scholarly journals Analysis of Genetic Potential of Banyuwangi Local Rice (Oryza sativa L.) Based on Relative Expression of Homogentisate Geranylgeranyl Transferase (HGGT) and Granule-Bound Starch Synthase I (GBSSI) Gene

2021 ◽  
Vol 28 (2) ◽  
pp. 181
Author(s):  
Dyati Galuh Pratita ◽  
Tita Putri Milasari ◽  
Elhah Nailul Khasna ◽  
Sunarmi Sunarmi ◽  
Evi Susanti ◽  
...  
Keyword(s):  
Glycemic Index ◽  
Amylose Content ◽  
Oryza Sativa L ◽  
Rice Varieties ◽  
Relative Expression ◽  
Qrt Pcr ◽  
Genetic Potential ◽  
Geranylgeranyl Transferase ◽  
High Amylose ◽  
Low Glycemic Index

Rice nutrition including vitamin and amylose contents become important aspect for many people around the world. Rice with high amylose content (low glycemic index) is good for those with Diabetes mellitus. Tocotrienol, one precursor of Vitamin-E biosynthesis is catalyzed by enzymes encoded HGGT, while amylose biosynthesis is catalyzed by enzymes encoded GBSSI. The objective of this study was to find rice varieties with high tocotrienol and/or amylose content based on the expression of HGGT and GBSSI among eight Banyuwangi local rice varieties. Relative expression of HGGT and GBSSI was measured by qRT-PCR and analyzed using 2ΔCt method. Statistical analysis resulted in the significantly different of HGGT and GBSSI relative expression among samples. Relative expression of HGGT from the highest to the lowest were demonstrated by Hitam Melik, Hitam Pekat, Blambangan A3, Merah Bali, Blambangan A2, Berlian, Janur Kuning, and SOJ A3, respectively; while relative expression of GBSSI from the highest to the lowest were demonstrated by Hitam Melik, Hitam Pekat, SOJ A3, Janur Kuning, Berlian, Merah Bali, Blambangan A3, and Blambangan A2, respectively. Based on this research we conclude that Hitam Melik potentially produces higher tocotrienol and lower glycemic index than other studied varieties.

scholarly journals Superior haplotypes towards development of low glycemic index rice with preferred grain and cooking quality

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ramchander Selvaraj ◽  
Arun Kumar Singh ◽  
Vikas Kumar Singh ◽  
Ragavendran Abbai ◽  
Sonali Vijay Habde ◽  
...  
Keyword(s):  
Glycemic Index ◽  
Resistant Starch ◽  
Amylose Content ◽  
Association Studies ◽  
Consumer Preference ◽  
Genome Wide Association Studies ◽  
Market Demand ◽  
Rice Varieties ◽  
Low Glycemic Index ◽  
Trait Associations

AbstractIncreasing trends in the occurrence of diabetes underline the need to develop low glycemic index (GI) rice with preferred grain quality. In the current study, a diverse set of 3 K sub-panel of rice consisting of 150 accessions was evaluated for resistant starch and predicted glycemic index, including nine other quality traits under transplanted situation. Significant variations were noticed among the accessions for the traits evaluated. Trait associations had shown that amylose content possess significant positive and negative association with resistant starch and predicted glycemic index. Genome-wide association studies with 500 K SNPs based on MLM model resulted in a total of 41 marker-trait associations (MTAs), which were further confirmed and validated with mrMLM multi-locus model. We have also determined the allelic effect of identified MTAs for 11 targeted traits and found favorable SNPs for 8 traits. A total of 11 genes were selected for haplo-pheno analysis to identify the superior haplotypes for the target traits where haplotypes ranges from 2 (Os10g0469000-GC) to 15 (Os06g18720-AC). Superior haplotypes for RS and PGI, the candidate gene Os06g11100 (H4-3.28% for high RS) and Os08g12590 (H13-62.52 as intermediate PGI). The identified superior donors possessing superior haplotype combinations may be utilized in Haplotype-based breeding to developing next-generation tailor-made high quality healthier rice varieties suiting consumer preference and market demand.

scholarly journals Glycemic index studies in rice (Oryza sativa L.) varieties developed by ANGRAU

2021 ◽  
Vol 17 (AAEBSSD) ◽  
pp. 133-137
Author(s):  
S. Divya Prasanna Kumari ◽  
G. Nirmala Devi ◽  
N. Chamundeswari
Keyword(s):  
Glycemic Index ◽  
Significant Variation ◽  
Agricultural Research ◽  
Amylose Content ◽  
Oryza Sativa L ◽  
Research Station ◽  
Rice Varieties ◽  
Gel Consistency ◽  
The Mean ◽  
Chemical Traits

Twenty popular rice varieties developed by ANGRAU obtained from Regional Agricultural Research Station (RARS), Maruteru during Kharif – 2019, were screened for chemical quality traits viz., gel consistency, gelatinization temperature, amylose along with glycemic index.In our study, significant variation was observed among all the varieties for chemical traits but no significant variation was observed in case of glycemic index.The amylose content observed over the varieties ranged from 21.7 per cent to 24.73 per cent. All the varieties have intermediate amylose content. Gel consistency ranged from 37.67 (hard gel) to 75 (soft gel) with the mean of 52.57. The alkali spreading value was noticed to be low, intermediate and high among all rice varieties.Glycemic index rangedfrom 56.72 -66.43 with a general mean of 60.67. Furthermore, all the varieties were categorized as medium GI varieties (56-69). Among all the varieties, Pushyami has shown the least value (56.72) for glycemic index.From the present investigation, it is concluded that, Rice varieties such as Chandra, Indra, Sri Dhruthi, Swarna, Samba Mahsuri, Sujatha, Maruteru Samba, MaruteruMahsuri recorded intermediate amylose, alkali spreading value and medium GI, which are desirable for rice consumers while counting for better rice with the best quality and glycemic index.

scholarly journals Karakteristik Fisik dan Kimia Beras Indigenous dari Lahan Pasang Surut di Kalimantan Tengah

JURNAL PANGAN ◽  
2018 ◽  
Vol 27 (2) ◽  
pp. 107-116
Author(s):  
Elmi Kamsiati
Keyword(s):  
Amylose Content ◽  
Chemical Properties ◽  
Chemical Characteristics ◽  
Rice Varieties ◽  
Central Kalimantan ◽  
Tidal Swamp ◽  
Production Area ◽  
High Amylose ◽  
Physical And Chemical ◽  
Low Glycemic Index

AbstrakBeras merupakan bahan pangan pokok penting bagi penduduk dunia. Lahan pasang surut memiliki potensi untuk menjadi daerah penghasil beras. Varietas padi/beras yang tumbuh dan beradaptasi dengan baik pada lahan pasang surut dapat dikembangkan pada program pemuliaan padi. Penelitian ini bertujuan untuk mengetahui karakteristik beras indigenous yang berasal dari lahan pasang surut di Kalimantan Tengah, terkait dengan sifat fisik dan kimianya. Ada delapan varietas beras yang dikarakterisasi yaitu Karang Dukuh, Siam Jurut, Siam Pandak, Siam Palun, Siam Palas, Bayar Pahit, Rantul dan Siam Unus. Sifat fisik yang dianalisis adalah dimensi, kekerasan dan derajat putih. Sedangkan sifat kimia yang diamati adalah kadar amilosa, lemak dan kadar air. Hasil penelitian menunjukkan bahwa varietas Siam Palas, Siam Pandak, Bayar Pahit, Rantul merupakan beras dengan bentuk medium, sedangkan empat lainnya merupakan beras dengan bentuk ramping (slender). Nilai derajat putih beras berkisar antara 45.3-50.9 yang setara dengan derajat sosoh 83-93%. Beras tersebut memiliki kadar amilosa berkisar antara 26.23-29.17%, yang termasuk beras dengan kadar amilosa tinggi. Sedangkan kadar lemaknya cukup rendah, berkisar antara 0,32-0,62%. Dari penelitian ini diketahui bahwa beras dari lahan pasang surut memiliki kadar amilosa yang tinggi yang memiliki potensi indeks glikemik yang rendah.  AbstractRice is one of important staple food of world’s population. Tidal swamp land have potential resouces to be rice production area. Rice varieties that grows and well adapted in tidal swamp land can be developed in breeding program. The aims of this study was to investigate the physical and chemical characteristics of indigenous rice from tidal swamp land in Central Kalimantan. Paddy got from paddy breeder in Central Kalimantan. There are eight indigenous varieties of rice which is characterized namely Siam Palas, Siam Jurut, Siam Pandak, Siam Palun, Bayar Pahit, Rantul, Karang Dukuh and Siam Unus. The physical properties that observed were dimensions, hardness and whiteness. Amylose, fat and moisture content were the chemical properties that analyses. The result showed that based on shape, Siam Palas, Siam Pandak, Bayar Pahit, Rantul varieties has moderate shape, and the others has slender shape. The whiteness degree of rice ranged between 45.3-50.9 which is equivalent to 83-93% milling degree. The rice had high amylose content ranged between 26.23-29.17. While the fat contents fairly low, ranged between 0.32-0.62%. From this research, rice from tidal swamp land has high amylose content that potential as rice with low glycemic index.  

scholarly journals Evaluation of Starch Hydrolysis for Glycemic Index Prediction of Rice Varieties

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 101
Author(s):  
Cristiana Pereira ◽  
Regina Menezes ◽  
Vanda Lourenço ◽  
Teresa Serra ◽  
Carla Brites
Keyword(s):  
Glycemic Index ◽  
Food Processing ◽  
Amylose Content ◽  
Rice Variety ◽  
Great Influence ◽  
Starch Hydrolysis ◽  
Rice Starch ◽  
Rice Varieties ◽  
Processing Technologies

Rice consumed as white cooked polished grain has been considered a high glycemic index (GI) food, particularly compared with other starchy foods. However, the GI levels of rice based food can vary among different rice types and food processing technologies. Rice GI variation can be affected by several factors, such as rice variety, the genetic background of rice as well as due to crop edaphoclimatic conditions. The main difference in rice starch composition that influences GI is the amylose content. Besides the chemical composition of rice, the gelatinization characteristics and food processing can also contribute to starch retrogradation, thus increasing the level of resistant starch with a great influence on GI. To understand the glycemic response of rice types differing in amylose and viscosity profiles, four rice samples were analyzed and compared with standard and resistant HI-MAIZE corn starches. An in vitro enzymatic starch hydrolysis procedure was applied to estimate GI. The results indicate substantial differences in the starch hydrolysis of the two corn starches. Starch hydrolysis tended to be more rapid and efficient for ‘Waxy’ and ‘Ceres’ (intermediate-amylose) rice types than for ‘Maçarico’ (high-amylose rice). In addition, the data show that the Maçarico variety has the lowest estimated GI and the highest retrogradation rate compared with ‘Waxy’, ‘Ceres’ and ‘Basmati’ type. The results obtained reinforce the importance of knowing amylose content and viscosity profiles for the prediction of rice glycemic responses.

Melting the secrets of gelatinisation temperature in rice

2010 ◽  
Vol 37 (5) ◽  
pp. 439 ◽  
Author(s):  
Rosa P. Cuevas ◽  
Venea D. Daygon ◽  
Henry M. Corpuz ◽  
Leilani Nora ◽  
Russell F. Reinke ◽  
...  
Keyword(s):  
Amylose Content ◽  
Oryza Sativa L ◽  
Length Distribution ◽  
Cooking Time ◽  
Scanning Calorimetry ◽  
Rice Varieties ◽  
Chain Length Distribution ◽  
Chain Lengths ◽  
Major Grouping ◽  
Upper Boundary

Gelatinisation temperature (GT) is one of the key traits measured in programs for breeding rice (Oryza sativa L.). It is commonly estimated by the alkali spreading value (ASV), and less commonly by differential scanning calorimetry (DSC). Using a diverse set of germplasm, it was determined that DSC values associate poorly with ASV, are not correlated with amylose content but correlate with cooking time. Rice varieties are traditionally grouped into three classes of GT based on ASV: high, intermediate and low. However, the distribution of DSC values of 4000 samples shows only two classes: high and low. Large differences in the distributions of chain lengths synthesised by starch synthase IIa (SSIIa) support the two classes as the major grouping, two haplotypes associating with each peak. Each peak of DSC values spanned 10°C. The chain length distribution of the amylopectin molecules from varieties at the upper boundary of each peak showed significantly more chains that span both the crystalline and amorphous lamellae of a cluster than varieties at the other end of that distribution. Improved varieties, classified as intermediate GT by ASV, belong to both of the classes defined by DSC, implying that some enzyme, other than SSIIa is involved in intermediate GT.

scholarly journals Texture and Digestion Properties of Cooked Rice and Rice Noodles

2021 ◽  
Author(s):  
Liqin Hu ◽  
Zhengwu Xiao ◽  
Jiana Chen ◽  
Jialin Cao ◽  
Anas Iqbal ◽  
...  
Keyword(s):  
Amylose Content ◽  
Glucose Production ◽  
Pasting Properties ◽  
Starch Digestion ◽  
Rice Varieties ◽  
Cooked Rice ◽  
Apparent Amylose Content ◽  
High Amylose ◽  
Insulin Responses

Abstract Rice varieties high in amylose content have low glycemic and insulin responses. Rice noodles are processed by extrusion from high amylose content rice, which may also have low glycemic and insulin responses. In this study, cooked rice and rice noodles processed from two high amylose content cultivars, Guangluai4 (GL4) and Zhenguiai (ZGA), were chosen for in vitro starch digestion evaluation. Apparent amylose content of cultivars (i.e., GL4-28.4% and ZGA-26.8%) and pasting properties except final viscosity were significantly different between the cultivars. In vitro starch digestion results showed that the glucose production rate in rice noodles was significantly slower than that in cooked rice by 65.7% and 42.0% in GL4 and ZGA, respectively. The main reason for low glucose production in rice noodles was active digestion duration longer in rice noodles than in cooked rice, which reflects the slow release of glucose during starch digestion. The texture of rice noodles in the GL4 and ZGA cultivars is 3 and 2.3 times harder than that of cooked rice, respectively. Thus digestive enzymes can hardly enter the interior of rice noodles for amylolysis. As a result, the digestion time of rice noodles is longer, and the release of glucose during digestion is slower than that of cooked rice. The slower release of glucose during rice noodle digestion may be beneficial for prolonging satiety and reducing food intake. Consequently, eating rice noodles may help in improving or preventing diabetes and obesity over time.

scholarly journals Estimated glycemic index of rice increases with the release of new varieties in the middle reach of the Yangtze River

Discover Food ◽  
2022 ◽  
Vol 2 (1) ◽  
Author(s):  
Min Huang ◽  
Zhengwu Xiao ◽  
Liqin Hu ◽  
Jiana Chen ◽  
Fangbo Cao
Keyword(s):  
Glycemic Index ◽  
Yangtze River ◽  
Amylose Content ◽  
The Yangtze River ◽  
Middle Reach ◽  
Rice Varieties ◽  
Potential Health ◽  
Estimated Glycemic Index ◽  
Increasing Trend ◽  
New Varieties

AbstractThe amylose content of newly-released rice varieties has steadily decreased in rice-growing regions in the middle reach of the Yangtze River during 2006–2021, to parallel increasing consumption of soft-textured rice with low amylose content. We estimated the glycemic index of these newly-released rice varieties and reveal a significantly increasing trend, showing a 0.3 annual increase in estimated glycemic index of new rice varieties released each year. These results highlight the need to assess the potential health risks associated with the development of soft-textured rice with low amylose rice.

scholarly journals FAKTOR YANG MEMPENGARUHI INDEKS GLIKEMIK RENDAH PADA BERAS DAN POTENSI PENGEMBANGANNYA DI INDONESIA / Factors Affecting the Low Glycemic Index on Rice and Its Potential for Development in Indonesia

2019 ◽  
Vol 38 (2) ◽  
pp. 105
Author(s):  
Siti Dewi Indrasari
Keyword(s):  
Development Strategy ◽  
Glycemic Index ◽  
Dietary Habits ◽  
Rice Variety ◽  
Added Value ◽  
Rice Varieties ◽  
Certification Process ◽  
Factors Affecting ◽  
Low Glycemic Index ◽  
Diabetes Melitus

<p>Diabetes Mellitus (DM) is a disorder of glucose metabolism due to insulin deficiency both in absolute and relative terms caused by changes in dietary habits that result in obesity. Nationally in Indonesia, the prevalence of DM based on a doctor’s diagnosis in the population aged over or equal to 15 years is 2%. Consumption of rice with a low glycemic index is one way to regulate dietary patterns for people with type 2 diabetes. In Indonesia, rice varieties that have a low glycemic index (GI) value have been successfully identified. This paper aims to provide information and understanding of GI rice, influencing factors and strategies for developing rice with low GI. Some rice varieties that have low GI are include IR 36, Logawa, Batang Lembang, Ciherang, Cisokan, Margasari, Martapura, Air Tenggulang, Hipa 7, Inpari 12, Inpari 13, Situ Patenggang, Pandanwangi, Inpari 1, Beras Hitam Subang, Inpara 4. Factors affecting rice GI include rice varieties and amylose-amylopectin ratio, processing method, protein and fat, dietary fiber content and starch digestibility. The target of developing rice with a low GI is a community with a high prevalence of DM. While the development strategy is to strive so that rice varieties with a low GI that are beneficial to health can be regulated in the Ministry of Agriculture Regulation as part of special rice. Rice varieties with these advantages can be developed through the process of releasing varieties, followed by the certification process for Labelled Rice Variety Assurances (LRVA). The certification process aims to provide functional rice that has added value, has a high selling power for farmers and producers and guarantees the rights of consumers who consume it.</p><p>Keywords: Rice. glycemic index, diabetes melitus </p><p> </p><p><strong>Abstrak</strong></p><p> Penyakit diabetes melitus (DM) merupakan gangguan metabolisme glukosa akibat kekurangan insulin, baik secara absolut maupun relatif yang disebabkan oleh perubahan kebiasaan pola makan yang mengakibatkan obesitas. Di Indonesia, prevalensi DM berdasarkan diagnosis dokter pada penduduk umur lebih dari atau sama dengan 15 tahun rata-rata 2%. Konsumsi beras dengan indeks glikemik rendah merupakan salah satu cara mengatur pola diet bagi para diabetesi tipe 2. Di Indonesia, varietas padi yang mempunyai nilai IG rendah telah berhasil diidentifikasi. Tulisan ini bertujuan untuk memberikan informasi dan pemahaman tentang IG beras, faktorfaktor yang mempengaruhi dan strategi untuk mengembangkan beras dengan IG rendah. Beberapa varietas padi yang mempunyai IG rendah antara lain IR36, Logawa, Batang Lembang, Ciherang, Cisokan, Margasari, Martapura, Air Tenggulang, Hipa-7, Inpari- 12, Inpari-13, Situ Patenggang, Pandanwangi, Inpari-1, Beras Hitam Subang, Inpara-4. Faktor yang mempengaruhi IG beras antara lain varietas padi dan rasio amilosa-amilopektin, cara pengolahan, protein dan lemak, kadar serat pangan dan daya cerna pati. Sasaran pengembangan beras dengan IG rendah adalah masyarakat dengan prevalensi DM yang tinggi. Strategi pengembangannya adalah mengupayakan agar varietas padi dengan IG rendah yang bermanfaat untuk kesehatan dapat diatur dalam Permentan sebagai bagian dari beras khusus. Varietas padi dengan keunggulan tersebut dapat dikembangkan melalui proses pemutihan atau pelepasan varietas yang dilanjutkan dengan proses sertifikasi Beras Berlabel Jaminan Varietas (BBJV). Proses sertifikasi ini bertujuan agar beras-beras fungsional tersebut memperoleh nilai tambah, berdaya jual tinggi bagi petani dan menjamin hak konsumen yang mengonsumsi.</p><p>Kata kunci: Beras, indeks glikemik, diabetes melitus </p>

scholarly journals Cooking and functional properties of parboiled milled local rice marketed in the south-east zone of Nigeria

2020 ◽  
Vol 9 (2) ◽  
pp. 319-331
Author(s):  
Chinenye E. Azuka ◽  
Iro Nkama ◽  
Chinwendu R. Eze ◽  
Nahemiah Danbaba ◽  
Felix U. Asoiro
Keyword(s):  
Functional Properties ◽  
Amylose Content ◽  
Expansion Ratio ◽  
Grain Weight ◽  
Elongation Ratio ◽  
Rice Varieties ◽  
Cooking Properties ◽  
Gel Consistency ◽  
High Amylose ◽  
Locally Grown

Imported rice is perceived to have better cooking properties than locally grown rice in Nigeria and it has increased its market share while reducing patronage for local rice. Rice in Nigeria has many applications, including consumption as whole cooked grain or dumpling or use as an adjunct in making beverages. Eighteen varieties of parboiled milled local rice and three imported rice varieties, coded Ip1, Ip2 and Ip3, were studied for their cooking and functional properties using standard methods. There was a significant (p<0.05) increase in the dimensions of all the rice varieties when cooked. There was a 25 g increase in the grain weight and an elongation ratio of more than 1.26 in all the rice varieties. Ghesua had the highest cooked grain weight (68.67 g) while Omor-Mas (6.00) and R-Bus (6.00) had the highest volume expansion ratio (VER). The VER was more than 3.00 for all the rice varieties. All the local rice varieties imbibed less water (17.67-25.33 ml) compared to the imported rice varieties (26.00-27.67ml) before they reached their optimum cooking time. The imported rice varieties were of soft gel consistency (89.67-73.50 mm) and intermediate amylose content (20.71-23.14 %) while the local rice varied in amylose and gel consistency. Abakiliki-Mas (27.00 mm) and R-8 (33.67 mm) were of hard gel-consistency, intermediate (21.11 %) and high amylose (27.21 %) content respectively and have not been exploited although they would be appropriate for making canned rice, dry mixes and rice-noodles.

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