scholarly journals Diet Effects on Growth, Mortality, RNA: DNA Ratio and Gene Expression of Zebrafish Danio rerio

2020 ◽  
Vol 4 (1) ◽  
pp. 19-27
Author(s):  
Md. Golam Rabbane ◽  
Md. Yousuf Ali ◽  
Md. Al Zahid ◽  
Jakir Hossain
Keyword(s):  
Gene Expression ◽  
Growth Hormone ◽  
Weight Gain ◽  
Growth Performance ◽  
Molecular Analysis ◽  
Danio Rerio ◽  
Significant Contribution ◽  
Egg Yolk ◽  
Down Regulation ◽  
Length Gain

This study evaluated the effects of different combination of commercial and natural diets (T1: egg yolk, T2: egg yolk+ Artemia, T3: Horlicks, T4: Horlicks+ Artemia, T5: TetraBits, T6: TetraBits+ Artemia, T7: NovoTom, T8: NovoTom+ Artemia) on growth, mortality, RNA: DNA ratio and gene expression of zebrafish for a period of 45 days. Significantly highest length (2.75±0.04 cm) and weight (0.25±0.10 g) gain were recorded in T2 treatment. Lowest length gain (2.00±0.08 cm) and weight gain (0.14±0.12 g) were calculated in T3. Significant highest mortality (65.00±8.66%) was observed in treatment T7 followed by T5 and T8 and significant lowest mortality (20.00±7.64%) was recorded in T2. By molecular analysis, Highest RNA: DNA ratio was found (1.01) in T5 followed by T4 (0.95) and lowest was found in T1 (0.77). Interestingly, a similar result was observed for gh1 (Growth hormone 1) gene expression. Highest gene expression was found in T5 followed by T4 and lowest in T2. Significant down regulation was observed in T2 for gh1 gene. Finally, the study would suggest that zebrafish can be fed with egg yolk+Artemia for better growth performance. The findings could provide a significant contribution to the field of global zebrafish as well as aquaculture research.

scholarly journals Effects of stocking density on growth of zebrafish (Danio rerio, Hamilton, 1822)

2017 ◽  
Vol 44 (2) ◽  
pp. 209-218 ◽  
Author(s):  
Md Golam Rabbane ◽  
Md Mizanur Rahman ◽  
Md Mizanur Rahman ◽  
Md Anwar Hossain ◽  
Md Anwar Hossain
Keyword(s):  
Weight Gain ◽  
Danio Rerio ◽  
Specific Growth ◽  
Condition Factor ◽  
Stocking Density ◽  
Laboratory System ◽  
Zebra Fish ◽  
Commercial Diet ◽  
The Mean ◽  
Length Gain

The effect of stocking density on growth of zebra fish was examined. Total five different stocking densities (5, 15, 25, 35 and 45 individuals per 2 liters of water) were maintained in triplicate for a period of 60 days. One month old zebrafish were randomly stocked into 15 tanks and fish were fed with commercial diet. The mean weight gain, specific growth rate and length gain for treatment 1 (5 fish/2liter) and treatment 2 (15 fish/2 liter) were significantly (p < 0.05) higher than treatment 3 (25 fish/2 liter), treatment 4 (35 fish/2 liter) and treatment 5 (45 fish/2 liter). The gender weight gain, survival rate and condition factor did not show any significant (p > 0.05) difference among treatments. The result of this study suggests that the stocking density of zebrafish could be 15 fish per 2 litre of water in a laboratory system with aeration.Bangladesh J. Zool. 44(2): 209-218, 2016

Effect of residual feed intake phenotype–nutritional treatment interaction on the growth performance, plasma metabolic variables and somatotropic axis gene expression of growing ewes

2016 ◽  
Vol 56 (10) ◽  
pp. 1593 ◽  
Author(s):  
H. T. Nie ◽  
Z. Y. Wang ◽  
S. Lan ◽  
H. Zhang ◽  
Y. J. Wan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Hormone ◽  
Growth Performance ◽  
Feed Intake ◽  
Mrna Abundance ◽  
Feed Restriction ◽  
Somatotropic Axis ◽  
Nutritional Treatment ◽  
Treatment Interaction ◽  
Ad Libitum

This study aimed to evaluate the effect of residual feed intake (RFI) phenotype and nutritional treatment interaction on the growth performance, plasma variables and gene expression levels within the somatotropic axis. Growing ewes [n = 52, initial bodyweight (BW) = 17.5 ± 0.5 kg, 2 months of age] were offered ad libitum access to diets for 63 days and ranked based on RFI phenotype. Thirty ewes with the highest and lowest RFI values were selected and randomly assigned to three nutritional treatments based on dry matter intake (DMI), which are ad libitum (AL), low restriction (LR) and high restriction (HR) groups, respectively. Each nutritional treatment group included ewes with high (n = 5) and low RFI (n = 5) values. During nutritional treatment (from Day 64 to Day 138), plasma samples were obtained to measure metabolite and hormone concentrations. Tissues of the hypothalamus, pituitary, liver, and Longissimus dorsi muscle (LM) were harvested at the end of the experiment (Day 138) to measure the gene expression level within the somatotropic axis. Muscle growth hormone receptor mRNA abundance of low RFI ewes tended to be greater (P = 0.09) under AL feeding, but this difference was abolished by underfeeding (P > 0.10). Low RFI ewes under HR treatment showed slightly greater growth performance, which was accompanied with lower pituitary somatostain receptor 2 mRNA abundance (P < 0.05), plasma non-esterified fatty acid concentration (P < 0.05), and greater concentration of triglyceride (P < 0.05), compared with ewes classified as high RFI group. Our results suggested that ewes categorised as low RFI showed higher resistance to the condition of high feed restriction, which might be attributed to less intensity of fat mobilisation under negative energy balance. The mechanism underlying resistance to such feed restriction was presumably through action of somatostain receptor 2 and was potentially mediated by inhibitory effects of somatostatin on growth hormone release but not basal growth hormone secretion.

A note on the effect of castration on the growth performance and concentrations of some blood metabolites and hormones in British Friesian male cattle

1978 ◽  
Vol 26 (3) ◽  
pp. 339-342 ◽  
Author(s):  
H. Galbraith ◽  
D. G. Dempster ◽  
T. B. Miller
Keyword(s):  
Fatty Acids ◽  
Growth Hormone ◽  
Weight Gain ◽  
Growth Performance ◽  
Blood Metabolites ◽  
Feed Conversion ◽  
Lean Tissue ◽  
Serum Total Protein ◽  
Serum Growth Hormone ◽  
Plasma Urea

AbstractEight British Friesian bulls and eight steers were compared in terms of performance and levels of metabolites and hormones in circulating blood. Under conditions of generous nutrition the bulls grew significantly more quickly, exhibited a superior feed conversion efficiency and had lower levels of serum albumin and plasma urea and higher levels of serum growth hormone and prolactin than the steers. Differences in levels of plasma glucose and free fatty acids and serum total protein and insulin were not significant. It is suggested that the superior growth performance of the bulls is due, at least partially, to hormonally mediated differences in nitrogen metabolism that result in a greater deposition of lean tissue and hence increased weight gain.

Response to growth hormone treatment in very young patients with growth hormone deficiencies and mini-puberty

2018 ◽  
Vol 31 (2) ◽  
pp. 175-184 ◽  
Author(s):  
Semra Çetinkaya ◽  
Şükran Poyrazoğlu ◽  
Firdevs Baş ◽  
Oya Ercan ◽  
Metin Yıldız ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Weight Gain ◽  
Growth Response ◽  
Gh Deficiency ◽  
Young Patients ◽  
First Year ◽  
Gh Treatment ◽  
Factors Affecting ◽  
Second Year ◽  
Length Gain

Abstract Background: The aim of the study was to assess the response to growth hormone (GH) treatment in very young patients with GH deficiency (GHD) through a national, multi-center study. Possible factors affecting growth response were assessed (especially mini-puberty). Methods: Medical reports of GHD patients in whom treatment was initiated between 0 and 3 years of age were retrospectively evaluated. Results: The cohort numbered 67. The diagnosis age was 12.4±8.6 months, peak GH stimulation test response (at diagnosis) as 1.0±1.4 ng/mL. The first and second years length gain was 15.0±4.3 and 10.4±3.4 cm. Weight gain had the largest effect on first year growth response; whereas weight gain and GH dose were both important factors affecting second year growth response. In the multiple pituitary hormone deficiency (MPHD) group (n=50), first year GH response was significantly greater than in the isolated GH deficiency (IGHD) group (n=17) (p=0.030). In addition first year growth response of infants starting GH between 0 and 12 months of age (n=24) was significantly greater than those who started treatment between 12 and 36 months of age (n=43) (p<0.001). These differences were not seen in the second year. Δ Length/height standard deviation score (SDS), Δ body weight SDS, length/height SDS, weight SDS in MPHD without hypogonadism for the first year of the GH treatment were found as significantly better than MPHD with hypogonadism. Conclusions: Early onsets of GH treatment, good weight gain in the first year of the treatment and good weight gain-GH dose in the second year of the treatment are the factors that have the greatest effect on length gain in early onset GHD. The presence of the sex steroid hormones during minipubertal period influence growth pattern positively under GH treatment (closer to the normal percentage according to age and gender).

scholarly journals Down-regulation of complement genes in lipopolysaccharide-challenged zebrafish (Danio rerio) larvae exposed to Indonesian propolis

2023 ◽  
Vol 83 ◽  
Author(s):  
I. Wibowo ◽  
N. Marlinda ◽  
F. R. Nasution ◽  
R. E. Putra ◽  
N. Utami ◽  
...  
Keyword(s):  
Gene Expression ◽  
Danio Rerio ◽  
Complement System ◽  
Yolk Sac ◽  
Liver Histology ◽  
Histological Structure ◽  
Down Regulation ◽  
Expression Levels ◽  
Zebrafish Larvae ◽  
Complement Gene

Abstract Although propolis has been reported for having anti-inflammatory activities, its effects on complement system has not been much studied. This research was conducted to find out the effects of Indonesian propolis on the expression levels of C3, C1r/s, Bf, MBL, and C6 in zebrafish larvae which were induced by lipopolysaccharide (LPS). Counting of macrophages migrating to yolk sac and liver histology were carried out. Larvae were divided into four groups: CON (cultured in E3 medium only), LPS (cultured in a medium containing 0.5 μg/L LPS), LPSIBU (cultured in a medium containing LPS, and then treated with 100 μg/L ibuprofen for 24 hours), and LPSPRO (cultured in a medium containing LPS, and then immersed in 14,000 μg/L propolis for 24 hours) groups. The results showed that complement gene expression in larvae from the LPSIBU and LPSPRO groups were generally lower than in larvae from the LPS group. The number of macrophage migrations to the yolk in the LPSPRO group was also lower than in the LPS group. Histological structure of liver in all groups were considered normal. This study shows that Indonesian propolis has the potential to be used as an alternative to the substitution of NSAIDs.

Calves sucking colostrum and milk from their dams or from an automatic feeding station starting in the neonatal period: metabolic and endocrine traits and growth performance

2002 ◽  
Vol 74 (3) ◽  
pp. 431-444 ◽  
Author(s):  
G. Schiessler ◽  
A. Nussbaum ◽  
H. M. Hammon ◽  
J. W. Blum
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Weight Gain ◽  
Growth Factor ◽  
Growth Performance ◽  
Total Protein ◽  
Neonatal Period ◽  
Plasma Concentrations ◽  
Group Differences ◽  
Feeding Station

AbstractMetabolic and endocrine traits and growth performance from birth up to day 28 in calves sucking colostrum and milk at a computer-programmed automatic feeding station (GrA, no. = 7) were compared with calves sucking their dams (GrS, no. = 7). Body weight tended to be higher on day 28 in GrS than in GrA (74 (s.e. 4) and 70 (s.e. 2) kg, respectively; P 0·1), but not weight gain from 0 to 28 days. Intakes in GrA increased from days 1 to 4 and then remained at 11·1 (s.e. 1·0) l/day. From days 2 to 11 calves visited the automatic feeding station 9 (s.e. 1) times per day. Plasma concentrations of urea (days 3 and 21), glucagon (day 3) and growth hormone (day 3) were higher in GrA than in GrS (P < 0·05), whereas plasma concentrations of total protein (days 1 to 28), immunoglobulin G (days 1 to 28), albumin (days 1 to 14), glucose (day 3), lactate (days 1 and 28), triglycerides (day 3), cholesterol (days 21 and 28), insulin (day 3), and insulin-like growth factor-1 (day 21) were lower in GrA than in GrS (P < 0·05). Metabolic and endocrine group differences were mainly seen in week 1. However, growth performance during the first 3 weeks of life was comparable in calves of the two groups but resulted in slightly enhanced body weight (by 4 ± 3·5 kg) at the end of the 1st month of life in GrS.

scholarly journals Effects of different supplementary feeds on the growth performance and survival of Labeo bata fry

2018 ◽  
Vol 7 (1) ◽  
pp. 15-21
Author(s):  
Md Baki Billah ◽  
Suny Akter ◽  
Suraiya Parveen ◽  
Azizur Rahman ◽  
Lutfun Nahar ◽  
...  
Keyword(s):  
Weight Gain ◽  
Survival Rate ◽  
Growth Performance ◽  
Wheat Bran ◽  
Specific Growth ◽  
Mixed Diet ◽  
Labeo Bata ◽  
Maize Bran ◽  
Length Gain ◽  
Percent Weight Gain

An experiment was conducted with Labeo bata fry for 60 days to examine the growth performance and survival in response to three supplementary feeds i.e. wheat bran, maize bran and mixed diet. The length gain, percent length gain, weight gain, percent weight gain and specific growth of the fry were found significantly higher (p<0.05) in mixed diet. The highest survival rate (74.38±8.1%) was shown by the mixed diet treated fry which was significantly higher than those of maize bran and wheat bran treated fry respectively. There were no significant differences (p<0.05) among the treatments in term of water temperatures, pH and dissolved oxygen (DO). Thus, on the basis of the fry growth performance and survival rate, it could be suggested that the mixed diet of wheat bran and maize bran is suitable for the culture of L. bata fry.Jahangirnagar University J. Biol. Sci. 7(1): 15-21, 2018 (June)

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