scholarly journals Evaluation de l’activité antipyrétique, analgésique et anti-inflammatoire de l’extrait méthanolique de Vitex congolensis De Wild. & T. Durand

2020 ◽  
Vol 13 (7) ◽  
pp. 3066-3078
Author(s):  
Victor Okombe Embeya ◽  
Gaël Nzuzi Mavungu
Keyword(s):  
Guinea Pigs ◽  
Methanolic Extract ◽  
Democratic Republic Of Congo ◽  
Physiological Saline ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Root Bark ◽  
Distilled Water ◽  
Anti Inflammatory

En République Démocratique du Congo, Vitex congolensis De Wild. & T. Durand (Lamiaceae) est une plante médicinale largement utilisée dans le traitement des pathologies à composante inflammatoire. La présente étude a eu pour objectif d’évaluer les activités antipyrétique, analgésique et anti-inflammatoire de l’extrait méthanolique de la poudre d’écorce de racine de cette espèce sur des modèles de pyrexie, algésie et  inflammation induites chez les cobayes. L’étude a concerné 24 cobayes de deux sexes répartis en quatre lots de 6 animaux : un lot témoin positif traité avec du diclofenac en intrapéritonéale, un lot témoin négatif ayant reçu soit de l’eau distillée per os soit du sérum physiologique en  intrapéritonéale ainsi que deux lots ayant respectivement reçu 100 mg et 300 mg de l’extrait méthanolique de la poudre d’écorce de racines de V. congolensis par kg de poids corporel par voie orale. Les deux doses (100 et 300 mg) de l’extrait méthanolique préviennent de façon significative, le processus inflammatoire comparativement aux lots standard (médicaments de références) et contrôle négatif (eau distillée/sérum physiologique). L’étude a montré que l’extrait méthanolique d’écorces de racine de V. congolensis pourrait être une source potentielle des nouveaux  médicaments anti-inflammatoires.Mots clés: antipyrétique, analgésique, anti-inflammatoire, extrait  méthanolique, Vitex congolensis De Wild. & T. Durand. English Title: Antipyretic, analgesic and anti-inflammatory activity of the methanolic extract of Vitex congolensis De Wild. & T. DurandIn the Democratic Republic of Congo, Vitex congolensis De Wild. & T. Durand (Lamiaceae) is a medicinal plant widely used in the treatment of pathologies with an inflammatory component. The main objective of this study was to evaluate the antipyretic, analgesic and anti-inflammatory activities of the methanolic extract of the powder of this species on models of pyrexia, algesia and inflammation induced in guinea pigs. The study concerned 24 guinea pigs of both sexes divided into 4 lots of six animals each : a positive control group treated with diclofenac intraperitoneally, a negative control group that received either oral or intraperitoneal (distilled water / physiological saline) ; and two groups respectively receiving 100 mg and 300 mg of the methanolic extract of the root bark powder of V. congolensis per kg of body weight orally. The two doses (100 and 300 mg) of the methanolic extract significantly prevent the inflammatory process compared to standard batches (reference drugs) and negative control (distilled water / physiological saline). The study showed that the methanolic extract of V. congolensis root bark could be a potential source of new anti-inflammatory drugs.Keywords: antipyretic; analgesic, anti-inflammatory, methanolic crude, Vitex congolensis De Wild. & T.Durand.

scholarly journals ASSESSMENT OF ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC EXTRACT OF ASAM KANDIS (GARCINIA XANTHOCHYMUS HOOK. F. EX T. ANDERSON) FRUIT

2018 ◽  
Vol 11 (4) ◽  
pp. 81
Author(s):  
Hanafis Sastra Winata ◽  
Rosidah Rosidah ◽  
Panal Sitorus
Keyword(s):  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Control Group ◽  
Phytochemical Screening ◽  
Fruit Extract ◽  
Garcinia Xanthochymus ◽  
Anti Inflammatory

 Objective: The objective of this study was to evaluate the anti-inflammatory activity in acute and subacute models of inflammation from ethanolic fruit extract of Asam kandis (Garcinia xanthochymus Hook. f. ex T. Anderson) in animal (rats) models.Methods: Pleliminary phytochemical screening was carried out by using standard procedures.. Assessment of acute and subacute models of inflammation was using carrageenan-induced paw edema method and cotton pellet granuloma method using three dosage treatments; 200 mg/kg BW, 400 mg/kg BW, and 800 mg/kg BW along with a negative control group (0.5% Na CMC) and positive control (Na diclofenac 2.25 mg/kg BW). The inhibition period was observed at 30, 60, 90, 120, 150, and 180 min time intervals.Result: The phytochemical screening showed that the ethanolic fruit extract from Asam kandis contain contains flavonoids, glycosides, steroids, and triterpenoids. The anti-inflammatory result showed that the strongest inhibition produced by ethanolic fruit extract of Asam kandis occurred on the dosage of 800 mg/kg BW compared to the other doses (200 and 400 mg/kg BW) throughout the observation period.Conclusion: This finding indicated that ethanolic fruit extract of Asam kandis (G. xanthochymus Hook. f. ex T. Anderson) might become an interesting candidate for treatment of inflammation.

scholarly journals Silver Nanoparticle of Acalypha indica Linn. Leaf As Bio-larvicide against Anopheles sp. Larvae

2021 ◽  
Vol 9 (A) ◽  
pp. 760-765
Author(s):  
Yos Banne ◽  
Olfie Sahelangi ◽  
Steven Soenjono ◽  
Elisabeth Natalia Barung ◽  
Selfie Ulaen ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Group ◽  
Distilled Water ◽  
Lc50 Value ◽  
Acalypha Indica

BACKGROUND: Acalypha indica Linn. has been used as traditional medicine, it contains flavonoids, alkaloids, tannins, saponins, steroids, triterpenoids, and essential oils. AIM: This study aimed to determine the bio-larvicide effects of A. indica Linn. leaf stew and the silver nanoparticles against Anopheles sp. larvae. METHODS: The fresh leaves of A. indica Linn. extracted using distilled water at 100°C for 30 min. The silver nanoparticles were made by mixing a solution of silver nitrate with the stew, which acts as a reducing agent. The resulting silver nanoparticles were characterized by particle size analyzer and UV-vis spectrophotometer. The bio-larvicide effects against Anopheles sp. larvae performed using a completely randomized design. There were eight groups consisted of ten larvae and three replications. Treatment groups of stew and silver nanoparticle for concentrations 0.05%, 0.5%, and 5%, respectively. The negative control group was distilled water and the positive control group was the 0.01% abate solution. Assessment of larvicide activity was carried out every hour for 6 h and continued if there were larvae that live up to 24 h. The LC50 value was calculated based on Probit analysis. RESULTS: The results showed that the A. indica Linn. leaf stew can be made into silver nanoparticles preparations, optimal results were obtained from a mixture of 1% stew and 3 mM AgNO3. The result of bio-larvicides effect test against Anopheles sp. larvae showed that the LC50 value of the A. indica Linn. leaf stew was 727,3 ppm and the LC50 value of silver nanoparticles was 3.366 ppm. CONCLUSION: It can be concluded that A. indica Linn. is a promising larvicidal plant and can be made into silver nanoparticle preparations.

scholarly journals Potensi Minyak Sawit Merah Dan Karotenoid Sebagai Suplemen Antioksidan Dalam Pengujian Toleransi Glukosa Pada Tikus Putih (Preliminary Study)

2018 ◽  
Vol 1 (1) ◽  
pp. 251-256 ◽  
Author(s):  
Ahmad Gazali Sofwan Sinaga ◽  
Donald Siahaan ◽  
Kasmirul Ramlan Sinaga
Keyword(s):  
Blood Glucose ◽  
Palm Oil ◽  
Preliminary Test ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Distilled Water ◽  
Red Palm Oil ◽  
Dpph Method ◽  
Anti Oxidant

Minyak sawit merah (MSM) adalah salah satu produk olahan dari minyak kelapa sawit, dan merupakan sumber makanan yang kaya karotenoid sekitar 500 - 800 ppm. Konsentrat karotenoid (KK) juga merupakan produk yang diperoleh dari proses penghilangan minyak sehingga kadar karotenoid meningkat hingga 6000 - 7000 ppm. Penelitian ini bertujuan untuk mengetahui potensi penggunaan minyak sawit merah dan konsentrat karotenoid sebagai uji pendahuluan suplemen antioksidan dalam pengujian toleransi glukosa pada tikus putih. Pengujian aktivitas antioksidan dari minyak sawit merah dan konsentrat karotenoid diuji dengan metode DPPH dengan masa inkubasi selama 60 menit. Pengujian efektivitas antihiperglikemia dilakukan menggunakan hewan percobaan (n=40) yang dibagi menjadi 8 grup: grup kontrol negatif (akuades), kontrol positif (glibenklamid 10 mg), tiga grup diberi minyak sawit merah dengan dosis 1, 2, dan 3 ml, dan tiga grup diberi konsentrat karotenoid dengan dosis 1, 2, dan 3 ml. Penurunan kadar glukosa darah selama 180 menit pada uji antihiperglikemia sebagai berikut glibenklamid 10 mg> MSM 3 ml> MSM 2 ml> MSM 1 ml> KK 3 ml> KK 2 ml> KK 1 ml dan akuades. Pemberian minyak sawit merah dapat menurunkan kadar glukosa darah lebih cepat dibandingkan konsentrat karotenoid. Sifat antioksidatif komponen minor minyak sawit merah dapat menghambat oksidasi glukosa di dalam darah, sehingga pankreas dapat aktif memproduksi insulin secara normal. Oleh karena itu, minyak sawit merah dapat digunakan secara sinergis untuk membantu kerja obat hiperglikemia oral (OHO). Red Palm Oil is one of processed products from palm oil and also a food source rich in carotenoid,around500-800 ppm. Carotenoid concentrate is also a product obtained from the oil removal process and the carotenoid level increases to 6000-7000 ppm. This research aimed to determinethe potential of the red palm oil and the carotenoid concentrate as a preliminary test of anti-oxidant supplement Glucose Tolerance Test on Rat. The anti-oxidant activity of red palm oil and carotenoid concentrate was tested using DPPH method with 60 minutes incubation period. The anti-hyperglycemia activity evaluationwas conducted using animals(n=40) which divided into 8 groups: Negative control group (distilled water), positive control (glibenclamide 10 mg), three groups were given red palm oil with dose of1, 2, and 3 ml , and three groups were given carotenoid concentrate with dose of 1, 2, and 3 ml . The decrease of blood glucose for 180 minutes in anti-hyperglycemia test were in order ofglibenclamide10 mg> MSM 3 ml> MSM 2 ml> MSM 1 ml> KK 3 ml> KK 2 ml> KK 1 ml and distilled water. The red palm oil induction could lower blood glucose level faster than carotenoid concentrate, The anti-oxidative properties of minor componentsin red palm oil prevent the blood glucose oxidation and the pancreas could be actively produce insulin normally. In conclusion, red palm oil could be used synergistically toenhance the effectiveness oforal hyperglycemic agent.

scholarly journals Immunomodulatory and Anti-Inflammatory Potentials of Trigona Honey in the Therapy and Prevention against Respiratory Infection in Wistar Rats

2020 ◽  
Vol 11 (3) ◽  
pp. 2955-2962
Author(s):  
Ibrahim Khaled Al-kafaween ◽  
Abu Bakar Mohd Hilmi ◽  
Mohamed M. Soliman
Keyword(s):  
Respiratory Infection ◽  
Wistar Rats ◽  
Serum Levels ◽  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Gene Expressions ◽  
Anti Inflammatory

Trigona honey (TH) is well known for its therapeutic characteristics. To date, the study of Trigona honey as a prophylactic or immune booster prior to the bacterial infection of the invivo model is not well covered. This study aims to investigate anti-inflammatory and immune activities in Wistar rats infected with respiratory infection following with Trigona honey. 25 Wistar rats were assigned to possitive groups, negative control group, positive control group was fed TH (5 g / kg body weight) orally, the untreated group was infected with Staphylococcus aureus to induce respiratory infection, the treated group has been infected with S. aureus followed by treatment with TH at a dose of 1.5 ×108 CFU / mL and the preventive group ingested TH one week before S. aureus infection. Blood was obtained for biochemical analysis. Lung tissues have been collected for molecular examination. The results showed a significant decrease in serum levels of ALT, AST, urea and creatinine in the preventive and treated groups, serum IgG increased significantly (P<0.05) in the preventive and treated groups, IFN-y increased in the preventive group while decreased in the treated group, and IL-8 increased in the treated group while decreased in the preventive group. The mRNA expression of AGP is up-regulated in the positive control, preventive and treated groups. The α2-MG, TNF-α , and mRNA expressions showed lower regulation after administration of TH in preventive and treated groups. The results show the ability of TH to counteract immune and inflammatory changes in serum levels and gene expressions.

scholarly journals UJI EFEK ANTIPIRETIK EKSTRAK MENIRAN (Phyllantus niruri L.) PADA TIKUS WISTAR (Rattus norvegicus) JANTAN YANG DIINDUKSI VAKSIN DPT-HB

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Ivana Jansen ◽  
Jane Wuisan ◽  
Henoch Awaloei
Keyword(s):  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Distilled Water ◽  
Phyllanthus Niruri ◽  
Antipyretic Effect ◽  
Flower Extract ◽  
Hb Vaccine

Abstract: The aim of this study is to investigate the antipyretic effect of leaf-flower extract (Phyllanthus niruri L.) in Wistar rats induced by DPT-HB vaccine. Samples were 15 Wistar rats as test animals and were divided into five groups of three in each. The negative control group was given orally distilled water, positive control group was given paracetamol and 3 experimental groups were given ethanolic leaf-flower extracts (100 mg, 200 mg and 300 mg /200 g body weight of rats). Pyrexia was induced in rats by intramuscular injection of 0.2 ml DPT-HB vaccine. Antipyretic activity was measured at 0, 30, 60, 90, 120, 150, 180 minutes after administration of leaf-flower extract, paracetamol and distilled water. The results showed that leaf-flower extract at a dose of 300 mg /200 g BW decreased the rectal temperature greater than with doses of 100 and 200 mg /200 g BW for 180 minutes of measurement. Conclusion: Leaf-flower extract has antipyretic effect in Wistar rats.Keywords: antipyretic, phyllanthus niruri, DPT-HB, wistar ratAbstrak: Penelitian ini bertujuan untuk menguji efek antipiretik ekstrak meniran (Phyllanthus niruri L.) pada tikus Wistar yang diinduksi vaksin DPT-HB. Metode: Hewan uji yang digunakan adalah 15 ekor tikus Wistar yang dibagi ke dalam 5 kelompok, masing-masing kelompok terdiri dari 3 ekor tikus. Kelompok kontrol negatif diberikan aquades, kelompok kontrol positif diberikan parasetamol, dan 3 kelompok eksperimental diberikan ekstrak tanaman meniran masing-masing dengan dosis 100mg, 200mg dan 300 mg/200 grBB tikus. Induksi demam pada hewan uji menggunakan vaksin DPT-HB 0,2ml secara intramuskular. Pengukuran suhu rektal dilakukan sebelum dan sesudah pemberian vaksin dan setelah pemberian bahan uji yaitu pada menit ke-30, 60, 90, 120, 150 dan 180. Hasil penelitian memperlihatkan pemberian ekstrak meniran dengan dosis 300 mg/200 grBB menunjukkan penurunan suhu rektal lebih besar dibanding dengan dosis 100 dan 200 mg/200 grBB selama 180 menit pengukuran. Simpulan: Ekstrak meniran memiliki efek antipiretik pada tikus Wistar.Kata kunci: antipiretik, phyllanthus niruri, DPT-HB, tikus wistar

scholarly journals Regulation of Inflammatory Response in Human Osteoarthritic Chondrocytes by Novel Herbal Small Molecules

2019 ◽  
Vol 20 (22) ◽  
pp. 5745 ◽  
Author(s):  
Ziadlou ◽  
Barbero ◽  
Stoddart ◽  
Wirth ◽  
Li ◽  
...  
Keyword(s):  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Catabolic Genes ◽  
Osteoarthritic Chondrocytes ◽  
Culture Model ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Safranin O ◽  
Vanilic Acid

In this study, 34 Traditional Chinese Medicine (TCM) compounds were screened for potential anabolic and anti-inflammatory properties on human osteoarthritic (OA) chondrocytes. The anabolic effects were assessed by measuring the glycosaminoglycan (GAG) relative to the DNA content using a 3D pellet culture model. The most chondrogenic compounds were tested in an inflammatory model consisting of 3 days of treatment with cytokines (IL-1β/TNF-α) with or without supplementation of TCM compounds. The anti-inflammatory effects were assessed transcriptionally, biochemically and histologically. From the 34 compounds, Vanilic acid (VA), Epimedin A (Epi A) and C (Epi C), 2′′-O-rhamnosylicariside II (2-O-rhs II), Icariin, Psoralidin (PS), Protocatechuicaldehyde (PCA), 4-Hydroxybenzoic acid (4-HBA) and 5-Hydroxymethylfurfural (5-HMF) showed the most profound anabolic effects. After induction of inflammation, pro-inflammatory and catabolic genes were upregulated, and GAG/DNA was decreased. VA, Epi C, PS, PCA, 4-HBA and 5-HMF exhibited anti-catabolic and anti-inflammatory effects and prevented the up-regulation of pro-inflammatory markers including metalloproteinases and cyclooxygenase 2. After two weeks of treatment with TCM compounds, the GAG/DNA ratio was restored compared with the negative control group. Immunohistochemistry and Safranin-O staining confirmed superior amounts of cartilaginous matrix in treated pellets. In conclusion, VA, Epi C, PS, PCA, 4-HBA and 5-HMF showed promising anabolic and anti-inflammatory effects.

Evaluation of in-feed larch sawdust anti-inflammatory effect in sows

2017 ◽  
Vol 20 (2) ◽  
pp. 321-327
Author(s):  
E.D. Tzika ◽  
P.D. Tassis ◽  
V.G. Papatsiros ◽  
E.M. Pferschy-Wenzig ◽  
A. Siochu ◽  
...  
Keyword(s):  
Corn Starch ◽  
Post Partum ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Lactation Period ◽  
Basic Diet ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Abstract The study aimed to investigate the possible anti-inflammatory activity of larch sawdust as feed supplement in lactating sows’ diet and its possible effect on the prevalence of Postpartum Dysgalactia Syndrome under field conditions. In a Greek farrow-to-finish pig farm, fifteen sows were randomly and equally allocated to a negative control group (NC group), a positive control group (PC group), and a treatment group (LT group). The animals of the first two groups received 99% basic diet and 1% corn starch, while LT group animals received 99% basic diet and 1% larch sawdust. The whole trial period lasted 35 days (7 days prior to farrow – day of weaning). At parturition day, animals of the PC group received 2 ml of an anti-inflammatory drug intramuscularly (meloxicam, Metacam®, Boehringer Ingelheim Vetmedica), while the animals of both other groups, received 2 ml of normal saline. Results showed insignificant differences among experimental groups for parameters such as post-partum rectal temperature and piglets performance. On the contrary, a significant increase of mean milk lactation index was observed in LT and PC groups on the 4th day of lactation period, when compared with NC group (p=0.014). Additionally, mean IL-6 concentrations in blood in the LT group showed a tendency for reduction when compared with those found in NC, and insignificant difference (p>0.05) when compared with those observed in PC group 24 hours postpartum. Moreover, the respective TNFα mean level in the LT group at 24 and 72 hours after parturition was similar to that found in PC group, respectively) and significantly lower than that determined in the NC group (p=0.003, p=0.024. The results suggest a possible anti-inflammatory effect of larch sawdust in sows.

scholarly journals Effect of Long Bean (Vignaunguiculata) on Blood Glucose Level of Mice (Mus musculus) Induced by Alloxan

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Intan Fitri Aprila ◽  
M. Nur Salim ◽  
Razali Daud ◽  
T. Armansyah ◽  
Nuzul Asmilia ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Mus Musculus ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Distilled Water ◽  
Decrease Blood Glucose

The aim of this research was to find out the effect of long bean (Vigna unguiculata) on level of blood glucose on mice (Mus musculus) induced by alloxan. Twelve mice with the weigh of 25-40 grams and clinically healthy were used in this research. All mice were randomly divided into 4 treatment groups, 3 mice each. K0 was negative control group, only given aquadest. K1, K2, and K3 groups were induced by alloxan 0.5 ml. K1 group was positive control. K2 group was fed with 100 grams long bean mixed with 50 ml distilled water. K3 group was fed with 100 grams long bean mixed with 100 ml distilled water. Long bean was fed orally 0.5 ml every morning and evening for 7 consecutive days. On day eight the examination of blood glucose level was performed. The average level of blood glucose were K0 (142.00±23.39), K1 (167.00±10.54), K2 (122.67±12.50), dan K3 (154.67±16.26) mg/dL. In conclusion, the administration of long bean for seven consecutive days does not decrease blood glucose level on mice induced by alloxan.Key words: blood glucose, alloxan, long bean

scholarly journals Anti-Oxidant and Hepatoprotective Effects of Senecio biafrae on CCl4-induced Liver Damage in Rats

2019 ◽  
Vol 13 (2) ◽  
pp. 31-35
Author(s):  
Israel Oghenevwodoko Okoro ◽  
◽  
Helen Ejiro Kadiri ◽  
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Albino Rats ◽  
Root Extract ◽  
Distilled Water ◽  
Standard Drug ◽  
Anti Oxidant

Background: The present study was performed to explore whether the aqueous extract of Senecio biafrae (S. biafrae) roots provide any in vivo protective activity against carbon tetrachloride (CCl4)-induced hepatotoxicity in male albino rats. Methods: Rats (150-200 grams) were grouped into five groups (A-E) of six rats each and were treated orally for twelve days with 72 hourly administration of CCl4 (1 mL/kg) as follows: Group A received distilled water only (negative control), Group B was administered distilled water plus CCl4 (positive control), Group C was administered 400 mg/kg extract and CCl4, Group D received 200 mg/extract and CCl4, while Group E was administered standard drug (Silymarin 25mg/kg, PO). Results: Pre-treatment with the extract of S. biafrae (200 or 400mg/kg) or Silymarin (25mg/kg) caused significant restoration in the biomarkers as evaluated by reducing the levels of malondialdehyde, transaminases and elevating the levels of superoxide dismutase, catalase and glutathione peroxidase activities, which were altered by CCl4 toxicity. The extract at a dose of 400mg/kg demonstrated similar activities comparable to the standard drug (Silymarin). Conclusion: The results of this study indicate that the root extract of S. biafrae possesses hepatoprotective and anti-oxidant properties which may be due to the presence of phytochemicals in it.

scholarly journals Ekstrak Metanol Biji Asam Jawa (Tamarindus Indica) Memperbaiki Kerusakan Histopatologi Ginjal Tikus yang Diinduksi Aluminium

2020 ◽  
Vol 9 (2) ◽  
pp. 180
Author(s):  
Anang Dwi Atmoko ◽  
Al Munawir ◽  
Ika Rahmawati Sutejo
Keyword(s):  
Treatment Group ◽  
Methanolic Extract ◽  
Rat Kidney ◽  
Negative Control Group ◽  
Negative Control ◽  
Tubular Cell ◽  
Control Group ◽  
Tamarindus Indica ◽  
Cell Necrosis ◽  
Cell Degeneration

Aluminum is the most widely used metal in everyday and the exposure of aluminum can be accumulate in kidney tubular cells, which cause tubular cell degeneration through oxidative stress because of nephrotoxicity. Tamarindus indica seeds contain high polyphenolic compounds and high antioxidant potential. This study aimed to investigate whether the methanolic extract of Tamarindus indica seeds repair histopathological damage of aluminum-induced kidney rat assessed by the parameters of tubular cell degeneration and necrosis. This experimental study was conducted at the Faculty of Medicine, University of Jember for 10 weeks using 30 male Wistar rats. Rats were divided into 5 groups; 1) K: control group was given a mixture of aquabides-saline (NaCl 0.9%), 2) K (-): negative control group was given aluminum solution (AlCl3), 3) P1: first treatment group was given aluminum solution (AlCl3) and extract (25 mg/kg b.w., po),  4) P2: second treatment group was given aluminum solution (AlCl3) and extract (50 mg/kg b.w., po), 5) P3: third treatment group was given aluminum solution (AlCl3) and extract (100 mg/kg b.w., po). Aluminum solution is given in a dose of 300 mg/kg b.w. po. After being terminated, the rat’s kidney histology were examined to a scoring damage process. Based on cell degeneration parameter, all groups get 5 in score of damage. Meanwhile based on cell necrosis parameter, all group get different score of damage. Based on the cell necrosis parameter, the methanolic extract of Tamarindus indica seeds repair histopathological damage of rat kidney induced by aluminum chloride

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