked at with suf- ficient rigor. It is hoped that statistical inference such as the ones gathered in this paper would be helpful for better scientific understanding, policy prepa- ration and thus adequately preparing, should a similar situation arise in the future.

scholarly journals Alveolus Lung-on-a-Chip Platform: A Proposal

Chemosensors ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 248
Author(s):  
Noelia Campillo ◽  
Vinicius Rosa Oliveira ◽  
Renata Kelly da Palma
Keyword(s):  
Human Physiology ◽  
3D Printing ◽  
Respiratory Diseases ◽  
Animal Experiments ◽  
Clinical Applications ◽  
Potential Candidate ◽  
New Approaches ◽  
The World ◽  
New Ideas ◽  
Causes Of Deaths

Respiratory diseases are top-ranked causes of deaths and disabilities around the world, making new approaches to the treatment necessary. In recent years, lung-on-a-chip platforms have emerged as a potential candidate to replace animal experiments because they can successfully simulate human physiology. In this review, we discuss the main respiratory diseases and their pathophysiology, how to model a lung microenvironment, and how to translate it to clinical applications. Furthermore, we propose a novel alveolus lung-on-a-chip platform, based on all currently available methodologies. This review provides solutions and new ideas to improve the alveolar lung-on-a-chip platform. Finally, we provided evidence that approaches such as 3D printing, organ-a-chip devices and organoids can be used in combination, and some challenges could be overcome.

Sample stability: a suggested definition and method of determination.

1976 ◽  
Vol 22 (2) ◽  
pp. 176-183 ◽  
Author(s):  
R E Thiers ◽  
G T Wu ◽  
A H Reed ◽  
L K Oliver
Keyword(s):  
Statistical Design ◽  
Measuring Method ◽  
Chemical Constituent ◽  
Sequential Test ◽  
Method Of Determination ◽  
Sample Stability ◽  
Average Change ◽  
Truncated Normal ◽  
Standard Deviations ◽  
The Stability

Abstract We propose defining the stability of any chemical constituent of stored samples in terms that are quantitatively related to the precision of the measurement by which it is determined. We suggest that a constituent may be considered stable, for a stated period and under exactly defined conditions, when the average change in its measured value is less than a chosen number, K, of standard deviations of the data obtained by the measuring method over the concentration range in question. Based on this definition a technique utilizing a graphical truncated normal sequential test is presented as the appropriate experimental and statistical design for measuring stability. The statistical basis for the proposal is presented.

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