Foliar Application of Saudi Desert Plants Extract Improved Some Mungbean Agronomic Traits Under Drought Stress

To evaluate Saudi desert plants potential to promote mungbean growth under drought stress, a pot experiment was performed at field experimental station, King Abdulaziz University during 2016/2017 season. Drought stress was imposed by application of 40% and 60% field capacity (FC) levels. Pots with 100% field capacity were also maintained as control. Natural plant extracts of ten desert plants (Eucalyptus globulus, Azadirachta indica, Moringa oleifera Lam, Moringa peregrine Forssk., Tetraena simplex L., Simmondsia chinensis, Indigofera tinctoria, Dianella ensata, Emex spinosa, Ambrosia dumosa) were foliar applied prior to stress treatment application. Tap water treatment was used as control. Data for mungbean growth and pod characteristics were recorded. Drought stress decreased mungbean growth and severity of impact increased with lowering field capacity. The natural plant extract (NPE); NPE 9 (Dianella ensata) and NPE 11 (Ambrosia dumosa) favoured both plant growth 33% and pod characteristics 11%. The effect of NPE 6 (Tetraena simplex L.) and NPE 7 (Simmondsia chinensis) were significant for plant growth 21-24 cm while non-significant for pod characteristics under severe drought stress. On contrary, NPE 2 (Eucalyptus globulus) and NPE 3 (Azadirachta indica) effect was significant for mungbean pod characteristics 60% and non-significant for growth traits. The rest of the applied NPEs were non-significant for both growth and yield traits. The combination of NPE9 × FC-2, NPE6 × FC-2, NPE11 × FC-1 and NPE7 × FC-1 produced tallest plants (29.33 cm), maximum fresh biomass (8.54 g), dry biomass (6.71 g) and number of branches per plant (6.8). The combination of NPE2 × FC-2, NPE3 × FC-1, NPE9 × FC-1 and NPE11 × FC-3 produced maximum pods per plant (4.2), pod length (7 cm), pod fresh weight (5.4 g) and dry weight (4.5 g) respectively. It’s clear from the statistical analysis that mungbean performed better under medium stress level (FC-2) while NPE6, NPE7, NPE9 and NPE11 significantly stimulated plant growth under stressed environment. In conclusion, by application of Dianella ensata and Ambrosia dumosa, mungbean can be successfully grown under medium drought stress conditions.

Download Full-text

Legume Plant Growth at Various Levels of Drought Stress Treatment

Indonesian Journal of Agricultural Research ◽

10.32734/injar.v1i1.182 ◽

2018 ◽

Vol 1 (1) ◽

pp. 9-19 ◽

Cited By ~ 1

Author(s):

Veronica Sinamo ◽

Nevy Diana Hanafi ◽

Tri Hesty Wahyuni

Keyword(s):

Drought Stress ◽

Plant Growth ◽

Crop Production ◽

Animal Feed ◽

Field Capacity ◽

Severe Drought ◽

Fresh Matter ◽

Dry Land ◽

Stress Treatment ◽

Matter Production

A strategic alternative step for the development of cultivation of feed crops is to optimize the use of dry land that has the potential for agricultural and livestock business. One of the forage plants that has good adaptation to less good soil condition and is a pioneer plant, Pueraria javanica legume, so it can be considered as a potential alternative forage as animal feed. The research aims at determining the effect of various levels of drought stress treatment on the plant hight, fresh matter production, dry matter production and root biomass of Pueraria javanica legume. The experiment was conducted in the Greenhouse of the Agriculture Faculty of Universitas Sumatera Utara. The experiment was carried out using a completely randomized design (CRD) with 3 treatments and 4 replications. The treatments were A1 (25% field capacity (FC)=144 ml/polybag, A2 (50% FC)=288 ml/polybag and A3 (100% FC)=576 ml/polybag. The results show that Pueraria javanica legume can survive and grow up to severe drought stress (25% FC) but the crop production does not increase when compared to the field capacity condition; besides, the legume cannot survive longer in drought condition. The volume of water supplied corresponding to the field capacity makes the plant growth better.

Download Full-text

Enhancement of drought tolerance in diverse Vicia faba cultivars by inoculation with plant growth-promoting rhizobacteria under newly reclaimed soil conditions

Scientific Reports ◽

10.1038/s41598-021-02847-2 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Elsayed Mansour ◽

Hany A. M. Mahgoub ◽

Samir A. Mahgoub ◽

El-Sayed E. A. El-Sobky ◽

Mohamed I. Abdul-Hamid ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Plant Growth ◽

Plant Growth Promoting Rhizobacteria ◽

Severe Drought ◽

Drought Conditions ◽

Moderate Drought ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Effects Of Drought

AbstractWater deficit has devastating impacts on legume production, particularly with the current abrupt climate changes in arid environments. The application of plant growth-promoting rhizobacteria (PGPR) is an effective approach for producing natural nitrogen and attenuating the detrimental effects of drought stress. This study investigated the influence of inoculation with the PGPR Rhizobium leguminosarum biovar viciae (USDA 2435) and Pseudomonas putida (RA MTCC5279) solely or in combination on the physio-biochemical and agronomic traits of five diverse Vicia faba cultivars under well-watered (100% crop evapotranspiration [ETc]), moderate drought (75% ETc), and severe drought (50% ETc) conditions in newly reclaimed poor-fertility sandy soil. Drought stress substantially reduced the expression of photosynthetic pigments and water relation parameters. In contrast, antioxidant enzyme activities and osmoprotectants were considerably increased in plants under drought stress compared with those in well-watered plants. These adverse effects of drought stress reduced crop water productivity (CWP) and seed yield‐related traits. However, the application of PGPR, particularly a consortium of both strains, improved these parameters and increased seed yield and CWP. The evaluated cultivars displayed varied tolerance to drought stress: Giza-843 and Giza-716 had the highest tolerance under well-watered and moderate drought conditions, whereas Giza-843 and Sakha-4 were more tolerant under severe drought conditions. Thus, co-inoculation of drought-tolerant cultivars with R. leguminosarum and P. putida enhanced their tolerance and increased their yield and CWP under water-deficit stress conditions. This study showed for the first time that the combined use of R. leguminosarum and P. putida is a promising and ecofriendly strategy for increasing drought tolerance in legume crops.

Download Full-text

DEHYDRIN PROFILES OF SOME IRANIAN MELON VARIETIES (Cucumis melo L. Merr) UNDER DROUGHT STRE SS CONDITIONS

Acta Scientiarum Polonorum Hortorum Cultus ◽

10.24326/asphc.2019.6.8 ◽

2019 ◽

Vol 18 (6) ◽

pp. 75-84

Author(s):

Alireza Motallebi-Azar ◽

István Papp ◽

Anita Szegő

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Water Content ◽

Water Status ◽

Field Capacity ◽

Total Protein Content ◽

Severe Drought ◽

Molecular Weights ◽

Potential Marker ◽

Relative Water

Dehydrins are proteins that play a role in the mechanism of drought tolerance. This study aimed at establishing dehydrin profile and accumulation in four local melon varieties of Iran: Mino, Dargazi, Saveii, and Semsori, as well as in a commercial variety Honeydew. Plants were treated with drought stress by adjusting the soil water content to 75, 50, 40, 30 and 20% of field capacity (FC) by withholding water. Water status of plants was monitored based on the seedling fresh weight (FW) and relative water content of leaves (RWC). Total protein content was extracted, then heat-stable protein (HSP) fraction was isolated for each variety and water stress treatment. After SDS-PAGE of HSP, Western blotting analysis was carried out with Anti-dehydrin rabbit (primary) and Goat anti rabbit (secondary) antibodies. ANOVA results showed that with decreasing FC below 75%, FW and RWC decreased, but these changes significantly varied among genotypes. On the basis of FW and RWC data under different drought stress treatments, the following drought-tolerant ranking was established: Mino > Dargazi > Saveii and Honeydew > Semsori, from tolerant to sensitive order. Results of Western blot analysis showed that expression of some proteins with molecular weights of 19–52 kDa was induced in the studied varieties under drought stress (% FC). Expression level of the dehydrin proteins in different varieties was variable and also depending on the drought stress level applied. However, dehydrin proteins (45 and 50 kDa) showed strong expression levels in all varieties under severe drought stress (20% FC). The abundance of dehydrin proteins was higher in tolerant varieties (Mino and Dargazi) than in moderate and drought sensitive genotypes. Consequently, dehydrins represent a potential marker for selection of genotypes with enhanced drought tolerance.

Download Full-text

Physiological and biochemical responses to severe drought stress of nine Eucalyptus globulus clones: a multivariate approach

Tree Physiology ◽

10.1093/treephys/tpu052 ◽

2014 ◽

Vol 34 (7) ◽

pp. 778-786 ◽

Cited By ~ 19

Author(s):

V. Granda ◽

C. Delatorre ◽

C. Cuesta ◽

M. L. Centeno ◽

B. Fernandez ◽

...

Keyword(s):

Drought Stress ◽

Eucalyptus Globulus ◽

Severe Drought ◽

Multivariate Approach ◽

Biochemical Responses ◽

Physiological And Biochemical

Download Full-text

Growth Indices of Kimiya Cultivar of Lentil in Response to Drought Stress at Flowering and Pod Filling Stages under Greenhouse Conditions

Legume Research - An International Journal ◽

10.18805/lr-501 ◽

2019 ◽

Author(s):

Hossein Jahantigh ◽

Seyed Reza Amiri

Keyword(s):

Growth Rate ◽

Drought Stress ◽

Plant Growth ◽

Field Capacity ◽

Flowering Stage ◽

Growth Indices ◽

Relative Growth ◽

Randomized Design ◽

Completely Randomized Design ◽

Net Assimilation

Drought stress is one of the most important environmental factors which can limit the lentil production. To evaluate the effect of drought stress at flowering and pod filling stages on growth indices of lentil cultivar Kimiya (a new high yielding lentil cultivar for moderate cold and semi warm climate of Iran), an experiment was conducted at the research greenhouse of Higher Educational Complex of Saravan in completely randomized design with four replications. The drought stress was applied by withholding irrigation until the soil moisture reached to 20% of field capacity at stages of flowering and pod filling. In this experiment, we measured grain yield per plant, growth indices including plant leaf area (PLA), relative growth rate (RGR), plant growth rate (PGR), and net assimilation rate (NAR). Results showed that the flowering stage in lentil plant is more sensitive to drought as exhibited significant decline in PLA, RGR, PGR and NAR. PGR declined at the end of growing season when vegetative growth stop, senescence of leaves, the reduction of NAR, and the allocation of assimilate to the seeds.

Download Full-text

The Effect of Foliar Application of Melatonin on Changes in Secondary Metabolite Contents in Two Citrus Species Under Drought Stress Conditions

Frontiers in Plant Science ◽

10.3389/fpls.2021.692735 ◽

2021 ◽

Vol 12 ◽

Author(s):

Marziyeh Jafari ◽

Alireza Shahsavar

Keyword(s):

Drought Stress ◽

Secondary Metabolites ◽

Secondary Metabolite ◽

Foliar Application ◽

Plant Secondary Metabolites ◽

Field Capacity ◽

Total Phenolic ◽

Severe Drought ◽

Plant Interactions ◽

Phenolic Contents

Plant secondary metabolites are compounds that play an important role in plant interactions and defense. Persian lime and Mexican lime as the two most important sour lime varieties with high levels of secondary metabolites, are widely cultivated in tropical and subtropical areas. Melatonin is a pleiotropic molecule that plays a key role in protecting plants against drought stress through regulating the secondary metabolite biosynthesis pathway. This study was performed as a factorial experiment consisting of three factors in a completely randomized design (CRD), including four concentrations of melatonin (0, 50, 100, and 150 μM), three levels of drought stress [100% (control), 75% (moderate stress), and 40% (severe stress) field capacity (FC)], and two Citrus cultivars. The experiment was conducted for 60 days in a greenhouse condition. Based on the results of this study under severe drought stress, melatonin-treated crops had higher total flavonoid and total phenolic contents than the untreated crops. The highest level of essential oils components was observed on 100 μM foliar application of melatonin under severe drought stress in both varieties. The main component of the essential oil was limonene in both Citrus species. Moreover, based on the analysis of the results, hesperidin was the main polyphenol in both varieties. Since the use of melatonin often increases the production of secondary metabolites, this study can be considered as a very effective method for controlling the adverse effects of drought stress in citrus for both industrial and horticultural aims.

Download Full-text

Protection Mehcanism Against Drought In Axsonopus Compressus: Insight of Physio-Biochemical Traits, Antioxident Interplay and Gene Experssion

10.21203/rs.2.20552/v1 ◽

2020 ◽

Author(s):

Mohsin Nawaz ◽

Li Liao ◽

Farrukh Azeem ◽

Umair Ashraf ◽

Ali Zohaib ◽

...

Keyword(s):

Drought Stress ◽

Soluble Sugar ◽

Field Capacity ◽

Physiological Data ◽

Severe Drought ◽

Severe Damage ◽

Environmental Constraint ◽

Stunted Growth ◽

Defense Systems ◽

Scarce Water

Abstract Drought is a major environmental constraint that affects plant growth and internal physio-biochemical features. The present study was conducted to evaluate the performance of three different Axonopus compressus accessions, i.e., A-38, A-58, and A-59 under well-watered (WW), low drought (LD), moderate (MD) and severe drought (SD) conditions at field capacity of 100, 80, 60, and 40%, respectively. Results indicated that drought-induced higher production of proline and soluble sugar (SS) up to 40 and 41% respectively, than control. Drought stress caused excessive production of H 2 O 2 while the highest value (10.15µmol g -1 FW) was observed in the A-38 under SD. However, the lowest enzymatic (SOD, POD, CAT, and APX) activity were observed in A-38 than A-58 and A-59 respectively, in the SD. In A-58 the efficient enzymatic and nonenzymatic defense systems hinder the severe damage while stunted growth occurred in Axonopus compressus accessions at SD which were more pronounced in the A-38. Overall, the performance of all Axonopus compressus accessions under drought stress was recorded as A-58>A-59>A-38. The qRT PCR expression analysis also revealed highest expression of drought responsive genes in A-58 and reinforced the findings of physiological data. These results suggested the plant's ability to maintain its functions during drought induction could be used for further investigation under scarce water for developing drought tolerance.

Download Full-text

Effect of Three Water Regimes on the Physiological and Anatomical Structure of Stem and Leaves of Different Citrus Rootstocks with Distinct Degrees of Tolerance to Drought Stress

Horticulturae ◽

10.3390/horticulturae7120554 ◽

2021 ◽

Vol 7 (12) ◽

pp. 554

Author(s):

Waqar Shafqat ◽

Yasser S. A. Mazrou ◽

Sami-ur-Rehman ◽

Yasser Nehela ◽

Sufian Ikram ◽

...

Keyword(s):

Drought Stress ◽

Plant Growth ◽

Moisture Content ◽

Vascular Bundle ◽

Anatomical Structure ◽

Severe Drought ◽

Plant Water ◽

Sour Orange ◽

Cell Area ◽

Cell Thickness

Citrus is grown globally throughout the subtropics and semi-arid to humid tropics. Abiotic stresses such as soil water deficit negatively affect plant growth, physiology, biochemistry, and anatomy. Herein, we investigated the effect(s) of three water regimes (control, moderate drought, and severe drought) on the physiological and anatomical structure of 10 different citrus rootstocks with different degrees of tolerance to drought stress. Brazilian sour orange and Gadha dahi performed well by avoiding desiccation and maintaining plant growth, plant water status, and biochemical characters, while Rangpur Poona nucellar (C. limonia) and Sunki × bentake were the most sensitive rootstocks at all stress conditions. At severe water stress, the highest root length (24.33 ± 0.58), shoot length (17.00 ± 1.00), root moisture content (57.67 ± 1.53), shoot moisture content (64.59 ± 1.71), and plant water potential (−1.57 ± 0.03) was observed in tolerant genotype, Brazilian sour orange. Likewise, chlorophyll a (2.70 ± 0.06), chlorophyll b (0.87 ± 0.06) and carotenoids (0.69 ± 0.08) were higher in the same genotype. The lowest H2O2 content (77.00 ± 1.00) and highest proline content (0.51 ± 0.06) were also recorded by Brazilian sour orange. The tolerance mechanism of tolerant genotypes was elucidated by modification in anatomical structures. Stem anatomy at severe drought, 27.5% increase in epidermal cell thickness, 25.4% in vascular bundle length, 30.5% in xylem thickness, 27.7% in the phloem cell area, 8% in the pith cell area, and 43.4% in cortical thickness were also observed in tolerant genotypes. Likewise, leaf anatomy showed an increase of 27.9% in epidermal cell thickness, 11.4% in vascular bundle length, 21% in xylem thickness, and 15% in phloem cell area in tolerant genotypes compared with sensitive ones. These modifications in tolerant genotypes enabled them to maintain steady nutrient transport while reducing the risk of embolisms, increasing water-flow resistance, and constant transport of nutrients across.

Download Full-text

The Effect of Potassium Addition on Oil Palm (Elaeis guineensis Jacq.) Roots Anatomic Properties under Drought Stress

Caraka Tani Journal of Sustainable Agriculture ◽

10.20961/carakatani.v35i1.32578 ◽

2020 ◽

Vol 35 (1) ◽

pp. 54

Author(s):

Wiski Irawan ◽

Eka Tarwaca Susila Putra

Keyword(s):

Drought Stress ◽

Oil Palm ◽

Elaeis Guineensis ◽

Block Design ◽

Field Capacity ◽

Limiting Factors ◽

Severe Drought ◽

Cell Width ◽

K Fertilizer ◽

Moderate Drought

The availability of water is one of the main limiting factors for oil palm growth and production. Potassium (K) is an essential nutrient for plants because of its role in controlling metabolic and physiological activities. This study aimed to examine the effect of different K fertilizer doses on root anatomic properties under drought stress. The experiment was arranged in factorial Randomized Complete Block Design (RCBD) with two factors. The first factor was drought stress, consisting of three levels of fractions of transpirable soil water (FTSW) (FTSW 1 (control: field capacity); FTSW 0.35 (moderate drought); FTSW 0.15 (severe drought)) and the second factor was K dose (sourced from KCl), consisting of five levels (K0: 0%; K1: 50%; K2: 100%; K3: 150%; K4: 200%). The results showed that there was an interaction between the addition of K doses and the tolerance level of oil palm plants to drought stress. The addition of 100% K gave higher results in the parameters of xylem diameter, phloem diameter and cortex cell width compared to the plants without K. The results disclosed that 200% K application on moderate drought stress and severe drought stress in oil palm seedlings could widen xylem diameter, phloem diameters, strengthen cell such as epidermal cells, cortex cells, thickness of endodermic cells, thickness of sclerenchyma cells and increase hardness of cell compared to field capacity. As for the parameters of thick endodermic cells, stele diameter and sclerenchyma diameter, an addition of 50% K could give higher results.

Download Full-text

The role of calcium in drought stress response induced through antioxidant activity in oil palm (Elaeis guineensis Jacq.) seedlings

E-Journal Menara Perkebunan ◽

10.22302/iribb.jur.mp.v89i1.425 ◽

2021 ◽

Vol 89 (1) ◽

Author(s):

Endah NURWAHYUNI ◽

Eka Tarwaca Susila PUTRA

Keyword(s):

Drought Stress ◽

Oil Palm ◽

Elaeis Guineensis ◽

Essential Element ◽

Field Capacity ◽

Severe Drought ◽

Enzymatic Antioxidants ◽

Drought Treatment ◽

Sod Activity ◽

Effects Of Drought

Oil palm productivity in Indonesia faces challenges related to drought that occur during the dry season. Calcium is an element that plays a role in determining the response of plant resistance to drought through biochemical activity. This study aims to determine the contribution of calcium in biochemical mechanisms involving various antioxidants. The treatment was arranged in factorial of 3 x 4 in a split-plot design. The first factor was calcium dosage, which consisted of 0 g (control/without calcium), 0.04 g, 0.08 g, and 0.12 g of calcium per plant. The second factor was the intensity of drought stress, referred as the Fraction of Transpirable Soil Water (FTSW) at 1 (control/field capacity), 0.35 (moderate drought), and 0.15 (severe drought) with a week duration of intensity. Calcium was applied in a ring placement on four-month-old seedlings planted in 40 x 40 cm polybags with alfisol soil planting medium and given drought treatment two months later for three weeks. The results showed that calcium could induce plant response to drought through the increase in superoxide dismutase (SOD) activity, the decrease in hydrogen peroxide (H₂O₂) concentration, and the decrease in malondialdehyde (MDA) concentration. The study concluded that calcium is an essential element used to reduce the effects of drought on oil palm seedlings through the change of biochemical activities regulated by enzymatic antioxidants.

Download Full-text