scholarly journals Effect of Elephantopus Scaber Linn. leaf extract on mouse immune system

2021 ◽  
Vol 18 (10) ◽  
pp. 2045-2050
Author(s):  
Yufri Aldi ◽  
Megaraswita ◽  
Dwisari Dillasamola
Keyword(s):  
Immune System ◽  
Leaf Extract ◽  
Leukocyte Count ◽  
Methyl Cellulose ◽  
Ethanol Extract ◽  
Total Leukocyte Count ◽  
Macrophage Activity ◽  
Group I ◽  
Elephantopus Scaber ◽  
Leukocyte Counts

Purpose: To investigate the effect of Elephantopus Scaber Linn. ethanol extract on mouse immune system based on macrophage phagocytic activity and capacity, total leukocyte count, and distribution of each type of leukocyte. Methods: Twenty male mice were randomly assigned to four groups. Group I (control) received sodium carboxy methyl cellulose (Na-CMC), 0.5 %). Groups II, III, and IV were dosed orally with 10, 30, and 100 mg/kg ethanol E. scaber leaf extract, respectively, for 7 consecutive days. On the 8th day, a suspension of Staphylococcus aureus was injected intraperitoneally, and macrophage activity and capacity as well as leukocyte count were then measured using a counting chamber device (hemocytometer) and a 400 x microscope. Results: The effect of the leaf extract at doses of 10, 30, and 100 mg/kg was increase in macrophage activity by 46.25, 54, and 65 %, and macrophage capacity by 95.8, 105.4 and 125.8 cell/100 macrophage, respectively; total leukocyte count was 10790, 12360, and 15230 cells/µl, respectively, and lymphocyte was 34.4, 34.6, and 36 %. Thus, the leaf extract significantly increased macrophage activity and capacity, as well as segmented neutrophils, and mice total leukocyte counts (p < 0.05). Conclusion: Ethanol extract of E scaber leaf extract increases macrophage activity and capacity, as well as total leukocyte count in mouse; therefore, this extract may provide a highly effective approach for boosting immunity.

scholarly journals Immunostimulatory properties of bigroot geranium (Geranium macrorrhizum L.) extract

Medicina ◽  
2007 ◽  
Vol 43 (1) ◽  
pp. 60 ◽  
Author(s):  
Vilma Jurkštienė ◽  
Anatolijus Kondrotas ◽  
Egidijus Kėvelaitis
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Peripheral Blood ◽  
Leukocyte Count ◽  
Ethanol Extract ◽  
Food Supplement ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Case Group ◽  
Cell Counts

The aim of the study was to investigate the immunostimulatory properties of bigroot geranium. Material and methods. Possible nonspecific characteristics of bigroot geranium were evaluated by the total leukocyte count in the peripheral blood, and qualitative changes of blood were assessed using Shilling’s formula by evaluating changes in lymphocyte counts. In addition, we also studied changes in the counts of Tcell precursors in the thymus and B lymphocytes in the spleen. Ethanol extract of the leaves of bigroot geranium was produced at the Department of Food Technology, Kaunas University of Technology. Studies were performed on mice Bl 57 (n=21). The control group (n=7) received distilled water at a dose of 1 mL/day. The second and third groups received 1% and 10% extract of bigroot geranium, respectively, as a food supplement. Changes in cell counts were investigated after 4 weeks following the initiation of the trial. Results. After a 4-week administration of 1% extract of bigroot geranium (1 mL/day) (mice group, n=7), leukocyte count in the peripheral blood increased to 6.1×109 cells/L, and lymphocyte count – to 70%, but changes were not statistically significant. The other case group of mice (n=7) received 10% extract of bigroot geranium for 4 weeks at a dose of 1 mL/day. In this group, leukocyte count in the peripheral blood increased statistically significantly from 4.4×109 cells/L to 7.2×109 cells/L (p<0.01), and lymphocyte percentage – from 52% to 80% (p<0.001), as compared to control. Thymocyte (T lymphocytes) counts in thymus and splenocyte (B lymphocytes) counts in the spleen showed a tendency to increase after the administration of 1% and 10% extracts. After a 4-week administration of 1% extract of bigroot geranium, thymocyte and splenocyte counts increased from 0.342×106 cells to 0.372×106 cells per mg of tissue and from 0.395×106 cells to 0.405×106 cells per mg of tissue, respectively, as compared to control group (p>0.1). After the administration of 10% extract of bigroot geranium, thymocyte count increased to 0.488×106 cells per mg of tissue (p<0.01), and splenocyte count – to 0.504×106 cells per mg of tissue (p<0.01). Conclusion. The extracts of the leaves of bigroot geranium increased leukocyte count and lymphocyte percentage in the peripheral blood, and after a 4-week administration of 10% extract of bigroot geranium, a statistically significant increase in the counts of T lymphocytes (in the thymus) and B lymphocytes (in the spleen) was observed. The immunostimulatory effect depends on the dose of the extract.

scholarly journals THE EFFECTIVENESS OF ETHANOL EXTRACT OF BINAHONG LEAVES ON DIABETIC WOUND HEALING

2018 ◽  
Vol 11 (4) ◽  
Author(s):  
Devita Anggraeni ◽  
Claude Mona Airin ◽  
Slamet Raharjo
Keyword(s):  
Blood Glucose ◽  
Leukocyte Count ◽  
Ethanol Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Dorsal Skin ◽  
Group I ◽  
Diabetic Control Group ◽  
Chemical Profiles

This research aimed to study the effectiveness of ethanol extract of binahong leaves on blood glucose, insulin, blood chemical profiles (serum glutamic pyruvate transaminase=SGPT, serum glutamic oxaloacetic transaminase=SGOT, ureum, and creatinine), and skin histopathology in diabetic rat. A total of 20 male Wistar rats aged 3 months (± 250 gram) were divided into five groups, with four rats in each group. Group I (non-diabetic control) was injected with 0.1 M sodium citrate buffer, while group II (diabetic control), III, IV, and V were injected with single dose of Streptozotocin (STZ) at dose 40 mg/kg intraperitoneally (IP). One week after the injection, the dorsal skin of the rats were excised. Group I and II were given cream topically and 1% NaCMC orally, group III was given 50% ethanol extract of binahong leaves (EEB) topically and 1% NaCMC orally, group IV was given cream topically and EEB 300 mg/kg orally, and group V was given 50% EEB topically and EEB 300 mg/kg orally. These treatments were continued for 14 days. Blood samples were obtained at the end of study to examine blood glucose, insulin, and blood chemical profiles (SGOT, SGPT, ureum, and creatinine). Examination of skin histopathology and leukocyte count were also done. The result showed that blood glucose, insulin, SGOT, SGPT, and ureum level of diabetic rats given topical or oral EEB did not significantly different from diabetic control group, even though blood glucose, insulin, SGOT, SGPT, and ureum level of diabetic rats given topical and oral EEB were found lower compared to diabetic control group. Administration of EEB 300 mg/kg orally in diabetic rats could lower creatinine level significantly (P<0.05). Histopathological examination of dorsal skin of diabetic rats which were given EEB topically showed the decrease of fibroblast proliferation, leukocyte infiltration, and hemorrhage in dermis area. Leukocyte count on skin tissue was significantly lower (P<0.05) in diabetic rats given EEB. In conclusion, topical or oral administration of EEB can help healing process in diabetic wound.

Shift Work and Leukocyte Count Changes among Workers in Bangkok

2019 ◽  
Vol 63 (6) ◽  
pp. 689-700
Author(s):  
Nitt Hanprathet ◽  
Somrat Lertmaharit ◽  
Vitool Lohsoonthorn ◽  
Thanapoom Rattananupong ◽  
Palanee Ammaranond ◽  
...  
Keyword(s):  
Shift Work ◽  
Immune Cell ◽  
Leukocyte Count ◽  
Total Leukocyte Count ◽  
Percentage Increase ◽  
Cross Sectional ◽  
Work Exposure ◽  
Leukocyte Counts ◽  
Current Shift

Abstract Objectives Previous epidemiological evidence for the association of shift work exposure and increased leukocyte count is cross-sectional in nature, thus limiting cause-effect inference. We therefore used a longitudinal design to: (i) compare leukocyte counts at baseline between shift and day workers and (ii) examine the time trend of leukocyte counts over the follow-up period for these workers. Methods A retrospective cohort study was conducted among 6737 workers aged <60 years at two large organizations (a humanitarian organization and a university) in Bangkok, Thailand who had participated in at least two annual health check-ups during the period 2005–2016. Shift work exposure history was assessed by a self-administered questionnaire and categorized into day, former, and current shift workers. Data on leukocyte count were collected annually as part of worksite health screening during the observation period. Association of shift work exposure and increased leukocyte count was then examined cross-sectionally and longitudinally by using multiple linear regression and multilevel analysis of repeated measures data, respectively. In addition, trends for leukocyte count over the follow-up period and work years were examined using LOWESS smooth curves. Results Compared to day work, the current shift work was associated with increased leukocyte counts. The magnitude of percentage increase was the highest for basophil counts, followed by eosinophil and lymphocyte counts. Both cross-sectional and longitudinal evidence revealed this association, although it was less pronounced longitudinally. For total leukocyte count, the magnitude of difference was constant across the 11-year follow-up period. However, for lymphocyte and basophil counts, these discrepancies tapered over the work years until they no longer differed (for lymphocyte count) or even differed in the opposite direction (for basophil count) in later work years. Conclusion This study confirmed previous cross-sectional evidence that shift work exposure-increased leukocyte counts and that this was reversible. Whether this increase in immune cell count also results in an increased immune cell activity and serves as the intermediary in the association between shift work exposure and subsequent chronic disease development needs further investigation.

Abstract P45: White Blood Cell Counts in Aneurysmal Subarachnoid Hemorrhage Patients as a Predictor of Cardiac Injury

Stroke ◽  
2021 ◽  
Vol 52 (Suppl_1) ◽  
Author(s):  
Tiffany Cheng ◽  
Joseph R Geraghty ◽  
Neil S Saini ◽  
Yonatan Hirsch ◽  
Tyler J Lung ◽  
...  
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Cardiac Dysfunction ◽  
Leukocyte Count ◽  
Cardiac Injury ◽  
Total Leukocyte Count ◽  
Monocyte Count ◽  
Cell Counts ◽  
Leukocyte Counts ◽  
Blood Leukocyte ◽  
The Relationship

Introduction: Up to 50% of subarachnoid hemorrhage (SAH) patients develop cardiac injury. The relationship between early systemic inflammation and cardiac injury after SAH is unknown. Here we examined changes in blood leukocyte counts and their relationship to cardiac dysfunction. Methods: We reviewed the medical records of 288 SAH patients admitted to our Comprehensive Stroke Center. Patients were dichotomized based on elevated (≥0.04ng/mL) or normal (<0.04ng/mL) troponin I (TnI). Demographics and labs from admission were then compared among the two groups by Chi-Square or Mann-Whitney test. Ejection fraction (EF) was stratified into low (<50%), normal (50-70%), or high (>70%) from echocardiogram data. We performed univariate and multivariate logistic regression to establish the relationship between blood leukocyte counts and cardiac injury. Results: Of 288 SAH patients, 241 had TnI levels performed at the time of admission and 119 (49.4%) of these had elevated TnI on admission. Patients with elevated TnI were significantly older, had higher grade SAH, abnormal EF, and were more likely to have hypertension and dyslipidemia. 10 (4.1%) had low EF while 58 (24.1%) had high EF on admission echocardiogram. In univariate analysis, total leukocyte count (p<0.0001), absolute neutrophil count (p=0.037), absolute monocyte count (p=0.014), and neutrophil-lymphocyte ratio (p=0.010) were associated with elevated TnI. Multivariate analysis adjusting for covariates showed that only total leukocyte count remained a significant predictor of elevated TnI (OR = 1.104, 95% CI= 1.020 - 1.195; p=0.014). Receiver operating characteristic (ROC) analysis demonstrated that adjusted total leukocyte count distinguishes between SAH patients with normal and elevated TnI (area under the curve = 0.787, p=0.001), with the optimal cutoff being 0.521 (sensitivity of 67.0% and specificity of 80.6%). Conclusions: Blood total leukocyte count is an independent predictor of cardiac injury in SAH patients. This highlights the role of inflammation in mediating cardiac dysfunction after brain hemorrhage, and raises questions regarding the potential of anti-inflammatory therapy for cardioprotection in SAH.

scholarly journals Evaluation of predictive value of total leukocyte count in acute serious bacterial infections in children 1 month to 5 years

2017 ◽  
Vol 4 (2) ◽  
pp. 415
Author(s):  
Dharmalingam A. ◽  
Vignesh C. ◽  
Raghupathy N. S. ◽  
Sowmiya M.
Keyword(s):  
Tract Infection ◽  
Antibiotic Therapy ◽  
Bacterial Infection ◽  
Leukocyte Count ◽  
Empiric Antibiotic Therapy ◽  
Total Leukocyte Count ◽  
Febrile Child ◽  
Group I ◽  
The Mean ◽  
Group 2

Background: Fever in a young child is a frequently encountered clinical problem with various causes. Most of them run a benign self-limiting course that requires only symptomatic treatment. However, in a few children the underlying etiology can be a life threatening serious bacterial infection (SBI). Early identification of SBI is warranted because of the need to start antibiotic therapy, often empirical, as soon as possible to prevent morbid sequela. Total leukocyte count (TLC) is with neutrophil predominance is considered to be a surrogate marker of a bacterial infection and facilitating decision - making regarding further evaluation and empiric antibiotic therapy. The purpose of this study was to determine the utility of leukocyte in predicting SBI in a young febrile child and to correlate the leukocyte count with non-serious infection.Methods: One fifty children, age of 1 month to 60 months with fever > 38°c are included and were analyzed for demographic details, presenting symptoms, physical examination findings, clinical diagnosis, total leukocyte count, peripheral smear study, blood culture, stool culture, CSF analysis, x-ray chest, abdominal ultrasound, CT-brain. Results: Out of 150 children included in the study, the mean age was 26.7±14.54 months and the average duration of fever was 4.3±1.02 days. The mean leukocyte count was 15016.43±5801.98 cells/cu.mm. 74 children had proven serious bacterial infection (49.3%) and were categorized in Group I and remaining non-serious infection were 50.66% are belonged to Group 2. Urinary tract infection and pneumonia were the most common SBI encountered (31/20/68.91%). The highest TLC counts were seen in children with UTI. Analysis of variance (ANNOVA) did not reveal significant differences in TLC between children with different diagnosis. In Group 2, majority of the etiology was lower respiratory tract infection/inflammation and short febrile illness.Conclusions: SBI comprise only half of the febrile children with leukocytosis. The predictive accuracy of leukocytosis in diagnosing SBI is poor. Vomiting and seizures probably incite stress mediated leukocytosis and are commonly encountered in febrile child with a high TLC without SBI. Further models with addition of clinical data and other surrogates of infection need to be developed to improve prediction of SBI in a febrile child.  

scholarly journals Determination of Impact of Acute and Chronic Administration of Aqueous Root Extract of E. Tremula (Burburwa) on some Haematological parameters in Wistar Albino Rats

2021 ◽  
Vol 6 (3) ◽  
pp. 115-126
Author(s):  
H.M. Ahmed ◽  
A. Yakubu ◽  
S.R. Ismail ◽  
A.B. Ibrahim ◽  
A. Salahudden ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Toxicity Study ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Albino Rats ◽  
Root Extract ◽  
Group I ◽  
Leukocyte Counts ◽  
The Impact ◽  
Aqueous Root Extract

Eragrostis tremula (Burburwa) is an herbal plant native to tropical Africa, India and Myanmar. It is found in sandy soils and abandoned cultivations. It is used locally for sweeping, used as fodder for cattle and extensively being used in traditional medicine practice without assessing its safety. This study investigated the impact on blood cells (WBCs) on chronic/acute use of aqueous root extract of Eragrostis tremula among males/females wistar rats. The preliminary phytochemical screening revealed the presence of alkaloids, carbohydrate, phenols, saponins, tannins, anthraquinones, cardiac glycosides, flavonoids and diterpene. Lorke's method was used for oral acute toxicity study (LD50 determination). A total of 20 wistar rats were used, divided into four groups of five rats each. Group I was control and administered with distilled water, Group II, Group III and Group IV were respectively administered with 200 mg, 400 mg and 800 mg of the extract per kilogram body weight, for 21 days. The result of acute oral toxicity study was estimated to be greater than 5000 mg/kg. Total and differential (neutrophil, lymphocyte, and mixed cells) leukocyte counts were analysed. Eragrostis tremula aqueous root extract significantly (p<0.05) decreased lymphocyte and total leukocyte counts, but no significant changes (p>0.05) were observed in neutrophil and mixed cells count when the treated groups were compared with the control group. In conclusion, the findings of the present study have shown that, aqueous root extract of Eragrostis tremula has impact on lymphocyte and total leukocyte count in the wistar rats which is dependent on dose of the extract. The extract at high doses increases the lymphocyte and total leukocyte counts of the treated groups.

The effect of fermented goat milk (kefir) fortified with vitamin D3 on total leukocyte levels in diabetic Rattus norvegicus rats

2019 ◽  
Vol 50 (2) ◽  
pp. 324-332 ◽  
Author(s):  
Reny Marlina ◽  
Binar Panunggal ◽  
Gemala Anjani
Keyword(s):  
Blood Glucose ◽  
Vitamin D3 ◽  
Rattus Norvegicus ◽  
Leukocyte Count ◽  
Goat Milk ◽  
Total Leukocyte Count ◽  
Content Type ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Leukocyte Counts

Purpose Hyperglycemia in type 2 diabetes mellitus increases inflammation through pancreatic beta-cell destruction. Goat milk (kefir) and vitamin D3 have antioxidant and anti-inflammatory properties that can reduce pancreatic beta cells. Increase in total leukocyte count is one of the inflammatory and complication markers of diabetes. This study aims to analyze the effect of fermented goat milk (kefir) fortified with vitamin D3 on total leukocyte counts in diabetic brown rat (Rattus norvegicus). Design/methodology/approach An experimental design with pre- and post-test methodology of control and experimental groups was used. A total of 24 male Wistar rats were divided into four groups: negative control (K−), positive control (K+), kefir treatment (P1) and kefir fortified with vitamin D3 treatment (P2). The control group consisted of healthy rats; the other groups consisted of rats which were given streptozotocin–nicotinamide injections. Before and after intervention, fasting blood glucose levels and total leukocytes counts were measured. Blood glucose levels were analyzed using the GOD–PAP method and leukocyte counts were analyzed using an automatic hematological analyzer. Findings There was a significant decrease in total leukocyte counts in the P2 group (from 26.5 ± 7.7 to 20.4 ± 10.4 [p < 0.05]). However, the decrease in leukocyte count in theP1 group was not significant (from 22.1 ± 9.3 to 16.5 ± 6.2). The decrease in blood glucose levels in theP2 group was −81.4 ± 203.0 but not significant. Originality/value Kefir with vitamin D3 fortification can significantly reduce total leukocyte counts in diabetes.

scholarly journals Role of total leukocyte count in diagnosis of pediatric acute appendicitis: effect of antibiotic administration and duration of onset of symptoms

2017 ◽  
Vol 5 (1) ◽  
pp. 296
Author(s):  
Arun M. Ainippully ◽  
Sarath Kumar Narayanan ◽  
Vysakh C. N. ◽  
Arun Preeth V. ◽  
Prathap Somnath
Keyword(s):  
Leukocyte Count ◽  
Total Leukocyte Count ◽  
Symptom Duration ◽  
Antibiotic Administration ◽  
Group Iii ◽  
Group I ◽  
Routine Work ◽  
Number Of Patients ◽  
Group A ◽  
Group B

Background: Appendicitis is one of the most common pediatric surgical emergencies. The aim of the study is to determine the significance of total leukocyte count (TLC) in diagnosing appendicitis in the present era of rampant and injudicious antibiotic usage, and its relation to the duration of onset of symptoms.Methods: Study population consists of 148 pediatric patients who had confirmed appendicitis and underwent appendicectomy between January 2015 and December 2015. TLC was performed in all these patients as part of the routine work up. The population was categorized as: group I <24hrs, group II 24 to 4hrs and group III >48 hrs, based on symptom duration and group A and B based on antibiotics received or not respectively.Results: 64.9% (n = 96) were males and 35.1% (n = 52) were females. The ages ranged from 2.1 to 12.9 years (mean/SD being 8.3±1.2). The number of patients with elevated TLC was significantly lesser in Group B (62.2%, n = 92) when compared to the group A (37.8%, n = 56) which did not receive antibiotics (P <0.017). Significantly higher number of patients showed rise in TLC if it was done within 24 hours of onset of symptoms (66.6%, n =40).Conclusions: Our study shows that TLC proved more useful if it is performed within 24 hours of the onset of symptoms and is relevant only if antibiotics have not been administered.

Evaluation of Serum C-Reactive Protein, Plasma Fibrinogen, and Blood Leukocytes in Patients with Chronic Periodontitis

2021 ◽  
Vol 5 (1) ◽  
pp. 11-18
Author(s):  
Shristi Kafle ◽  
Erika Shrestha ◽  
Sanjay Yadav
Keyword(s):  
Cardiovascular Diseases ◽  
Chronic Periodontitis ◽  
Healthy Subjects ◽  
Leukocyte Count ◽  
Plasma Fibrinogen ◽  
Total Leukocyte Count ◽  
P Value ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Leukocyte Counts

Introduction: Periodontal health is crucial and indispensable element of general health. Epidemiologically, it has been linked with cardiovascular diseases with elevated acute phase reactant C-reactive protein, fibrinogen and rheological variables such as total leukocyte count and differential leukocyte counts, which are potential predictors of cardiovascular diseases. Objective: To evaluate and compare cardiovascular disease-related biochemical markers in periodontally healthy subjects, and patients with moderate and severe chronic periodontitis. Methods: An analytical cross-sectional study was conducted in patients attending Department of Periodontology and Oral Implantology, Chitwan Medical College and Teaching hospital, Bharatpur, Chitwan, Nepal from September 2019 to January 2021. A total of 81 individuals of both gender of which 27 periodontally healthy individuals, 27 diagnosed with moderate and 27 diagnosed with severe periodontitis based on gingival index, probing pocket depth and clinical attachment level were enrolled in a study. After which, peripheral blood samples were drawn and serum C-reactive protein, plasma fibrinogen, total leukocyte count and differential leukocyte counts were quantified using the turbidimetric immunoassay. Convenience sampling technique was done. Data was analysed in SPSS v.26.0. Results: The mean serum levels of C-reactive protein, plasma fibrinogen, total leukocyte and differential leukocytes were found to be statistically (P value <0.001) higher in severe and moderate periodontitis subjects compared to periodontally healthy subjects. Conclusion: The increased levels of serum C-reactive protein, plasma fibrinogen, total leukocyte count, and differential leukocyte counts in chronic periodontitis contribute to the inflammatory burden of the individual potentially striking toward an increasing risk for cardiovascular events.  

scholarly journals The resistance of sangkuriang catfish (Clarias gariepinus Burchell 1822) against Aeromonas hydrophila bacteria given moringa leaf extracts (Moringa oleifera L.) through the feed

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
. Rosidah ◽  
Ibnu Dwi Buwono ◽  
Walim Lili ◽  
Ibnu Bangkit Suryadi ◽  
Ade Reza Triandika
Keyword(s):  
Survival Rate ◽  
Clinical Sign ◽  
Aeromonas Hydrophila ◽  
Leaf Extract ◽  
Leukocyte Count ◽  
Clarias Gariepinus ◽  
Total Leukocyte Count ◽  
Leaf Extracts ◽  
Significance Level ◽  
Moringa Leaf Extract

This study aims to reveal effective concentration of moringa leaf extract which is inducing body resistance of sangkuriang catfish against Aeromonas hydrophila. This study used as experimental method of Completely Randomized Design (CRD) with six treatments and three replications. The treatment used in this research are the addition of Moringa leaf extract into feed with concentration of 0 ppm (A), 100 ppm (B), 125 ppm (C), 150 ppm (D), 175 ppm (E) and 200 ppm (F). Observed Parameters are total leukocyte count, increase in leukocyte, clinical sign and survival rate.  Increase in leukocyte and survival rate data were analyzed using F test and Duncan test with significance level 0,05, while clinical sign data was analyzed descriptively. The results showed that Moringa leaf extract was effective to induce the immune of sangkuriang catfish. The 150 ppm concentration resulted the highest total leukocyte count of 23.46 ± 6.46% and didn’t have clinical symptons after challenge with Aeromonas hydrophila and highest survival rate 80.0 ± 5.00%. Based on the regression analysis, 153.95 ppm of Moringa leaf extract is the optimum concentration with 95.62% on the survival of sangkuriang catfish after challenge with Aeromonas hydrophila.AbstrakPenelitian ini bertujuan untuk menentukan konsentrasi yang efektif dari ekstrak daun kelor untuk menginduksi ketahanan tubuh ikan lele sangkuriang terhadap serangan bakteri Aeromonas hydrophila. Penelitian ini menggunakan metode eksperimental Rancangan Acak Lengkap dengan enam perlakuan dan tiga ulangan. Perlakuan penelitian yang digunakan adalah penambahan ekstrak daun kelor ke dalam pakan dengan konsentrasi 0 ppm (A), 100 ppm (B), 125 ppm (C), 150 ppm (D), 175 ppm (E) dan 200 ppm (F). Parameter yang diamati meliputi jumlah total sel darah, peningkatan jumlah sel darah putih, gejala klinis, dan sintasan. Data peningkatan jumlah sel darah putih dan sintasan dianalisis menggunakan uji F dan uji jarak berganda pada taraf kepercayaan 5%, sedangkan data gejala klinis dianalisis secara deskriptif. Hasil penelitian menunjukkan ekstrak daun kelor efektif untuk menginduksi ketahanan tubuh benih lele sangkuriang. Konsentrasi 150 ppm menghasilkan rata-rata jumlah sel darah putih tertinggi 23,46±6,46% dan setelah diuji tantang tidak menunjukkan gejala klinis, serta tingkat sintasan yang tertinggi sebesar 80,0±5,00%. Berdasarkan analisis regresi konsentrasi ekstrak daun kelor memberikan pengaruh sebesar 95,62% dengan konsentrasi optimum sebesar 153,95 ppm terhadap sintasan ikan lele sangkuriang setelah di uji tantang dengan Aeromonas hydrophila.

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